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ABSTRACT We conducted the genome sequencing and analysis of the first confirmed COVID-19 infections in Brazil. Rapid sequencing coupled with phylogenetic analyses in the context of travel history corroborate multiple independent importations from Italy and local spread during the initial stage of COVID-19 transmission in Brazil.

https://doi.org/10.1590/s1678-9946202062030 3197 downloads

Molecular epidemiology of influenza A(H1N1)PDM09 hemagglutinin gene circulating in São Paulo State , Brazil: 2016 anticipated influenza season
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Santos, Katia Corrêa de Oliveira

; Silva, Daniela Bernardes Borges da

; Sasaki, Norio Augusto

; Benega, Margarete Aparecida

; Garten, Rebecca

; Paiva, Terezinha Maria de

Revista do Instituto de Medicina Tropical de São Paulo

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2017, Volume 59 elocation e9

Abstract: En | Text: En | PDF: En

ABSTRACT Compared to previous years, seasonal influenza activity commenced early in São Paulo State, Brazil, Southern hemisphere during the 2016 year. In order to investigate the genetic pattern of influenza A(H1N1)pdm09 in the State of Sao Paulo a total of 479 respiratory samples, collected in January by Sentinel Surveillance Units, were screened by real-time RT-PCR. A total of 6 Influenza viruses A(H1N1)pdm09 presenting ct values ≤ 30 were sequenced following phylogenetic analysis. The present study identified the circulation of the new 6B.1 subgroup (A/Sao Paulo/10-118/2016 and A/Sao Paulo/3032/2016). In addition, influenza A(H1N1)pdm09 group 6B has also been identified during January in the State of Sao Paulo. Despite amino acid changes and changes in potential glycosylation motifs, 6B.1 viruses were well inhibited by the reference ferret antiserum against A/California/07/2009 virus, the A(H1N1)pdm09 component of the vaccine for the 2016 influenza season.

https://doi.org/10.1590/s1678-9946201759009 1484 downloads

Use of molecular test for adenovirus detection between different pediatric patients
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Paiva, Terezinha Maria de

Revista Paulista de Pediatria

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jun 2015, Volume 33 N. 2 Pages 134 - 135

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1016/j.rpped.2015.03.005 2214 downloads

INFLUENZA VIRUS SURVEILLANCE BY THE INSTITUTO ADOLFO LUTZ, INFLUENZA SEASON 2014: ANTIVIRAL RESISTANCE
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Santos, Katia Corrêa de Oliveira

; Silva, Daniela Bernardes Borges da

; Benega, Margarete Aparecida

; Paulino, Renato de Sousa

; Silva Jr, Elian Reis E

; Pereira, Dejanira dos Santos

; Mussi, Aparecida Duarte Hg

; Silva, Valéria Cristina da

; Gubareva, Larissa V.

; Paiva, Terezinha Maria de

Revista do Instituto de Medicina Tropical de São Paulo

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feb 2015, Volume 57 N. 1 Pages 92 - 92

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/S0036-46652015000100016 1488 downloads

MOLECULAR CHARACTERIZATION OF INFLUENZA B VIRUS OUTBREAK ON A CRUISE SHIP IN BRAZIL 2012
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Borborema, Samanta Etel Treiger

; Silva, Daniela Bernardes Borges da

; Silva, Kátia Corrêa Oliveira

; Pinho, Margarete Aparecida Benega

; Curti, Suely Pires

; Paiva, Terezinha Maria de

; Santos, Cecília Luiza Simões

Revista do Instituto de Medicina Tropical de São Paulo

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jun 2014, Volume 56 N. 3 Pages 185 - 189

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Em fevereiro de 2012, durante a temporada de verão no Brasil, um surto de doença respiratória ocorreu no navio de cruzeiro MSC Armonia. Mulher de 31 anos, membro da tripulação, foi internada com insuficiência respiratória e morreu. Com o objetivo de estudar a etiologia da doença foram investigadas necrópsia de tecido do caso fatal e secreções respiratórias de 13 passageiros e membros da tripulação com sintomas respiratórios. O teste de influenza por RT-PCR em tempo real foi realizado e o gene completo da hemaglutinina (HA) das amostras positivas foi sequenciado. O vírus influenza B foi detectado em sete indivíduos, sugerindo-o como a causa do surto de doença respiratória a bordo do navio. A análise da sequência do gene da HA indicou que os vírus estão fortemente relacionados com o vírus B/Brisbane/60/2008, linhagem Victoria, componente da vacina de influenza para 2011-2012 no hemisfério sul. Uma vez que a composição da vacina foi alterada para uso na temporada de 2012-2013, é essencial a vigilância ativa dos vírus circulantes em todo o mundo. A análise molecular é uma ferramenta importante para caracterização do patógeno responsável pelo surto. Além disso, a vigilância de doenças baseada em dados laboratoriais contribui para as medidas de controle da influenza, uma doença imunoprevinível.

