ABSTRACT Horseweed (Conyza sumatrensis), the major weed already identified in southern Brazil come presenting unsatisfactory control in response to application of herbicide chlorimuron-ethyl and glyphosate. In this context, the aim of this study was evaluating alternative herbicides for control of biotypes of C. sumatrensis with low-level resistance to the herbicide chlorimuron-ethyl and glyphosate-resistant. The experiment was carried out in a greenhouse, in a period during the interval April to August 2012, in Passo Fundo / RS. The experimental design was the completely randomized with four replications, with 15 herbicide treatments, plus an untreated control. The variables evaluated were percent control at 14, 21 and 28 days after treatment (DAT) and dry weight of shoot at 28 DAT. The results showed that the biotypes were 100% controlled at 28 DAT for alternative treatments of paraquat + diuron, ammonium glufosinate, glyphosate + 2,4-D, glyphosate and ammonium glufosinate; 2,4-D; tembotrione and tembotrione + atrazine. The biotype 17 showed less sensitivity to ALS-inhibiting herbicides, and biotypes 05, 17 and 20 were not controlled by glyphosate.
RESUMO A buva (Conyza sumatrensis), uma das principais plantas daninhas já identificadas no sul do Brasil, vem apresentando controle insatisfatório, em resposta à aplicação dos herbicidas chlorimuron-ethyl e glyphosate. Por esta razão, o objetivo deste estudo foi avaliar herbicidas alternativos, visando ao controle de biótipos de C. sumatrensis, com resistência de nível baixo ao herbicida chlorimuron-ethyl e resistentes ao herbicida glyphosate. O experimento foi realizado em casa de vegetação, entre abril e agosto de 2012, no município de Passo Fundo, RS. O delineamento experimental foi o inteiramente casualizado, com quatro repetições, sendo avaliados 15 tratamentos com herbicidas, além de uma testemunha sem aplicação. As variáveis consideradas foram controle percentual, aos 14, 21 e 28 dias após a aplicação dos tratamentos (DAT) e a matéria seca da parte aérea, aos 28 DAT. Como resultados, observou-se que os biótipos foram 100% controlados, aos 28 DAT, pelos tratamentos alternativos de paraquat + diuron; ammonium glufosinate; glyphosate + 2,4-D; glyphosate + ammonium glufosinate; 2,4-D; tembotrione e tembotrione + atrazine. O biótipo 17 evidenciou menor sensibilidade aos herbicidas inibidores da ALS e os biótipos 05, 17 e 20 não foram controlados pelo herbicida glyphosate.
Some of the most significant breakthroughs in the biological sciences this century will emerge from the development of next generation sequencing technologies. The ease of availability of DNA sequence made possible through these new technologies has given researchers opportunities to study organisms in a manner that was not possible with Sanger sequencing. Scientists will, therefore, need to embrace genomics, as well as develop and nurture the human capacity to sequence genomes and utilise the 'tsunami' of data that emerge from genome sequencing. In response to these challenges, we sequenced the genome of Fusarium circinatum, a fungal pathogen of pine that causes pitch canker, a disease of great concern to the South African forestry industry. The sequencing work was conducted in South Africa, making F. circinatum the first eukaryotic organism for which the complete genome has been sequenced locally. Here we report on the process that was followed to sequence, assemble and perform a preliminary characterisation of the genome. Furthermore, details of the computer annotation and manual curation of this genome are presented. The F. circinatum genome was found to be nearly 44 million bases in size, which is similar to that of four other Fusarium genomes that have been sequenced elsewhere. The genome contains just over 15 000 open reading frames, which is less than that of the related species, Fusarium oxysporum, but more than that for Fusarium verticillioides. Amongst the various putative gene clusters identified in F. circinatum, those encoding the secondary metabolites fumosin and fusarin appeared to harbour evidence of gene translocation. It is anticipated that similar comparisons of other loci will provide insights into the genetic basis for pathogenicity of the pitch canker pathogen. Perhaps more importantly, this project has engaged a relatively large group of scientists including students in a significant genome project that is certain to provide a platform for growth in this important area of research in the future.
