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ABSTRACT Objective. To provide a comprehensive overview of geographical patterns (2001–2010) and time trends (1993–2012) of cancer incidence in children aged 0–19 years in Latin America and the Caribbean (LAC) and interpret the findings in the context of global patterns. Methods. Geographical variations in 2001–2010 and incidence trends over 1993–2012 in the population of LAC younger than 20 years were described using the database of the third volume of the International Incidence of Childhood Cancer study containing comparable data. Age-specific incidence per million person-years (ASR) was calculated for population subgroups and age-standardized (WSR) using the world standard population. Results. Overall, 36 744 unique cases were included in this study. In 2001–2010 the overall WSR in age 0–14 years was 132.6. The most frequent were leukemia (WSR 48.7), central nervous system neoplasms (WSR 23.0), and lymphoma (WSR 16.6). The overall ASR in age group 15–19 years was 152.3 with lymphoma ranking first (ASR 30.2). Incidence was higher in males than in females, and higher in South America than in Central America and the Caribbean. Compared with global data LAC incidence was lower overall, except for leukemia and lymphoma at age 0–14 years and the other and unspecified tumors at any age. Overall incidence at age 0–19 years increased by 1.0% per year (95% CI [0.6, 1.3]) over 1993–2012. The included registries covered 16% of population aged 0–14 years and 10% of population aged 15–19 years. Conclusions. The observed patterns provide a baseline to assess the status and evolution of childhood cancer occurrence in the region. Extended and sustained support of cancer registration is required to improve representativeness and timeliness of data for childhood cancer control in LAC.

RESUMO Objetivo. Apresentar uma visão abrangente dos padrões geográficos (2001 a 2010) e das tendências temporais (1993 a 2012) da incidência de câncer em crianças e jovens de 0 a 19 anos na América Latina e no Caribe (ALC) e interpretar os resultados no contexto de padrões mundiais. Métodos. Foram descritas variações geográficas de 2001 a 2010 e tendências de incidência de 1993 a 2012 na população com menos de 20 anos da ALC usando informações comparáveis da base de dados do terceiro volume do estudo International Incidence of Childhood Cancer. Foram calculadas taxas de incidência específica por idade por milhão de pessoas-ano (ASR, na sigla em inglês) para subgrupos populacionais e taxas padronizadas por idade usando a população padrão mundial (WSR, na sigla em inglês). Resultados. No total, foram incluídos 36 744 casos únicos. No período de 2001 a 2010, a WSR para todos os tumores combinados na faixa etária de 0 a 14 anos foi de 132,6. Os diagnósticos mais frequentes foram leucemia (WSR de 48,7), neoplasias do sistema nervoso central (WSR de 23,0) e linfoma (WSR de 16,6). A ASR para todos os tumores combinados na faixa etária de 15 a 19 anos foi de 152,3, e a maior taxa foi a de linfoma (ASR de 30,2). A incidência foi maior no sexo masculino do que no sexo feminino e maior na América do Sul do que na América Central e no Caribe. De modo geral, em comparação com as estimativas mundiais, a incidência na ALC foi menor, exceto para leucemia e linfoma entre 0 e 14 anos e para outros tumores e tumores não especificados em qualquer idade. A taxa de incidência na faixa etária de 0 a 19 anos aumentou em 1,0% ao ano (IC de 95% [0,6, 1,3]) entre 1993 e 2012. Os registros incluídos cobriam 16% da população de 0 a 14 anos e 10% da população de 15 a 19 anos. Conclusões. Os padrões observados servem de referência para avaliar o status e a evolução da ocorrência de câncer infantil na região. É necessário garantir um apoio ampliado e consistente aos registros de câncer para aprimorar a representatividade e a disponibilidade das informações em tempo adequado para o controle do câncer infantil na ALC.

https://doi.org/10.26633/rpsp.2024.11

Efectividad de la electroacupuntura y moxibustión en el tratamiento del dolor postquirúrgico del injerto por plastia del ligamento cruzado anterior con remodelación meniscal. Estudio de caso UNEVT 2022
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Alonso, JA

; Nava, KB

; Avendaño, RA

; Ortiz, MC

; Torres, LL

; Patiño, AC

Revista de la Sociedad Española del Dolor

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jun 2022, Volume 29 N. 3 Pages 192 - 195

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Objective: To analyze the response of post-surgical pain to the application of electroacupuncture and moxibustion using the IKDC scale. Methods: Quasi-experimental study in the integral clinic of the Universidad Estatal del Valle de Toluca, the treatment consisted of the consented application of ten sessions of electroacupuncture and moxibustion distributed in two weekly sessions of 30 minutes for the monitoring of postoperative pain in anterior cruciate ligament plasty. Results: 100 % pain reduction (VAS 0/10) was obtained from the sixth session (21 days), 100 % recovery of the surgical wounds was observed, edema was reduced to 75 % (Godet sign 1+/4+) and mobility was increased to 59% IKDC (47.4/80 total points). Conclusions: The effectiveness of therapy with electroacupuncture (1) and moxibustion (2,3) in the treatment of post-surgical pain was evidenced by means of the IKDC (4) scale at the end of the ten sessions, in addition to accelerated healing, decreased edema and increased joint mobility in less time, being a therapeutic option in analgesia and recovery from surgical procedures as long as it is practiced according to the scientific, clinical bases.

