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1.
Long-term oncological and surgical outcomes after Video Endoscopic Inguinal Lymphadenectomy (VEIL) in patients with penile cancer
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Tobias-Machado, Marcos
; Ornellas, Antonio A.
; Hidaka, Alexandre K.
; Medina, Luis G.
; Mattos, Pablo A. L.
; Besio, Ruben S.
; Abreu, Diego
; Castro, Pedro R.
; Nishimoto, Ricardo H.
; Astigueta, Juan
; Dourado, Aurus
; Machado, Roberto D.
; Magnabosco, Wesley J.
; Corona-Montes, Victor
; Villoldo, Gustavo M.
; Zampolli, Hamilton C.
; Taha, Anis
; Auad, Pericles R.
; Faria, Eliney F.
; Arantes, Paulo B. O.
; Tavares, Alessandro
; Nascimento, Francisco S. M. S.
; Brazão Jr., Eder S.
; Rocha, Maurício M.
; Costa, Walter H.
; Panico, Vinicius
; Reis, Leonardo O.
; Almeida-Carrera, Roberto J.
; Silva, Rafael C.
; Zequi, Stênio C.
; Calixto, José R. R.
; Sotelo, Rene
.
ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer.
2.
Characterization of Flavonoids Profiles in Polar Extracts from Croton grewioides Baill. Using Ultra-High Resolution Mass Spectrometry Baill UltraHigh Ultra High
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Nascimento, Pedro E. S. do
; Prado, Vilma M. J.
; Jesus, Raphael A. de
; Silva, Wenes R. da
; Wisniewski Jr., Alberto
; Sá Filho, José C. F.
; Blank, Arie F.
; Souza, Daniel A. de
; Wartha, Elma R. S. A.
; Nogueira, Paulo C. L.
; Moraes, Valéria R. S.
.
Research to identify plant bioactive compounds led to the evolution of extraction methods. This study optimized ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) to extract the flavonoids of Croton grewioides Baill. species contributing to characterize the polar extract profile and understand the antioxidant potential of these plant constituents. The use of experimental design and statistical treatments enabled the determination of the ideal conditions for each technique. The extracts of five accessions of C. grewioides were obtained under the optimized extraction conditions and were analyzed using ultra high-resolution mass spectrometry (FT-Orbitrap MS) operating at negative ionization mode for flavonoids detection. The accurate experimental mass obtained to the main compounds was used to attribute the molecular formula. Chemical structures of the main compounds detected were proposed using structure data bases. Chemometric analysis were performed with two FT-Orbitrap MS spectra samples using the identified metabolites and, the antioxidant activity data, showing that for this species the MAE was most effective in extracting the antioxidant compounds. It was possible to propose the structures for forty compounds in the C. grewioides extracts, demonstrating the excellent performance of the FT-Orbitrap MS in providing information on the chemical profile of polar compounds in plant extracts. methods ultrasoundassisted ultrasound assisted UAE (UAE microwaveassisted microwave (MAE Baill constituents technique C highresolution high resolution FTOrbitrap FT Orbitrap detection formula bases
3.
Occurrence of clinical laminitis after adaptation to confinement: effects on morphology, density, and mineral composition of the hoof of Nellore cattle after finishing
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Freitas, Sabrina L.R.
; Queiroz, Paulo José B.
; Fernandes, Juliano José R.
; Nascente, Eduardo P.
; Santos, Adriana S.
; Nascimento, Kaique S.
; Silva, Luiz Antônio F.
.
ABSTRACT: Laminitis is a disease that affects the dermis and epidermis of the bovine hoof, generating changes in the hoof capsule. This study evaluated the effects of clinical laminitis diagnosed after the adaptation phase to confinement on the morphology, density, and mineral composition of the hoof of Nellore cattle after finishing. The animals were separated in the first weeks of confinement into a sick group (SG), with clinical laminitis, and a healthy group (HG). SG animals had higher heel length, dorsal wall length, toe height, and diagonal hoof length (p<0.05) than healthy animals. The dermal laminae had similar measurements for thickness, length, and spacing between them between SG and HG. Animals with laminitis showed congestion, hemorrhage, and basement membrane irregularities on histology. Computed microtomography (μCT) revealed that the hoof density of sick animals is lower than healthy ones. According to the mineral composition by energy-dispersive X-ray fluorescence (ED-XRF) spectrometry, the hooves of animals with laminitis (SG) and healthy ones (HG) were not biochemically different. Therefore, the occurrence of clinical laminitis in Nellore cattle in the first weeks of confinement causes an increase in the morphometric parameters of the hoof capsule and a reduction in the density of the abaxial hoof wall evaluated after the finishing period. This disease does not promote changes in the histomorphometric parameters of the dermal laminae and the percentage of minerals in the abaxial hoof wall.
RESUMO: A laminite é uma doença que afeta a derme e epiderme do casco de bovinos gerando alterações no estojo córneo. O estudo avaliou os efeitos da laminite clínica diagnosticada após a fase de adaptação ao confinamento na morfologia, densidade e composição mineral do casco de bovinos da raça Nelore após terminação. Nas primeiras semanas de confinamento, os animais foram separados em um grupo doente (GD) com laminite clínica e em um grupo saudável (GS). Os animais do GD apresentaram maior comprimento de talão, comprimento da parede dorsal, altura da pinça e comprimento diagonal do casco (p<0,05) do que os saudáveis. As lâminas dérmicas tiveram medidas semelhantes para espessura, comprimento e espaçamento entre elas entre GD e GS. Animais doentes apresentaram congestão, hemorragia e irregularidades da membrana basal na histologia. A microtomografia computadorizada (μCT) revelou que a densidade do casco de animais doentes é menor do que o saudável. Para a composição mineral por meio da espectrometria de fluorescência de raio-X por dispersão de energia (ED-XRF), o casco dos animais doentes (GD) e dos saudáveis (GS), não se mostraram diferentes bioquimicamente. Conclui-se que a ocorrência de laminite clínica em bovinos da raça Nelore nas primeiras semanas de confinamento ocasiona aumento de parâmetros morfométricos do estojo córneo e redução da densidade da parede abaxial do casco, avaliados após o período de terminação. Essa enfermidade não promove modificações nos parâmetros histomorfométricos das lâminas dérmicas e na porcentagem de minerais da parede abaxial do casco.
4.
COVID-19 in the Perioperative Period of Cardiovascular Surgery: the Brazilian Experience
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Gomes, Walter J.
; Rocco, Isadora
; Pimentel, Wallace S.
; Pinheiro, Aislan H. B.
; Souza, Paulo M. S.