In February 2012, an outbreak of respiratory illness occurred on the cruise ship MSC Armonia in Brazil. A 31-year-old female crew member was hospitalized with respiratory failure and subsequently died. To study the etiology of the respiratory illness, tissue taken at necropsy from the deceased woman and respiratory specimens from thirteen passengers and crew members with respiratory symptoms were analyzed. Influenza real-time RT-PCR assays were performed, and the full-length hemagglutinin (HA) gene of influenza-positive samples was sequenced. Influenza B virus was detected in samples from seven of the individuals, suggesting that it was the cause of this respiratory illness outbreak. The sequence analysis of the HA gene indicated that the virus was closely related to the B/Brisbane/60/2008-like virus, Victoria lineage, a virus contained in the 2011-12 influenza vaccine for the Southern Hemisphere. Since the recommended composition of the influenza vaccine for use during the 2013 season changed, an intensive surveillance of viruses circulating worldwide is crucial. Molecular analysis is an important tool to characterize the pathogen responsible for an outbreak such as this. In addition, laboratory disease surveillance contributes to the control measures for vaccine-preventable influenza.

https://doi.org/10.1590/s0036-46652014000300001 2446 downloads

Myenteric plexus is differentially affected by infection with distinct Trypanosoma cruzi strains in Beagle dogs
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Nogueira-Paiva, Nívia Carolina

; Fonseca, Kátia da Silva

; Vieira, Paula Melo de Abreu

; Diniz, Lívia Figueiredo

; Caldas, Ivo Santana

; Moura, Sandra Aparecida Lima de

; Veloso, Vanja Maria

; Guedes, Paulo Marcos da Matta

; Tafuri, Washington Luiz

; Bahia, Maria Terezinha

; Carneiro, Cláudia Martins

Memórias do Instituto Oswaldo Cruz

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feb 2014, Volume 109 N. 1 Pages 51 - 60

Abstract: En | Text: En | PDF: En

Chagasic megaoesophagus and megacolon are characterised by motor abnormalities related to enteric nervous system lesions and their development seems to be related to geographic distribution of distinct Trypanosoma cruzi subpopulations. Beagle dogs were infected with Y or Berenice-78 (Be-78) T. cruzi strains and necropsied during the acute or chronic phase of experimental disease for post mortem histopathological evaluation of the oesophagus and colon. Both strains infected the oesophagus and colon and caused an inflammatory response during the acute phase. In the chronic phase, inflammatory process was observed exclusively in the Be-78 infected animals, possibly due to a parasitism persistent only in this group. Myenteric denervation occurred during the acute phase of infection for both strains, but persisted chronically only in Be-78 infected animals. Glial cell involvement occurred earlier in animals infected with the Y strain, while animals infected with the Be-78 strain showed reduced glial fibrillary acidic protein immunoreactive area of enteric glial cells in the chronic phase. These results suggest that although both strains cause lesions in the digestive tract, the Y strain is associated with early control of the lesion, while the Be-78 strain results in progressive gut lesions in this model.

https://doi.org/10.1590/0074-0276130216 2676 downloads

DIFFERENTIAL DIAGNOSIS OF RESPIRATORY VIRUSES BY USING REAL TIME RT-PCR METHODOLOGY
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Paulino, Renato de Souza

; Benega, Margarete Aparecida

; Santos, Katia Corrêa de Oliveira

; Silva, Daniela Bernardes Borges da

; Pereira, Juliana Cristina

; Sasaki, Norio Augusto

; Silva, Patricia Evelin

; Curti, Suely Pires

; Oliveira, Maria Isabel

; R.M.P. Carvalhanas, Telma

; Peret, Teresa

; Erdman, Dean

; Paiva, Terezinha Maria de

Revista do Instituto de Medicina Tropical de São Paulo

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dec 2013, Volume 55 N. 6 Pages 432 - 432

