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[SciELO Preprints] - Guidelines on the Diagnosis and Treatment of Hypertrophic Cardiomyopathy – 2024
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Fernandes, Fabio Simões, Marcus V. Correia, Edileide de Barros Marcondes-Braga, Fabiana G. Coelho-Filho, Otavio Rizzi Mesquita, Cláudio Tinoco Mathias-Junior, Wilson Rochitte, Carlos Eduardo Ramires, Felix José Alvarez Alves, Silvia Marinho Martins Montera, Marcelo Westerlund Lopes, Renato Delascio Oliveira-Junior, Mucio Tavares Scolari, Fernando L. Avila, Walkiria Samuel Canesin, Manoel Fernandes Bacal, Fernando Bocchi, Edimar Alcides Moura, Lídia Ana Zytynski Saad, Eduardo Benchimol Scanavacca, Mauricio I. Valdigem, Bruno Pereira Cano , Manuel Nicolas Abizaid , Alexandre Ribeiro, Henrique Barbosa Lemos-Neto, Pedro Alves Ribeiro, Gustavo Calado de Aguiar Jatene, Fabio Biscegli Dias, Ricardo Ribeiro Beck-da-Silva, Luis Rohde, Luis Eduardo P. Bittencourt, Marcelo Imbroinise Pereira, Alexandre Krieger, José Eduardo Villacorta, Humberto Martins, Wolney de Andrade Figueiredo-Neto, José Albuquerque de Cardoso , Juliano Novaes Pastore, Carlos Alberto Jatene, Ieda Biscegli Tanaka, Ana Cristina Sayuri Hotta, Viviane Tiemi Romano, Minna Moreira Dias Albuquerque, Denilson Campos de Mourilhe-Rocha, Ricardo Hajjar, Ludhmila Abrahão Brito, Fabio Sandoli de Caramelli , Bruno Calderaro, Daniela Farsky, Pedro Silvio Colafranceschi , Alexandre Siciliano Pinto, Ibraim Masciarelli Vieira , Marcelo Luiz Campos Danzmann, Luiz Claudio Barberato , Silvio Henrique Mady, Charles Martinelli-Filho, Martino Torbey , Ana Flavia Malheiros Schwartzmann, Pedro Vellosa Macedo, Ariane Vieira Scarlatelli Ferreira , Silvia Moreira Ayub Schmidt, Andre Melo , Marcelo Dantas Tavares de Lima-Filho, Moysés Oliveira Sposito, Andrei C. Brito, Flavio de Souza Biolo, Andreia Madrini-Junior, Vagner Rizk, Stéphanie Itala Mesquita, Evandro Tinoco

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Publication date: 2024-04-08

Abstract: Pt Es En

A cardiomiopatia hipertrófica (CMH) é uma forma de doença do músculo cardíaco de causa genética, caracterizada pela hipertrofia das paredes ventriculares. O diagnóstico requer detecção por métodos de imagem (Ecocardiograma ou Ressonância Magnética Cardíaca) de qualquer segmento da parede do ventrículo esquerdo com espessura > 15 mm, sem outra causa provável. A análise genética permite identificar mutações de genes codificantes de diferentes estruturas do sarcômero responsáveis pelo desenvolvimento da CMH em cerca de 60% dos casos, permitindo o rastreio de familiares e aconselhamento genético, como parte importante do manejo dos pacientes e familiares. Vários conceitos sobre a CMH foram recentemente revistos, incluindo sua prevalência de 1 em 250 indivíduos, não sendo, portanto, uma doença rara, mas subdiagnosticada. A vasta maioria dos pacientes é assintomática. Naqueles sintomáticos, a obstrução do trato de saída do ventrículo esquerdo (OTSVE) é o principal distúrbio responsável pelos sintomas, devendo-se investigar a sua presença em todos os casos. Naqueles em que o ecocardiograma em repouso ou com Manobra de Valsalva não detecta gradiente intraventricular significativo (> 30 mmHg), devem ser submetidos à ecocardiografia com esforço físico para detecção da OTSVE.   Pacientes com sintomas limitantes e grave OTSVE, refratários ao uso de betabloqueadores e verapamil, devem receber terapias de redução septal ou uso de novas drogas inibidoras da miosina cardíaca. Por fim, os pacientes adequadamente identificados com risco aumentado de morta súbita podem receber medida profilática com implante de cardiodesfibrilador implantável (CDI).

