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Abstract Polyethylenimine (PEI) has been explored as an efficient non-viral system for delivering genes to cells; however, there were no protocols for its use in porcine fetal fibroblasts (PFF). Therefore, we compared different concentrations of FITC-PEI (0.625, 1.25, 2.5, 5, 10, 20, 40, or 80 µg/mL) and incubation times (30 min, 1 h, or 2 h). It was observed that the incubation time did not affect the internalization of the PEI-FITC and that 30 min was sufficient to capture the complex. The concentrations higher than 10 µg/mL could reach many marked PFF (>90%). Then, two PEI concentrations were tested, 10 or 40 µg/mL, combined with an N/P of 2 with the pmhyGENIE-5 for 30 min. The percentage of PFF-GFP positive was similar between the PEI concentrations in the evaluation time points (24 h, 48 h, and 72 h). However, 40 µg/mL caused higher membrane damage rates. Thus, it can be concluded that concentrations between 10 – 80 µg/ml of PEI promote high incorporation rates, even in periods as short as 30 minutes. Furthermore, it can be stated that the transfection condition used in Polyplexes 1 (10 µg/mL of PEI and 37.5 µg/mL of pmhyGENIE-5 for 30 min) efficiently produces genetically edited porcine fetal fibroblasts with low cell damage.

https://doi.org/10.1590/1984-3143-ar2024-0026

Protective effects of mesenchymal stromal cell-derived secretome on dermonecrosis induced in rabbits by Loxosceles intermedia spider venom
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Rodrigues, Gabriela Marques

; Almeida, Mara Elvira de

; Marcelino, Sóstenes Apolo Correia Fernandes, Paula Bretas Ullmann Cruz, Jessica Oliveira Pereira da Araújo, Françoise Louanne Ferreira, Raquel da Silva Botelho, Ana Flávia Machado Bedoya, Francisco Javier Cahuana, Gladys Margot Hitos, Ana Belén Soria, Bernat Costal-Oliveira, Fernanda Duarte, Clara Guerra Tejedo, Juan R. Chávez-Olórtegui, Carlos Melo, Marília Martins

Journal of Venomous Animals and Toxins including Tropical Diseases

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2024, Volume 30 elocation e20240004

Abstract: En | Text: En | PDF: En

Abstract Background: Loxoscelism refers to a set of clinical manifestations caused by the bite of spiders from the Loxosceles genus. The classic clinical symptoms are characterized by an intense inflammatory reaction at the bite site followed by local necrosis and can be classified as cutaneous loxoscelism. This cutaneous form presents difficult healing, and the proposed treatments are not specific or effective. This study aimed to evaluate the protective effect of mesenchymal stromal cells-derived secretome on dermonecrosis induced by Loxosceles intermedia spider venom in rabbits. Methods: Sixteen rabbits were distributed into four groups (n = 4). Except for group 1 (G1), which received only PBS, the other three groups (G2, G3, and G4) were initially challenged with 10 μg of L. intermedia venom, diluted in 100 μL of NaCl 0.9%, by intradermic injection in the interscapular region. Thirty minutes after the challenge all groups were treated with secretome, except for group 2. Group 1 (G1-control group) received intradermal injection (ID) of 60 μg of secretome in 0.15 M PBS; Group 2 (G2) received 0.9% NaCl via ID; Group 3 (G3) received 60 μg of secretome, via ID and Group 4 (G4), received 60 μg of secretome by intravenous route. Rabbits were evaluated daily and after 15 days were euthanized, necropsied and skin samples around the necrotic lesions were collected for histological analysis. Results: Rabbits of G1 did not present edema, erythema, hemorrhagic halo, or necrosis. In animals from G2, G3, and G4, edema appeared after 6h. However, minor edema was observed in the animals of G2 and G3. Hemorrhagic halo was observed in animals, six hours and three days after, on G2, G3, and G4. Macroscopically, in G4, only one animal out of four had a lesion that evolved into a dermonecrotic wound. No changes were observed in the skin of the animals of G1, by microscopic evaluation. All animals challenged with L. intermedia venom showed similar alterations, such as necrosis and heterophilic infiltration. However, animals from G4 showed fibroblast activation, early development of connective tissue, neovascularization, and tissue re-epithelialization, indicating a more prominent healing process. Conclusion: These results suggest that secretome from mesenchymal stromal cells cultured in a xeno-free and human component-free culture media can be promising to treat dermonecrosis caused after Loxosceles spiders bite envenoming.

https://doi.org/10.1590/1678-9199-jvatitd-2024-0004

Late peripheral facial paralysis after COVID-19: a rapid systematic review and two case reports
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Cavalcante, Thalitta Mendes

; Gubert, Vanessa Terezinha

; Lima, Carolina de Deus Luciano, Larissa Anjos Croda, Mariana Garcia Venturini, James Gasparoto, Antonio Luiz Dal Bello Santiago, Wellyngton Matheus Souza Motta-Castro, Ana Rita Coimbra Reis, Fernanda Paes Marques, Ana Paula da Costa Lorenz, Aline Pedroso Fava, Wellington Santos Zardin, Marina Castilhos Souza Umaki Chaves, Cláudia Elizabeth Volpe Braga, Gabriel Pereira Paniago, Anamaria Mello Miranda Oliveira, Sandra Maria do Valle Leone de

Journal of Venomous Animals and Toxins including Tropical Diseases

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2022, Volume 28 elocation e20220020

Abstract: En | Text: En | PDF: En

Abstract Peripheral facial paralysis (PFP) has been shown to be a neurological manifestation of COVID-19. The current study presents two cases of PFP after COVID-19, along with a rapid review of known cases in the literature. Both case reports were conducted following CARE guidelines. We also performed a systematic review of PFP cases temporally related to COVID-19 using PubMed, Embase, and Cochrane Library databases on August 30, 2021, using a rapid review methodology. The two patients experienced PFP 102 and 110 days after COVID-19 symptom onset. SARS-CoV-2 RNA was detected in nasal samples through reverse-transcription real-time polymerase chain reaction (RT-qPCR) testing. Anosmia was the only other neurological manifestation. PFP was treated with steroids in both cases, with complete subsequent recovery. In the rapid review, we identified 764 articles and included 43 studies. From those, 128 patients with PFP were analyzed, of whom 42.1% (54/128) were male, 39.06% (50/128) female, and in 23 cases the gender was not reported. The age range was 18 to 59 (54.68%). The median time between COVID-19 and PFP was three days (ranging from the first symptom of COVID-19 to 40 days after the acute phase of infection). Late PFP associated with COVID-19 presents mild symptoms and improves with time, with no identified predictors. Late PFP should be added to the spectrum of neurological manifestations associated with the long-term effects of SARS-CoV-2 infection as a post COVID-19 condition.

