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ABSTRACT BACKGROUND: We aimed to describe the clinical characteristics of coronavirus disease 2019 (COVID-19) among healthcare workers (HCWs) in Curitiba, Brazil. METHODS: Upper respiratory samples from 1077 HCWs were tested for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using reverse transcription polymerase chain reaction from June 16, 2020 to December 9, 2020. RESULTS: Overall, 32.7% of HCWs were infected. The positivity rates in symptomatic and asymptomatic HCWs were 39.2% and 15.9%, respectively. Hospital departments categorized as high-risk for exposure had the highest number of infected HCWs. CONCLUSIONS: Early diagnosis and isolation of infected HCWs remain key in controlling SARS-CoV-2 transmission because HCWs in close contact with COVID-19 patients are more likely to be infected than those who are not.

https://doi.org/10.1590/0037-8682-0265-2021

Influenza Sentinel Surveillance and Severe Acute Respiratory Infection in a Reference Hospital in Southern Brazil
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Faggion, Heloisa Zimmerman

; Leotte, Jaqueline

; Trombetta, Hygor

; Pereira, Luciane Aparecida

; Lapinski, Bruna Amaral

; Nogueira, Meri Bordignon

; Vidal, Luine Rosele

; Almeida, Bernardo Machado

; Petterle, Ricardo Rasmussen

; Raboni, Sonia Mara

Revista da Sociedade Brasileira de Medicina Tropical

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2020, Volume 53 elocation e20170498

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION: We report the results of the active surveillance of influenza infections in hospitalized patients and the evaluation of the seasonality and correlation with temperature and rainfall data. METHODS: During the 2-year study period, 775 patients were tested for 15 respiratory viruses (RVs). RESULTS: Most of the 57% of (n=444) virus-positive samples were human rhinovirus and respiratory syncytial virus. However, 10.4% (n=46) were influenza virus (80% FluA; 20% FluB). Age and SARI were significantly associated with influenza. FluB circulation was higher is 2013. CONCLUSIONS: In the post-epidemic period, influenza remains an important cause of hospitalization in SARI patients.

https://doi.org/10.1590/0037-8682-0498-2017 1428 downloads

Human parechovirus: sepsis-like illness with pulmonary infection
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Vidal, Luíne Rosele Renaud

; Cavalli, Bárbara

; Almeida, Sérgio Monteiro de

; Raboni, Sônia Mara

; Nogueira, Meri Bordignon

Brazilian Journal of Infectious Diseases

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dec 2017, Volume 21 N. 6 Pages 675 - 677

Abstract: En | Text: En | PDF: En

https://doi.org/10.1016/j.bjid.2017.06.004 709 downloads

Profile of HIV subtypes in HIV/HBV- and HIV/HCV-coinfected patients in Southern Brazil
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Avanzi, Valéria Miranda

; Vicente, Bianca Arão

; Beloto, Nayara Carvalho Polido

; Gomes-da-Silva, Monica Maria

; Ribeiro, Clea Elisa Lopes

; Tuon, Felipe Francisco

; Vidal, Luine Rosele Renaud

; Nogueira, Meri Bordignon

; Raboni, Sonia Mara

Revista da Sociedade Brasileira de Medicina Tropical

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aug 2017, Volume 50 N. 4 Pages 470 - 477

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION: HIV and viral hepatitis infections are major causes of chronic disease worldwide and have some similarities with regard to routes of transmission, epidemiology, front barriers faced during access of treatment, and strategies for a global public health response. The objective was to describe the HIV-1 subtypes, viral tropism and single-nucleotide polymorphisms (SNPs) of interleukin 28B (IL28B) from a case series of HIV/viral hepatitis coinfected patients from southern Brazil. METHODS: Clinical and epidemiological data were evaluated by a review of medical records. Periodic blood draws were taken to determine the viral and host characteristics. RESULTS: This study included 38 patients with HIV/HBV or HIV/HCV coinfection; the median age was 49 years. Thirty-seven (97.4%) were on antiretroviral therapy, 32 (84.2%) had an undetectable viral load, a median CD4+ T-cell count of 452 cells/mm3. HIV-1 subtyping showed 47.4 and 31.6% of patients with subtypes C and B, respectively. Analysis of viral co-receptor usage showed a predominance of the R5 variant (64.7%), with no significant difference between the subtypes. Twenty patients with HIV/HCV coinfection were eligible to receive HCV therapy with pegylated-interferon-alpha plus ribavirin, and 10/20 (50%) of them achieved sustained virological response. SNPs of IL28B were evaluated in 93.3% of patients with HIV/HCV coinfection, and 17 (60.7%) presented the CC genotype. CONCLUSIONS: In the present case series, a higher frequency of HIV subtype C was found in coinfected patients. However such findings need to be prospectively evaluated with the inclusion of data from regional multicenter analyses.