RESUMEN Objetivo: Analizar la respuesta del dolor postquirúrgico ante la aplicación de electroacupuntura y moxibustión mediante la escala IKDC. Método: Estudio cuasiexperimental en la clínica integral de la Universidad Estatal del Valle de Toluca. el tratamiento consistió en la aplicación consentida de diez sesiones de electroacupuntura y moxibustión distribuidas en dos sesiones semanales de 30 minutos para el monitoreo del dolor postquirúrgico en plastia del ligamento cruzado anterior. Resultados: Se obtuvo disminución del dolor al 100 % (EVA 0/10) a partir de la sexta sesión (21 días) observó recuperación de las heridas quirúrgicas al 100 %, disminución del edema al 75 % (signo Godet 1+/4+) y aumento de la movilidad al 59 % IKDC (47,4/80 puntos totales). Conclusiones: Se evidenció la efectividad de la terapia con electroacupuntura (1) y moxibustión (2,3) en el tratamiento del dolor postquirúrgico por medio de la escala IKDC (4) al concluir las diez sesiones, además de cicatrización acelerada, disminución del edema y aumento en la movilidad articular en menor tiempo, siendo una opción terapéutica en la analgesia y recuperación de procedimientos quirúrgicos siempre que se practique conforme a las bases científicas, clínicas.

https://doi.org/10.20986/resed.2022.4009/2022

Creación del Comité de Ejercicio y Enfermedad Renal Crónica de la Sociedad Chilena de Nefrología
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Müller-Ortiz, Hans

; Moscoso-Aguayo, Paula

; González-Burboa, Alexis

; Vera-Calzaretta, Aldo

; Opazo-Ríos, Lucas

; Basáez-Fernandez, Ailyn

; Mc Kenzie-Atala, Lilian

Revista médica de Chile

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mar 2021, Volume 149 N. 3 Pages 478 - 479

Abstract: Es | Text: Es | PDF: Es

https://doi.org/10.4067/s0034-98872021000300478 175 downloads

Comparación de la composición y de la actividad antioxidante del aceite esencial de manzanilla (Matricaria chamomilla L.) obtenido mediante extracción con fluidos supercríticos y otras técnicas verdes
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Melo-Guerrero, María Camila

; Ortiz-Jurado, Daniel Esteban

; Hurtado-Benavides, Andrés Mauricio

Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales

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sep 2020, Volume 44 N. 172 Pages 845 - 856

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Se comparó la composición química y la actividad antioxidante del aceite esencial de manzanilla (Matricaria chamomilla L.) obtenido mediante extracción con fluidos supercríticos (supercritical fluid extraction, SFE) y otras técnicas de extracción verdes (arrastre con vapor, AV; hidrodestilación, HD; destilación por arrastre con vapor y extracción simultánea con solvente orgánico, DES, y maceración, MC). Se optimizó el proceso de extracción evaluando el efecto de la temperatura (T) (35-65 °C) y la presión (P) (90-210 bar) en el rendimiento de la extracción (% p/p) y se determinó que una temperatura de 65 °C y una presión de 210 bar eran las mejores condiciones de extracción (0,87 % p/p). La composición química se analizó por cromatografía de gases acoplada a espectrometría de masas (GC-MS) y se establecieron los constituyentes con base en sus patrones de fragmentación y el índice de retención de Kovats. La actividad antioxidante se evaluó mediante el método ABTS. Los componentes principales fueron enindicicloeter, P-farneseno y óxido de bisabolol A. Los resultados de los análisis de GC-MS indicaron que la composición y concentración del aceite esencial cambiaron al emplear diferentes métodos de extracción, por ejemplo, en los aceites extraídos por MC el componente mayoritario fue el enindicicloeter, en tanto que en los extraídos por HD fue el β-farneseno y con DES fue el óxido de bisabolol A. El aceite obtenido por AV (27,56 μmol de trolox/ml de aceite) presentó una mayor actividad antioxidante en comparación con los otros métodos de extracción, incluso comparado con el SFE (15,25 μmol de trolox/ml de aceite).

Abstract The chemical composition and antioxidant activity of the chamomile essential oil (Matricaria chamomilla L.) obtained by supercritical fluids extraction (SFE) and other green techniques (steam stripping, AV; hydrodistillation, HD; steam-dragging distillation with simultaneous steam-extraction using an organic solvent, DES, and maceration (MC). We optimized the extraction process with supercritical fluids by checking the effect of temperature (35-65°C) and pressure (90-210 bar) on the extraction yield (% w/w) and we determined that the best extraction conditions (0.87% w / w) were at 65°C and 210 bar. We analyzed the chemical composition by gas chromatography-mass spectrometry (GC-MS) and identified the constituents based on their fragmentation patterns and Kovat's index. We evaluated the antioxidant activity using the ABTS method. The main components were en-yn-dicycloeter, β-farnesene, and bisabolol oxide A. The results of the GC-MS analyses indicate that when using different extraction methods the composition and concentration of the essential oil changes, for example, the oils extracted by MC had en-yn-dicycloeter is their main component while for those extracted by HD it was β-farnesene, and by DES ii was bisabolol oxide A. The oil obtained by AV (27.56 μmol trolox/ml of oil) had a higher antioxidant activity compared to the other extraction methods, even when compared with SFE (15.25 μmol trolox/ml of oil).

