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Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group.

https://doi.org/10.1590/0001-3765202320220502

Incubation variables, performance, and morphometry of the duodenal mucosa of Japanese quails (Coturnixcoturnix japonica) submitted to different incubation temperatures and thermally challenged after hatching
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Porto, M.L.

; Teófilo, T.S.

; Cavalcanti, D.M.L.P.

; Freitas, C.I.A.

; Oliveira, M.F.

; Fontenele-Neto, J.D.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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mar 2021, Volume 73 N. 2 Pages 495 - 507

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se avaliar os efeitos de diferentes temperaturas de incubação sobre as variáveis de incubação, desempenho e morfometria da mucosa duodenal de codornas japonesas (Coturnix coturnix japonica) submetidas ao estresse térmico crônico por calor após eclosão. Foram distribuídos 540 ovos em três incubadoras, com temperatura de 37,8°C e umidade 60%. A partir do sexto dia de incubação até a eclosão, as temperaturas foram ajustadas para 37,8°C, 38,5°C e 39,5°C. Após a eclosão, as codornas foram avaliadas quanto ao escore de qualidade, pesadas e distribuídas em um delineamento inteiramente ao acaso, com três temperaturas de incubação (37,8ºC, 38,5ºC e 39,5°C) e duas temperaturas ambientes (estresse e termoneutro). Aos 10, 20, 30 e 40 dias, foram pesadas para determinar o peso vivo (g) e o ganho de peso(g). Quatro codornas de cada tratamento foram eutanasiadas para coleta do duodeno, para determinar os parâmetros morfométricos. Os dados foram analisados e as diferenças entre as médias foram determinadas pelo teste de Tukey a 5%. A temperatura de incubação de 39,5°C proporcionou menor taxa de eclosão e menor peso vivo ao nascer, entretanto, a partir do 10° dia de idade, essa temperatura aumentou o peso vivo, o ganho de peso e influenciou positivamente os parâmetros morfológicos da mucosa duodenal em situações de estresse crônico.

ABSTRACT This study aimed to evaluate the effects of different temperatures on incubation variables, performance, and morphometry of the duodenal mucosa of Japanese quails (Coturnix coturnix japonica) submitted to chronic heat stress after hatching. We distributed 540 eggs in three incubators with a temperature of 37.8°C and 60% of humidity. From the 6th day of incubation until hatching, the temperatures were adjusted to (37.8°C, 38.5°C and 39.5°C). After hatching, quails were evaluated for the quality score, weighed, and distributed in a completely randomized design with three incubation temperatures (37.8, 38.5, and 39.5°C) and two ambient temperatures (stress and thermoneutral). At 10, 20, 30, and 40 days they were weighed to determine the live weight (g) and weight gain(g). To collect the duodenum and determine morphometric parameters, we euthanized four quails of each treatment. The data were analyzed, and the differences between the means determined by the Tukey test at 5%. The incubation temperature of 39.5°C provided lower hatching rate and the live weight at birth; however, from the 10th day of age, increased live weight, weight gain, and positively influenced the morphological parameters of the duodenal mucosa in situations of chronic stress.

https://doi.org/10.1590/1678-4162-12052 243 downloads

Priority conservation of medicinal woody plants from protected forests based on ecological and ethnobotanical data
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OLIVEIRA, SAMARA F.

; RANGEL, JULIANA M.L.

; MACEDO, JULIMERY G.F.

; SILVA, SAMILLE L.

; RIBEIRO, DAIANY A.

; MACEDO, MARIA SORAYA

; SEIXAS, EMÍDIA NAIANA C.

; MACÊDO, DELMACIA G.

; SARAIVA, MANUELE E.

; LINHARES, KARINA V.

; MENDONÇA, ANA CLEIDE A.M.

; SOUZA, MARTA MARIA A.

; ARAÚJO, ELCIDA L.

; SILVA, MARIA ARLENE P.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 elocation e20201769

Abstract: En | Text: En | PDF: En

Abstract The combination of ethnobotanical and ecological knowledge is an important tool in indicating priority species for conservation. We sought to gather ethnobotanical knowledge on the diversity and use of woody medicinal plants in the Chapada Araripe region, assessing the real availability of woody medicinal resources in the Araripe Forests in the cerrado and carrasco areas, and indicate priority species for conservation. A total of 107 species were recorded in the ethnobotanical surveys, classified into 39 families and 83 genera, of which 92 species, 36 families and 70 genera for the cerrado areas, and 47 species, 25 families and 39 genera, for the carrasco areas. 59% were present in the phytosociological surveys for cerrado and 38% for carrascos. Species with high versatility of medicinal use did not necessarily have high local availability, and some were not recorded in the sampling. Thirteen species in cerrados and four in carrascos were indicated as conservation priorities. Use not aligned of species with the reality of the present time can indeed affect the vegetation landscape, and in a future scenario, not taking local measures to conserve protected forest resources, besides increasing the lists of local conservation priorities, can affect economic practices, increasing social and environmental conflicts.

https://doi.org/10.1590/0001-3765202120201769 36 downloads

Tecnologías en la enseñanza en enfermería, innovación y uso de TICs: revisión integrativa
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Girão, A.L. Araújo

; Cavalcante, M.L. Silva Nunes

; Oliveira, I. Costa Lima de

; Aires, S. Freitas

; Oliveira, S.K. Paz de

; Carvalho, R.E. Fontenele Lima de

Enfermería universitaria

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dec 2020, Volume 17 N. 4 Pages 475 - 489

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo Introdução: A utilização de recursos digitais modernos para o ensino constitui uma estratégia para atender as novas necessidades de aprendizagem. No âmbito de enfermagem pode facilitar a inserção dos estudantes na prática clínica em modalidade virtual para que desenvolvam suas habilidades e competências. Objetivo: Analisar as evidências sobre a utilização de tecnologias no ensino de enfermagem. Métodos: Revisão integrativa realizada no portal de periódicos SciELO e nas bases de dados IBECS, LILACS, BDENF, Web of Science, PubMed e CINAHL. Resultados: Foi realizada a identificação inicial de 62 artigos, aplicaram-se os critérios de exclusão até o estabelecimento da amostra de 26 artigos. Após a leitura e a análi-se dos estudos, foi constatado que as tecnologias são mais utilizadas em conjunto com as aulas teóricas nas salas de aula, ou como plataformas de ensino online, ou também como ferramentas auxiliares para a prática de simulações realísticas. Discussão: O uso da internet foi predominante nos estudos, dado que favorece a atualização, a comunicação e a qualificação acadêmica. Sobre as plataformas digitais de ensino, o espa-ço virtual permite reunir e integrar diversos médios com finalidades diferentes possibilitando a criação de atividades variadas. Apresentaram-se diversos benefícios de imersão para o ensino através de simulações em um ambiente profissional desde a universidade. Conclusões: O estudo apresenta uma visão geral das tecnologias educacionais utilizadas no ensino de enfermagem e provê conhecimento de novas metodologias, o qual beneficia professores e estudantes.

