Resumo Este estudo foi conduzido para avaliar a poluição da água examinando a fragmentação do DNA em órgãos de peixes selecionados (rim, fígado, brânquias e tecido muscular) de Wallago attu, Sperata sarwari, Vulgaris vulgaris e Labeo rohita coletados de uma conhecida área poluída do rio Chenab, Paquistão e de um local de controle. Os peixes foram capturados usando uma rede branquial e foram divididos em três grupos de pesos diferentes (W1, W2 e W3) para estudar o grau de variação na fragmentação do DNA em relação ao peso corporal. Nos peixes do local poluído, a fragmentação do DNA foi maior nos rins, fígado, brânquias e músculos, em comparação ao controle. Não foi observada fragmentação significativa do DNA em peixes coletados no local de controle. Relação altamente significativa (P <0,01) entre o peso corporal e a fragmentação do DNA foi encontrada nos órgãos dos peixes adquiridos no local contaminado. Verificou-se que a fragmentação do DNA nos órgãos do corpo é afetada pelas concentrações de chumbo, cobre, níquel e cádmio em W. attu, S. sarwari, L. rohita e V. vulgarus colhidos no rio Chenab. A fragmentação do DNA em diferentes espécies de peixes de água doce é, portanto, um biomarcador confiável da poluição da água.

Abstract This study was conducted to assess water pollution by examining DNA fragmentation in selected fish organs (kidney, liver, gills, and muscle tissue) from Wallago attu, Sperata sarwari, Vulgaris vulgaris, and Labeo rohita collected from a known polluted section of the Chenab River, Pakistan, and from a control site. The fish were caught using a gill net and were assigned to three different weight groups (W1, W2, and W3) to study the degree of variation in DNA fragmentation in relation to body weight. In fish from the polluted site, DNA fragmentation was higher in kidney, liver, gills, and muscles, compared to the control. No significant DNA fragmentation was observed in fish collected from the control site. Highly significant (P < 0.01) relationship between body weight and DNA fragmentation was found in the organs of fish procured at the contaminated site. DNA fragmentation in body organs was found to be affected by the concentrations of lead, copper, nickel, and cadmium in W. attu, S. sarwari, L. rohita, and V. vulgarus harvested from Chenab River. DNA fragmentation in different freshwater fish species is therefore a reliable biomarker of water pollution.

RESUMO: Em arroz semeado direto (DSR), são encontradas descargas de plantas daninhas, e a maioria dos herbicidas subsequentes precisam se aplicar para controlar as plantas daninhas que aparecem mais tarde. Uma abordagem amigável ao agricultor foi hipotetizada no presente estudo para integrar as estratégias de manejo de água, solo e produção de arroz sem ervas econômicas. Foi avaliada a eficácia de vários herbicidas no controle de plantas daninhas para melhorar o desempenho do arroz aeróbio cultivado em lavoura variável e regimes alternativos de água. Foram realizadas cinco técnicas de manejo de plantas daninhas (controle de plantas daninhas, sem plantas daninhas, pendimetalina, seguidas de remoção manual, pendimethalin + bispyribac sodium (BS) + bensulfuron (B) e pendimethalin + MCPA) nos regimes alternativos de molhagem e secagem (AWD) (15 E 20 cm de profundidade) sob dois sistemas de preparo do solo (zero e convencional). Todos os tratamentos de herbicidas reduziram a intensidade da planta daninha em comparação com a verificação de plantas daninhas, porém a densidade mínima de plantas daninhas e a biomassa seca foram observadas na pendimetalina aos 40 e 60 dias após a semeadura, respectivamente. Sob plantio convencional, as parcelas sem plantas apresentaram índice máximo de área foliar, duração da área foliar e taxa de crescimento da cultura. A altura máxima da planta, o peso do milho e a semente por panícula, juntamente com menos porcentagem de grãos abortivos, calcários e opacos, foram registrados em pendimetalina, seguido de BS + B, em comparação com parcelas de verificação de plantas daninhas. Entre a aplicação de herbicidas, a pendimetalina, seguida de BS + B, proporcionou maior benefício em relação custo e benefícios líquidos em ambos os regimes AWD. Em geral, a aplicação de pendimetalina seguida de BS + B é uma abordagem eficiente e econômica para reduzir a infestação de plantas daninhas, o que resulta em melhor produção de arroz aeróbio.

ABSTRACT: In direct seeded rice (DSR), flushes of weeds are encountered, and mostly subsequent herbicides need to apply to control weeds that appear later. A farmer friendly approach was hypothesized in present study to integrate the management strategies for water, soil and cost effective weed free rice production. Efficacy of various herbicides in weed control to improve the performance of aerobic rice grown under varying tillage and alternate water regimes was evaluated. Five weed management techniques (weedy check, weed-free, pendimethalin followed by manual weeding, pendimethalin + bispyribac sodium (BS) + bensulfuron (B) and pendimethalin + MCPA) were carried out within the alternate wetting and drying (AWD) regimes (15 and 20 cm depth) under two tillage systems (zero and conventional). All the herbicide treatments reduced the weed intensity as compared to weedy check, however minimum weed density and dry biomass was observed in pendimethalin at 40 and 60 days after sowing, respectively. Under conventional tillage, weed free plots showed maximum leaf area index, leaf area duration and crop growth rate. Maximum plant height, 1000-kernel weight and kernel per panicle along with less percentage of abortive, chalky and opaque kernels were recorded in pendimethalin followed by BS+B as compared to weedy check plots. Among the herbicides application, pendimethalin followed by BS+B gave highest benefit to cost ratio and net benefits under both AWD regimes. Overall, application of pendimethalin followed by BS+B is an efficient and economic approach to reduce weed infestation which results in improved yield of aerobic rice.

ABSTRACT: The present study was aimed to evaluate the effect of heavy metals on an important tissue of two fish species Cyprinus carpio and Wallago attu, sampled from the Indus river, Mianwali District, Pakistan. The concentration of selected heavy metals Fe, Cr, Cu, and in gills, muscles, kidney and liver was compared with an International standard of food fish. The overall metal concentrations among different weight categories in C. carpio were in the order of Fe > Cu > Cr >. In W. attu the overall accumulation of these metals were, in order of Fe > Cu > Cr > Pb The order of accumulation of metals in gills and muscle of C. carpio was Fe > Cr > Pb > Cu; kidney and muscles of W. attu was Fe > Cr > Cu > Pb; liver Fe > Cu > Cr > Pb. An increasing trend of concentration of iron, copper, chromium and lead occurred with an increase in weight of C. carpio and W. attu. There was a significant difference in the accumulation of heavy metals in different organs of both species (p<0.01). All studied heavy metals except Cr were within permissible limits described by various international agencies like WHO, FAO and FEPA in edible tissues of C. carpio and W. attu.