Resumo Objetivos: investigar o efeito da imunoterapia orofaríngea de colostro (IOC) na incidência de morte em recém-nascido pré-termo. Métodos: trata-se de uma revisão sistemática com metanálise, com protocolo do estudo registrado no PROSPERO. As bases de dados utilizadas foram: Lilacs, Pubmed, Web of Science, Portal CAPES, Scopus, REBEC e Clinical Trial; e, incluídos estudos publicados de 2007 a 2024, sem limitação de idiomas. Foram selecionados ensaios clínicos randomizados que utilizaram IOC em prematuros e apresentaram como desfecho morte. As etapas da revisão consistiram em: produção do protocolo, registro, elegibilidade dos estudos, extração dos dados, avaliação da qualidade dos estudos, síntese dos dados e metanálise. Os softwares utilizados foram: Rayyan e Stata 10.0. Resultados: foram incluídos 15 estudos para análise qualitativa e quantitativa. No que concerne a avaliação do risco global, os estudos foram considerados como alto risco de viés. Na análise quantitativa, a amostra foi composta por 1.497 prematuros; e, a medida meta-analítica mostrou uma diferença da média de RR= 0,750 (IC95%= 0,582-0,967, sem evidências de heterogeneidade entre os estudos (I2=0,0%; p=0,649). Conclusão: a medida meta-analítica sugere um possível efeito positivo da IOC na redução da mortalidade neonatal de prematuros.

Abstract Objectives: to investigate the effect of oropharyngeal colostrum immunotherapy (OCI) on death incidence in preterm newborns. Methods: this is a systematic review with meta-analysis, a study protocol registered in PROSPERO. The databases used were: Lilacs, Pubmed, Web of Science, Portal Capes, Scopus, REBEC and Clinical Trial; and, included studies published from 2007 to 2024, without language limitations. Randomized clinical trials that used OCI in premature infants and had death as an outcome, were selected. The review stages consisted of: protocol production, registration, study eligibility, data extraction, study quality assessment, data synthesis and meta-analysis. The software used was: Rayyan and Stata 10.0. Results: 15 studies were included for qualitative and quantitative analysis. Regarding the assessment of global risk, the studies were considered to have a high risk of bias. In the quantitative analysis, the sample consisted of 1,497 premature infants; and, the meta-analytic measure showed a difference from the mean of RR=0.750 (95%CI=0.582-0.967), without evidence of heterogeneity between studies I2=0.0%; p=0.649 and positive trend in preventing the incidence of death in the intervention group compared to the control group. Conclusion: the meta-analytic measurement suggests a possible positive effect of OCI in reducing neonatal mortality in premature infants.

Abstract Objectives: to evaluate the effect of oropharyngeal colostrum immunotherapy in reducing cases of late-onset neonatal sepsis in preterm infants with very low birth weight. Methods: this is an intervention study, with a comparative analysis between the incidence of late-onset neonatal sepsis in the treatment group (in use of oropharyngeal colostrum immunotherapy) and the historical control group (newborns monitored in the same intensive care unit prior to the implementation of the oropharyngeal colostrum immunotherapy protocol). 81 premature babies born between 2016 and 2022 participated in the study separated according to whether or not they received oropharyngeal colostrum immunotherapy. The intervention consisted of eight daily applications of 0.2 mL of the mother's own raw colostrum to the newborns oral mucosa during the first seven days of life. Relative Risk and Absolute Risk Reduction and Number needed to Treat were calculated. Results: a protective effect of oropharyngeal colostrum immunotherapy against late neonatal sepsis was noted. Relative Risk: 0.43; CI95% = 0.21-0.91; Absolute Risk Reduction: 0.26; CI95%= 6.51 - 45.92 and Number Needed to Treat: 4 (2.17-15.34). Conclusion: administration of oropharyngeal colostrum proved to be a promising measure in protecting preterm newborns with VLBW against late-onset sepsis.

Resumo Objetivos: avaliar o efeito da Imunoterapia Orofaríngea de Colostro na redução dos casos de sepse neonatal tardia, em recém-nascidos prematuros com muito baixo peso. Métodos: trata-se de um estudo de intervenção, com análise comparativa entre a incidência de sepse neonatal tardia do grupo tratamento (em uso da Imunoterapia Orofaríngea de Colostro) e grupo controle histórico (recém-nascidos acompanhados na mesma unidade de terapia intensiva neonatal, anteriormente à implementação do protocolo de Imunoterapia Orofaríngea de Colostro). Participaram do estudo 81 prematuros nascidos entre 2016 e 2022, separados de acordo com o recebimento ou não da Imunoterapia Orofaríngea de Colostro. A intervenção consistiu em oito aplicações diárias de 0,2 mL de colostro cru da própria mãe na mucosa oral dos participantes, durante os sete primeiros dias de vida. Foi feito cálculo de Risco Relativo e Redução Absoluto de Risco e do Número Necessário para Tratar. Resultados: notou-se efeito protetor da IOC contra a sepse neonatal tardia. Risco relativo: 0,43; IC95%=0,21-0,91; Redução absoluta de risco: 0,26; IC95%=6,51-45,92 e Número necessário para tratar: 4 (2,17-15,34). Conclusão: a administração orofaríngea de colostro se mostrou uma medida promissora na proteção de RN PT com MBP contra sepse tardia.

