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RESUMO Os medicamentos reaproveitados são importantes em contextos de recursos limitados porque as intervenções estão mais rapidamente disponíveis, já foram testadas com segurança em outras populações e são, em geral, mais baratas. Os medicamentos reaproveitados são uma solução eficaz, especialmente para doenças emergentes, como a COVID-19. O estudo REVOLUTIOn visa avaliar três medicamentos antivirais reaproveitados: atazanavir, daclatasvir e sofosbuvir, já utilizados em pacientes infectados pelo HIV ou pelo vírus da hepatite C, em um estudo randomizado, controlado por placebo, adaptativo, multibraço e em múltiplos estágios. Os medicamentos serão testados simultaneamente em um ensaio de Fase II para primeiro identificar se algum deles, isoladamente ou em combinação, reduz a carga viral. Se reduzirem, será iniciado um estudo de Fase III para investigar se tais medicamentos são capazes de aumentar o número de dias sem suporte respiratório. Os participantes devem ser adultos hospitalizados com idade ≥ 18 anos com início dos sintomas ≤ 9 dias e saturação de oxigênio ≤ 94% em ar ambiente ou necessidade de oxigênio suplementar para manter saturação de oxigênio > 94%. O tamanho total esperado da amostra varia entre 252 e 1.005 participantes, dependendo do número de estágios que serão concluídos no estudo. Assim, o protocolo é aqui descrito em detalhes, juntamente do plano de análise estatística. Em conclusão, o estudo REVOLUTIOn foi concebido para fornecer evidências se o atazanavir, o daclatasvir ou o sofosbuvir reduzem a carga viral de SARS-CoV-2 em pacientes com COVID-19 e aumentam o número de dias em que os pacientes ficam sem suporte respiratório. Neste artigo de protocolo, descrevem-se a fundamentação, o desenho e a situação do ensaio. Identificador do ClinicalTrials.gov:NCT04468087

ABSTRACT Repurposed drugs are important in resource-limited settings because the interventions are more rapidly available, have already been tested safely in other populations and are inexpensive. Repurposed drugs are an effective solution, especially for emerging diseases such as COVID-19. The REVOLUTIOn trial has the objective of evaluating three repurposed antiviral drugs, atazanavir, daclatasvir and sofosbuvir, already used for HIV- and hepatitis C virus-infected patients in a randomized, placebo-controlled, adaptive, multiarm, multistage study. The drugs will be tested simultaneously in a Phase II trial to first identify whether any of these drugs alone or in combination reduce the viral load. If they do, a Phase III trial will be initiated to investigate if these medications are capable of increasing the number of days free respiratory support. Participants must be hospitalized adults aged ≥ 18 years with initiation of symptoms ≤ 9 days and SpO2 ≤ 94% in room air or a need for supplemental oxygen to maintain an SpO2 > 94%. The expected total sample size ranges from 252 to 1,005 participants, depending on the number of stages that will be completed in the study. Hence, the protocol is described here in detail together with the statistical analysis plan. In conclusion, the REVOLUTIOn trial is designed to provide evidence on whether atazanavir, daclatasvir or sofosbuvir decrease the SARS-CoV-2 load in patients with COVID-19 and increase the number of days patients are free of respiratory support. In this protocol paper, we describe the rationale, design, and status of the trial. ClinicalTrials.gov identifier:NCT04468087

https://doi.org/10.5935/0103-507x.20220002-pt

The α-Tomatine Exhibits Antiproliferative Activity, Rupture of Cell Membranes and Induces the Expression of APC Gene in the Human Colorectal Adenocarcinoma Cell Line (Ht-29)
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Ishii, Priscila Lumi

; Oliveira, Rodrigo Juliano

; Mauro, Mariana de Oliveira

; Zorgetto-Pinheiro, Verônica Assalin

; Sartori, Daniele

; Lepri, Sandra Regina

; Baranoski, Adrivanio

; Mantovani, Mário Sérgio

; Ribeiro, Lúcia Regina

Brazilian Archives of Biology and Technology

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2020, Volume 63 elocation e20190395