https://doi.org/10.18257/raccefyn.862 402 downloads

Enraizado de brotes in vitro y aclimatación de plantas de Agave potatorum Zucc.
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Bautista-Castellanos, Ariadna Ivón

; Enríquez-del Valle, José Raymundo

; Velasco-Velasco, Vicente Arturo

; Rodríguez-Ortiz, Gerardo

Ecosistemas y recursos agropecuarios

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2020, Volume 7 N. 3 elocation e2618

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT. Agave potatorum Zucc., is a wild or cultivated monocotyledonous species, used to make mescal, and due to its growing demand it is collected excessively. The objective was to know the effect of the concentration of mineral salts, indole-acetic acid (IAA) and indole-3-butyric acid (IBA) in the culture medium, Mc, on the in vitro rooting of shoots, the acclimatization and growth of the micropropagated plants that received different doses of fertilization in the nursery. The work consisted of two stages: 1) shoots grown for 66 days in Mc with different concentration of inorganic salts MS (75 or 100%), auxin (IAA or IBA) alone or combined in concentrations of 0, 0.1, or 0.5 mg L-1. In stage 2, groups of plants originating from rooted shoots were transferred to trays with 33% peat-66% perlite substrate, and were fertirrigated for 90 days with different doses of nutrient solution, SN (25, 50, or 100%) of the Steiner formulation. Between 94 and 96% of the shoots formed roots in Mc with 0.1 to 0.5 mg L-1 of IBA, after 13 to 37 days of incubation, the better response than the shoots in Mc without auxins, or with IAA. When the plants were 90 days in the nursery, from 87 to 90% were adapted. The plants fertirrigated with SN-50% developed an average of 8.9 leaves, with a máximum length of 8.4 cm, the stem of 10.27 mm in diameter, fresh weight of 4.15 g and dryweight of the plant of 0.31 g.

RESUMEN. Agave potatorum Zucc. es una especie monocotiledónea silvestre o cultivada, usada para elaborar mezcal, y por su creciente demanda es colectada de forma excesiva. El objetivo fue conocer el efecto de la concentración de las sales minerales, el ácido indol-acético (AIA) y ácido indol-3-butírico (AIB) en el medio de cultivo, Mc, sobre el enraizado de brotes in vitro, la aclimatación y crecimiento de las plantas micropropagadas que en vivero recibieron diferentes dosis de fertilización. El trabajo consistió de dos etapas: 1) brotes cultivados durante 66 días en Mc con concentración diferente de sales inorgánicas MS (75 o 100%), auxina (AIA o AIB) solas o combinadas en concentraciones de 0, 0.1, o 0.5 mg L-1. En la etapa 2, grupos de plantas originadas de brotes enraizados se transfirieron a charolas con sustrato 33% turba-66% perlita, y se fertirrigaron durante 90 días con dosis diferente de solución nutritiva, SN (25, 50, o 100%) de la formulación Steiner. Entre 94 y 96% de los brotes formaron raíces en Mc con 0.1 a 0.5 mg L-1 de AIB, transcurridos 13 a 37 días de incubación, siendo la mejor respuesta que los brotes en Mc sin auxinas, o con AIA. Cuando las plantas estuvieron 90 días en vivero, del 87 a 90% fue adaptada. Las plantas fertirrigadas con SN-50% desarrollaron en promedio 8.9 hojas, con largo máximo de 8.4 cm, el tallo de 10.27 mm de diámetro, peso fresco de 4.15 g y peso seco de la planta de 0.31 g.

https://doi.org/10.19136/era.a7n3.2618 70 downloads

Vacunación neumocócica conjugada en adultos. Recomendaciones de las Sociedades Médicas en México
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Mejía-Ávila, ME

; Ávila-Fematt, FMG

; Aguilar-Navarro, SG

; Alatorre-Alexander, JA

; Alcocer-Díaz Barreiro, LA

; Báez-Saldaña, R

; Buendía-Roldán, I

; Carrillo-González, PA

; Cornejo-Juárez, P

; Dávila-Valero, JC

; Donis-Hernández, JJ

; Franco-Cendejas, R

; García-Figueroa, JL

; Guerrero-Almeida, MC

; Gutiérrez-Ureña, SR

; Hernández-Núñez, E

; López-Enríquez, CC

; Pavia-Ruz, N

; Pedraza-Chávez, J

; Quintero-Beuló, G

; Regalado-Pineda, J

; Rodríguez-García, JA

; Salazar-Lezama, MA

; Sánchez-Mijangos, JH

; Sánchez-Ríos, CP

; Solache-Ortiz, G

; Torres-Gutiérrez, JL

; Vázquez-Cortés, JJ

; Vilar-Compte, D

; Wong-Chew, RM

; Zúñiga-Gil, CH

Neumología y cirugía de tórax

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jun 2019, Volume 78 N. 2 Pages 152 - 173

