ABSTRACT The objective of this work was to describe morphology and grouping of Paspalum notatum accessions, based on multicategorical data which discards the redundant variables for quantification of genetic diversity. We also tested the hypothesis that geographical distance was correlated with morphological divergence. In our study, multivariate analyzes successfully demonstrated the geographic and morphological variability of the P. notatum accessions characterized. Many of these evaluated accessions can be included in future genetic improvement programs. Based on two methodologies for discarding variables, it was possible to identify the potentially important morphological characteristics from genetic diversity studies and characterize new accessions aimed at improving forage and seed production. The methodologies used to discard variables are biometric tools that can be used successfully in future plant breeding programs, especially when a large number of traits and accessions are being evaluated. Although significant, geographic distance had a low association with morphological traits. This indicated the need to use other characteristics, such as forage and seed yield, in addition to molecular analysis. Our analyzes showed genetic variability in P. notatum for all the characteristics studied.

ABSTRACT The objective of this work was to determine Paspalum notatum genotypes appropriate for the establishment of mixtures with temperate legumes and compare the productivity of mixtures with mineral-fertilized systems along two years. Four hybrids of P. notatum, ecotype Bagual and cv. Pensacola, were either subjected to mixtures with white clover (Trifolium repens) and birdsfoot trefoil (Lotus corniculatus) or fertilized with 0, 60, 120, 240, and 480 kg N ha−1 year−1. P. notatum leaf blades and stems and herbage accumulation denoted a management × year interaction. Herbage accumulation varied between the mixtures and fertilized systems in first year, while in the second year, herbage accumulation of mixed systems employing the B26, B43, C22, C9, and Bagual genotypes were similar to that of systems fertilized with 120 and 240 kg N ha−1. Hybrids B26 and C9 and ecotype Bagual, once made available to producers, could be indicated for mixtures with temperate legumes because they are superior to cv. Pensacola. Herbage accumulation in mixtures involving white clover plus birdsfoot trefoil and P. notatum genotypes is similar to that of mineral nitrogen-fertilized systems with 240 kg N ha−1, emphasizing the viability of mixed between these species.

ABSTRACT The rational use of N fertilization is essential to increase the recovery efficiency and crop productivity and decrease the cost of production. The objective of this study was to assess forage yield (TDM), tillers population density (TPD) and N use efficiency (NUE) in six Paspalum notatum Flüggé genotypes in response to N fertilization. The experimental design involved randomized blocks on a subdivided plot design. In 2014-2015 the TDM was higher for the C22 and B26 hybrids (P < 0.0001), which were similar to each other, with an average of 6173 kg DM ha-1 yr-1. In 2015-2016 the TDM of the genotypes ranging from 7053 to 13773 kg DM ha-1 yr-1, for pastures fertilized with 0 and 480 kg N ha-1 yr-1, respectively. An interaction was found between Genotype × N fertilization level (P = 0.0155) for TDM in 2016-2017. In the years 2015-2016 and 2016-2017 the C22 hybrid standed out as the genotype with the highest tiller production in response to N fertilization. In the year 2014-2015 NUE was higher (P = 0.0015) in the N fertilization levels N60, being of 15.5 kg DM kg-1 N. In the years 2015-2016 and 2016-2017 the NUE was higher at fertilization level N120 (P < 0.05), being of 21.1 and 31.5 kg DM kg-1 N, respectively. The C22 hybrid was distinct as the genotype with the highest DM yield and superior tillering characteristics. The N fertilization level of 120 kg N ha-1 yr-1 promoted greater NUE in all P. notatum genotypes