https://doi.org/10.1590/1678-9199-jvatitd-2022-0020

Práticas de ressuscitação volêmica em unidades de terapia intensiva brasileiras: uma análise secundária do estudo Fluid-TRIPS
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Freitas, Flavio Geraldo Rezende de

; Hammond, Naomi

; Li, Yang

; Azevedo, Luciano Cesar Pontes de

; Cavalcanti, Alexandre Biasi

; Taniguchi, Leandro

; Gobatto, André

; Japiassú, André Miguel

; Bafi, Antonio Tonete

; Mazza, Bruno Franco

; Noritomi, Danilo Teixeira Dal-Pizzol, Felipe Bozza, Fernando Salluh, Jorge Ibrahin Figueira Westphal, Glauco Adrieno Soares, Márcio Assunção, Murillo Santucci César de Lisboa, Thiago Lobo, Suzana Margarete Ajeje Barbosa, Achilles Rohlfs Ventura, Adriana Fonseca Souza, Ailson Faria de Silva, Alexandre Francisco Toledo, Alexandre Reis, Aline Cembranel, Allan Rea Neto, Alvaro Gut, Ana Lúcia Justo, Ana Patricia Pierre Santos, Ana Paula Albuquerque, André Campos D. de Scazufka, André Rodrigues, Antonio Babo Fernandino, Bruno Bonaccorsi Silva, Bruno Goncalves Vidal, Bruno Sarno Pinheiro, Bruno Valle Pinto, Bruno Vilela Costa Feijo, Carlos Augusto Ramos Abreu Filho, Carlos de Bosso, Carlos Eduardo da Costa Nunes Moreira, Carlos Eduardo Nassif Ramos, Carlos Henrique Ferreira Tavares, Carmen Arantes, Cidamaiá Grion, Cintia Mendes, Ciro Leite Kmohan, Claudio Piras, Claudio Castro, Cristine Pilati Pileggi Lins, Cyntia Beraldo, Daniel Fontes, Daniel Boni, Daniela Castiglioni, Débora Paisani, Denise de Moraes Pedroso, Durval Ferreira Fonseca Mattos, Ederson Roberto Brito Sobrinho, Edgar de Troncoso, Edgar M. V. Rodrigues Filho, Edison Moraes Nogueira, Eduardo Enrico Ferrari Ferreira, Eduardo Leme Pacheco, Eduardo Souza Jodar, Euzebio Ferreira, Evandro L. A. Araujo, Fabiana Fernandes de Trevisol, Fabiana Schuelter Amorim, Fábio Ferreira Giannini, Fabio Poianas Santos, Fabrício Primitivo Matos Buarque, Fátima Lima, Felipe Gallego Costa, Fernando Antonio Alvares da Sad, Fernando Cesar dos Anjos Aranha, Fernando G. Ganem, Fernando Callil, Flavio Costa Filho, Francisco Flávio Dall´Arto, Frederico Toledo Campo Moreno, Geovani Friedman, Gilberto Moralez, Giulliana Martines Silva, Guilherme Abdalla da Costa, Guilherme Cavalcanti, Guilherme Silva Cavalcanti, Guilherme Silva Betônico, Gustavo Navarro Betônico, Gustavo Navarro Reis, Hélder Araujo, Helia Beatriz N. Hortiz Júnior, Helio Anjos Guimaraes, Helio Penna Urbano, Hugo Maia, Israel Santiago Filho, Ivan Lopes Farhat Júnior, Jamil Alvarez, Janu Rangel Passos, Joel Tavares Paranhos, Jorge Eduardo da Rocha Marques, José Aurelio Moreira Filho, José Gonçalves Andrade, Jose Neto Sobrinho, José Onofre de C Bezerra, Jose Terceiro de Paiva Alves, Juliana Apolônio Ferreira, Juliana Gomes, Jussara Sato, Karina Midori Gerent, Karine Teixeira, Kathia Margarida Costa Conde, Katia Aparecida Pessoa Martins, Laércia Ferreira Figueirêdo, Lanese Rezegue, Leila Tcherniacovsk, Leonardo Ferraz, Leone Oliveira Cavalcante, Liane Rabelo, Ligia Miilher, Lilian Garcia, Lisiane Tannous, Luana Hajjar, Ludhmila Abrahão Paciência, Luís Eduardo Miranda Cruz Neto, Luiz Monteiro da Bley, Macia Valeria Sousa, Marcelo Ferreira Puga, Marcelo Lourencini Romano, Marcelo Luz Pereira Nobrega, Marciano Arbex, Marcio Rodrigues, Márcio Leite Guerreiro, Márcio Osório Rocha, Marcone Alves, Maria Angela Pangoni Alves, Maria Angela Pangoni Rosa, Maria Doroti Dias, Mariza D’Agostino Martins, Miquéias Oliveira, Mirella de Moretti, Miriane Melo Silveira Matsui, Mirna Messender, Octavio Santarém, Orlando Luís de Andrade Silveira, Patricio Júnior Henrique da Vassallo, Paula Frizera Antoniazzi, Paulo Gottardo, Paulo César Correia, Paulo Ferreira, Paulo Torres, Paulo Silva, Pedro Gabrile M. de Barros e Foernges, Rafael Gomes, Rafael Moraes, Rafael Nonato filho, Raimundo Borba, Renato Luis Gomes, Renato V Cordioli, Ricardo Lima, Ricardo López, Ricardo Pérez Gargioni, Ricardo Rath de Oliveira Rosenblat, Richard Souza, Roberta Machado de Almeida, Roberto Narciso, Roberto Camargo Marco, Roberto waltrick, Roberto Biondi, Rodrigo Figueiredo, Rodrigo Dutra, Rodrigo Santana Batista, Roseane Felipe, Rouge Franco, Rubens Sergio da Silva Houly, Sandra Faria, Sara Socorro Pinto, Sergio Felix Luzzi, Sergio Sant’ana, Sergio Fernandes, Sergio Sonego Yamada, Sérgio Zajac, Sérgio Vaz, Sidiner Mesquita Bezerra, Silvia Aparecida Bezerra Farhat, Tatiana Bueno Tardivo Santos, Thiago Martins Smith, Tiago Silva, Ulysses V. A. Damasceno, Valnei Bento Nobre, Vandack Dantas, Vicente Cés de Souza Irineu, Vivian Menezes Bogado, Viviane Nedel, Wagner Campos Filho, Walther Dantas, Weidson Viana, William Oliveira Filho, Wilson de Delgadinho, Wilson Martins Finfer, Simon Machado, Flavia Ribeiro