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract: Community-acquired pneumonia (CAP) is a frequent and potentially serious infection associated with high morbidity and mortality, particularly in adults and vulnerable groups, despite research and information and the development of new antimicrobial agents. This document represents the effort of a group of experts to disseminate knowledge about the prevention of CAP through pneumococcal conjugate vaccination in adults, especially in patients with comorbidities such as heart, lung and rheumatic diseases, diabetes mellitus, cancer and human immunodeficiency virus infection (HIV), among others. The objective is to provide recommendations on pneumococcal conjugate vaccination to physicians who treat adult patients with comorbidities such as those mentioned above.

Resumen: La neumonía adquirida en la comunidad (NAC) es una infección frecuente, potencialmente grave que se asocia con elevada morbilidad y mortalidad, particularmente en adultos y en grupos vulnerables, a pesar de la investigación e información al respecto y del desarrollo de nuevos agentes antimicrobianos. El presente documento representa el esfuerzo de un grupo de expertos para divulgar el conocimiento sobre la prevención de la NAC a través de la vacunación neumocócica conjugada en adultos, especialmente en aquellos pacientes con comorbilidades como cardiopatías, neumopatías, diabetes mellitus, enfermedades reumáticas, cáncer e infección por el virus de la inmunodeficiencia humana (VIH), entre otras. El objetivo de este documento es dar recomendaciones sobre la vacunación neumocócica conjugada a los médicos que atienden pacientes adultos con comorbilidades como las arriba mencionadas.

1743 downloads

Prevalencia de sífilis y características del comportamiento de los jóvenes indígenas del Paraguay, 2016
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Ortiz, Analía

; Estigarribia, Gladys

; Aguilar, Gloria

; Espinosa Miranda, Angélica

; Farland, William Mc

; Rios-González, Carlos Miguel

; Kawabata, Anibal

; Muñoz, Sergio

; Samudio, Tania

; López, Gladys

; Rodriguez, Aldo

Memorias del Instituto de Investigaciones en Ciencias de la Salud

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dec 2018, Volume 16 N. 3 Pages 51 - 57

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT The objective was to determine the prevalence of syphilis and behavioral characteristics of indigenous youth of Paraguay in 2016. An observational, descriptive, cross-sectional study was carried out with multistage stratified probabilistic sampling. Indigenous youth aged 15 to 18 from five linguistic families living in Paraguay were included. A structured questionnaire was used and a rapid treponemal test was used for the syphilis screening. The results are expressed in measures of central tendency, dispersion and proportions. Five hundred forty six young people from 15 to 18 years old entered the study, 67.03% was female. There were 36 cases of syphilis detected, resulting in a prevalence of 6.6% [95% CI: 4.7-9.0]. Young people who consumed alcohol in the last sexual intercourse 20.6% (14/68) (p = 0.000), transactional sex 40% (2/5) (p = 0.010) and non-use of condom 20.6% (14/68) (p = 0.000) had higher prevalence of syphilis. We found a high prevalence of syphilis in the indigenous juvenile population related to drinking alcohol during the last relationship, practice of transactional sex and the non-use of condoms. Specific strategies should be implemented with an intercultural approach appropriate for a young population, aimed at reducing risk practices and promoting the use of condoms.

RESUMEN El objetivo fue determinar la prevalencia de sífilis y características de comportamiento de los jóvenes indígenas del Paraguay en el 2016. Se realizó un estudio observacional, descriptivo de corte transversal con muestro probabilístico estratificado bietapico, que incluyó a jóvenes indígenas de 15 a 18 años de cinco familias lingüísticas de Paraguay. Se utilizó un cuestionario estructurado y para el tamizaje de sífilis se utilizó una test rápido treponémico y para confirmar los resultados reactivos se realizó VDRL, considerando como resultado positivo si el test rápido era positivo + VDRL positivo a una dilución de 1:4 o mayor y si la VDRL era menor a 1:4 con TPHA positivo. Los resultados se expresan como medidas de tendencia central, dispersión y proporciones. Ingresaron al estudio 546 jóvenes de 15 a 18 años, el 67,03% era del sexo femenino. Se identificaron 36 casos de sífilis, que representa una prevalencia de 6,6% (IC95%: 4,7-9,0). Mayor prevalencia de sífilis se observó en los jóvenes que consumieron alcohol en la última relación sexual 20,6% (14/68) (p<0,001), sexo transaccional 40% (2/5) (p=0,010) y no utilización de condón 20,6% (14/68) (p<0,001). Se encontró una alta prevalencia de sífilis en la población juvenil indígena, relacionada al consumo de alcohol en la última relación, práctica de sexo transaccional y no utilización de preservativos. Se recomienda la implementación de estrategias específicas con abordaje intercultural apropiadas para una población joven, orientadas a disminuir las prácticas de riesgo y promocionar la utilización de preservativos.