; Costa, Luiz A. A.
; Teixeira, Marjory M. P.
; Ohashi, Leonardo P.
; Bublitz, Caroline
; Begot, Isis
; Moreira, Rita Simone L
; Hossne Jr, Nelson A.
; Vargas, Guilherme F.
; Branco, João Nelson R.
; Teles, Carlos A.
; Medeiros, Eduardo A. S.
; Sáfadi, Camila
; Rampinelli, Amândio
; Moratelli Neto, Leopoldo
; Rosado, Anderson Rosa
; Mesacasa, Franciele Kuhn
; Capriata, Ismael Escobar
; Segalote, Rodrigo Coelho
; Palmieri, Deborah Louize da Rocha Vianna
; Jardim, Amanda Cristina Mendes
; Vianna, Diego Sarty
; Coutinho, Joaquim Henrique de Souza Aguiar
; Jazbik, João Carlos
; Coutinho, Henrique Madureira da Rocha
; Kikuta, Gustavo
; Almeida, Zely Sant'Anna Marotti de
; Feguri, Gibran Roder
; Lima, Paulo Ruiz Lucio de
; Franco, Anna Carolina
; Borges, Danilo de Cerqueira
; Cruz, Felipe Ramos Honorato De La
; Croti, Ulisses Alexandre
; Borim, Bruna Cury
; Marchi, Carlos Henrique De
; Goraieb, Lilian
; Postigo, Karolyne Barroca Sanches
; Jucá, Fabiano Gonçalves
; Oliveira, Fátima Rosane de Almeida
; Souza, Rafael Bezerra de
; Zilli, Alexandre Cabral
; Mas, Raul Gaston Sanchez
; Bettiati Junior, Luiz Carlos
; Tranchesi, Ricardo
; Bertini Jr, Ayrton
; Franco, Leandro Vieira
; Fernandes, Priscila
; Oliveira, Fabiana
; Moraes Jr, Roberto
; Araújo, Thiago Cavalcanti Vila Nova de
; Braga, Otávio Penna
; Pedrosa Sobrinho, Antônio Cavalcanti
; Teixeira, Roberta Tavares Barreto
; Camboim, Irla Lavor Lucena
; Gomes, Eduardo Nascimento
; Reis, Pedro Horigushi
; Garcia, Luara Piovan
; Scorsioni, Nelson Henrique Goes
; Lago, Roberto
; Guizilini, Solange
.
Brazilian Journal of Cardiovascular Surgery
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Abstract Introduction: We investigated the clinical course and outcomes of patients submitted to cardiovascular surgery in Brazil and who had developed symptoms/signs of coronavirus disease 2019 (COVID-19) in the perioperative period. Methods: A retrospective multicenter study including 104 patients who were allocated in three groups according to time of positive real time reverse transcriptase-polymerase chain reaction (RT-PCR) for the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2): group 1, patients who underwent cardiac surgery > 10 days after positive RT-PCR; group 2, patients with a positive RT-PCR within 10 days before or after surgery; group 3, patients who presented positive RT-PCR > 10 days after surgery. The primary outcome was mortality and secondary outcomes were postoperative complications, intensive care unit (ICU) length of stay, and postoperative days of hospitalization. Results: The three groups were similar with respect to age, the European System of Cardiac Operative Risk Evaluation score, and comorbidities, except hypertension. Postoperative complications and death were significantly higher in groups 2 and 3 than in group 1, and no significant difference between groups 2 and 3 was seen. Group 2 showed a high prevalence of surgery performed as an urgent procedure. Although no significant differences were observed in ICU length of stay, total postoperative hospitalization time was significantly higher in group 3 than in groups 1 and 2. Conclusion: COVID-19 affecting the postoperative period of patients who underwent cardiovascular surgery is associated with a higher rate of morbidity and mortality. Delaying procedures in RT-PCR-positive patients may help reduce risks of perioperative complications and death.
5.
Long-term obesity is associated with depression and neuroinflammation
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Lorena, Fernanda B.
; Nascimento, Bruna P. P do
; Camargo, Esther L. R. A.
; Bernardi, Maria M.
; Fukushima, André R.
; Panizza, Julia do N.
; Nogueira, Paula de B.
; Brandão, Marllos E. S.
; Ribeiro, Miriam O.
.
ABSTRACT Objective: Obesity is characterized by a state of chronic, low-intensity systemic inflammation frequently associated with insulin resistance and dyslipidemia. Materials and methods: Given that chronic inflammation has been implicated in the pathogenesis of mood disorders, we investigated if chronic obesity that was initiated early in life – lasting through adulthood – could be more harmful to memory impairment and mood fluctuations such as depression. Results: Here we show that pre-pubertal male rats (30 days old) treated with a high-fat diet (40%) for 8-months gained ~50% more weight when compared to controls, exhibited depression and anxiety-like behaviors but no memory impairment. The prefrontal cortex of the obese rats exhibited an increase in the expression of genes related to inflammatory response, such as NFKb, MMP9, CCl2, PPARb, and PPARg. There were no alterations in genes known to be related to depression. Conclusion: Long-lasting obesity with onset in prepuberal age led to depression and neuroinflammation but not to memory impairment.
https://doi.org/10.20945/2359-3997000000400
36 downloads
6.
Evaluation of the Synthesis of Zeolite SSZ-13 using Choline Chloride and Different Crystallization Seeds and its Application in the Thermocatalytic Degradation of Ultra-High Molecular Weight Polyethylene
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Solano, Julyane R. S.
; Nascimento, Raul C. S.
; Silva, David C. M.
; Silva, Diogo P. S.
; Sousa, Lenivaldo V.
; Silva, Bruno J. B.
; Alencar, Soraya L.
; Urbina, Maritza M.
; Quintela, Paulo H. L.
; Silva, Antonio O. S.
.
Abstract The synthesis of SSZ-13 was studied by evaluating the influence of two types of crystallization seeds with CHA structure (S1 seeds prepared in fluoride medium and S2 seeds by the conversion of zeolite Y), SiO2/Al2O3 (28, 40, 60 and 100) and OH/SiO2 (0.6 and 0.8) ratios. Choline chloride was used as the structure-directing agent and the crystallizations occurred at 140 ºC from 24 to 96 h. S1 seeds led to the coexistence of zeolites SSZ-13 and P, while S2 seeds promoted in all SiO2/Al2O3 ratios and OH/SiO2 = 0.8, the formation of pure SSZ-13 after 24 h. The SSZ-13 samples are formed by clusters of crystals with cuboid-like morphology and exhibited textural properties typical of CHA framework. SSZ-13 zeolite led to a decrease in the temperature and the activation energy required for the degradation of ultra-high molecular weight polyethylene polymer, demonstrating its efficiency as catalyst in this process.
https://doi.org/10.1590/1980-5373-mr-2021-0066
152 downloads
7.