Abstract Introduction: The utilization of modern digital resources in teaching constitutes a strategy to address the new learning needs. In the area of nursing, the use of digital resources can facilitate the insertion of students into the clinical practice by providing a virtual environment in which students can develop their skills and competencies. Objective: To analyze the evidence regarding the utilization of technologies in nursing teaching. Methods: This is an integrative review based on the SciELO publications and IBECS, LILACS, BDENF, Web of Science, PubMed and CINAHL databases. Results: 62 relevant articles were identified. After considering the exclusion criteria, a final sample with 26 articles was constituted. The studies were read and analyzed, and it was found that the diverse technologies are more frequently utilized in conjunction with theory classes in classrooms, as online teaching platforms, or as auxiliary tools to support realistic simulations. Discussion: The use of the Internet was prevalent in the analyzed studies. Regarding some teaching digital platforms, virtual spaces showed being useful to create diverse teaching-learning activities; with simulations being helpful to prepare students to enter the professional practice. Conclusions: This study presents a general view of the educational technologies utilized in nursing teaching, while displaying some specific methodologies which can be of benefit for both students and teachers.

Resumen Introducción: La utilización de los recursos digitales modernos para la enseñanza constituye una estrategia para atender las nuevas necesidades de aprendizaje. En el ámbito de enfermería puede facilitar la inserción de los estudiantes en la práctica clínica en modo virtual para que desarrollen sus habilidades y competencias. Objetivo: Analizar las evidencias sobre la utilización de tecnologías en la enseñanza de enfermería. Métodos: Revisión integrativa realizada en el portal de revistas SciELO y en las bases de datos IBECS, LILACS, BDENF, Web of Science, PubMed y CINAHL. Resultados: Se realizó la identificación inicial de 62 artículos, se aplicaron los criterios de exclusión hasta el establecimiento de la muestra de 26 artículos. Después de la lectura y análisis de los estudios, se constató que las tecnologías son más utilizadas junto con las clases teóricas en las aulas, o como plataformas online de enseñanza, o también como herramientas auxiliares para la práctica de simulaciones realísticas. Discusión: El uso de internet fue predominante en los estudios, pues favorece la actualización, la comunicación y la cualificación académica. En cuanto a las plataformas digitales de enseñanza, el espacio virtual permite reunir e integrar diversos medios con finalidades diferentes posibilitando la creación de actividades variadas. Para la enseñanza a través de simulaciones se presentaron diversos beneficios de inmersión a un ambiente profesional desde la universidad. Conclusiones: El estudio presenta una visión general de las tecnologías educativas utilizadas para la enseñanza de enfermería y provee conocimiento de nuevas metodologías, lo que beneficia a profesores y estudiantes.

https://doi.org/10.22201/eneo.23958421e.2020.4.763 620 downloads

Characteristics and outcomes of patients with COVID-19 admitted to the ICU in a university hospital in São Paulo, Brazil - study protocol
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Ferreira, Juliana C.

; Ho, Yeh-Li

; Besen, Bruno A.M.P.

; Malbuisson, Luiz M.S.

; Taniguchi, Leandro U.

; Mendes, Pedro V.

; Costa, Eduardo L.V.

; Park, Marcelo

; Daltro-Oliveira, Renato

; Roepke, Roberta M.L.

; Silva Jr, João M.

; Carmona, Maria José C.

