ABSTRACT Objective: To evaluate the relationship between the maternal diet quality and the fatty acid composition of breast milk in the first trimester of lactation. Methods: This is an observational cross-sectional epidemiological study of nursing mothers. The data collection instruments were a semi-structured questionnaire for sample characterization and a recall of usual intake. Diet quality was assessed based on the healthy eating index (HEI). Samples of mature breast milk were obtained by hand milking. Milk fat was extracted using the Bligh-Dyer method and methylated with 0.25 mol/L sodium methoxide in methanol diethyl ether. A gas chromatograph equipped with a flame ionization detector determined the milk fatty acid profile. Pearson’s and Spearman’s correlation tests evaluated association between the variables. Subsequently, a multiple linear regression model was built and multivariate regression analysis was applied. Results: Our findings revealed an inverse relationship between the consumption of total fruits and the polyunsaturated fatty acid profile and a direct association of the intake of total fruits and total grains with monounsaturated and saturated fatty acids. Conclusions: The results of this study suggest that maternal diet quality affects the fatty acid composition of breast milk.

RESUMO Objetivo: Avaliar a relação entre a qualidade da dieta materna e a composição do leite humano em ácidos graxos no primeiro trimestre de lactação. Métodos: Trata-se de um estudo epidemiológico observacional e transversal com nutrizes. Os instrumentos de coleta dos dados foram questionário semiestruturado para caracterização da amostra e o recordatório de ingestão habitual. A qualidade da dieta foi avaliada por meio do índice de alimentação saudável (IAS). Amostras de leite materno maduro foram obtidas por meio de ordenha manual. A extração da gordura do leite ocorreu mediante o método de Bligh-Dyer, e essa gordura foi metilada com metóxido de sódio 0,25 mol/L em metanol dietil éter. O perfil de ácidos graxos do leite foi determinado por um cromatógrafo a gás equipado com detector por ionização de chamas. Realizaram-se teste de correlação de Pearson e teste de Spearman para verificar associação entre as variáveis. Posteriormente se aplicaram o modelo de regressão linear múltiplo e a análise de regressão multivariada. Resultados: Foi possível observar relação inversa entre o consumo de frutas totais e o perfil de ácidos graxos poli-insaturados e relação direta entre o consumo de frutas totais e cereais totais e os ácidos monoinsaturados e saturados. Conclusões: Sugere-se que a qualidade da dieta reflete na composição de ácidos graxos do leite materno.

Abstract This article sets out to identify the socio-demographic and nutritional factors associated with the nutritional status of iodine in the maternal-infant group. The review was based on the Prisma method and the Medline (PubMed), Scopus and Lilacs databases were consulted. The search was conducted in December 2018, using the key words nutritional status, iodine, infant, pregnant and lactating. The selection followed the stages of excluding duplicated articles, reading the titles, abstracts and texts in full. A total of 1,500 studies were identified and 26 met the inclusion criteria. The methodological quality of the studies was evaluated using the questionnaire proposed by Downs and Black. There was an association between the sociodemographic factors of income, education, maternal and gestational age, uniformity and place of residence in rural or urban areas with the nutritional state of iodine. In relation to dietary factors, the consumption of supplements, iodized salt and dietary sources of iodine, especially dairy products, play a protective role to avoid the deficiency. It is necessary to create more specific and effective strategies that address all the factors that affect the nutritional state of iodine to avoid the disorders resulting from the deficiency.

Resumo O objetivo deste artigo é identificar os fatores sociodemográficos e alimentares associados ao estado nutricional de iodo do grupo materno-infantil. A revisão foi baseada no método PRISMA. As bases consultadas foram: Medline (PubMed), Scopus e Lilacs. A busca ocorreu em dezembro de 2018, utilizando os descritores estado nutricional, iodo, lactente, gestante e lactante. A seleção seguiu as etapas de exclusão dos duplicados, leitura dos títulos, resumos e análise na íntegra. Foram identificados 1.500 estudos e 26 contemplaram os critérios de inclusão. A qualidade metodológica dos estudos foi avaliada pelo questionário proposto por Downs e Black. Houve associação entre os fatores sociodemográficos renda, escolaridade, idade materna e gestacional, paridade e local de residência rural ou urbano com o estado nutricional de iodo. Já em relação aos fatores alimentares, o consumo de suplementos, sal iodado e alimentos fontes de iodo, em especial os produtos lácteos, exercem papel protetor contra a deficiência. É preciso criar estratégias mais específicas, efetivas e que contemplem todos esses fatores que afetam o estado nutricional de iodo para evitar as desordens provocadas pela deficiência.