Revista Brasileira de Terapia Intensiva

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apr 2021, Volume 33 N. 2 Pages 206 - 218

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Descrever as práticas de ressuscitação volêmica em unidades de terapia intensiva brasileiras e compará-las com as de outros países participantes do estudo Fluid-TRIPS. Métodos: Este foi um estudo observacional transversal, prospectivo e internacional, de uma amostra de conveniência de unidades de terapia intensiva de 27 países (inclusive o Brasil), com utilização da base de dados Fluid-TRIPS compilada em 2014. Descrevemos os padrões de ressuscitação volêmica utilizados no Brasil em comparação com os de outros países e identificamos os fatores associados com a escolha dos fluidos. Resultados: No dia do estudo, foram incluídos 3.214 pacientes do Brasil e 3.493 pacientes de outros países, dos quais, respectivamente, 16,1% e 26,8% (p < 0,001) receberam fluidos. A principal indicação para ressuscitação volêmica foi comprometimento da perfusão e/ou baixo débito cardíaco (Brasil 71,7% versus outros países 56,4%; p < 0,001). No Brasil, a percentagem de pacientes que receberam soluções cristaloides foi mais elevada (97,7% versus 76,8%; p < 0,001), e solução de cloreto de sódio a 0,9% foi o cristaloide mais comumente utilizado (62,5% versus 27,1%; p < 0,001). A análise multivariada sugeriu que os níveis de albumina se associaram com o uso tanto de cristaloides quanto de coloides, enquanto o tipo de prescritor dos fluidos se associou apenas com o uso de cristaloides. Conclusão: Nossos resultados sugerem que cristaloides são usados mais frequentemente do que coloides para ressuscitação no Brasil, e essa discrepância, em termos de frequências, é mais elevada do que em outros países. A solução de cloreto de sódio 0,9% foi o cristaloide mais frequentemente prescrito. Os níveis de albumina sérica e o tipo de prescritor de fluidos foram os fatores associados com a escolha de cristaloides ou coloides para a prescrição de fluidos.

Abstract Objective: To describe fluid resuscitation practices in Brazilian intensive care units and to compare them with those of other countries participating in the Fluid-TRIPS. Methods: This was a prospective, international, cross-sectional, observational study in a convenience sample of intensive care units in 27 countries (including Brazil) using the Fluid-TRIPS database compiled in 2014. We described the patterns of fluid resuscitation use in Brazil compared with those in other countries and identified the factors associated with fluid choice. Results: On the study day, 3,214 patients in Brazil and 3,493 patients in other countries were included, of whom 16.1% and 26.8% (p < 0.001) received fluids, respectively. The main indication for fluid resuscitation was impaired perfusion and/or low cardiac output (Brazil: 71.7% versus other countries: 56.4%, p < 0.001). In Brazil, the percentage of patients receiving crystalloid solutions was higher (97.7% versus 76.8%, p < 0.001), and 0.9% sodium chloride was the most commonly used crystalloid (62.5% versus 27.1%, p < 0.001). The multivariable analysis suggested that the albumin levels were associated with the use of both crystalloids and colloids, whereas the type of fluid prescriber was associated with crystalloid use only. Conclusion: Our results suggest that crystalloids are more frequently used than colloids for fluid resuscitation in Brazil, and this discrepancy in frequencies is higher than that in other countries. Sodium chloride (0.9%) was the crystalloid most commonly prescribed. Serum albumin levels and the type of fluid prescriber were the factors associated with the choice of crystalloids or colloids for fluid resuscitation.

https://doi.org/10.5935/0103-507x.20210028 273 downloads

Differences in children and adolescents with SARS-CoV-2 infection: a cohort study in a Brazilian tertiary referral hospital
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Marques, Heloisa Helena de Sousa