La miocardiopatía hipertrófica (MCH) es una forma de enfermedad cardíaca de origen genético, caracterizada por el engrosamiento de las paredes ventriculares. El diagnóstico requiere la detección mediante métodos de imagen (Ecocardiograma o Resonancia Magnética Cardíaca) que muestren algún segmento de la pared ventricular izquierda con un grosor > 15 mm, sin otra causa probable. El análisis genético permite identificar mutaciones en genes que codifican diferentes estructuras del sarcómero responsables del desarrollo de la MCH en aproximadamente el 60% de los casos, lo que permite el tamizaje de familiares y el asesoramiento genético, como parte importante del manejo de pacientes y familiares. Varios conceptos sobre la MCH han sido revisados recientemente, incluida su prevalencia de 1 entre 250 individuos, por lo tanto, no es una enfermedad rara, sino subdiagnosticada. La gran mayoría de los pacientes son asintomáticos. En los casos sintomáticos, la obstrucción del tracto de salida ventricular izquierdo (TSVI) es el trastorno principal responsable de los síntomas, y su presencia debe investigarse en todos los casos. En aquellos en los que el ecocardiograma en reposo o la maniobra de Valsalva no detecta un gradiente intraventricular significativo (> 30 mmHg), deben someterse a ecocardiografía de esfuerzo para detectar la obstrucción del TSVI. Los pacientes con síntomas limitantes y obstrucción grave del TSVI, refractarios al uso de betabloqueantes y verapamilo, deben recibir terapias de reducción septal o usar nuevos medicamentos inhibidores de la miosina cardíaca. Finalmente, los pacientes adecuadamente identificados con un riesgo aumentado de muerte súbita pueden recibir medidas profilácticas con el implante de un cardioversor-desfibrilador implantable (CDI).

Hypertrophic cardiomyopathy (HCM) is a form of genetically caused heart muscle disease, characterized by the thickening of the ventricular walls. Diagnosis requires detection through imaging methods (Echocardiogram or Cardiac Magnetic Resonance) showing any segment of the left ventricular wall with a thickness > 15 mm, without any other probable cause. Genetic analysis allows the identification of mutations in genes encoding different structures of the sarcomere responsible for the development of HCM in about 60% of cases, enabling screening of family members and genetic counseling, as an important part of patient and family management. Several concepts about HCM have recently been reviewed, including its prevalence of 1 in 250 individuals, hence not a rare but rather underdiagnosed disease. The vast majority of patients are asymptomatic. In symptomatic cases, obstruction of the left ventricular outflow tract (LVOT) is the primary disorder responsible for symptoms, and its presence should be investigated in all cases. In those where resting echocardiogram or Valsalva maneuver does not detect significant intraventricular gradient (> 30 mmHg), they should undergo stress echocardiography to detect LVOT obstruction. Patients with limiting symptoms and severe LVOT obstruction, refractory to beta-blockers and verapamil, should receive septal reduction therapies or use new drugs inhibiting cardiac myosin. Finally, appropriately identified patients at increased risk of sudden death may receive prophylactic measure with implantable cardioverter-defibrillator (ICD) implantation.

https://doi.org/10.1590/SciELOPreprints.8394

Aspectos morfológicos del absceso epidural espinal piógeno
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Bazán, Pedro L.

; Godoy Adaro, Alfredo O.

; Padini, Emmanuel

; Casco, Enrique A.

; Maruñak, Mathias

Revista de la Asociación Argentina de Ortopedia y Traumatología

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2023, Volume 88 N. 2 Pages 148 - 155

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Introduction: A pyogenic spinal epidural abscess is an infectious collection inside the spinal canal, outside the dural sac. The diagnosis is difficult and the consequences are devastating due to compression or vascular compromise. MRI has a high diagnostic sensitivity and specificity, which improves when a contrast medium is used. Objectives: To determine the inter- and intra-observer reproducibility, and to discriminate the different parameters and differences between specialties. Materials and Methods: Twenty-seven independent observers evaluated 5 parameters: region, location, involvement, association, and perivertebral, anterior, lateral, or posterior extravertebral abscess. The kappa coefficient was used to analyze 35 cases on three occasions. Results: The overall intra- and inter-observer global agreement level is kappa 0.76, with the following values obtained: region 0.94; location 0.88; involvement 0.55; association 0.67 and perivertebral abscess 0.77. The first three parameters indicate volume, while the final two indicate the presence of vertebral infectious foci outside the canal. Conclusions: The proposed morphological classification is simple to use and has high intra- and inter-observer reproducibility. The most reproducible parameters are region and location (>0.87).

Resumen Introducción: El absceso epidural espinal piógeno es la localización de una colección infecciosa dentro del canal raquídeo, por fuera del saco dural. El diagnóstico es difícil y las consecuencias son devastadoras a causa de la compresión o el compromiso vascular. La resonancia magnética tiene una alta sensibilidad y especificidad diagnósticas que aumentan si se administra un medio de contraste. Los objetivos de este estudio fueron: determinar la reproducibilidad inter- e intraobservador, y discriminar los distintos parámetros y diferencias entre especialidades. Materiales y Métodos: Veintisiete observadores independientes evaluaron 5 parámetros: región, ubicación, compromiso, asociación y perivertebral, absceso extravertebral anterior, lateral o posterior. Se analizaron 35 casos en tres oportunidades, se utilizó el coeficiente kappa. Resultados: El nivel de acuerdo global intra- e interobservador global fue kappa 0,76; los valores obtenidos fueron: región 0,94; ubicación 0,88; compromiso 0,55; asociación 0,67 y perivertebral 0,77. Los tres primeros parámetros dan noción de volumen y los dos últimos reflejan la presencia de focos infecciosos vertebrales por fuera del conducto. Conclusiones: La clasificación morfológica propuesta es de uso simple y tiene una muy buena tasa de reproducibilidad intra- e interobservador. Los parámetros con mayor reproducibilidad son: región y ubicación (>0,87).

https://doi.org/10.15417/issn.1852-7434.2023.88.2.1513

Focal spinal hyperesthesia as a prognostic factor in paraplegic dogs without deep pain perception
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Wrzesinski, Mathias. R.

