Aim This study aimed to assess the prevalence of temporomandibular disorders (TMD) and psychosocial comorbidities in undergraduate dental students in a southern Brazilian university, during the COVID-19 pandemic. Also, it aimed to verify the association between psychosocial factors and TMD. Methods Fonseca Anamnestic Index, Depression Anxiety Stress Scale (DASS-21), a socioeconomic questionnaire, and questions about academic performance and social distancing were applied. Poisson regression analysis was used to assess the association of predictive variables with TMD. Results The prevalence of TMD was found to be 82.4%, and more than half of the students had some degree of stress, anxiety, and depression. Students who had symptoms of stress (RR 1.11; 95% CI 1.04-1.19), anxiety (RR 1.19; 95% CI 1.12-1.27) and reported academic performance worsening (RR 1.12; 95% CI 1.07-1.19) had higher TMD scores. Conclusion The findings suggest that TMD was highly prevalent among dental students at a federal university in southern Brazil during the pandemic, being associated with high levels of stress, anxiety, poor academic performance, and greater social distancing.

Abstract The aim of this study was to understand the influence of orthosurgical treatment on oral health-related quality of life (OHRQoL) in patients with Class II and III skeletal malocclusion by conducting a mixed method case series study. Nineteen patients submitted to orthosurgical treatment in a private practice in Brazil were included in the sample. Data were collected retrospectively and interviews were held from March 2020 to July 2021. Patients answered to the Oral Health Impact Profile (OHIP-14) in the first part of the interview and subsequently answered the qualitative questions. The overall mean of OHIP-14 after treatment was 4.21 (SD 4.68). The qualitative data were analyzed according to thematic analysis and four themes emerged from the interviews: a) concept of quality of life, b) pre-treatment life, c) post-treatment life, and d) positive and negative aspects of treatment. Quality of Life was reported by the patients as the absence of feeling pain, having emotional and physical health, having a satisfactory esthetic appearance and self-esteem. Before treatment, most Class II patients used to complain about breathing and sleeping problems, while Class III patients complained more intensely about esthetics. Pain was a common problem reported by both Class II and Class III patients. In general, improvement was perceived in self-esteem, esthetics, function and pain. Complaints about negative aspects of the treatment were restricted to the postoperative period. The orthosurgical treatment was important for improving the OHRQoL of patients in terms of esthetic, functional and psychosocial aspects.

Abstract Background Malocclusions are highly prevalent in childhood and adolescence, being considered a public health problem worldwide, in addition to be considered an important predictor in the tongue position and speech disorders. Objective Evaluate the association of malocclusions with tongue position and speech distortion in mixed-dentition schoolchildren from the south of Brazil. Methodology This cross-sectional study was performed using a database of an epidemiological survey realized in the southern of Brazil, in 2015, for evaluating the dental and myofunctional condition of the mixed-dentition from 7-13 years’ schoolchildren. The outcome variables were tongue position and speech distortion, evaluated by a trained and calibrated examiner. Characteristics regarding sociodemographic and oral health measures (Angle’s classification of the malocclusion, overjet, overbite, posterior crossbite and respiratory mode) were also assessed. Poisson regression models with adjusted robust variance were used to evaluate the association among predictors variables in the outcomes. Results are presented as prevalence ratio (PR) and 95% confidence interval (95% CI). Results A total of 547 children were evaluated. Schoolchildren who presented anterior open bite (PR 2.36 95%CI 1.59-3.49) and having oral/oral-nasal breathing (RP 2.51 95%CI 1.70-3.71) are more likely to have altered position of the tongue. Both deep bite and being male represent protection factors for the abnormal tongue position. Regarding speech distortion, deep overbite presents a protective relationship to speech distortion (PR 0.41; 95%CI 0.24-0.71), whereas schoolchildren with posterior crossbite were more likely to present this problem (PR 1.77; 95%CI 1.09-2.88). Conclusion Anterior open bite and posterior crossbite were the malocclusions related to speech distortion and/or altered tongue position. Oral/oral-nasal breathing was also related to myofunctional changes. Deep bite malocclusion was a protective factor for both speech problems and altered tongue position when compared to a normal overbite.