ABSTRACT Aim To analyze the influence of the -31 C/T polymorphism of the interleukin-1β gene on Helicobacter pylori eradication therapy success in patients with functional dyspepsia. Methods Functional dyspepsia was diagnosed according to the Rome III criteria. All patients underwent upper gastrointestinal endoscopy, and gastric biopsies were obtained at screening and 12 months after randomization (last follow-up visit). Urease test and histological examination were performed to define the H. pylori status. Patients received twice-daily amoxicillin, clarithromycin and omeprazole for 10 days. Genotyping of the interleukin-1beta -31 C/T polymorphism (rs1143627) was performed using polymerase chain reaction-restriction fragment length polymorphism. Results One hundred forty-nine patients received treatment with triple therapy for H. pylori eradication. Only one patient was lost to follow-up, and adherence to study medication was 94.6%. A total of 148 patients (mean age 46.08 ± 12.24 years; 81.8% women) were evaluated for the influence of the interleukin-1beta -31 C/T polymorphism on the outcome of H. pylori eradication therapy. After treatment, bacteria were eradicated in 87% of patients (129/148). Genotype frequencies of the polymorphism were as follows: CC, 38/148 (25.7%); CT, 71/148 (47.9%); and TT, 39/148 (26.4%). Successful eradication rate was 78.9%, 94.4% and 82.1% for the CC, CT and TT genotypes, respectively. The CT genotype was significantly associated with successful H. pylori eradication (p= 0.039). Conclusion This study suggests that the CT genotype of the interleukin-1beta -31 C/T polymorphism plays a role in the successful eradication of H. pylori among patients with functional dyspepsia.

Abstract Functional dyspepsia and lactose intolerance (adult-type hypolactasia, ATH) are common conditions that may coexist or even be confounded. Their clinical presentation can be similar, however, lactose intolerance does not form part of the diagnostic investigation of functional dyspepsia. Studies on the association between functional dyspepsia and ATH are scarce. This study aimed to evaluate whether ATH is associated with symptoms of functional dyspepsia. Patients fulfilling the Rome III diagnostic criteria for functional dyspepsia underwent genetic testing for ATH. Dyspeptic symptoms were evaluated and scored according to a validated questionnaire. The diagnostic criteria for ATH was a CC genotype for the -13910C/T polymorphism, located upstream of the lactase gene. The mean scores for dyspeptic symptoms were compared between patients with ATH and those with lactase persistence. A total of 197 functional dyspeptic patients were included in the study. Mean age was 47.7 years and 82.7% patients were women. Eighty-eight patients (44.7%) had a diagnosis of ATH. Abdominal bloating scores were higher in ATH patients compared to the lactase persistent patients (P=0.014). The remaining dyspeptic symptom scores were not significantly different between the two groups. The study results demonstrate an association between ATH and bloating in patients with functional dyspepsia.

ABSTRACT The Paspalum genus includes several species that are important for livestock in Rio Grande do Sul, such as P. notatum and P. guenoarum, typical of native pastures of the Pampa biome. The aim of this study was to investigate forage production and chemical composition of four ecotypes of these species in relation to the cv. ‘Pensacola’ (P. notatum). Ecotypes of P. guenoarum (Azulão and Baio) and P. notatum (André da Rocha and Bagual) and the cv. ‘Pensacola’ were evaluated for two years, during which four cuts/year were made. The work was carried out under field conditions at the Agronomic Experimental Station of the Federal University of Rio Grande do Sul (30°05’S; 51°39’W), in a completely randomized design. P. guenoarum stood out for higher productivity and greater tolerance to cold; the Azulão ecotype showed more autumn production in relation to the other ecotypes. Crude protein content ranged from 14 (Baio) to 15% (‘Pensacola’); for neutral detergent fiber, the variation was 68 (Azulão) to 71% (‘Pensacola’) and for acid detergent fiber there was a variation of 38 (‘Pensacola’) to 43% (Baio). The data demonstrates the potential of native genotypes for use as cattle feeding in southern Brazil.

