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Assessment of the regenerative potential of macro-porous chitosan-calcium simvastatin scaffolds on bone cells
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GALLINARI, Marjorie de Oliveira

; BORDINI, Ester Alves Ferreira

; STUANI, Vitor de Toledo

; CASSIANO, Fernanda Balestrero

; MELO, Camila Correa da Silva Braga de

; ALMEIDA, Juliano Milanezi de

; CINTRA, Luciano Tavares Ângelo

; DE SOUZA COSTA, Carlos Alberto

; SOARES, Diana Gabriela

Brazilian Oral Research

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2023, Volume 37 elocation e018

Abstract: En | Text: En | PDF: En

Abstract This study evaluated the bioactive potential of a macro-porous chitosan scaffold incorporated with calcium hydroxide (CH-Ca) and functionalized with bioactive doses of simvastatin (SV) for bone tissue regeneration. Initially, the bioactive dose of SV in osteoblastic cells (SAOS-2) was determined. For the direct contact experiment, SAOS-2 cells were plated on scaffolds to assess cell viability and osteogenic differentiation. The second assay was performed at a distance using extracts from scaffolds incubated in culture medium to assess the effect of conditioned medium on viability and osteogenic differentiation. The initial screening showed that 1 μM SV presented the best biostimulating effects, and this dose was selected for incorporation into the CH-Ca and pure chitosan (CH) scaffolds. The cells remained viable throughout the direct contact experiment, with the greatest cell density in the CH-Ca and CH-Ca-SV scaffolds because of their higher porosity. The CH-Ca-SV scaffold showed the most intense bio-stimulating effect in assays in the presence and absence of osteogenic medium, leading to an increased deposition of mineralized matrix. There was an increase in the viability of cells exposed to the extracts for CH-Ca, CH-SV, and CH-Ca-SV during the one-day period. There was an increase in ALP activity in the CH-Ca and CH-Ca-SV; however, the CH-Ca-SV scaffold resulted in an intense increase in the deposition of mineralized nodules, approximately 56.4% at 7 days and 117% at 14 days, compared with CH (control). In conclusion, functionalization of the CH-Ca scaffold with SV promoted an increase in bioactivity, presenting a promising option for bone tissue regeneration.

https://doi.org/10.1590/1807-3107bor-2023.vol37.0018

Estimation of leaf nitrogen levels in sugarcane using hyperspectral models
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Barros, Pedro Paulo da Silva

; Fiorio, Peterson Ricardo

; Demattê, José Alexandre de Melo

; Martins, Juliano Araújo

; Montezano, Zaqueu Fernando

; Dias, Fábio Luis Ferreira

Ciência Rural

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2022, Volume 52 N. 7 elocation e20200630

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A cana-de-açúcar se destaca como uma das fontes de energia renovável frente às estratégias para reduzir a emissão de gases causadores do efeito estufa. O nitrogênio é um dos mais significativos devido ao seu impacto no crescimento de folhas e colmos. Portanto, o monitoramento eficiente do nitrogênio aplicado é essencial e o sensoriamento remoto se apresenta como uma alternativa na melhoria do gerenciamento da adubação. O presente trabalho teve por objetivo selecionar comprimentos de onda a partir de dados hiperespectrais de dossel da cana-de-açúcar para geração de modelos na predição da concentração de Nitrogênio. O estudo foi realizado em experimentos de campo instalados nos municípios de Piracicaba, Jaú e Santa Maria da Serra, estado São Paulo, na safra 2013/2014. Cada experimento foi alocado em blocos ao acaso, com parcelas subdivididas e quatro repetições, com variedades de cana-de-açúcar na parcela (três variedades por local, sendo a SP 81-3250 comum à todos) e doses de nitrogênio (0, 50, 100 e 150 kg.ha-1) na subparcela. Na seleção dos comprimentos de onda que melhor se correlacionam com o TFN foi utilizada a metodologia sPLS. Posteriormente, foi realizada a combinação linear dos comprimentos de onda selecionados pela metodologia sPLS, por meio de Regressão Linear Múltipla por Stepwise (SMLR). Foram identificadas bandas importantes nas regiões do visível (700 a 705 nm), red-edge(710 a 720 nm), infravermelho próximo (725, 925, 955 e 980 nm) e infravermelho de ondas curtas (1355, 1420, 1595, 1600, 1605 e 1610 nm). O modelo de predição de TFN teve valores de R² de 0,50 e o RMSE de 1,67 g.kg-1. Os modelos gerados para Piracicaba, Jaú e Santa Maria obtiveram R² ajustados e RMSE, respectivamente, de 0,31 considerado não confiável (1,30 g.kg-1), 0,53 (1,96 g.kg-1) e 0,54 (1,46 g.kg-1). Os sensores hiperespectrais de dossel podem ser utilizados para predição do TFN e monitoramento de aplicação de nitrogênio em cana-de-açúcar.