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/S0036-46652013000600012 2390 downloads

Analysis of the relationship between EEG signal and aging through Linear Discriminant Analysis (LDA)
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Paiva, Lilian Ribeiro Mendes de

; Pereira, Adriano Alves

; Almeida, Maria Fernanda Soares de

; Cavalheiro, Guilherme Lopes

; Milagre, Selma Terezinha

; Andrade, Adriano de Oliveira

Revista Brasileira de Engenharia Biomédica

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jun 2012, Volume 28 N. 2 Pages 155 - 168

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo deste trabalho é estabelecer as correlações entre parâmetros estatísticos e EEG em função da idade, em indivíduos não portadores de distúrbios neurológicos. Os sinais EEG foram registrados durante a tarefa de seguir uma espiral de Arquimedes. 59 indivíduos saudáveis participaram do estudo e foram divididos em 7 grupos, com idades entre 20 a 86 anos, de ambos os sexos, para identificar diferenças e permitir a discriminação entre as características de cada grupo. Inicialmente, foram feitas comparações entre as diversas variáveis (F20, F50, F80, F95, Frequência Média, RMS, Cruzamentos por zero, Quadrado do Espectro de Potência, Curtose, Assimetria, Variância, Desvio Padrão e Entropia Aproximada) procurando a separação entre os grupos jovem e idoso. Buscou-se ainda correlacionar os parâmetros estatísticos e toda a faixa etária. Para tal, a técnica de Análise Discriminante Linear (ADL) foi utilizada. Os dados foram processados com o software MATLAB®. Por meio da ADL foram observadas diferenças significativas ao longo da idade. Observou-se que a ferramenta executou de forma satisfatória a separação de características discriminantes, classificando cada grupo de indivíduos em função da idade.

This paper aims to establish the correlation between statistical parameters and Electroencephalographic (EEG) signals as a function of age, in subjects without neurological disorders. EEG signals were recorded during the task of following an Archimedes spiral. There were 59 healthy subjects who voluntarily participated in this study which were divided into 7 groups, aging between 20 to 86 years from both gender, in order to identify differences and allow discrimination between the features of each group. Initially, comparisons were made among several features (F20, F50, F80, F95, Mean Frequency, Root Mean Square value, Zero Crossings, Square of the Power Spectrum, Kurtosis, Skewness, Variance, Standard Deviation and Approximate Entropy) seeking separation between young and elderly groups. Furthermore, it was sought to correlate the statistical parameters and the entire age range. For this purpose it was used Linear Discriminant Analysis (LDA). The data were processed with MATLAB® software. Through the LDA, significant differences were observed over the distinct age ranges. The tool has satisfactorily performed the separation of discriminant features by classifying groups of subjects in function of their age range.

https://doi.org/10.4322/rbeb.2012.023 5267 downloads

Sequence analysis of the 2009 pandemic influenza A H1N1 virus haemagglutinin gene from 2009-2010 Brazilian clinical samples
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Ferreira, João Leandro de Paula

; Borborema, Samanta Etel Treiger

; Brígido, Luis Fernando de Macedo

; Oliveira, Maria Isabel de

; Paiva, Terezinha Maria de

; Santos, Cecília Luiza Simões dos

Memórias do Instituto Oswaldo Cruz

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aug 2011, Volume 106 N. 5 Pages 613 - 616

Abstract: En | Text: En | PDF: En

In this paper, we analysed the haemagglutinin (HA) gene identified by polymerase chain reaction from 90 influenza A H1N1 virus strains that circulated in Brazil from April 2009-June 2010. A World Health Organization sequencing protocol allowed us to identify amino acid mutations in the HA protein at positions S220T (71%), D239G/N/S (20%), Y247H (4.5%), E252K (3.3%), M274V (2.2%), Q310H (26.7%) and E391K (12%). A fatal outcome was associated with the D239G mutation (p < 0.0001). Brazilian HA genetic diversity, in comparison to a reference strain from California, highlights the role of influenza virus surveillance for study of viral evolution, in addition to monitoring the spread of the virus worldwide.

2395 downloads

10.