RESUMO: A pastagem nativa é uma importante fonte de forragem para produção de bovinos e ovinos no bioma Pampa. A introdução do azevém e a fertilização nitrogenada são alternativas que visam intensificar a exploração pecuária de forma mais sustentável, podendo causar alterações em vários atributos do solo. Este trabalho teve por objetivo avaliar os efeitos de longo prazo de fertilização nitrogenada sobre atributos indicadores da saúde do solo em pastagem nativa com introdução de azevém anual. O experimento consistiu de uma sucessão secundária da pastagem natural submetida ao pastejo contínuo por bovinos e ovinos, com oferta de forragem constante. Em 1996, anteriormente ao inicio do experimento, a área experimental foi calcareada para correção da acidez do solo. O experimento foi então iniciado, sendo constituído da aplicação de três doses de N em cobertura, correspondendo a 0, 100 e 200kg ha-1 ano-1 de N. Em 2010, amostras de solo foram coletadas nas camadas de 0-20 e 20-40cm para análise de C e N total, particulado e associado aos minerais, além de uma área de referência (campo nativo sem pastejo). Análises microbiológicas foram conduzidas nas camadas de 0-5 e 5-10cm. A adubação nitrogenada de longo prazo em solos de campo nativo com introdução de azevém não altera os estoques totais de C e N, mas aumenta a fração particulada de N na dose de 100kg ha-1 ano-1. Em relação à atividade microbiana, o aumento na dose do fertilizante aumenta a respiração dos microrganismos, assim como o N da sua biomassa.

ABSTRACT: Native pastures are of great importance for cattle and sheep nutrition in the Pampa biome. However, due to its low productivity, the Italian ryegrass introduction and the nitrogen (N) fertilization are alternatives proposed to intensify livestock production in a sustainable manner. The objective of this study was to evaluate the effects of long-term N application on soil health indicators in a native pasture with Italian ryegrass introduction in southern Brazil. The experiment consists of a secondary native pasture under continuous grazing and constant herbage allowance. In 1996 experimental area was broadcast limed and the experiment was initiated, testing three N topdressing rates (0, 100 and 200kg N ha-1 year-1). In 2010 soil of experimental and reference area of non grazed native grassland was sampled in the soil layers of 0-20 and 20-40cm. Total, particulate and mineral associated carbon (C) and N stocks were evaluated. Soil microbiological attributes were evaluated in 0-5 and 5-10cm soil layers. The long-term N fertilization in soils with native pasture and Italian ryegrass introduction did not affect total C and N stocks. However, increases in N particulate fraction were seen with 100kg ha-1 year-1 of N rate of fertilization. Furthermore, the increase in N rates increased N microbial biomass and respiration.

Few studies have adequately described the floristic and structural features of natural grasslands associated with shallow basalt soils in southern Brazil. This study was carried out on natural grazing land used for livestock production in the municipality of Santana do Livramento, in the Campanha region of the state of Rio Grande do Sul, Brazil. The aim of the study was to describe the floristic and structural diversity of the area. The floristic list obtained comprises 229 plant taxa from 40 botanical families, with a predominance of the families Poaceae (62), Asteraceae (28), Fabaceae (16) and Cyperaceae (12). The estimated diversity and evenness in the community were 3.00 and 0.874, respectively. Bare soil and rock outcrops accounted for 19.3% of the area, resulting in limited forage availability. Multivariate analysis revealed two well-defined groups among the sampling units. One group showed a high degree of internal aggregation, associated with deep soils, and was characterized by the presence of tussocks, whereas the other was less aggregate and was characterized by prostrate species growing on shallow soil. Ordination analysis indicated a gradient of moisture and of soil depth in the study area, resulting in different vegetation patterns. These patterns were analogous to the vegetation physiognomies described for Uruguayan grasslands. Overall, the grassland community studied is similar to others found throughout southern Brazil, although it harbors more winter forage species. In addition, the rare grass Paspalum indecorum Mez is locally dominant in some patches, behaving similarly to P. notatum Fl., a widespread grass that dominates extensive grassland areas in southern Brazil.