https://doi.org/10.1590/0037-8682-0450-2016 1405 downloads

Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR
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Almeida, Sérgio Monteiro de

; Raboni, Sônia Mara

; Nogueira, Meri Bordignon

; Vidal, Luine R. Renaud

Arquivos de Neuro-Psiquiatria

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oct 2016, Volume 74 N. 10 Pages 810 - 815

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A presença de hemoglobina em amostras de fluidos corporais é considerada um fator inibitório importante da reação em cadeia da polimerase (PCR). O objetivo deste estudo era examinar a influencia de hemácias no líquido cefalorraquidiano (LCR) como um fator inibitório da RT-PCR para enterovirus (EV). Quatrocentos e quarenta amostras de LCR de pacientes com características de meningite viral foram avaliados para enterovirus por RT-PCR. RNA viral foi extraído com tampão de isotiocianato de guanidina e a detecção viral foi feita com nested PCR in-house. A positividade do EV RT-PCR no LCR foi maior nas amostras de LCR sem hemácias do que as amostras com hemácias: 13 (26%) e 36 (9,2%), respectivamente (p = 0,001). No grupo com resultados EV RT-PCR positivo, a media ± DP do número de hemácias no LCR foi 37 ± 183 cell/mm3 e no grupo com resultados negativos foi 580 ± 2.890 cell/mm3 (p = 0,007). O limite superior aceitável de hemácias no LCR para não inibir o resultado do PCR foi 108 cells/mm3. As amostras de LCR com resultados negativos para RT-PCR EV tem mais eritrócitos em comparação com amostras com resultados positivos.

ABSTRACT The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics of viral meningitis were assessed for EV by RT-PCR. Viral RNA extracted with guanidine isothyocianate buffer and virus detection was performed by in-house nested PCR. Positivity for EV RT-PCR was higher in CSF samples without RBCs than in samples with RBCs: 13(26%) and 36(9.2%), p = 0.001. In the group with positive EV RT-PCR, the mean + SD CSF RBC was 37 ± 183 cell/mm3; the group with negative results had 580 + 2,890 cell/mm3 (p = 0.007). The acceptable upper limit for CSF RBCs that could not influence RT-PCR was 108 cells/mm3. CSF samples with negative results for EV RT-PCR have more erythrocytes.

https://doi.org/10.1590/0004-282X20160119 4352 downloads

Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients
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Ferreira, Carla Elizabeth de Oliveira

; Raboni, Sonia Mara

; Pereira, Luciane Aparecida

; Nogueira, Meri Bordignon

; Vidal, Luine Rosele Renaud

; Almeida, Sérgio Monteiro

Brazilian Journal of Infectious Diseases

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jun 2012, Volume 16 N. 3 Pages 267 - 272

Abstract: En | Text: En | PDF: En

BACKGROUND: Acute gastroenteritis (AGE) is a common disorder that affects children worldwide. It is usually caused by viral agents, including rotavirus, enteric adenovirus, norovirus, and astrovirus groups. Currently, there are few reports about co-infection among these viruses, mainly in Brazil. METHODS: This is a retrospective study in which 84 rotavirus-positive samples from hospitalized patients at a teaching hospital in Southern Brazil, collected in the 2001-2010 period, were analyzed by polymerase chain reaction (PCR) and reverse transcription - polymerase chain reaction (RT-PCR), for the investigation of enteric adenovirus, astrovirus, and norovirus. RESULTS: In total, 12 of the 84 (14%) samples were positive to enteric adenovirus or norovirus. Clinical, laboratory, and demographic data showed statistically significant differences between mono and co-infected patients, including age and depletion rate. CONCLUSIONS: These findings highlight the need for implementation of other enteric virus detection assays in clinical diagnosis for a complete laboratory investigation of hospitalized pediatric patients with AGE, in order to understand the impact of these pathogens on disease severity, spread within hospital, and consequently, prevent the dissemination of nosocomial infections.