; Rippinger, Angel

; Schwab, Marcelo. L.

; Ferrarin, Denis. A.

; Rauber, Júlia. S.

; Beckmann, Diego. V.

; Schamall, Ragnar F.

; Mazzanti, Alexandre

Pesquisa Veterinária Brasileira

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2022, Volume 42 elocation e06929

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A extrusão do disco intervertebral (EDIV) é a causa mais comum de lesão compressiva na medula espinhal de cães, cujo prognóstico é variável e depende de diversos fatores, sendo a percepção de dor profunda (PDP) o principal parâmetro utilizado. Pesquisas de novos fatores prognósticos são estudados com intuito de auxiliar na estimativa mais precisa de recuperação funcional. Com isso, o objetivo do estudo foi avaliar se a hiperestesia espinhal (HE) no local da compressão, pode ser utilizada como um fator prognóstico para recuperação funcional de cães com extrusão aguda do disco intervertebral (Hansen tipo I), sem a presença de PDP submetidos a hemilaminectomia toracolombar. A cirurgia descompressiva ocorreu no mesmo dia ou no dia seguinte ao atendimento. A duração da perda de dor profunda até a realização da cirurgia variou de 1 a 60 dias, com uma mediana de 4,5 dias para o grupo de cães com e 5,5 dias para aqueles sem hiperestesia espinhal. Dos 68 cães incluídos nesse estudo retrospectivo, 73,5% (50/68) apresentavam HE e, em 26,5% (18/68) a dor não foi identificada. A recuperação foi satisfatória nos cães com HE em 60% (30/50) e, sem HE, em 27,7% (5/18) dos casos, demonstrando que os cães paraplégicos sem PDP, mas com presença de hiperestesia espinhal tem 3,9 vezes mais chances de recuperação quando comparado com cães na mesma condição, mas sem HE. Não foram encontrados trabalhos que avaliaram a HE mediante a palpação da coluna vertebral como um fator prognóstico, o que reforça a relevância do presente estudo. Os resultados do presente trabalho sugerem que a HE em cães paraplégicos acometidos por EDIV toracolombar sem presença de PDP pode ser utilizada como um possível indicador prognóstico de recuperação funcional.

ABSTRACT: Intervertebral disc extrusion (IVDE) is the most common cause of spinal cord compression in dogs, whose prognosis is variable and depends on several factors, with deep pain perception (DPP) being the main parameter used. Investigations of new prognostic factors are studied to assist in the estimation of functional recovery. Thus, this study aimed to evaluate whether spinal hyperesthesia (SH) at the compression site can be used as a prognostic factor for the functional recovery of dogs with acute IVDE (Hansen type I), without DPP being subjected to thoracolumbar hemilaminectomy. Decompression surgery was performed on the same day or the day after admission. The duration of the loss of DPP until surgery performance ranged from 1 to 60 days, with a median of 4.5 days for the group of dogs with SH and 5.5 days for those without SH. Among the 68 dogs included in this retrospective study, 73.5% (50/68) showed SH, and 26.5% (18/68) were not identified. Recovery was satisfactory in 60% (30/50) of dogs with SH and in 27.7% (5/18) of dogs without SH, demonstrating that paraplegic dogs without DPP but with SH were 3.9 times more likely to recover when compared to dogs in the same condition, but with no SH. No studies have evaluated SH by palpation of the spine as a prognostic factor, which reinforces the relevance of the present study. The results of this study imply that SH in paraplegic dogs affected by thoracolumbar IVDE, without the presence of DPP, can be used as a possible prognostic indicator of functional recovery.

https://doi.org/10.1590/1678-5150-pvb-6929

Complications after cerebrospinal fluid collection in dogs with brain neoplasm
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Wrzesinski, Mathias R.

; Ripplinger, Angel

; Ferrarin, Dênis A.

; Schwab, Marcelo L.

; Rauber, Júlia S.

; Santos, Junior

; Beckmann, Diego V.

; Mazzanti, Alexandre

Pesquisa Veterinária Brasileira

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2022, Volume 42 elocation e06984

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A colheita do líquido cerebroespinhal (LCE) em cães com neoplasmas encefálicos (NE) pode estar associada a complicações devido ao aumento da pressão intracraniana (PIC), causada pela lesão expansiva. Embora o procedimento seja realizado em cães com NE, não foram encontrados dados referentes às complicações após colheita de LCE nesses pacientes. Sendo assim, o objetivo do presente estudo retrospectivo foi identificar a taxa, bem como os tipos de complicações observadas após a colheita de LCE em cães com neoplasma encefálico. Dos 30 cães com NE incluídos no estudo, em 83% (25/30) dos casos a evolução clínica após a colheita do LCE foi considerada normal e, em 17% (5/30) anormal. Os principais sinais clínicos e neurológicos observados nos cães com evolução clínica anormal foram apneia (5/5), reflexo fotomotor pupilar ausente (3/5), coma (2/5) e estupor (1/5). Em 40% (2/5) foi observado herniação do cerebelo na necropsia. Com base nos resultados encontrados, conclui-se que a taxa de complicações após a colheita de LCE foi baixa (17%) e as alterações encontradas foram apneia, seguido de reflexo fotomotor pupilar ausente, alteração no nível de consciência e herniação encefálica.