Abstract Objectives: to evaluate the effect of oropharyngeal colostrum immunotherapy on the length of hospital stay in preterm newborns with very low birth weight. Methods: interventional ambispective study, which consisted of eight daily administrations of 0.2 ml (four drops) of colostrum, totaling up to 56 syringes (for up to seven days). The control was historic. The main independent variable: length of hospital stay (days). Survival analysis was performed using the Kaplan-Meier Method and the survival effect was estimated - Log Rank Test (Mantel-Cox) and Breslow Test (Generalized Wilcoxon). A significance level of 5% was adopted. Results: of the 109 mother/child pairs, 56 were part of the treatment and 53 were part of the control group. There was no association between oropharyngeal colostrum immunotherapy and length of stay for preterm newborns with very low birth weight in the general sample. However, after stratification, a shorter hospital stay (42 versus 51 days, HR= 1.78, CI95%=1.02-3.09, p=0.04) was demonstrated among premature infants with ≥28 gestational weeks undergoing oropharyngeal colostrum immunotherapy. Conclusions: we found an association between oropharyngeal colostrum immunotherapy and shorter median length of hospital stay in the subgroup of premature infants ≥ 28 weeks of gestational age, but we did not find significant differences in those <28 weeks.

Resumo Objetivos: avaliar o efeito da imunoterapia orofaríngea de colostro no tempo de internamento hospitalar de recém-nascidos pré-termos de muito baixo peso. Métodos: estudo de intervenção, ambispectivo, que consistiu em oito administrações diárias de 0,2 ml (quatro gotas) de colostro, totalizando até 56 seringas (por até sete dias). O controle foi histórico. A variável independente principal: tempo de permanência hospitalar (dias). Realizada análise de sobrevivência pelo Método de Kaplan-Meier e estimado o efeito da sobrevida - Teste de Log Rank (Mantel-Cox) e Teste de Breslow (Wilcoxon Generalizado). Adotou-se o nível de significância de 5%. Resultados: das 109 duplas mães/filho, 56 fizeram parte da análise do grupo tratamento e 53 do controle. Não houve associação entre imunoterapia orofaríngea de colostro e tempo de internamento de recém-nascidos pré-termos de muito baixo peso na amostra geral. Após estratificação demonstrou-se menor tempo de permanência hospitalar (43 versus 51 dias, HR=1,78, IC95%= 1,02-3,09, p=0,04) entre os prematuros com ≥28 semanas gestacionais submetidos a imunoterapia orofaríngea de colostro. Conclusões: encontrou-se associação entre imunoterapia orofaríngea de colostro e menor mediana de tempo de internamento hospitalar no subgrupo de prematuros ≥ 28 semanas de idade gestacional, porém não foram encontradas diferenças significativas naqueles < 28 semanas.

Abstract Background: The newborn’s intestinal microbiota can vary with gestational age. Reliable analyses of stool samples require establishing the steps of collection, packaging, transportation, and storage. Objective: To describe the development of a protocol and test an algorithm for the sequence of actions and procedures for the collection, packaging, transportation, and storage of stool samples from Preterm Newborn (PTNB) admitted to a Neonatal Intensive Care Unit (NICU). Materials and methods: This is a descriptive, observational study linked to a non-randomized controlled clinical trial conducted in the NICU of two public hospitals covenanted to the Unified Health System of a large city in inland northeastern Brazil. The methodology adopted in its development followed the Brazilian Ministry of Health Preparation Guide recommendations. Results: The flow of actions and procedures was consistent with scientific evidence. The sequence of work process steps to implement the proposed protocol was tested and resulted in a graphical representation of an algorithm compatible with the reality of Brazilian public hospitals. Conclusion: Following the protocol steps with the description of the rules of conduct and recommendations regarding the collection, packaging, transportation, and storage of stool samples from PTNBs ensured the preservation and integrity of the bacterial DNA in the stools sample. The details of the recommendations will allow their reproducibility and improvement by professionals and researchers with similar study objects. Trial registration: World Health Organization (WHO) under Universal Trial Number (UTN) code U1111–1266–2295, under register RBR-3mm7cs in the Brazilian Registry of Clinical Trials (REBEC).

ABSTRACT OBJECTIVE To investigated the effect of oropharyngeal colostrum immunotherapy in reducing the time required for very low birth weight preterm newborns (VLBW-PTNB: < 1,500g and < 37 weeks) to achieve full enteral nutrition. METHODS Literature search was conducted using four databases, including gray literature, with additional manual search of the references of selected articles. Eligibility criteria consisted of randomized clinical trials, without restriction regarding the date or language of the publication. Two independent reviewers performed the article selection and data extraction. The random-effects meta-analysis used a non-standard technique to assess the mean difference in days to achieve full enteral nutrition, carried out by the Stata 15 statistic program. RESULTS The systematic review comprised 10 studies, and five were selected for meta-analysis, with a population of 764 VLBW-PTNB and gestational age of birth between 25 and 32 weeks. The studies were conducted between 2011 and 2018 in North America, Asia and Africa, with only one conducted in South America. Altogether, they reported the number of days it took 708 VLBW-PTNB to achieve full enteral nutrition, with newborns treated with immunotherapy showing a shorter time in only three studies. Meta-analysis showed a mean difference of -4.26 days, (95% CI -7.44; -1.08d), with high heterogeneity (I2 = 83.1%). CONCLUSION The use of oropharyngeal colostrum immunotherapy can reduce the time for VLBW-PTNB to achieve full nutrition when compared to those who used a placebo or received routine care.