ABSTRACT As pregnant women are susceptible to changes in iodine, which can cause miscarriage, goiter, thyroid nodules, hypothyroidism, in addition to fetal neurological impairment or development. The aim of this study was to verify the implications of the iodine alteration in each gestational trimester and its consequences of physiological justification. The review was based on PRISMA. Searching for articles that took place in March 2020 without delimiting data. As bases consulted were the Clinical Trials, Cochrane Library, Lilacs and Medline (PubMed). The descriptors were combined as follows: "pregnancy" AND "iodine deficiency". Articles that addressed iodine deficiency and its implications were included. The selection followed the steps of reading the titles, abstracts and full articles. To assess the methodological quality of the studies, the STROBE Instruction instrument was used. The research resulted in 1,266 studies and 11 were included. In assessing methodological quality, the lowest score was and the maximum 20. According to studies, the fourth most affected by iodine loss are the second and third, it is possible to increase the volume and pneumatic nodules, subclinical hypothyroidism, pre-eclampsia, among others. The damages caused by iodine deficiency in the first or second trimester are still reversible, therefore, they need to be diagnosed early, to guarantee an iodic homeostasis and prevent damage to the health of the mother-child binomial.

ABSTRACT Background: More than one-third of COVID-19 patients present neurological symptoms ranging from anosmia to stroke and encephalopathy. Furthermore, pre-existing neurological conditions may require special treatment and may be associated with worse outcomes. Notwithstanding, the role of neurologists in COVID-19 is probably underrecognized. Objective: The aim of this study was to report the reasons for requesting neurological consultations by internists and intensivists in a COVID-19-dedicated hospital. Methods: This retrospective study was carried out at Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil, a 900-bed COVID-19 dedicated center (including 300 intensive care unit beds). COVID-19 diagnosis was confirmed by SARS-CoV-2-RT-PCR in nasal swabs. All inpatient neurology consultations between March 23rd and May 23rd, 2020 were analyzed. Neurologists performed the neurological exam, assessed all available data to diagnose the neurological condition, and requested additional tests deemed necessary. Difficult diagnoses were established in consensus meetings. After diagnosis, neurologists were involved in the treatment. Results: Neurological consultations were requested for 89 out of 1,208 (7.4%) inpatient COVID admissions during that period. Main neurological diagnoses included: encephalopathy (44.4%), stroke (16.7%), previous neurological diseases (9.0%), seizures (9.0%), neuromuscular disorders (5.6%), other acute brain lesions (3.4%), and other mild nonspecific symptoms (11.2%). Conclusions: Most neurological consultations in a COVID-19-dedicated hospital were requested for severe conditions that could have an impact on the outcome. First-line doctors should be able to recognize neurological symptoms; neurologists are important members of the medical team in COVID-19 hospital care.

RESUMO Introdução: Mais de um terço dos pacientes com COVID-19 apresentam sintomas neurológicos que variam de anosmia a AVC e encefalopatia. Além disso, doenças neurológicas prévias podem exigir tratamento especial e estar associadas a piores desfechos. Não obstante, o papel dos neurologistas na COVID-19 é provavelmente pouco reconhecido. Objetivo: O objetivo deste estudo foi relatar os motivos para solicitar consultas neurológicas por clínicos e intensivistas em um hospital dedicado à COVID-19. Métodos: Estudo retrospectivo realizado no Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brasil, um centro dedicado à COVID-19 com 900 leitos (incluindo 300 leitos para unidades de terapia intensiva). O diagnóstico de COVID-19 foi confirmado por SARS-CoV-2-RT-PCR em swabs nasais. Todas as interconsultas de neurologia hospitalar entre 23 de março e 23 de maio de 2020 foram analisadas. Os neurologistas realizaram o exame neurológico, avaliaram todos os dados disponíveis para diagnosticar a patologia neurológica e solicitaram exames adicionais conforme necessidade. Diagnósticos difíceis foram estabelecidos em reuniões de consenso. Após o diagnóstico, os neurologistas participaram da condução dos casos. Resultados: Foram solicitadas consultas neurológicas para 89 de 1.208 (7,4%) em pacientes internados por COVID-19 durante o período. Os principais diagnósticos neurológicos incluíram: encefalopatia (44,4%), acidente vascular cerebral (16,7%), doenças neurológicas prévias (9,0%), crises epilépticas (9,0%), transtornos neuromusculares (5,6%), outras lesões encefálicas agudas (3,4%) e outros sintomas leves inespecíficos (11,2%). Conclusões: A maioria das consultas neurológicas em um hospital dedicado à COVID-19 foi solicitada para condições graves que poderiam afetar o desfecho clínico. Os médicos na linha de frente devem ser capazes de reconhecer sintomas neurológicos. Os neurologistas são membros importantes da equipe médica no atendimento hospitalar à COVID-19.

