Abstract This work reports how the solar conversion efficiency of dye-sensitized solar cells (DSSCs) depends on the crystalline structure of both the compact TiO2 blocking layer (BL) and homoepitaxially grown porous TiO2 mesoporous structure. The films were grown by simultaneous sputtering of titanium targets by DC magnetron and by high-power impulse magnetron systems (HiPIMS). The deposition conditions were managed to produce in situ dense BLs and porous TiO2 films. The only variable was the polarization of the BLs (0 to -200V). The polarization caused phase transformations from pure anatase phase through rutile-anatase mixed phases to rutile phase. The polarization results in decreasing intensity of the anatase (101) peak of the porous layers. The latter promptly decreased linearly the value of the short-current Isc and exponentially the open-circuit voltage Voc of the cells. Another inference is the surface energy of the BLs, which follows an exponential decay as a function of the film polarization. XPS study of the Ti 2p3/2 – Ti 2p1/2 doublet reveals an appearance of a shake-up satellite, whose area exponentially decreases as the polarization potential rises. This phenomenon is discussed and related with other physical aspects of the homo-epitaxially grown films with different texture. The anatase phase content and its purity predefined by the experimental conditions determine the quality of the DSSC, as well as other components such as the dye type, the electrolyte, and the electrode materials.

Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death was assessed by cell counting and measurement of lactate dehydrogenase activity. The limit of detection of the Vero cell assay was 10 ng/mL using a concentration of 1.6 x 10(5) cells/well. Solid-state fermentation (SSF) and treatment with calcium compounds were used as cake detoxification processes. For SSF, Aspergillus niger was grown using a castor cake as a substrate, and this cake was analyzed after 24, 48, 72, and 96 h of SSF. Ricin was eliminated after 24 h of SSF treatment. The cake was treated with 4 or 8% Ca(OH)2 or CaO, and both the toxicity and the allergenic properties were entirely abolished. A by-product free of toxicity and allergens was obtained.

Objetivou-se com este estudo avaliar os efeitos de fontes proteicas e da frequência de fornecimento de suplemento sobre o consumo e a digestibilidade dos nutrientes, as concentrações de nitrogênio amoniacal (N-NH3), o pH ruminal e o nitrogênio ureico no soro de bovinos recriados em pastagem de Brachiaria brizantha cv. Marandu no período seco. Foram utilizados cinco novilhos mestiços Nelore × Angus fistulados no rúmen, castrados, com peso médio inicial de 295 kg em um delineamento quadrado latino 5 × 5. Avaliaram-se dois suplementos isoproteicos, um com farelo de soja (FS) e outro com farelo de algodão de alta energia, fornecidos em duas frequências: diariamente (7 vezes por semana) e 3 vezes por semana (segundas, quartas e sextas-feiras); e mistura mineral (MM). A suplementação aumentou o consumo de PB, extrato etéreo (EE) e carboidratos não-fibrosos (CNF), em kg/dia. O suplemento com farelo de algodão o consumo de extrato etéreo. Houve efeito positivo da suplementação 3 vezes por semana sobre os consumos de matéria seca total, de forragem, de matéria orgânica de forragem, carboidratos totais (CT) e FDN. A suplementação reduziu a digestibilidade da FDN e aumentou a de PB. O suplemento com farelo de algodão reduziu a digestibilidade de FDN e carboidratos totais. A suplementação 3 vezes por semana não afetou a digestibilidade dos nutrientes nem o pH ruminal. O consumo de suplemento aumenta as concentrações de N-NH3, principalmente se realizado 3 vezes por semana. As concentrações de nitrogênio ureico no soro de bovinos em pastejo aumentam com o consumo de suplementos.

The objective of this study was to evaluate the effects of protein sources and frequency of supplement offer on the intake and nutrient digestibility, on the concentrations of ammonia nitrogen (N-NH3), on rumen pH and on serum urea nitrogen concentrations in steers grazing pasture of Brachiaria brizantha cv. Marandu during the dry season. Five crossbred Zebu × Angus steers with initial live weight of 295 kg, castrated and fitted with rumen canullae were used in a 5 × 5 Latin square design. It was evaluated two isoprotein supplements, one with soybean meal (SM) and the other with high energy cottonseed meal, offered at two frequencies: every day (7 times a week), or 3 times a week (on Mondays, Wednesdays and Fridays); and a mineral mixture (MM). The supplementation increased the intake of CP, of ether extract (EE) and non-fibrous carbohydrates (NFC) in kg/day. The supplement with cottonseed meal increased the intake of ether extract. There was a positive effect of the three times a week supplementation on the intakes of total dry matter, of forage, of organic matter of forage, total carbohydrates (TC) and NDF. The supplementation reduced the digestibility of the NDF and increased the digestibility of the CP. The supplement with cottonseed meal reduced digestibility of NDF and total carbohydrates. Three times a week supplementation did not affect digestibility of the nutrients neither the rumen pH. Supplement intake increases concentrations of N-NH3, especially if it is carried out 3 times a week. Concentrations of urea nitrogen in the serum of growing steers increase.

The association of plasma interleukin-6 (IL-6) levels, muscle strength and functional capacity was investigated in a cross-sectional study of community-dwelling elderly women from Belo Horizonte, Brazil. Elderly people who present controlled chronic diseases with no negative impact on physical, psychosocial and mental functionality are considered to be community-dwelling. Psychological and social stress due to unsuccessfully aging can represent a risk for immune system disfunctions. IL-6 levels, isokinetic muscle strength of knee flexion/extension, and functional tests to determine time required to rise from a chair and gait velocity were measured in 57 participants (71.21 ± 7.38 years). Serum levels of IL-6 were measured in duplicate and were performed within one single assay (mouse monoclonal antibody against IL-6; High-Sensitivity, Quantikine®, R & D Systems, USA; intra-assay coefficient of variance = 6.9-7.4%; interassay coefficient of variance = 9.6-6.5%; sensitivity = 0.016-0.110 pg/mL; mean = 0.039 pg/mL). Muscle strength was assessed with the isokinetic dynamometer Biodex System 3 Pro®. After the Shapiro-Wilk normality test was applied, correlations were investigated using Spearman and Kruskal-Wallis tests. Post hoc analysis was performed using the Dunn test. A significant negative correlation was observed between plasma IL-6 levels (1.95 ± 1.77 pg/mL) and muscle strength for knee flexion (70.70 ± 21.14%; r = -0.265; P = 0.047) and extension (271.84 ± 67.85%; r = -0.315; P = 0.017). No significant correlation was observed between IL-6 levels and the functional tests (time to rise from a chair = 14.65 ± 2.82 s and gait velocity = 0.95 ± 0.14 m/s). These results suggest that IL-6 is associated with reduced muscle strength.