In this work, the innovation process to take chemical technologies born in universities to the market is described. The discussion is structured on the stages: research/science, patenting, opportunity evaluation, scale-up and proof of concept that lead to two possible outputs: technology transfer or startup creation. The work also describes the activities of Escalab, a UFMG technology lab, in the innovation process: technological mapping/R&D for the industry, technology scale up, technology acceleration, open innovation programs with industry and the program “go-to-market” for the insertion of chemical startups in market. It is expected that this experience should motivate academic chemistry researchers to push their technologies to the market. described stages researchscience research science research/science patenting evaluation scaleup up outputs creation Escalab lab mappingRD mapping R D acceleration gotomarket go “go-to-market

Resumo As Câmaras Setoriais e Temáticas são fóruns de diálogo criados pelo Ministério da Agricultura e Pecuária (MAPA), por meio dos quais se busca uma maior interação com as cadeias produtivas, bem como a identificação de oportunidades e a definição de ações prioritárias para o desenvolvimento da agricultura brasileira. As Câmaras Temáticas podem contribuir para o desenvolvimento da agricultura identificando demandas do setor produtivo e vinculando-as a propostas de pesquisa, desenvolvimento e inovação, criando agendas de inovação. Para isso, a análise das cadeias produtivas e suas práticas permite uma melhor compreensão do agronegócio, o que, consequentemente, possibilita o desenvolvimento de estratégias eficazes para promover o crescimento e desenvolvimento do setor. O objetivo de uma agenda em Flores, Gramas e Plantas Ornamentais é apresentar uma análise dos principais problemas e oportunidades de inovação nas cadeias produtivas. Um diagnóstico da situação atual, focado em uma visão para o futuro, foi necessário para a construção desta Agenda de Inovação. A abordagem metodológica foi dividida e conduzida por meio de diferentes análises: a) Análise da evolução do Mercado de Flores e Plantas Ornamentais; b) Análise das contribuições, impacto e expectativas dos elos da cadeia produtiva; c) Mapeamento do capital humano na ciência brasileira com potencial para contribuir com inovações tecnológicas para o mercado de flores, gramas e plantas ornamentais. A Agenda de Inovação prioriza os obstáculos mais relevantes, estabelece seu nível de importância e identifica dez Desafios de Inovação significativos a serem abordados pelos setores público e privado do país. Por meio da agenda, ações estratégicas são sugeridas para pesquisa, transferência de tecnologia e políticas públicas, essenciais para o desenvolvimento tecnológico do setor, sem negligenciar sua sustentabilidade socioeconômica e ambiental. Para cada Desafio de Inovação, foram sugeridas Ações Estratégicas a serem perseguidas, e o Brasil possui a competência técnica distribuída por todo o país, em universidades e institutos de pesquisa e empresas reconhecidas nacionalmente. MAPA, MAPA , (MAPA) vinculandoas vinculando isso agronegócio que consequentemente atual futuro análises b contribuições produtiva c flores ornamentais relevantes país públicas ambiental perseguidas nacionalmente (MAPA

Abstract Sectoral and Thematic Chambers are forums for dialogue created by the Ministry of Agriculture and Livestock (MAPA), through which greater interaction with productive chains is sought, as well as the identification of opportunities and the definition of priority actions for developing Brazilian agriculture. The Thematic Chambers can contribute to the development of agriculture by identifying demands from the productive sector and linking them to proposals for research, development, and innovation by creating innovation agendas. For that, the analysis of productive chains and their practices allows a better understanding of agribusiness, which consequently enables the development of effective strategies to promote the growth and development of a sector. The objective of the agenda in Flower, Turfgrass, and Ornamental Plant is to present an analysis of the main problems and opportunities for innovation in the productive chains. A diagnosis of the current situation focusing on a vision for the future was required to prepare this Innovation Agenda. The methodological approach was divided and conducted through different analyses: a) Analysis of the evolution of the Flower and Ornamental Plant Market; b) Analysis of contributions, impact, and expectations of the links in the production chain; c) Mapping of human capital in Brazilian science with the potential to contribute to technological innovations for the market of flowers, grasses, and ornamental plants. The Innovation Agenda prioritizes the most relevant obstacles, establishes their level of importance, and identifies ten significant Innovation Challenges to be addressed by the public and private sectors of the country. Through the agenda, strategic actions are suggested for research, technology transfer, and public policies, which are essential for the technological development of the sector, without neglecting its socioeconomic and environmental sustainability. For each Innovation Challenge, Strategic Actions were suggested to be pursued, and Brazil has the technical competence distributed across the country, in nationally recognized universities and research institutes and enterprises. MAPA, MAPA , (MAPA) sought agendas that agribusiness Turfgrass analyses Market b contributions impact chain c flowers grasses plants obstacles importance country transfer policies sustainability Challenge pursued enterprises (MAPA