Background: More than one-third of COVID-19 patients present neurological symptomsranging from anosmia to stroke and encephalopathy. Furthermore, pre-existingneurological conditions may require special treatment and may be associated with worseoutcomes. Notwithstanding, the role of neurologists in COVID-19 is probablyunderrecognized. Objective: The aim of this study was to report the reasons forrequesting neurological consultations by internists and intensivists in a COVID-19-dedicated hospital. Methods: This retrospective study was carried out at Hospital dasClínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil, a 900-bedCOVID-19 dedicated center (including 300 intensive care unit beds). COVID-19 diagnosiswas confirmed by SARS-CoV-2-RT-PCR in nasal swabs. All inpatient neurologyconsultations between March 23rd and May 23rd, 2020 were analyzed. Neurologistsperformed the neurological exam, assessed all available data to diagnose theneurological condition, and requested additional tests deemed necessary. Difficultdiagnoses were established in consensus meetings. After diagnosis, neurologists wereinvolved in the treatment. Results: Neurological consultations were requested for 89 outof 1,208 (7.4%) inpatient COVID admissions during that period. Main neurologicaldiagnoses included: encephalopathy (44.4%), stroke (16.7%), previous neurologicaldiseases (9.0%), seizures (9.0%), neuromuscular disorders (5.6%), other acute brainlesions (3.4%), and other mild nonspecific symptoms (11.2%). Conclusions: Mostneurological consultations in a COVID-19-dedicated hospital were requested for severeconditions that could have an impact on the outcome. First-line doctors should be able torecognize neurological symptoms; neurologists are important members of the medicalteam in COVID-19 hospital care.

Introdução: Mais de um terço dos pacientes com COVID-19 apresentam sintomasneurológicos que variam de anosmia a AVC e encefalopatia. Além disso, doençasneurológicas prévias podem exigir tratamento especial e estar associadas a pioresdesfechos. Não obstante, o papel dos neurologistas na COVID-19 é provavelmentepouco reconhecido. Objetivo: O objetivo deste estudo foi relatar os motivos para solicitarconsultas neurológicas por clínicos e intensivistas em um hospital dedicado à COVID-19. Métodos: Estudo retrospectivo realizado no Hospital das Clínicas da Faculdade deMedicina da Universidade de São Paulo, Brasil, um centro dedicado à COVID-19 com900 leitos (incluindo 300 leitos para unidades de terapia intensiva). O diagnóstico deCOVID-19 foi confirmado por SARS-CoV-2-RT-PCR em swabs nasais. Todas asinterconsultas de neurologia hospitalar entre 23 de março e 23 de maio de 2020 foramanalisadas. Os neurologistas realizaram o exame neurológico, avaliaram todos os dadosdisponíveis para diagnosticar a patologia neurológica e solicitaram exames adicionaisconforme necessidade. Diagnósticos difíceis foram estabelecidos em reuniões deconsenso. Após o diagnóstico, os neurologistas participaram da condução dos casos.Resultados: Foram solicitadas consultas neurológicas para 89 de 1.208 (7,4%) empacientes internados por COVID-19 durante o período. Os principais diagnósticosneurológicos incluíram: encefalopatia (44,4%), acidente vascular cerebral (16,7%),doenças neurológicas prévias (9,0%), crises epilépticas (9,0%), transtornosneuromusculares (5,6%), outras lesões encefálicas agudas (3,4%) e outros sintomasleves inespecíficos (11,2%). Conclusões: A maioria das consultas neurológicas em umhospital dedicado à COVID-19 foi solicitada para condições graves que poderiam afetaro desfecho clínico. Os médicos na linha de frente devem ser capazes de reconhecersintomas neurológicos. Os neurologistas são membros importantes da equipe médica noatendimento hospitalar à COVID-19.