ABSTRACT. The accumulation of industrial by-products increases the use of sweet potato waste for ruminants, but ruminal pH characteristics are still not well known. The objective was to assess the fluctuation of ruminal pH in sheep supplemented with different levels of sweet potato flour inclusion in their diet. Four rumen-fistulated sheep were used; they were fed a diet based on ryegrass haylage (Lolium multiflorum) and sweet potato flour (Ipomoea batatas), provided according to the level of inclusion in the total diet (0, 0.5, 1.0 and 1.5%). Approximately 80 ml of ruminal fluid was collected for reading on a bench pH meter. Statistical data analysis was run on Statistical Analysis System (SAS Institute INC. Cary, NC, USA), and statistical difference was considered for p < 0.05. The animals that received 1.5% of sweet potato flour in their diet presented acid rumen pH; the 1.0% group presented rumen pH acidification in the first 6 hours after feeding, and the 0.5% level of inclusion did not change the rumen environment. It is concluded that the inclusion of 0.5% sweet potato flour in sheep diet proved to be an efficient energy supplementation strategy.

RESUMO: A biomassa microbiana é determinante na dinâmica da matéria orgânica do solo e sua atividade é um indicador de qualidade do solo em agroecossistemas, refletindo mudanças em práticas de manejo e em condições ambientais. Avaliou-se o efeito de culturas de cobertura de inverno, solteiras ou consorciadas, sobre atributos químicos do solo, carbono da biomassa microbiana (CBM), respiração basal (RB), quociente metabólico (qCO2) e atividade das enzimas urease, β-glucosidase e FDA, bem como o rendimento da cebola em um sistema de plantio direto. O solo é um Cambissolo Húmico alumínico, os tratamentos foram testemunha com vegetação espontânea, cevada, centeio, nabo forrageiro (NF), NF + centeio e NF + cevada. Realizaram-se cinco coletas de amostras de solo (0-10 cm) entre junho a dezembro. Não houve diferenças entre os tratamentos para CBM e RB, e os maiores valores para esses atributos ocorreram em junho, quando as plantas de cobertura estavam em seu estágio inicial. Embora o qCO2 não tenha sido afetado por nenhum tratamento, ele variou entre os períodos de amostragem, com valores entre 0,62 e 10 µg de C-CO2 mg-1 MBC h-1, indicando um ambiente de baixo ou nenhum estresse. As culturas de cobertura tiveram pouca influência na atividade enzimática, mas o FDA foi reduzido em áreas com cultivos solteiros de cevada ou centeio. O rendimento médio de cebola nos tratamentos com culturas de cobertura foi de 13,01 (Mg ha-1), mais alto que no tratamento controle.

ABSTRACT: Microbial biomass is a driving force in the dynamics of soil organic matter, and microbial activity is an indicator of soil quality in agroecosystems, reflecting changes in management practices and environmental conditions. We evaluated the effect of monoculture and intercropped winter cover crops on soil chemical attributes, microbial biomass carbon (MBC), basal respiration (BR), metabolic quotient (qCO2), urease, β-glucosidase, and fluorescein diacetate (FDA) hydrolysis activity, as well as onion yield in a no-tillage system. Soil is a Typic Humudept, and treatments were control with spontaneous vegetation, barley, rye, oilseed radish (OR), OR + rye, and OR + barley. The soil was sampled (0-10 cm) five times between June and December. There were no differences among treatments for MBC and BR, and the highest values for those attributes occurred in June, when cover plants were in their initial stage. Although, qCO2 was not affected by any treatment, it varied among sampling periods, ranging from 0.62 to 10 µg C-CO2 mg-1 MBC h-1, indicating a low- or no stress environment. Cover crops had little influence on enzyme activity, but FDA was lowered in areas with single crops of barley and rye. Average onion yield in cover crops treatments was 13.01 (Mg ha-1), 30-40% higher than in the control treatment.

