RESUMO Salmonella Gallinarum pode causar alta mortalidade em aves da ordem Galliformes. Objetivou-se neste estudo relacionar a presença de sinais clínicos com a recuperação de Salmonella Gallinarum de órgãos e swabs cloacais de codornas japonesas (Coturnix coturnix) experimentalmente infectadas. Um total de 70 codornas fêmeas foram alojadas em par por gaiola e divididas em dois grupos (IG: codornas inoculadas com 1,5x106 UFC de Salmonella Gallinarum Nalr / mL e CG: grupo controle). Após a inoculação, as aves foram avaliadas três vezes ao dia para se verificar a presença de sinais clínicos. As aves que se apresentaram com penas eriçadas, olhos fechados e permaneciam imóveis na gaiola foram removidas para a eutanásia, assim como o mesmo número de aves dos grupos inoculados que não apresentaram sinais clínicos e do grupo controle. O swab cloacal foi realizado após a eutanásia para a amostragem de fígado, baço, ceco, folículos ovarianos e pulmão para procedimento microbiológico. As codornas com sinais clínicos e as encontradas mortas apresentaram positividade de 100%, enquanto as codornas inoculadas sem sinais clínicos apresentaram menor positividade (38,5%). Portanto, codornas com septicemia causada por SG apresentam sinais clínicos da doença e o patógeno pode ser isolado e quantificado em diversos órgãos.

ABSTRACT Salmonella Gallinarum is capable of causing high mortality in birds of the order Galliformes. This study aimed to relate the presence of clinical signs with the recovery of Salmonella Gallinarum from organs and c loacal swabs of Japanese quails (Coturnix coturnix) experimentally infected. A total of 70 female quails were housed in a pair per cage and divided in two groups (IG: quails inoculated with 1.5x106 CFU of Salmonella Gallinarum Nalr/mL and CG: control group). After the inoculation, birds were evaluated three times a day to verify the presence of clinical signs. Birds that presented ruffled feathers, eyes closed and remained quiet in the cage were removed for euthanasia, as well as the same number of birds from the inoculated groups that presented no clinical signs and from the control group. Cloacal swabbing was performed following euthanasia for the sampling of liver, spleen, caeca, ovarian follicles and lung for microbiological procedure. Quails with clinical signs and quails found dead presented positivity of 100%. While inoculated quails with no clinical signs presented a lower positivity (38.5%). Therefore, quails with septicemia caused by SG present clinical signs of the disease and the pathogen can be isolated and quantified in the organs.

ABSTRACT Yellow sweet potato is mostly produced by small farmers, and may be a source of energy and carotenoids in the human diet, but it is a highly perishable crop. To increase its industrial application, yellow sweet potato flour has been produced for use in bakery products. This study aimed to evaluate the technological quality and the carotenoids content in sweet breads produced with the replacement of wheat flour by 0, 3, 6, and 9% yellow sweet potato flour. Breads were characterized by technological parameters and β-carotene levels during nine days of storage. Tukey’s test (p<0.05) was used for comparison between means. The increase in yellow sweet potato flour concentrations in bread led to a decrease of specific volume and firmness, and an increase in water activity, moisture, orange coloring, and carotenoids. During storage, the most significant changes were observed after the fifth day, with a decrease in intensity of the orange color. The β-carotene content was 0.1656 to 0.4715 µg/g in breads with yellow sweet potato flour. This work showed a novel use of yellow sweet potato in breads, which brings benefits to consumers’ health and for the agricultural business.