7152 downloads

Enterovirus and herpesviridae family as etiologic agents of lymphomonocytary meningitis, Southern Brazil
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Vidal, Luine Rosele Renaud

; Almeida, Sérgio Monteiro de

; Messias-Reason, Iara José de

; Nogueira, Meri Bordignon

; Debur, Maria do Carmo

; Pessa, Luís Felipe Cavalli

; Pereira, Luciane Aparecida

; Rotta, Indianara

; Takahashi, Gislene Reche de Almeida

; Silveira, Clyete Santos da

; Araújo, Josianne Maria Reimann

; Raboni, Sonia Mara

Arquivos de Neuro-Psiquiatria

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jun 2011, Volume 69 N. 3 Pages 475 - 481

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A meningite viral é uma síndrome infecciosa comum do sistema nervoso central (SNC), que ocorre no mundo inteiro. O objetivo deste estudo foi identificar o agente etiológico de meningite linfomonocitária em Curitiba, PR, Brasil. Durante o período de julho de 2005 a dezembro de 2006, 460 amostras com meningite linfomonocitária foram analisadas por metodologias de PCR. Cinquenta e nove (12,8%) amostras foram positivas. Enterovirus estava presente em 49 (83%) amostras e herpes vírus em 10 (17%), destas 6 (10%) HSV, 1 (2%) EBV, 2 (3%) HHV- 6 e 1 (2%) infecção mista de enterovírus e EBV. Conclui-se que o enterovirus foi o vírus mais frequente, com a circulação durante o verão. Houve maior número de amostras positivas entre 4 a 17 anos. A metodologia de PCR é um importante método para a detecção rápida de RNA de enterovirus e DNA do herpesvirus no LCR.

Viral meningitis is a common infectious disease of the central nervous system (CNS) that occurs worldwide. The aim of this study was to identify the etiologic agent of lymphomonocytary meningitis in Curitiba, PR, Brazil. During the period of July 2005 to December 2006, 460 cerebrospinal fluid (CSF) samples with lymphomonocytary meningitis were analyzed by PCR methodologies. Fifty nine (12.8%) samples were positive. Enteroviruses was present in 49 (83%) samples and herpes virus family in 10 (17%), of these 6 (10%) herpes simplex virus, 1 (2%) Epstein Barr virus, 2 (3%) human herpes virus type 6 and 1 (2%) mixed infection of enterovirus and Epstein Barr virus. As conclusion enterovirus was the most frequent virus, with circulation during summer and was observed with higher frequency between 4 to 17 years of age. PCR methodology is an important method for rapid detection of RNA enterovirus and DNA herpesvirus in CSF.

4997 downloads

Molecular epidemiology of HIV-1 clades in Southern Brazil
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Raboni, Sonia Mara

; Almeida, Sérgio Monteiro de

; Rotta, Indianara

; Ribeiro, Cléa Elisa Lopes

; Rosario, Debra

; Vidal, Luine Rosele

; Nogueira, Meri Bordignon

; Riedel, Maristela

; Winhescki, Maria da Graça

; Ferreira, Kátia Antunes

; Ellis, Ronald

Memórias do Instituto Oswaldo Cruz

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dec 2010, Volume 105 N. 8 Pages 1044 - 1049