RESUMO Este artigo apresenta os resultados da análise estatística dos parâmetros microbiológicos, físicos e químicos relacionados à qualidade da água utilizada em campos de arroz no sul do Brasil. Os dados foram coletados durante três anos agrícolas consecutivos, dentro da estrutura de um programa abrangente de monitoramento. Os indicadores utilizados foram: potencial de hidrogênio, condutividade elétrica, turbidez, nitrogênio, fósforo, potássio, cálcio, coliformes totais e fecais. Componente Principal e Análise Discriminante indicaram diferenças consistentes entre a água de irrigação e de drenagem, da mesma forma a variação temporal demonstrou uma clara redução na concentração da maioria das variáveis analisadas. O padrão desta redução não é o mesmo nas duas regiões - isto é, a importância de cada uma das diferentes variáveis nas duas localizações é alterada. Estes resultados sugerem que as variações na qualidade da água utilizada para a irrigação do arroz foi influenciada por alguns aspectos específicos de cada região do arroz no sul do Brasil - como ação antrópica ou as condições de solo/clima em cada bacia hidrográfica.

ABSTRACT This paper presents the results of the statistical analysis of microbiological, physical and chemical parameters related to the quality of the water used in rice fields in Southern Brazil. Data were collected during three consecutive crop years, within structure of a comprehensive monitoring program. The indicators used were: potential hydrogen, electrical conductivity, turbidity, nitrogen, phosphorus, potassium, calcium, total and fecal coliforms. Principal Component and Discriminant Analysis showed consistent differences between the water irrigation and drainage, as the temporal variation demonstrated a clear reduction in the concentration of most of the variables analyzed. The pattern of this reduction is not the same in the two regions - that is, the importance of each of the different variables in the observed differentiation is modified in two locations. These results suggested that the variations in the water quality utilized for rice irrigation was influenced by certain specific aspects of each rice region in South Brazilian - such as anthropic action or soil/climate conditions in each hydrographic basin.

INTRODUÇÃO: A detecção de estenose de artéria renal em pacientes hipertensos pode ser um sinal de aterosclerose arterial sistêmica. OBJETIVOS: Identificar e caracterizar do ponto de vista clínico e epidemiológico os pacientes hipertensos com estenose de artéria renal, avaliando fatores de risco cardiovascular e presença de doença aterosclerótica multiarterial sintomática. MÉTODO: Foram selecionados os pacientes hipertensos atendidos no ambulatório de Nefrologia da Universidade Federal do Triângulo Mineiro (UFTM) entre 2000-2010, com diagnóstico de estenose de artéria renal de etiologia aterosclerótica. Avaliaram-se dados epidemiológicos (gênero, idade, etnia), fatores de risco cardiovascular (Diabetes Mellitus, hipercolesterolemia, hipertrigliceridemia, tabagismo, síndrome metabólica), informações relativas à hipertensão (tempo de diagnóstico, histórico familiar, número de medicamentos utilizados), eventos cardiovasculares prévios (infarto agudo do miocárdio, acidente vascular encefálico isquêmico, doença arterial periférica). Estratificaram-se os níveis pressóricos, risco cardiovascular global e escore Framingham. RESULTADOS: Casuística de 30 pacientes, maioria feminina (73,3%), média de idade de 66 anos, 86,67% brancos. Tempo médio de HAS de 19,94 anos, 89,28% sem histórico familiar, 13,8% com diabetes, 65,51% tabagistas, 17,25% com hipertrigliceridemia, 62,06% com hipercolesterolemia e 66,7% com síndrome metabólica. Número médio de medicamentos em uso: 3,26. Estenose de artéria renal predominante à direita quando isoladamente (46,7%) e em terço proximal (56,7%). Creatinina elevada em 40% dos pacientes. Quanto ao estágio de hipertensão, maioria estágio 2 (47%) e 73,3% com risco cardiovascular global alto. Escore Framingham Médio de 13%. 66,7% apresentavam doença aterosclerótica em outro sítio, sendo coronariano o principal (53,3%). CONCLUSÃO: A correlação mais comum foi com o infarto agudo do miocárdio, o que implica na busca do comprometimento coronário quando do diagnóstico de estenose de artéria renal em pacientes hipertensos, para tentar evitar danos futuros ao paciente.