OBJECTIVE: The aim of this study was to evaluate the roles of the Taql and Bsml vitamin D receptor gene polymorphisms in hospital mortality of burn patients. METHODS: In total, 105 consecutive burn injury patients over 18 years in age who were admitted to the Burn Unit of Bauru State Hospital from January to December 2013 were prospectively evaluated. Upon admission, patient demographic information was recorded and a blood sample was taken for biochemical analysis to identify the presence of the Taql(rs731236) and Bsml(rs1544410) polymorphisms. All of the patients were followed over their hospital stay and mortality was recorded. RESULTS: Eighteen of the patients did not sign the informed consent form, and there were technical problems with genotype analysis for 7 of the patients. Thus, 80 patients (mean age, 42.5±16.1 years) were included in the final analysis. In total, 60% of the patients were male, and 16.3% died during the hospital stay. The genotype frequencies for the Taql polymorphism were 51.25% TT, 41.25% TC and 7.50% CC; for the Bsml polymorphism, they were 51.25% GG, 42.50% GA and 6.25% AA. In logistic regression analysis, after adjustments for age, gender and total body surface burn area, there were no associations between the Taql (OR: 1.575; CI95%: 0.148-16.745; p=0.706) or Bsml (OR: 1.309; CI95%: 0.128-13.430; p=0.821) polymorphisms and mortality for the burn patients. CONCLUSIONS: Our results suggest that the Taql and Bsml vitamin D receptor gene polymorphisms are not associated with hospital mortality of burn patients.

Objetivou-se avaliar duas fontes de amido (milho ou sorgo) no concentrado e três classes sexuais sobre o consumo de nutrientes, o desempenho e as características da carcaça de bovinos superprecoces. Foram utilizados 30 animais cruzados Beefalo-Nelore, com peso corporal médio inicial de 234,38±26,17kg, em delineamento inteiramente ao acaso, em esquema fatorial 2x3 (duas fontes de amido e três classes sexuais: fêmeas, machos castrados e machos não castrados). O período experimental foi de 224 dias. Na fase inicial (zero-17 semanas), os ganhos de peso total (GPT) e diário (GMD) foram 150,62 e 108,62kg e 1,27 e 0,91kg/dia, respectivamente, para milho e sorgo. Na fase final (17-32 semanas), houve efeito da fonte de amido para o peso de carcaça, com médias de 273,00 e 231,95kg, respectivamente, para os tratamentos com milho e sorgo. Houve efeito de classe sexual para GP, GMD e eficiência alimentar, com médias de GP iguais a 98,17, 124,70 e 119,70kg, GMD de 0,93, 1,19 e 1,14kg/dia e 8,66, 12,24 e 11,09%, respectivamente, para fêmeas, machos castrados e machos não castrados. Espessura de gordura subcutânea (EGS) apresentou médias de 10,50; 5,97 e 5,51mm, respectivamente, para fêmeas, machos castrados e machos não castrados. Recomenda-se o milho como fonte de amido na fase inicial do confinamento de superprecoces. Na fase final (17-32 semanas), não há distinção entre milho ou sorgo. As fêmeas apresentaram maior EGS, e os machos, castrados e não castrados, apresentaram maior eficiência alimentar na fase final do confinamento.

The aim of this study was to evaluate two sources of starch in the concentrate, and three sex classes, on the consumption of nutrients, the performance and carcass characteristics of young bovines in feedlot. Thirty Beefalo-Nellore crossbred with an average initial body weight of 234.38±26.17kg and average age of nine months was used. All animals were maintained in individual bays. A completely randomized design with 2 x 3 factorial (two starch source and three sexual categories) was used. The experimental period lasted 224 days (32 weeks). In the initial phase (0-17 weeks) the weight gain (WG) and average daily gain (ADG) were 150.62 and 108.62kg and 1.27 and 0.91kg/day, respectively, for corn and sorghum. In the final phase (17-32 weeks) the feed efficiency of animals fed with maize was similar to sorghum, with an average of 10.66%. There was a significant source of starch for carcass weight, averaging 273.00 and 231.95kg respectively for the treatments with maize and sorghum. There was a sex class effect for WG, ADG and feed efficiency, with WG average equal to 98.17, 124.70 and 119.70kg, average daily gain of 0.93, 1.19 and 1.14kg/day and 8.66, 12.24 and 11.09% respectively for heifers, steers and young bulls. The subcutaneous fat thickness (SFT) showed an average of 10.50, 5.97 and 5.51mm for heifers, steers and intact males. In conclusion, the use of corn as a source of starch in the initial phase of the feed for young cattle is recommended. However, in the final stage, there is no distinction between sources of starch in the concentrate, which can be corn or sorghum. Females had higher EGS, while the males, castrated and intact, had higher feed efficiency in the final phase of confinement.