Mango (Mangifera indica) Aroma Discriminate Cultivars and Ripeness Stages
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Mesquita, Paulo R. R.
; Pena, Laiane C.
; Santos, Fábio N. dos
; Oliveira, Camilo C. de
; Magalhães-Junior, Jairo T.
; Nascimento, Antonio S.
; Rodrigues, Frederico M.
.
Journal of the Brazilian Chemical Society
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Mango (Mangifera indica) has many nutritional attributes that stimulate its consumption, such as sweetness, balanced acidity and intense fruity aroma. In this study, it was demonstrated that both the variety and ripening stage have a significant impact on the aroma of mango. The volatile compound profile of “Tommy Atkins”, “palmer”, “espada” and “Carlota” varieties in green and ripe stages were characterized by headspace solid-phase microextraction gas chromatography mass spectrometry (HS-SPME/GC-MS). Thirty-three volatile compounds were identified, 30 from “Tommy Atkins”, 29 from “espada”, 25 from “palmer” and 10 from the “Carlota” variety. The major compounds were monoterpene and sesquiterpene hydrocarbons, regardless of the degree of maturity. However, the oxygenated monoterpenes, esters and lactones were also identified as minor compounds. δ-3-Carene terpene is the most abundant in the varieties “Tommy Atkins” and “palmer”, α-terpinolene is the most abundant in the cv. “espada”, and myrcene in cv. “Carlota”. The profile’s aroma obtained by HS-SPME/GC-MS associated with multivariate data analysis lead to discrimination of four mango varieties in green and ripeness stages. Therefore, the volatile compounds markers of fruit maturation identified in this study can be used to increase the efficiency of mango sorting to improve quality control and meet the consumer market requirements.
https://doi.org/10.21577/0103-5053.20200027
417 downloads
8.
Photo-selective nets and potassium concentrations on Costus lasius crop
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Sampaio, Pedro R. F.
; Rodrigues, Paulo H. V.
; Duarte, Sergio N.
; Sousa Neto, Osvaldo N. de
; Nascimento, Jéssica G.
; Piedade, Sônia M. de S.
.
Revista Brasileira de Engenharia Agrícola e Ambiental
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RESUMO A prática da utilização de malhas de diferentes colorações, associada à técnica de fertirrigação, representa um novo conceito agrotecnológico que visa promover respostas fisiológicas específicas ao crescimento e à produção que são reguladas pela luz. Neste sentido, o objetivo da pesquisa foi avaliar o efeito, em cultivo protegido, de malhas fotoconversoras e de concentrações de potássio na solução de fertirrigação sobre o desempenho horticultural da Costus lasius. A pesquisa foi conduzida na Universidade de São Paulo, ESALQ, Piracicaba, SP. Foram realizados três experimentos separadamente, para avaliar cinco concentrações de potássio na solução de fertirrigação (40, 80, 120, 160 e 200 mg L-1) em três colorações de malhas fotoconversoras (vermelha, azul e preta), no delineamento em blocos casualizados com cinco repetições. Foram avaliadas as variáveis altura de planta, número de folhas, caules e botões de flores. Os dados foram submetidos à análise conjunta de experimentos. A associação entre a concentração de 200 mg L-1 de K+ e a malha fotoconversora de cor preta resultou em maior produção de hastes e inflorescências.
ABSTRACT The practice of using different photo-selective nets, associated with the technique of fertigation, represents a new agrotechnological concept that aims to promote specific physiological responses for growth and production, which are regulated by light. The aims of the study were to evaluate the effect of photo-selective nets and potassium concentrations in the fertigation solution on the horticultural performance of Costus lasius. The study was conducted at the Universidade de São Paulo, ESALQ, Piracicaba, SP, Brazil. Three experiments were carried out separately to evaluate five potassium concentrations in the fertigation solution (40, 80, 120, 160 and 200 mg L-1) under three colours of photo-selective nets (red, blue and black) in a randomized block design, with five repetitions. The variables plant height and numbers of leaves, stems and flower buds were evaluated. The data were submitted to the joint analysis of experiments. The association between the K+ concentration of 200 mg L-1 and the black photo-selective net results in higher stem and inflorescence production.
https://doi.org/10.1590/1807-1929/agriambi.v24n3p143-148
527 downloads
9.
Fighting HIV/AIDS in a developing country: lessons from a small cohort from the largest Brazilian city
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Casseb, Jorge
; Veiga, Ana Paula R.
; Magri, Marcello M. C.
; Monteiro, Mariana A.
; Rocha, Rosana C.
; Gascon, Maria Rita P.
; Domingues-Ferreira, Mauricio
; Polis, Thales J. B.
; Nascimento, Najara A. de Lima
; Limongelli, Isadora
; Oliveira, Ícaro S.
; Prandi, Gabriela Caetano
; Costa, Livia M. C. B. Villares
; Fonseca, Luiz A. M.
; Duarte, Alberto J. S.
.
Revista do Instituto de Medicina Tropical de São Paulo
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https://doi.org/10.1590/s1678-9946202062058
221 downloads
10.
Hydration status, sweating rate, heart rate and perceived exertion after running sessions in different relative humidity conditions: a randomized controlled trial
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Nascimento, Matheus A. do
; Guilherme, Flávio Ricardo
; Ferreira, Sandra A.
; Januário, Renata S.B.
; Ribeiro, Alex S.
; Mayhew, Jerry L.
; Voltarelli, Fabrício
; Cyrino, Letícia T.
; Sugihara Junior, Paulo
; Silva, Danilo Rodrigues Pereira da
; Queiroga, Marcos R.
.
RESUMO Analisar o impacto da corrida em diferentes humidade relativa (HR) (normal (53%), muito alto (94%)), estado de hidratação (EH), taxa de transpiração (TT), freqüência cardíaca (FC), esforço percebido (RPE). Quatorze homens (25,2 anos) realizaram duas sessões de corrida em esteira. Massa corporal (HR normal: -1,35%, HR muito alta: -1,65%) e EH reduziram (HR normal: -14,8%, HR muito alta: -20,8%), RPE (HR normal: + 26%, HR muito alta: + 28%), aumento da FC (HR muito alta: + 5.8%: 20-40 min, + 3.1%: 40-60 min, HR normal: + 3%: 20-40 min; + 2.3%: 40-60 min). TT foi significantemente diferente entre HR (HR normal: -14.8%, HR muito alta: -20.8%). Há um impacto negativo na corrida em EH, massa corporal, FC e RPE em homens com HR normal e muito alta, e TT é mais afetada com HR muito alta.
