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1.
Analysis of heart rate as a predictor of changes in heart rate variability in children
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Costa, Paulo César Trindade
; Arruda Neta, Adélia da Costa Pereira de
; Martins, Vinícius José Baccin
; Alves, José Luiz de Brito
.




ABSTRACT Objective: To evaluate the predictive validity and cut-off point of heart rate and blood pressure on heart rate variability (HRV) changes in children with and without obesity. Methods: This study included 125 children, of whom 41 were normal weight and 84 were obese. Anthropometry, blood pressure, heart rate, and HRV were measured using an electronic scale and stadiometer, a sphygmomanometer, and HRV monitor. In addition, the receiver operating characteristic (ROC) curve was obtained by statistical analysis of the data. Results: Heart rate proved to be a good predictor for changes in the square root of the mean of the square of the differences between consecutive NN intervals (RMSSD) parameter in students of both sexes for the normal-weight group (ROC 0.89; 95%CI 0.77–1.00) and obesity (ROC 0.90; 95%CI 0.83–0.97). In addition, the heart rate cut-off point for alterations in the RMSSD parameter for normal-weight boys was 93 bpm (sensitivity 100.00% and specificity 87.50%) and for boys with obesity, the established cut-off point was 91 bpm (sensitivity 94.74% and specificity 63.64%). Heart rate also proved to be a good predictor considering low-frequency/high-frequency ratio (LF/HF) and standard deviation of long-term continuous NN intervals /standard deviation of the instantaneous variability of continuous NN intervals in the Poincaré graph ratio (SD2/SD1). Systolic and diastolic blood pressures were good predictors in more specific stratifications and, therefore, can be used in some cases. Conclusions: The predictive validity of heart rate was shown to be at a good level, with high sensitivity and acceptable specificity for the cut-off points according to the different analyses stratified by gender and nutritional status. In this sense, health professionals will be able to use heart rate to estimate cardiovascular risk in children of different sexes and nutritional status. Objective cutoff cut off (HRV Methods 12 4 8 obese Anthropometry stadiometer sphygmomanometer monitor addition ROC data Results (RMSSD normalweight 0.89 089 0 89 95CI CI 95 0.77–1.00 077100 77 1 00 0.90 090 90 0.83–0.97. 083097 0.83–0.97 . 83 97 0.83–0.97) 9 10000 100 100.00 87.50% 8750 87 50 9474 94 74 94.74 63.64%. 6364 63.64% 63 64 63.64%) lowfrequency/highfrequency lowfrequencyhighfrequency low frequency/high frequency LF/HF LFHF LF HF (LF/HF longterm long term SD2/SD1. SD2SD1 SDSD SD2/SD1 SD2 SD1 SD (SD2/SD1) therefore cases Conclusions level status sense 0.8 08 0.77–1.0 07710 7 0.9 09 08309 0.83–0.9 1000 10 100.0 87.50 875 5 947 94.7 636 63.64 6 lowfrequency highfrequency frequencyhigh SD2SD SD2/SD (SD2/SD1 0. 0.77–1. 0771 0830 0.83–0. 100. 87.5 94. 63.6 (SD2/SD 0.77–1 077 083 0.83–0 87. 63. 0.77– 07 0.83– 0.77 0.83 0.7
RESUMO Objetivo: Avaliar a validade preditiva e o ponto de corte da frequência cardíaca e da pressão arterial nas alterações da variabilidade da frequência cardíaca (VFC) em crianças com e sem obesidade. Métodos: Foram incluídas 125 crianças neste estudo, sendo 41 com peso normal e 84 com obesidade. Antropometria, pressão arterial, frequência cardíaca e VFC foram medidas por meio de balança eletrônica e estadiômetro, esfigmomanômetro e monitor de VFC. Além disso, a curva característica de operação do receptor (ROC) foi obtida pela análise estatística dos dados. Resultados: A frequência cardíaca mostrou-se um bom preditor de alterações no parâmetro de raiz quadrada da média do quadrado das diferenças entre os intervalos NN consecutivos (RMSSD) em escolares de ambos os sexos para o grupo de peso normal (ROC 0,89; IC95% 0,77–1,00) e obesidade (ROC 0,90; IC95% 0,83–0,97). Além disso, o ponto de corte da frequência cardíaca para alterações no parâmetro RMSSD para meninos com peso normal foi de 93 bpm (sensibilidade 100,00% e especificidade 87,50%), e para meninos com obesidade, o ponto de corte estabelecido foi de 91 bpm (sensibilidade 94,74% e especificidade 63,64%). A frequência cardíaca também se mostrou um bom preditor considerando os índices da relação baixa frequência/alta frequência (LF/HF) e razão desvio padrão de intervalos NN contínuos de longo prazo/desvio padrão da variabilidade instantânea de intervalos NN contínuos na relação gráfica de Poincaré (SD2/SD1). As pressões arteriais sistólicas e diastólicas foram bons preditores em estratificações mais específicas e, portanto, podem ser utilizadas em alguns casos. Conclusões: A validade preditiva da frequência cardíaca mostrou-se em bom nível, com alta sensibilidade e especificidade aceitável para os pontos de corte, conforme as diferentes análises estratificadas por sexo e estado nutricional. Desta forma, os profissionais de saúde poderão utilizar a frequência cardíaca para estimar o risco cardiovascular em crianças de diferentes sexos e estado nutricional. Objetivo (VFC Métodos 12 estudo 4 8 Antropometria estadiômetro disso ROC dados Resultados mostrouse (RMSSD 0,89 089 0 89 IC95 IC 0,77–1,00 077100 77 1 00 0,90 090 90 0,83–0,97. 083097 0,83–0,97 . 83 97 0,83–0,97) 9 10000 100 100,00 87,50%, 8750 87,50% , 87 50 87,50%) 9474 94 74 94,74 63,64%. 6364 63,64% 63 64 63,64%) frequênciaalta LF/HF LFHF LF HF (LF/HF prazodesvio prazo SD2/SD1. SD2SD1 SDSD SD2/SD1 SD2 SD1 SD (SD2/SD1) portanto casos Conclusões nível nutricional forma 0,8 08 IC9 0,77–1,0 07710 7 0,9 09 08309 0,83–0,9 1000 10 100,0 875 87,50 5 947 94,7 636 63,64 6 SD2SD SD2/SD (SD2/SD1 0, 0,77–1, 0771 0830 0,83–0, 100, 87,5 94, 63,6 (SD2/SD 0,77–1 077 083 0,83–0 87, 63, 0,77– 07 0,83– 0,77 0,83 0,7
2.
Perfil e Avaliação Social de Candidatos a Transplante de Fígado: Uma Abordagem Retrospectiva Fígado
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Serrano, Luzia Cristina de Almeida
; Pereira, Vinícius Araújo
; Barbeiro, Rafael Mangas
; Duca, William José
; Silva, Rita de Cássia Martins Alves da
; Arroyo Júnior, Paulo César
; Lemes, Jemima Domingos
; Fortunato, Allana C.
; Virches, Adriano
; Miyazaki, Eliane Tiemi
; Sciarra, Adília Maria Pires
; Silva, Renato Ferreira da
.












