Abstract Inflammatory bowel disease (IBD) is a problem that directly affects the quality of life of patients suffering from this condition. Monitoring the serum level of infliximab (IFX) (TDM) is an important tool for guiding therapeutic decisions in IBD patients. The purpose of this study was to determine the significance of quantitatively measuring the serum level of IFX (TDM) and antibody to IFX (ATI). Methods and materials: Prospective observational study involving 40 IBD patients on IFX therapy, including 14 Proactive (week 06 of the induction phase) and 26 Reactive (maintenance phase). Immediately prior to the infusion, blood samples were drawn and measured using a Bulhlmann rapid test instrument. Serum concentrations of IFX were categorized as supratherapeutic (>7.0 micrograms/ml), therapeutic (between 3.0 and 7.0 micrograms/ml), and subtherapeutic (3.0 micrograms/ml). When the serum concentration of IFX was 3 mcg/ml (subtherapeutic), the ATI was measured. 25 patients with CD and 15 patients with UC were evaluated. Only three of the twenty patients with subtherapeutic serum levels had a positive ATI, and both were reactive; two had CD and one had UC. There was a statistically significant difference between reactive and proactive patients with respect to levels of CRP (p = 0.042), with proactive DNS patients suffering greater alterations in CRP and albumin.

ABSTRACT Tifton 85 bermudagrass has been widely used in tropical and subtropical regions. Owing to its high forage yield, the fertilizer requirement of Tifton 85 is also high. Inoculation with Azospirillum brasilense is an alternative to reduce the use of nitrogen fertilizers and decrease their environmental impact. However, the effects of inoculation in tropical grasses remain poorly understood. This study aimed to evaluate the productivity, pasture characteristics, and nutritive value of Tifton 85 bermudagrass inoculated with A. brasilense in association with nitrogen fertilization. The study evaluated four nitrogen levels (0, 100, 200, and 300 kg ha-1 ), inoculated and uninoculated, under cutting conditions. The experimental design was completely randomized, in a factorial arrangement, with two qualitative (inoculated with A. brasilense or uninoculated) and four quantitative levels (nitrogen doses) with three replications (24 plots). Botanical composition and morphological characteristics, leaf blade/stem+sheath ratio, nutritive value, and forage yield were evaluated. The inoculation increased the leaf blade/stem+sheath ratio and forage yield of Tifton 85, but did not affect crude protein concentration, neutral detergent fiber content, and total digestible nutrient content of the forage constituted by Tifton 85 leaf blades. The protein concentration and forage production corresponded linearly to the nitrogen dose increase.

ABSTRACT OBJECTIVE To update the estimated cost of physical inactivity for the Brazilian Unified Health System (SUS). METHODS The hospitalization costs were accessed via a database of the Ministry of Health – Informatics Department of the Brazilian SUS. Physical inactivity for the year 2017 was accessed via the Sistema de Vigilância de Fatores de Risco e Proteção para Doenças Crônicas por Inquérito Telefônico (Vigitel – Surveillance System for Risk and Protective Factors for Chronic Diseases by Telephone Survey). Seven chronic non-communicable diseases (NCD) were selected via the international classification of disease (ICD-10). The population fraction attributable to physical inactivity was calculated based on relative risk reported in previous studies and the prevalence of physical inactivity. RESULTS In 2017, the seven NCD considered in the analysis were responsible for 154,017 hospital admissions in adults older than 40 years old, residing in the state capitals and the Federal District, which corresponded to 6.5% of hospitalizations and 10.6% of SUS costs at an estimated US$ 112,524,914.47. Considering the group of individuals with insufficient physical activity in their leisure time, the percentage cost attributed to physical inactivity reached 17.4% of the estimated costs with NCD. At a national level, NCD were responsible for approximately 740 thousand hospitalizations, costing US$ 482 million, from which 17.4%, US$ 83 million were attributed to physical inactivity. CONCLUSION This study provides evidence to conclude that physical inactivity exerts an economic impact on the SUS due to NCD hospitalization. Physical inactivity is a modifiable lifestyle and compelling evidence, including that of this article, supports the promotion of a more active community as one of the major targets of public health care policies.