Abstract Introduction Almost 20 % of patients with Non-Seminomatous Germinative Cell Tumors (NSGCT) will require intrathoracic metastasectomy after chemotherapy. The authors aim to determine their long-term survival rates. Methods Retrospective study including patients with NSGCT and intrathoracic metastasis after systemic therapy from January 2011 to June 2022. Treatment outcomes and overall survival were analyzed with the Kaplan-Meier method. Results Thirty-seven male patients were included with a median age of 31.8 years. Six presented with synchronous mediastinum and lung metastasis, nine had only lung, and 22 had mediastinal metastasis. Over half had retroperitoneal lymph node metastasis. Twenty-two had dissimilar pathologies, with a discordance rate of 62 %. Teratoma and embryonal carcinoma were the prevalent primary tumor types, 40.5 % each, while teratoma was predominant (70.3 %) in the metastasis group. Thoracotomy was the main surgical approach (39.2 %) followed by VATS (37.2 %), cervico-sternotomy (9.8 %), sternotomy (5.8 %), and clamshell (3.9 %). Lung resection was performed in 40.5 % of cases. Overall, 10-year survival rates were 94.3 % with no surgical-related mortality. Conclusion Multimodality treatment with systemic therapy followed by radical surgery offers a high cure rate to patients with intrathoracic metastatic testicular germ cell tumors. 2 NonSeminomatous Non Seminomatous (NSGCT chemotherapy longterm long term 201 2022 KaplanMeier Kaplan Meier method Thirtyseven Thirty seven 318 31 8 31. years Twentytwo Twenty two pathologies 6 types 405 40 5 40. each 70.3 703 70 3 (70. group 39.2 392 39 (39. 37.2 372 37 (37. %, , cervicosternotomy cervico 9.8 98 9 (9. 5.8 58 (5. 3.9 (3. . cases Overall 10year year 10 943 94 94. surgicalrelated related mortality tumors 202 4 70. 7 (70 39. (39 37. (37 9. (9 5. (5 3. (3 1 (7 (

RESUMO Objetivo: Caracterizar os fatores ambientais de crianças e adolescentes com paralisia cerebral (PC) no estado de Minas Gerais (MG), Brasil. Métodos: Trata-se de um estudo transversal envolvendo 164 cuidadores de crianças/adolescentes com PC, na faixa etária de um a 14 anos. O Sistema de Classificação da Função Motora Grossa (GMFCS) e o Sistema de Classificação da Habilidade Manual (MACS) foram utilizados para classificar a funcionalidade dos participantes e os fatores ambientais foram avaliados por um questionário on-line que abordou produtos e tecnologias, ambiente físico, serviços e sistemas. Análises descritivas foram realizadas por meio de porcentagem e frequência. Resultados: A maioria dos participantes tinha PC bilateral (66,9%) e 45% deles eram espásticos. Os níveis II e V do GMFCS e MACS foram os mais frequentes. Cerca de metade (49,4%) fazia uso de anticonvulsivantes, 27,4% realizaram aplicação de toxina botulínica e 29% cirurgia ortopédica em membros inferiores. Utilizavam órteses em membros inferiores 71,3% e eram usuários do sistema público de saúde 51,8%. A maioria tinha acesso à fisioterapia (91,5%), mas dificuldade de acesso a intervenções com outros profissionais, como psicólogos (28%) e nutricionistas (37,8%). A escola foi o ambiente mais frequentado (78%) e também mais adaptado estruturalmente (42,7%). Conclusões: Os resultados deste estudo sugerem que barreiras de acesso aos serviços de saúde e barreiras no ambiente físico podem impactar a participação e inclusão social. Objetivo (PC MG, MG , (MG) Brasil Métodos Tratase Trata se 16 criançasadolescentes 1 anos (GMFCS (MACS online on line tecnologias sistemas frequência Resultados 66,9% 669 66 9 (66,9% 45 espásticos frequentes 49,4% 494 49 4 (49,4% anticonvulsivantes 274 27 27,4 29 713 71 3 71,3 518 51 8 51,8% 91,5%, 915 91,5% 91 5 (91,5%) profissionais 28% 28 (28% 37,8%. 378 37,8% . 37 (37,8%) 78% 78 (78% 42,7%. 427 42,7% 42 7 (42,7%) Conclusões social (MG 66,9 6 (66,9 49,4 (49,4 2 27, 71, 51,8 91,5 (91,5% (28 37,8 (37,8% (78 42,7 (42,7% 66, (66, 49, (49, 51, 91, (91,5 (2 37, (37,8 (7 42, (42,7 (66 (49 (91, ( (37, (42, (6 (4 (91 (37 (42 (9 (3