ABSTRACT Introduction: The complexity of liver transplantation requires a highly qualified team, in which the social worker plays a crucial role in analyzing and intervening in the social situation of candidates. Objectives: To investigate the social profile of liver transplant candidates and relate it to the interventions and reflections made during the social assessment at the Hospital de Base Liver Transplant Unit. Methods: Based on the records of the Social Work, the information of the candidates evaluated between January 2019 and December 2020 was analyzed. This quantitative-qualitative, retrospective, descriptive and documentary study, with participant observation, was conducted from a dialectical perspective. Results: During the data collection period, 174 evaluations were obtained. Social profile: Average age 55.8 years, predominantly male (N=116; 66.7%), with a partner (N=129; 74.1%), living in municipalities in the state of São Paulo (N=124; 71.3%), incomplete primary education (N=68; 39.1%), low level of education (N=65; 37.4%), inactive in the job market (N=151; 86.8%), accessing social security benefits (N=120; 69%), positive acceptance of the transplant (N=158; 90.8%), nuclear family (N=120; 69%), offer of care and family adherence (N=172; 98.9%), partial access to medication (N=122; 70.1%), ease of access to the transplant center (N=157; 90.2%), per person family income of 1\2 to 2 minimum wages (N=107; 61.5%) and satisfactory/conserved housing standard and state of repair (N=157; 90.3%). Conclusion: The social profile of greater social vulnerability required more interventions in most of the 25 variables assessed, providing important elements for identifying and meeting the social needs of each individual. Introduction team Objectives Unit Methods Work 201 202 analyzed quantitativequalitative, quantitativequalitative quantitative qualitative, qualitative quantitative-qualitative retrospective study observation perspective Results period 17 obtained 558 55 8 55. years N=116 N116 N 116 (N=116 66.7%, 667 66.7% , 66 7 66.7%) N=129 N129 129 (N=129 74.1%, 741 74.1% 74 1 74.1%) N=124 N124 124 (N=124 71.3%, 713 71.3% 71 3 71.3%) N=68 N68 68 (N=68 39.1%, 391 39.1% 39 39.1%) N=65 N65 65 (N=65 37.4%, 374 37.4% 37 4 37.4%) N=151 N151 151 (N=151 86.8%, 868 86.8% 86 86.8%) N=120 N120 120 (N=120 69%, 69 69% 69%) N=158 N158 158 (N=158 90.8%, 908 90.8% 90 90.8%) N=172 N172 172 (N=172 98.9%, 989 98.9% 98 9 98.9%) N=122 N122 122 (N=122 70.1%, 701 70.1% 70 70.1%) N=157 N157 157 (N=157 90.2%, 902 90.2% 90.2%) 12 1\ N=107 N107 107 (N=107 61.5% 615 61 5 satisfactoryconserved satisfactory conserved 90.3%. 903 90.3% . 90.3%) Conclusion assessed individual 20 N=11 N11 11 (N=11 66.7 6 N=12 N12 (N=12 74.1 71.3 N=6 N6 (N=6 39.1 37.4 N=15 N15 15 (N=15 86.8 90.8 N=17 N17 (N=17 98.9 70.1 90.2 N=10 N10 10 (N=10 61.5 90.3 N=1 N1 (N=1 66. 74. 71. N= (N= 39. 37. 86. 90. 98. 70. 61. (N
RESUMO Introdução: A complexidade do transplante de fígado requer uma equipe altamente qualificada, no qual o assistente social desempenha papel crucial para analisar e intervir na situação social dos candidatos. Objetivos: Investigar o perfil social dos candidatos ao transplante de fígado e relacioná-lo com as intervenções e reflexões efetuadas durante a avaliação social na Unidade de Transplante de Fígado do Hospital de Base. Métodos: Com base nos registros do Serviço Social, foram analisadas as informações dos candidatos avaliados entre janeiro de 2019 e dezembro de 2020. Este estudo quantitativo-qualitativo, retrospectivo, descritivo e documental, com observação participante, foi conduzido a partir de um panorama dialético. Resultados Durante o período de coleta de dados, obteve-se174 avaliações. Perfil social: média etária de 55,8 anos, predominância masculina (N=116; 66,7%), com companheiro/a (N=129; 74,1%), residentes em municípios do estado de São Paulo (124; 71,3%), ensino fundamental incompleto (N=68, 39,1%), baixo nível de instrução (N=65; 37,4%), inatividade no mercado de trabalho (N=151; 86,8%), acessando benefício da previdência social (N=120; 69%), positiva aceitação do transplante (N=158; 90,8%), família nuclear (N=120; 69%), oferta de cuidados e aderência familiar (N= 172;98,9%), acesso parcial a medicamentos (N=122; 70,1%), facilidade de acesso ao centro transplantador (N=157; 90,2%), renda per capita familiar de 1\2 a 2 salários mínimos (N=107; 61,5%) e padrão habitacional e estado de conservação satisfatório/conservado (N=157; 90,3%). Conclusão O perfil social de maior vulnerabilidade social exigiu mais intervenções na maioria das 25 variáveis avaliadas, fornecendo elementos importantes para a identificação e atendimento das necessidades sociais de cada indivíduo. Introdução qualificada Objetivos relacionálo relacioná lo Base Métodos Social 201 2020 quantitativoqualitativo, quantitativoqualitativo quantitativo qualitativo, qualitativo quantitativo-qualitativo retrospectivo documental participante dialético dados obtevese174 obtevese obteve se174 se obteve-se17 avaliações 558 55 8 55, anos N=116 N116 N 116 (N=116 66,7%, 667 66,7% , 66 7 66,7%) companheiroa companheiro N=129 N129 129 (N=129 74,1%, 741 74,1% 74 1 74,1%) 124 (124 71,3%, 713 71,3% 71 3 71,3%) N=68, N68 68 (N=68 39,1%, 391 39,1% 39 39,1%) N=65 N65 65 (N=65 37,4%, 374 37,4% 37 4 37,4%) N=151 N151 151 (N=151 86,8%, 868 86,8% 86 86,8%) N=120 N120 120 (N=120 69%, 69 69% 69%) N=158 N158 158 (N=158 90,8%, 908 90,8% 90 90,8%) N= (N 17298,9%, 172989 172 98,9% 98 9 172;98,9%) N=122 N122 122 (N=122 70,1%, 701 70,1% 70 70,1%) N=157 N157 157 (N=157 90,2%, 902 90,2% 90,2%) 12 1\ N=107 N107 107 (N=107 61,5% 615 61 5 satisfatórioconservado satisfatório conservado 90,3%. 903 90,3% . 90,3%) avaliadas indivíduo 20 202 obtevese17 se17 obteve-se1 N=11 N11 11 (N=11 66,7 6 N=12 N12 (N=12 74,1 (12 71,3 N=68 N6 (N=6 39,1 N=6 37,4 N=15 N15 15 (N=15 86,8 90,8 17298 17298,9% 17 989 98,9 172;98,9% 70,1 90,2 N=10 N10 10 (N=10 61,5 90,3 obtevese1 se1 obteve-se N=1 N1 (N=1 66, 74, (1 71, 39, 37, 86, 90, 1729 17298,9 98, 172;98,9 70, 61, ( 17298, 172;98, 172;98 172;9 172;
3.
Brazilian consensus recommendations on the diagnosis and treatment of autoimmune encephalitis in the adult and pediatric populations
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Dutra, Lívia Almeida
; Silva, Pedro Victor de Castro
; Ferreira, João Henrique Fregadolli
; Marques, Alexandre Coelho
; Toso, Fabio Fieni
; Vasconcelos, Claudia Cristina Ferreira
; Brum, Doralina Guimarães
; Pereira, Samira Luisa dos Apóstolos
; Adoni, Tarso
; Rocha, Leticia Januzi de Almeida
; Sampaio, Leticia Pereira de Brito
; Sousa, Nise Alessandra de Carvalho
; Paolilo, Renata Barbosa
; Pizzol, Angélica Dal
; Costa, Bruna Klein da
; Disserol, Caio César Diniz
; Pupe, Camila
; Valle, Daniel Almeida do
; Diniz, Denise Sisterolli
; Abrantes, Fabiano Ferreira de
; Schmidt, Felipe da Rocha
; Cendes, Fernando
; Oliveira, Francisco Tomaz Meneses de
; Martins, Gabriela Joca
; Silva, Guilherme Diogo
; Lin, Katia
; Pinto, Lécio Figueira
; Santos, Mara Lúcia Schimtz Ferreira
; Gonçalves, Marcus Vinícius Magno
; Krueger, Mariana Braatz
; Haziot, Michel Elyas Jung
; Barsottini, Orlando Graziani Povoas
; Nascimento, Osvaldo José Moreira do
; Nóbrega, Paulo Ribeiro
; Proveti, Priscilla Mara
; Castilhos, Raphael Machado do
; Daccach, Vanessa
; Glehn, Felipe von
.






