Diagnóstico de conjuntivite adenoviral pelo RPS Adenodetector®
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Barbosa Junior, José Bonifácio

; Regatieri, Caio Vinicius Saito

; Paiva, Terezinha Maria de

; Benega, Margareth Aparecida

; Ishida, Maria Akiko

; Corrêa, Kátia Oliveira

; Freitas, Denise de

; Belfort Junior, Rubens

Arquivos Brasileiros de Oftalmologia

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jun 2007, Volume 70 N. 3 Pages 441 - 444

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Avaliar a utilização do RPS Adenodetector®, como método diagnóstico de pacientes com quadro clínico de conjuntivite adenoviral. MÉTODOS: Análise de série de casos consecutivos de pacientes com diagnóstico clínico de ceratoconjuntivite adenoviral submetidos comparativamente ao teste RPS Adenodetector® e a raspado conjuntival para cultura de vírus. RESULTADOS: Dos 11 pacientes avaliados, 10 pacientes apresentavam acometimento unilateral. Em relação ao tempo de início dos sintomas no momento da colheita, 5 (45,5%) pacientes apresentavam dois dias de história, 5 (45,5%) apresentavam três dias e 1 (9,1%) apresentava 7 dias. A cultura para adenovírus foi positiva em 8 pacientes (73%) e o RPS Adenodetector® foi positivo em 9 pacientes (82%). Oito pacientes apresentaram o teste rápido e cultura positiva. Um paciente apresentou teste RPS Adenodetector® positivo com cultura negativa. Os dois pacientes com teste RPS Adenodetector® negativo apresentaram cultura negativa. O RPS Adenodetector® mostrou sensibilidade de 100% e especificidade de 67% adotando-se a cultura de vírus como exame padrão-ouro para o diagnóstico de conjuntivite adenoviral. CONCLUSÃO: O RPS Adenodetector® foi útil para o diagnóstico de conjuntivite adenoviral e pode auxiliar na orientação do paciente quanto ao contágio e disseminação da doença.

PURPOSE: To evaluate the RPS Adenodetector®, a rapid immunochromatographic test, in the diagnosis of patients with clinical overt adenoviral conjunctivitis. METHODS: Consecutive case series. Patients underwent conjunctiva scraping for RPS Adenodetector® test and culture to identify adenovirus. RESULTS: A total of 11 patients were studied, and 10 had unilateral disease. Five (45.5%) had symptoms for 2 days, 5 for three days, and 1 for 7 days. Adenovirus culture was positive in 8 patients (73%) and RPS Adenodetector® was positive in 9 (82%) patients. Eight patients had adenovirus identification by both methods. In one patient the RPS Adenodetector® was positive in contrast to a negative culture. The two patients revealing negative RPS Adenodetector® results also had negative cultures. The sensitivity was 100% and the specificity was 67%. CONCLUSION: The RPS Adenodetector® is a useful tool in the rapid diagnosis of adenovirus conjunctivitis and may contribute to the spread control of this highly contagious disease.

16651 downloads

11.

Influenza
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Forleo-Neto, Eduardo

; Halker, Elisa

; Santos, Verônica Jorge

; Paiva, Terezinha Maria

; Toniolo-Neto, João

Revista da Sociedade Brasileira de Medicina Tropical

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apr 2003, Volume 36 N. 2 Pages 267 - 274

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A influenza (gripe) é doença infecciosa aguda de origem viral que acomete o trato respiratório e a cada inverno atinge mais de 100 milhões de pessoas na Europa, Japão e Estados Unidos, causando anualmente a morte de cerca de 20 a 40 mil pessoas somente neste último país. O agente etiológico é o Myxovirus influenzae, ou vírus da gripe. Este subdivide-se nos tipos A, B e C, sendo que apenas os do tipo A e B apresentam relevância clínica em humanos. O vírus influenza apresenta altas taxas de mutação, o que resulta freqüentemente na inserção de novas variantes virais na comunidade, para as quais a população não apresenta imunidade. São poucas as opções disponíveis para o controle da influenza. Dentre essas, a vacinação constitui a forma mais eficaz para o controle da doença e de suas complicações. Em função das mutações que ocorrem naturalmente no vírus influenza, recomenda-se que a vacinação seja realizada anualmente. No Brasil, segundo dados obtidos pelo Projeto VigiGripe - ligado à Universidade Federal de São Paulo -, verifica-se que a influenza apresenta pico de atividade entre os meses de maio e setembro. Assim, a época mais indicada para a vacinação corresponde aos meses de março e abril. Para o tratamento específico da influenza estão disponíveis quatro medicamentos antivirais: os fármacos clássicos amantadina e rimantidina e os antivirais de segunda geração oseltamivir e zanamivir. Os últimos, acrescentam alternativas para o tratamento da influenza e ampliam as opções disponíveis para o seu controle.