Abstract Preterm birth (PTB) is the main condition related to perinatal morbimortality worldwide. The aim of this study was to identify gene-environment interactions associated with spontaneous PTB or its predictors. We carried out a retrospective case-control study including parental sociodemographic and obstetric data as well as newborn genetic variants of 69 preterm and 61 at term newborns born at a maternity hospital from Tucumán, Argentina, between 2005 and 2010. A data-driven Bayesian network including the main PTB predictors was created where we identified gene-environment interactions. We used logistic regressions to calculate the odds ratios and confidence intervals of the interactions. From the main PTB predictors (nine exposures and six genetic variants) we identified an interaction between low neighbourhood socioeconomic status and rs2074351 (PON1, genotype GG) variant that was associated with an increased risk of toxoplasmosis (odds ratio 12.51, confidence interval 95%: 1.71 - 91.36). The results of this exploratory study suggest that structural social disparities could influence the PTB risk by increasing the frequency of exposures that potentiate the risk associated with individual characteristics such as genetic traits. Future studies with larger sample sizes are necessary to confirm these findings.

Abstract Lipids extracted from microalgae for biofuel production have drawn the attention of researchers. Various methods for quantification of neutral lipids, such as solvent extraction or gravimetric, require a large volume of sample and are time-consuming. In this work, Nile Red was employed to evaluate the lipid content in microalgae Scenedesmus sp. through 2D fluorescence spectroscopy using chemometric modeling with a selection of wavelength pairs reducing the variable numbers. Cultivation of microalgae for 10 days was performed. Samples were taken starting after the 5th day of cultivation. Microalgae samples were dried using an oven with tangential airflow and the conventional Bligh and Dyer method for lipid extraction was employed. Before the measurement of fluorescence, the samples were diluted in ethanol at 40°C for 10 minutes, and then Nile Red solution (10 µg/mL ethanol) was added. The results of lipid quantification using the proposed technique achieved mean absolute percentage error (MAPE) lower than 5% and correlation coefficients around 0.99 in the test phase. Linear models using four excitation-emission pairs of fluorescence were fitted and were selected based on Pure Spectral Chemometric Modelling (PSCM). The method for lipid quantification using predictive linear models and 2D fluorescence proved to be robust and fast (requiring less than 30 min) compared to the conventional gravimetric method (which takes at least 5 hours) to obtain comparable results.

ABSTRACT. The use of multispectral images has great potential to assess seed quality and represents a significant technological advance in the search for fast and non-destructive analysis techniques. However, the devices currently available are expensive. Thus, this study aimed to propose a low-cost method for acquisition and processing of multispectral images of soybean seeds and to evaluate their potential for rapid determination of seed physiological potential. The study was conducted in three steps: implementation of the multispectral image acquisition system, development of an algorithm for automatic image processing, and evaluation of the relationship between the data obtained through image analysis and the results of standard tests used to evaluate seed physiological potential. A total of 43 variables were assessed, eight related to seed physiological potential (germination and vigor) and 35 obtained from the analysis of the multispectral images. Of the variables obtained from multispectral images, 21 were related to pixel values in the images in the different bands evaluated (green, red, and infrared) and 14 associated with seed morphometric characteristics. The proposed system is efficient in obtaining multispectral images and the algorithm developed was efficient to extract morphometric characteristics and pixel information from the images. The parameters obtained from the NIR spectrum region showed a good relationship with the physiological potential of soybean seeds.