ABSTRACT Objective: To characterize the environmental factors of children and adolescents with Cerebral Palsy (CP) in the state of Minas Gerais (MG), Brazil. Methods: This is a cross-sectional study involving 164 caregivers of children/adolescents with CP, aged 1-14 years. The Gross Motor Function Classification System (GMFCS) and the Manual Ability Classification System (MACS) were used to classify the participants’ functioning, and environmental factors were evaluated by an on-line questionnaire that examined products and technologies, physical environment, services, and systems. A descriptive analysis was performed using percentage and frequency. Results: Most participants had bilateral CP (66.9%) and 45% of them were spastic. Levels II and V of the GMFCS and MACS were the most frequent. About half (49.4%) used anticonvulsants, 27.4% underwent botulinum toxin application, and 29% went through orthopedic surgery in the lower limbs. Among the participants, 71.3% used orthoses in the lower limbs, and 51.8% used the public health care system. Most had access to physiotherapy (91.5%), but found difficulties to access interventions with other professionals, such as psychologists (28%) and nutritionists (37.8%). The school was the most frequently adapted environment (78%), and had the highest level of structural adaptation (42.7%). Conclusions: The results of this study suggest that the barriers to access health services and barriers to the physical environment may impact participation and social inclusion. Objective (CP MG, MG , (MG) Brazil Methods crosssectional cross sectional 16 childrenadolescents 114 1 14 1-1 years (GMFCS (MACS functioning online on line technologies systems frequency Results 66.9% 669 66 9 (66.9% 45 spastic frequent 49.4% 494 49 4 (49.4% anticonvulsants 274 27 27.4 application 29 limbs 713 71 3 71.3 518 51 8 51.8 system 91.5%, 915 91.5% 91 5 (91.5%) professionals 28% 28 (28% 37.8%. 378 37.8% . 37 (37.8%) 78%, 78 78% (78%) 42.7%. 427 42.7% 42 7 (42.7%) Conclusions inclusion (MG 11 1- 66.9 6 (66.9 49.4 (49.4 2 27. 71. 51. 91.5 (91.5% (28 37.8 (37.8% (78% 42.7 (42.7% 66. (66. 49. (49. 91. (91.5 (2 37. (37.8 (78 42. (42.7 (66 (49 (91. ( (37. (7 (42. (6 (4 (91 (37 (42 (9 (3