Influenza (flu) is an acute contagious viral infection characterized by inflammation of the respiratory tract that every winter affects more than 100 million people in Europe, Japan and the United States of America, also being responsible for several thousand of excess deaths (data from the United States reveal between 20,000 to 40,000 excess deaths annually). The Mixovirus influenzae is the agent that causes influenza, commonly called flu. There are 3 types of influenza virus: A, B, C, and only types A and B are perceived to be clinically relevant in humans. Due to the segmented nature of its genetic material, the influenza virus is highly mutagenic, causing frequent insertion of new antigenic strains into the community, against which the population presents no immunity. Presently, there are few options for the control of influenza and annual immunization is the most effective means to prevent disease and its complications. In Brazil, according to data collected by the VigiGripe's Project - linked to the Federal University of Sao Paulo -, circulation of the influenza virus also has a seasonal pattern, with peak activity occurring between May and September. Yearly vaccination is, therefore, best indicated on March and April. Currently, there are four medications available for the treatment of influenza viruses: amantadine and rimantadine, and two second generation pharmaceutical products, the neuraminidase inhibitors, oseltamivir and zanamivir. The latter two drugs have set the stage for a new approach to the management and control of influenza infections.

154489 downloads Cited 20 times in SciELO

12.

Occurrence of influenza B/Hong Kong-Like strains in Brazil, during 2002
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Paiva, Terezinha Maria de

; Ishida, Maria Akiko

; Gonçalves, Maria Gisele

; Benega, Margareth Aparecida

; Souza, Maria Candida Oliveira de

; Cruz, Aurea Silveira

Revista do Instituto de Medicina Tropical de São Paulo

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jan 2003, Volume 45 N. 1 Pages 51 - 52

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A vigilância do vírus da influenza, no periodo de janeiro a outubro de 2002, detectou a circulação da estirpe B/Hong Kong/330/2001 nas regiões Sudeste e Centro Oeste do país. Esta estirpe é uma variante do vírus da influenza B/Victoria/02/88 a qual teve sua circulação circunscrita no continente asiático por volta de 1991. Variantes da estirpe B/Hong Kong/330/2001 (B/Hong Kong/1351/2002) e da estirpe B/Sichuan/379/99 (B/Shizuoka/5/2001) também foram detectadas. A porcentagem de vírus caracterizados durante a epidemia foi de 34,88%, 15,12%, 15,12%, 4,65%, 15,12%, 13,95%, nas faixas etárias de 0-4, 5-10, 11-15, 16-20, 21-30, 31-50, respectivamente. Observou-se maior incidência da doença entre os indivíduos menores de 4 anos. Atribuiu-se a ausência de notificação nos indivíduos maiores de 60 anos ao contato prévio, desta faixa etária, com o vírus na década de 80. No mesmo período, detectou-se a circulação do vírus da influenza A/Panamá/2007/99-like (H3N2), por outro lado evidenciou-se a ausência da circulação da estirpe A(H1N1) até a presente data.

Through the influenza virus surveillance from January to October 2002, influenza B/Hong Kong-like strains circulating in the Southeast and Centre East regions of Brazil have been demonstrated. This strain is a variant from B/Victoria/02/88 whose since 1991 and until recently have been isolated relatively infrequently and have been limited to South-Eastern Asia. A total of 510 respiratory secretions were collected from patients 0 to 60 years of age, with acute respiratory illness, living in the Southeast and Centre East regions of Brazil, of which 86 (17.13%) were positive for influenza virus. Among them 12 (13.95%) were characterized as B/Hong Kong/330/2001; 3 (3.49%) as B/Hong Kong/1351/2002 a variant from B/Hong Kong/330/2001; 1 (1.16%) as B/Sichuan/379/99; 1 (1.16%) as B/Shizuoka/5/2001, until now. The percentages of cases notified during the surveillance period were 34.88%, 15.12%, 15.12%, 4.65%, 15.12%, 13.95%, in the age groups of 0-4, 5-10, 11-15, 16-20, 21-30, 31-50, respectively. The highest proportion of isolates was observed among children younger than 4 years but serious morbidity and mortality has not been observed among people older than 65 years, although B influenza virus component for vaccination campaign 2002 was B/Sichuan/379/99 strain. This was probably due to the elderly protection acquired against B/Victoria/02/88. In addition, in influenza A/Panama/2007/99-like (H3N2) strains 22 (25.58%) were also detected, but influenza A(H1N1) has not been detected yet.