INTRODUCTION: The detection of the renal artery stenosis in hypertensive patients can be a signal of systemic arterial atherosclerosis. AIMS: To identify and characterize clinical-epidemiologically the hypertensive patients with renal artery stenosis, evaluating factors of cardiovascular risk and presence of symptomatic multiarterial atherosclerotic. METHOD: Were selected the hypertensive patients who were assisted at the Nephrological Clinic of Universidade Federal do Triângulo Mineiro (UFTM) between 2000-2010, with diagnosis of renal artery stenosis of atherosclerotic etiology. Epidemiological data were evaluated (gender, age, ethnicity), factors of cardiovascular risk (diabetes, hypercholesterolemia, hypertriglyceridemia, tabagism, metabolic syndrome), information on hypertension (time of diagnosis, family report, number of used medicines), previous cardiovascular events (acute myocardial infarctation, ischemic stroke, peripheral arterial disease). Blood pressure levels, global cardiovascular risk and Score Framingham were stratified. RESULTS: Casuistry of 30 patients, feminine majority (73.3%), average of 66 year-old age, 86.67% white, medium time of hypertension of 19.94 years, 89.92 without family report, 13.8 with diabetes, 65.51% smoking, 17.25% hypertriglyredemia, 62.06% with hypercholesterolemia and 66.7% with metabolic syndrome. Average number of medicines in use: 3.26. Dominant right-sided renal artery stenosis separately (46.7%) and in proximal third (56.7%). High creatinine levels in 40% of the patients. As for the hypertension phase, majority phase 2 (47%) and 73.3% with high global cardiovascular risk. Average Framingham Score of 13%. 66.7% presented atherosclerotic disease in another place, being infarctation the main one (53.3%). CONCLUSION: The most common correlation was with acute myocardial infarctation, what implicates in the search of the coronary compromising to the diagnosis of renal artery stenosis in hypertensive patients to try avoid future damages to the patient.

O objetivo deste trabalho é apresentar os principais resultados da pesquisa sobre o leite instável não ácido ou LINA. A proporção de amostras de leite com estabilidade térmica, abaixo do mínimo exigido pela indústria brasileira, configura um sério problema, com uma ocorrência mais elevada durante os períodos de carência alimentar. Trata-se de um problema multifatorial, cujos fatores intervenientes são relacionados à execução do teste (concentração do álcool), ao manejo (alimentação, clima, relação homem-animal), ao animal (suscetibilidade ao estresse, potencial produtivo, estádio da lactação, sanidade, problemas digestivos e metabólicos, frações da caseína), entre outros. Existem dúvidas quanto à capacidade do teste do álcool em estimar a estabilidade térmica do leite. A indústria necessita de um teste rápido, de baixo custo e que realmente identifique o leite adequado ao processamento térmico. O manejo correto dos animais, aliado ao uso de parâmetros realistas de execução do teste do álcool, pode contribuir para melhorar a estabilidade do leite.

This paper aimed to present the main results about the unstable non acid milk (LINA). The proportion of milk samples with thermal stability below the Brazilian dairy industry standard is a serious problem, with highest occurrence at short food supply periods. It is a multi factorial problem, which causing factors are related to test execution (ethanol concentration used), to management (feeding, climate, human-animal relation), to animal (stress susceptibility, productive potential, lactation stage, sanitary condition, digestive and metabolic disorders), among others. There are doubts to the accuracy and precision of the alcohol test to detect stability problems. Dairy industry needs a fast, low price and easy test, but that can identify milk suitable to thermal processing. An adequate animal's management besides realistic parameters of milk test might contribute to improve milk stability.