ABSTRACT: Sugarcane is a good source of renewable energy and helps reduce the emission of greenhouse gases. Nitrogen has a critical role in plant growth; therefore,estimating nitrogen levels is essential, and remote sensing can improve fertilizer management. This field study selects wavelengths from hyperspectral data on a sugarcane canopy to generate models for estimating leaf nitrogen concentrations. The study was carried out in the municipalities of Piracicaba, Jaú, and Santa Maria da Serra, state of São Paulo, in the 2013/2014 growing season. The experiments were carried out using a completely randomized block design with split plots (three sugarcane varieties per plot [variety SP 81-3250 was common to all plots] and four nitrogen concentrations [0, 50, 100, and 150 kgha-1] per subplot) and four repetitions. The wavelengths that best correlated with leaf nitrogen were selected usingsparse partial least square regression. The wavelength regionswere combinedby stepwise multiple linear regression. Spectral bands in the visible (700-705 nm), red-edge (710-720 nm), near-infrared (725, 925, 955, and 980 nm), and short-wave infrared (1355, 1420, 1595, 1600, 1605, and 1610 nm) regions were identified. The R² and RMSE of the model were 0.50 and 1.67 g.kg-1, respectively. The adjusted R² and RMSE of the models for Piracicaba, Jaú, and Santa Maria were 0.31 (unreliable) and 1.30 g.kg-1, 0.53 and 1.96 g.kg-1, and 0.54 and 1.46 g.kg-1, respectively. Our results showed that canopy hyperspectral reflectance can estimate leaf nitrogen concentrations and manage nitrogen application in sugarcane.

https://doi.org/10.1590/0103-8478cr20200630

Posicionamento Brasileiro sobre o Uso da Multimodalidade de Imagens na Cardio-Oncologia – 2021
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Melo, Marcelo Dantas Tavares de

; Paiva, Marcelo Goulart

; Santos, Maria Verônica Câmara

; Rochitte, Carlos Eduardo

; Moreira, Valéria de Melo

; Saleh, Mohamed Hassan

; Brandão, Simone Cristina Soares

; Gallafrio, Claudia Cosentino

; Goldwasser, Daniel

; Gripp, Eliza de Almeida

; Piveta, Rafael Bonafim

; Silva, Tonnison Oliveira

; Santo, Thais Harada Campos Espirito

; Ferreira, Waldinai Pereira

; Salemi, Vera Maria Cury

; Cauduro, Sanderson A.

