Abstract Objective To evaluate the bone repair associated with the use of biphasic ceramics based on hydroxyapatite and β-tricalcium phosphate (HA/TCP) in the procedures of maxillary sinus membrane elevation in human beings. Material and method Ten patients with a residual bone ridge in the posterior maxillary region between 3 mm and 5 mm in height were selected and indicated for procedures of bone grafting associated with maxillary sinus lift procedure. The maxillary sinuses were filled with HA/TCP, and after 8 months, the implants were implanted and a bone biopsy was collected with the aid of a trephine drill, which was then submitted to histological analysis for the evaluation of the composition of the grafted area. Result The histological description analysis of the biopsies revealed the presence of vital and immature bone tissue, juxtaposed to the biomaterial. Histomorphometric analysis showed that the biopsy composition was in average 28.8%, 27.4%, and 43.6% of bone tissue, biomaterial, and soft tissue, respectively. Conclusion The areas grafted with HA/TCP presented the adequate amount of bone formation that allowed the implantation of the implants. The success of the grafting procedure with this biomaterial was associated with its osteoconduction potential that allowed the formation of bone tissue in close contact with HA/TCP.
Resumo Objetivo Avaliar o reparo ósseo associado ao uso da cerâmica bifásica a base de hidroxiapatita e β-tricálcio fosfato (HA/TCP) nos procedimentos de elevação da membrana do seio maxilar em humanos. Material e método Foram selecionados 10 pacientes com rebordo ósseo residual entre 3 mm e 5 mm de altura na região posterior da maxila e que tinham indicação para procedimentos de enxertia óssea associado ao levantamento de seio maxilar. Os seios maxilares foram preenchidos com HA/TCP, e após 8 meses, foi executado a instalação dos implantes e nesse momento foi realizada a coleta de um fragmento ósseo com auxílio de uma broca trefina, o qual foi submetido à análise histológica da área enxertada. Resultado A análise histológica das biópsias revelou a presença de tecido ósseo vital e imaturo, justaposto ao biomaterial. A análise histomorfométrica revelou percentuais de 28,8%, 27,4% e 43,6% para tecido ósseo, biomaterial e tecido mole, respectivamente. Conclusão As áreas enxertadas com a HA/TCP apresentaram quantidade adequada de formação óssea que permitiu a instalação dos implantes. O sucesso do procedimento de enxertia com esse biomaterial esteve associado ao seu potencial de osteocondução que permitiu a formação de tecido ósseo em íntimo contato com a HA/TCP.
ABSTRACT The study evaluated the effects of brown flaxseed supplementation in natura on the prevention of DNA damage induced by 1,2-dimethylhydrazine (DMH) in vivo. The experimental groups were Negative and Positive Controls and the protocols of Pre-treatment, Simultaneous, Post-treatment, Pre+continuous in relation to the supplementation of brown flaxseed and administration with carcinogenic compound. The results showed that brown flaxseed supplementation does not cause genomic and genetic damage. In addition, brown flaxseed showed a chemopreventive food that reduced the damages assessed by the comet assay up to 94.07x and the damages assessed by the micronucleus assay up to 91.88x. Brown flaxseed supplementation also increased the frequency of monocytes and lymphocytes indicating immunological improvements. Thus, brown flaxseed supplementation is considered safe and reduces the frequency of DNA damage that can lead to tumors. Therefore, if these events are confirmed in humans, flaxseed will have reinforced its indication as a functional chemopreventive food in the prevention of cancer.