https://doi.org/10.18004/mem.iics/1812-9528/2018.016(03)51-057 682 downloads

Estudio in vitro del Efecto Antibacteriano de la Oleorresina de Copaifera reticulata y el Aceite Esencial de Origanum majoricum Frente a Streptococcus mutans y Enterococcus Faecalis Bacterias de Importancia en Patologías Orales
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Moromi-Nakata, Hilda

; Ramos-Perfecto, Donald

; Villavicencio-Gastelumendi, Jorge

; Martínez-Cadillo, Elba

; Mendoza-Rojas, Alejandro

; Chavez-Alvarado, Edith

; Ortiz-Fernández, Lita

; Quispe-Salcedo, Ángela

International journal of odontostomatology

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dec 2018, Volume 12 N. 4 Pages 355 - 361

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT: The objective of the study was to determine the in vitro antibacterial effect of the oleoresin of Copaifera reticulata (C. reticulata) "copaiba" and of the essential oil of Oreganum majoricum (O. majoricum) "oregano" against Streptococcus mutans (S. mutans) and Enterococcus faecalis (E. faecalis). Sensitivity tests were developed by first activating the bacteria strains to be confronted. The oleoresin of copaiba was diluted with dimethylsulphoside (DMSO), obtaining final concentrations to be tested of 100 %, 50 %, 25 %, and 12.5 %. In relation to the essential oil of oregano, it was only 100 % tested. For the disk agar diffusion test, 100 mL of each bacterial strain was taken at a turbidity of 0.5 of Mc Farlam, to be planted by dissecting trypticase soy agar plates, and then placing the disks equidistantly loaded with the different concentrations of natural products; 0.12 % chlorhexidine was used as a positive control and DMSO as negative control. The plates were incubated by the candle method in extinction at 37 °C, for a period of 24 hours, after which time the inhibition halos were read. The results obtained by the copaiba, determined an antibacterial effect in its four concentrations, being the biggest halos of inhibition at the concentration of 100 %, copaiba genus higher average halos for S. mutans of 30.00 ± 0.00 mm and for E. faecalis of 8.3 ± 0.50 mm. In the case of oregano, haloes are produced at a concentration of 100 % with an average of 25.3 ± 0.96 mm for S. mutans and for E. faecalis 9.5 ± 1.29 mm. It is concluded from the study that both copaiba and oregano present an antibacterial effect for both bacteria, being its greater antibacterial effect for both natural products on S. mutans.

RESUMEN: El objetivo del estudio fue determinar el efecto antibacteriano in vitro de la oleorresina de Copaifera reticulata (C. reticulata) “copaiba” y del aceite esencial de Oreganum majoricum (O. majoricum) “orégano” frente a Streptococcus mutans (S. mutans) y Enterococcus faecalis (E. faecalis). Se desarrollaron pruebas de sensibilidad activando primero las cepas bacterias a enfrentar. La oleorresina de copaiba fue diluida con dimetilsulfósido (DMSO), obteniéndose al final concentraciones a probar de 100 %, 50 %, 25 %, y 12,5 %. En relación al aceite esencial de orégano este se probó solamente al 100 %. Para la prueba de difusión en agar con discos, se tomaron inóculos 100 µL de cada cepa bacteriana a una turbidez de 0,5 de Mc Farlam, para ser sembrados por diseminación en placas de tripticasa soya agar, para luego colocar los discos de forma equidistante cargados con las diferentes concentraciones de los productos naturales, se utilizaron como control positivo a la clorhexidina al 0,12 % y al DMSO como control negativo. Se incubaron las placas por el método de la vela en extinción a 37 °C, por un periodo de 24 horas, pasado el tiempo se realizó la lectura de los halos de inhibición. Los resultados obtenidos por la copaiba, determinaron un efecto antibacteriano en sus cuatro concentraciones, siendo los mayores halos de inhibición a la concentración del 100 %, copaiba genero mayores halos promedios para S, mutans de 30,00 ± 0,00 mm y para E. faecalis de 8,3 ± 0,50 mm. Para el caso del orégano se producen halos a la concentración del 100 % con un promedio de 25,3 ± 0,96 mm para S. mutans y para E. faecalis de 9,5 ± 1,29 mm. Se concluye del estudio que tanto copaiba como el orégano presentan un efecto antibacteriano para ambas bacterias, siendo su mayor efecto antibacteriano para ambos productos naturales sobre S. mutans.

1846 downloads

Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots
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Ríos-Ramírez, Suzel C.

; Enríquez-delValle, José R.