; Pereira, Maria Fernanda Badue

; Santos, Angélica Carreira dos

; Fink, Thais Toledo

; Paula, Camila Sanson Yoshino de

; Litvinov, Nadia

; Schvartsman, Claudio

; Delgado, Artur Figueiredo

; Gibelli, Maria Augusta Bento Cicaroni

; Carvalho, Werther Brunow de

; Odone Filho, Vicente

; Tannuri, Uenis

; Carneiro-Sampaio, Magda

; Grisi, Sandra

; Duarte, Alberto José da Silva

; Antonangelo, Leila

; Francisco, Rossana Pucineli Vieira

; Okay, Thelma Suely

; Batisttella, Linamara Rizzo

; Carvalho, Carlos Roberto Ribeiro de

; Brentani, Alexandra Valéria Maria

; Silva, Clovis Artur

; Eisencraft, Adriana Pasmanik Rossi Junior, Alfio Fante, Alice Lima Cora, Aline Pivetta Reis, Amelia Gorete A. de Costa Ferrer, Ana Paula Scoleze Andrade, Anarella Penha Meirelles de Watanabe, Andreia Gonçalves, Angelina Maria Freire Waetge, Aurora Rosaria Pagliara Silva, Camila Altenfelder Ceneviva, Carina Lazari, Carolina dos Santos Abellan, Deipara Monteiro Santos, Emilly Henrique dos Sabino, Ester Cerdeira Bianchini, Fabíola Roberta Marim Alcantara, Flávio Ferraz de Paes Ramos, Gabriel Frizzo Leal, Gabriela Nunes Rodriguez, Isadora Souza Pinho, João Renato Rebello Carneiro, Jorge David Avaizoglou Paz, Jose Albino Ferreira, Juliana Carvalho Ferranti, Juliana Ferreira Ferreira, Juliana de Oliveira Achili Framil, Juliana Valéria de Souza Silva, Katia Regina da Kanunfre, Kelly Aparecida Bastos, Karina Lucio de Medeiros Galleti, Karine Vusberg Cristofani, Lilian Maria Suzuki, Lisa Campos, Lucia Maria Arruda Perondi, Maria Beatriz de Moliterno Diniz, Maria de Fatima Rodrigues Fonseca, Maria Fernanda Mota Cordon, Mariana Nutti de Almeida Pissolato, Mariana Peres, Marina Silva Garanito, Marlene Pereira Imamura, Marta Dorna, Mayra de Barros Luglio, Michele Rocha, Mussya Cisotto Aikawa, Nadia Emi Degaspare, Natalia Viu Sakita, Neusa Keico Udsen, Nicole Lee Scudeller, Paula Gobi Gaiolla, Paula Vieira de Vincenzi Severini, Rafael da Silva Giannasi Rodrigues, Regina Maria Toma, Ricardo Katsuya Paula, Ricardo Iunis Citrangulo de Palmeira, Patricia Forsait, Silvana Farhat, Sylvia Costa Lima Sakano, Tânia Miyuki Shimoda Koch, Vera Hermina Kalika Cobello Junior, Vilson

Clinics

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2021, Volume 76 elocation e3488

Abstract: En | Text: En | PDF: En

OBJECTIVES: To compare demographic/clinical/laboratory/treatments and outcomes among children and adolescents with laboratory-confirmed coronavirus disease 2019 (COVID-19). METHODS: This was a cross-sectional study that included patients diagnosed with pediatric COVID-19 (aged <18 years) between April 11, 2020 and April 22, 2021. During this period, 102/5,951 (1.7%) of all admissions occurred in neonates, children, and adolescents. Furthermore, 3,962 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection samples were processed in patients aged <18 years, and laboratory-confirmed COVID-19 occurred in 155 (4%) inpatients and outpatients. Six/155 pediatric patients were excluded from the study. Therefore, the final group included 149 children and adolescents (n=97 inpatients and 52 outpatients) with positive SARS-CoV-2 results. RESULTS: The frequencies of sore throat, anosmia, dysgeusia, headache, myalgia, nausea, lymphopenia, pre-existing chronic conditions, immunosuppressive conditions, and autoimmune diseases were significantly reduced in children and adolescents (p<0.05). Likewise, the frequencies of enoxaparin use (p=0.037), current immunosuppressant use (p=0.008), vasoactive agents (p=0.045), arterial hypotension (p<0.001), and shock (p=0.024) were significantly lower in children than in adolescents. Logistic regression analysis showed that adolescents with laboratory-confirmed COVID-19 had increased odds ratios (ORs) for sore throat (OR 13.054; 95% confidence interval [CI] 2.750-61.977; p=0.001), nausea (OR 8.875; 95% CI 1.660-47.446; p=0.011), and lymphopenia (OR 3.575; 95% CI 1.355-9.430; p=0.010), but also had less hospitalizations (OR 0.355; 95% CI 0.138-0.916; p=0.032). The additional logistic regression analysis on patients with preexisting chronic conditions (n=108) showed that death as an outcome was significantly associated with pediatric severe acute respiratory syndrome (SARS) (OR 22.300; 95% CI 2.341-212.421; p=0.007) and multisystem inflammatory syndrome in children (MIS-C) (OR 11.261; 95% CI 1.189-106. 581; p=0.035). CONCLUSIONS: Half of the laboratory-confirmed COVID-19 cases occurred in adolescents. Individuals belonging to this age group had an acute systemic involvement of SARS-CoV-2 infection. Pediatric SARS and MIS-C were the most important factors associated with the mortality rate in pediatric chronic conditions with COVID-19.

https://doi.org/10.6061/clinics/2021/e3488

Características epidemiológicas da paralisia cerebral em crianças e adolescentes em uma capital do nordeste brasileiro
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Peixoto, Marcus Valerius da Silva

; Duque, Andrezza Marques

; Carvalho, Susana de

; Gonçalves, Társilla Pereira

; Novais, Ana Paula de Souza

; Nunes, Marco Antônio Prado

Fisioterapia e Pesquisa

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dec 2020, Volume 27 N. 4 Pages 405 - 412

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO O objetivo deste estudo foi descrever a prevalência de paralisia cerebral entre crianças e adolescentes, seus subtipos, as possíveis comorbidades e as características socioeconômicas das famílias. Foi realizado um estudo epidemiológico do tipo transversal a partir de um inquérito de base populacional sobre a paralisia cerebral em crianças e adolescentes na cidade de Aracaju (SE), Brasil. O estudo obteve informações sobre 240 crianças e adolescentes com paralisia cerebral a partir das respostas a um questionário feitas por seus responsáveis. Foi encontrada a prevalência de período de 1,37 em cada mil. Alguns bairros possuem prevalência de três a quatro vezes maior, revelando que a taxa de prevalência total não é um indicador homogêneo. A maioria dos participantes foi do sexo masculino (56,25%), de raça/cor declarada como parda ou preta (67,50%), sendo que a média de idade foi de 8,56 anos. A paralisia cerebral de tipo espástica bilateral foi a mais frequente (45,42%) e a comorbidade referida na maioria dos casos foi a epilepsia (48,33%). A renda familiar mensal correspondia a $252,87 dólares. O estudo revelou que as crianças e adolescentes com paralisia cerebral são, em grande parte, pertencentes a minorias sociais, de raça/cor parda ou preta, e suas famílias vivem na linha da extrema pobreza.