The successful achievement of a highly productive understorey pasture in silvopastoral systems depends on the use of well-adapted forage genotypes, showing good agronomic performance and persistence under shading and grazing. In this study, the herbage dry matter yield (DMY) and nitrogen nutrition dynamics were determined in three native warm-season grasses (Paspalum regnellii, Paspalum dilatatum and Paspalum notatum) and a forage legume (Arachis pintoi) under two shading levels compared with full sun. The experiment was conducted in the Campanha region, Bagé, state of Rio Grande do Sul, Brazil, during two evaluation cycles (2008/2009 and 2009/2010). Three shade cloth levels (0%, 50% and 80% of light restriction) were applied to the forage genotypes in a split plot design, in which shading levels were the main plot and forage genotypes were the subplots, with three replications. P. regnellii showed the highest accumulated DMY (1500 and 1700 g m-2, respectively, for the first and second evaluation cycles) at all shading levels and showed no DMY decreased under the heavy shade (80%). Average DMY over the four genotypes under the 50% shade level was higher or equal compared with full sun. Influence of rainfall was observed on the DMY performance of all genotypes: the positive effect of moderate shading (50%) on P. dilatatum and P. notatum DMY was associated to a low soil water availability status. Increased shading level resulted in high nitrogen nutrition index values on grasses, in comparison with full sun. All genotypes performed well under the moderate shading level, but the DMY of both P. regnellii and P. dilatatum and the herbage N content in P. notatum and A. pintoi of all genotypes stood out, showing that those main genotypes are promising to grow in silvopastoral systems at the Campanha region in southern Brazil.

The objective of this study was to evaluate the chemical composition of the forage apparently consumed by steers in a natural grassland on region of Campanha, in Rio Grande do Sul, Brazil, subjected or not to different inputs: NP - natural pasture without inputs; FNP - fertilized natural pasture and INP - improved natural grassland with fertilization and over-seeded with cultivated winter species. Three Angus steers testers and a variable number of regulator animals per experimental unit were utilized in order to maintain 13 kg of DM/100 kg of live weight (LW) as forage allowance. One time at each season, hand plucking samples were performed along the daily grazing time simulating forage harvested by the animals. The collected samples after drying and grind were submitted to chemical analysis to determine the forage quality. Except in winter and spring, the values of neutral detergent fiber were higher than the critical value of 550 g/kg of DM, which could limit forage intake, demonstrating that the values of forage on offer provided (15.6; 13.7; 13.5; 15.8 kg of DM/100 kg of LW/day in summer, autumn, winter and spring, respectively) were not restrictive to intake. The oversowing of winter cultivated species or fertilization positively alter the degradable fiber content. The seasons had marked influence on the chemical composition of forage apparently consumed; positively increasing some fractions of forage chemical composition in the seasons in which native or cultivated winter species increased their participation. The forage chemical composition is the determining factor in animal performance in natural pasture.

The present study was conducted to assess carcass features, physicochemical and sensory parameters of meat from steers finished on three types of pastures: natural pasture; natural pasture improved, fertilized and oversown with winter species; and annual summer grassland. The experiment was conducted from December 14, 2009 to November 30, 2010, with treatments distributed in a completely randomized design with a different number of replicates. Animals were used as experimental units. Experimental animals were Aberdeen Angus steers with twenty months of initial age and 354±27.4 kg of live weight, on average. The highest average daily gains were obtained for the annual summer grassland. There was no effect of treatments on carcass conformation. The highest carcass yield was obtained on the improved natural pasture. Forequarter yield, side cut yield and longissimus muscle area were similar between the pastures. Moisture and total lipids were not affected by the pasture. Thawing and cooking losses were higher in improved natural pasture and lower in sorghum pasture. Regardless of the treatment, the meat had luminosity ranging from intermediate to dark, high in red, high in yellow, and considered within the normal range for beef. Meat of higher shear force was found in natural pasture, and lower shear force was observed in meat from annual summer grassland. Average live weight daily gain explained 18% of the shear force. Sensory evaluation by duo-trio test showed differences between samples from distinct pastures in flavor. All the studied systems allow for desirable characteristics in carcass and meat.