; Carvalho, Carlos Roberto Ribeiro

; Hirota, Adriana

; Kanasiro, Alberto Kendy

; Crescenzi, Alessandra

; Fernandes, Amanda Coelho

; Miethke-Morais, Anna

; Bellintani, Arthur Petrillo

; Canasiro, Artur Ribeiro

; Carneiro, Bárbara Vieira

; Zanbon, Beatriz Keiko

; Batista, Bernardo Pinheiro De Senna Nogueira

; Nicolao, Bianca Ruiz

; Besen, Bruno Adler Maccagnan Pinheiro

; Biselli, Bruno

; Macedo, Bruno Rocha De

; Toledo, Caio Machado Gomes De

; Pompilio, Carlos Eduardo

; Carvalho, Carlos Roberto Ribeiro De

; Mol, Caroline Gomes

; Stipanich, Cassio

; Bueno, Caue Gasparotto

; Garzillo, Cibele

; Tanaka, Clarice

; Forte, Daniel Neves

; Joelsons, Daniel

; Robira, Daniele

; Costa, Eduardo Leite Vieira

; Silva Júnior, Elson Mendes Da

; Regalio, Fabiane Aliotti

; Segura, Gabriela Cardoso

; Marcelino, Gustavo Brasil

; Louro, Giulia Sefrin

; Ho, Yeh-Li

; Ferreira, Isabela Argollo

; Gois, Jeison de Oliveira

; Silva Junior, Joao Manoel Da

; Reusing Junior, Jose Otto

; Ribeiro, Julia Fray

; Ferreira, Juliana Carvalho

; Galleti, Karine Vusberg

; Silva, Katia Regina

; Isensee, Larissa Padrao

; Oliveira, Larissa dos Santos

; Taniguchi, Leandro Utino

; Letaif, Leila Suemi

; Lima, Lígia Trombetta

; Park, Lucas Yongsoo

; Chaves Netto, Lucas

; Nobrega, Luciana Cassimiro

; Haddad, Luciana

; Hajjar, Ludhmila

; Malbouisson, Luiz Marcelo

; Pandolfi, Manuela Cristina Adsuara

; Park, Marcelo

; Carmona, Maria José Carvalho

; Andrade, Maria Castilho Prandini H De

; Santos, Mariana Moreira

; Bateloche, Matheus Pereira

; Suiama, Mayra Akimi

; Oliveira, Mayron Faria de

; Sousa, Mayson Laercio

; Louvaes, Michelle

; Huemer, Natassja

; Mendes, Pedro

; Lins, Paulo Ricardo Gessolo

; Santos, Pedro Gaspar Dos

; Moreira, Pedro Ferreira Paiva

; Guazzelli, Renata Mello

; Reis, Renato Batista Dos

; Oliveira, Renato Daltro De

; Roepke, Roberta Muriel Longo

; Pedro, Rodolpho Augusto De Moura

; Kondo, Rodrigo

; Rached, Samia Zahi

; Fonseca, Sergio Roberto Silveira Da

; Borges, Thais Sousa

; Ferreira, Thalissa

; Cobello Junior, Vilson

; Sales, Vivian Vieira Tenório

; Ferreira, Willaby Serafim Cassa

Clinics

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2020, Volume 75 elocation e2294

Abstract: En | Text: En | PDF: En

OBJECTIVES: We designed a cohort study to describe characteristics and outcomes of patients with coronavirus disease (COVID-19) admitted to the intensive care unit (ICU) in the largest public hospital in Sao Paulo, Brazil, as Latin America becomes the epicenter of the pandemic. METHODS: This is the protocol for a study being conducted at an academic hospital in Brazil with 300 adult ICU beds dedicated to COVID-19 patients. We will include adult patients admitted to the ICU with suspected or confirmed COVID-19 during the study period. The main outcome is ICU survival at 28 days. Data will be collected prospectively and retrospectively by trained investigators from the hospital’s electronic medical records, using an electronic data capture tool. We will collect data on demographics, comorbidities, severity of disease, and laboratorial test results at admission. Information on the need for advanced life support and ventilator parameters will be collected during ICU stay. Patients will be followed up for 28 days in the ICU and 60 days in the hospital. We will plot Kaplan-Meier curves to estimate ICU and hospital survival and perform survival analysis using the Cox proportional hazards model to identify the main risk factors for mortality. ClinicalTrials.gov: NCT04378582. RESULTS: We expect to include a large sample of patients with COVID-19 admitted to the ICU and to be able to provide data on admission characteristics, use of advanced life support, ICU survival at 28 days, and hospital survival at 60 days. CONCLUSIONS: This study will provide epidemiological data about critically ill patients with COVID-19 in Brazil, which could inform health policy and resource allocation in low- and middle-income countries.

https://doi.org/10.6061/clinics/2020/e2294 1957 downloads

Evaluation of the immunomodulatory effect against splenocytes of Balb/c mice of biflorin obtained from Capraria biflora by a new isolation method
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Silva, Mariza S. de Lima

; Santos, Jefferson de A.

; Alves, Antônio J.

; Silva, Rosali M.F. da

; Santos, Beate S.

; Lorena, Virginia M.B. de

; Oliveira, Gibson G. de

; Melo, Cristiane M.L. de

; Góes, Alexandre J. da Silva

Revista Brasileira de Farmacognosia

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aug 2019, Volume 29 N. 4 Pages 464 - 469

Abstract: En | Text: En | PDF: En

Abstract Biflorin (6,9-dimethyl-3-(4-methylpent-3-en-1-yl) benzo[de]chromene-7,8-dione) is a promising substance that has been increasingly studied in the past decades due to its diverse pharmacological properties (i.e. antitumor, antioxidant, antiinflamatory, antimicrobial activity etc.). Aiming the comprehension of its antitumoral activity we investigated the cell proliferation and cytotoxicity habilities of biflorin against mice splenocytes Balb/c. Biflorin was able to stimulate mice splenocytes Balb/c in 48 h of incubation at a concentration of 20.2 µM. Its immunostimulation promoted the production of cytokines such as: TNF-α, IFN-γ, IL-2, IL-6 and IL-17, inducing the immune profile toward a Th1 response. Moreover, an original method which led to an excellent yield with less processing time compared to the methods described in the literature was developed to obtain biflorin, from sawdust of Capraria biflora L., Scrophulariaceae. This method shows a great potential of increasing the production of this pharmacological active compound.

https://doi.org/10.1016/j.bjp.2019.01.010 332 downloads

Brazilian legislation on genetic heritage harms Biodiversity Convention goals and threatens basic biology research and education
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ALVES, RUY JOSÉ V.

; WEKSLER, MARCELO

; OLIVEIRA, JOÃO A.

; BUCKUP, PAULO A.

; POMBAL JR., JOSÉ P.

; SANTANA, HÉLCIO R.G.

; PERACCHI, ADRIANO LÚCIO

; KELLNER, ALEXANDER W.A.

; ALEIXO, ALEXANDRE

; LANGGUTH, ALFREDO

; ALMEIDA, ALZIRA MARIA P. DE

; ALBERNAZ, ANA LUISA

; RIBAS, CAMILA C.

; ZILBERBERG, CARLA

; GRELLE, CARLOS EDUARDO V.

; ROCHA, CARLOS F.D.

; LAMAS, CARLOS JOSÉ E.

; HADDAD, CÉLIO F.B.

; BONVICINO, CIBELE R.

; PRADO, CYNTHIA P.A.

; LIMA, DANIELA O. DE

; ROSSA-FERES, DENISE C.

; SANTOS, FABRÍCIO R. DOS

; SALIMENA, FÁTIMA REGINA G.

; PERINI, FERNANDO A.

; BOCKMANN, FLÁVIO A.

; FRANCO, FRANCISCO LUÍS

; GIUDICE, GISELE M.L. DEL

; COLLI, GUARINO R.

; VIEIRA, IMA CÉLIA G.

; MARINHO-FILHO, JADER

; WERNECK, JANE M.C.F.