ABSTRACT This study aimed to describe the prevalence of cerebral palsy among children and adolescents, its subtypes, associated comorbidities, and socioeconomic characteristics of families. This is a cross-sectional epidemiological study conducted from a population-based survey on cerebral palsy among children and adolescents in the city of Aracaju, Sergipe, Brazil. Results: Based on guardians’ answers to the proposed questionnaire, we obtained data on 240 children and adolescents with cerebral palsy in Aracaju. The period prevalence of cerebral palsy was 1.37 per 1,000 people. Some neighborhoods showed a prevalence three to four times higher than that found to the overall city, indicating that the total prevalence is not a homogeneous indicator. Most participants were male (56.25%), self-declared as mixed-race or Black (67.50%), and aged 8.56 years on average. Bilateral spastic was the most prevalent cerebral palsy subtype (45.42%), and epilepsy was the most common comorbidity (48.33%) along with intellectual disability. Household monthly income was $ 252.87. Our results indicate that children and adolescents with cerebral palsy are mostly from minoritized groups, Black or mixed-race, and live in extreme poverty.

RESUMEN El objetivo de este estudio fue describir la prevalencia de parálisis cerebral en niños y adolescentes, sus subtipos, las posibles comorbilidades y las características socioeconómicas de las familias. Se realizó un estudio epidemiológico transversal a partir de una encuesta poblacional sobre parálisis cerebral en niños y adolescentes de la ciudad de Aracaju, Brasil. El estudio obtuvo información de 240 niños y adolescentes con parálisis cerebral con base en las respuestas de sus padres a un cuestionario. Se encontró la prevalencia de período de 1,37 por mil. Algunos barrios tienen una prevalencia de tres a cuatro veces mayor, lo que revela que la tasa de prevalencia total no es un indicador homogéneo. La mayoría de los participantes eran varones (56,25%), de raza/color declarado parda o negra (67,50%), con un promedio de edad de 8,56 años. La parálisis cerebral espástica bilateral fue la más frecuente (45,42%), y la comorbilidad reportada en la mayoría de los casos fue la epilepsia (48,33%). El ingreso familiar mensual correspondió a 252,87 dólares. El estudio reveló que los niños y adolescentes con parálisis cerebral son mayoritariamente de minorías sociales, raza/color parda o negra, y sus familias viven en extrema pobreza.

https://doi.org/10.1590/1809-2950/20012527042020 1270 downloads

INFLAMMATORY BOWEL DISEASE CARE IN BRAZIL: HOW IT IS PERFORMED, OBSTACLES AND DEMANDS FROM THE PHYSICIANS’ PERSPECTIVE
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VILELA, Eduardo Garcia

; ROCHA, Henrique Carvalho

; MORAES, Antônio Carlos

; SANTANA, Genoile Oliveira

; PARENTE, José Miguel

; SASSAKI, Ligia Yukie

; MISZPUTEN, Sender Jenkiel

; QUARESMA, Abel Botelho

; CLARA, Ana Paula Hamer Sousa

; JESUS, Adélia Carmen Silva de

; PINTO, Arlene dos Santos

; SILVA, Bianca Loyo Pona Schiavetti da

; SILVA, Bruno César da

; FREIRE, Caio César Furtado

; SANTOS, Carlos Henrique Marques dos

; BRITO, Carlos

; TEIXEIRA, Ellen Francioni Lima

; MIRANDA, Eron Fabio

; ZABOT, Gilmara Pandolfo

; DUARTE, Jozelda Lemos

; LUDVIG, Juliano Coelho

; CAMPOS-LOBATO, Luiz Felipe de

; CASSOL, Ornella Sari

; SOUZA, Mardem Machado de

; ALMEIDA, Neogélia Pereira de

; PARRA, Rogério Serafim

; LIMA JÚNIOR, Sérgio Figueiredo de

; SAAD-HOSSNE, Rogério

Arquivos de Gastroenterologia

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dec 2020, Volume 57 N. 4 Pages 416 - 427

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO CONTEXTO: As doenças inflamatórias intestinais (DII) são afecções inflamatórias crônicas de caráter recorrente, cujas taxas de incidência e prevalência têm aumentado, inclusive no Brasil. A longo prazo, são responsáveis por danos estruturais que impactam na qualidade de vida, morbidade e mortalidade dos pacientes. OBJETIVO: Avaliar o perfil dos médicos que atendem pacientes com DII, assim como as características deste atendimento, demandas não atendidas e dificuldades. MÉTODOS: Um questionário contendo 17 variáveis foi elaborado e enviado para médicos, selecionados a partir do cadastro da Comissão das Estaduais do Grupo de Estudos da Doença Inflamatória Intestinal do Brasil (GEDIIB), totalizando 286 médicos de 101 cidades brasileiras distribuídas por 21 estados e Distrito Federal. RESULTADOS: A maioria dos médicos que respondeu o questionário foram Gastroenterologistas e Coloproctologistas. Mais de 60% tinham até 20 anos de atuação na especialidade e 53,14% trabalhavam em três locais ou mais. A dificuldade no acesso ou liberação de medicamentos ficou evidenciada neste questionário, assim como a dificuldade no encaminhamento para profissionais não médicos que atuam em DII. Mais de 75% dos médicos relataram dificuldades para realização de enteroscopia por duplo balão e cápsula endoscópica, e 67,8% para realização da calprotectina. Em relação ao número de pacientes atendidos por cada médico, foi evidenciado que não há uma concentração de pacientes sob a responsabilidade de poucos médicos. O infliximabe e o adalimumabe foram os biológicos mais utilizados e ficou evidenciada prescrição maior de derivados de 5-ASA para retocolite ulcerativa quando comparada à doença de Crohn. Os corticoides foram prescritos para uma parcela menor de pacientes em ambas doenças. Os temas “falha a terapia biológica” e “novas drogas” foram referidos como aqueles com maior prioridade para discussão em eventos científicos. Em relação às possíveis diferenças entre cada região e o restante do país, os médicos da região Norte relataram maior dificuldade no acesso a exames complementares e os médicos da região Nordeste, maior dificuldade no acesso ou liberação de medicamentos. CONCLUSÃO: Os dados obtidos por meio deste estudo mostram o perfil do atendimento médico especializado em DII e podem se constituir em ferramenta útil para para elaboração de políticas governamentais e para sociedade brasileira como um todo.