; Rodríguez-Ortiz, Gerardo

; Ruíz-Luna, Judith

Ciencia e investigación agraria

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dec 2017, Volume 44 N. 3 Pages 285 - 294

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Today, a micropropagation method for Agave angustifolia exists, but for the multiplication of propagules, more information is needed on the diverse components of the culture medium, cytokinins and auxins and their effect on organogenetic response. The objective of this study was to evaluate dosages of benzylaminopurine and indol-3-acetic acid (IAA) in a culture medium and their effect on the formation of new adventitious shoots (organogenesis). The explants used were clusters of shoots on a common base of A. angustifolia stem tissue. Clusters of three to four shoots were established in different variants of a Murashige and Skoog culture medium that had different concentrations of benzylaminopurine (BAP; 0.5, 1, 2, and 4 mg L−1) and IAA) (0.1, 0.3, and 1 mg L−1). After 16 wk, it was found that a higher proliferation of shoots was positively related to the concentration of growth regulators. Explants formed a greater quantity of shoots in a culture medium with an increase in BAP of up to 4 mg L−1 and in IAA of up to 1 mg L−1, and there was a synergistic effect with BAP to induce the formation of the greatest number of shoots.

Resumen Actualmente existe una metodología para micropropagar a Agave angustifolia, y en la multiplicación de propágulos, se requiere mayor información sobre los diversos componentes del medio de cultivo, citocininas y auxinas en su efecto sobre la respuesta de organogénesis. Por lo que el objetivo fue evaluar las dosis de bencilaminopurina y ácido indol-3-acético (AIA) en el medio de cultivo, en su efecto para la formación de nuevos brotes adventicios (organogénesis). Se tomaron explantes que fueron racimos de brotes en una base común de tejidos de tallos de A. angustifolia. Se establecieron racimos de 3 a 4 brotes de A. angustifolia en diversas variantes de medio de cultivo MS que tenían diferentes concentraciones de bencilaminopurina BAP (0.5 1, 2, 4 mg L-1) y AIA (0.1, 0.3, 1 mg L-1). Transcurridas 16 semanas, se obtuvo que la mayor proliferación de brotes fue en relación positiva con la concentración de reguladores de crecimiento, en explantes establecidos en medio de cultivo con 4 mg L-1 BA + 1 mg L-1 AIA y explantos en MC con 0.5 mg L-1 BA + 0.1 mg L-1 AIA se formaron en promedio 32.8 y 7.8 brotes, con 45 y 24 hojas, respectivamente.

https://doi.org/10.7764/rcia.v44i3.1810 810 downloads

10.

Molecular Diagnosis of Beak and Feather Disease in Native Brazilian Psittacines
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Araújo, AV

; Andery, DA

; Ferreira Jr., FC

; Ortiz, MC

; Marques, MVR

; Marin, SY

; Vilela, DAR

; Resende, JS

; Resende, M

; Donatti, RV

; Martins, NRS

Brazilian Journal of Poultry Science

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dec 2015, Volume 17 N. 4 Pages 451 - 458

Abstract: En | Text: En | PDF: En

ABSTRACT The incidence of the psittacine beak and feather disease virus (BFDV) was investigated in Brazilian native parrots with normal feathering arriving at rescue and triage centers for wild animals (CETAS, IBAMA) in the state of Minas Gerais, Brazil. BFDV DNA was investigated by previously described PCR technique for the partial amplification of BFDV ORF-1 in DNA extracts from blood, cloacal swab or liver of psittacines. Some birds provided more than one sample. Nine species of psittacines were sampled between January 2009 and October 2010. Blood (n=46) or cloacal swab (n=128) samples were obtained from psittacines immediately upon arrival at the triage centers. Liver samples were collected from necropsied birds dead on arrival (n=167). All swab samples were negative, except for one Ara ararauna individual (n=3) which blood presented the BFDV DNA. On the other hand, 11 liver samples were positive for BFDV DNA, with a prevalence of 7.8% in Amazona aestiva (n=140). No BFDV DNA was detected in the liver of Amazona amazonica (n=11), A. vinacea (n=5), A. rhodochorytha (n=4), Anodorhynchus hyacinthinus (n=3), Ara ararauna, (n=3), Aratinga leucophtalma (n=2), Guarouba guarouba (n=1) and Pionus maximiliani (n=1). In most cases, alopecia was not associated with BFDV detection in liver, and liver histopathology was inconclusive. Although all cloacal swab samples were negative, a few psittacines (n=19) that died at CETAS-Belo Horizonte were retested, and 21% were detected as positive in liver. A group of psittacines (n=16) was clinically evaluated, and despite showing feather dystrophy, all birds were negative in the cloacal swabs, except for one, which blood sample was positive (A. ararauna). The obtained sequences of the BFDV strains BH 215 and BH 732 were deposited in the GenBank (JQ649409 and JQ649410). A 98% similarity with strain sequences described in Australia, Japan, and New Zealand was observed. It is possible that these strains arrived in Brazil through the legal and illegal trade of parrots. However, it was not possible to associate BFDV infection with the geographical origin of birds and no local marker was detected. The rates of detection, although similar to other studies, indicate the tendency of a high incidence of the disease, possibly associated with stress, and high bird density and wide transmission in captivity conditions.

https://doi.org/10.1590/1516-635X1704451-458 2441 downloads

11.