Abstract Introduction: functional rehabilitation (FR) is a link between healthcare and work demands, and necessarily leads to intervention in the working context. Objective: to present the implementation of FR measures in a federal university from the Southeast region of Brazil. Methods: we described the FR theoretical-methodological definition, the principles included in the current legislation, the criteria and procedures applied to FR as well as its institutional possibilities and limitations. We evaluated the results of the first year of FR implementation, 2016, by analyzing cases of returning to work. Results: FR measures were provided to nine pilot cases. After the first year, there was a reduction of 37% in sick leave, compared to the previous year. Conclusion: it is important to formulate policies and legislation that better define the FR conceptual/epistemological scope and present theoretical/methodological framework for enabling the implementation of FR services in federal universities.

Resumo Introdução: a reabilitação funcional (RF) se apresenta como um elo entre os cuidados de saúde e as demandas do contexto laboral e envolve, necessariamente, intervenções no contexto de trabalho. Objetivo: apresentar a implantação de ações de RF em uma instituição federal de ensino superior (Ifes) da região Sudeste. Métodos: são descritos o processo de definição teórico-metodológico, a problematização dos princípios versados na legislação vigente, os critérios e procedimentos aplicados na RF, as possibilidades e os limites do contexto institucional. São analisados os resultados do programa no primeiro ano de implantação da RF, por meio de avaliação de casos de trabalhadores reinseridos. Resultados: as ações de RF foram oferecidas para nove casos-piloto direcionados para a equipe e distribuídas entre seus membros. No primeiro ano do programa, 2016, após a implantação de ações de RF, foi observada redução de 37% no percentual médio do número de dias de afastamento do trabalho, comparada aos afastamentos do ano anterior. Conclusão: é importante elaborar diretrizes políticas que melhor definam o escopo conceitual e epistemológico da RF, refletidas na legislação, e que apresentem possibilidades teórico-metodológicas para viabilizar a implantação de serviços de RF nas Ifes.

BACKGROUND Lutzomyia longipalpis-derived cell line (Lulo) has been suggested as a model for studies of interaction between sandflies and Leishmania. OBJECTIVES Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with Leishmania (Viannia) braziliensis. METHODS Lulo cell protein extracts were analysed through a combination of two-dimensional gel electrophoresis and mass spectrometry and resulting spots were further investigated in silico to identify proteins using Mascot search and, afterwards, resulting sequences were applied for analysis with VectorBase. RESULTS Sixty-four spots were identified showing similarities to other proteins registered in the databases and could be classified according to their biological function, such as ion-binding proteins (23%), proteases (14%), cytoskeletal proteins (11%) and interactive membrane proteins (9.5%). Effects of interaction with L. (V.) braziliensis with the expression of three genes (enolase, tubulin and vacuolar transport protein) were observed after an eight-hour timeframe and compared to culture without parasites, and demonstrated the impact of parasite interaction with the expression of the following genes: LLOJ000219 (1.69-fold), LLOJ000326 (1.43-fold) and LLOJ006663 (2.41-fold). CONCLUSIONS This set of results adds relevant information regarding the usefulness of the Lulo cell line for studies with Leishmania parasites that indicate variations of protein expression.

Abstract Objectives: to determine the fatty acid composition of mature milk of nursing mothers and its distribution according to some maternal variables. Methods: this is a cross-sectional observational epidemiological study based on the eva-luation of the fatty acid profile of mature human milk. Samples of mature breast milk were taken from 106 nursing mothers, by manual milking and who were after the 5th postpartum week. The milk fat extraction was carried out by using the Bligh and Dyer method and methy-lated with 0.25 mol/L sodium methoxide in methanol diethyl ether. The fatty acid of the milk profile was determined by a Gas Chromatograph equipped with a flame ionization detector. Results: among the saturated fatty acids, the highest values were observed for palmitic (C16:0), stearic (C18:0), myristic (C14:0) and lauric (C12:0) fatty acids, respectively. Among the monounsaturated fatty acids, there was a higher contribution of oleic (C18:1) and palmi-toleic (C16:1) fatty acids, respectively. The total essential fatty acids (linoleic and α-linolenic) was 14.94%. Conclusions: a low content of essential fatty acids in the breast milk of the nursing mothers was observed in the present study, which are important for infant growth and deve-lopment. We suggest the need to implement nutrition education strategies aimed for pregnant women and nursing mothers who should be advised to eat healthier foods.