Este estudo teve como objetivo investigar a ocorrência de afecções respiratórias em crianças expostas à poeira de resíduos de mineração após o desastre do rompimento da barragem em Brumadinho, Minas Gerais, Brasil. A população de estudo incluiu crianças com idades entre 0 e 6 anos, residentes em três comunidades expostas à resíduos de poeira de mineração (Córrego do Feijão, Parque da Cachoeira e Tejuco) e uma comunidade não exposta (Aranha). A coleta de dados ocorreu entre 19 e 30 de julho de 2021, por meio de questionários que abordavam informações sociodemográficas e um inquérito recordatório sobre sinais, sintomas e doenças respiratórias. Foram avaliadas 217 crianças, sendo 119 das comunidades expostas e 98 da comunidade não exposta. Os residentes nas comunidades expostas relataram aumento na frequência de faxina em suas residências (p = 0,04) e no tráfego de veículos (p = 0,03). Entre as crianças de 4 anos, foi observada uma maior frequência de afecções das vias aéreas superiores (p = 0,01) e inferiores (p = 0,01), bem como de alergia respiratória (p = 0,05). O grupo exposto apresentou 1,5 vez mais relatos de alergia respiratória (75%; p = 0,02) em comparação com o não exposto (50,5%). Crianças que viviam nas comunidades expostas à poeira de resíduos apresentaram três vezes mais chance (OR ajustada = 3.63; IC95%: 1,37; 9,57) de ocorrência de alergia respiratória em comparação com as não expostas. Dois anos e seis meses após a ocorrência do desastre ambiental, as crianças das comunidades afetadas pelos resíduos das atividades de mineração e remediação permaneciam expostas à poeira com efeitos tóxicos sobre a saúde respiratória. Brumadinho Gerais Brasil Córrego Feijão Tejuco Aranha. Aranha . (Aranha) 1 3 2021 sinais 21 11 9 0,04 004 04 0,03. 003 0,03 03 0,03) 0,01 001 01 0,01, , 0,05. 005 0,05 05 0,05) 15 5 1, 75% 75 (75% 0,02 002 02 50,5%. 505 50,5% 50 (50,5%) OR 3.63 363 63 IC95% IC95 IC 1,37 137 37 9,57 957 57 ambiental (Aranha 202 2 0,0 00 7 (75 50,5 (50,5% 3.6 36 IC9 1,3 13 9,5 95 20 0, (7 50, (50,5 3. 9, ( (50, (50 (5

This study aimed to investigate the occurrence of respiratory diseases in children exposed to dust from mining waste after the Brumadinho dam disaster, Minas Gerais State, Brazil. The study population included children aged 0-6 years, living in three communities exposed to mining waste dust (Córrego do Feijão, Parque da Cachoeira, and Tejuco) and one unexposed community (Aranha). Data were collected from July 19 to 30, 2021, using questionnaires that addressed sociodemographic information and a recall survey on signs, symptoms, and respiratory diseases. A total of 217 children were evaluated, 119 living in the exposed communities and 98 in the non-exposed community. The residents in the exposed communities reported an increase in the frequency of home cleaning (p = 0.04) and in vehicular traffic (p = 0.03). Among children aged four, a higher frequency of upper (p = 0.01) and lower (p = 0.01) airway disorders, as well as respiratory allergy (p = 0.05) was observed. The exposed group had 1.5 times more reports of respiratory allergy (75%; p = 0.02) compared to the non-exposed group (50.5%). Children living in communities exposed to waste dust were three times more likely (adjusted OR = 3.63; 95%CI: 1.37; 9.57) to have respiratory allergies than those not exposed. Two years and six months after the environmental disaster occurred, children living in the communities affected by waste from mining and remediation activities remained exposed to dust with harmful effects on respiratory health. State Brazil 06 0 6 0- Córrego Feijão Cachoeira Tejuco Aranha. Aranha . (Aranha) 1 30 2021 signs symptoms 21 evaluated 11 9 nonexposed non 0.04 004 04 0.03. 003 0.03 03 0.03) four 0.01 001 01 disorders 0.05 005 05 observed 15 5 1. 75% 75 (75% 0.02 002 02 50.5%. 505 50.5% 50 (50.5%) adjusted 3.63 363 3 63 95%CI 95CI CI 95 1.37 137 37 9.57 957 57 occurred health (Aranha 202 2 0.0 00 7 (75 50.5 (50.5% 3.6 36 1.3 13 9.5 20 0. (7 50. (50.5 3. 9. ( (50. (50 (5