1989 downloads Cited 3 times in SciELO

13.

Outbreak of influenza type A (H1N1) in Iporanga, São Paulo State, Brazil
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PAIVA, Terezinha Maria de

; ISHIDA, Maria Akiko

; HANASHIRO, Kazue Aparecida Yamamoto

; SCOLARO, Regina Maria

; GONÇALVES, Maria Gisele

; BENEGA, Margareth Aparecida

; OLIVEIRA, Meire Aparecida S.

; CRUZ, Aurea Silveira

; TAKIMOTO, Sueko

Revista do Instituto de Medicina Tropical de São Paulo

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dec 2001, Volume 43 N. 6 Pages 311 - 315

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Durante os meses de junho e julho de 1999, foram notificados 324 casos de doença respiratória aguda no Município de Iporanga-SP. O isolamento do vírus da influenza do tipo A/Bayern/07/95 (H1N1) e a conversão sorológica para a estirpe viral (H1N1) foram de 57,1% e 100%, respectivamente. A porcentagem de casos com diagnóstico clínico notificados durante a epidemia foi de 28,4%, 29,0%, 20,7%, 6,2% e 15,7%, nas faixas etárias de 0-4, 5-9, 10-14, 15-19 anos e indivíduos acima de 20 anos de idade, respectivamente. Observou-se maior incidência da doença entre os indivíduos menores de 14 anos. Atribui-se a ausência de notificação de casos em indivíduos maiores de 65 anos à campanha de vacinação, na população idosa de Iporanga, que em 1999 atingiu 72,4%. O virus isolado é genética e antigenicamente semelhante à estirpe A/Beijing/262/95 (H1N1), o componente H1 da vacina de 1999. Vacinas contendo a estirpe A/Beijing/262/95 (H1N1) estimularam, após imunização, anticorpos inibidores da hemaglutinação, os quais foram equivalentes em freqüência e título para ambas as estirpes: A/Bayern/07/95 e A/Beijing/262/95.

From June to July 1999 an outbreak of acute respiratory illness occurred in the town of Iporanga. Out of a total of 4,837 inhabitants, 324 cases were notified to the Regional Surveillance Service. Influenza virus was isolated from 57.1% of the collected samples and 100% seroconversion to influenza A (H1N1) was obtained in 20 paired sera tested. The isolates were related to the A/Bayern/07/95 strain (H1N1). The percentages of cases notified during the outbreak were 28.4%, 29.0%, 20.7%, 6.2% and 15.7% in the age groups of 0-4, 5-9, 10-14, 15-19 and older than 20 years, respectively. The highest proportion of positives was observed among children younger than 14 years and no cases were notified in people older than 65 years, none of whom had been recently vaccinated against influenza. These findings suggest a significant vaccine protection against A/Bayern/7/95, the H1 component included in the 1997-98 influenza vaccine for elderly people. This viral strain is antigenically and genetically related to A/Beijing/262/95, the H1 component of the 1999 vaccine. Vaccines containing A/Beijing/262/95 (H1N1) stimulated post-immunization hemagglutination inhibition antibodies equivalent in frequency and titre to both A/Beijing/262/95-like and A/Bayern/7/95-like viruses. Thus, this investigation demonstrates the effectiveness of vaccination against influenza virus in the elderly.

2360 downloads Cited 2 times in SciELO

14.