Resumo Objetivos: determinar a composição em ácidos graxos do leite maduro de nutrizes e sua distribuição segundo algumas variáveis maternas. Métodos: trata-se de um estudo epidemiológico observacional, transversal realizado a partir da avaliação do perfil de ácidos graxos do leite humano maduro. Amostras de leite materno maduro foram obtidas de 106 nutrizes, a partir da 5ª semana pós-parto, por meio de ordenha manual. A extração da gordura do leite foi realizada através do método de Bligh e Dyer, e metiladas com metóxido de sódio 0,25 mol/L em metanol dietil - éter. O perfil de ácidos graxos do leite foi determinado por um Cromatógrafo a Gás equipado com detector por ionização de chamas. Resultados: dentre os ácidos graxos saturados, foram observados valores mais elevados para os ácidos graxos palmítico (C16:0), esteárico (C18:0), mirístico (C14:0) e láurico (C12:0), respectivamente. Entre os ácidos graxos mono-insaturados, verificou-se maior contribuição dos ácidos graxos oleico (C18:1) e palmitoleico (C16:1), respectivamente. O total de ácidos graxos essenciais (linoleico e α- linolênico) foi de 14,94%. Conclusões: foi observado baixo teor de ácidos graxos essenciais no leite materno das nutrizes do presente estudo, que são importantes para o crescimento e desenvolvimento do lactente, sugerindo-se a necessidade de implementação de estratégias de educação nutricional direcionadas a gestantes e nutrizes que devem ser orientadas a consumir alimentos mais saudáveis.

Abstract Melanoacanthoma is a rare variant of seborrheic keratosis, which is notable for dark pigmentation and fast radial growth, making it difficult to distinguish from melanoma. Histologically, it is characterized by proliferation of keratinocytes and dendritic melanocytes. The authors report a scalp lesion, fast growing, suspected by dermoscopy and confocal microscopy examination, with dendritic cells distributed throughout the lesion. Based on these findings, it was not possible to classify this lesion as clearly benign, so it was excised. Histopathologic evaluation and immunostain were consistent with melanoacanthoma.

Abstract Purpose: To analyze quantitative and qualitative data about the profile of the Medical student who attended the Ophthalmology discipline, correlating aspects of the basic knowledge and the student perspectives on his training. Methods: An observational cross-sectional survey study was performed in 242 students over 12 brazilian states. The project was done by Associação Brasileira de Ligas Acadêmicas de Oftalmologia (ABLAO) with the participation of the associated Academic Leagues of Ophthalmology. The sample was composed by random undergraduate medical students, who have already had Ophthalmology as subject. An individual questionnaire was used with questions about the student profile, basic knowledge of the speciality and perspectives about their teaching. Descriptive statistical analysis was performed with the Statistical Package for Social Sciences SPSS program, measuring mean, standard deviation and 95% confidence intervals. Results: The mean age was 23.96 (3.36±) years and female students were predominant in 63.6%. 42% of the states from all regions of Brazil participated. 71.9% of the students were from private educational institutions and were in the 8th period (±1,97). 43% were a member of the Academic League of Ophthalmology in the origin institution. Basic knowledge questions had a variation of the correct answers. Despite of only 31% of the students feel safe to attend or refer patients for evaluation of the specialist, 95.9% of the students answered that they consider ophthalmology important in their training as general practitioner. Conclusion: We defined in this research a profile for the undergraduate student who attended the Ophthalmology Department. We verify how the student considers eye health important and how they search for more knowledge in his preparation in general. We also found that the Medical Academic Leagues make up the main extracurricular support for their learning. It is important to discuss and implement different teaching strategies to improve their training.