El objetivo de este estudio fue investigar la ocurrencia de enfermedades respiratorias en niños expuestos al polvo de residuos de la minería tras el desastre del colapso de la represa en Brumadinho, Minas Gerais, Brasil. La población de estudio incluyó niños que tenían entre 0 y 6 años, que viven en tres comunidades expuestas a residuos de polvo de la minería (Córrego do Feijão, Parque da Cachoeira y Tejuco) y una comunidad no expuesta (Aranha). Se recolectaron los datos entre el 19 y el 30 de julio de 2021, a través de cuestionarios que abordaban informaciones sociodemográficas y una encuesta recordatoria acerca de los señales, síntomas y enfermedades respiratorias. Se evaluaron 217 niños, de los cuales 119 viven en las comunidades expuestas y 98 viven en la comunidad no expuesta. Los residentes de las comunidades expuestas relataron un aumento en la frecuencia de limpieza de sus casas (p = 0,04) y en el tráfico de vehículos (p = 0,03). Entre los niños de 4 años, se observó una frecuencia más alta de enfermedades de las vías aéreas superiores (p = 0,01) e inferiores (p = 0,01), así como de alergia respiratoria (p = 0,05). El grupo expuesto presentó 1,5 veces más relatos de alergia respiratoria (el 75%; p = 0,02) en comparación con el grupo no expuesto (el 50,5%). Niños que vivían en las comunidades expuestas al polvo de residuos presentaron tres veces más probabilidad (OR ajustada = 3,63; IC95%: 1,37; 9,57) de ocurrencia de alergia respiratoria en comparación con los niños que no se expusieron. Dos años y seis meses tras el desastre ambiental, los niños que viven en las comunidades afectadas por los residuos de las actividades de minería y descontaminación permanecían expuestos al polvo con efectos tóxicos para la salud respiratoria. Brumadinho Gerais Brasil Córrego Feijão Tejuco Aranha. Aranha . (Aranha) 1 3 2021 señales 21 11 9 0,04 004 04 0,03. 003 0,03 03 0,03) 0,01 001 01 0,01, , 0,05. 005 0,05 05 0,05) 15 5 1, 75% 75 0,02 002 02 50,5%. 505 50,5% 50 50,5%) OR 3,63 363 63 IC95% IC95 IC 1,37 137 37 9,57 957 57 expusieron ambiental (Aranha 202 2 0,0 00 7 50,5 3,6 36 IC9 1,3 13 9,5 95 20 0, 50, 3, 9,

BACKGROUND Leishmania tarentolae is a non-pathogenic species found in lizards representing an important model for Leishmania biology. However, several aspects of this Sauroleishmania remain unknown to explain its low level of virulence. OBJECTIVES We reported several aspects of L. tarentolae biology including glycoconjugates, proteolytic activities and metabolome composition in comparison to pathogenic species (Leishmania amazonensis, Leishmania braziliensis, Leishmania infantum and Leishmania major). METHODS Parasites were cultured for extraction and purification of lipophosphoglycan (LPG), immunofluorescence probing with anti-gp63 and resistance against complement. Parasite extracts were also tested for proteases activity and metabolome composition. FINDINGS Leishmania tarentolae does not express LPG on its surface. It expresses gp63 at lower levels compared to pathogenic species and, is highly sensitive to complement-mediated lysis. This species also lacks intracellular/extracellular activities of proteolytic enzymes. It has metabolic differences with pathogenic species, exhibiting a lower abundance of metabolites including ABC transporters, biosynthesis of unsaturated fatty acids and steroids, TCA cycle, glycine/serine/threonine metabolism, glyoxylate/dicarboxylate metabolism and pentose-phosphate pathways. MAIN CONCLUSIONS The non-pathogenic phenotype of L. tarentolae is associated with alterations in several biochemical and molecular features. This reinforces the need of comparative studies between pathogenic and non-pathogenic species to elucidate the molecular mechanisms of virulence during host-parasite interactions. nonpathogenic non However L glycoconjugates amazonensis braziliensis major. major . major) LPG, , (LPG) antigp63 antigp anti gp anti-gp6 complement surface gp6 complementmediated mediated lysis intracellularextracellular intracellular extracellular enzymes transporters steroids cycle glycineserinethreonine glycine serine threonine glyoxylatedicarboxylate glyoxylate dicarboxylate pentosephosphate pentose phosphate pathways features hostparasite host parasite interactions (LPG antigp6 anti-gp