Detecção de soroalbuminas e imunoglobulinas no leite bovino como indicadores de mastite subclínica
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Amaral, Jackson Barros do

; Charles, Terezinha Padilha

; Brito, Maria Aparecida Vasconcelos Paiva e

Ciência Rural

Journal Metrics
- Sobre o periódico
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1995, Volume 25 N. 1 Pages 93 - 97

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Com o objetivo de verificar a utilidade da detecção de soroalbumina (BSA) e imunoglobulinas (Ig) no leite como diagnóstico da mastite subclínica, avaliou-se a relação entre a concentração dessas proteínas, o exame microbiológico e o teste "California Mastitis Test" (CMT). Amostras de leite de 172 quartos mamários previamente examinadas pelo CMT foram colhidas e levadas imediatamente ao laboratório para exames bacteriológicos. Uma alíquota de cada amostra foi congelada a -20°C e posteriomente testada para a presença de BSA e Ig, por meio da técnica de imunodifusão radial simples. Das amostras testadas, 111 apresentaram níveis fisiológicos de BSA (< 0,2mg/ml) e de Ig (< 0,5 mg/ml), 22 apresentaram níveis elevados de ambas proteínas e 39 apresentaram níveis elevados de Ig, mas níveis fisiológicos de BSA. Concentrações de BSA e Ig acima dos limites fisiológicos foram observadas mais frequentemente em amostras onde o CMT apresentou reação de maior severidade (+++) e naquelas onde foram isoladas Streptococcus spp. e Staphylococcus aureus. Entre as 88 amostras positivas no exame bacteriológico, níveis de BSA e Ig acima do limite fisiológico foram observados em 10 e 35, respectivamente. Em amostras onde não se isolou bactérias (84 das 172 examinadas), níveis fisiológicos de BSA e Ig foram observados em 72 e 58 delas, respectivamente. A detecção de BSA e Ig nas amostras de leite forneceu informações adicionais sobre o grau de lesão tecidual, mas não pode ser considerada como um indicador sensível para detecção precoce da reação inflamatória.

This work aimed to evaluate the feasibility of milk serum albumin (BSA) and immunoglobulin (Ig) detection for the diagnosis of subclinical mastitis. Bacteriological examinations and concentrations of BSA and Ig were determined in 172 milk samples. The samples were also examined by the California Mastitis Test (CMT). Immediately after collection, the samples were taken to the laboratory and bacteriologically examined. An aliquot of each sample was kept at -20°C and later, concentrations of BSA and Ig were determined by radial immunodiffusion. A total of 111 milk samples had physiological levels of BSA (< 0.2 mg/ml) and Ig (< 0.5mg/ml). Twenty-two samples had higher levels of both proteins and 39 samples had higher levels of Ig and physiological levels of BSA. Concentrations of BSA and Ig above the physiological levels were more frequently observed in quarters samples with severo (+++) CMT reaction and udder infection caused by Streptococcus spp and Staphylococcus aureus. In bacteriologically positive quarters (88 out of 172), increased levels of BSA and Ig were found in 10 and 35 samples, respectively. In the bacteriologically negativo quartors (84 out of 172), physiological levels of BSA and Ig were found in 72 and 58 samples, respectively. Although BSA and Ig measurements in milk samples gave further information about udder tissue damage, they can not be used as a sensitive parameter to detect early inflammatory reaction.

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15.

Comparative study of adenoviruses with monoclonal antibodies
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Paiva, Terezinha Maria de

; Takimoto, Sueko

; Ishida, María Akíko

; Souza, María Candida Oliveira de

; Ishimaru, Tuneo

; Neumann, Jorge

; Kalil, Jorge

Revista do Instituto de Medicina Tropical de São Paulo

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feb 1992, Volume 34 N. 1 Pages 19 - 26

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O estudo relata a obtenção de anticorpos monoclonais para o adenovirus tipo (espécie) 4. A especificidade dos anticorpos monoclonais, selecionados nesse experimento, foi determinada testando-os frente às diferentes espécies de adenovírus: 1, 2, 5 (subgênero C), 4 (subgênero E), 7 e 16 (subgênero B) e 9 (subgênero D), pelas técnicas de neutralização em cultura de células, imunofluorescência e ensaio imunoenzimático (ELISA). Os resultados demonstram a obtenção de anticorpos monoclonais que reagiram de maneira específica para o adenovírus 4 (1CIII e 3DIII) e anticorpo monoclonal apresentando reação cruzada com as espécies de adenovírus 4 e 7 (2HIII).

The obtainment of monoclonal antibodies for adenovirus species 4(Ad4) is described.The specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA), in the presence of the following species of human adenovirus: 1, 2, 5 (subgenus C), 4 (subgenus E), 7 and 16 (subgenus B) and 9 (subgenus D). Two monoclonal antibodies species specific to adenovirus 4 (1CIII and 3DIII) and one monoclonal antibody that cross reacted with adenovirus species 4 and 7 (2HIII) were obtained.

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