ABSTRACT: Cerebrospinal fluid (CSF) collection in dogs with brain neoplasms (BN) may be associated with complications owing to increased intracranial pressure caused by expansive lesions. Although this procedure has been performed in dogs with BN, no data regarding complications after CSF tap in these animals is available. Thus, this retrospective study aimed to identify the rate and types of complications observed after CSF taps in dogs with BN. Thirty dogs with BN were included in the study. In 83% (25/30) of the cases, clinical recovery after CSF tap was considered normal, and in 17% (5/30) the recovery was abnormal. The main clinical and neurological signs observed in dogs with abnormal clinical recovery were apnea (5/5), absence of pupillary photomotor reflex (3/5), coma (2/5), and stupor (1/5). In 40% (2/5) of the dogs, herniation of the cerebellum through the foramen magnum was observed on necropsy. In conclusion, the rate of complications after CSF taps was 17%, and was characterized by apnea, absent pupillary photomotor reflex, altered level of consciousness, and encephalic herniation.

https://doi.org/10.1590/1678-5150-pvb-6984

Association between SNP rs527616 in lncRNA AQP4-AS1 and susceptibility to breast cancer in a southern Brazilian population
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Marchi, Rafael D.

; Mathias, Carolina

; Reiter, Gabriel A. K.

; Lima, Rubens Silveira de

; Kuroda, Flávia

; Urban, Cícero de Andrade

; Souza, Ricardo. L. R. de

; Gradia, Daniela F.

; Ribeiro, Enilze M. S. F.

; Cavalli, Iglenir J.

; Oliveira, Jaqueline Carvalho de

Genetics and Molecular Biology

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2021, Volume 44 N. 1 elocation e20200216

Abstract: En | Text: En | PDF: En

Abstract Breast cancer (BC) is the leading cause of death by this disease in women worldwide. Among the factors involved in tumorigenesis, long non-coding RNAs (lncRNAs) and their differential expression have been associated. Differences in gene expression may be triggered by variations in DNA sequence, including single nucleotide polymorphisms (SNPs). In the present study, we analyzed the rs527616 (C>G), located in the lncRNA AQP4-AS1, using PCR-SSP in 306 BC patients and 312 controls, from a Brazilian population. In the BC group, the frequency found for CG heterozygotes was above the expected and the overdominant model is the best one to explain our results (OR: 1.70, IC 95%: 1.23-2.34, P<0.001). Furthermore, the SNP were associated with age at BC diagnosis and the risk genotype more frequent in the older age group. According to TCGA data, AQP4-AS1 is down-regulated in BC tissue, and the overexpression is associated with better prognoses, including Luminal A, HER2-, stage 1 of disease and smaller tumor. In conclusion, the CG genotype is associated with increased susceptibility in the southern Brazilian population. This SNP is mapped in the lncRNA AQP4-AS1, showing differential expression in BC samples. Based on these results, we emphasize the potential of the role of AQP4-AS1 in cancer.

https://doi.org/10.1590/1678-4685-gmb-2020-0216 788 downloads

The need for opioid in the postoperative analgesia of dogs undergoing hemilaminectomy due to intervertebral disc extrusion
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Ferrarin, Dênis A.

; Schwab, Marcelo L.

; Wrzesinski, Mathias R.

; Ripplinger, Angel

; Rauber, Júlia S.

; Santos, Anne G.C.

; Aiello, Graciane

; Mazzanti, Alexandre

Pesquisa Veterinária Brasileira

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2021, Volume 41 elocation e06825

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo deste estudo foi avaliar a analgesia pós-operatória de protocolos com e sem o opioide metadona em cães com extrusão de disco intervertebral submetidos à descompressão cirúrgica. Dezesseis cães paraplégicos com presença de nocicepção foram submetidos à hemilaminectomia/fenestração de disco e distribuídos aleatoriamente em dois grupos. No Grupo I (G1), o protocolo analgésico consistiu em metadona, meloxicam e dipirona e, no Grupo II (G2), por meloxicam e dipirona. Os pacientes foram avaliados de maneira cega por dois avaliadores, com base na escala visual analógica (EVA) e na escala simplificada composta de dor de Glasgow (CMPS-SF). As avaliações ocorreram a cada 2 horas durante as primeiras 24 horas de pós-operatório e, por mais 24 horas, a cada 4 horas. Não houve diferença estatística entre os grupos avaliados em relação à escores de dor e nem a necessidade de resgate analgésico. Ambos os protocolos promoveram analgesia nas 48 horas iniciais de pós-operatório, demonstrando não haver a necessidade do uso de opioide em cães submetidos à hemilaminectomia e fenestração de disco.