; SANTOS, JORGE A.D. DOS

; NASCIMENTO, JORGE LUIZ DO

; NESSIMIAN, JORGE LUIZ

; CORDEIRO, JOSÉ LUIS P.

; CLARO, KLEBER DEL

; SALLES, LEANDRO O.

; CASATTI, LILIAN

; PY-DANIEL, LUCIA HELENA R.

; SILVEIRA, LUÍS FÁBIO

; TOLEDO, LUÍS FELIPE

; OLIVEIRA, LUIZ F. DE

; MALABARBA, LUIZ ROBERTO

; SILVA, MARCELO D. DA

; COURI, MÁRCIA S.

; MARTINS, MÁRCIO

; TAVARES, MARCOS D.S.

; SOBRAL, MARCOS EDUARDO G.

; VIEIRA, MARCUS VINÍCIUS

; OLIVEIRA, MARIA DE LOURDES A.

; PINNA, MÁRIO DE

; HOPKINS, MICHAEL J.G.

; SOLÉ, MIRCO

; MENEZES, NAÉRCIO A.

; PASSOS, PAULO

; D’ANDREA, PAULO SERGIO

; PINTO, PEDRO C.E.A.

; VIANA, PEDRO L.

; TOLEDO, PETER M.

; REIS, ROBERTO E.

; VILELA, ROBERTO

; BASTOS, ROGÉRIO P.

; COLLEVATTI, ROSANE G.

; CERQUEIRA, RUI

; CASTROVIEJO-FISHER, SANTIAGO

; CARAMASCHI, ULISSES

Anais da Academia Brasileira de Ciências

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apr 2018, Volume 90 N. 2 Pages 1279 - 1284

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/0001-3765201820180460 7311 downloads

PCR-RFLP assay as an option for primary HPV test
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Golfetto, L.

; Alves, E.V.

; Martins, T.R.

; Sincero, T.C.M.

; Castro, J.B.S.

; Dannebrock, C.

; Oliveira, J.G.

; Levi, J.E.

; Onofre, A.S.C.

; Bazzo, M.L.

Brazilian Journal of Medical and Biological Research

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2018, Volume 51 N. 5 elocation e7098

Abstract: En | Text: En | PDF: En

Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for conventional cytology and another sample was collected using BD SurePath™ kit for HPV tests. Eighty samples (24.6%) were positive for HPV gene by PCR-Multiplex and were then submitted to PCR-RFLP and PapilloCheck® microarray. There was a genotyping agreement in 71.25% (57/80) on at least one HPV type between PCR-RFLP and PapilloCheck® microarray. In 22 samples (27.5%), the results were discordant and those samples were additionally analyzed by DNA sequencing. HPV 16 was the most prevalent HPV type found in both methods, followed by HPVs 53, 68, 18, 39, and 66 using PCR-RFLP analysis, and HPVs 39, 53, 68, 56, 31, and 66 using PapilloCheck® microarray. In the present study, a perfect agreement using Cohen's kappa (κ) was found in HPV 33 and 58 (κ=1), very good for HPV 51, and good for types 16, 18, 53, 59, 66, 68, 70, and 73. PCR-RFLP analysis identified only 25% (20/80) HPV coinfection, and PapilloCheck® microarray found 62.5% (50/80). Our Cohen's kappa results indicate that our in-house HPV genotyping testing (PCR-RFLP analysis) could be applied as a primary HPV test screening, especially in low income countries. If multiple HPV types are found in this primary test, a more descriptive test, such as PapilloCheck® microarray, could be performed.

https://doi.org/10.1590/1414-431x20177098 1993 downloads

Association between demyelinating disease and autoimmune rheumatic disease in a pediatric population
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Amorim, Ana Luiza M.

; Cabral, Nadia C.

; Osaku, Fabiane M.

; Len, Claudio A.

; Oliveira, Enedina M.L.

; Terreri, Maria Teresa

Revista Brasileira de Reumatologia

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may 2017, Volume 57 N. 3 Pages 224 - 228

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Introdução: Esclerose múltipla (EM) e neuromielite óptica (NMO) são doenças desmielinizantes do sistema nervoso central. A autoimunidade entre pacientes com doenças desmielinizantes e seus parentes tem sido amplamente investigada e discutida. Estudos recentes demonstram maior incidência de doenças reumáticas autoimunes entre pacientes adultos com EM e NMO e seus parentes, mas não há estudos na população pediátrica. Objetivo: Avaliar a associação de EM e NMO com doenças reumáticas autoimunes em pacientes pediátricos. Método: Foram incluídos 22 pacientes menores de 21 anos com diagnóstico de EM ou NMO antes dos 18 anos e avaliados dados epidemiológicos, clínicos, associação com doenças autoimunes, história familiar de doenças autoimunes, exames laboratoriais, exames de imagem e presença de autoanticorpos. Resultados: Entre os pacientes estudados, houve prevalência do sexo feminino (68,1%). A média de idade de início dos sintomas foi de oito anos e nove meses e a média de idade dos pacientes na avaliação foi 16 anos e quatro meses. Dois pacientes (9%) apresentaram doença reumática autoimune associada, um caso de dermatomiosite juvenil em paciente com NMO e outro de lúpus eritematoso sistêmico juvenil em paciente com EM. Três pacientes (13%) apresentaram história familiar de autoimunidade em parentes de primeiro grau. Anticorpo antinuclear (ANA) positivo foi encontrado em 80% dos pacientes com NMO e em 52% dos pacientes com EM. Cerca de 15% dos pacientes com ANA positivo apresentaram diagnóstico definitivo de doença autoimune reumática associada. Conclusão: Entre os pacientes com doenças desmielinizantes diagnosticadas durante a infância incluídos nesta pesquisa houve uma alta frequência de ANA positivo, mas uma menor taxa de associação com doenças reumáticas autoimunes do que a encontrada em trabalhos conduzidos em adultos.