; Barberato, Silvio Henrique

; Lopes, Heloísa M. Christovam

; Pena, José Luiz Barros

; Rached, Heron Rhydan Saad

; Miglioranza, Marcelo Haertel

; Pinheiro, Aurélio Carvalho

; Vrandecic, Bárbara Athayde Linhares Martins

; Cruz, Cecilia Beatriz Bittencourt Viana

; Nomura, César Higa

; Cerbino, Fernanda Mello Erthal

; Costa, Isabela Bispo Santos da Silva

; Coelho Filho, Otavio Rizzi

; Carneiro, Adriano Camargo de Castro

; Burgos, Ursula Maria Moreira Costa

; Fernandes, Juliano Lara

; Uellendahl, Marly

; Calado, Eveline Barros

; Senra, Tiago

; Assunção, Bruna Leal

; Freire, Claudia Maria Vilas

; Martins, Cristiane Nunes

; Sawamura, Karen Saori Shiraishi

; Brito, Márcio Miranda

; Jardim, Maria Fernanda Silva

; Bernardes, Renata Junqueira Moll

; Diógenes, Tereza Cristina

; Vieira, Lucas de Oliveira

; Mesquita, Claudio Tinoco

; Lopes, Rafael Willain

; Segundo Neto, Elry Medeiros Vieira

; Rigo, Letícia

; Marin, Valeska Leite Siqueira

; Santos, Marcelo José

; Grossman, Gabriel Blacher

; Quagliato, Priscila Cestari

; Alcantara, Monica Luiza de

; Teodoro, José Aldo Ribeiro

; Albricker, Ana Cristina Lopes

; Barros, Fanilda Souto

; Amaral, Salomon Israel do

; Porto, Carmen Lúcia Lascasas

; Barros, Marcio Vinícius Lins

; Santos, Simone Nascimento dos

; Cantisano, Armando Luís

; Petisco, Ana Cláudia Gomes Pereira

; Barbosa, José Eduardo Martins

; Veloso, Orlando Carlos Glória

; Spina, Salvador

; Pignatelli, Ricardo

; Hajjar, Ludhmilla Abrahão

; Kalil Filho, Roberto

; Lopes, Marcelo Antônio Cartaxo Queiroga

; Vieira, Marcelo Luiz Campos

; Almeida, André Luiz Cerqueira

Arquivos Brasileiros de Cardiologia

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oct 2021, Volume 117 N. 4 Pages 845 - 909

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20200266 103 downloads

Potential use of hyperspectral data to monitor sugarcane nitrogen status
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Martins, Juliano Araujo

; Fiorio, Peterson Ricardo

; Barros, Pedro Paulo da Silva

; Demattê, José Alexandre Melo

; Molin, José Paulo

; Cantarella, Heitor

; Neale, Christopher Michael Usher

Acta Scientiarum. Agronomy

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2021, Volume 43 elocation e47632

Abstract: En | Text: En | PDF: En

ABSTRACT. Nitrogen management in crops is a key activity for agricultural production. Methods that can determine the levels of this element in plants in a quick and non-invasive way are extremely important for improving production systems. Within several fronts of study on this subject, proximal and remote sensing methods are promising techniques. In this regard, this research sought to demonstrate the relationships between variations in leaf nitrogen content (LNC) and sugarcane spectral behaviour. The work was carried out in three experimental areas in São Paulo State, Brazil, with different soils, varieties and nitrogen rates during the 2012/13 and 2013/14 seasons. A significant correlation was observed between the LNC and variations in the sugarcane spectra. The green and red-edge spectral bands were the most consistent and stable predictors of LNC among the evaluated harvests. Stepwise multiple linear regression analysis (MSLR) generated better models for LNC estimation when calibrated with experimental area, independent of the variety. The present research demonstrates that specific wavelengths are associated with the variation in LNC in sugarcane, and these are reported in the green region (near 550 nm) and in the red-edge wavelengths (680 to 720 nm). These results may help in future research on the direct in situ application of nitrogen fertilizers.

https://doi.org/10.4025/actasciagron.v43i1.47632 500 downloads

Avaliação de amêndoas de baru in natura armazenadas em diferentes embalagens
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Reis, Vanesa Beny da Silva Xavier

; Campos, André José de

; Araujo, Kari Katiele Souza

; Melo, Pâmella de Carvalho

; Reis, Juliano de Lima

Revista de Ciências Agrárias

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jun 2019, Volume 42 N. 2 Pages 261 - 270