A fitorremediação é um processo promissor de descontaminação de solos em que a planta é utilizada como um mecanismo de alocação do agente contaminante e indesejável ao sistema tratado. Plantas de Cyperus rotundus, colhidas em área onde ocorreram descartes de resíduos industriais, foram avaliadas utilizando microscopia óptica (MO) e plasma de argônio indutivamente acoplado (ICP-AES). O trabalho objetivou avaliar as alterações causadas pelos poluentes químicos na estrutura morfológica do corpo epígeo de plantas juvenis de C. rotundus e seu potencial fitorremediador em comparação a plantas colhidas em região não poluída do mesmo solo (testemunha). As alterações anatômicas estruturais identificadas demonstram o potencial efeito poluidor dos contaminantes e também sugerem o comportamento hiperacumulador da planta avaliada.

Phytoremediation is a promising process of soil decontamination when the plant is used as a mechanism of allocation of the undesirable contaminant agent in the treated system. Samples of Cyperus rotundus collected in a contaminated area with industrial residues were evaluated through optical microscopy and inductively coupled plasma atomic emission spectroscopy (ICP - AES). This work aimed to evaluate the phytoremediator potential and the morphological alterations in young epigeous body of C. rotundus caused by pollutant chemicals in comparison with plants collected in the same soil with no contamination (witness). The observed anatomic alterations compared with the witnesses demonstrated the potential polluting effect of contaminants and also indicated the hyperaccumulating behavior of the analyzed plants.

Our aim was to determine the frequencies of the angiotensin-converting enzyme (ACE) gene alleles D and I and any associations to cardiovascular risk factors in a population sample from Rio de Janeiro, Brazil. Eighty-four adults were selected consecutively during a 6-month period from a cohort subgroup of a previous large cross-sectional survey in Rio de Janeiro. Anthropometric data and blood pressure measurements, echocardiogram, albuminuria, glycemia, lipid profile, and ACE genotype and serum enzyme activity were determined. The frequency of the ACE*D and I alleles in the population under study, determined by PCR, was 0.59 and 0.41, respectively, and the frequencies of the DD, DI, and II genotypes were 0.33, 0.51, and 0.16, respectively. No association between hypertension and genotype was detected using the Kruskal-Wallis method. Mean plasma ACE activity (U/mL) in the DD (N = 28), DI (N = 45) and II (N = 13) groups was 43 (in males) and 52 (in females), 37 and 39, and 22 and 27, respectively; mean microalbuminuria (mg/dL) was 1.41 and 1.6, 0.85 and 0.9, and 0.6 and 0.63, respectively; mean HDL cholesterol (mg/dL) was 40 and 43, 37 and 45, and 41 and 49, respectively, and mean glucose (mg/dL) was 93 and 108, 107 and 98, and 85 and 124, respectively. A high level of ACE activity and albuminuria, and a low level of HDL cholesterol and glucose, were found to be associated with the DD genotype. Finally, the II genotype was found to be associated with variables related to glucose intolerance.

Um sistema polivalente de análises químicas por injeção em fluxo foi proposto para a determinação de cálcio, magnésio e fósforo em digeridos ácidos de plantas. O-cresolphtaleina complexona (CPC) foi selecionado como reagente colorimétrico para a determinação de cálcio e magnésio e molibdato de amônio/vanadato de amônio para a determinação de fósforo. O sistema empregou tampão amônio-amônia (pH 10,5) e EGTA ou 8-hidroxiquinolina como agentes mascarantes para cálcio ou magnésio, respectivamente. Equações de calibração lineares foram obtidas, sendo que para facilitar a utilização do sistema proposto, foi utilizada curva analítica mista (até 40,0 mg Ca L-1, 10,0 mg Mg L-1 e 16,0 mg P L-1), não sendo observadas interferências entre os elementos. Os limites de detecção foram: 0,10; 0,05 e 0,004 <FONT FACE="Symbol">m</font>g L-1 respectivamente para Ca, Mg e P. Pequenas modificações no coeficiente das equações de calibração (normalmente < 3%) foram observadas após períodos de 4 h de trabalho. Os resultados apresentaram-se precisos (d.p.r. < 0,02), e em concordância com método alternativo (espectrometria de absorção atômica). Quando o sistema foi aplicado a análises em rotina, boa repetibilidade foi observada, sendo obtidas velocidade de amostragem de 107 amostras h-1 para magnésio e 75 amostras h-1 para cálcio e fósforo, enfatizando a simplicidade, robustez e versatilidade, inerentes aos sistemas de análises por injeção em fluxo.