ABSTRACT Objective: to analyze the influence of emotional appeals on behavioral intention regarding organic products for personal care. Theoretical framework: this article uses the literature of emotional appeals, specifically the appeals of guilt, fear, authentic pride, and hubristic pride, to identify their effects on consumers’ behavioral intention in the context of organic personal care products. Emotional appeals of guilt, fear, authentic pride, and hubristic pride were examined. Methodology: four sequential experiments were conducted in real advertisement settings using a within-subjects and between-subjects experimental design. Data were analyzed using ANOVA. Results: the appeal of authentic pride was associated with a heightened value of joy and increased likelihood of consumer donation. The appeal of hubristic pride stimulated an effect of authentic pride. This way, the findings indicate that pride is a one-dimensional emotion in the national context of organic cosmetics consumers. Additionally, the appeal of one-dimensional pride aroused a more positive attitude and purchase intention regarding the advertised product compared to the emotions guilt, fear, and joy. Conclusion: these findings reposition the discussion on pride from a one-dimensional perspective because authentic pride stimulates joy. Further, positive appeals were more effective than negative appeals at increasing the consumers’ behavioral intention toward organic cosmetics. Contrary to the findings of previous surveys, pride was associated with more positive consumer attitudes toward the advertised product. Therefore, the use of pride appeal should be encouraged to promote consumer awareness.

RESUMO Objetivo: analisar a influência de apelos emocionais na intenção comportamental com relação a produtos orgânicos para cuidados pessoais. Marco teórico: utilizou-se a literatura dos apelos emocionais, em específico os apelos de culpa, medo, orgulho autêntico e orgulho hubrístico, para identificar seus efeitos na intenção comportamental no contexto dos produtos orgânicos para cuidados pessoais. Desta forma, utilizaram-se os apelos de culpa, medo, orgulho autêntico e orgulho hubrístico. Metodologia: quatro experimentos sequenciais em configurações de anúncios reais, com desenho experimental (within-subjects e between-subjects), foram realizados. Os dados foram analisados mediante ANOVA. Resultados: o apelo de orgulho autêntico estimulou maior valor de alegria, além de aumentar a probabilidade de os consumidores doarem, enquanto o apelo de orgulho hubrístico estimulou maior efeito de orgulho autêntico. Desta forma, foi evidenciado que o orgulho é uma emoção unidimensional no contexto nacional do consumidor de cosméticos orgânicos. Em seguida, descobriu-se que o apelo de orgulho unidimensional despertou uma maior atitude favorável e intenção de compra do produto anunciado em comparação às emoções culpa, medo e alegria. Conclusão: os achados reposicionam a discussão sobre o orgulho sob uma perspectiva unidimensional, pois o orgulho autêntico estimulou alegria. Ademais, os apelos positivos são mais eficazes para aumentar a intenção comportamental de cosméticos orgânicos. Em divergência com pesquisas anteriores, o orgulho provoca maiores atitudes favoráveis em relação ao produto anunciado. Portanto, para a conscientização do consumidor deve-se incentivar o uso de apelo de orgulho.

ABSTRACT OBJECTIVE To update the estimated cost of physical inactivity for the Brazilian Unified Health System (SUS). METHODS The hospitalization costs were accessed via a database of the Ministry of Health – Informatics Department of the Brazilian SUS. Physical inactivity for the year 2017 was accessed via the Sistema de Vigilância de Fatores de Risco e Proteção para Doenças Crônicas por Inquérito Telefônico (Vigitel – Surveillance System for Risk and Protective Factors for Chronic Diseases by Telephone Survey). Seven chronic non-communicable diseases (NCD) were selected via the international classification of disease (ICD-10). The population fraction attributable to physical inactivity was calculated based on relative risk reported in previous studies and the prevalence of physical inactivity. RESULTS In 2017, the seven NCD considered in the analysis were responsible for 154,017 hospital admissions in adults older than 40 years old, residing in the state capitals and the Federal District, which corresponded to 6.5% of hospitalizations and 10.6% of SUS costs at an estimated US$ 112,524,914.47. Considering the group of individuals with insufficient physical activity in their leisure time, the percentage cost attributed to physical inactivity reached 17.4% of the estimated costs with NCD. At a national level, NCD were responsible for approximately 740 thousand hospitalizations, costing US$ 482 million, from which 17.4%, US$ 83 million were attributed to physical inactivity. CONCLUSION This study provides evidence to conclude that physical inactivity exerts an economic impact on the SUS due to NCD hospitalization. Physical inactivity is a modifiable lifestyle and compelling evidence, including that of this article, supports the promotion of a more active community as one of the major targets of public health care policies.