ABSTRACT Introduction: Multiple sclerosis (MS) and neuromyelitis optica (NMO) are demyelinating diseases of the central nervous system. Autoimmunity in patients with demyelinating disease and in their families has been broadly investigated and discussed. Recent studies show a higher incidence of rheumatic autoimmune diseases among adult patients with MS or NMO and their families, but there are no studies in the pediatric population. Objective: To evaluate an association of MS and NMO with autoimmune rheumatic diseases in pediatric patients. Method: 22 patients younger than 21 years old with MS or NMO diagnosed before the age of 18 years were evaluated regarding epidemiological data, clinical presentation, association with autoimmune diseases, family history of autoimmune diseases, laboratory findings, imaging studies and presence of auto-antibodies. Results: Among the patients studied, there was a prevalence of females (68.1%). The mean age of symptoms onset was 8 years and 9 months and the mean current age was 16 years and 4 months. Two patients (9%) had a history of associated autoimmune rheumatic disease: one case of juvenile dermatomyositis in a patient with NMO and another of systemic lupus erythematosus in a patient with MS. Three patients (13%) had a family history of autoimmunity in first-degree relatives. Antinuclear antibody was found positive in 80% of patients with NMO and 52% of patients with MS. About 15% of antinuclear antibody-positive patients were diagnosed with rheumatologic autoimmune diseases. Conclusion: Among patients with demyelinating diseases diagnosed in childhood included in this study there was a high frequency of antinuclear antibody positivity but a lower association with rheumatologic autoimmune diseases than that observed in studies conducted in adults.

https://doi.org/10.1016/j.rbre.2016.09.016 5936 downloads

10.

The use of Eucalyptus staigeriana nanoemulsion for control of sheep haemonchosis
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Ribeiro, Wesley L.C.

; Camurça-Vasconcelos, Ana L.F.

; Santos, Jessica M.L. dos

; Macedo, Iara T.F.

; Ribeiro, Juliana de C.

; Oliveira, Erick F. de

; Paula, Haroldo C.B. de

; Bevilaqua, Claudia M.L.

Pesquisa Veterinária Brasileira

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mar 2017, Volume 37 N. 3 Pages 221 - 226

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O controle sustentável de nematoides gastrintestinais (NGI) em pequenos ruminantes tem sido baseado na utilização de métodos alternativos, incluindo o tratamento alvo-seletivo, tal como o FAMACHA. Outra alternativa de controle de NGI é o uso de plantas medicinais, embora, em muitos casos, a sua utilização seja baseada no conhecimento empírico. Nanoformulações biopoliméricas tem sido investigadas para maximizar os efeitos de óleos essenciais sobre nematoides gastrointestinais em ovinos. O objetivo do presente estudo foi combinar a nanoemulsão do óleo essencial de Eucalyptus staigeriana (EsNano) com o método FAMACHA como uma alternativa para o controle da hemoncose em ovinos. Este estudo foi realizado ao longo de seis meses em uma fazenda comercial de ovinos localizada em uma região semiárida do Nordeste do Brasil. Inicialmente, um teste de redução da contagem de ovos nas fezes (FECRT) em ovinos com levamisol, ivermectina e oxfendazole foi realizado para determinar o anti-helmíntico mais eficaz, para posterior uso como controle positivo. Levamisol foi selecionado porque mostrou eficácia superior a 95%. EsNano foi obtido e, em seguida, as suas propriedades físico-químicas foram caracterizadas. O tamanho médio (±SE) das partículas na nanoemulsão foi 276,8 (±12,3) nm, com distribuição bimodal e polidispersividade. Foram realizadas nove visitas, de abril a setembro de 2013, com um intervalo de 17 dias. Cento e sessenta e dois ovinos machos e fêmeas foram divididos em três grupos (n=54 cada) e foram tratados quando o FAMACHA foi 3, 4 ou 5: G-EsNano 250 mg kg-1 EsNano; G-Lev 7,5mg kg-1 de levamisol (controlo positivo), e G-Neg não foi tratado (controle negativo). As fezes dos ovinos foram coletadas para quantificar o número de ovos por grama de fezes (opg) e identificar os gêneros de nematóides. O ganho de peso de ovinos foi monitorado. Os dados do opg de cada grupo e os ganhos de peso médio dos ovinos foram analisados por análise de variância e comparadas com o teste de Tukey (P<0,05). Diferença significativa entre o número de animais tratados com EsNano e levamisol não foi observada em nenhuma visita (P>0,05). A variação opg foi semelhante para os grupos G-EsNano e G-Lev nas vistas (P>0,05), excetuando a segunda e quinta avaliação em que os opg dos grupos foram significativamente diferentes (P>0,05). O nematoide Haemonchus spp. foi o mais prevalente. Não houve aumento significativo de peso em qualquer um dos grupos tratados (P>0,05). Assim, a combinação de fitoterapia e FAMACHA pode ser uma alternativa para minimizar o uso anti-helmínticos sintéticos para controlar populações resistentes NGI em pequenos ruminantes.

ABSTRACT: Sustainable control of gastrointestinal nematodes (GIN) in small ruminants has been based on the use of alternative methods, including targeted selective treatment, such as FAMACHA. Another GIN control alternative is the use of herbal medicines, although in many cases their use is based on empirical knowledge. Biopolymer nanoformulations has been investigated to maximize the essential oil effects against sheep gastrointestinal nematodes. The aim of the present study was to combine a Eucalyptus staigeriana essential oil nanoemulsion (EsNano) with FAMACHA as an alternative control for sheep haemonchosis. The study was performed over six months at a commercial sheep farm located in a semiarid region of Northeast Brazil. Initially, a fecal egg count reduction test (FECRT) in sheep with levamisole, ivermectin and oxfendazole in sheep was performed used to determine the most effective anthelmintic to use as the positive control. Levamisole has been selected because it showed efficacy superior to 95%. EsNano was obtained and then its physicochemical properties were characterized. The average (±SE) size of the particles in the nanoemulsion was 276.8 (±12.3) nm with bimodal distribution and polydispersity. Nine visits were performed, from April to September 2013, with an interval of 17 days. One hundred sixty-two male and female sheep were divided into three groups (n=54 each) and were treated when FAMACHA score was 3, 4, or 5: G-EsNano 250mg kg-1 EsNano; G-Lev 7.5mg kg-1 levamisole (positive control), and G-Neg was not treated (negative control). Feces from sheep were collected to quantify the number of eggs per gram of feces (epg) and to identify nematode genera. Sheep weight gain was monitored. The epg data for each group and the average sheep weight gains were analyzed by variance analysis and compared with the Tukey’s test (P<0.05). Significant difference between the number of animals treated with EsNano and levamisole was not observed in any visit (P>0.05). The epg variation was similar in the G-EsNano and G-Lev groups on visits (P>0.05), except the second and fifth evaluation in the epg groups were significantly different (P<0.05). Haemonchus spp. was the most prevalent nematode. There was no significant weight gain in any of the treated groups (P<0.05). The combination of phytotherapy and FAMACHA can be an alternative to minimize the use of synthetic anthelmintics to control resistant GIN populations of small ruminants.