Bacilos entomopatogênicos são bactérias observadas principalmente no solo, consideradas importantes agentes no controle biológico de doenças e pragas da agricultura. No entanto, existem poucos estudos que relatam a influência das práticas agrícolas nas populações de Bacillus spp. Essa pesquisa objetivou avaliar a freqüência de bactérias entomopatogênicas pertencentes ao gênero Bacillus obtidas de amostras de solo coletadas no ano agrícola de 2006/2007 de diferentes sistemas de cultivo de arroz irrigado da EEA-IRGA, Cachoeirinha (RS). Foram analisadas 36 amostras de solos, oriundas de três sistemas de plantio de arroz irrigado, pré-germinado (SPG), convencional (SPC), plantio direto (SPD) e de uma área sem plantio de arroz (C). Para tanto, um grama de solo foi diluído, pasteurizado e inoculado em Ágar Nutriente (AN), incubado a 30 °C, por 24 horas. Todas as unidades formadoras de colônias bacterianas (UFC) foram repicadas em meios seletivos e as espécies, B. thuringiensis, B. sphaericus e B. cereus, foram identificadas por microscopia de contraste de fase. Os resultados revelaram que não houve diferença significativa entre os sistemas de plantio analisados. Por outro lado, a frequência de Bacillus spp. variou entre as diferentes fases do plantio de arroz nos períodos amostrados. A irrigação foi o principal fator que contribuiu para a variação da frequência dos Bacillus em sistemas de plantio de arroz irrigado da EEA-IRGA.

Entomopathogenic Bacillus are bacteria found mainly in the soil and are considered important agents in biological control of agricultural diseases and pests. However, there are fen studies describing the influence that different agricultural practices have on Bacillus spp. populations. This study proposes to determine the occurrence frequency of entomopathogenic bacteria from Bacillus genus in soil samples. These were collected during the agricultural year 2006/07 from different irrigated rice cultivation systems used in the EEA-IRGA plantations in Cachoerinha, State of Rio Grande do Sul, Brazil. We analyzed 36 soil samples taken from three irrigated rice plantation systems; (i) Pre-germinated (SPG); (ii) Conventional (SPC); and (iii) Direct plantation (SPD) and a fourth sample from a non-planted area (C). In each test one gram of soil was diluted, pasteurized and inoculated in Nutrient Agar (NA) and incubated for 24 hours at 30°C. All the bacteria colony-forming units (CFU) were transplanted to selective mediums and the B. thuringiensis, B. sphaericus and B. cereus species were identified using a phase contrast microscope. No significant differences in the occurrence frequencies were found in the soil samples of all plantation systems analyzed. On the other hand, the Bacillus spp frequency varied in the sampled period during the different growing phases of the irrigated rice in the EEA-IRGA plantations evaluated. Irrigation was the main factor contributing to variation of the Bacillus in the plantations.

No presente estudo investigamos o polimorfismo em três espécies de Chauliognathus Hentz, 1930 (Coleoptera, Cantharidae), analisando a variação geográfica no padrão de coloração dos élitros em C. flavipes, C. fallax e C. octomaculatus. Estas espécies pertencem ao complexo mülleriano chamado "amarelo-preto". São encontradas formando grandes agregados sobre as plantas que utilizam como substratos de alimentação e apresentam semelhança morfológica e para a coloração dos élitros e pronoto. Chauliognathus flavipes é a espécie mais freqüente e apresenta o maior número de padrões fenotípicos (8) seguidos de C. fallax e C. octomaculatus com seis e dois padrões respectivamente. Diferenças significativas nas freqüências dos padrões de coloração nas três espécies foram detectadas ao longo da área analisada. Em geral, as diferenças foram observadas entre as regiões do Estado do Rio Grande do Sul (p < 0,05), mas não dentro das regiões, exceto para a região Metropolitana. Nas três microregiões que formam a região Metropolitana encontrou-se uma correlação positiva entre o aumento da altitude e a diversidade em C. flavipes e C. fallax (r = 0,860, p < 0,01; r = 0,974, p < 0,01, respectivamente).

In the present report, we investigate polymorphism in three of the Brazilian species of Chauliognathus Hentz, 1930 (Coleoptera, Cantharidae), by analyzing the geographical color pattern variation of the elytra in C. flavipes, C. fallax and C. octomaculatus. These species belong to the mullerian complex called the "yellow-black". They are usually found in clusters on their host plants at various locations and present similarities in the external body morphology and in the color pattern of the elytra and the pronotum. Chauliognathus flavipes is the most common species with eight phenotypic classes followed by of C. fallax and C. octomaculatus with six and two phenotypic classes respectively. The analyses indicate a statistically significant difference in the frequencies of colour patterns of the three species over the entire area sampled. In general, the significant differences were observed between the regions of the State of Rio Grande do Sul (p < 0.05) but were not found within the individual regions except in the case of the Metropolitan region. That region and its three microregions demonstrated a positive co-relationship between an increase in altitude and the occurrence of diversity in C. flavipes and C. fallax (r = 0.860, p < 0.01; r = 0.974, p < 0.01, respectively).