Abstract: En | Text: En | PDF: En

Abstract The α-tomatine is a steroidal glycoalkaloid found in immature tomatoes (Lycopersicon esculentum) that has important biological functions including the inhibition of cancer cell growth and preventing metastasis. This study aimed to evaluate the effects of α-tomatine on cytotoxicity, cellular proliferation, apoptosis, and mRNA expression of APC, CCNA2, β-catenin, CASP9, BAK, BAX and BCL-XL in colorectal adenocarcinoma cell line HT-29. HT29 cells were treated with three concentrations of α-tomatine (0.1, 1 and 10 µg/mL), although only the 1 µg/mL concentration of α-tomatine was used to evaluate genetic expression patterns by real time-PCR. Results showed that α-tomatine was cytotoxic only at the 10 µg/mL concentration. Cell proliferation was significantly inhibited after the first 24 hours of treatment only with concentrations of 10 µg/mL. In contrast, there were no significant differences in apoptosis for any treatment. In the gene expression studies, only APC expression was significantly altered by α-tomatine treatment. In conclusion, α-tomatine has antiproliferative activity in the first 24h of treatment, does not induce apoptosis in this cell line and causes disruption of cell membranes, thereby increasing the expression of APC gene related to cell cycle.

https://doi.org/10.1590/1678-4324-2020190395 301 downloads

Atualização das Diretrizes em Cardiogeriatria da Sociedade Brasileira de Cardiologia – 2019
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Feitosa Filho, Gilson Soares

; Peixoto, José Maria

; Pinheiro, José Elias Soares

; Afiune Neto, Abrahão

; Albuquerque, Afonso Luiz Tavares de

; Cattani, Álvaro César

; Nussbacher, Amit

; Camarano, Ana Amelia

; Sichinels, Angela Hermínia

; Sousa, Antonio Carlos Sobral

; Alencar Filho, Aristóteles Comte de

; Gravina, Claudia F.

; Sobral Filho, Dario Celestino

; Pitthan, Eduardo

; Costa, Elisa Franco de Assis

; Duarte, Elizabeth da Rosa

; Freitas, Elizabete Viana de

; Moriguchi, Emilio Hideyuki

; Mesquita, Evandro Tinoco

; Fernandes, Fábio

; Fuchs, Felipe Costa

; Feitosa, Gilson Soares

; Pierre, Humberto

; Pereira Filho, Ilnei

; Helber, Izo

; Borges, Jairo Lins

; Garcia, Jéssica Myrian de Amorim

; Souza, José Antonio Gordillo de

; Zanon, José Carlos da Costa

; Alves, Josmar de Castro

; Mohallem, Kalil Lays

; Chaves, Laura Mariana de Siqueira Mendonça

; Moura, Lídia Ana Zytynski

; Silva, Márcia Cristina Amélia da

; Toledo, Maria Alice de Vilhena

; Assunção, Maria Elisa Lucena Sales de Melo

; Wajngarten, Mauricio

; Gonçalves, Mauro José Oliveira

; Lopes, Neuza Helena Moreira

; Rodrigues, Nezilour Lobato

; Toscano, Paulo Roberto Pereira

; Rousseff, Pedro

; Maia, Ricardo Antonio Rosado

; Franken, Roberto Alexandre

; Miranda, Roberto Dischinger

; Gamarski, Roberto

; Rosa, Ronaldo Fernandes

; Santos, Silvio Carlos de Moraes

; Galera, Siulmara Cristina

; Grespan, Stela Maris da Silva

; Silva, Teresa Cristina Rogerio da

; Esteves, William Antonio de Magalhães

Arquivos Brasileiros de Cardiologia

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may 2019, Volume 112 N. 5 Pages 649 - 705