https://doi.org/10.1590/s0100-736x2017000300004 1790 downloads

11.

Association of exercise training and angiotensin-converting enzyme 2 activator improves baroreflex sensitivity of spontaneously hypertensive rats
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Lopes, P.R.

; Moreira, M.C.S.

; Marques, S.M.

; Pinto, I.S.J.

; Macedo, L.M.

; Silva, C.C.

; Freiria-Oliveira, A.H.

; Rebelo, A.C.S.

; Reis, A.A.S.

; Rosa, D.A.

; Ferreira-Neto, M.L.

; Castro, C.H.

; Pedrino, G.R.

Brazilian Journal of Medical and Biological Research

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2016, Volume 49 N. 9 elocation e5349

Abstract: En | Text: En | PDF: En

The present study sought to determine cardiovascular effects of aerobic training associated with diminazene aceturate (DIZE), an activator of the angiotensin converting enzyme 2, in spontaneously hypertensive rats (SHRs). Male SHRs (280–350 g) were either subjected to exercise training or not (sedentary group). The trained group was subjected to 8 weeks of aerobic training on a treadmill (five times a week, lasting 60 min at an intensity of 50–60% of maximum aerobic speed). In the last 15 days of the experimental protocol, these groups were redistributed into four groups: i) sedentary SHRs with daily treatment of 1 mg/kg DIZE (S+D1); ii) trained SHRs with daily treatment of 1 mg/kg DIZE (T+D1); iii) sedentary SHRs with daily treatment of vehicle (S+V); and iv) trained SHRs with daily treatment of vehicle (T+V). After treatment, SHRs were anesthetized and subjected to artery and femoral vein cannulation prior to the implantation of ECG electrode. After 24 h, mean arterial pressure (MAP) and heart rate (HR) were recorded; the baroreflex sensitivity and the effect of double autonomic blockade (DAB) were evaluated in non-anesthetized SHRs. DIZE treatment improved baroreflex sensitivity in the T+D1 group as compared with the T+V and S+D1 groups. The intrinsic heart rate (IHR) and MAP were reduced in T+D1 group as compared with T+V and S+D1 groups. Hence, we conclude that the association of exercise training with DIZE treatment improved baroreflex function and cardiovascular regulation.

https://doi.org/10.1590/1414-431x20165349 1504 downloads

12.

Ramos colaterais do Arco aórtico do preá (Galea spixii Wagler, 1831)
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Oliveira, Radan E.M.

; Oliveira, Gleidson B.

; Barbosa, Procássia M.L.

; Bezerra, Ferdinando V.F.

; Albuquerque, José Fernando Gomes de

; Ambrósio, Carlos E.

; Miglino, Maria A.

; Oliveira, Moacir F.

Pesquisa Veterinária Brasileira

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aug 2015, Volume 35 N. 8 Pages 762 - 766

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo: O preá é um roedor típico da caatinga pertencente à família Caviidae. Considerando a inexistência de dados sobre o arco aórtico do preá, foi realizado este estudo tendo como objetivo descrever os ramos colaterais do arco aórtico neste cavídeo, e dessa forma, contribuir com dados para biologia da espécie. Foram utilizados vinte preás machos provenientes de estudos anteriores e encontravam-se armazenados em freezer no Centro de Multiplicação de Animais Silvestres (CEMAS/UFERSA). Os animais foram descongelados, a cavidade torácica foi aberta, a aorta canulada e o sistema vascular lavado com solução salina e em seguida, injetado látex Neoprene corado com pigmento vermelho, amarelo ou branco. Posteriormente, os animais foram fixados em formol e depois de 72 horas, dissecados e analisados, sendo obtidos desenhos esquemáticos e os exemplares mais representativos fotografados. O arco aórtico do preá emitiu como ramos colaterais, o tronco braquiocefálico e a artéria subclávia esquerda. O tronco braquiocefálico originou na maioria das peças estudadas, a artéria carótida comum esquerda e o tronco braquiocarotídeo, do qual surgem as artérias subclávia direita e carótida comum direita. As artérias subclávias direita e esquerda em todos os animais estudados emitiram a artéria vertebral, a artéria torácica interna, a artéria cervical superficial, o tronco costocervical e a artéria axilar. O padrão da formação do arco aórtico do preá assemelhou-se ao observado em outros roedores, tais como no mocó, no porquinho-da-índia e na chinchila.

Abstract: Galea spixii is a typical rodent of the caatinga belonging to the Caviidae family. Considering the lack of data on the aortic arch of the galea, this study aimed to describe the collateral branches of the aortic arch in this specie and to contributes to knowledge of its biology. Twenty males from previous studies were used which had been stored in a freezer at Multiplication Center of Wild Animals (CEMAS/UFERSA). The animals were thawed, the thoracic cavity was opened and the aorta cannulated vasculature was washed with saline and then injected with Neoprene latex colored with red pigment, yellow or white. Subsequently, the animals were fixed in formalin and after 72 hours dissected and analyzed, to obtain schematic drawings and the most representative samples were photographed. The cavy aortic arch issued as collateral branches, the brachiocephalic trunk and the left subclavian artery. The brachiocephalic trunk afforded in most of the specimens the left common carotid artery and the brachiocarotid trunk, where the right subclavian and right common carotid arteries arise. The right and left subclavian arteries in all animals studied issued the vertebral artery, internal thoracic artery, superficial cervical artery costocervical trunk and axillary artery. The pattern of formation of the aortic arch of galea was similar to that observed in other rodents, such as the kerodon, the guinea pig and chinchilla.

https://doi.org/10.1590/S0100-736X2015000800008 1699 downloads

13.