Health assessment of raptors in triage in Belo Horizonte, MG, Brazil
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Andery, D de A

; Ferreira Junior, FC

; Araújo, AV de

; Vilela, DA da R

; Marques, MVR

; Marin, SY

; Horta, RS

; Ortiz, MC

; Resende, JS de

; Martins, NR da S

Brazilian Journal of Poultry Science

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sep 2013, Volume 15 N. 3 Pages 247 - 256

Abstract: En | Text: En | PDF: En

Falconiformes (n=82), Strigiformes (n=84) and Cathartiformes (n=14) at a triage center (CETAS-Belo Horizonte, IBAMA, Brazil) were examined between 2008 and 2010 . No bird was reactive at hemagglutination-inhibition (HI) for antibodies against Mycoplasma gallisepticum (Mg). Two Caracara plancus (2/68) had HI titers (16-32) against Newcastle disease virus. No Chlamydophila psittaci DNA was detected in the liver (PCR; n=95). Blood smears (Giemsa; n=89) and spleen fragments (PCR; n=82) were 13.5% and 8.5% positive, respectively, for Haemoproteus only. Necropsy of Cathartiformes (n=10), Falconiformes (n=42) and Strigiformes (n=57) showed that trauma injuries were the main cause (63.3%) of admission and death, being fractures (38.5%) of the thoracic limbs (57.1%) the most frequent. Nematode (12.8%), cestode (1.8%), trematode (0.9%), and acanthocephalan (2.7%) parasite infections were relevant. Mites (Acari) were the most frequent (17.4%) external parasites, particularly Ornithonyssus sylviarum in Asio clamator and Amblyomma cajennense in Tyto alba. Chewing lice (10.1%) and Pseudolynchia spp. (9.2%) were also found. Histomonas spp. (6.4%) was found in the ceca of Bubo virginianus, Athene cunicularia, Tyto alba, and Asio clamator, but not in Falconiformes or Cathartiformes. Trichomonas spp. (oral cavity, pharynx and upper esophagus; 9.1%) was detected in Falconiformes and Strigiformes, but not in Cathartiformes. Trichomonas spp. were found in A. cunicularia, Asio clamator, Glaucidium brasilianum and Tyto alba (Strigiformes), and in Rupornis magnirostris, Milvago chimachima, Falco femoralis, Falco sparverius and Caracara plancus (Falconiformes). Coccidia (9.1%) (Sarcocystis spp., 6.4%) and mycosis were observed in most Tyto alba (70%). The evaluated Orders may not pose risks for commercial poultry production. Habitat loss and urban adaptation may be increasingly affecting raptors.

3787 downloads Cited 1 time in SciELO

12.

Mycobacterium avium subsp. Paratuberculosis en bovinos lecheros de la zona de lima detectado mediante tres técnicas diagnósticas
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Bustamante V, José

; Aguilar O, José

; Ortiz M, Martín

; Bustamante L, José

Revista de Investigaciones Veterinarias del Perú

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dec 2011, Volume 22 N. 4 Pages 394 - 402

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (Map) in dairy cattle in Lima, Peru. The Intradermal Tuberculin Test was applied to 60 cows from three herds and serum samples were tested using two commercial ELISA kits for antibodies against Map. Twenty-two cows (36.7%) were positive to at least to one of these diagnostic techniques (8 to one of the ELISA tests and 18 to the intradermal test). Feacal samples from the 22 positive animals were cultured on Herrold’s egg-yolk medium (HEYM) yielding five Map positive samples. PCR was used in the isolated strains and Map DNA was confirmed in two cows. A slight concordance (0.15, Kappa test) was found between techniques evaluating cellular and humoral immune response, establishing that these tests are not interchangeable (5.55> 3.84, Mc Nemar test). The results confirm the presence of Map in dairy cattle in Lima, Peru.

El presente estudio tuvo como objetivo evidenciar la presencia de Mycobacterium avium subsp. paratuberculosis (Map), en el ganado bovino lechero del departamento de Lima, Perú. Se evaluaron 60 vacas procedentes de tres establos, mediante la prueba tuberculínica intradérmica y dos kits comerciales de ELISA específicos contra Mycobacterium avium subsp. paratuberculosis. Resultaron positivos 22 animales (36.7%) a una de las tres técnicas diagnósticas (8 a una de las pruebas de ELISA y 18 a la prueba intradérmica). Se tomó muestras de heces a los 22 animales positivos y se cultivaron en el medio de Herrold con yema de huevo, resultando cinco muestras positivas al aislamiento de Map. Asimismo, se realizó el PCR a partir del cultivo, encontrándose dos muestras positivas. Se encontró una concordancia ligera (0.15, prueba Kappa) entre las técnicas que evalúan la respuesta inmune celular y humoral y se estableció que estas pruebas no son reemplazables (5.55>3.84, prueba de Mc Nemar). Los resultados permitieron evidenciar la presencia de Map en ganado bovino lechero de Lima, Perú.

13.

Estudio de la función lagrimal y la superficie conjuntival en pacientes diabéticos
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Figueroa-Ortiz, L.C.

; Jiménez Rodríguez, E.

; García-Ben, A.

; García-Campos, J.