Resumo O objetivo do estudo foi averiguar os fatores associados à leitura da Caderneta de Saúde da Criança (CSC) pelas mães e a seu preenchimento pelos profissionais de saúde, em Feira de Santana (Bahia), em 2009. Estudo transversal com aplicação de 727 formulários às mães de crianças menores de um ano. Os desfechos estudados foram: leitura materna da caderneta e preenchimento de medidas de peso e comprimento, por profissionais de saúde. Realizou-se análise de regressão logística com valor de p ≤ 5%. A prevalência de leitura, medidas de peso e comprimento foram, respectivamente, 8,1%, 68,9% e 47,3%. Maior escolaridade materna associou-se a maiores chances de leitura. Registros de peso e comprimento foram mais prevalentes em mães com idade < 35 anos. Realizar puericultura em áreas do Programa de Saúde da Família ou dos Agentes Comunitários de Saúde associou-se positivamente com anotações do comprimento, apesar das baixas prevalências dos registros. Idade da criança ≥ 6 meses associou-se a todos os desfechos. Demonstrou-se a subutilização da CSC por mães e profissionais de saúde, indicativos da necessidade de medidas educativas com treinamentos dos profissionais de saúde e orientações às mães sobre a importância e o manejo da mesma.

Abstract This study aimed to investigate the factors associated with mothers reading the Child Health Handbook (CHH) and health professionals completing this instrument, in Feira de Santana, Bahia, 2009. This is a cross-sectional study with the application of 727 forms to mothers of children under one year of age. The outcomes studied were mother reading the CHH and health professionals completing weight and height measures. We performed a logistic regression analysis with p ≤ 0.05. The prevalence of reading, weight and height were, respectively, 81.1%, 68.9% and 47.3%. Mothers with a higher level of education had a greater chance of reading the CHH. Recording weight and height was more prevalent in mothers who were under 35 years of age. Performing childcare in areas of the Family Health Program or the Community Health Workers was positively associated with the height’s record, despite the low prevalence of records. Child age greater than six months was positively associated with all outcomes. It demonstrated the underutilization of CHH by mothers and health professionals, which indicates the need for training of health professionals and guidance to mothers on the importance and management of this issue.

Resumo O presente estudo tem como objetivo identificar os fatores associados à intenção materna para amamentar (IMA) em gestantes. Foi realizada revisão sistemática de estudos de bases de dados Medical Literature Analysis and Retrieval System Online (PubMed/Medline) e Literatura Latino-Americana e do Caribe em Ciências da Saúde (Lilacs). Dois revisores independentes buscaram artigos, com os “Medical Subject Headings” e os “Descritores em Ciência e Saúde”, referentes: ao aleitamento materno, à intenção e às gestantes. Foram incluídas pesquisas que apresentavam IMA como desfecho, em inglês, português e espanhol; e classificadas, quanto à qualidade metodológica, de acordo com critérios de validade interna e externa de uma pesquisa, com pontuação máxima de 30 pontos. Selecionou-se nove artigos, com qualificação metodológica entre 12 e 21 pontos, que investigaram 68 variáveis; 35 dessas se associaram à IMA. As características associadas à IMA em gestantes, relatadas em dois ou mais estudos, foram: primeira gestação, maior escolaridade e idade materna, experiência prévia com a amamentação, ausência do hábito de fumar e residir com o companheiro. O conhecimento das características associadas à IMA representa um passo fundamental no delineamento de ações que visem maior duração do aleitamento materno.

Abstract This study seeks to identify the factors associated with maternal intention to breastfeed (MIB) in pregnant women. A systematic review of articles in the Medical Literature Analysis and Retrieval System Online (PubMed/Medline) and the Latin American and Caribbean Literature in Health Sciences (Lilacs) databases was conducted. Two independent reviewers performed the search, using the “Medical Subject Headings” and “Key words in Science and Health” vocabulary, referring to: breastfeeding, intention, and pregnant women. Research articles in English, Portuguese and Spanish that analyzed MIB as an outcome were selected. The studies were classified by methodological quality, using a scale that considered internal and external validity criteria, with a maximum score of 30 points. Nine articles were selected, with a methodological quality varying from 12 to 21 points. The studies assessed 68 variables; 35 of which were associated with MIB. The characteristics associated with MIB found in two or more studies were: primiparity, higher level of schooling and older maternal age, prior experience with breastfeeding, not smoking, and living with a partner. Knowledge of the characteristics associated with MIB represents a fundamental step in the planning of actions that seek to increase the duration of breastfeeding.