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20190086 15622 downloads

Brown Flaxseed Prevents DNA Damage Induced by 1,2-Dimethylhydrazine in a Pre-Clinical Model
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Pamplona-Silva, Maria Tereza

; Morandi, Walter Vinicius

; Bernardi, Luana

; Tura, Bethânia Borges

; Oliveira, Diego Duarte Marques de

; Antoniolli-Silva, Andreia Conceição Milan Brochado

; Mauro, Mariana de Oliveira

; Oliveira, Rodrigo

Brazilian Archives of Biology and Technology

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2018, Volume 61 elocation e18180303

Abstract: En | Text: En | PDF: En

ABSTRACT The study evaluated the effects of brown flaxseed supplementation in natura on the prevention of DNA damage induced by 1,2-dimethylhydrazine (DMH) in vivo. The experimental groups were Negative and Positive Controls and the protocols of Pre-treatment, Simultaneous, Post-treatment, Pre+continuous in relation to the supplementation of brown flaxseed and administration with carcinogenic compound. The results showed that brown flaxseed supplementation does not cause genomic and genetic damage. In addition, brown flaxseed showed a chemopreventive food that reduced the damages assessed by the comet assay up to 94.07x and the damages assessed by the micronucleus assay up to 91.88x. Brown flaxseed supplementation also increased the frequency of monocytes and lymphocytes indicating immunological improvements. Thus, brown flaxseed supplementation is considered safe and reduces the frequency of DNA damage that can lead to tumors. Therefore, if these events are confirmed in humans, flaxseed will have reinforced its indication as a functional chemopreventive food in the prevention of cancer.

https://doi.org/10.1590/1678-4324-2018180303 2908 downloads

Cardanol: toxicogenetic assessment and its effects when combined with cyclophosphamide
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Schneider, Beatriz Ursinos Catelan

; Meza, Alisson

; Beatriz, Adilson

; Pesarini, João Renato

; Carvalho, Pamela Castilho de

; Mauro, Mariana de Oliveira

; Karaziack, Caroline Bilhar

; Cunha-Laura, Andréa Luiza

; Monreal, Antônio Carlos Duenhas

; Matuo, Renata

; Lima, Dênis Pires de

; Oliveira, Rodrigo Juliano

Genetics and Molecular Biology

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jun 2016, Volume 39 N. 2 Pages 279 - 289

Abstract: En | Text: En | PDF: En

Abstract Cardanol is an effective antioxidant and is a compound with antimutagenic and antitumoral activity. Here, we evaluated the genotoxic and mutagenic potential of saturated side chain cardanol and its effects in combination with cyclophosphamide in preventing DNA damage, apoptosis, and immunomodulation. Swiss mice were treated with cardanol (2.5, 5 and 10 mg/kg) alone or in combination with cyclophosphamide (100 mg/kg). The results showed that cardanol is an effective chemopreventive compound, with damage reduction percentages that ranged from 18.9 to 31.76% in the comet assay and from 45 to 97% in the micronucleus assay. Moreover, cardanol has the ability to reduce the frequency of apoptosis induced by cyclophosphamide. The compound did not show immunomodulatory activity. A final interpretation of the data showed that, despite its chemoprotective capacity, cardanol has a tendency to induce DNA damage. Hence, caution is needed if this compound is used as a chemopreventive agent. Also, this compound is likely not suitable as an adjuvant in chemotherapy treatments that use cyclophosphamide.

https://doi.org/10.1590/1678-4685-gmb-2015-0170 3271 downloads

Analysis of whey protein hydrolysates: peptide profile and ACE inhibitory activity
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Silvestre, Marialice Pinto Coelho

; Silva, Mauro Ramalho

; Silva, Viviane Dias Medeiros

; Souza, Mariana Wanessa Santana de

; Lopes Junior, Carlos de Oliveira

; Afonso, Wendel de Oliveira

Brazilian Journal of Pharmaceutical Sciences

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dec 2012, Volume 48 N. 4 Pages 747 - 757