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A amêndoa de baru (Dipteryx alata Vog.), é uma importante fonte de nutrientes e uma alternativa culinária para a confeção de diversos pratos e inserção em dietas saudáveis. O seu uso e comercialização contribui para a preservação do bioma Cerrado, porém a falta de estudos e conhecimento inerentes à sua conservação no período pós-colheita pode limitar a sua utilização e conduzir a relevantes perdas. Dessa forma, o objetivo deste trabalho foi avaliar as características físicas e químicas na qualidade e conservação das amêndoas de baru in natura. Foram avaliados o ganho de massa, firmeza, sólidos solúveis totais, pH e acidez titulável de amêndoas, em 5 tipos de embalagens: polipropileno (PP), cloreto de polivinila + poliestireno expandido (PVC + EPS), polietileno de baixa densidade (PEBD), polietileno tereftalato (PET) e controle), armazenadas em B.O.D. a 10±2°C. A embalagem PP apresentou os menores valores de ganho de massa e de acidez titulável. As embalagens PP e PVC + EPS obtiveram valores superiores às demais para pH no final do armazenamento, não sendo constatada variação significativa para a firmeza durante o armazenamento. Com base nos resultados, conclui-se que amêndoas de baru in natura podem ser conservadas por refrigeração durante 42 dias, sendo que, dentre as embalagens estudadas, as que apresentaram os parâmetros mais favoráveis foram PP e PVC + EPS.

The almond of baru (Dipteryx alata Vog.) is an important source of nutrients and a culinary alternative for making healthy dishes and diets. Its use and archiving for conservation of the Cerrado biome, but a failure of studies and practices inherent to its life in case of exclusion can limit its use and lead to damages to knowledge. In this way, the present work had as objective to evaluate as physical and technical characteristics in the quality and conservation of uncooked baru almonds. It was evaluated the mass gain, firmness, total soluble solids, pH and titratable acidity of almonds in 5 types of condensation polyethylene (PP), polyvinyl chloride + expanded polystyrene (PVC + EPS), low density polyethylene (LDPE) , polyethylene terephthalate (PET) and control) stored in BOD at 10 ± 2 ° C. The PP packaging is composed of small values of mass gain and titratable acidity. The PP and PVC + EPS packages obtained values similar to and higher than the pH values without end of departure, and no significant change was observed for a firmness during storage. Based on the results, it can be concluded that in-kind clay almonds can be preserved by refrigeration for 42 days. Among the packages studied, the most favorable are PP and PVC + EPS.

https://doi.org/10.19084/rca.17173 790 downloads

ANTHROPOMETRIC AND DIETETIC EVALUATION OF PEOPLE WITH ILEOSTOMIES
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MORAES, Juliano Teixeira

; MELO, Ana Flávia Faria

; ARAÚJO, Camila

; FARIA, Rafaela das Graças Santiago

; FERREIRA, Nize Renê

; BELO, Vinícius Silva

Arquivos de Gastroenterologia

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mar 2019, Volume 56 N. 1 Pages 34 - 40

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO CONTEXTO: Além do manejo adequado das eliminações da estomia, a manutenção da nutrição para a pessoa com ileostomia é um grande desafio. OBJETIVO: Este trabalho teve como objetivo avaliar o estado nutricional de pessoas com ileostomia. MÉTODOS: Trata-se de um estudo transversal, realizado numa população de 17 pessoas com ileostomia cadastrados no Serviço de Atenção à Saúde da Pessoa Ostomizada de nível II referência para uma região de saúde em Minas Gerais. Foi realizada a avaliação antropométrica que consistiu na coleta do peso, altura e cálculo do índice de massa corporal. Também foi avaliado um recordatório alimentar de 24 horas e o método de avaliação nutricional Avaliação Subjetiva Global. Os dados foram analisados pelo software Dietbox, onde foi estimado os nutrientes ingeridos, e pelo Statistical Package for the Social Sciences versão 22.0. RESULTADOS: Houve predomínio de uma população composta por pessoas com idade superior a 50 anos, do sexo feminino, de estado civil casado e solteiro, com renda familiar entre dois e três salários mínimos, ensino fundamental incompleto e que não exerciam atividade remunerada. O câncer foi dado como principal diagnóstico que originou a estomia e possuíam um tempo de cirurgia menor que dois anos. Quanto ao estado nutricional, a maior parte dos entrevistados apresentaram índice de massa corporal adequada, porém mais da metade referiu perda de peso importante após a confecção da ileostomia. O diagnóstico nutricional predominante, fornecido pela Avaliação Subjetiva Geral foi de “suspeita de desnutrição/desnutrido moderado”. Já o aporte de energia, carboidratos, lipídeos, sódio, potássio, magnésio e vitamina B12 foi avaliado como insuficiente na quase totalidade dos participantes. CONCLUSÃO: Portanto, sugere-se a avaliação periódica do estado nutricional dessa população. Assim, espera-se que o diagnóstico e planejamento dietético preventivo, terapêutico e de manutenção possam contribuir para a condição nutricional da pessoa com ileostomia.