ABSTRACT Objectives: To estimate the prevalence of allergic rhinitis (AR), atopic dermatitis (AD), and wheezing, and to describe their patterns of co-occurrence according to different characteristics in adolescence and early adulthood. Methods: Cross-sectional analyses from the 15-year and 22-year follow-ups of the 1993 Pelotas (Brazil) Birth Cohort. The outcomes were assessed based on self-reported data, and the patterns of co-occurrence were determined using cluster analysis. The sample was described using absolute and relative frequencies according to the independent variables. Venn diagrams were generated to visualize the co-occurrence of AR, AD, and wheezing. Results: Data on AR, AD, and wheezing were available for 4,286 participants at 15 years and 3,789 at 22 years. At 15 years, AR was reported by 20.9% of participants, AD by 25.2%, and wheezing by 33.4%. Meanwhile, at 22 years, AR was reported by 24.6%, AD by 14.2%, and wheezing by 30.7%. Notably, the overlap between AR and wheezing was greater than that of the other conditions (6.9% at 15 years and 8.3% at 22 years). Participants with lower maternal education and lower income were more likely to report having “no health condition”. At 15 years, White individuals most frequently reported “three conditions” (4.1%; p<0.001), whereas at 22 years, they primarily reported “two conditions” (15.6%; p<0.001). The co-occurrence of all three health conditions was found to be greater than expected, with an observed rate 2.1 times higher (95% CI 1.4 - 3.0) at 22 years. Conclusions: This study highlights the social gradient in the diagnosis and reporting of co-occurrence of AR, AD, and wheezing. Objectives , (AR) (AD) cooccurrence co occurrence adulthood Methods Crosssectional Cross sectional 15year year 22year followups follow ups 199 Brazil (Brazil Cohort selfreported self data analysis variables Results 4286 4 286 4,28 1 3789 3 789 3,78 2 209 20 9 20.9 252 25 25.2% 334 33 33.4% Meanwhile 246 24 6 24.6% 142 14 14.2% 307 30 7 30.7% Notably 6.9% 69 (6.9 83 8 8.3 . years) no condition. condition condition” 4.1% 41 (4.1% p<0.001, p0001 p p<0.001 0 001 p<0.001) two 15.6% 156 (15.6% p<0.001. expected 21 2. 95% 95 (95 1. 3.0 Conclusions (AR (AD 19 428 28 4,2 378 78 3,7 20. 25.2 33.4 24.6 14.2 30.7 6.9 (6. 8. 4.1 (4.1 p000 p<0.00 00 15.6 (15.6 (9 3. 42 4, 37 3, 25. 33. 24. 14. 30. 6. (6 4. (4. p00 p<0.0 15. (15. ( (4 p0 p<0. (15 p<0 (1 p<

Abstract This study aims to evaluate the potential offered by the Red Riding Hood Unplugged game as a didactic resource for the development of computational thinking in students with visual impairments. The methodology consists of a qualitative approach, with exploratory/descriptive objectives. The subjects are three students with congenital visual impairment. The research instrument used was participant observation, through a field diary. To analyze the data, categorical analysis was used, based on four categories, decomposition, pattern recognition, abstraction and algorithm. From the discussion of the results found, it is possible to conclude that the Unplugged Little Red Riding Hood board game has potential for the development of concepts of computational thinking, with an activity that promotes the Unplugged Programming Language, through the use of accessible didactic resources, favoring cognitive development, logical/mathematical reasoning in problem solving and inclusion.

Resumo Este estudo tem por objetivo avaliar o potencial oferecido pelo jogo Chapeuzinho Vermelho Desplugada como recurso didático para o desenvolvimento do pensamento computacional em alunos com deficiência visual. A metodologia consiste em uma abordagem qualitativa, com objetivos exploratório/descritivos. Os sujeitos são três estudantes com deficiência visual congênita. O instrumento de pesquisa utilizado foi a observação participante, por meio do diário de campo. Para analisar os dados, empregou-se a análise categorial, a partir de quatro categorias: decomposição, reconhecimento de padrões, abstração e algoritmo. A partir da discussão dos resultados encontrados, é possível concluir que o jogo de tabuleiro Chapeuzinho Vermelho Desplugada apresenta potencial para o desenvolvimento de conceitos do pensamento computacional, com uma atividade que promova a Linguagem de Programação Desplugada, por meio do uso de recursos didáticos acessíveis, favorecendo o desenvolvimento cognitivo, o raciocínio lógico/matemático na resolução de problemas e a inclusão.

ABSTRACT Acute wound dressings can be based on PVA hydrogels, which present many characteristics of an ideal dressing, e.g., fluid uptake, a moisturized environment, etc. The lack of antimicrobial properties leads to the addition of natural active agents. The present work aims to manufacture and compare PVA gels loaded with Barbatimão bark extract, Leucaena bark extract, Aloe vera, and Lavender essential oil. They were characterized by FTIR, swelling tests, SEM, actives release. There were interactions between PVA and the active agents. The addition of Leucaena and Barbatimão increased the PVA ability to swell, but the opposite was found for Aloe vera and Lavender essential oil. PVA-Lavender essential oil samples presented interconnected pores, while samples with essential oil or extracts presented high crystallinity. Lavender essential oil and Aloe vera presented the highest release. The Barbatimão and Leucaena samples’ release may be related to the samples’ swelling, but the initial release of Aloe vera and Lavender samples was diffusion controlled by swelling. Their long-term release was dose-dependent for Aloe vera, while it was a non-Fickian diffusion for Lavender essential oil related to the hydrogel’s relaxation step. There is a synergistic effect when Aloe vera and Lavender essential oil are loaded in PVA hydrogels.