ABSTRACT BACKGROUND: Inflammatory bowel diseases (IBD) are chronic inflammatory affections of recurrent nature whose incidence and prevalence rates have increased, including in Brazil. In long term, they are responsible for structural damage that impacts quality of life, morbidity and mortality of patients. OBJECTIVE: To describe the profile of physicians who treat IBD patients as well as the characteristics of IBD care, unmet demands and difficulties. METHODS: A questionnaire containing 17 items was prepared and sent to 286 physicians from 101 Brazilian cities across 21 states and the Federal District, selected from the register of the State Commission of the “Study Group of Inflammatory Bowel Disease of Brazil” (GEDIIB). RESULTS: The majority of the physicians who answered the questionnaire were gastroenterologists and colorectal surgeons. More than 60% had up to 20 years of experience in the specialty and 53.14% worked at three or more locations. Difficulties in accessing or releasing medicines were evident in this questionnaire, as was referrals to allied healthy professionals working in IBD-related fields. More than 75% of physicians reported difficulties in performing double-balloon enteroscopy and capsule endoscopy, and 67.8% reported difficulties in measuring calprotectin. With regard to the number of patients seen by each physician, it was shown that patients do not concentrate under the responsibility of few doctors. Infliximab and adalimumab were the most commonly used biological medicines and there was a higher prescription of 5-ASA derivatives for ulcerative colitis than for Crohn’s disease. Steroids were prescribed to a smaller proportion of patients in both diseases. The topics “biological therapy failure” and “new drugs” were reported as those with higher priority for discussion in medical congresses. In relation to possible differences among the country’s regions, physicians from the North region reported greater difficulty in accessing complementary exams while those from the Northeast region indicated greater difficulty in accessing or releasing medicines. CONCLUSION: The data obtained through this study demonstrate the profile of specialized medical care in IBD and are a useful tool for the implementation of government policies and for the Brazilian society as a whole.

https://doi.org/10.1590/s0004-2803.202000000-77 1133 downloads

Tradução, adaptação transcultural e avaliação psicométrica da versão em português (brasileiro) do 14-item Health Literacy Scale
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Batista, Marília Jesus

; Marques, Ana Carolina de Paula

; Silva Junior, Manoelito Ferreira

; Alencar, Gizelton Pereira

; Sousa, Maria da Luz Rosário de

Ciência & Saúde Coletiva

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jul 2020, Volume 25 N. 7 Pages 2847 - 2857

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Foram avaliadas as propriedades psicométricas da versão brasileira do 14-item Health Literacy Scale (HLS-14). No estudo metodológico com delineamento transversal realizou-se tradução, adaptação transcultural e avaliação das propriedades psicométricas. Depois de passar por comitê de especialistas, traduzido e adaptado, o instrumento foi pré-testado em 52 adultos, e aplicado em 143 adultos e idosos de Piracicaba-SP. A consistência interna foi avaliada através do coeficiente de correlação de Kendall e α de Cronbach (>0,70) e a análise fatorial confirmatória (AFC) por meio do teste de qui-quadrado, raiz quadrada média residual padronizada (SRMR), raiz da média dos quadrados dos erros de aproximação (RMSEA) (<0,05), índice de ajuste comparativo (CFI) (>0,95) e índice de Tucker-Lewis (TLI) (>0,95). A equivalência operacional apresentou concordância entre as questões, com exceção das questões 6, 8, 9 e 14. O coeficiente α de Cronbach foi 0,82. Houve ajuste razoável na AFC, CFI=0,886, TLI=0,86, RMSEA=0,085 (IC90%: 0,065-0,105), SRMR=0,071, qui-quadrado (74 graus de liberdade) =149,510, p<0,001. A exclusão da questão 5 elevou os índices de ajuste a níveis satisfatórios. A versão brasileira do HLS-14 foi considerada válida para mensurar literacia em saúde.

Abstract We evaluated the psychometric properties of the Health Literacy Scale - 14 (HLS-14), the Brazilian Portuguese version. In the methodological study with a cross-sectional design, the following were performed: translation, cross-cultural adaptation and evaluation of the psychometric properties. After being subjected to a committee of experts, translated and adapted, the instrument was pre-tested with 52 adults and applied to 143 adults and older people of Piracicaba-SP. Internal consistency was assessed based on the Kendall correlation coefficient and Cronbach’s alpha (>0.70), and the confirmatory factor analysis (CFA) was conducted using the chi-square test, the Standardized Residual Mean Square Root (SRMR), the Root Mean Square Error of Approximation (RMSEA) (<0.05), the Comparative Fit Index (CFI) (>0.95) and the Tucker-Lewis Index (TLI) (>0.95). The analysis of operational equivalence showed agreement between most of the questions, exception questions 6, 8, 9 and 14. Cronbach’s alpha was 0.82. There was reasonable adjustment in the CFA: CFI=0.886, TLI=0.86, RMSEA=0.085 (90%CI: 0.065-0.105), SRMR=0.071, chi-square (74 degrees of freedom) = 149.510, p<0.001. The exclusion of question 5 increased the adjustment level to satisfactory. The Brazilian Portuguese version of HLS-14 can be considered a valid health literacy assessment instrument.

https://doi.org/10.1590/1413-81232020257.22282018 1073 downloads

Tradução, adaptação transcultural e avaliação psicométrica da versão em português (brasileiro) do 14-item Health Literacy Scale
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Batista, Marília Jesus

; Marques, Ana Carolina de Paula

; Silva Junior, Manoelito Ferreira

; Alencar, Gizelton Pereira

; Sousa, Maria da Luz Rosário de

Ciência & Saúde Coletiva

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jul 2020, Volume 25 N. 7 Pages 2847 - 2857

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1413-81232020257.22282018

10.