ABSTRACT The purpose of this study was to analyze the impact of running in normal relative humidity (RH = 53%) and very high RH (94%) on hydration status (HS), sweating rate (SR), heart rate (HR), and rating of perceived exertion (RPE). Fourteen men (25.2 ± 6.6 yrs) performed two sessions of treadmill running. Body mass (normal RH: -1.35%, very high RH: -1.65%) HS (normal RH: -14.8%; very high RH: -20.8%) were reduced while RPE (normal RH: +26%; very high RH: +28%) and HR were increased (very high RH: +5.8%: 20-40 min, +3.1%: 40-60 min, +3%: 20-40 min; +2.3%: 40-60 min for normal RH). As expected, SR was significantly greater during very high RH (-20/8%) compared to normal RH (-14.8%). Running in very high RH at moderate temperature has a greater negative impact on SR, HS, body mass, HR, RPE in men than running in normal RH at the same temperature, which places the runner in greater danger of heat illnesses such as heat exhaustion or heat stroke.
RESUMEN El objetivo de este estudio fue analizar las repercusiones de correr con diferentes niveles de humedad relativa (HR) (normal [53%], muy alta [94%]) sobre el estado de hidratación (EH), la tasa de sudoración (TS), la frecuencia cardíaca (FC) y el esfuerzo percibido (EP). Catorce hombres (25,2 ± 6,6 años) realizaron dos sesiones en cinta de correr. La masa corporal (HR normal: -1,35%; HR muy alta: -1,65%) y el EH (HR normal: -14,8%; HR muy alta: -20,8%) se redujeron, mientras que el EP (HR normal: +26%; HR muy alta: +28%) y la FC (HR muy alta: +5,8%: 20-40 min; +3,1%: 40-60 min; HR normal: + 3%: 20-40 min; + 2,3%: 40-60) aumentaron. La TS fue considerablemente diferente entre las HR (HR normal: -14,8%; muy alta HR: -20,8%). Hay repercusiones negativas por correr con una HR muy alta y una temperatura moderada en TS, EH, masa corporal, FC y EP en corredores que corren a una HR normal a la misma temperatura, lo que sitúa a estos en gran peligro de sufrir un enfermedad provocada por el calor, como agotamiento por calor o golpe de calor.
https://doi.org/10.1016/j.rbce.2018.10.003
635 downloads
11.
Brazilian legislation on genetic heritage harms Biodiversity Convention goals and threatens basic biology research and education
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ALVES, RUY JOSÉ V.
; WEKSLER, MARCELO
; OLIVEIRA, JOÃO A.
; BUCKUP, PAULO A.
; POMBAL JR., JOSÉ P.
; SANTANA, HÉLCIO R.G.
; PERACCHI, ADRIANO LÚCIO
; KELLNER, ALEXANDER W.A.
; ALEIXO, ALEXANDRE
; LANGGUTH, ALFREDO
; ALMEIDA, ALZIRA MARIA P. DE
; ALBERNAZ, ANA LUISA
; RIBAS, CAMILA C.
; ZILBERBERG, CARLA
; GRELLE, CARLOS EDUARDO V.
; ROCHA, CARLOS F.D.
; LAMAS, CARLOS JOSÉ E.
; HADDAD, CÉLIO F.B.
; BONVICINO, CIBELE R.
; PRADO, CYNTHIA P.A.
; LIMA, DANIELA O. DE
; ROSSA-FERES, DENISE C.
; SANTOS, FABRÍCIO R. DOS
; SALIMENA, FÁTIMA REGINA G.
; PERINI, FERNANDO A.
; BOCKMANN, FLÁVIO A.
; FRANCO, FRANCISCO LUÍS
; GIUDICE, GISELE M.L. DEL
; COLLI, GUARINO R.
; VIEIRA, IMA CÉLIA G.
; MARINHO-FILHO, JADER
; WERNECK, JANE M.C.F.
; SANTOS, JORGE A.D. DOS
; NASCIMENTO, JORGE LUIZ DO
; NESSIMIAN, JORGE LUIZ
; CORDEIRO, JOSÉ LUIS P.
; CLARO, KLEBER DEL
; SALLES, LEANDRO O.
; CASATTI, LILIAN
; PY-DANIEL, LUCIA HELENA R.
; SILVEIRA, LUÍS FÁBIO
; TOLEDO, LUÍS FELIPE
; OLIVEIRA, LUIZ F. DE
; MALABARBA, LUIZ ROBERTO
; SILVA, MARCELO D. DA
; COURI, MÁRCIA S.
; MARTINS, MÁRCIO
; TAVARES, MARCOS D.S.
; SOBRAL, MARCOS EDUARDO G.
; VIEIRA, MARCUS VINÍCIUS
; OLIVEIRA, MARIA DE LOURDES A.
; PINNA, MÁRIO DE
; HOPKINS, MICHAEL J.G.
; SOLÉ, MIRCO
; MENEZES, NAÉRCIO A.
; PASSOS, PAULO
; D’ANDREA, PAULO SERGIO
; PINTO, PEDRO C.E.A.
; VIANA, PEDRO L.
; TOLEDO, PETER M.
; REIS, ROBERTO E.
; VILELA, ROBERTO
; BASTOS, ROGÉRIO P.
; COLLEVATTI, ROSANE G.
; CERQUEIRA, RUI
; CASTROVIEJO-FISHER, SANTIAGO
; CARAMASCHI, ULISSES
.
https://doi.org/10.1590/0001-3765201820180460
7311 downloads
12.
Transmission, serologic and tissue responses in chickens vaccinated with Mycoplasma gallisepticum F strain (MG-F)
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Machado, Leandro S.
; Santos, Felipe F. dos
; Santos, Lídia M.M. dos
; Tortelly, Rogério
; Pimentel, Jorge C.
; Sesti, Luiz
; Pereira, Virgínia L.A.
; Nascimento, Elmiro R.
.