ABSTRACT BACKGROUND: The maintenance of nutrition for the person with ileostomy is a major challenge despite to the proper management of stoma eliminations. OBJECTIVE: This study aimed to evaluate the nutritional status of people with ileostomy. METHOD: This is a cross-sectional study carried out on a population of 17 people with ileostomy enrolled in the Service of Attention to the Health of the Person with Stoma of level II, referring to a health region in Minas Gerais. The anthropometric evaluation consisted of the collection of weight, height, and calculation of body mass index. A 24-hour food recall and the nutritional assessment method Global Subjective Assessment were also evaluated. The data were analyzed by the Dietbox software, where the ingested nutrients were estimated, and by the Statistical Package for the Social Sciences version 22.0. RESULTS: There was a predominance of a population composed of people over 50 years old, female, married and single, with family income between two and three minimum wages, incomplete elementary school and that did not exercise paid activity. The cancer was given as the main diagnosis that originated the stoma and had a surgery time of less than two years. Regarding nutritional status, most of the interviewees presented adequate body mass index, but more than half reported significant weight loss after the ileostomy. The predominant nutritional diagnosis provided by the General Subjective Assessment was “suspected malnutrition/moderate malnutrition”. On the other hand, the contribution of energy, carbohydrates, lipids, sodium, potassium, magnesium and vitamin B12 was considered insufficient in almost all the participants. CONCLUSION: Therefore, it is suggested the periodic evaluation of the nutritional status of this population. Thus, it is expected that preventive, therapeutic and maintenance dietary diagnosis and planning may contribute to the nutritional status of the person with the ileostomy.

https://doi.org/10.1590/s0004-2803.201900000-07 1941 downloads

Epidemiological survey of hepatitis C in a region considered to have high prevalence: the state of Minas Gerais, Brazil
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Gomide, Geisa Perez Medina

; Melo, Camila Borges de

; Santos, Vanduí da Silva

; Salge, Vanessa Dib

; Camargo, Fernanda Carolina

; Pereira, Gilberto de Araújo

; Cabral, Sônia Cançado de Oliveira

; Molina, Rodrigo Juliano

; Oliveira, Cristina da Cunha Hueb Barata de

Revista da Sociedade Brasileira de Medicina Tropical

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2019, Volume 52 elocation e20190202

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION: The prevalence of hepatitis C virus (HCV) infection is affected by demographic, virological, clinical, and lifestyle-related factors and varies in different regions in Brazil or worldwide. The present study aimed to clarify the epidemiological patterns of HCV infection in the interior region of Brazil. METHODS: This study was conducted in the Southern Triangle Macro-region of the state of Minas Gerais, Brazil, according to the guidelines of the National Program for the Prevention and Control of Viral Hepatitis. The participants answered a structured questionnaire on social and epidemiological factors. Immunochromatographic rapid tests were used for the qualitative detection of antibodies against HCV in whole blood (Alere HCV® Code 02FK10) in adult subjects by a free-standing method. RESULTS: Of 24,085 tested individuals, 184 (0.76%) were anti-HCV positive. The majority of anti-HCV-positive individuals were born between 1951 and 1980 (n=146 [79.3%]), with 68 women and 116 men. Identified risk factors included syringe and/or needle sharing (p = 0.003), being in prison (p = 0.004), and having tattoos or piercings (p = 0.005) and were significantly associated with the decade of birth. CONCLUSIONS: The study shows the importance of testing populations at risk for HCV infection, including incarcerated individuals, those with tattoos or piercings, those who share or have shared syringes or needles, and those in high-risk birth cohorts (1950s, 1960s, and 1970s) in the Southern Triangle Macro-region.