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo deste estudo foi preparar hidrolisados enzimáticos do concentrado proteico do soro de leite com perfil peptídico nutricionalmente adequado e com capacidade para inibir a atividade da enzima conversora da angiotensina (ECA). Os efeitos do tipo de enzima usado (pancreatina ou papaína), da relação enzima:substrato (E:S=0,5:100, 1:100, 2:100 e 3:100) e do uso da ultrafiltração (UF) foram investigados. O fracionamento dos peptídeos foi feito por CLAE de exclusão molecular e a quantificação dos componentes das frações cromatográficas foi realizada pelo método da Área Corrigida da Fração. A atividade inibitória da ECA (AI-ECA) foi determinada por CLAE de fase reversa. Todos os parâmetros testados afetaram tanto o perfil peptídico quanto a AI-ECA. O melhor perfil peptídico foi atingido para os hidrolisados obtidos com papaína, enquanto a pancreatina foi mais vantajosa em termos da AI-ECA. O efeito benéfico do uso de menor relação E:S sobre o perfil peptídico e a AI-ECA foi observado para ambas as enzimas dependendo das condições usadas para o preparo dos hidrolisados. O efeito benéfico da ausência da UF sobre o perfil peptídico foi observado em alguns casos para pancreatina e papaína. No entanto, a ausência da UF produziu maior AI-ECA somente quando a papaína foi usada.

The aim of this study was to prepare enzymatic hydrolysates from whey protein concentrate with a nutritionally adequate peptide profile and the ability to inhibit angiotensin-converting enzyme (ACE) activity. The effects of the type of enzyme used (pancreatin or papain), the enzyme:substrate ratio (E:S ratio=0.5:100, 1:100, 2:100 and 3:100) and the use of ultrafiltration (UF) were investigated. The fractionation of peptides was performed by size-exclusion-HPLC, and the quantification of the components of the chromatographic fractions was carried out by a rapid Corrected Fraction Area method. The ACE inhibitory activity (ACE-IA) was determined by Reverse Phase-HPLC. All parameters tested affected both the peptide profile and the ACE-IA. The best peptide profile was achieved for the hydrolysates obtained with papain, whereas pancreatin was more advantageous in terms of ACE-IA. The beneficial effect of using a lower E:S ratio on the peptide profile and ACE-IA was observed for both enzymes depending on the conditions used to prepare the hydrolysates. The beneficial effect of not using UF on the peptide profile was observed in some cases for pancreatin and papain. However, the absence of UF yielded greater ACE-IA only when using papain.

9653 downloads

Ecophysiological and anatomical changes due to uptake and accumulation of heavy metal in Brachiaria decumbens
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Gomes, Marcelo Pedrosa

; Marques, Teresa Cristina Lara Lanza de Sá e Melo

; Nogueira, Mariana de Oliveira Gonçalves

; Castro, Evaristo Mauro de

; Soares, Ângela Maria

Scientia Agricola

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oct 2011, Volume 68 N. 5 Pages 566 - 573

Abstract: En | Text: En | PDF: En

The growth and developmental characteristics of grasses and their high biodiversity make such plants suitable for remediation of areas contaminated by heavy metals. Nevertheless, heavy metal toxic effect on the plants may cause alteration in their metabolic pathways, such as photosynthesis, respiration, and growth, modifying plant anatomy. This work aimed to evaluate the effect of levels of soil contamination (0, 7.5 % and 15 % m³ m-3) on biomass production, on photosynthetic characteristics and on anatomical changes in roots and leaves of brachiaria (Brachiaria decumbens Stapf.). After seeds were planted, seedlings were uprooted and replanted in vases containing soil at different contamination levels, being left to rest for 120 days. At the end of that time, plants presented reduced yield of root and shoot dry matter, contents of chlorophyll a, chlorophyll b, total chlorophyll and potential photosynthesis with increased of soil contamination. The cell layers of endodermis and exodermis in the root tissues and the cell walls of the xylem and cortical parenchyma all thickened as contamination increased. In the leaf tissues, the adaxial and abaxial epidermis presented increased thickness while the leaf blade presented reduced thickness as contamination increased with consequent change in the root growth rate. In general, the effects of heavy metal increased with the metal concentration. Some results indicate that B. decumbens seems to have some degree of heavy metal tolerance.