A polyvalent flow injection system was proposed for spectrophotometric determination of calcium, magnesium and phosphorus in acid plant digests. O-cresolphtalein complexone (CPC) was the chromogenic reagent for calcium and magnesium determination, and ammonium molybdate/vanadate for phosphorus determination. Ammonium-ammonia was elected as buffer system, and EGTA or 8-hydroxyquinoline as the masking agents for calcium or magnesium. Linearity of the calibration equations was observed up to 40.0 mg Ca L-1, 10.0 mg Mg L-1 and 16.0 mg P L-1. Slight variations in the coefficients of the calibration equations (usually < 3 %) were found after 4-h working periods. Precise results (r.s.d. < 0.02), in agreement with alternative method (flame atomic absorption spectrometry) were obtained. When the system was applied to large scale analysis, good repeatability was observed, and a sample throughput, of about 107 h-1 for magnesium and 75 h-1 for calcium and phosphorus was achieved, emphasizing the simplicity, robustness and versatility inherent to flow analysis.

O objetivo do presente trabalho foi desenvolver uma metodologia adequada para análise intrablocos de experimentos em parcelas subdivididas quando os tratamentos primários estão dispostos em uma estrutura de delineamento em blocos incompletos parcialmente balanceados. Usando o método dos mínimos quadrados foram determinadas as expressões para as várias somas de quadrados que compõem a análise de variância e a composição do teste F.

The objective of this study was to develop a suitable methodology for the intrablock analysis of experiments in split-plot involving a two-way treatment structure when the design structure for whole plot experimental units is a partially balanced incomplete block design. Using the least square procedure expressions were developed to determine the sums of squares of the variance analysis and the justification of the F test.

O objetivo deste trabalho foi realizar um estudo para obtenção de uma solução adequada das equações normais de experimentos em parcelas subdivididas com tratamentos primários dispostos segundo uma estrutura de blocos incompletos parcialmente balanceados.

The objective of this study was to develop a suitable methodology for the solution of normal equations in split-plot experiments involving two-way treatment structures when the design for the whole plot experimental units is a partially balanced incomplete block design.

A Síndrome de Tolosa-Hunt (STH) ou oftalmoplegia dolorosa é associada a granulomatose inespecifica de etiologia desconhecida que acomete a fissura orbitária superior. Compromete estruturas nervosas e vasculares causando quadro clínico variável que sempre se associa a dor e apresenta resposta favorável à corticoterapia. Processos inflamatórios, tumores e aneurismas dessa região podem causar sintomas semelhantes. A tomografia computadorizada, a angiografia cerebral e a flebografia orbitária são métodos de imagem indicados para orientar o diagnóstico. Revisamos os resultados destes exames radiológicos de oito pacientes atendidos no Hospital São Paulo no período 1989 a 1991, com diagnóstico de STH segundo os critérios de Hunt e Hannerz. A análise das alterações da flebografia orbitária, de acordo com a sistematização feita por Hannerz e col. mostrou ser este exame inespecífico, porém capaz de orientar melhor o diagnóstico.

Tolosa-Hunt syndrome (THS), or painful ophthalmoplegia is associated to a non-specific granulomatosis of unknown etiology, that involves the superior orbital fissure and its nervous and vascular structures. The clinical picture that responds to steroid therapy, is variable and is always associated with pain. Inflammatory conditions, tumors and aneurysms can produce similar symptoms. Computed tomography, cerebral angiography and orbital phlebography are the imaging methods of choice for making the diagnosis. We revised the results of these radiological examinations of eight patients seen at the Hospital São Paulo from 1989 to 1991, with the diagnosis of THS according to Hunt and Hannerz criteria. The analysis of orbital phlebography changes based upon Hannerz et al. systematization showed non-specific features, but those were able to help the diagnosis.