ABSTRACT: The aim of this study was to evaluate the postoperative analgesic effect of protocols with and without the opioid methadone in dogs with intervertebral disc extrusion undergoing decompressive surgery. Sixteen paraplegic dogs with preserved nociception underwent hemilaminectomy/disc fenestration and were randomly assigned to two groups. The analgesic protocol consisted of methadone, meloxicam and dipyrone in Group I (G1), and meloxicam and dipyrone in Group II (G2). The animals were blindly assessed by two observers, using the visual analogue scale (VAS) and the short-form Glasgow Composite Measure Pain Scale (CMPS-SF). Assessments occurred every 2 hours during first 24 hours post-surgery, and every 4 hours afterwards. There was no statistical difference among groups regarding pain scores or analgesic rescues. Both analgesic protocols provided analgesia in the initial 48 hours postoperatively, demonstrating that opioids are not necessary in the postoperative period of dogs undergoing hemilaminectomy and disc fenestration.

https://doi.org/10.1590/1678-5150-pvb-6825 135 downloads

Insecticidal potential of physic nut fruits of different stages of maturation on Myzus persicae (Hemiptera: Aphididae)
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Holtz, Anderson Mathias

; Marinho-Prado, Jeanne Scardini

; Cofler, Tatiane Pereira

; Mamedes Piffer, Ana Beatriz

; Gomes, Mylena da Silva

; Borghi Neto, Vergilio

Idesia (Arica)

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2021, Volume 39 N. 1 Pages 93 - 99

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Jatropha curcas L. (Euphorbiaceae) is a plant species widely present in tropical and subtropical regions that have been studied mainly for its potential for biofuel production; it is also a plant known for its toxic substances. To explore its use as an insecticide, we studied the effect of the seed extract from green fruit and the seed oils from the mature and dried fruit of J. curcas on the green aphid Myzus persicae (Hemiptera: Aphididae). Seven concentrations of extracts and oils of different maturation stages of fruits were used and sprayed with the Potter Spray Tower on cabbage leaves containing 10 individuals of M. persicae. Ten repetitions for each concentration were carried out at each stage of maturation (green, ripe, and dry), and the evaluation of the mortality of individuals occurred for a period of 72 hours. The maturation stage of J. curcas, as well as the concentration and time after the application, influenced the mortality of M. persicae. The best results were obtained 48h after applying the dried fruit oil at 2.5% and 72 hours after the application of dry and ripe fruit oil at 2.5%.

RESUMEN La Jatropha curcas L. (Euphorbiaceae) es una especie vegetal, ampliamente presente en regiones tropicales y subtropicales, que ha sido estudiada principalmente por su potencial para la producción de biocombustibles. Pero también es una planta conocida por sus sustancias tóxicas. Para verificar su uso como insecticida, estudiamos el efecto del extracto de semillas de frutos verdes y aceites de semillas de frutos maduros y secos de J. curcas sobre el pulgón verde Myzus persicae (Hemiptera: Aphididae). Siete soluciones, en diferentes concentraciones de los extractos y aceites, fueron utilizadas y pulverizadas con la Torre de Pulverización de Potter en hojas de repollo, con 10 individuos de M. persicae cada una. Se realizaron diez repeticiones para cada concentración en cada etapa de maduración (verde, maduro y seco) y la evaluación de la mortalidad de los individuos ocurrió en un período de 72 horas. El estadio de maduración de J. curcas, la concentración y el tiempo después de la aplicación influyeron la mortalidad de M. persicae. Los mejores resultados se obtuvieron 48 horas después de la aplicación del aceite de frutos secos a 2,5%, y 72 horas después del aceite de los frutos secos y maduros a 2,5%.

159 downloads

Analysis of bioactive compounds in umbu (Spondias tuberosa) by application of edible coating based on Chlorella sp during storage
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TEODOSIO, Albert Einstein Mathias de Medeiros

; ARAÚJO, Railene Hérica Carlos Rocha

; SANTOS, Bárbara Genilze Figueiredo Lima

; LINNÉ, Jéssica Aline

; SILVA, Katia Gomes da

; GOMES, Fernando Antônio Lima

; SOUZA, Gleyse Lopes Fernandes de

; LIMA, José Franciraldo de

Food Science and Technology

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sep 2020, Volume 40 N. 3 Pages 756 - 760

Abstract: En | Text: En | PDF: En

Abstract The use of edible coating has been an alternative to maintain the quality of vegetables and prolong their post-harvest life. This study aims to evaluate bioactive compounds in Spondias tuberosa submitted to different coating formulations based on Chlorella sp. associated with the pomegranate seed oil, to establish the one that best maintains post-harvest quality. The experiment was set in a completely randomized design, in a 5 × 6 factorial scheme, represented by five coating techniques (T1 = control; T2 = 0.5% Chlorella sp.; T3 = 1% Chlorella sp.; T4 = 1.5% Chlorella sp.; T5 = 2% Chlorella sp.), using 0.3% of pomegranate seed oil in coatings, during five evaluation periods (0, 1, 2, 3, 4 and 5 days), stored in BOD incubator at 24 ± 2 °C and 85 ± 5% RH, with three replicates of ten fruits per plot. Chlorella sp. associated with pomegranate seed oil produced a modified atmosphere around the fruit, retarding the ripening process. Coatings preserved the content of ascorbic acid and phenolic compounds. The treatment containing 2.0% of Chlorella sp. associated with pomegranate seed oil provided the best maintenance for fruit quality.