Abstract Background Autoimmune encephalitis (AIE) is a group of inflammatory diseases characterized by the presence of antibodies against neuronal and glial antigens, leading to subacute psychiatric symptoms, memory complaints, and movement disorders. The patients are predominantly young, and delays in treatment are associated with worse prognosis. Objective With the support of the Brazilian Academy of Neurology (Academia Brasileira de Neurologia, ABN) and the Brazilian Society of Child Neurology (Sociedade Brasileira de Neurologia Infantil, SBNI), a consensus on the diagnosis and treatment of AIE in Brazil was developed using the Delphi method. Methods A total of 25 panelists, including adult and child neurologists, participated in the study. Results The panelists agreed that patients fulfilling criteria for possible AIE should be screened for antineuronal antibodies in the serum and cerebrospinal fluid (CSF) using the tissue-based assay (TBA) and cell-based assay (CBA) techniques. Children should also be screened for anti-myelin oligodendrocyte glucoprotein antibodies (anti-MOG). Treatment should be started within the first 4 weeks of symptoms. The first-line option is methylprednisolone plus intravenous immunoglobulin (IVIG) or plasmapheresis, the second-line includes rituximab and/or cyclophosphamide, while third-line treatment options are bortezomib and tocilizumab. Most seizures in AIE are symptomatic, and antiseizure medications may be weaned after the acute stage. In anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis, the panelists have agreed that oral immunosuppressant agents should not be used. Patients should be evaluated at the acute and postacute stages using functional and cognitive scales, such as the Mini-Mental State Examination (MMSE), the Montreal Cognitive Assessment (MoCA), the Modified Rankin Scale (mRS), and the Clinical Assessment Scale in Autoimmune Encephalitis (CASE). Conclusion The present study provides tangible evidence for the effective management of AIE patients within the Brazilian healthcare system. (AIE antigens symptoms complaints disorders young prognosis Academia ABN Sociedade Infantil SBNI, SBNI , SBNI) method 2 neurologists CSF (CSF tissuebased tissue based TBA (TBA cellbased cell CBA (CBA techniques antimyelin anti myelin antiMOG. antiMOG MOG . (anti-MOG) firstline line IVIG (IVIG plasmapheresis secondline second andor cyclophosphamide thirdline third tocilizumab symptomatic stage antiNmethylDaspartate N methyl D aspartate antiNMDAR NMDAR (anti-NMDAR used scales MiniMental Mini Mental MMSE, MMSE (MMSE) MoCA, MoCA (MoCA) mRS, mRS (mRS) CASE. CASE (CASE) system (anti-MOG (MMSE (MoCA (mRS (CASE
Resumo Antecedentes Encefalites autoimunes (EAIs) são um grupo de doenças inflamatórias caracterizadas pela presença de anticorpos contra antígenos neuronais e gliais, que ocasionam sintomas psiquiátricos subagudos, queixas de memória e distúrbios anormais do movimento. A maioria dos pacientes é jovem, e o atraso no tratamento está associado a pior prognóstico. Objetivo Com o apoio da Academia Brasileira de Neurologia (ABN) e da Sociedade Brasileira de Neurologia Infantil (SBNI), desenvolvemos um consenso sobre o diagnóstico e o tratamento da EAIs no Brasil utilizando a metodologia Delphi. Métodos Um total de 25 especialistas, incluindo neurologistas e neurologistas infantis, foram convidados a participar. Resultados Os especialistas concordaram que os pacientes com critérios de possíveis EAIs devem ser submetidos ao rastreio de anticorpos antineuronais no soro e no líquido cefalorraquidiano (LCR) por meio das técnicas de ensaio baseado em tecidos (tissue-based assay, TBA, em inglês) e ensaio baseado em células (cell-based assay, CBA, em inglês). As crianças também devem ser submetidas ao rastreio de de anticorpo contra a glicoproteína da mielina de oligodendrócitos (anti-myelin oligodendrocyte glycoprotein, anti-MOG, em inglês). O tratamento deve ser iniciado dentro das primeiras 4 semanas dos sintomas, sendo as opções de primeira linha metilprednisolona combinada com imunoglobulina intravenosa (IGIV) ou plasmaférese. O tratamento de segunda linha inclui rituximabe e ciclofosfamida. Bortezomib e tocilizumab são opções de tratamento de terceira linha. A maioria das crises epilépticas nas EAIs são sintomáticas, e os fármacos anticrise podem ser desmamadas após a fase aguda. Em relação à encefalite antirreceptor de N-metil-D-aspartato (anti-N-methyl-D-aspartate receptor, anti-NMDAR, em inglês), os especialistas concordaram que agentes imunossupressores orais não devem ser usados. Os pacientes devem ser avaliados na fase aguda e pós-aguda mediante escalas funcionais e cognitivas, como Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), Modified Rankin Scale (mRS), e Clinical Assessment Scale in Autoimmune Encephalitis (CASE). Conclusão Esta pesquisa oferece evidências tangíveis do manejo efetivo de pacientes com EAIs no sistema de saúde Brasileiro. (EAIs gliais subagudos movimento jovem prognóstico ABN (ABN SBNI, SBNI , (SBNI) Delphi 2 infantis participar LCR (LCR tissuebased tissue based assay TBA inglês cellbased cell CBA inglês. . antimyelin anti myelin glycoprotein antiMOG, antiMOG MOG, MOG anti-MOG IGIV (IGIV plasmaférese ciclofosfamida sintomáticas NmetilDaspartato N metil D aspartato antiNmethylDaspartate methyl aspartate receptor antiNMDAR, antiNMDAR NMDAR, NMDAR anti-NMDAR inglês, usados pósaguda pós cognitivas MiniMental Mini Mental MMSE, MMSE (MMSE) MoCA, MoCA (MoCA) mRS, mRS (mRS) CASE. CASE (CASE) Brasileiro (SBNI (MMSE (MoCA (mRS (CASE
4.
Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
Slipinski, Adam
Linzmeier, Adelita M.
Calor, Adolfo R.
Garda, Adrian A.
Kury, Adriano B.
Fernandes, Agatha C.S.
Agudo-Padrón, Aisur I.
Akama, Alberto
Silva Neto, Alberto M. da
Burbano, Alejandro L.
Menezes, Aleksandra
Pereira-Colavite, Alessandre
Anichtchenko, Alexander
Lees, Alexander C.
Bezerra, Alexandra M.R.
Domahovski, Alexandre C.
Pimenta, Alexandre D.
Aleixo, Alexandre L.P.
Marceniuk, Alexandre P.
Paula, Alexandre S. de
Somavilla, Alexandre
Specht, Alexandre
Camargo, Alexssandro
Newton, Alfred F.
Silva, Aline A.S. da
Santos, Aline B. dos
Tassi, Aline D.
Aragão, Allan C.
Santos, Allan P.M.
Migotto, Alvaro E.
Mendes, Amanda C.
Cunha, Amanda
Chagas Júnior, Amazonas
Sousa, Ana A.T. de
Pavan, Ana C.
Almeida, Ana C.S.
Peronti, Ana L.B.G.
Henriques-Oliveira, Ana L.
Prudente, Ana L.
Tourinho, Ana L.
Pes, Ana M.O.
Carmignotto, Ana P.
Wengrat, Ana P.G. da Silva
Dornellas, Ana P.S.
Molin, Anamaria Dal
Puker, Anderson
Morandini, André C.
Ferreira, André da S.
Martins, André L.
Esteves, André M.
Fernandes, André S.
Roza, André S.
Köhler, Andreas
Paladini, Andressa
Andrade, Andrey J. de
Pinto, Ângelo P.
Salles, Anna C. de A.
Gondim, Anne I.
Amaral, Antonia C.Z.
Rondón, Antonio A.A.
Brescovit, Antonio
Lofego, Antônio C.
Marques, Antonio C.
Macedo, Antonio
Andriolo, Artur
Henriques, Augusto L.
Ferreira Júnior, Augusto L.
Lima, Aurino F. de
Barros, Ávyla R. de A.
Brito, Ayrton do R.
Romera, Bárbara L.V.
Vasconcelos, Beatriz M.C. de
Frable, Benjamin W.
Santos, Bernardo F.
Ferraz, Bernardo R.
Rosa, Brunno B.
Sampaio, Brunno H.L.
Bellini, Bruno C.
Clarkson, Bruno
Oliveira, Bruno G. de
Corrêa, Caio C.D.
Martins, Caleb C.
Castro-Guedes, Camila F. de
Souto, Camilla
Bicho, Carla de L.
Cunha, Carlo M.
Barboza, Carlos A. de M.
Lucena, Carlos A.S. de
Barreto, Carlos
Santana, Carlos D.C.M. de
Agne, Carlos E.Q.
Mielke, Carlos G.C.
Caetano, Carlos H.S.
Flechtmann, Carlos H.W.
Lamas, Carlos J.E.
Rocha, Carlos
Mascarenhas, Carolina S.
Margaría, Cecilia B.
Waichert, Cecilia
Digiani, Celina
Haddad, Célio F.B.
Azevedo, Celso O.
Benetti, Cesar J.
Santos, Charles M.D. dos
Bartlett, Charles R.
Bonvicino, Cibele
Ribeiro-Costa, Cibele S.
Santos, Cinthya S.G.
Justino, Cíntia E.L.
Canedo, Clarissa
Bonecker, Claudia C.
Santos, Cláudia P.
Carvalho, Claudio J.B. de
Gonçalves, Clayton C.
Galvão, Cleber
Costa, Cleide
Oliveira, Cléo D.C. de
Schwertner, Cristiano F.
Andrade, Cristiano L.
Pereira, Cristiano M.
Sampaio, Cristiano
Dias, Cristina de O.
Lucena, Daercio A. de A.
Manfio, Daiara
Amorim, Dalton de S.
Queiroz, Dalva L. de
Queiroz, Dalva L. de
Colpani, Daniara
Abbate, Daniel
Aquino, Daniel A.
Burckhardt, Daniel
Cavallari, Daniel C.
Prado, Daniel de C. Schelesky
Praciano, Daniel L.
Basílio, Daniel S.
Bená, Daniela de C.
Toledo, Daniela G.P. de
Takiya, Daniela M.
Fernandes, Daniell R.R.
Ament, Danilo C.
Cordeiro, Danilo P.
Silva, Darliane E.
Pollock, Darren A.
Muniz, David B.
Gibson, David I.
Nogueira, David S.
Marques, Dayse W.A.
Lucatelli, Débora
Garcia, Deivys M.A.
Baêta, Délio
Ferreira, Denise N.M.
Rueda-Ramírez, Diana
Fachin, Diego A.
Souza, Diego de S.
Rodrigues, Diego F.
Pádua, Diego G. de
Barbosa, Diego N.
Dolibaina, Diego R.
Amaral, Diogo C.
Chandler, Donald S.
Maccagnan, Douglas H.B.
Caron, Edilson
Carvalho, Edrielly
Adriano, Edson A.