Resumo Objetivo: Analisar qualitativa e quantitativamente o perfil do aluno de Medicina que cursou a disciplina de Oftalmologia, correlacionando aspectos do seu ensino em conhecimentos básicos e perspectivas discente sobre a sua formação. Métodos: Foi realizado um estudo transversal descritivo do tipo inquérito, em 242 alunos de 12 estados brasileiros. A execução do projeto foi feita com a participação das Ligas Acadêmicas de Oftalmologia de Instituições de Ensino Superior vinculadas a Associação Brasileira de Ligas Acadêmicas de Oftalmologia (ABLAO). A amostra foi composta por alunos da graduação do curso de Medicina, escolhidos aleatoriamente, que já cursaram uma disciplina referente a Oftalmologia. Foi utilizado um questionário individual com perguntas sobre o perfil do aluno, conhecimentos básicos da especialidade e perspectivas sobre o seu ensino. A análise estatística foi feita com o programa Statistical Package for the Social Sciences SPSS, de forma descritiva, com média, desvio padrão e intervalo de confiança de 95%. Resultados: A média de idade foi de 23,96 (3,36±) anos de idade, e o sexo feminino foi predominante em 63,6%. Participaram 42% dos estados de todas as regiões do Brasil nessa pesquisa. 71,9% dos alunos eram de instituições privadas de ensino e estavam cursando o 8 ° período (±1,97). 43 % faziam parte de Liga Acadêmica de Oftalmologia. Observou-se variação de acertos nas questões de conhecimentos básicos, e ao final 95,9% dos alunos responderam que consideram a Oftalmologia importante na sua formação como médico geral, entretanto apenas 31% se sentem seguros em atender e ou encaminhar pacientes para avaliação do especialista. Conclusão: Definimos nessa pesquisa um perfil para o aluno de graduação que cursou a Disciplina de Oftalmologia. Verificamos o quanto ele considera importante a saúde ocular, e que existe uma procura por mais conhecimento no seu preparo como médico generalista. Constatamos também que as Ligas Acadêmicas de Medicina compõem o principal apoio extracurricular ao seu aprendizado. Consideramos importante que diferentes estratégias de ensino sejam discutidas e implementadas para melhora na sua formação.

Abstract Introduction: accidental dislodgement of enteral feeding tubes has been considered as an important quality indicator of the efficacy of enteral nutrition therapy. However, in clinical practice, the use of feeding tube attachment devices (FTADs), as an alternative to the traditional method of adhesive tape alone, has not yet been evaluated for its effectiveness in reducing inadvertent tube dislodgement. Objective: to evaluate the impact of using a dedicated FTAD compared with the traditional securing method with adhesive tape on the occurrence of accidental enteral feeding tube removal. Methods: a randomized clinical trial comparing two strategies for enteral feeding tube securement: use of traditional adhesive tape vs FTAD. The primary endpoint was the percentage of accidental enteral feeding tube dislodgement after randomization. Results: a total of 104 inpatients (mean age: 61.4 ± 17.5 years) were included (52 patients per group). Most were women with cerebrovascular disease (35.6%), diabetes (28.8%) and neoplasia (27.9%). There were 39 (37.5%) cases of accidental tube removal, 30.8% in the FTAD group and 44.2% in the adhesive tape group (p = 0.22). During follow-up, patients in the FTAD group received a mean of 60.0% of the volume of enteral nutrition prescribed, while patients in the adhesive tape group received 57.0% (p = 0.61). There was no difference in skin lesions between the groups. Conclusion: the strategy of using a dedicated FTAD as the method for securing enteral feeding tubes did not reduce the risk of accidental tube dislodgement compared with the traditional securing method with adhesive tape.