https://doi.org/10.1590/fst.19219 447 downloads

Agar and pomegranate seed oil used in a biodegradable coating composition for Formosa papaya
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MORAIS, Fabricio Alves de

; ARAÚJO, Railene Hérica Carlos Rocha

; OLIVEIRA, Agda Malany Forte de

; ALVES, Kalinny de Araújo

; VITOR, Raissa Cristina Leandro

; MORAIS, Sinthya Kelly Queiroz

; TEODOSIO, Albert Einstein Mathias Medeiros

; ONIAS, Elny Alves

; OLIVEIRA, Airton Gonçalves de

Food Science and Technology

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jun 2020, Volume 40 Pages 280 - 286

Abstract: En | Text: En | PDF: En

Abstract The use of biodegradable coatings to conserve fruits and vegetables stands out in the food industry. This study aims to evaluate the postharvest quality of papaya Formosa 'Tainung 01' submitted to different coating formulations, to establish the one that best maintains the postharvest quality. The fruits, distributed in a completely randomized design, received the coatings composed of aqueous solution of agar and pomegranate seed oil in the following compositions (with 6 treatments and 5 replicates): T1: 0.5% agar and 0.1 mL/L oil; T2: 1.0% agar and 0.2 mL/L oil; T3: 1.5% agar and 0.3 mL/L oil; T4: 2.0% agar and 0.4 mL/L of oil; T5: 2.5% of agar and 0.5 mL/L of oil; and T6: Control, uncoated. The experiment lasted ten days packing at 17.5 ± 0.6 °C and 55 ± 3, 2% RH. T4 provided better conservation of the quality attributes in papaya. Fruits in this treatment showed higher vitamin C content, higher soluble solids and total sugars, and lower titratable acidity, important characteristics for product acceptance. Besides, T4 better conserved the green (external) color of the fruits, while fruits submitted to the other treatments acquired a yellowish coloration.

https://doi.org/10.1590/fst.10119 767 downloads

10.

Clinical management of dogs with presumptive diagnosis of cervical intervertebral disc disease: 78 cases (2006-2017)
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Baumhardt, Raquel

; Ripplinger, Angel

; Aiello, Graciane

; Schwab, Marcelo L.

; Ferrarin, Denis A.

; Wrzesinski, Mathias R.

; Moro, Samanta S.

; Mazzanti, Alexandre

Pesquisa Veterinária Brasileira

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mar 2020, Volume 40 N. 3 Pages 176 - 180

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo desse estudo foi identificar cães com diagnóstico presuntivo de doença do disco intervertebral cervical (DDIV; C1-C5 ou C6-T2) submetidos ao tratamento clínico e avaliar a resposta a terapia instituída e o índice de recidiva. Esse estudo também visou demonstrar a idade, o gênero e a resposta ao tratamento de acordo com o grau neurológico, a fim de verificar se esses parâmetros podem ser utilizados como fatores prognósticos para a evolução clínica desses pacientes. Foram revisados os registros neurológicos do Hospital Veterinário Universitário de janeiro de 2006 a março de 2017. Realizaram coleta de dados a partir dos registros e por meio de um questionário respondido pelos tutores. Avaliaram 177 fichas neurológicas de cães e obtidas informações para inclusão no estudo em 78 delas. As raças mais frequentes foram Dachshunds, seguido dos cães sem raça definida. Quanto ao grau de disfunção neurológica, 58,97% apresentavam grau I (somente dor), 25,64% estavam em grau II (tetraparesia ambulatória) e 15,38% em grau III (tetraparesia não ambulatória). O repouso absoluto e em espaço restrito foram realizados em 75,64% e 24,36% dos casos, respectivamente e com duração de no mínimo uma semana, podendo chegar a mais de quatro semanas. A maioria dos animais era de pequeno porte (≤15kg). A recuperação foi satisfatória em 87,17% dos cães e insatisfatória em 12,83%. Quanto à recidiva, esta foi observada em 10,3% dos pacientes com recuperação satisfatória. O tratamento clínico para cães com DDIV cervical pode ser indicado com adequada resposta clínica para graus de disfunção que variam de I a III, seja em repouso absoluto ou em espaço restrito e com baixo índice de recidiva.