11679 downloads Cited 5 times in SciELO

Crise energética mundial e o papel do Brasil na problemática de biocombustíveis
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Pompelli, Marcelo Francisco

; Jarma Orozco, Alfredo de Jesús

; de Oliviera, Marciel Teixeira

; Monteiro Rodrigues, Bruno Rafael

; Barbosa, Mariana Oliveira

; Guida Santos, Mauro

; Morais de Oliveira, Antônio Fernando

; de Almeida-Cortez, Jarcilene Silva

; Fonseca-Moreno, Liz P

Agronomía Colombiana

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jun 2011, Volume 29 N. 2 Pages 423 - 433

Abstract: Pt | Text: Pt | PDF: Pt

O tema dos biocombustíveis gera grandes expectativas, tanto na população como na comunidade científica em geral. Os biocombustíveis têm notáveis efeitos na economia mundial, mas ainda quando relacionados à proteção dos ecossistemas, já que sua utilização pode promover a redução dos gases gerados pelo uso excessivo de compostos originários do petróleo, que produzem o efeito estufa. Neste cenário, países como o Brasil têm um papel fundamental, toda vez que podem liderar a produção mundial de biocombustíveis de origem vegetal. As principais espécies utilizadas como fonte de biocombustíveis no Brasil são a soja (Glycine max L.), e mais recentemente o pinhão-manso (Jatropha curcas L. e Jatropha molissima Pohl. ex. Baill). Estes fatos, aliados à grande disponibilidade de terras no Brasil, fazem dele um país pioneiro na produção de biocombustíveis, que, junto com outros na mesma linha, atualmente está enfrentando o problema. Com esse fim, está-se procurando estabelecer novas espécies e sistemas de cultivo para viabilizar o plantio comercial de espécies nativas com grande potencial biocombustível

2069 downloads

Efeitos dos rejeitos da indústria de zinco na anatomia e crescimento de plantas jovens de Salix humboldtiana Willd: (salgueiro)
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Gomes, Marcelo Pedrosa

; Marques, Teresa Cristina Lara Lanza de Sá e Melo

; Nogueira, Mariana de Oliveira Gonçalves

; Silva, Guilherme Henrique

; Castro, Evaristo Mauro de

; Soares, Ângela Maria

Hoehnea

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mar 2011, Volume 38 N. 1 Pages 135 - 142

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A poluição por metais pesados, gerada principalmente pelas atividades de mineração e industrial, exige estudos que possibilitem a recuperação de áreas contaminadas. Foram estudadas respostas de crescimento e características anatômicas de plantas jovens de salgueiro cultivadas em solo multicontaminado por metais pesados. As plantas foram cultivadas em três níveis de misturas de solo contaminado: 0%; 15% e 30% (v/v), durante 60 dias. No maior nível de contaminação foi observado menor produção de matéria seca das raízes e da parte aérea e menor crescimento das raízes. Nesta condição, as raízes apresentaram maior espessura da epiderme, exoderme e endoderme e menor número de elementos condutores de xilema. As folhas apresentaram modificações no número de elementos condutores de xilema, na densidade e diâmetro dos estômatos, e na espessura da epiderme, endoderme, colênquima e do mesofilo.