Abreu Júnior, Edson F. de
Pereira, Edson H.L.
Viegas, Eduarda F.G.
Carneiro, Eduardo
Colley, Eduardo
Eizirik, Eduardo
Santos, Eduardo F. dos
Shimbori, Eduardo M.
Suárez-Morales, Eduardo
Arruda, Eliane P. de
Chiquito, Elisandra A.
Lima, Élison F.B.
Castro, Elizeu B. de
Orlandin, Elton
Nascimento, Elynton A. do
Razzolini, Emanuel
Gama, Emanuel R.R.
Araujo, Enilma M. de
Nishiyama, Eric Y.
Spiessberger, Erich L.
Santos, Érika C.L. dos
Contreras, Eugenia F.
Galati, Eunice A.B.
Oliveira Junior, Evaldo C. de
Gallardo, Fabiana
Hernandes, Fabio A.
Lansac-Tôha, Fábio A.
Pitombo, Fabio B.
Dario, Fabio Di
Santos, Fábio L. dos
Mauro, Fabio
Nascimento, Fabio O. do
Olmos, Fabio
Amaral, Fabio R.
Schunck, Fabio
Godoi, Fábio S. P. de
Machado, Fabrizio M.
Barbo, Fausto E.
Agrain, Federico A.
Ribeiro, Felipe B.
Moreira, Felipe F.F.
Barbosa, Felipe F.
Silva, Fenanda S.
Cavalcanti, Fernanda F.
Straube, Fernando C.
Carbayo, Fernando
Carvalho Filho, Fernando
Zanella, Fernando C.V.
Jacinavicius, Fernando de C.
Farache, Fernando H.A.
Leivas, Fernando
Dias, Fernando M.S.
Mantellato, Fernando
Vaz-de-Mello, Fernando Z.
Gudin, Filipe M.
Albuquerque, Flávio
Molina, Flavio B.
Passos, Flávio D.
Shockley, Floyd W.
Pinheiro, Francielly F.
Mello, Francisco de A.G. de
Nascimento, Francisco E. de L.
Franco, Francisco L.
Oliveira, Francisco L. de
Melo, Francisco T. de V.
Quijano, Freddy R.B.
Salles, Frederico F.
Biffi, Gabriel
Queiroz, Gabriel C.
Bizarro, Gabriel L.
Hrycyna, Gabriela
Leviski, Gabriela
Powell, Gareth S.
Santos, Geane B. dos
Morse, Geoffrey E.
Brown, George
Mattox, George M.T.
Zimbrão, Geraldo
Carvalho, Gervásio S.
Miranda, Gil F.G.
Moraes, Gilberto J. de
Lourido, Gilcélia M.
Neves, Gilmar P.
Moreira, Gilson R.P.
Montingelli, Giovanna G.
Maurício, Giovanni N.
Marconato, Gláucia
Lopez, Guilherme E.L.
Silva, Guilherme L. da
Muricy, Guilherme
Brito, Guilherme R.R.
Garbino, Guilherme S.T.
Flores, Gustavo E.
Graciolli, Gustavo
Libardi, Gustavo S.
Proctor, Heather C.
Gil-Santana, Helcio R.
Varella, Henrique R.
Escalona, Hermes E.
Schmitz, Hermes J.
Rodrigues, Higor D.D.
Galvão Filho, Hilton de C.
Quintino, Hingrid Y.S.
Pinto, Hudson A.
Rainho, Hugo L.
Miyahira, Igor C.
Gonçalves, Igor de S.
Martins, Inês X.
Cardoso, Irene A.
Oliveira, Ismael B. de
Franz, Ismael
Fernandes, Itanna O.
Golfetti, Ivan F.
S. Campos-Filho, Ivanklin
Oliveira, Ivo de S.
Delabie, Jacques H.C.
Oliveira, Jader de
Prando, Jadila S.
Patton, James L.
Bitencourt, Jamille de A.
Silva, Janaina M.
Santos, Jandir C.
Arruda, Janine O.
Valderrama, Jefferson S.
Dalapicolla, Jeronymo
Oliveira, Jéssica P.
Hájek, Jiri
Morselli, João P.
Narita, João P.
Martin, João P.I.
Grazia, Jocélia
McHugh, Joe
Cherem, Jorge J.
Farias Júnior, José A.S.
Fernandes, Jose A.M.
Pacheco, José F.
Birindelli, José L.O.
Rezende, José M.
Avendaño, Jose M.
Duarte, José M. Barbanti
Ribeiro, José R. Inácio
Mermudes, José R.M.
Pujol-Luz, José R.
Santos, Josenilson R. dos
Câmara, Josenir T.
Teixeira, Joyce A.
Prado, Joyce R. do
Botero, Juan P.
Almeida, Julia C.
Kohler, Julia
Gonçalves, Julia P.
Beneti, Julia S.
Donahue, Julian P.
Alvim, Juliana
Almeida, Juliana C.
Segadilha, Juliana L.
Wingert, Juliana M.
Barbosa, Julianna F.
Ferrer, Juliano
Santos, Juliano F. dos
Kuabara, Kamila M.D.
Nascimento, Karine B.
Schoeninger, Karine
Campião, Karla M.
Soares, Karla
Zilch, Kássia
Barão, Kim R.
Teixeira, Larissa
Sousa, Laura D. do N.M. de
Dumas, Leandro L.
Vieira, Leandro M.
Azevedo, Leonardo H.G.
Carvalho, Leonardo S.
Souza, Leonardo S. de
Rocha, Leonardo S.G.
Bernardi, Leopoldo F.O.
Vieira, Letícia M.
Johann, Liana
Salvatierra, Lidianne
Oliveira, Livia de M.
Loureiro, Lourdes M.A. El-moor
Barreto, Luana B.
Barros, Luana M.
Lecci, Lucas
Camargos, Lucas M. de
Lima, Lucas R.C.
Almeida, Lucia M.
Martins, Luciana R.
Marinoni, Luciane
Moura, Luciano de A.
Lima, Luciano
Naka, Luciano N.
Miranda, Lucília S.
Salik, Lucy M.
Bezerra, Luis E.A.
Silveira, Luis F.
Campos, Luiz A.
Castro, Luiz A.S. de
Pinho, Luiz C.
Silveira, Luiz F.L.
Iniesta, Luiz F.M.
Tencatt, Luiz F.C.
Simone, Luiz R.L.
Malabarba, Luiz R.
Cruz, Luiza S. da
Sekerka, Lukas
Barros, Lurdiana D.
Santos, Luziany Q.
Skoracki, Maciej
Correia, Maira A.
Uchoa, Manoel A.
Andrade, Manuella F.G.
Hermes, Marcel G.
Miranda, Marcel S.
Araújo, Marcel S. de
Monné, Marcela L.
Labruna, Marcelo B.
Santis, Marcelo D. de
Duarte, Marcelo
Knoff, Marcelo
Nogueira, Marcelo
Britto, Marcelo R. de
Melo, Marcelo R.S. de
Carvalho, Marcelo R. de
Tavares, Marcelo T.
Kitahara, Marcelo V.
Justo, Marcia C.N.
Botelho, Marcia J.C.
Couri, Márcia S.
Borges-Martins, Márcio
Felix, Márcio
Oliveira, Marcio L. de
Bologna, Marco A.
Gottschalk, Marco S.
Tavares, Marcos D.S.
Lhano, Marcos G.
Bevilaqua, Marcus
Santos, Marcus T.T.
Domingues, Marcus V.
Sallum, Maria A.M.
Digiani, María C.
Santarém, Maria C.A.
Nascimento, Maria C. do
Becerril, María de los A.M.
Santos, Maria E.A. dos
Passos, Maria I. da S. dos
Felippe-Bauer, Maria L.
Cherman, Mariana A.
Terossi, Mariana
Bartz, Marie L.C.
Barbosa, Marina F. de C.
Loeb, Marina V.
Cohn-Haft, Mario
Cupello, Mario
Martins, Marlúcia B.
Christofersen, Martin L.
Bento, Matheus
Rocha, Matheus dos S.
Martins, Maurício L.
Segura, Melissa O.
Cardenas, Melissa Q.
Duarte, Mércia E.
Ivie, Michael A.
Mincarone, Michael M.
Borges, Michela
Monné, Miguel A.
Casagrande, Mirna M.
Fernandez, Monica A.
Piovesan, Mônica
Menezes, Naércio A.
Benaim, Natalia P.
Reategui, Natália S.
Pedro, Natan C.
Pecly, Nathalia H.
Ferreira Júnior, Nelson
Silva Júnior, Nelson J. da
Perioto, Nelson W.
Hamada, Neusa
Degallier, Nicolas
Chao, Ning L.
Ferla, Noeli J.
Mielke, Olaf H.H.
Evangelista, Olivia
Shibatta, Oscar A.
Oliveira, Otto M.P.
Albornoz, Pablo C.L.
Dellapé, Pablo M.
Gonçalves, Pablo R.
Shimabukuro, Paloma H.F.
Grossi, Paschoal
Rodrigues, Patrícia E. da S.
Lima, Patricia O.V.
Velazco, Paul
Santos, Paula B. dos
Araújo, Paula B.
Silva, Paula K.R.
Riccardi, Paula R.
Garcia, Paulo C. de A.
Passos, Paulo G.H.
Corgosinho, Paulo H.C.
Lucinda, Paulo
Costa, Paulo M.S.
Alves, Paulo P.
Roth, Paulo R. de O.
Coelho, Paulo R.S.
Duarte, Paulo R.M.
Carvalho, Pedro F. de
Gnaspini, Pedro
Souza-Dias, Pedro G.B.
Linardi, Pedro M.
Bartholomay, Pedro R.
Demite, Peterson R.
Bulirsch, Petr
Boll, Piter K.
Pereira, Rachel M.M.
Silva, Rafael A.P.F.
Moura, Rafael B. de
Boldrini, Rafael
Silva, Rafaela A. da
Falaschi, Rafaela L.
Cordeiro, Ralf T.S.
Mello, Ramon J.C.L.
Singer, Randal A.
Querino, Ranyse B.
Heleodoro, Raphael A.
Castilho, Raphael de C.
Constantino, Reginaldo
Guedes, Reinaldo C.
Carrenho, Renan
Gomes, Renata S.
Gregorin, Renato
Machado, Renato J.P.
Bérnils, Renato S.
Capellari, Renato S.
Silva, Ricardo B.
Kawada, Ricardo
Dias, Ricardo M.
Siewert, Ricardo
Brugnera, Ricaro
Leschen, Richard A.B.
Constantin, Robert
Robbins, Robert
Pinto, Roberta R.
Reis, Roberto E. dos
Ramos, Robson T. da C.
Cavichioli, Rodney R.
Barros, Rodolfo C. de
Caires, Rodrigo A.
Salvador, Rodrigo B.
Marques, Rodrigo C.
Araújo, Rodrigo C.
Araujo, Rodrigo de O.
Dios, Rodrigo de V.P.
Johnsson, Rodrigo
Feitosa, Rodrigo M.
Hutchings, Roger W.
Lara, Rogéria I.R.
Rossi, Rogério V.
Gerstmeier, Roland
Ochoa, Ronald
Hutchings, Rosa S.G.
Ale-Rocha, Rosaly
Rocha, Rosana M. da
Tidon, Rosana
Brito, Rosangela
Pellens, Roseli
Santos, Sabrina R. dos
Santos, Sandra D. dos
Paiva, Sandra V.
Santos, Sandro
Oliveira, Sarah S. de
Costa, Sávio C.
Gardner, Scott L.
Leal, Sebastián A. Muñoz
Aloquio, Sergio
Bonecker, Sergio L.C.
Bueno, Sergio L. de S.
Almeida, Sérgio M. de
Stampar, Sérgio N.
Andena, Sérgio R.
Posso, Sergio R.
Lima, Sheila P.
Gadelha, Sian de S.
Thiengo, Silvana C.
Cohen, Simone C.
Brandão, Simone N.
Rosa, Simone P.
Ribeiro, Síria L.B.
Letana, Sócrates D.
Santos, Sonia B. dos
Andrade, Sonia C.S.
Dávila, Stephane
Vaz, Stéphanie
Peck, Stewart B.
Christo, Susete W.
Cunha, Suzan B.Z.
Gomes, Suzete R.
Duarte, Tácio
Madeira-Ott, Taís
Marques, Taísa
Roell, Talita
Lima, Tarcilla C. de
Sepulveda, Tatiana A.
Maria, Tatiana F.
Ruschel, Tatiana P.
Rodrigues, Thaiana
Marinho, Thais A.
Almeida, Thaís M. de
Miranda, Thaís P.
Freitas, Thales R.O.
Pereira, Thalles P.L.
Zacca, Thamara
Pacheco, Thaynara L.
Martins, Thiago F.
Alvarenga, Thiago M.
Carvalho, Thiago R. de
Polizei, Thiago T.S.
McElrath, Thomas C.
Henry, Thomas
Pikart, Tiago G.
Porto, Tiago J.
Krolow, Tiago K.
Carvalho, Tiago P.
Lotufo, Tito M. da C.
Caramaschi, Ulisses
Pinheiro, Ulisses dos S.
Pardiñas, Ulyses F.J.
Maia, Valéria C.
Tavares, Valeria
Costa, Valmir A.
Amaral, Vanessa S. do
Silva, Vera C.
Wolff, Vera R. dos S.
Slobodian, Verônica
Silva, Vinícius B. da
Espíndola, Vinicius C.
Costa-Silva, Vinicius da
Bertaco, Vinicius de A.
Padula, Vinícius
Ferreira, Vinicius S.
Silva, Vitor C.P. da
Piacentini, Vítor de Q.
Sandoval-Gómez, Vivian E.
Trevine, Vivian
Sousa, Viviane R.
Sant’Anna, Vivianne B. de
Mathis, Wayne N.
Souza, Wesley de O.
Colombo, Wesley D.
Tomaszewska, Wioletta
Wosiacki, Wolmar B.
Ovando, Ximena M.C.
Leite, Yuri L.R.








ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
5.
Exploring Myrciaria dubia liquid extract as a potential semen extender for breeding roosters
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Silva, Marcia Lorena Monteiro da
; Rufino, João Paulo Ferreira
; Lima, Brenda de Meireles
; Mendonça, Marco Antonio de Freitas
; Chaves, Francisco Alberto de Lima
; Oliveira, Roseane Pinto Martins de
; Costa Neto, Pedro de Queiroz
; Andrade, Paulo Cesar Machado
.








Abstract The current investigation aimed to explore the effects of Myrciaria dubia liquid extract (MDLE) as the primary component of an extender for breeder rooster semen over different periods at room temperature. Fifteen breeder roosters (40 weeks of age, average body weight of 2.05±0.12) with confirmed fertility were used. Employing a factorial design (3x4), the treatments consisted of semen in natura and two semen extenders (an experimental based on MDLE and a commercial) subjected to four periods at room temperature post-collection (5, 10, 15 and 20 minutes) with four replicates (tubes) each. All variables evaluated in this study yielding significant results (p<0.05). Analyzed individually, the experimental extender based on MDLE exhibited a linear reduction (p<0.05) in motility and vigor results, while it caused an increase in pH values and percentages of sperm defects evaluated. When compared with semen in natura and commercial extender, the efficiency of MDLE as a semen extender was inferior to that observed with the commercial extender and similar to the results observed with semen in natura. Nonetheless, the experimental extender based on MDLE yielded satisfactory results for up to 15 minutes of storage time. In conclusion, MDLE can be considered as an alternative for composing a roosters’ semen extender, maintaining sperm characteristics within acceptable limits for up to 15 minutes at room temperature. However, this experimental extender demonstrated lower efficiency than the commercial extender in maintaining the sperm quality at room temperature across all periods tested. (MDLE 40 (4 age 2.05±0.12 205012 2 05 0 12 used 3x4, 3x4 x , (3x4) postcollection post collection 5, 5 (5 10 1 tubes (tubes each p<0.05. p005 p p<0.05 . individually (p<0.05 Nonetheless time conclusion However tested 4 ( 2.05±0.1 20501 3x (3x4 p00 p<0.0 (p<0.0 2.05±0. 2050 (3x p0 p<0. (p<0. 2.05±0 205 p<0 (p<0 2.05± p< (p< 2.05 (p 2.0 2.
6.
Impact of streptozotocin-induced diabetes on experimental masseter pain in rats streptozotocininduced streptozotocin induced
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COSTA, Yuri Martins
; HERCULIANI, Clarissa Carolina Fernandes
; SOARES, Flávia Fonseca Carvalho
; AZEVEDO, Michelle de Campos Soriani
; CONTI, Paulo César Rodrigues
; DIONÍSIO, Thiago José
; OLIVEIRA, Gabriela de Moraes
; FARIA, Flávio Augusto Cardoso de
; SANTOS, Carlos Ferreira
; GARLET, Gustavo Pompermaier
; BONJARDIM, Leonardo Rigoldi
.