In situ effect of a proanthocyanidin mouthrinse on dentin subjected to erosion
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CARDOSO, Fabrícia

; BOTEON, Ana Paula

; SILVA, Tamires Alves Pereira da

; PRAKKI, Anuradha WANG, Linda HONÓRIO, Heitor Marques

Journal of Applied Oral Science

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2020, Volume 28 elocation e20200051

Abstract: En | Text: En | PDF: En

Abstract Proanthocyanidin has been shown to be efficient in inhibiting matrix metalloproteinases. Objective The aim of this in situ study was to evaluate the protective effect of Proanthocyanidin-based mouthrinses either with naturally acidic or with a neutral pH applied on dentin subjected to erosion. Methodology Eight volunteers wore one palatal device in two phases (7 days washout) with 16 samples per group (n=8). The groups under study were: First Phase/ G1 – 10% proanthocyanidin mouthrinse (pH 7.0, Experimental group 1 – Purified Grape Seeds Oligomeric Proanthocyanidins), G2 – 10% proanthocyanidin mouthrinse (pH 3.0, Experimental group 2 – Purified Grape Seeds Oligomeric Proanthocyanidins). Second Phase/ G3 – 0.12% chlorhexidine mouthrinse (pH 7.0, Positive control group), G4 – no previous treatment (Negative control group). Each device was subjected to 3 erosive cycles (5 minutes) per day for 5 days. Treatments with different mouthrinses were applied once after the second erosive challenge (5 minutes). Profilometry was used to quantify dentin loss (µm). Results Data were analyzed by repeated measures of ANOVA followed by Fisher’s test (p<0.05). G1 (1.17±0.69) and G3 (1.22±0.25) showed significantly lower wear values with no statistical difference between them. G2 (2.99±1.15) and G4 (2.29±1.13) presented higher wear values with no significant differences between them. Conclusion The 10% proanthocyanidin mouthrinse (pH 7.0) could be a good strategy to reduce dentin wear progression.

https://doi.org/10.1590/1678-7757-2020-0051 579 downloads

11.

Problematization educational intervention to promote healthy habits in elderly people with diabetes: randomized clinical trial
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Borba, Anna Karla de Oliveira Tito

; Arruda, Ilma Kruze Grande de

; Marques, Ana Paula de Oliveira

; Leal, Márcia Carrera Campos

; Diniz, Alcides da Silva

; Linhares, Francisca Márcia Pereira

Revista Brasileira de Enfermagem

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2020, Volume 73 elocation e20190719

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: avaliar os efeitos de uma intervenção educativa problematizadora para a promoção de hábitos saudáveis em idosos com diabetes. Métodos: ensaio clínico randomizado, realizado com 202 indivíduos sorteados para dois grupos: intervenção e controle. A intervenção consistiu em atividades educativas grupais problematizadoras, mensalmente, ao longo de seis meses. O grupo controle participou do acompanhamento convencional na unidade de saúde. Os dados foram coletados mediante entrevistas semiestruturadas antes e após a intervenção, além da realização de exames laboratoriais. Resultados: após a intervenção, foi evidenciado aumento do conhecimento sobre a doença (p<0,001), atitude positiva para o autocuidado (p=0,011), prática de atividade física (p=0,020), variedade da dieta (p=0,002) e menor consumo de óleos e gorduras (p<0,05), quando comparado ao grupo controle. Conclusão: a intervenção realizada tem efeito benéfico para a promoção de hábitos saudáveis.

ABSTRACT Objective: to assess the effects of a problematization educational intervention to promote healthy habits in elderly people with diabetes. Methods: a randomized clinical trial conducted with 202 individuals drawn for the intervention group and the control group. The intervention consisted of problematization educational activities on a monthly basis for over six months. The control group participated in conventional monitoring at the health unit. Data were collected through semi-structured interviews before and after the intervention, in addition to laboratory tests. Results: after the intervention, when compared to the control group, there was an increase in knowledge about the disease (p<0.001), positive attitude towards self-care (p=0.011), physical activity (p=0.020), diet variety (p=0.002), and lower consumption of oils and fats (p<0.05). Conclusion: the intervention performed has a beneficial effect to promote healthy habits.

RESUMEN Objetivo: evaluar los efectos de una intervención educativa problemática para la promoción de hábitos saludables en personas mayores con diabetes. Métodos: ensayo clínico aleatorizado, realizado con 202 individuos elegidos para dos grupos: intervención y control. La intervención consistió en problematizar las actividades educativas grupales, mensualmente, durante seis meses. El grupo de control participó en el monitoreo convencional en la unidad de salud. Los datos fueron recolectados a través de entrevistas semiestructuradas antes y después de la intervención, además de las pruebas de laboratorio. Resultados: después de la intervención, un aumento en el conocimiento sobre la enfermedad (p<0.001), una actitud positiva hacia el autocuidado (p=0.011), actividad física (p=0.020), variedad de dieta (p=0.002) y menor consumo de aceites y grasas (p<0.05), en comparación con el grupo de control. Conclusión: la intervención realizada tiene un efecto beneficioso para la promoción de hábitos saludables.

https://doi.org/10.1590/0034-7167-2019-0719 934 downloads

12.

Problematization educational intervention to promote healthy habits in elderly people with diabetes: randomized clinical trial
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Borba, Anna Karla de Oliveira Tito

; Arruda, Ilma Kruze Grande de

; Marques, Ana Paula de Oliveira

; Leal, Márcia Carrera Campos

; Diniz, Alcides da Silva

; Linhares, Francisca Márcia Pereira

Revista Brasileira de Enfermagem

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2020, Volume 73 Suppl. 3 elocation e20190719

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

https://doi.org/10.1590/0034-7167-2019-0719 22 downloads

13.

Effect of first aid training on teams from special education schools
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Brito, Jackeline Gonçalves

; Oliveira, Inês Pereira de

; Godoy, Christine Baccarat de

; França, Ana Paula dos Santos Jesus Marques

Revista Brasileira de Enfermagem

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2020, Volume 73 N. 2 elocation e20180288

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivos: Analisar o efeito de uma capacitação no conhecimento da equipe multidisciplinar de escolas de ensino especializado sobre primeiros socorros diante de acidentes escolares. Métodos: Estudo quase experimental do tipo antes e depois com grupo único de comparação. Realizou-se estatística descritiva e teste de McNemar para avaliar o efeito da intervenção. Resultados: Participaram 162 profissionais de nível superior, predominantemente professores (82,1%). Sexo e faixa etária preponderante foram, respectivamente, feminino (97,5%) e idade acima de 40 anos (69,2%). Houve aumento de acerto em todas as questões abordadas com significância estatística (≤ 0,05). Destacam-se o correto manejo diante de queda com traumatismo craniano encefálico, choque elétrico e queimadura por líquido quente (respectivamente: 98,1%, 98,1% e 96,9% de acertos). Conclusões: A capacitação sobre primeiros socorros diante de acidentes, por meio de exposição de conteúdo de forma dialogada e prática, se mostrou eficiente para a equipe multidisciplinar de escolas de ensino especializado para pessoas com deficiência.