https://doi.org/10.1590/0037-8682-0202-2019 1209 downloads

Surto de doença transmitida por alimento em evento de massa de populações indígenas em Cuiabá, Mato Grosso, Brasil, no ano de 2013
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Nunes, Daniele Monteiro

; Paula Júnior, Francisco José de

; Melo, Juliano Silva

; De-Oliveira, Elaine Cristina

; Meneguini, Valéria Cristhian

; Dias, Flávia

; Weissheimer, Fábio Liberali

; Dimech, George Santiago

Epidemiologia e Serviços de Saúde

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mar 2016, Volume 25 N. 1 Pages 195 - 202

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo OBJETIVO: relatar a experiência da investigação do surto de doença diarreica aguda (DDA) ocorrido nos XII Jogos dos Povos Indígenas, no município de Cuiabá, estado de Mato Grosso, Brasil, em 2013. MÉTODOS: foram coletados dados das fichas de atendimento dos casos de DDA, definidos como 'indivíduo participante dos Jogos, referindo episódio de diarreia e/ou vômito' no Posto Médico Avançado; foram calculadas a taxa de ataque de DDA, frequências relativas e medidas de tendência central das variáveis sociodemográficas e clínicas, das inspeções sanitárias e dos resultados de amostras bromatológicas. RESULTADOS: houve 384 (37%) casos que atenderam a definição de DDA; os picos epidêmicos do surto corresponderam ao quarto e sétimo dias do evento, e a taxa de ataque da doença foi de 33,5%; a inspeção sanitária mostrou indícios de contaminação alimentar por Staphyloccocus coagulase-negativa, Bacillus cereus e coliformes termotolerantes. CONCLUSÃO: houve surto de DDA causado por contaminação alimentar.

Abstract OBJECTIVE: to report the experience of investigating the outbreak of acute diarrhoea (AD) at the XII Indigenous Games in Cuiabá, Mato Grosso, Brazil, 2013. METHODS: data were collected from the Advanced Medical Post's service records of the AD cases, which were defined as 'individual Games participant referring episode of diarrhoea and/or vomiting'; AD attack rates, relative frequencies and measures of the central tendency of sociodemographic and clinical variables, sanitary inspections and results of bromatological samples were calculated. RESULTS: 384 (37%) cases met the definition of AD; the epidemic peaks of the outbreak occurred on the 4th and 7th day of the event and the disease attack rate was 33.5%; sanitary inspection showed evidence of food contamination by coagulase-negative Staphylococci, Bacillus cereus and heat resistant coliforms. CONCLUSION: there an outbreak of AD caused by food contamination.

Resumen OBJETIVO: presentar la experiencia de investigación del brote de enfermedad diarreica aguda (EDA) que se produjo en los XII Juegos Indígenas en Cuiabá, Mato Grosso, Brasil, 2013. MÉTODOS: se recogieron datos de los registros de atención de los casos de EDA, definido como "individuo participante de los Juegos, que refirió un episodio diarreico y/o vómitos" del puesto médico avanzado; se calculó la tasa de ataque por EDA, frecuencias relativas y medidas de tendencia central de las variables sociodemográficas, inspecciones clínicas, sanitarias y los resultados de las muestras bromatológicos. RESULTADOS: hubo 384 (37%) casos que cumplieron la definición de EDA; los picos epidémico del brote se produjeron en el día cuatro y siete del evento y la tasa de ataque fue de 33,5%; la inspección sanitaria mostró evidencia de contaminación de los alimentos por Staphylococcus coagulase negativo, Bacilluscereus y coliformes. CONCLUSIÓN: hubo un brote de DDA por contaminación alimentaria.

https://doi.org/10.5123/s1679-49742016000100021 5294 downloads

Surto de doença transmitida por alimento em evento de massa de populações indígenas em Cuiabá, Mato Grosso, Brasil, no ano de 2013
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Nunes, Daniele Monteiro

; Paula Júnior, Francisco José de

; Melo, Juliano Silva

; De-Oliveira, Elaine Cristina

; Meneguini, Valéria Cristhian

; Dias, Flávia

; Weissheimer, Fábio Liberali

; Dimech, George Santiago

Epidemiologia e Serviços de Saúde

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mar 2016, Volume 25 N. 1 Pages 195 - 202

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

https://doi.org/10.5123/s1679-49742016000100021 693 downloads

10.

Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
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Bertassoli, Bruno Machado

; Costa, Emanuela Silva

; Sousa, Cristiane Aparecida

; Albergaria, Juliano Douglas Silva

; Maltos, Kátia L. Melo

; Goes, Alfredo Miranda

; Matins, Thais Maria da Mata

; Silva, Gerluza Aparecida Borges

; Jorge, Erika Cristina

Brazilian Archives of Biology and Technology

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2016, Volume 59 elocation e16150613

Abstract: En | Text: En | PDF: En

ABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34 and CD45. Nanog expression was detected by RT-PCR, expected for a mesenchymal stem cell profile. DPSC chondrogenic differentiation was confirmed by positive staining in Alcian Blue; lipidic droplets stained with oil red confirmed their capacity to differentiate in adipogenic fate; while mineralized beads, stained with alizarin red, confirmed their differentiation in osteogenic phenotype. These results indicate the viability of the isolation and expansion of rat DPSC following this method, and osteogenic differentiation potential opens new perspectives for in vivo studies and the use of these cells in cellular therapies and tissue bioengineering, aiming bone repair.

https://doi.org/10.1590/1678-4324-2016150613 2956 downloads

11.

Análise e validação de modelos probabilísticos de malignidade de nódulo pulmonar solitário em uma população no Brasil
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Melo, Cromwell Barbosa de Carvalho

; Perfeito, João Aléssio Juliano

; Daud, Danilo Félix

; Costa Júnior, Altair da Silva

; Santoro, Ilka Lopes

; Leão, Luiz Eduardo Villaça

Jornal Brasileiro de Pneumologia

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oct 2012, Volume 38 N. 5 Pages 559 - 565

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Analisar características clínicas e radiográficas que influenciaram o diagnóstico anatomopatológico de nódulo pulmonar solitário (NPS) e comparar/validar dois modelos probabilísticos de malignidade do NPS em pacientes com NPS no Brasil. MÉTODOS: Análise retrospectiva de 110 pacientes com diagnóstico de NPS submetidos à ressecção em um hospital terciário no período entre 2000 e 2009. As características clínicas estudadas foram gênero, idade, presença de comorbidades sistêmicas, história de neoplasia maligna ao diagnóstico de NPS, diagnóstico histopatológico do NPS, tabagismo, carga tabágica e tempo de cessação do tabagismo. As características radiográficas avaliadas em relação ao NPS foram presença de margens espiculadas, tamanho do maior diâmetro transversal e localização anatômica do NPS. Foram utilizados dois modelos matemáticos, criados em 1997 e 2007, respectivamente, para determinar a probabilidade de malignidade do NPS. RESULTADOS: Houve associações significantes entre malignidade do NPS e idade (p = 0,006; OR = 5,70 para idade >70 anos), presença de margens espiculadas (p = 0,001) e diâmetro maior do NPS (p = 0,001; OR = 2,62 para diâmetro >20 mm). O modelo probabilístico de 1997 mostrou-se superior ao de 2007 - área sob a curva [ASC] ROC = 0,79 ± 0,44 (IC95%: 0,70-0,88) vs. ASC = 0,69 ± 0,50 (IC95%: 0,59-0,79). CONCLUSÕES: Idade elevada, maior diâmetro do NPS e presença de margens espiculadas tiveram associações significantes ao diagnóstico de malignidade do NPS. Nossa análise mostrou que, embora os dois modelos matemáticos sejam eficazes na determinação de malignidade do NPS nessa população, o modelo de 1997 mostrou-se superior.