Resumen Introducción: la expulsión accidental de sondas de alimentación enteral se ha considerado un indicador importante de la calidad de la eficacia de la terapia de nutrición enteral. Sin embargo, en la práctica clínica, el uso de dispositivos de fijación de tubos de alimentación (FTAD, por sus siglas en inglés), como una alternativa al método tradicional de cinta adhesiva exclusivamente, aún no se ha evaluado por su eficacia para reducir el desprendimiento accidental de sondas. Objetivo: evaluar el impacto de usar un FTAD dedicado en comparación con el método tradicional de aseguramiento con cinta adhesiva en caso de que se produzca una extracción accidental de la sonda de alimentación enteral. Métodos: se realizó un ensayo clínico aleatorizado que comparó dos estrategias para asegurar la sonda de alimentación enteral: el uso de cinta adhesiva tradicional frente a FTAD. El punto final primario fue el porcentaje de desplazamiento accidental del tubo de alimentación enteral después de la aleatorización. Resultados: se incluyó un total de 104 pacientes hospitalizados (edad media 61,4 ± 17,5 años) (52 pacientes por grupo). La mayoría eran mujeres con enfermedad cerebrovascular (35,6%), diabetes (28,8%) y neoplasia (27,9%). Hubo 39 casos (37.5%) de extracción accidental de tubos, 30,8% en el grupo FTAD y 44,2% en el grupo de cinta adhesiva (p = 0,22). Durante el seguimiento, los pacientes del grupo FTAD recibieron una media del 60,0% del volumen de nutrición enteral prescrito, mientras que los pacientes del grupo de cinta adhesiva recibieron el 57,0% (p = 0,61). No hubo diferencia en las lesiones de la piel entre los grupos. Conclusión: la estrategia de utilizar un FTAD dedicado como método para asegurar las sondas de alimentación enteral no redujo el riesgo de retiradas accidentales en comparación con el método tradicional de sujeción con cinta adhesiva.

Given the idiosyncrasies of call center work, its impact on the lives of employees and their possible reactions, we analyzed what forms of retaliation can occur in such an environment by considering gender. We performed qualitative descriptive research. We have interviewed 20 call center agents (9 men and 11 women) in Belo Horizonte, the capital city of Minas Gerais state, in Brazil. We fully transcribed all interviews and subjected the data to discourse analysis (the French approach). The results showed that in this case, there was no gender difference regarding perceived unfairness. Thus, workers of both genders often retaliate and consider their actions to be reasonable.

Dadas as idiossincrasias do trabalho do call center, seu impacto na vida dos funcionários e suas possíveis reações, analisamos quais formas de retaliação podem ocorrer em um ambiente desse tipo, considerando gênero. Realizamos pesquisa descritiva qualitativa. Entrevistamos 20 agentes do call center (9 homens e 11 mulheres) em Belo Horizonte, capital do estado de Minas Gerais, no Brasil. Todas as entrevistas foram transcritas na íntegra e os dados foram submetidos à análise do discurso (abordagem francesa). Os resultados mostraram que, neste caso, não houve diferença de gênero em relação às injustiças percebidas. Assim, os trabalhadores de ambos os sexos muitas vezes retaliam e consideram suas ações razoáveis.

BACKGROUND A number of Zika virus (ZIKV) sequences were obtained using Next-generation sequencing (NGS), a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences. OBJECTIVE The aim of this study was to develop a simple and robust Sanger sequencing protocol targeting ZIKV relevant genetic regions, as envelope protein and nonstructural protein 5 (NS5). In addition, phylogenetic analysis of the ZIKV strains obtained using the present protocol and their comparison with previously published NGS sequences were also carried out. METHODS Six Vero cells isolates from serum and one urine sample were available to develop the procedure. Primer sets were designed in order to conduct a nested RT-PCR and a Sanger sequencing protocols. Bayesian analysis was used to infer phylogenetic relationships. FINDINGS Seven complete ZIKV envelope protein (1,571 kb) and six partial NS5 (0,798 Kb) were obtained using the protocol, with no amplification of NS5 gene from urine sample. Two NS5 sequences presented ambiguities at positions 495 and 196. Nucleotide analysis of a Sanger sequence and consensus sequence of previously NGS study revealed 100% identity. ZIKV strains described here clustered within the Asian lineage. MAIN CONCLUSIONS The present study provided a simple and low-cost Sanger protocol to sequence relevant genes of the ZIKV genome. The identity of Sanger generated sequences with published consensus NGS support the use of Sanger method for ZIKV population studies. The regions evaluated were able to provide robust phylogenetic signals and may be used to conduct molecular epidemiological studies and monitor viral evolution.