Resumo: Mycoplasma gallisepticum cepa F (MG-F) é altamente utilizada em vacinação de poedeiras. MG-F confere bons níveis de proteção às galinhas, deslocando MG de campo ou diminuindo o número deles no trato respiratório. Soroaglutinação Rápida (SAR), ELISA e PCR são testes no monitoramento da micoplasmose, enquanto a histopatologia, mesmo não sendo rotineira, é usada para avaliar a resposta das aves à infecção por MG. O objetivo deste estudo foi avaliar a transmissibilidade, soroconversão e alterações teciduais de MG-F em galinhas. Um total de 100 galinhas SPF foi utilizado, sendo 40 delas não vacinadas (G1), 40 vacinadas na 8ª semana de idade com MG-F (Ceva Saúde Animal, São Paulo/SP, Brasil) (G2) e 20 imunizadas por contato com aves do G2 (G3). Soros e suabes traqueais foram obtidos na 8ª, 12ª, 15ª, 18ª, 20ª, 24ª semana para monitoramento por SAR, ELISA e PCR. Fragmentos de traqueia e saco aéreo, para microscopia, foram feitas após necropsias na 15ª e 24ª semana. Até a 12ª semana não houve diferença significativa entre os grupos pela SAR. Houve reação a SAR a partir da 15ª semana com as seguintes médias: G1 (1,7; 1,76; 0,1; 0,15), G2 (7,81; 7,65; 8,25; 6,29) e G3 (8,1; 8,5; 9,5; 6,16), enquanto por ELISA a soroconversão ocorreu a partir da 18ª semana com médias de densidades óticas de G1 (0,19; 0,14; 0,16), G2 (0,47; 0,45; 0,41) e G3 (0,55; 0,51; 0,51). Todas as aves do G3 apresentaram positividade pela PCR sete semanas após exposição. Não houve diferença significativa entre as medias dos escores de saco aéreo entre os grupos, na 15ª semana (0,20; 0,55; 0,32) e 24ª semana (0,15; 0,80 e 0,66). Em relação à traqueia, G2 apresentou média maior na 15ª semana (0,48) que G3 (0,00) e G1 (0,10). Alterações em G3 foram observadas somente na 24ª semana onde as médias foram de 0,08(G1); 0,46 (G2) e 1,00 (G3), havendo significância (p<0,05) entre G1 e G3. SAR e PCR foram capazes de detectar a transmissão de MG-F de forma precoce em relação ao ELISA. Em relação ao G1 (controle negativo) as reações teciduais para os grupos vacinados foram mais intensas na 24ª semana, o que tudo indica sendo resposta à vacinação.
Abstract: MG-F protects chickens from MG Mycoplasmosis and monitoring is done by serology (SAR and ELISA) and PCR. Histopathology is used to evaluate bird response to MG. This study evaluated MG-F profile vaccination in SPF chicken. This trial used 100 chickens, being 40 unvaccinated (G1), 40 eye-drop vaccinated at 8 weeks of age with MG-F ( Ceva Animal Health , São Paulo , SP , Brazil ) (G2) and 20 immunized by contact (G3) . Samples were obtained on the 8th, 12th, 15th, 18th, 20th and 24th week for SAR, ELISA and PCR. Fragments of trachea and air sac, for microscopy, were got after necropsies on the 15th and 24th week. Up to 12 weeks there was no significant difference among groups by SAR. SAR reactions appeared from the 15th week with these averages: G1 (1.7, 1.76 , 0.1, 0.15) , G2 (7.81, 7.65, 8.25, 6.29) and G3 (8.1, 8.5, 9.5, 6.16), while by ELISA it occurred after the 18th week with optical densities averages: G1 (0.19, 0.14, 0.16) , G2 (0.47, 0.45, 0.41) and G3 (0.55, 0.51, 0.51) . Positivity in G3 by PCR occurred seven weeks after exposure. At the 15th week the air sac score means were 0.20, 0.55, and 0.32 and 24th week were 0.15, 0.80 and 0.66 (p>0.05). For trachea, G2 (0.48) yielded higher score average than G1 (0.10) and G3 (0.00) on the 15th week. Changes in G3 were seen only at 24th week, being this average (1.00) significantly different (p<0,05) from G1 (0.08) and G2 (0.46). SAR and PCR detected MG-F in G3 earlier than ELISA. Higher tracheal changes for G2 and G3 as compared to G1 could be ascribed to MG-F vaccine infection.
https://doi.org/10.1590/S0100-736X2016000500008
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13.
Growing knowledge: an overview of Seed Plant diversity in Brazil
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Zappi, Daniela C.
; Filardi, Fabiana L. Ranzato
; Leitman, Paula
; Souza, Vinícius C.
; Walter, Bruno M.T.
; Pirani, José R.
; Morim, Marli P.
; Queiroz, Luciano P.
; Cavalcanti, Taciana B.
; Mansano, Vidal F.
; Forzza, Rafaela C.
; Abreu, Maria C.
; Acevedo-Rodríguez, Pedro
; Agra, Maria F.
; Almeida Jr., Eduardo B.
; Almeida, Gracineide S.S.
; Almeida, Rafael F.
; Alves, Flávio M.
; Alves, Marccus
; Alves-Araujo, Anderson
; Amaral, Maria C.E.
; Amorim, André M.
; Amorim, Bruno
; Andrade, Ivanilza M.
; Andreata, Regina H.P.
; Andrino, Caroline O.
; Anunciação, Elisete A.
; Aona, Lidyanne Y.S.
; Aranguren, Yani
; Aranha Filho, João L.M.
; Araújo, Andrea O.
; Araújo, Ariclenes A.M.
; Araújo, Diogo
; Arbo, María M.
; Assis, Leandro
; Assis, Marta C.
; Assunção, Vivian A.
; Athiê-Souza, Sarah M.
; Azevedo, Cecilia O.
; Baitello, João B.
; Barberena, Felipe F.V.A.
; Barbosa, Maria R.V.
; Barros, Fábio
; Barros, Lucas A.V.
; Barros, Michel J.F.
; Baumgratz, José F.A.
; Bernacci, Luis C.
; Berry, Paul E.
; Bigio, Narcísio C.
; Biral, Leonardo
; Bittrich, Volker
; Borges, Rafael A.X.
; Bortoluzzi, Roseli L.C.
; Bove, Cláudia P.
; Bovini, Massimo G.
; Braga, João M.A.
; Braz, Denise M.
; Bringel Jr., João B.A.
; Bruniera, Carla P.
; Buturi, Camila V.
; Cabral, Elza
; Cabral, Fernanda N.
; Caddah, Mayara K.
; Caires, Claudenir S.
; Calazans, Luana S.B.
; Calió, Maria F.
; Camargo, Rodrigo A.
; Campbell, Lisa
; Canto-Dorow, Thais S.
; Carauta, Jorge P.P.
; Cardiel, José M.
; Cardoso, Domingos B.O.S.
; Cardoso, Leandro J.T.
; Carneiro, Camila R.