ABSTRACT: This study aimed to identify dogs with a presumptive diagnosis of cervical intervertebral disc disease (IVDD; C1-C5 or C6-T2) submitted to clinical management and evaluate the outcome. This study also aimed to demonstrate the age, sex, and treatment response according to the neurological degree, and verify whether those factors could potentially influence the outcome. The data were obtained from patients with a neurological dysfunction, admitted at the Veterinary Hospital from January 2006 to March 2017. In addition to patient records, the tutors answered a questionnaire related to the success of therapy. A hundred and seventy-seven neurological records were evaluated, and 78 were included in the study according to the inclusion criteria. The most frequent breeds were Dachshunds, followed by mixed-breed dogs. Regarding the neurological dysfunction degree, 58.97% presented grade I (only neck pain), 25.64% were grade II (ambulatory tetraparesis), and 15.38% grade III (nonambulatory tetraparesis). Absolute and partial space rest were performed in 75.64% and 24.36% of the cases, respectively. The minimum rest time was one week and could come up to four weeks. Most dogs were small-sized (≤15kg). The recovery was satisfactory in 87.17% of dogs and unsatisfactory in 12.83%. Regarding recurrence, we observed that 10.3% of dogs presented satisfactory recovery. The clinical treatment for dogs with cervical IVDD can be indicated with adequate clinical response to dysfunction degrees ranging from I to III, either at rest or in restricted space and with a low rate of relapse.

https://doi.org/10.1590/1678-5150-pvb-6049 863 downloads

11.

Clinical management of dogs with presumptive diagnosis of thoracolumbar intervertebral disc disease: 164 cases (2006-2017)
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Baumhardt, Raquel

; Ripplinger, Angel

; Aiello, Graciane

; Schwab, Marcelo L.

; Ferrarin, Denis A.

; Wrzesinski, Mathias R.

; Rauber, Julia

; Mazzanti, Alexandre

Pesquisa Veterinária Brasileira

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jan 2020, Volume 40 N. 1 Pages 55 - 60

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo desse estudo foi identificar cães com diagnóstico presuntivo de DDIV toracolombar submetidos ao tratamento clínico, a fim de avaliar a resposta à terapia instituída. Foram revisados os registros neurológicos de cães atendidos pelo Serviço de Neurologia e Neurocirurgia Veterinária no período de 2006 a 2017 de um Hospital Veterinário Universitário. Foi realizada coleta de dados a partir dos registros e por meio de um questionário respondido pelos tutores. Foram avaliadas 413 fichas neurológicas de cães e obtidas informações para inclusão no estudo em 164 delas. As raças mais frequentes foram dachshunds, seguido de cães sem raça definida. Quanto ao grau de disfunção neurológica foi definido como grau I para 15,9% dos cães, grau II para 25,6%, grau III para 26,8%, grau IV para 8,5% e grau V para 23,2%. A recuperação foi satisfatória em 71,6% dos cães e insatisfatória em 28,4%. Dos que se recuperaram satisfatoriamente, 27,7% tiveram recidivas. Com base nos resultados obtidos pode-se concluir que o tratamento clínico em repouso absoluto e administração de anti-inflamatórios e analgésicos opióides para cães com DDIV toracolombar é efetivo, principalmente para cães em graus mais leves da doença (grau I, II e III). Há possibilidade de recidiva com esse tipo de terapia cujos sinais clínicos poderão ser mais graves.

ABSTRACT: This study aimed to identify dogs with presumptive diagnosis of cervical intervertebral disc disease (IVDD) submitted to clinical management and to evaluate the outcomes. Data were obtained from the medical records of patients with neurological dysfunction assisted at a University Veterinary Hospital from 2006 to 2017. In addition to the patients’ records, dog owners responded to a questionnaire on the success of therapy. Four hundred and thirteen neurological records were evaluated, and 164 met the inclusion criteria of the study. The most common breed was Dachshund, followed by mongrels. Classification of neurological dysfunction in the study sample was as follows: 15.9% with grade I, 25.6% with grade II, 26.8% with grade III, 8.5% with grade IV, and 23.2% with grade V. Outcome was satisfactory in 71.6% of the dogs and unsatisfactory in 28.4% of them. Recurrence was observed in 27.7% of those with satisfactory outcomes. The clinical treatment of dogs with thoracolumbar IVDD is satisfactory, particularly for animals with milder disease grades (I, II, and III). There is possibility of recurrence with conservative therapy and clinical signs may be more severe.

https://doi.org/10.1590/1678-5150-pvb-6067 1425 downloads

12.

OPTIMIZATION OF HIGH-CONCENTRATION TRANS-ANETHOLE PRODUCTION THROUGH HYDRODISTILLATION OF STAR ANISE
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Destro, Bárbara G. I.

; Jorge, Regina M. M.

; Mathias, Alvaro L.

Brazilian Journal of Chemical Engineering

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jun 2019, Volume 36 N. 2 Pages 823 - 830

Abstract: En | Text: En | PDF: En

Abstract Illicium verum Hook essential oil (EO) is composed mainly of trans-anethole (TA), which has therapeutic potential. The extraction is usually by hydrodistillation, taking long hours, leaving the process costly and product with lower quality. Thus, the present study sought to optimize dehydrated fruit fragmentation by Box-Wilson central composite design and EO extraction and EO quality using a 24-1 fractional factorial design. It is concluded that the fruits must be fragmented to sizes smaller than 425 μm using a knife mill. For hydrodistillation, the condition reported as ideal was granulometry < 0.425 mm, 8% mass, 1 hour and water volume of 200 mL. This process provided an EO yield of 10.2% and high-grade TA (96.6%), requiring shorter time of extraction.

https://doi.org/10.1590/0104-6632.20190362s20180203 983 downloads

13.