The phylogenetic relationships of twelve species of Chauliognathus were investigated by studying the mitochondrial cytochrome oxidase I gene. A 678 bp fragment of the COI gene was sequenced to test the hypothesis that the Müllerian mimicry species of the "yellow-black" complex make up a monophyletic clade, separated from species with other colour patterns. The data set was analysed by neighbour-joining, maximum parsimony and maximum likelihood procedures. The results support a single origin of the yellow-black colour pattern during the evolution of the genus, with one main clade formed by Chauliognathus lineatus, C. tetrapunctatus, C. riograndensis, C. flavipes, C. octomaculatus, C. fallax, and another one formed by two species, C. expansus and C sp 1, plus an orange-black-coloured species. The nucleotide divergences found between C. sp 3 (black) and the other species studied fall within the level expected for species from different genera. The similarity of colour patterns of the yellow-black species has been considered an example of Müllerian mimicry by conservation of the ancestral state with some minor modifications.

Beetles of the species Chauliognathus fallax Germar 1824 are polymorphic for elytron colouration with six morphs distinguished on the basis of black pigmentation on a yellow background. We investigated samples of C. fallax taken in eight consecutive weeks aiming to determine the frequency of the morphs which were grouped, for statistical analysis, into three classes according to elytra pigmentation as well as the possible occurrence of assortative mating. Our results showed a variation in the frequency of the classes throughout the season, both in males and females, with the maximum frequency of each class at the fourth and fifth week. The three classes (A, B, C) showed the same pattern of variation, and class B was always the more frequent. To test randomness of matings two approaches were taken: in the first, we compared the frequency of each class in copulating and non-copulating insects. In the second, the frequency of each class in the whole sample was taken as the probability of occurrence of the respective class; then, using the criterion of the probability of independent events we calculated the expected proportion of copulating insects for each pair of events. Both methods gave non-significant differences, suggesting that the matings were random.

The relative population sizes of a species complex of Chauliognathus are reported, as well as their spatial distribution associated with different patches of food plants. Field work was done at Fazenda Santa Isabel, municipality of Guaíba, State of Rio Grande do Sul, Brazil. The results suggest that two mechanisms account for the reduction in food competition among the species involved: one is asynchrony in the appearance of the species in the area, and the other is aggregation in different patches of food plants. Since the species here reported show a similar colour pattern (yellow-black) the possibility of the occurrence of serial mimicry in this complex of species is dicussed.

This study presents data on morphometric variation for length and width of elytra in tive sympatric species of the genus Chauliognathus Hentz, 1830: C. flavipes Fabricius, 1781, C. octomaculatus Pie, 1915, C. expansus Waterhouse, 1878, C. fallax Germar, 1824 and C. lineatus Zwetsch & Machado, 2000. The meaning of this variation is discussed in the light of the theory of mimicry and visual communication between prey and predator. Females are larger than males in all species, except C. expansus. The analysis of variance for length of elytron as well as for width showed that the differences between species are significant for males and females; significant interaction (sex x species) was also found. The similarity in color pattern of these species, as well as similarities in the morphometric analysis, suggests that they could form a mimetic ring of the Müllerian type, which the authors suggest be called, a "yellow-black" complex.

This study presents data on the color polymorphism in Chauliognathus flavipes Fabricius 1781 (Coleoptera, Cantharidae), analysing the variations in the emergence pattern of phenotypic classes for elytra color and mating system. The frequency of the phenotypic classes varies significantly throughout the season (males:X² = 59,705; females: X² = 61,017; 10 gl; p<0.001) being similar in males and females. The frequency of classes does not differ significantly among the insects that were copulating and those that were not copulating at the time of collection, i.e., the matings are random for elytra color patterns. The variations observed in frequency of phenotypic classes are not influenced by factors associated with sex. Furthermore, the elytra color patterns probably do not influence the individuals' ability to copulate.