The heavy metal pollution caused mainly by mining and industrial activities requires studies for the recovery of contaminated areas. Thus, we studied growth and anatomical characteristics responses of young willow plants grown in heavy metal multicontaminated soil. Plants were grown in three mixture levels of contaminated soil: 0%, 15% and 30% (v/v) for 60 days. At the highest level of contamination was noted a less dry matter production of roots and shoots and reduced root growth. Accordingly, the roots exhibited epidermis, exodermis and endodermis thicker, and a smaller number of conducting elements of xylem. Leaves showed changes in the number of conducting elements of xylem diameter and density of stomata, and epidermis, endodermis, collenchyma and mesophyll cell thickness.

2516 downloads

10.

Chemopreventive activity of phenylalanine against damage mutagenic prompted by the acute administration of cyclophosphamide in pregnant and non-pregnant mice using the micronucleus test
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Mauro, Mariana de Oliveira

; Pesarini, João Renato

; Ishii, Priscila Lumi

; Silva, Ariane Fernanda da

; Oliveira, Rodrigo Juliano

Revista Brasileira de Farmacognosia

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jul 2010, Volume 20 N. 3 Pages 334 - 339

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A presente pesquisa teve por objetivo avaliar a capacidade quimiopreventiva da fenilalanina. Utilizou-se um lote de fêmeas prenhes e não prenhes divididas nos seguintes grupos experimentais: G1, PBS (0,1 mL/kg p.c.); G2, ciclofosfamida (35 mg/kg p.c.-i.p.); G3, fenilalanina (150 mg/kg p.c.-v.o.) e G4, fenilalanina (300 mg/kg p.c.-v.o.) e G5 (150 mg/kg p.c. de fenilalanina e 35 mg/kg de ciclofosfamida); G6, (300 mg/kg p.c. de fenilalanina e 35 mg/kg de ciclofosfamida). As coletas de sangue periférico foram realizadas em T0, antes da administração de qualquer substância teste e/ou veículos, e igualmente em T24 e T48, onde as coletas foram realizadas respectivamente 24 e 48 h após a administração da ciclofosfamida. Em uma análise geral verificou-se que, para o grupo de fêmeas não prenhes, a avaliação da antimutagenicidade demonstrou porcentagens de redução de danos de 57,24% e 31,64% para G5 e G6, respectivamente, em T24, e 29,32% e 24,13% para G5 e G6, respectivamente, em T48. Nos animais prenhes a antimutagenicidade de 24 h demonstrou eficiência quimiopreventiva apenas para a menor dose (G5) e as porcentagens de redução de danos foram de 43,25% em G5 e 18,47% em G6. No momento T48 as porcentagens de redução de danos foram de 44,67% e 37,76% para G5 e G6, respectivamente.

This study aimed to evaluate the quimiopreventive ability of phenylalanine. We used pregnant and non-pregnant female mice divided into the following groups: G1-PBS, (0.1 mL/kg b.w); G2, cyclophosphamide (35 mg/kg p.c.-i.p.); G3 and G4, phenylalanine (150 and 300 mg/kg b.w respectively-v.o.) and G5 and G6, association between the two doses of phenylalanine and cyclophosphamide, respectively. The peripheral blood samples were taken at T0, before the administration of any drug test and / or vehicles, also at T24 and T48 where the collections were made 24 and 48 h after administration of cyclophosphamide, respectively. A general analysis has found that, for the group of non-pregnant female, the antimutagenic evaluation showed reduction percentages of damage of 57.24% and 31.64% for G5 and G6, respectively, at T24, and 29.32% and 24.13% for G5 and G6, respectively, at T48. Antimutagenic pregnant animals in the 24 h quimiopreventive efficiency shown only for the lower dose (G5) and the percentages of reduction were 43.25% in G5 and G6 at 18.47%. At T48 the harm-reduction percentages were 44.67% and 37.76% for G5 and G6, respectively.

1964 downloads Cited 1 time in SciELO

11.