; Carneiro, Cláudia E.
; Carneiro-Torres, Daniela S.
; Carrijo, Tatiana T.
; Caruzo, Maria B.R.
; Carvalho, Maria L.S.
; Carvalho-Silva, Micheline
; Castello, Ana C.D.
; Cavalheiro, Larissa
; Cervi, Armando C.
; Chacon, Roberta G.
; Chautems, Alain
; Chiavegatto, Berenice
; Chukr, Nádia S.
; Coelho, Alexa A.O.P.
; Coelho, Marcus A.N.
; Coelho, Rubens L.G.
; Cordeiro, Inês
; Cordula, Elizabeth
; Cornejo, Xavier
; Côrtes, Ana L.A.
; Costa, Andrea F.
; Costa, Fabiane N.
; Costa, Jorge A.S.
; Costa, Leila C.
; Costa-e-Silva, Maria B.
; Costa-Lima, James L.
; Cota, Maria R.C.
; Couto, Ricardo S.
; Daly, Douglas C.
; De Stefano, Rodrigo D.
; De Toni, Karen
; Dematteis, Massimiliano
; Dettke, Greta A.
; Di Maio, Fernando R.
; Dórea, Marcos C.
; Duarte, Marília C.
; Dutilh, Julie H.A.
; Dutra, Valquíria F.
; Echternacht, Lívia
; Eggers, Lilian
; Esteves, Gerleni
; Ezcurra, Cecilia
; Falcão Junior, Marcus J.A.
; Feres, Fabíola
; Fernandes, José M.
; Ferreira, D.M.C.
; Ferreira, Fabrício M.
; Ferreira, Gabriel E.
; Ferreira, Priscila P.A.
; Ferreira, Silvana C.
; Ferrucci, Maria S.
; Fiaschi, Pedro
; Filgueiras, Tarciso S.
; Firens, Marcela
; Flores, Andreia S.
; Forero, Enrique
; Forster, Wellington
; Fortuna-Perez, Ana P.
; Fortunato, Reneé H.
; Fraga, Cléudio N.
; França, Flávio
; Francener, Augusto
; Freitas, Joelcio
; Freitas, Maria F.
; Fritsch, Peter W.
; Furtado, Samyra G.
; Gaglioti, André L.
; Garcia, Flávia C.P.
; Germano Filho, Pedro
; Giacomin, Leandro
; Gil, André S.B.
; Giulietti, Ana M.
; A.P.Godoy, Silvana
; Goldenberg, Renato
; Gomes da Costa, Géssica A.
; Gomes, Mário
; Gomes-Klein, Vera L.
; Gonçalves, Eduardo Gomes
; Graham, Shirley
; Groppo, Milton
; Guedes, Juliana S.
; Guimarães, Leonardo R.S.
; Guimarães, Paulo J.F.
; Guimarães, Elsie F.
; Gutierrez, Raul
; Harley, Raymond
; Hassemer, Gustavo
; Hattori, Eric K.O.
; Hefler, Sonia M.
; Heiden, Gustavo
; Henderson, Andrew
; Hensold, Nancy
; Hiepko, Paul
; Holanda, Ana S.S.
; Iganci, João R.V.
; Imig, Daniela C.
; Indriunas, Alexandre
; Jacques, Eliane L.
; Jardim, Jomar G.
; Kamer, Hiltje M.
; Kameyama, Cíntia
; Kinoshita, Luiza S.
; Kirizawa, Mizué
; Klitgaard, Bente B.
; Koch, Ingrid
; Koschnitzke, Cristiana
; Krauss, Nathália P.
; Kriebel, Ricardo
; Kuntz, Juliana
; Larocca, João
; Leal, Eduardo S.
; Lewis, Gwilym P.
; Lima, Carla T.
; Lima, Haroldo C.
; Lima, Itamar B.
; Lima, Laíce F.G.
; Lima, Laura C.P.
; Lima, Leticia R.
; Lima, Luís F.P.
; Lima, Rita B.
; Lírio, Elton J.
; Liro, Renata M.
; Lleras, Eduardo
; Lobão, Adriana
; Loeuille, Benoit
; Lohmann, Lúcia G.
; Loiola, Maria I.B.
; Lombardi, Julio A.
; Longhi-Wagner, Hilda M.
; Lopes, Rosana C.
; Lorencini, Tiago S.
; Louzada, Rafael B.
; Lovo, Juliana
; Lozano, Eduardo D.
; Lucas, Eve
; Ludtke, Raquel
; Luz, Christian L.
; Maas, Paul
; Machado, Anderson F.P.
; Macias, Leila
; Maciel, Jefferson R.
; Magenta, Mara A.G.
; Mamede, Maria C.H.
; Manoel, Evelin A.
; Marchioretto, Maria S.
; Marques, Juliana S.
; Marquete, Nilda
; Marquete, Ronaldo
; Martinelli, Gustavo
; Martins da Silva, Regina C.V.
; Martins, Ângela B.
; Martins, Erika R.
; Martins, Márcio L.L.
; Martins, Milena V.
; Martins, Renata C.
; Matias, Ligia Q.
; Maya-L., Carlos A.
; Mayo, Simon
; Mazine, Fiorella
; Medeiros, Debora
; Medeiros, Erika S.
; Medeiros, Herison
; Medeiros, João D.
; Meireles, José E.
; Mello-Silva, Renato
; Melo, Aline
; Melo, André L.
; Melo, Efigênia
; Melo, José I.M.
; Menezes, Cristine G.
; Menini Neto, Luiz
; Mentz, Lilian A.
; Mezzonato, A.C.
; Michelangeli, Fabián A.
; Milward-de-Azevedo, Michaele A.
; Miotto, Silvia T.S.
; Miranda, Vitor F.O.
; Mondin, Cláudio A.
; Monge, Marcelo
; Monteiro, Daniele
; Monteiro, Raquel F.
; Moraes, Marta D.
; Moraes, Pedro L.R.
; Mori, Scott A.
; Mota, Aline C.
; Mota, Nara F.O.
; Moura, Tania M.
; Mulgura, Maria
; Nakajima, Jimi N.
; Nardy, Camila
; Nascimento Júnior, José E.
; Noblick, Larry
; Nunes, Teonildes S.
; O'Leary, Nataly
; Oliveira, Arline S.
; Oliveira, Caetano T.
; Oliveira, Juliana A.
; Oliveira, Luciana S.D.
; Oliveira, Maria L.A.A.
; Oliveira, Regina C.
; Oliveira, Renata S.
; Oliveira, Reyjane P.