Simaba polyphylla is a small tree found in the Amazon region, known by the common names "marupazinho" or "serve para tudo". It is used in traditional medicine for the treatment of fevers. This work describes the phytochemical study of the hexane extract and chloroform fraction obtained by partitioning the methanol extract of stems, which led to isolation and identification of the triterpenes niloticin, dyhidroniloticin, taraxerone and of the cytotoxic alkaloid 9-methoxy-canthin-6-one. These compounds are described for the first time in S. polyphylla.
Objective. To assess accidental poisoning in children in the Caribbean country of Antigua and Barbuda, including the incidence, the types of substances ingested, the age of the children involved, and the clinical outcomes. The results from Antigua and Barbuda were compared with the results of other reports from the English-speaking Caribbean and from the United States of America. Design and Methods. We performed a retrospective review of the charts of all patients less than 13 years old admitted to the Children's Ward at Holberton Hospital in Antigua for accidental poisoning between March 1989 and March 1999. Those data were compared with data from earlier reports from Barbados, Guyana, Jamaica, and the United States of America. Results. In Antigua and Barbuda there were 255 hospital admissions for accidental poisoning among children below 13 years old over that 10-year period. Of the 255 ingestions, 115 of them (45%) were in 1-year-old children, 69 (27%) were in 2-year-old children, and 26 (10%) were in 3-year-old children. These proportions in Antigua and Barbuda are similar to the age patterns seen in the other countries with which we made comparisons. In Antigua and Barbuda there was an annual average of 26 hospital admissions for poisoning for the roughly 20 000 children below 13 years of age, for a rate of 1.3 per 1 000. In comparing the patterns of childhood poisoning in all the countries we studied, we found that, as economic levels rose, there was a shift in the substances ingested, with hydrocarbon and plant ingestions decreasing and chemical and medication ingestions increasing. Conclusions. There is an increasing variety and complexity of poisonous substances ingested as economic conditions improve. This trend would make the establishment of a poison control center for the English-speaking Caribbean a logical step.
Objetivos. Investigar las intoxicaciones accidentales de niños en Antigua y Barbuda desde el punto de vista de la incidencia, tipos de sustancias implicadas, edad de los niños y desenlaces clínicos. Los resultados obtenidos en este país se compararon con los de otros estudios realizados en diferentes países caribeños de habla inglesa y en los Estados Unidos de América (EE. UU.). Métodos. Se realizó una revisión retrospectiva de las historias clínicas de todos los pacientes de menos de 13 años de edad ingresados entre marzo de 1989 y marzo de 1999 en la planta de pediatría del Hospital Holberton de Antigua por intoxicación accidental. Los datos obtenidos se compararon con los comunicados en estudios anteriores realizados en Barbados, Guyana, Jamaica y EE. UU. Resultados. Durante este período de 10 años, hubo en Antigua y Barbuda 255 ingresos de niños de menos de 13 años por intoxicación accidental (media de 26 ingresos por año). En una población de aproximadamente 20 000 niños menores de 13 años, esto representa una proporción de 1,3 por 1 000. De estos 255 casos, 115 (45%) ocurrieron en niños de 1 año, 69 (27%) en niños de 2 años y 26 (10%) en niños de 3 años. Esta distribución por edades fue similar a la observada en los demás países. Al comparar los tipos de intoxicación en todos los países estudiados, se verificó que las sustancias implicadas cambiaban a medida que aumentaba el nivel económico: disminuían los hidrocarburos y las plantas y aumentaban los medicamentos y los productos químicos. Conclusiones. A medida que aumenta el nivel económico, también lo hace la complejidad de las sustancias implicadas. Esta tendencia debería llevar al establecimiento de un centro de control de intoxicaciones en los países caribeños de habla inglesa.