Determinação da concentração alveolar mínima do isofluorano em catetos (Tayassu tajacu)
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Oliveira, Maria G.C.

; Morais, Alessandro M.L.

; Lima, Amara G.A.

; Paiva, Ariana L.C.

; Nunes, Talyta L.

; Passos, Yanna D.B.

; Oliveira, Moacir F.

; Paula, Valéria V.

Pesquisa Veterinária Brasileira

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jun 2014, Volume 34 N. 6 Pages 576 - 581

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A anestesia inalatória vem sendo amplamente difundida na medicina veterinária, no entanto seu uso em animais selvagens ainda é restrito, não sendo observado nenhum estudo referente à sua utilização na espécie Tayassu tajacu. O objetivo da pesquisa foi determinar a concentração alveolar mínima (CAM) do isofluorano em catetos e apresentar os efeitos desta administração sobre as variáveis hemodinâmicas e respiratórias, como também a qualidade da recuperação anestésica. Utilizou-se 10 animais, machos, com idade variando de 1 a 3 anos oriundos do Centro de Multiplicação de Animais Silvestres da Universidade Federal Rural do Semi-Árido, Brasil. Todos os animais tiveram anestesia induzida com 7mg.kg-1 de propofol e posteriormente foram conectados a circuito anestésico com isofluorano e oxigênio 100%. O estímulo noceptivo supramáximo adotado foi pinçamento interdigital, o qual era realizado após 15 minutos de espera para cada concentração de isofluorano fornecida. Ao ser observada resposta negativa frente ao estímulo a concentração era reduzida em 20%, quando verificada resposta positiva o estímulo era cessado, calculando-se a partir daí o valor da CAM. Observou-se dados quantitativos e qualitativos referentes à recuperação. Utilizou-se o teste de normalidade de Shapiro Wilk e de homogeneidade de variânica de Levene, as variáveis avaliadas foram submetidas à One Way ANOVA-RM para medidas repetidas, seguidas por Teste Tukey, sendo os dados expressos em média e desvio padrão. A CAM do isofluorano foi de 2,4%, sendo a CAM cirúrgica igual a 3,5%. Observou-se ação depressiva do isofluorano sobre a pressão arterial, frequência cardíaca e respiratória quando comparada a média dessas variáveis para animais acordados, entretanto durante a manutenção anestésica mantiveram-se estáveis. Observou-se acidose metabólica no período pré-anestésico o qual foi compensado após a realização da anestesia inalatória. A recuperação anestésica foi tranquila e rápida. Concluiu-se que a CAM do isofluorano para catetos foi maior que a observada em espécies afins. O isofluorano pode ser utilizado nesta espécie, sendo considerado seguro e eficaz. A recuperação dos animais após anestesia com isofluorano foi livre de excitação.

Inhalation anesthesia has been widespread in veterinary medicine. Nevertheless, its use in wild animals is still limited, having no studies on its use been observed in the species. The objective of the research was to determine the isoflurane minimum alveolar concentration (MAC) in peccaries and present the effects of its administration on the hemodynamic and respiratory variables, as well as data concerning the anesthesia recovery. The study used 10 male animals with age ranging from one to three years, from the Centro de Multiplicação de Animais Silvestres of Universidade Federal Rural do Semi-Árido, Brazil. All the animals had anesthesia induced with propofol 7mg.kg-¹, were intubated and connected to the anesthetic circuit with isoflurane and 100% oxygen. The supramaximal noxious stimulation used was the interdigital pinch, which was performed after 15 minutes of waiting for each provided isoflurane concentration. When negative response to the stimulus was observed, the concentration was reduced by 20%; when positive response was verified, the stimulus was stopped, being the CAM value calculated from that point. Quantitative and qualitative data regarding recovery were also observed. Shapiro-Wilk test for normality was performed, as well as Levene test for equality of variance; the variables were subjected to One Way RM ANOVA for repeated measures followed by Tukey test, and the data expressed as mean and standard deviation. The MAC of isoflurane was 2.4% and the surgical MAC equal to 3.5%. Depressive action of isoflurane on blood pressure, heart and breathing rates was observed when compared to the average of the variables on awake animals; however, during maintenance of anesthesia, they remained stable. Metabolic acidosis was observed in the pre-anesthetic, which was offset after inhalation anesthesia. Recovery was quiet and smooth. It was concluded that the isoflurane MAC for peccaries was greater than that observed in related species. Isoflurane can be used in this species, being considered safe and effective. The animals' recovery after anesthesia with isoflurane was free from excitement.

4532 downloads

14.

Níveis de proteína na dieta de novilhas da raça Pantaneira: desempenho e digestibilidade aparente
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Teodoro, A.L.

; Oliveira, M.V.M.

; Vargas Junior, F.M.

; Juliano, R.S.

; Longo, M.L.

; Seno, L.O.

; Oliveira, C.A.L.

Archivos de Zootecnia

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sep 2013, Volume 62 N. 239 Pages 369 - 378

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

The objective of this experiment was to verify the influence of increasing levels of dietary protein on performance, apparent digestibility and plasma and urinary urea in Pantaneira breed. The data were obtained from 15 heifers, 18 months old and 146 kg initial body weight, fed diets containing 11, 13, 15 and 17 % of crude protein (CP). The animals were confined for 114 days. At intervals of 28 days they were weighed and blood samples and spot samples of feces from the rectum and of urine were taken. The increase in dietary CP content did not influenced dry matter (DM) intake and average daily weight gain (ADG), but improved nutrient digestibility, increased blood nitrogen levels and increased excretion of urinary urea. A mean intake of 5.13 kg DM/animal/day and an ADG of 570 g were observed. Apparent digestibility values of 40.9, 59.6, 39.2, 41.3 and 34,0 % for DM, CP, total carbohydrate, neutral detergent fiber and acid detergent fiber, respectively, were found. Plasma urea concentration was 49.6 mg/dL; and an urinary urea loss of 110,0 g/animal/day was observed.