; Paixão-Souza, Bruno
; Parra, Lara R.
; Pasini, Eduardo
; Pastore, José F.B.
; Pastore, Mayara
; Paula-Souza, Juliana
; Pederneiras, Leandro C.
; Peixoto, Ariane L.
; Pelissari, Gisela
; Pellegrini, Marco O.O.
; Pennington, Toby
; Perdiz, Ricardo O.
; Pereira, Anna C.M.
; Pereira, Maria S.
; Pereira, Rodrigo A.S.
; Pessoa, Clenia
; Pessoa, Edlley M.
; Pessoa, Maria C.R.
; Pinto, Luiz J.S.
; Pinto, Rafael B.
; Pontes, Tiago A.
; Prance, Ghillean T.
; Proença, Carolyn
; Profice, Sheila R.
; Pscheidt, Allan C.
; Queiroz, George A.
; Queiroz, Rubens T.
; Quinet, Alexandre
; Rainer, Heimo
; Ramos, Eliana
; Rando, Juliana G.
; Rapini, Alessandro
; Reginato, Marcelo
; Reis, Ilka P.
; Reis, Priscila A.
; Ribeiro, André R.O.
; Ribeiro, José E.L.S.
; Riina, Ricarda
; Ritter, Mara R.
; Rivadavia, Fernando
; Rocha, Antônio E.S.
; Rocha, Maria J.R.
; Rodrigues, Izabella M.C.
; Rodrigues, Karina F.
; Rodrigues, Rodrigo S.
; Rodrigues, Rodrigo S.
; Rodrigues, Vinícius T.
; Rodrigues, William
; Romaniuc Neto, Sérgio
; Romão, Gerson O.
; Romero, Rosana
; Roque, Nádia
; Rosa, Patrícia
; Rossi, Lúcia
; Sá, Cyl F.C.
; Saavedra, Mariana M.
; Saka, Mariana
; Sakuragui, Cássia M.
; Salas, Roberto M.
; Sales, Margareth F.
; Salimena, Fatima R.G.
; Sampaio, Daniela
; Sancho, Gisela
; Sano, Paulo T.
; Santos, Alessandra
; Santos, Élide P.
; Santos, Juliana S.
; Santos, Marianna R.
; Santos-Gonçalves, Ana P.
; Santos-Silva, Fernanda
; São-Mateus, Wallace
; Saraiva, Deisy P.
; Saridakis, Dennis P.
; Sartori, Ângela L.B.
; Scalon, Viviane R.
; Schneider, Ângelo
; Sebastiani, Renata
; Secco, Ricardo S.
; Senna, Luisa
; Senna-Valle, Luci
; Shirasuna, Regina T.
; Silva Filho, Pedro J.S.
; Silva, Anádria S.
; Silva, Christian
; Silva, Genilson A.R.
; Silva, Gisele O.
; Silva, Márcia C.R.
; Silva, Marcos J.
; Silva, Marcos J.
; Silva, Otávio L.M.
; Silva, Rafaela A.P.
; Silva, Saura R.
; Silva, Tania R.S.
; Silva-Gonçalves, Kelly C.
; Silva-Luz, Cíntia L.
; Simão-Bianchini, Rosângela
; Simões, André O.
; Simpson, Beryl
; Siniscalchi, Carolina M.
; Siqueira Filho, José A.
; Siqueira, Carlos E.
; Siqueira, Josafá C.
; Smith, Nathan P.
; Snak, Cristiane
; Soares Neto, Raimundo L.
; Soares, Kelen P.
; Soares, Marcos V.B.
; Soares, Maria L.
; Soares, Polyana N.
; Sobral, Marcos
; Sodré, Rodolfo C.
; Somner, Genise V.
; Sothers, Cynthia A.
; Sousa, Danilo J.L.
; Souza, Elnatan B.
; Souza, Élvia R.
; Souza, Marcelo
; Souza, Maria L.D.R.
; Souza-Buturi, Fátima O.
; Spina, Andréa P.
; Stapf, María N.S.
; Stefano, Marina V.
; Stehmann, João R.
; Steinmann, Victor
; Takeuchi, Cátia
; Taylor, Charlotte M.
; Taylor, Nigel P.
; Teles, Aristônio M.
; Temponi, Lívia G.
; Terra-Araujo, Mário H.
; Thode, Veronica
; Thomas, W.Wayt
; Tissot-Squalli, Mara L.
; Torke, Benjamin M.
; Torres, Roseli B.
; Tozzi, Ana M.G.A.
; Trad, Rafaela J.
; Trevisan, Rafael
; Trovó, Marcelo
; Valls, José F.M.
; Vaz, Angela M.S.F.
; Versieux, Leonardo
; Viana, Pedro L.
; Vianna Filho, Marcelo D.M.
; Vieira, Ana O.S.
; Vieira, Diego D.
; Vignoli-Silva, Márcia
; Vilar, Thaisa
; Vinhos, Franklin
; Wallnöfer, Bruno
; Wanderley, Maria G.L.
; Wasshausen, Dieter
; Watanabe, Maurício T.C.
; Weigend, Maximilian
; Welker, Cassiano A.D.
; Woodgyer, Elizabeth
; Xifreda, Cecilia C.
; Yamamoto, Kikyo
; Zanin, Ana
; Zenni, Rafael D.
; Zickel, Carmem S
.
Resumo Um levantamento atualizado das plantas com sementes e análises relevantes acerca desta biodiversidade são apresentados. Este trabalho se iniciou em 2010 com a publicação do Catálogo de Plantas e Fungos e, desde então vem sendo atualizado por mais de 430 especialistas trabalhando online. O Brasil abriga atualmente 32.086 espécies nativas de Angiospermas e 23 espécies nativas de Gimnospermas e estes novos dados mostram um aumento de 3% da riqueza em relação a 2010. A Amazônia é o Domínio Fitogeográfico com o maior número de espécies de Gimnospermas, enquanto que a Floresta Atlântica possui a maior riqueza de Angiospermas. Houve um crescimento considerável no número de espécies e nas taxas de endemismo para a maioria dos Domínios (Caatinga, Cerrado, Floresta Atlântica, Pampa e Pantanal), com exceção da Amazônia que apresentou uma diminuição de 2,5% de endemicidade. Entretanto, a maior parte das plantas com sementes que ocorrem no Brasil (57,4%) é endêmica deste território. A proporção de formas de vida varia de acordo com os diferentes Domínios: árvores são mais expressivas na Amazônia e Floresta Atlântica do que nos outros biomas, ervas são dominantes no Pampa e as lianas apresentam riqueza expressiva na Amazônia, Floresta Atlântica e Pantanal. Este trabalho não só quantifica a biodiversidade brasileira, mas também indica as lacunas de conhecimento e o desafio a ser enfrentado para a conservação desta flora.