The overseeding of ryegrass (Lolium multiflorum Lam) on sub-tropical native pastures increases the availability and quality of forage in the cold season. However, soil chemical amendment and nitrogen use can affect the floristic composition, with consequences to the primary and secondary production. In a randomized complete block design with three replications, the effect of nitrogen fertilization (40, 90 and 140 kg/ha of nitrogen) on native pasture overseeded with ryegrass in two consecutive years was assessed regarding production and composition of pasture and performance of beef calves. In both years, the forage accumulation rate and forage production responded linearly to the increase in nitrogen rates as a result of increased participation of ryegrass. The lower efficiency of responses in 2008 was due to the increased participation of dead material and the reduction of ryegrass in the forage sward. In 2007 there was an increasing substitution of the participation of native grasses by ryegrass with increasing nitrogen rates. Native legumes and other species were almost not affected by nitrogen fertilization. The average daily weight gain of the animals responded linearly to nitrogen rates in 2007, and quadratically in 2008. The stocking rate and gain per hectare increased linearly in both years, with greater responses in 2007. The production and composition of forage and livestock production are improved by the use of nitrogen, but the efficiency of responses can be affected by sowing date and nitrogen fertilization.

O objetivo deste trabalho foi avaliar possíveis alterações na densidade e na porosidade total do solo, em razão do pisoteio de cordeiros, sob diferentes manejos, após as culturas de verão em plantio direto. O experimento foi realizado em Argissolo Vermelho distrófico argiloso, nas camadas 0-2,5, 2,5-5 e 5-10 cm, em pastagem de azevém anual manejada com ofertas de forragem de 2,5 ou 5,0 vezes o potencial de consumo dos animais, em lotação contínua e rotacionada. Como testemunha, utilizou-se uma área não pastejada. As intensidades de pastejo e os métodos de pastoreio não tiveram efeito sobre a densidade e a porosidade total do solo. É possível utilizar, durante o inverno, áreas com este tipo de textura do solo, com culturas de cobertura para pastejo de cordeiros, sem que as alterações nos atributos físicos do solo, pelo pisoteio animal, atinjam níveis prejudiciais para as culturas subsequentes.

The objective of this work was to evaluate possible alterations in the soil bulk density and total porosity due to lamb trampling, under different grazing management after no-tillage summer crop. The experiment was carried out in the layers 0-2.5, 2.5-5 e 5-10 cm of an arenic Ultisol, in an annual ryegrass pasture with forage allowance of 2.5 and 5.0 of animal potential consumption, under continuous and rotational stocking. An ungrazed area was used as check. Grazing intensities and methods did not affect soil bulk density and total porosity. It is possible to use areas with this kind of soil texture, with cover crops for lamb grazing in the winter, without alterations on soil physical attributes, caused by animal trampling, reach harmful levels for subsequent crops.

Avaliou-se a viabilidade de abater animais de distintos grupos raciais com até quatro dentes em diferentes tipos de pastagem: natural, adubada, e melhorada por fertilização e sobressemeadura de espécies hiberno-primaveris. O delineamento experimental utilizado foi o de blocos ao acaso, com três repetições. Os dados foram agrupados em estações do ano (inverno e primavera) e submetidos à análise de variância. Foram utilizados novilhos de corte Angus e mestiços, ambos com média de idade inicial de 20 meses. A massa de forragem total e a altura do pasto apresentaram diferenças entre os tipos de pastagem e as estações do ano. A taxa de lotação e a massa de forragem verde sofreram interação entre o tipo de pastagem e a estação do ano. O ganho por área ao longo do experimento foi maior na pastagem adubada (259 kg/ha) e melhorada (263 kg/ha) em comparação à pastagem natural (126 kg/ha). O ganho médio diário foi maior para os novilhos Angus (1,087 kg/dia) e na estação de inverno (1,251 kg/dia). Não houve diferença para a área de olho-de-lombo entre o tipo de pastagem e grupo racial na data do abate. A espessura de gordura subcutânea sofreu interação entre o tipo de manejo e o grupo racial, uma vez que novilhos mestiços depositaram mais gordura na pastagem adubada e novilhos Angus na melhorada. Animais mestiços atingiram menor peso ao abate (505 kg), porém maior rendimento de carcaça (51,6%). Somente os animais mantidos na pastagem natural não atingiram acabamento suficiente para a comercialização. A utilização de fertlizantes e sementes na pastagem natural possibilita o abate de novilhos precoces independente do grupo racial. O ambiente pastoril determina diferentes respostas produtivas conforme a genética utilizada. O cruzamento é uma alternativa para incrementar atributos importantes da carcaça e diminuir o tempo de terminação.