Avaliação da mutagenicidade e antimutagenicidade de um biopolímero extraído do microorganismo Agrobacterium radiobacter em camundongos Swiss
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Primo, Milka Selestina

; Calliari, Caroline Maria

; Castro-Gómez, Raúl Jorge Hernan

; Mauro, Mariana de Oliveira

; Mantovani, Mário Sérgio

; Oliveira, Rodrigo Juliano

Revista Brasileira de Farmacognosia

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jul 2010, Volume 20 N. 3 Pages 340 - 347

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A presente pesquisa avaliou a ação mutagênica e antimutagênica de um biopolímero de glucose extraído da Agrobacterium radiobacter (Biopolímero de Agrobacterium radiobacter). O experimento foi realizado com camundongos Swiss machos divididos em oito grupos. O tratamento com o biopolímero foi realizado por gavage em dose única concomitante a uma dose de solução tampão fosfato nos grupos de avaliação da mutagenicidade, ou ao agente indutor de danos no DNA, ciclofosfamida, na concentração de 50 mg/kg (peso corpóreo - p.c.), nos grupos de avaliação da antimutagenicidade. Utilizou-se o teste de micronúcleo em sangue periférico e a coleta de sangue foi realizada 24 e 48 h após a aplicação das substâncias-teste. A análise estatística demonstrou que o biopolímero não possui atividade mutagênica e que é efetivo em prevenir danos no DNA. As porcentagens de redução de danos nos grupos de antimutagenicidade foram de 83,9%, 89,1% e 103,1% em 24 h e 101,24%, 98,14% e 120,64% em 48 h para as doses de 75, 150 e 300mg/kg (p.c.), respectivamente. A alta porcentagem de redução de danos associada à ausência de efeitos mutagênicos indica, além da atividade quimioprotetora, a possibilidade do biopolímero ser um alimento funcional candidato à utilização como co-adjuvante na quimioterapia para prevenir efeitos colaterais.

This study evaluated the mutagenic and ant mutagenic action of a biopolymer of glucose extracted from Agrobacterium radiobacter (Biopolymer of Agrobacterium radiobacter). The experiment was conducted with Swiss male mice divided into eight groups. Treatment with the biopolymer was performed in a single dose by gavage at a dose of concomitant phosphate buffer groups in the evaluation of mutagenicity, or the agent of inducing DNA damage, cyclophosphamide, the concentration of 50 mg/kg (body weight --b.w.), in groups of assessment ant mutagenic. We used the micronucleus test in peripheral blood. The blood sample was held 24 and 48 h after application of the test substances. Statistical analysis showed that the biopolymer has no mutagenic activity and it is effective in preventing damage to DNA. The percentages of damage reduction in groups of ant mutagenic were 83.9%, 89.1% and 103.1% in 24 h and 101.24%, 98.14% and 120.64% at doses of 48 to 75, 150 and 300 mg/kg (b.w.) respectively. The high percentage of damage reduction associated with the absence of mutagenic effects indicates the possibility of biopolymer chemoprotection action. It can also be considered a functional food candidate to be used as co-adjuvant chemotherapy to prevent side effects.

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Glossary and search help

You can enrich your search in a very simple way. Use the search indexes combined with the connectors (AND or OR) and specify more your search.

For example, if you want to search for articles about
cases of dengue in Brasil in 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

See below the complete list of search indexes that can be used:

Index code	Element
ti	article title
au	author
kw	article keywords
subject	subject (title words, abstract and keywords)
ab	abstract
ta	journal short title (e.g. Cad. Saúde Pública)
journal_title	journal full title (e.g. Cadernos de Saúde Pública)
la	publication language code (e.g. pt - Portuguese, es - Spanish)
type	document type
pid	publication identifier
publication_year	publication year of publication
sponsor	sponsor
aff_country	country code of the author's affiliation
aff_institution	author affiliation institution
volume	article volume
issue	article issue
elocation	elocation
doi	DOI number
issn	journal ISSN
in	SciELO colection code (e.g. scl - Brasil, col - Colômbia)
use_license	article usage license code

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