Abstract This study aimed to assess the influence of streptozotocin (STZ)-induced diabetes on the nociceptive behavior evoked by the injection of hypertonic saline (HS) into the masseter muscle of rats. Forty male rats were equally divided into four groups: a) isotonic saline control, which received 0.9% isotonic saline (IS), (Ctrl-IS); b) hypertonic saline control, which received 5% HS (Ctrl-HS); c) STZ-induced diabetic, which received IS, (STZ-IS); d) STZ-induced diabetic, which received HS (STZ-HS). Experimental diabetes was induced by a single intraperitoneal injection of STZ at dose of 60 mg/kg dissolved in 0.1 M citrate buffer, and 100 μL of HS or IS were injected into the left masseter to measure the nociceptive behavior. Later on, muscle RNA was extracted to measure the relative expression of the following cytokines: cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF-α), and interleukins (IL)-1β, -2, -6, and -10. One-way analysis of variance (ANOVA) was applied to the data (p < 0.050). We observed a main effect of group on the nociceptive response (ANOVA: F = 11.60, p < 0.001), where the Ctrl-HS group presented the highest response (p < 0.001). However, nociceptive response was similar among the Ctrl-IS, STZ-IS, and STZ-HS group (p > 0.050). In addition, the highest relative gene expression of TNF-α and IL-6 was found in the masseter of control rats following experimental muscle pain (p < 0.050). In conclusion, the loss of somatosensory function can be observed in deep orofacial tissues of STZ-induced diabetic rats. STZinduced (HS groups 09 0 9 0.9 , (IS) CtrlIS Ctrl (Ctrl-IS) b 5 CtrlHS (Ctrl-HS) c STZIS (STZ-IS) d STZHS. STZHS . (STZ-HS) 6 mgkg mg kg 01 1 0. buffer 10 cytokines cyclooxygenase2 cyclooxygenase 2 cyclooxygenase- COX2, COX2 COX (COX-2) TNFα, TNFα TNF α (TNF-α) IL1β, IL1β ILβ IL 1β, 1β β (IL)-1β 2, -2 6, -6 10. -10 Oneway One way ANOVA (ANOVA 0.050. 0050 0.050 050 0.050) 1160 11 11.60 0.001, 0001 0.001 001 0.001) 0.001. However CtrlIS, Ctrl-IS STZIS, STZ-IS addition IL6 IL- conclusion (IS (Ctrl-IS (Ctrl-HS (STZ-IS (STZ-HS (COX-2 (TNF-α - -1 005 0.05 05 116 11.6 000 0.00 00 (COX- 0.0 11. (COX
7.
VATICAN (Ventilator-Associated Tracheobronchitis Initiative to Conduct Antibiotic Evaluation): protocol for a multicenter randomized open-label trial of watchful waiting versus antimicrobial therapy for ventilator-associated tracheobronchitis VentilatorAssociated Ventilator Associated Evaluation Evaluation) openlabel open label ventilatorassociated ventilator associated
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Tomazini, Bruno Martins
; Besen, Bruno Adler Maccagnan Pinheiro
; Dietrich, Camila
; Gandara, Ana Paula Rossi
; Silva, Debora Patrícia
; Pinheiro, Carla Cristina Gomes
; Luz, Mariane Nascimento
; Mattos, Renata Rodrigues de
; Reis, Luiz Fernando Lima
; Roepke, Roberta Muriel Longo
; Duarte, Carlos Sérgio Luna Gomes
; Nassar Júnior, Antônio Paulo
; Veiga, Viviane Cordeiro
; Arns, Beatriz
Nascimento, Giovanna Marssola
Pereira, Adriano José
Cavalcanti, Alexandre Biasi
Machado, Flávia Ribeiro
Azevedo, Luciano Cesar Pontes













ABSTRACT Background Ventilator-associated tracheobronchitis is a common condition among invasively ventilated patients in intensive care units, for which the best treatment strategy is currently unknown. We designed the VATICAN (Ventilator-Associated Tracheobronchitis Initiative to Conduct Antibiotic Evaluation) trial to assess whether a watchful waiting antibiotic treatment strategy is noninferior to routine antibiotic treatment for ventilator-associated tracheobronchitis regarding days free of mechanical ventilation. Methods VATICAN is a randomized, controlled, open-label, multicenter noninferiority trial. Patients with suspected ventilator-associated tracheobronchitis without evidence of ventilator-associated pneumonia or hemodynamic instability due to probable infection will be assigned to either a watchful waiting strategy, without antimicrobial administration for ventilator-associated tracheobronchitis and prescription of antimicrobials only in cases of ventilator-associated pneumonia, sepsis or septic shock, or another infectious diagnosis, or to a routine antimicrobial treatment strategy for seven days. The primary outcome will be mechanical ventilation-free days at 28 days, and a key secondary outcome will be ventilator-associated pneumonia-free survival. Through an intention-to-treat framework with a per-protocol sensitivity analysis, the primary outcome analysis will address noninferiority with a 20% margin, which translates to a 1.5 difference in ventilator-free days. Other analyses will follow a superiority analysis framework. Conclusion The VATICAN trial will follow all national and international ethical standards. We aim to publish the trial in a high-visibility general journal and present it at critical care and infectious disease conferences for dissemination. These results will likely be immediately applicable to the bedside upon trial completion and will provide information with a low risk of bias for guideline development. Ventilatorassociated Ventilator associated units unknown VentilatorAssociated Associated Evaluation ventilatorassociated ventilator ventilation randomized controlled openlabel, openlabel open label, label open-label shock diagnosis ventilationfree 2 pneumoniafree survival intentiontotreat intention treat perprotocol per protocol 20 margin 15 1 5 1. ventilatorfree standards highvisibility high visibility dissemination development
RESUMO Contexto A traqueobronquite associada ao ventilador é uma condição comum entre pacientes ventilados invasivamente em unidades de terapia intensiva, para a qual se desconhece atualmente a melhor estratégia de tratamento. Desenhamos o estudo VATICAN (Ventilator-Associated Tracheobronchitis Initiative to Conduct Antibiotic Evaluation) para avaliar se uma estratégia de tratamento antibiótico de espera vigilante não é inferior ao tratamento antibiótico de rotina para traqueobronquite associada ao ventilador em relação aos dias sem ventilador mecânico. Métodos O VATICAN é um estudo randomizado, controlado, aberto e multicêntrico de não inferioridade. Os pacientes com suspeita de traqueobronquite associada ao ventilador sem evidência de pneumonia associada ao ventilador ou instabilidade hemodinâmica devido a uma provável infecção serão designados para uma estratégia de espera vigilante, sem administração profilática de antimicrobianos contra traqueobronquite associada ao ventilador e prescrição de antimicrobianos somente em casos de pneumonia associada ao ventilador, sepse ou choque séptico, ou outro diagnóstico infeccioso, ou para uma estratégia de tratamento antimicrobiano de rotina por 7 dias. O desfecho primário será o número de dias sem ventilador mecânico em 28 dias, e um desfecho secundário importante será a sobrevida sem pneumonia associada ao ventilador. Por meio de uma estrutura de intenção de tratar com análise de sensibilidade por protocolo, a análise do desfecho primário abordará a não inferioridade com margem de 20%, o que se traduz em uma diferença de 1,5 dia sem ventilador. Outras análises seguirão uma estrutura de análise de superioridade. Conclusão O VATICAN seguirá todos os padrões éticos nacionais e internacionais. O objetivo é publicar o estudo em um periódico geral de alta visibilidade e apresentá-lo em conferências de cuidados intensivos e doenças infecciosas para divulgação. Estes resultados provavelmente serão imediatamente aplicáveis à beira do leito após a conclusão do estudo e fornecerão informações com baixo risco de viés para o desenvolvimento de diretrizes. intensiva VentilatorAssociated Ventilator Associated Evaluation randomizado controlado séptico infeccioso 2 protocolo 20 20% 15 1 5 1, superioridade internacionais apresentálo apresentá lo divulgação diretrizes
8.
Littoral cell angioma of the spleen: case report and literature review spleen
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Machado Júnior, Paulo André Bispo
; Pereira, Caroline de Oliveira
; Bortolo, Julia Letícia de
; Martins, Ana Luísa Caetano Lopes
; Campos, Helder Groenwold
; Aguiar, Alan Junior de
; Postai, Rayana Pecharki Teixeira Alves
; Wiederkehr, Julio Cesar
; Wiederkehr, Henrique de Aguiar
.









ABSTRACT Littoral cell angioma is an extremely rare splenic vascular tumor originating from the cells lining the splenic red pulp sinuses. Approximately 150 cases of littoral cell angioma have been reported since 1991. Its clinical manifestation is usually asymptomatic and is mostly diagnosed as an incidental finding through abdominal imaging. Herein, we present a case of littoral cell angioma in a 41-year-old woman with no previous comorbidities, which initially presented as a nonspecific splenic lesion diagnosed on imaging in the emergency room. The patient was treated through laparoscopic intervention. sinuses 15 1991 Herein 41yearold yearold 41 year old comorbidities room intervention 1 199 4 19
9.
Evaluation of cardiac autonomic function and low-grade inflammation in children with obesity living in the Northeast Brazilian region lowgrade low grade
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Costa, Paulo César Trindade
; Souza, Joelma Rodrigues de
; Lima, Poliana Correia
; Duarte, Davyson Barbosa
; Neves, Thallyta Alanna Ferreira Viana das
; Pereira, Joicy Karla Grangeiro
; Silva-Luis, Cristiane Cosmo
; Moraes, Rúbia Cartaxo Squizato de
; Braga, Valdir de Andrade
; Souza, Evandro Leite de
; Martins, Vinícius José Baccin
; Alves, José Luiz de Brito
.