Performance Analysis of X Band Horn Antennas using Additive Manufacturing Method Coated with Different Techniques
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Chuma, Euclides L.

; Iano, Yuzo

; Roger, Leonardo L. Bravo

; Scroccaro, Mathias

; Frazatto, Felipe

; Manera, Leandro Tiago

Journal of Microwaves, Optoelectronics and Electromagnetic Applications

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jun 2019, Volume 18 N. 2 Pages 263 - 269

Abstract: En | Text: En | PDF: En

Abstract In this study are analyzed horn antennas to operating at X band (8 – 12 GHz) and manufactured by additive manufacturing method (AM). To construct the antennas using AM method was necessary made the structure with acrylonitrile butadiene styrene (ABS) thermoplastic using a 3D printer, and then coated the antennas with conductive surfaces. Were fabricated three antennas with different coated techniques: the first antenna with conductive silver paint, the second antenna using copper tape and the third antenna using copper plated. It is investigated the performances of these antennas in terms of the input reflection coefficient, gain, radiation pattern, weight and compared with the conventional aluminum horn antenna with same dimensions.

https://doi.org/10.1590/2179-10742019v18i21337 1380 downloads

14.

Impact on the physical activity level of adults at high and very high risk of developing type 2 diabetes mellitus: email follow-up intervention
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Montenegro, Carla Giuliano de Sá Pinto

; Pitta, Rafael Mathias

; Marega, Marcio

; Rica, Roberta L.

; Polito, Luis Felipe Tubagi

; Carvalho, José Antonio Maluf de

; Queiroga, Luana

; Trevisani, Mayra

; Ceschini, Fabio

; Bocalini, Danilo Sales

; Figueira Junior, Aylton

Motriz: Revista de Educação Física

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2019, Volume 25 N. 4 elocation e101949

Abstract: En | Text: En | PDF: En

Abstract Aims: The present study aimed to assess the effect of a 24-week intervention on the physical activity level among adults at risk of developing type 2 diabetes mellitus (DM2). Methods: It was included 129 individuals of both sexes who underwent the Preventive Health Check-up at the Albert Einstein Israelita Hospital between January and August 2010 and who were at high and very high risk of developing DM2. The risk of developing DM2 was assessed according to the FINDRISC criteria. All participants were evaluated by the International Physical Activity Questioner (IPAQ). Subjects were followed-up for 24 weeks by a physical education professional through emails during a 6 month period. The IPAQ was sent by email after 3 and 6 months of follow-up. Statistical analysis was performed using the chi-square test with p<0.05 and the Yule Coefficient test. Results: At baseline of the follow-up period, 80.3% of individuals failed to comply with the recommended weekly physical activity. In the end, motivational intervention by e-mail encouraged 74.2% of individuals to comply with the weekly-recommended physical activity and only 1.5% were classified as sedentary. Conclusion: Therefore, the follow-up protocol conducted by periodic and personalized e-mails proved to be effective in promoting physical activity among people at risk of developing metabolic diseases.

https://doi.org/10.1590/s1980-6574201900040001 912 downloads

15.

Characterization and Cytotoxic Evaluation of Silver and Gold Nanoparticles Produced with Aqueous Extract of Lavandula dentata L. in Relation to K-562 Cell Line
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Justus, Barbara

; Arana, Andrés Fernando Montenegro

; Gonçalves, Melissa Marques

; Wohnrath, Karen

; Boscardin, Patrícia Mathias Döll

; Kanunfre, Carla Cristine

; Budel, Jane Manfron

; Farago, Paulo Vitor

; Paula, Josiane de Fátima Padilha de

Brazilian Archives of Biology and Technology

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2019, Volume 62 elocation e19180731

Abstract: En | Text: En | PDF: En

Abstract Metallic nanoparticles have great potential as a chemotherapeutic agent. The aim of this study was to develop and characterize silver and gold nanoparticles using a simple method, as well as evaluating the potential cytotoxic activity in relation to the K-562 cell line. For the synthesis, a solution containing the metallic ions was subjected to magnetic stirring with the aqueous extract of Lavandula dentata L. and a change of colour was observed. With the data obtained from the analyses we concluded that the nanoparticles were successfully obtained by a simple and green method using the aqueous extract of L. dentata. The obtained nanoparticles presented a reduced size, a low level of polydispersion, and a homogenous spherical shape. The nanoparticles presented intense and characteristic diffraction peaks, which could be correlated to the planes of the centred cubic structure of the silver and gold. The two formulations presented predominantly crystalline characteristics. The infrared analysis suggested that the amides and alcohols present in the samples may have been responsible for the reduction and limitation of the size and dispersion of the silver and gold nanoparticles. The cytotoxic assay showed that the nanoparticles demonstrated great potential to reduce the cell viability of the K-562 cell line, especially the gold nanoparticles.

https://doi.org/10.1590/1678-4324-2019180731 622 downloads

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