INTRODUCCIÓN: Pocos estudios han correlacionado las medidas de equilibrio entre las principales pruebas funcionales y la plataforma de fuerza en los atletas.OBJETIVO: Determinar la relación entre tres pruebas de equilibrio funcionales con las principales medidas de la fuerza en la plataforma de los atletas.MÉTODO: Quince atletas del sexo femenino que practican el fútbol de salón y balonmano, entre 13 y 17 años participaron en el estudio. Los atletas realizaron tres pruebas funcionales: 1) Star excursion balance test, 2) salto lateral y 3) salto figura ocho, además de una prueba unipodal sobre una plataforma de fuerza con los ojos abiertos durante 30 segundos. Los parámetros del centro de la presión de los pies (COP) se calcularon por análisis estabilográfica. Para todas las pruebas (funcionales y plataforma), se llevaron a cabo tres ensayos y el promedio fue utilizado para su análisis.RESULTADOS: Las correlaciones entre las pruebas funcionales y la plataforma de fuerza fueron r = 0,01 a -0,69 (leve a moderada), en función de la variable COP. La mejor correlación (r = -0,69) se encontró entre el rango de la prueba Star Excursion Balance y el área del COP, lo que indica que cuanto mayor es la distancia que se logra, mejor es el equilibrio. En segundo lugar, se encontró correlación débil a moderada entre la prueba en figura de ocho y el área del COP (por ejemplo, r = 0,43). La prueba de salto lateral mostró una buena correlación con las pruebas en la plataforma de fuerza.CONCLUSIÓN: Este estudio demostró que para la evaluación del equilibrio de los atletas del sexo femenino de fútbol de salón y balonmano, el Star excursion balance test se puede utilizar en la ausencia de una plataforma de fuerza para identificar posibles deficiencias en el equilibrio.

INTRODUCTION: Few studies have correlated the balance measurements based in functional tests and force platform in athletes.OBJECTIVE: to determine the relationship between three functional balance tests with the main measurements of the force platform in athletes.METHODOLOGY: Fifteen female athletes, indoor soccer and handball players, aged between 13 and 17 years, participated of this study. The athletes performed tree functional tests: 1) the star excursion balance test, 2) the lateral jump, and 3) the figure eight test; as well as the one leg-stance test on a force platform with the eyes open for 30 seconds. The parameters of center of pressure (COP) of the foot were calculated by stabilographic analysis. For all the tests (functional and platform), tree trials attempts were made, and the mean value retained for the analyses.RESULTS: The correlations between the functional tests and the force platform were from r = 0.01 to -0.69 (weak to moderate), depending on the COP variable. The best correlations (r = -0.69) were found between the star excursion balance test and the COP area, indicating that when a greater distance was reached, the better the postural balance. Secondly, a weak to moderate correlation was found between the figure eight test and COP area (e.g. r = 0.43). The side hop test did not present good correlation with the values obtained on the force platform.CONCLUSION: This study shows that to evaluate balance in athletes practicing indoor soccer and handball, the Star excursion balance test may be used in the absence of a force platform, to identify possible balance deficits.

INTRODUÇÃO: Poucos estudos correlacionaram as medidas de equilíbrio entre os principais testes funcionais e a plataforma de força em atletas.OBJETIVO: Determinar a relação entre três testes funcionais de equilíbrio com as principais medidas da plataforma de força em atletas.MÉTODO: Quinze atletas do sexo feminino praticantes de futebol de salão (futsal) e handebol, com idades entre 13 a 17 anos participaram do estudo. As atletas realizaram três testes funcionais: 1) Star excursion balance test, 2) Salto lateral e 3) Figura em oito; além de um teste em apoio unipodal sobre uma plataforma de força com os olhos abertos durante 30 segundos. Os parâmetros do centro de pressão dos pés (COP) foram calculados por análise estabilográfica. Para todos os testes (funcionais e plataforma), três tentativas foram realizadas e a média foi retida para as análises.RESULTADOS: As correlações entre os testes funcionais e a plataforma de força foram de r = 0,01 a -0,69 (fraca a moderada), dependendo da variável do COP. As melhores correlações (r = -0,69) foram encontradas entre o Star Excursion Balance Test de alcance e a área do COP, indicando que quanto maior a distância alcançada, melhor o equilíbrio. Segundo, uma correlação de fraca a moderada foi encontrada entre o teste da figura em oito e a área do COP (p. ex: r= 0.43). O teste de salto lateral não apresentou boa correlação com os obtidos na plataforma de força.CONCLUSÃO: Este estudo demonstrou que para a avaliação do equilíbrio em atletas de futsal e handebol feminino o teste Star Excursion Balance Test pode ser usados na ausência da plataforma de força para identificação de possíveis déficits de equilíbrio.