Abstract Objective Evaluate autonomic function and low-grade inflammation and characterize the correlation between these variables in schoolchildren with obesity living in the Brazilian northeast region. Methods 84 children with obesity and 41 with normal weight were included in this cross-sectional study. Anthropometry, body composition, blood pressure (BP), inflammatory biomarkers, and heart rate variability (HRV) indexes were analyzed in children aged 7 to 11 years. Results children with obesity had increased systolic (p= 0.0017) and diastolic (p= 0.0131) BP and heart rate (p= 0.0022). The children with obesity displayed significantly lower SDNN, RMSSD, NN50, HF (ms), HF (nu), SD1, SD2, and higher LF (ms), LF (nu), LF/HF, SD1/SD2, DFA-α1, and DFA-α2, compared to normal weight. A lower and higher capacity for producing IL-10 (p= 0.039) and IL-2 (p= 0.009), respectively, were found in children with obesity compared to children with normal weight. Although IL-2, IL-4 and IL17A did not correlate with HRV parameters, IL-6 was positively correlated with SDNN, LF (ms) and SD2, TNF-α was positively correlated with LF/HF and SD1/SD2 ratio, and IFN-γ was positively correlated with SDNN, RMMSSD, NN50, LF (ms), HF (ms), SD1, and SD2. Conclusions The findings suggest that children with obesity have impaired autonomic function and systemic low-grade inflammation compared to children within the normal weight range, the inflammatory biomarkers were correlated with HRV parameters in schoolchildren living in the northeastern region of Brazil. lowgrade low grade 8 4 crosssectional cross sectional study Anthropometry composition BP, , (BP) (HRV 1 years p= p (p 0.0017 00017 0 0017 0.0131 00131 0131 0.0022. 00022 0.0022 . 0022 0.0022) SDNN RMSSD NN50 NN ms, ms nu, nu (nu) SD1 SD SD2 LFHF SD1SD2 SDSD DFAα1, DFAα1 DFAα DFA α1, α1 α DFA-α1 DFAα2, DFAα2 α2, α2 DFA-α2 IL10 IL 10 IL-1 0.039 0039 039 IL2 2 IL- 0.009, 0009 0.009 009 0.009) respectively IL2, 2, IL4 ILA IL6 6 (ms TNFα TNF SD1/SD ratio IFNγ IFN γ RMMSSD range Brazil (BP 0.001 0001 001 0.013 0013 013 0002 0.002 002 NN5 (nu SD1SD DFA-α IL1 0.03 003 03 000 0.00 00 0.01 01 0.0 0.
10.
Duloxetine in addition to self-management for painful temporomandibular disorders: a post hoc responder analysis of a randomized, placebo-controlled clinical trial selfmanagement self management disorders randomized placebocontrolled placebo controlled
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FERREIRA, Dyna Mara Araújo Oliveira
; SOARES, Flávia Fonseca Carvalho
; RAIMUNDINI, Amanda Ayla
; BONJARDIM, Leonardo Rigoldi
; COSTA, Yuri Martins
; CONTI, Paulo César Rodrigues





Abstract Aim To identify the phenotypic characteristics of individuals with temporomandibular disorders (TMD) who may benefit from adding duloxetine to self-management (SM) strategies. Methodology This was a post hoc exploratory analysis of a randomized, placebo-controlled clinical trial with SM-duloxetine (duloxetine 60 mg/day plus SM strategies for 12 weeks) in adult participants with painful TMD. The primary outcome was the proportion of responders to treatment (individuals with ≥ 30% reduction in pain intensity) in SM-duloxetine and SM-placebo group at week 12. For responder analysis, five phenotyping domains recommended by Initiative on Methods, Measurement, and Pain Assessment in Clinical Trials were assessed: pain, psychological, sleep, quantitative sensory testing, and conditioned pain modulation. Relative risk (RR), 95% confidence intervals (CI), and absolute risk reduction were calculated. Results Among participants treated with SM-duloxetine, severe pain intensity (RR 1.33, 95% CI: 0.56, 3.17), pain disability (RR 1.30, 95% CI: 0.63, 2.67), ≥ 1 painful comorbidity (RR 1.48, 95% CI: 0.57, 3.79), and anxiety symptoms (RR 1.80, 95% CI: 0.75, 4.34) were associated with greater likelihood of response to treatment. Among individuals treated with SM-placebo, only temporal summation of pain was associated with greater likelihood of response to treatment. Conclusion Personalized medicine may be implemented in painful TMD management, and phenotype characteristics related to pain and psychological domains may predict which individuals with painful TMD are more likely to respond to the addition of serotonin and norepinephrine reuptake inhibitors to SM strategies to clinically and significantly reduce pain intensity. (TMD selfmanagement self management (SM randomized placebocontrolled placebo controlled SMduloxetine 6 mgday mg day weeks 30 SMplacebo Methods Measurement assessed sleep testing modulation RR, RR , (RR) 95 CI, CI (CI) calculated SMduloxetine, duloxetine, 133 33 1.33 056 0 56 0.56 3.17, 317 3.17 3 17 3.17) 130 1.30 063 63 0.63 2.67, 267 2.67 2 67 2.67) 148 48 1.48 057 57 0.57 3.79, 379 3.79 79 3.79) 180 80 1.80 075 75 0.75 4.34 434 4 34 SMplacebo, placebo, 9 (CI 13 1.3 05 5 0.5 31 3.1 06 0.6 26 2.6 14 1.4 37 3.7 7 18 8 1.8 07 0.7 4.3 43 1. 0. 3. 2. 4.
11.
Beckwith-Wiedemann syndrome mimicking the classical form of congenital adrenal hyperplasia in newborn screening BeckwithWiedemann Beckwith Wiedemann
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Martins, Jéssica Mallmann Erbes Schaefer
; Braga, Barbara Leitao
; Sampaio, Klevia Nunes Feitosa
; de Souza Garcia, Tamires
; Van de Sande Lee, Juliana
; Cechinel, Edson
; Simoni, Genoir
; Nascimento, Marilza Leal
; da Silva, Paulo Cesar Alves
; Fragoso, Maria C. V.
; Bachega, Tania A. A. S.
; Nishi, Mirian Y.
; Mendonca, Berenice B.
.













SUMMARY Beckwith-Wiedemann syndrome (BWS) is a common genetic congenital disease characterized by somatic overgrowth and its broad clinical spectrum includes pre- and post-natal macrosomia, macroglossia, visceromegaly, increased risk of neonatal hypoglycemia, and development of embryonic tumors. BWS occurs due to genetic/epigenetic changes involving growth-regulating genes, located on region 11p15, with an important genotype-phenotype correlation. Congenital adrenal hyperplasia (CAH) comprises a spectrum of autosomal recessive diseases presenting a variety of clinical manifestations due to a deficiency in one of the enzymes involved in cortisol secretion. Early diagnosis based on newborn screening prevents the adrenal crisis and early infant death. However, high 17-hydroxyprogesterone (17-OHP) levels can occur in newborns or premature infants without CAH, in situations of stress due to maternal or neonatal factors. Here, we report new cases of false-positive diagnosis of 21-hydroxylase deficiency during newborn screening – two girls and one boy with BWS. Methylation-specific multiplex ligation-dependent probe amplification revealed a gain of methylation in the H19 differentially methylated region. Notably, all three cases showed a complete normalization of biochemical changes, highlighting the transient nature of these hormonal findings that imitate the classical form of CAH. This report sheds light on a new cause of false-positive 21-hydroxylase deficiency diagnosis during newborn screening: Beckwith-Wiedemann syndrome. BeckwithWiedemann Beckwith Wiedemann (BWS pre postnatal post natal macrosomia macroglossia visceromegaly hypoglycemia tumors geneticepigenetic epigenetic growthregulating growth regulating genes 11p15 p genotypephenotype genotype phenotype correlation CAH (CAH secretion death However 17hydroxyprogesterone hydroxyprogesterone 17 17OHP OHP (17-OHP factors Here falsepositive false positive 21hydroxylase hydroxylase 21 Methylationspecific Methylation specific ligationdependent ligation dependent H H1 Notably 11p1 1 2 11p
12.
Oxidative stress and neurotoxicity in Scolelepis goodbodyi (Polychaeta, Spionidae) after an experimental oil spill in a dissipative sandy beach Polychaeta, Polychaeta (Polychaeta Spionidae
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Ozorio, Carla Penna
; Guiloski, Izonete C.
; Silva de Assis, Helena C.
; Martins, César C.
; Sandrini-Neto, Leonardo
; Lana, Paulo C.
.