Archivos de la Sociedad Española de Oftalmología

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apr 2011, Volume 86 N. 4 Pages 107 - 112

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Objectives: This study was performed to investigate whether different grades of diabetic retinopathy correlate with tear and ocular surface function in diabetic patients without subjective symptoms of dry eye. Methods: A total of 129 eyes of 72 patients without dry eye, assessed using McMonnies questionnaire were recruited to the study. The tear film and ocular surface were evaluated using the Schirmer test, tear film break-up time (BUT), fluorescein and lissamine green staining test, and conjunctival impression cytology. The results were analysed. Results: A normal Schirmer test and BUT was obtained in 51 and 67% of the eyes of diabetics patients, respectively. Fluorescein and lissamine green staining were negative in 81 and 95% of the eyes. The area and density of goblet cells was 580.46±370µm² and 235.58±109.03 goblets cell/mm², respectively. Conclusions: Diabetic retinopathy patients without subjective symptoms of dry eye and normal Schirmer and BUT test showed pathological grades of squamous metaplasia.

Objetivos: Analizar la relación entre la función lagrimal y la superficie conjuntival con los diferentes grados de retinopatía en pacientes diabéticos sin sintomatología subjetiva de ojo seco. Métodos: Se estudiaron 129 ojos de 72 pacientes sin sintomatología subjetiva de ojo seco según el cuestionario de Mc Monnies. La secreción lagrimal y la superficie ocular fueron evaluadas mediante la prueba de Schirmer, la prueba de tiempo de rotura de la película lagrimal (BUT), los grados de tinción con fluoresceína y verde lisamina, y la citología conjuntival de impresión. Se analizaron los resultados obtenidos. Resultados: El 51 y el 67% de los ojos de pacientes diabéticos mostraron un test de Schirmer y BUT dentro de la normalidad. La tinción con fluoresceína y verde lisamina fue negativa respectivamente en el 81 y el 95% de los ojos. Los valores de área y densidad media de células caliciformes fueron 580,46±370µm² y 235,58±109,03 cél. caliciformes/mm². Conclusión: Los pacientes con retinopatía diabética sin sintomatología subjetiva de ojo seco, aun cursando con la prueba de Schirmer y BUT normal, pueden presentar grados patológicos de metaplasia escamosa.

1830 downloads Cited 3 times in SciELO

14.

Evaluación del valor nutricional de recetas típicas elaboradas con maíz común y biofortificado en el departamento del cauca, Colombia
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Imbachí-Narváez, Paola Catalina

; Gutiérrez, Dayron

; Ortiz, Darwin

; Pachón, Helena

Salud Pública de México

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aug 2010, Volume 52 N. 4 Pages 305 - 314

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Objective. Ten typical recipes from southwestern Colombia were evaluated at CIAT in Palmira, Colombia between 2008 and 2009. Material and Methods. The recipes were prepared in a traditional manner, using quality protein maize (QPM, with more tryptophan and lysine) or common maize (CM); each of these was either fresh kernel or dry grain, or white or yellow maize, per the recipe. The two types of maize were compared for their protein quality. Results. Tryptophan concentrations were 0.011-0.072% and 0.012-0.107% for CM and QPM, respectively. Tryptophan values for QPM recipes were greater than or equal to those for CM recipes. Soluble protein ranged from 2.359-14.783 g/kg for CM and 3.339-16.839 g/kg for QPM. Except for one recipe, soluble protein in QPM recipes was greater or equal to that of CM recipes. In vitro protein digestibility was 75-84% for recipes prepared with both maize types. Only four recipes had a statistically significant difference between QPM and CM with regard to digestibility. Conclusions. Most recipes prepared with QPM had a higher protein quality than those prepared with CM.

Objetivo. Investigar si el maíz QPM (con más triptófano y lisina) presentaba mejor calidad proteica que el maíz común (MC) utilizándolo en preparaciones colombianas. Material y métodos. Entre 2008 y 2009, en el CIAT, Palmira, Colombia, se evaluó la concentración de triptófano, proteína soluble (PS) y digestibilidad in vitro de proteína (DP) en 10 recetas, preparadas con QPM o MC. Resultados. El triptófano osciló entre 0.011 y 0.072% para MC y 0.012 y 0.107% para QPM. Las recetas de QPM presentaron mayor o igual concentración de triptófano que las recetas de MC. PS osciló entre 2.359 y 14.783 g/kg para MC, y 3.339 y 16.839 g/kg para QPM. Las recetas de QPM, exceptuando una, presentaron mayor o igual concentración de PS. DP estuvo entre 75 y 84% para ambos maíces. Sólo cuatro recetas presentaron diferencia de DP entre QPM y MC. Conclusiones. Las recetas de maíz QPM son de mejor calidad proteica.

6948 downloads Cited 1 time in SciELO

15.

Evaluación del valor nutricional de recetas típicas elaboradas con maíz común y biofortificado en el departamento del cauca, Colombia
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Imbachí-Narváez, Paola Catalina

; Gutiérrez, Dayron

; Ortiz, Darwin

; Pachón, Helena

Salud Pública de México

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aug 2010, Volume 52 N. 4 Pages 305 - 314

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

3567 downloads Cited 1 time in SciELO

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