The viability for slaughtering steers of distinct breeds with four teeth at most, on different types of pasture (natural, fertilized and fertilized with overseeding) with winter species was evaluated. The experimental design was randomized blocks with three replicates (padlocks). Data were analyzed according to the seasons (winter and spring) and submitted to analyses of variance. Angus calves and crossbred calves were used, both 20 months of age on average. Total forage mass and the pasture height showed differences between pasture type and seasons. Carrying capacity rate and green forage mass showed interaction between pasture type and season. Yield per unit area during the experimental period was higher in fertilized (259 kg/ha) and oversown pasture (263 kg/ha) than natural pasture (126 kg/ha). Average live-weight gain was higher for the Angus steers (1.087 kg/day) and during the winter (1.251 kg/day). Rib eye area did not show statistical difference between the pasture type and breeds at slaughtering time. Fat thickness showed interaction according to pasture and breeds, where crossbred steers were fatter on fertilized pasture and Angus steers were fatter on oversow pasture. Crossbred animals got lower slaughtering weight (505 kg), yet higher carcass yields (51.6%). Only steers kept on natural pasture did not reach enough fatness for commercialization. The use of fertilizer and seed on the natural pasture allows for slaughtering young steers regardless of the breed. Pasture condition allows different productive performances according to the animal genetics used. Crossbreeding is an alternative to increase important attributes on carcass and to reduce the finishing phase duration.

The objective of this study was to evaluate the vegetal and animal production of a natural pasture on a Mollisol soil in the region of Campanha, in RS. The experimental design consisted of complete randomized blocks with three replicates, which enables the comparison between natural unfertilized pastures with fertilized pastures and pastures fertilized and overseeded with Lolium multiflorum, Lotus corniculatus cv. São Gabriel and Trifolium repens cv. Lucero (NPO). Data were submitted to the analyses of variance considering seasons of the year as time repeated measurements. Aberddeen Angus calves of about nine months of age were used in continuous stocking with variable stocking rate in order to maintain forage offer at 13% of live weight. Data were collected between July 7th (2007) and May 3rd (2008), totaling 302 days. Fertilized and oversown pastures showed higher green forage mass mainly in the spring, as result of higher dry matter (DM) accumulation rate. Average daily live-weight gain for fertilized pasture (0.581 kg/day) was higher than for natural pasture (0.473 kg/day). Higher live-weight gains were obtained in the fall (0.869 kg/day). Stocking rate showed interaction with the seasons of the year, with higher stocking rates obtained in oversown pasture (701 kg of LW/ha) and fertilized pasture (667 kg of LW/ha) during the spring. Live-weight gain per hectare from natural pasture (224 kg LW/ha) was lower than on fertilized (310 kg LW/ha) and over-sown pasture (287 kg LW/ha). Forage mass, dry matter content, forage allowance and pasture height explained 61% of the average live weight gain. Despite the good performance of native grasslands in this region in its natural condition, the utilization of different inputs as fertilization and oversowing of winter cultivated species promoted positive differences in forage production and its distribution along the year and in animal yield.