Abstract An updated inventory of Brazilian seed plants is presented and offers important insights into the country's biodiversity. This work started in 2010, with the publication of the Plants and Fungi Catalogue, and has been updated since by more than 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing an increase of 3% in its species richness in relation to 2010. The Amazon Rainforest is the richest Brazilian biome for Gymnosperms, while the Atlantic Rainforest is the richest one for Angiosperms. There was a considerable increment in the number of species and endemism rates for biomes, except for the Amazon that showed a decrease of 2.5% of recorded endemics. However, well over half of Brazillian seed plant species (57.4%) is endemic to this territory. The proportion of life-forms varies among different biomes: trees are more expressive in the Amazon and Atlantic Rainforest biomes while herbs predominate in the Pampa, and lianas are more expressive in the Amazon, Atlantic Rainforest, and Pantanal. This compilation serves not only to quantify Brazilian biodiversity, but also to highlight areas where there information is lacking and to provide a framework for the challenge faced in conserving Brazil's unique and diverse flora.
https://doi.org/10.1590/2175-7860201566411
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14.
Correlation between chemical composition and sensory properties of Brazilian sugarcane spirits (cachaças)
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Serafim, Felipe A. T.
; Seixas, Fernanda R. F.
; Da Silva, Alexandre A.
; Galinaro, Carlos A.
; Nascimento, Eduardo S. P.
; Buchviser, Silmara F.
; Odello, Luigi
; Franco, Douglas W.
.
A correlação entre a composição química e os dados sensoriais de 28 amostras de cachaças foi investigada através de análise de componentes principais (PCA). Um modelo químico usando análise discriminante linear (LDA) para classificar as amostras de cachaças de acordo com suas qualidades sensoriais foi então elaborado. Este modelo apresentou habilidades preditivas de calibração e validação de 87,4 e 100%, respectivamente, e foi capaz de reconhecer corretamente 7 dentre 9 amostras adicionais, apresentando-se como uma ferramenta alternativa potencial para o reconhecimento das qualidades sensoriais de cachaças.
The correlation between the chemical composition and the sensory data for 28 cachaça samples was investigated using principal component analysis (PCA). A chemical model was then developed using linear discriminant analysis (LDA) to classify the distillate samples according to their sensory qualities. This model presented predictive abilities of calibration and validation of 87.4 and 100%, respectively, and was able to recognize correctly 7 out of 9 additional samples according to their sensory evaluations, showing itself as a potential alternative tool of recognizing cachaça sensory qualities.
https://doi.org/10.5935/0103-5053.20130125
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15.
Structure and peroxidase activity of ferric Streptomyces clavuligerus orf10-encoded protein P450CLA: UV-visible, CD, MCD and EPR spectroscopic characterization
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Goto, Leandro S.
; Hokka, Carlos O.
; Lima, José F.
; Prieto, Tatiana
; Araújo, Ana Paula U.
; Nantes, Iseli L.
; Nascimento, Otaciro R.
.
O presente trabalho reporta a caracterização espectroscópica por absorção UV-visível, dicroísmo circular magnético (MCD) e ressonância paramagnética eletrônica (EPR) da proteína recombinante do tipoP450-canfora (P450CAM) codificada pela orf10 de Streptomyces clavuligerus (P450CLA) expressa por Escherichia coli Rosetta na forma nativa e associada a ligantes externos contendo os grupos β-lactamato, oxazol e derivado de alquilamina (álcool) do ácido clavulâmico. Considerando a diversidade de aplicações da enzima P450, sua reatividade com terc-butil-hidroperóxido (terc-BuOOH) foi caracterizada. O grupo heme da P450CLA apresenta-se covalentemente ligado e exibe características espectrais de UV-visível, CD e MCD de P450CAM, incluindo a banda Soret em 450 nm proveniente do complexo ferroso-CO. P450CLA foi convertido em espécies de valência alta pelo terc-BuOOH e promoveu cisão homolítica da ligação O-O. O perfil radicalar da reação foi terc-butiloxil como radical primário e metil e butilperoxil como radicais secundários. Os radicais metil e butilperoxil resultaram respectivamente da cisão-β do radical alcoxil e da reação do radical metil com oxigênio molecular.
The present study reports the spectroscopic characterization by UV-visible absorption spectroscopy, magnetic circular dichroism (MCD) and electron paramagnetic resonance (EPR) of the recombinant orf10-encoded P450-camphor like protein (P450CLA)of Streptomyces clavuligerus expressed in Escherichia coli Rosetta in the native form and associated to external ligands containing the β-lactam, oxazole and alkylamine-derived (alcohol) moieties of the clavulamic acid. Considering the diversity of potential applications for the enzyme, the reactivity with tert-butylhydroperoxide (tert-BuOOH) was also characterized. P450CLA presents a covalently bound heme group and exhibited the UV-visible, CD and MCD spectral features of P450CAM including the fingerprint Soret band at 450 nm generated by the ferrous CO-complex. P450CLA was converted to high valence species by tert-BuOOH and promoted homolytic scission of the O-O bond. The radical profile of the reaction was tert-butyloxyl as primary and methyl and butylperoxyl as secondary radicals. The secondary methyl and butylperoxyl radicals resulted respectively from the β-scission of the alkoxyl radical and from the reaction of methyl radical with molecular oxygen.
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Glossary and search help
You can enrich your search in a very simple way. Use the search indexes combined with the connectors (AND or OR) and specify more your search.
For example, if you want to search for articles about
cases of dengue in Brasil in 2015, use:ti:dengue and publication_year:2015 and aff_country:Brasil
See below the complete list of search indexes that can be used:
Index code | Element |
---|---|
ti | article title |
au | author |
kw | article keywords |
subject | subject (title words, abstract and keywords) |
ab | abstract |
ta | journal short title (e.g. Cad. Saúde Pública) |
journal_title | journal full title (e.g. Cadernos de Saúde Pública) |
la | publication language code (e.g. pt - Portuguese, es - Spanish) |
type | document type |
pid | publication identifier |
publication_year | publication year of publication |
sponsor | sponsor |
aff_country | country code of the author's affiliation |
aff_institution | author affiliation institution |
volume | article volume |
issue | article issue |
elocation | elocation |
doi | DOI number |
issn | journal ISSN |
in | SciELO colection code (e.g. scl - Brasil, col - Colômbia) |
use_license | article usage license code |