ABSTRACT Objectives: Analyze the effect of first aid training on the knowledge of multidisciplinary teams from special education schools, in school accidents. Methods: A quasi-experimental, before-and-after study with a single comparison group. Descriptive statistics and McNemar’s test were used to evaluate the effect of the intervention. Results: This study had the participation of 162 higher education professionals, predominantly teachers (82.1%), female (97.5%), aged over 40 (69.2%). An increase in correct answers was observed, with statistical significance (≤0.05), especially in proper handling in case of fall with traumatic brain injury, electric shock, and burn due to hot liquid (98.1%, 98.1% and 96.9% of proper response, respectively). Conclusions: First aid training for child accidents, through content exhibition, in a dialogical and practical way, proved to be efficient for multidisciplinary teams from special education schools for people with disabilities.

RESUMEN Objetivos: Evaluar el efecto de la capacitación sobre los primeros auxilios ante accidentes escolares en el conocimiento del equipo multidisciplinario de escuelas de enseñanza especializada. Métodos: Estudio cuasiexperimental con análisis antes y después de un único grupo de comparación. Se realizaron la estadística descriptiva y la prueba de McNemar para evaluar el efecto de la intervención. Resultados: Participaron 162 profesionales de nivel superior, principalmente profesores (82,1%). El sexo y el grupo de edad predominantes fueron el femenino (97,5%) y la edad superior a 40 años (69,2%). Se observó un incremento de aciertos con significancia estadística en todas las cuestiones abordadas (≤ 0,05). Se destacan el manejo correcto ante la caída con traumatismo craneal encefálico, el choque eléctrico y la quemadura por líquido caliente (con un 98,1%, un 98,1% y un 96,9% de aciertos, respectivamente). Conclusiones: La capacitación sobre primeros auxilios ante accidentes, por medio de exposición de contenido de forma dialogada y práctica, fue eficaz para el equipo multidisciplinario de escuelas de enseñanza especializada para personas con discapacidad.

https://doi.org/10.1590/0034-7167-2018-0288 5619 downloads

14.

Effect of first aid training on teams from special education schools
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Brito, Jackeline Gonçalves

; Oliveira, Inês Pereira de

; Godoy, Christine Baccarat de

; França, Ana Paula dos Santos Jesus Marques

Revista Brasileira de Enfermagem

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2020, Volume 73 N. 2 elocation e20180288

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

https://doi.org/10.1590/0034-7167-2018-0288 109 downloads

15.

NA FRONTEIRA DO MERCADO DE EMPREGO: JOVENS, TRABALHO E CIDADANIA
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Marques, Ana Paula Pereira

Trabalho, Educação e Saúde

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2020, Volume 18 Suppl. 1 elocation e0024981

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo Os riscos de precarização e maior vulnerabilidade dos jovens às recentes políticas econômicas e financeiras restritivas em Portugal configuram transformações significativas nos seus percursos educativos e profissionais. As transições para o mercado de trabalho tornam-se incertas e subjetivas, comprometem a capacidade dos jovens de projetarem os seus futuros e oportunidades profissionais. Este artigo destaca os processos de diferenciação que instauram desigualdades sociais nos itinerários de transição profissional. Ao combinar uma metodologia que articula estatísticas oficiais e evidências empíricas de estudos, sustenta-se uma análise crítica das fragilidades do sistema educativo e do mercado de trabalho português no contexto da União Europeia. Dos principais resultados, destacam-se as fragilidades de inserção dos jovens, visíveis na maior incidência ao desemprego e a empregos precários e maior restrição no acesso à proteção de direitos sociais. Os (novos) riscos ampliam-se, acentuando a individualização nos processos de recrutamento e a inexistência de carreiras e planejamento do futuro profissional, o que contribui para um agravamento das desigualdades (inter)geracionais e sociais na relação com o trabalho e o emprego.

Abstract The risks of precariousness and greater vulnerability of young people to the restrictive economic and financial policies of the last years in Portugal are significant changes in their educational and professional pathways. Transitions to the job market become uncertain and subjective, compromising young people’s ability to project their future and career opportunities. This article highlights the differentiation processes that create visible social inequalities in the professional transition itineraries. Combining a methodology that articulates official statistics and empirical evidence from studies, we carried out a critical analysis of the weaknesses of the Portuguese education system and labor market in the context of the European Union. Among the main results, we highlight the weaknesses of youth insertion, visible in the higher incidence of unemployment, precarious jobs and greater restriction in access to the protection of social rights. The (new) risks increase, emphasizing the individualization in the processes of recruitment and lack of careers and planning of the professional future, which contributes to an aggravation of (inter) generational and social inequalities in relation to work and employment.

Resumen Los riesgos de precariedad y de mayor vulnerabilidad de los jóvenes a las políticas económicas y financieras restrictivas de los últimos años configuran transformaciones significativas en sus itinerarios educativos y profesionales. Las transiciones para el mercado laboral se convierten inciertas y subjetivas, comprometiendo la capacidad de los jóvenes para planificar sus futuros y oportunidades profesionales. Este artículo destaca los procesos de diferenciación que establecen desigualdades sociales visibles en los itinerarios de transición profesional. Al combinar una metodología que articula estadísticas oficiales y evidencias empíricas de estudios conducidos por nosotros, se soporta un análisis crítico de las debilidades del sistema educativo y del mercado laboral portugués en el contexto de la Unión Europea. Entre los principales resultados se destacan las debilidades de inserción de los jóvenes, visibles en la mayor incidencia de desempleo, empleos precarios y mayor restricción en el acceso a la protección de derechos sociales. Los (nuevos) riesgos aumentan, acentuando la individualización en los procesos de reclutamiento e inexistencia de carreras o planeamiento del futuro profesional, que contribuye al agravamiento de las desigualdades (inter)generacionales y sociales en la relación con el trabajo y empleo.

https://doi.org/10.1590/1981-7746-sol00249 2010 downloads

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