Abstract: En | Text: En | PDF: En

Human immunodeficiency virus (HIV) clades B and C account for more than 60% of the HIV-1 infections worldwide. In this paper, we describe the profiles of patients infected with subtypes of HIV-1 from the state of Paraná, Southern Brazil, and correlate them with demographic and epidemiological findings. A retrospective analysis of HIV cases reported from 1999-2007 was also performed. Data from 293 patients were reviewed and 245 were older than 13 (58% female). The distribution of clades was as follows: B 140 (57%), C 67 (23%), F 24 (10%) and mosaic or unique recombinant forms (URFs) 24 (10%). Of the 48 patients younger than 13 years of age (62.5% male), vertical transmission occurred in 46 and the distribution of clades was as follows: B 14 (29%), C 24 (50%), F 7 (15%) and URFs 6 (13%). There was no significant difference in mortality between HIV-1 subtypes. In both groups, patients infected with clade C tended to have higher rates of injection drug use exposure risk.

2144 downloads Cited 2 times in SciELO

Comparison of immunoperoxidase and immunofluorescence assays for pp65 cytomegalovirus antigen in immunocompromised patients
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Debur, Maria do Carmo

; Vidal, Luine R. Rosele

; Nogueira, Meri Bordignon

; Pereira, Luciane Aparecida

; Takahashi, Gislene R.A.

; Rotta, Indianara

; Almeida, Sérgio Monteiro de

; Raboni, Sonia Mara

Brazilian Journal of Infectious Diseases

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apr 2009, Volume 13 N. 2 Pages 142 - 146

Abstract: En | Text: En | PDF: En

We compared the pp65 antigen detection by an in house method (immunoperoxidase assay) and by a commercial kit (immunofluorescence assay) available for cytomegalovirus infection diagnosis in immunocompromised patients. Sixty-four blood samples were analyzed in duplicate for both techniques. Eight-six percent of the samples had concordant qualitative results. The discordant results occurred more frequently in samples with low quantity of positive cells. There were no significant differences with qualitative and quantitative results of the methods.

2309 downloads

10.

Evidence for the co-circulation of dengue virus type 3 genotypes III and V in the Northern region of Brazil during the 2002-2004 epidemics
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Nogueira, Meri Bordignon

; Stella, Vanessa

; Bordignon, Juliano

; Batista, Weber Cheli

; Borba, Luana de

; Silva, Luis Hildebrando Pereira da

; Hoffmann, Federico Guillermo

; Probst, Christian Macagnan

; Santos, Claudia Nunes Duarte dos

Memórias do Instituto Oswaldo Cruz

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aug 2008, Volume 103 N. 5 Pages 483 - 488

Abstract: En | Text: En | PDF: En

The reintroduction of dengue virus type 3 (DENV-3) in Brazil in 2000 and its subsequent spread throughout the country was associated with genotype III viruses, the only DENV-3 genotype isolated in Brazil prior to 2002. We report here the co-circulation of two different DENV-3 genotypes in patients living in the Northern region of Brazil during the 2002-2004 epidemics. Complete genomic sequences of viral RNA were determined from these epidemics, and viruses belonging to genotypes V (Southeast Asia/South Pacific) and III were identified. This recent co-circulation of different DENV-3 genotypes in South America may have implications for pathological and epidemiological dynamics.

2814 downloads Cited 5 times in SciELO

11.

Laboratorial diagnosis of lymphocytic meningitis
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Almeida, Sérgio Monteiro de

; Nogueira, Meri Bordignon

; Raboni, Sonia Mara

; Vidal, Luine Rosele

Brazilian Journal of Infectious Diseases

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oct 2007, Volume 11 N. 5 Pages 489 - 495

Abstract: En | Text: En | PDF: En

Meningitis is the main infectious central nervous system (CNS) syndrome. Viruses or bacteria can cause acute meningitis of infectious etiology. The term "Aseptic Meningitis" denotes a clinical syndrome with a predominance of lymphocytes in the cerebrospinal fluid (CSF), with no common bacterial agents identified in the CSF. Viral meningitis is considered the main cause of lymphocyte meningitis. There are other etiologies of an infectious nature. CSF examination is essential to establish the diagnosis and to identify the etiological agent of lymphocytic meningitis. We examined CSF characteristics and the differential diagnosis of the main types of meningitis.

68699 downloads Cited 3 times in SciELO

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