Abstract Biomarkers of environmental contamination have been frequently used in the assessment of marine ecosystem quality because they provide quantitative measures of biological changes in organisms exposed to pollutants such as hydrocarbons from oil spills. Polychaetes have been tested as sentinel organisms of marine environmental health because they are abundant taxa of benthic assemblages and their sedentary lifestyle ensures chronic exposure to toxins in impacted areas. In this study, we evaluated whether the polychaete Scolelepis goodbodyi can be used as a reliable sentinel species for exposure to polycyclic aromatic hydrocarbons (PAHs) from an experimental diesel spill in a dissipative sand beach in the Southern Atlantic. The design used in this study comprised replicated control and diesel impact sites sampled four times (one, two, four and seven days) before and after the impact. Total PAH levels reached 114.0 ng g -1 in the impact site one day after the diesel spill; however, the pattern of biomarker responses in S. goodbodyi was primarily influenced by spatial variation rather than being attributed to the simulated diesel spill. The apparent absence of a contaminating effect may be linked to the low levels of the toxins retained in the sediment after the spill was simulated to elicit a response or to the presence of an efficient repair system within the organism. Furthermore, the sediments tested in this study, composed of sandy fractions, had a low capacity to concentrate PAHs after the simulated diesel spill, which may have contributed to the low significant changes in their biomarker activities. Based on our results, further studies testing other Polychaeta species and simulating oil spills in sedimentary environments composed of fine sediments such as salt marshes and mangroves may help produce evidence on more effective biomarker responses in these organisms. areas (PAHs Atlantic one, (one two days 1140 114 0 114. 1 - however S organism Furthermore fractions activities results 11
13.
Cytotoxic effects of crotoxin from Crotalus durissus terrificus snake in canine mammary tumor cell lines
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Pedro, Giovana
; Brasileiro, Felipe César da Silva
; Macedo, Jamile Mariano
Soares, Andreimar Martins
Mafra, Gabriel Caporale
Alves, Carlos Eduardo Fonseca
Laufer-Amorim, Renée


Journal of Venomous Animals and Toxins including Tropical Diseases
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Abstract Background: Mammary gland tumors are the most prevalent neoplasm in intact female dogs, and they are good natural models to study comparative oncology. Most canine mammary malignancies, as in women, are commonly refractory to conventional therapies and demand continuous new therapeutic approaches. Crotalus durissus terrificus, also called rattlesnake, has more than 60 different proteins in its venom with multiple pharmaceutical uses, such as antitumor, antiviral, and antimicrobial action. Crotoxin, a potent β-neurotoxin formed by the junction of two subunits, a basic subunit (CB-PLA2) and an acidic subunit (crotapotin), has already been reported to have anticancer properties in different types of cancers. Methods: In this work, we describe the cytotoxic potential of crotoxin and its subunits compared to doxorubicin (drug of choice) in two canine mammary carcinoma cell lines. Results: Crotoxin, CB-PLA2, crotalic venom, and doxorubicin decreased cell viability and the ability to migrate in a dose-dependent manner, and crotapotin did not present an antitumoral effect. For all compounds, the predominant cell death mechanism was apoptosis. In addition, crotoxin did not show toxicity in normal canine mammary gland cells. Conclusion: Therefore, this work showed that crotoxin and CB-PLA2 had cytotoxic activity, migration inhibition, and pro-apoptotic potential in canine mammary gland carcinoma cell lines, making their possible use in cancer research. Background dogs oncology malignancies women approaches terrificus rattlesnake 6 uses antitumor antiviral action Crotoxin βneurotoxin β neurotoxin CBPLA2 CBPLA CB PLA2 PLA (CB-PLA2 crotapotin, , (crotapotin) cancers Methods drug choice lines Results CBPLA2, PLA2, dosedependent dose dependent manner effect compounds apoptosis addition cells Conclusion Therefore CB-PLA activity inhibition proapoptotic pro apoptotic research (CB-PLA (crotapotin
14.
Seroconversion and seroreversion rates of anti-Strongyloides IgG in rural areas of the Amazon: a population-based panel study antiStrongyloides anti Strongyloides Amazon populationbased population based
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Paula, Fabiana Martins de
; Gomes, Bruna Barroso
; Meisel, Dirce Mary Correia Lima
; Roldan, William Henry
; Nunes, Mônica da Silva
; Ferreira, Marcelo Urbano
; Gryschek, Ronaldo Cesar Borges
.







Revista do Instituto de Medicina Tropical de São Paulo
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ABSTRACT Using a panel study design, we aimed to estimate the seroconversion and seroreversion rates of anti-Strongyloides IgG antibodies from surveys carried out 11 months apart in a rural community in the Amazon Basin in Brazil. We used enzyme immunoassays to measure anti-Strongyloides IgG antibodies in 325 baseline plasma samples and 224 others that were collected 11 months later from residents in the agricultural settlement of Granada, Acre State. We observed anti-Strongyloides IgG antibodies in 21.8% of the baseline samples (which showed that 3.4% of participants had larvae in their stool) and in 23.7% of the follow-up samples. The seroconversion rate estimated at 9.7 episodes/100 person-years at risk agrees with ongoing transmission. Specific antibodies were relatively short-lived and nine (25.0%) of 36 seropositive participants at baseline were seronegative when retested 11 months later. Fecal surveys can severely underestimate the prevalence of S. stercoralis infection in rural Amazonians. Serology provides a field-deployable diagnostic tool to find high-prevalence populations, identify associated risk factors, and monitor intervention programs. design antiStrongyloides anti Strongyloides 1 Brazil 32 22 Granada State 218 21 8 21.8 which 34 3 4 3.4 stool 237 23 7 23.7 followup follow up 97 9 9. episodes100 episodes 100 episodes/10 personyears person years transmission shortlived short lived 25.0% 250 25 0 (25.0% S Amazonians fielddeployable field deployable highprevalence high populations factors programs 2 21. 3. 23. episodes10 10 episodes/1 25.0 (25.0 episodes1 episodes/ 25. (25. (25 (2 (
15.
Insecticidal and antifeedant bioactivities of Melaleuca alternifolia essential oil on Ascia monuste orseis
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Martins Da Silva, Paulo Henrique
; Prado, Evandro Pereira
; Ferreira-Filho, Pedro José
; Francisco, João Paulo
; Quiqui, Erci Marcos Del
; Silva, Camila
; Guerreiro, Julio César
.







Resumen Este estudio evaluó los efectos bioactivos del aceite esencial de Melaleuca alternifolia sobre el comportamiento y la mortalidad de las orugas Ascia monuste orseis. El experimento se llevó a cabo en el laboratorio bajo condiciones controladas utilizando un diseño completamente al azar con seis tratamientos, representados por orugas de A. monuste orseis alimentadas con hojas de berza tratadas con 0 (control), 5, 10, 20, 30 o 40 mg mL-1 aceite esencial, los tratamientos se aplicaron a discos de hojas de col por inmersión durante 1 min. Las hojas se ofrecieron a los insectos ad libitum. Cada tratamiento consistió en cinco repeticiones de cuatro orugas de tercer estadio. Se evaluó la ingesta diaria media de hojas de berza, la producción de heces, la mortalidad y los cambios de comportamiento durante 15 días después del comienzo del experimento. Los datos sobre el consumo de hojas y la producción de heces se sometieron a análisis de varianza mediante la prueba F; cuando se encontraron diferencias significativas, los datos se sometieron a análisis de regresión. Se calculó la tasa de mortalidad corregida y se sometió a análisis de varianza mediante la prueba F seguida de la prueba de Tukey al nivel de significancia del 5 % para la comparación de medias. También se evaluó la concentración letal mediana (CL50). Se observaron dos comportamientos de alimentación muy claros, uno en orugas expuestas a concentraciones de aceite esencial de 5, 10 y 20 mg mL-1, que mostraron poca diferencia en el consumo de alimento del control, y el otro en orugas expuestas a las concentraciones más altas (30 y 40 mg mL-1), que mostró una disminución de 76 % a 93 % en el consumo de alimento en comparación con el control. Una menor ingesta de hojas resultó en una disminución en la producción de heces y los efectos negativos aumentaron linealmente con la concentración de aceite esencial. La curva de mortalidad mostró una respuesta lineal y positiva a la concentración de aceite esencial, alcanzando el 100 % en los insectos expuestos a las concentraciones más altas. La CL50 fue de 13,93 mg mL-1.
Abstract The bioactive effects of Melaleuca alternifolia essential oil on the behavior and mortality of Ascia monuste orseis caterpillars were studied. The experiment was conducted under controlled laboratory conditions using a completely randomized design with six treatments, represented by A. monuste orseis caterpillars fed on cabbage leaves treated with 0 (control), 5, 10, 20, 30, or 40 mg mL-1 essential oil. Treatments were applied to discs of collard leaves by immersion for 1 min. The leaves were offered to the insects ad libitum. Each treatment consisted of five replicates of four third-instar caterpillars. Mean daily intake of collard leaves, faeces production, mortality, and behavioral changes were assessed for 15 days after the beginning of the experiment. Data on leaf intake and faeces production were subjected to analysis of variance by the F-test; when significant differences were found, data were subjected to regression analysis. Corrected mortality rate was calculated and subjected to analysis of variance by the F-test followed by Tukey’s test at the 5 % significance level for comparison of means. The median lethal concentration (LC50) was also evaluated. Two very clear feeding behaviors were observed, one in caterpillars exposed to essential oil concentrations of 5, 10, and 20 mg mL-1, which showed little difference in leaf intake from the control, and the other in caterpillars exposed to the highest concentrations (30 and 40 mg mL-1), which showed a decrease of 76 % to 93 % in feed intake compared with the control. Lower leaf intake resulted in a decrease in faeces production, and the negative effects increased linearly with essential oil concentration. The mortality curve showed a linear and positive response to essential oil concentration, reaching 100% in insects exposed to the highest concentrations. The LC50 was 13.93 mg mL-1.
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