OBJECTIVE: To analyze clinical and radiographic findings that influence the pathological diagnosis of solitary pulmonary nodule (SPN) and to compare/validate two probabilistic models for predicting SPN malignancy in patients with SPN in Brazil. METHODS: This was a retrospective study involving 110 patients diagnosed with SPN and submitted to resection of SPN at a tertiary hospital between 2000 and 2009. The clinical characteristics studied were gender, age, presence of systemic comorbidities, history of malignancy prior to the diagnosis of SPN, histopathological diagnosis of SPN, smoking status, smoking history, and time since smoking cessation. The radiological characteristics studied, in relation to the SPN, were presence of spiculated margins, maximum transverse diameter, and anatomical location. Two mathematical models, created in 1997 and 2007, respectively, were used in order to determine the probability of SPN malignancy. RESULTS: We found that SPN malignancy was significantly associated with age (p = 0.006; OR = 5.70 for age > 70 years), spiculated margins (p = 0.001), and maximum diameter of SPN (p = 0.001; OR = 2.62 for diameters > 20 mm). The probabilistic model created in 1997 proved to be superior to that created in 2007-area under the ROC curve (AUC), 0.79 ± 0.44 (95% CI: 0.70-0.88) vs. 0.69 ± 0.50 (95% CI: 0.59-0.79). CONCLUSIONS: Advanced age, greater maximum SPN diameter, and spiculated margins were significantly associated with the diagnosis of SPN malignancy. Our analysis shows that, although both mathematical models were effective in determining SPN malignancy in our population, the 1997 model was superior.

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12.

Qualidade de luz e fitorreguladores na multiplicação e enraizamento in vitro da amoreira-preta 'Xavante'
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Pasa, Mateus da Silveira

; Carvalho, Geniane Lopes

; Schuch, Márcia Wulff

; Schmitz, Juliano Dutra

; Torchelsen, Marcela de Melo

; Nickel, Gabriela Kaltbach

; Sommer, Laura Reisdorfer

; Lima, Thaís Santos

; Camargo, Samila Silva

Ciência Rural

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aug 2012, Volume 42 N. 8 Pages 1392 - 1396

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A técnica de micropropagação apresenta diversas vantagens em relação aos métodos tradicionalmente utilizados na propagação da amoreira-preta, especialmente quanto à maior sanidade das mudas e maior rapidez na obtenção de novas plantas. O objetivo deste trabalho foi verificar a influência dos fitorreguladores 6-benzilaminopurina (BAP) e ácido indolbutírico (AIB), aliados à qualidade de luz, na multiplicação e enraizamento in vitro, respectivamente, da amoreira-preta cultivar 'Xavante'. O delineamento experimental utilizado foi inteiramente casualizado. Os tratamentos constituíram-se de diferentes níveis de qualidade de luz (azul, vermelha, branca e ausência de luz) combinadas com ausência ou presença dos fitorreguladores AIB (0,5mg L-1) e BAP (0,8mg L-1) no meio de cultivo. A utilização de BAP aumenta o número de brotações, gemas e folhas da amoreira-preta 'Xavante' e, dessa forma, é indicada para sua multiplicação in vitro. A utilização de AIB não é necessária no enraizamento in vitro da amoreira-preta 'Xavante', indicando que essa cultivar apresenta bom enraizamento natural.

There are a lot of advantages using micropropagation technique over the methods traditionally used in blackberry propagation especially concerning health of seedlings and speed in getting new plants. The aim of this study was to investigate the influence of bioregulators 6-benzylaminopurine (BAP) and indol-3-butyric acid (IBA) combined with light quality on in vitro multiplication and rooting, respectively, of blackberry 'Xavante'. The experimental design was completely randomized. The treatments consisted of different levels of light quality (blue, red, white and absence of light) combined with the absence or presence of bioregulators IBA (0.5mg L-1) and BAP (0.8mg L-1) in the culture medium. The use of BAP increases the number of new shoots, buds and leaves of blackberry, 'Xavante' and thus is suitable for its in vitro propagation. The use of IBA is not required in in vitro rooting of the blackberry cultivar studied, showing that it is naturally well rooting.

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