Objetivou-se com este trabalho verificar a influência de níveis crescentes de proteína dietética sobre o desempenho, digestibilidade aparente e concentrações de uréia plasmática e urinária, em bovinos da raça Pantaneira. Os dados foram obtidos de 15 novilhas, com 18 meses de idade e peso corporal inicial de 146 kg, recebendo dietas com 11; 13; 15 e 17 % de proteína bruta (PB). Os animais permaneceram confinados por 114 dias e em intervalos de 28 dias foram pesados e realizadas coletas de sangue, de fezes na ampola retal e de urina na forma spot. O aumento do teor de PB não influenciou o consumo de matéria seca (MS) e o ganho de peso médio diário (GMD); porém melhorou a digestibilidade dos nutrientes, elevou os níveis de nitrogênio no sangue e promoveu maior excreção de uréia urinária. Observou-se, em média, consumos de 5,13 kg MS/animal/dia; GMD de 570 g; coeficientes de digestibilidade aparente de 40,9; 59,6; 39,2; 41,3 e 34,0 % para MS, PB, carboidrato total (CT), fibra detergente neutro (FDN) e ácido (FDA), respectivamente; concentração de uréia plasmática de 49,6 mg/dL; e perda de uréia urinária de 110,0 g/animal/dia.

https://doi.org/10.4321/S0004-05922013000300005 512 downloads

15.

Effects of irrigated rice sowing season and imazapyr + imazapic time of application on rice grain yield and red rice management
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Sartori, G.M.S.

; Marchesan, E.

; Azevedo, C.F.

; Filho, A.C.

; Roso, R.

; Coelho, L.L.

; Oliveira, M.L.

Planta Daninha

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sep 2013, Volume 31 N. 3 Pages 631 - 644

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A época de semeadura de arroz irrigado e a competição com arroz-vermelho estão entre os principais fatores que alteram o rendimento de grãos da cultura. O objetivo deste estudo foi avaliar épocas de semeadura do arroz irrigado e dos momentos de aplicação do herbicida imazapyr+imazapic no controle de arroz-vermelho e no rendimento de grãos de arroz irrigado. Realizaram-se oito experimentos nas seguintes épocas: (30/09, 19/10, 08/11 e 01/12) safra 2010/11 e (27/09, 17/10, 08/11 e 05/12) safra 2011/12. Os tratamentos avaliados foram: aplicação do herbicida imazapyr+imazapic nas doses de 105+35 g ha-1 em pré-emergência (PRE); 52,5+17,5 g ha-1 em pré-emergência e 52,5+17,5 g ha-1 em pós-emergência (PRE+POS); e 105+35 g ha-1 em pós-emergência (POS), além de uma testemunha sem aplicação e sem capina. O cultivar utilizado foi Puitá Inta CL, e o delineamento utilizado foi blocos ao acaso em quatro repetições. Realizou-se análise conjunta dos experimentos em cada safra. Ocorreu menor emergência de arroz-vermelho e maior rendimento de grãos de arroz irrigado no início da época (30/09/10 e 27/09/11), com 10.578 e 8.653 kg ha-1, respectivamente. No final da época (01/12/10 e 05/12/11), houve maior redução do banco de sementes do arroz-vermelho. A semeadura no início da época recomendada proporciona maior rendimento de grãos de arroz irrigado. A aplicação de imazapyr+imazapic nas doses de 52,5+17,5 g ha-1 em PRE + 52,5+17,5 g ha-1 POS e de 105+35 g ha-1 somente em PRE e POS foi eficiente no controle do arroz‑vermelho.

Irrigated rice sowing season and red rice competition are among the main factors affecting grain yield. The objective of this work was to evaluate the sowing date of irrigated rice and moments of application of the herbicide imazapyr + imazapic to control red rice management and irrigated rice grain yield. Eight experiments were performed at the following dates (09/30, 10/19, 11/08 and 12/01) for the 2010/2011 harvest season and (09/27, 10/17, 11/08 and 12/05) for the 2011/2012 harvest season. The treatments were: application of the herbicide imazapyr + imazapic at doses of 105+35 g ha-1 in pre-emergence (PRE); 52.5+17.5 g ha‑1 in pre-emergence and 52.5+17.5 g ha-1 in post-emergence (PRE + POST); and 105+35 g ha-1 in post- emergence (POST), and a control without application and no weeding. The cultivar Puitá Inta CL was used and a randomized block design with four replicates. A joint analysis of the experiments was carried out. There was less emergence of red rice and higher grain yield of the irrigated rice at the early periods (09/30/10 and 09/27/11), with 10,578 and 8,653 kg ha-1, respectively. At the end of the season (12/01/10 and 12/05/11), there was greater reduction of the red rice seed bank. Sowing at the beginning of the recommended period provided more irrigated rice grain yield. The application of imazapyr + imazapic at a dose of 52.5+17.5 g ha-1 in PRE + 52.5+17.5 g ha-1 POST, and 105+35 g ha-1 only in PRE and POST was effective in the control of red rice.

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You can enrich your search in a very simple way. Use the search indexes combined with the connectors (AND or OR) and specify more your search.

For example, if you want to search for articles about
cases of dengue in Brasil in 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

See below the complete list of search indexes that can be used:

Index code	Element
ti	article title
au	author
kw	article keywords
subject	subject (title words, abstract and keywords)
ab	abstract
ta	journal short title (e.g. Cad. Saúde Pública)
journal_title	journal full title (e.g. Cadernos de Saúde Pública)
la	publication language code (e.g. pt - Portuguese, es - Spanish)
type	document type
pid	publication identifier
publication_year	publication year of publication
sponsor	sponsor
aff_country	country code of the author's affiliation
aff_institution	author affiliation institution
volume	article volume
issue	article issue
elocation	elocation
doi	DOI number
issn	journal ISSN
in	SciELO colection code (e.g. scl - Brasil, col - Colômbia)
use_license	article usage license code

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