RESUMO: Objetivo: Apresentar o protocolo da pesquisa e descrever os resultados preliminares da linha de base da população de estudo do Projeto Bruminha. Métodos: Este projeto é parte do conjunto de ações propostas no âmbito do Programa de Ações Saúde Brumadinho. Trata-se de um estudo de coorte prospectiva, com seguimento periódico por quatro anos, cuja população elegível foi constituída de todas as crianças de 0 a 6 anos de idade residentes em quatro localidades situadas na zona rural do município afetado, com coleta de dados sociodemográficos e de saúde e de amostras urinárias para avaliação da exposição a metais. Resultados: No primeiro ano de estudo foi avaliada 62% (217) da população elegível e se coletaram 172 (79%) amostras de urina válidas. Em todas as amostras analisadas foi detectado pelo menos um metal, e em 50,6% (n=87) concentrações urinárias acima do valor de referência. Em 38% (n=82) das crianças a avaliação do desenvolvimento neuropsicomotor foi considerada de risco. O relato de alergia respiratória foi quatro vezes (4,27) mais frequente e de bronquite 61% maior (1,62) nas crianças residentes nas localidades expostas à poeira de resíduos de minério, proporcionalmente àquelas residentes a mais de 10 km do local do desastre. Conclusão: O protocolo do estudo mostrou-se adequado para avaliação dos desfechos propostos. A estratégia de captação da população de estudo necessitou de reajustes quanto ao processo de sensibilização da comunidade a longo prazo com entrada de novos participantes nos próximos seguimentos (2022 e 2023). RESUMO Objetivo Bruminha Métodos Brumadinho Tratase Trata prospectiva afetado metais Resultados 62 217 (217 17 79% 79 (79% válidas metal 506 50 50,6 n=87 n87 n 87 (n=87 referência 38 n=82 n82 82 (n=82 risco 4,27 427 4 27 (4,27 61 1,62 162 1 (1,62 minério desastre Conclusão mostrouse mostrou propostos 2022 (202 2023. 2023 . 2023) 21 (21 7 (79 5 50, n=8 n8 8 (n=8 3 4,2 42 2 (4,2 1,6 16 (1,6 202 (20 (2 (7 n= (n= 4, (4, 1, (1, 20 ( (n (4 (1

ABSTRACT: Objective: To present the research protocol and to describe the preliminary results of the Bruminha Project. Methods: This project is part of the set of health actions proposed under the Brumadinho Health Project. It is a prospective cohort study with periodic follow-ups for four years. The eligible population was all children aged 0 to 6 years living in four rural communities of the affected municipality. Sociodemographic and health data were collected, as well as urinary samples, for assessment of exposure to metals. Results: In the first year of study, we evaluated 62% (217) of the eligible population and collected 172 (79%) valid urine samples. At least one metal was detected in all samples, and in 50.6% (n=87) or them, the metal concentration was higher than the reference value. In 38% (n=82) of the children, neuropsychomotor development was considered at risk. Report of respiratory allergy was four times (4.27) more frequent and of bronchitis 62% higher (1.62) in children living in locations exposed to dust from ore residues, compared to those living more than 10 km away from the site of the dam disaster. Conclusion: The study protocol was proven adequate to evaluate the proposed outcomes. The strategy used for population selection required adjustments regarding the long-term community awareness process, with new participants in the upcoming follow-ups (2022 and 2023). ABSTRACT Objective Project Methods followups follow ups municipality samples metals Results 62 217 (217 17 79% 79 (79% 506 50 50.6 n=87 n87 n 87 (n=87 them value 38 n=82 n82 82 (n=82 risk 4.27 427 4 27 (4.27 1.62 162 1 (1.62 residues disaster Conclusion outcomes longterm long term process 2022 (202 2023. 2023 . 2023) 21 (21 7 (79 5 50. n=8 n8 8 (n=8 3 4.2 42 2 (4.2 1.6 16 (1.6 202 (20 (2 (7 n= (n= 4. (4. 1. (1. 20 ( (n (4 (1