ABSTRACT Objective To assess the predictive value of preoperative serum laboratory test results for identifying choledocholithiasis and reduce the use of cholangioresonance and its inherent costs. Methods Patients aged 21-69 years who underwent preoperative cholangioresonance examination at our institute were included. Patients with a history of fluctuating jaundice or biliary pancreatitis, bile duct dilatation on ultrasonography, and elevated levels of canalicular enzymes (alkaline phosphatase >100U/L and gamma-glutamyl transferase >50U/L) underwent cholangioresonance-guided surgery. Cases of choledocholithiasis confirmed by cholangioresonance were compared with those without choledocholithiasis. Serum laboratory data were evaluated and the diagnostic capabilities of these examinations were analyzed. Results A total of 104 patients were included. For detecting choledocholithiasis using alkaline phosphatase, the cut-off point was 78U/L, sensitivity was 97.6% (95%CI: 87.4-99.9), and specificity was 72.6% (95%CI: 59.8-83.1). In the binary logistic regression analysis, age (OR= 0.92; 95%CI: 0.86-0.98) and alkaline phosphatase level (OR= 1.02; 95%CI: 1.01-1.05) were selected for the final model. Conclusion Serum alkaline phosphatase levels may aid preoperative diagnosis of asymptomatic choledocholithiasis. After a global clinical assessment of the patient, serum laboratory findings may contribute to a reduction in cholangioresonance-related heathcare costs.

Abstract Background The high incidence of atrial arrhythmias in pulmonary hypertension (PH) might be associated with poor prognosis, and the left atrium (LA) may play a role in this. An important finding in PH studies is that LA remodeling is underestimated. Objective This study investigated LA morphology and mechanical function, as well as the susceptibility to develop arrhythmias in a monocrotaline-induced PH (MCT-PH) model. Methods Wistar rats aged 4 weeks received 50 mg/kg of MCT. Electrocardiography and histology analysis were performed to evaluate the establishment of the MCT-PH model. The tissue was mounted in an isolated organ bath to characterize the LA mechanical function Results Compared with the control group (CTRL), the MCT-PH model presented LA hypertrophy and changes in cardiac electrical activity, as evidenced by increased P wave duration, PR and QT interval in MCT-PH rats. In LA isolated from MCT-PH rats, no alteration in inotropism was observed; however, the time to peak contraction was delayed in the experimental MCT-PH group. Finally, there was no difference in arrhythmia susceptibility of LA from MCT-PH animals after the burst pacing protocol. Conclusion The morphofunctional remodeling of the LA did not lead to increased susceptibility to ex vivo arrhythmia after application of the burst pacing protocol.

Resumo Fundamento A alta incidência de arritmias atriais na hipertensão pulmonar (HP) pode estar associada a um prognóstico ruim, e o átrio esquerdo (AE) pode desempenhar um papel neste quadro. Um achado importante nos estudos de HP é que a remodelação do AE é subestimada. Objetivo Este estudo investigou a morfologia e a função mecânica do AE, bem como a suscetibilidade ao desenvolvimento de arritmias em um modelo de HP induzida por monocrotalina (HP-MCT). Métodos Ratos Wistar com 4 semanas de idade receberam 50 mg/kg de MCT. Foram realizadas análises eletrocardiográficas e histológicas para avaliar o estabelecimento do modelo de HP-MCT. O tecido foi montado em banho de órgão isolado para caracterizar a função mecânica do AE. Resultados Em comparação com o grupo controle, o modelo de HP-MCT apresentou hipertrofia do AE e alterações da atividade elétrica cardíaca, conforme evidenciadas pelo aumento da duração da onda P, PR e intervalo QT. Não foi observada alteração no inotropismo do AE isolado de ratos com HP-MCT; no entanto, o tempo para atingir a contração máxima foi atrasado. Finalmente, não observamos diferença na suscetibilidade à arritmia no AE dos ratos com HP-MCT após o protocolo de estimulação intermitente. Conclusão A remodelação morfofuncional do AE não levou ao aumento da suscetibilidade à arritmia ex vivo após a aplicação do protocolo de estimulação intermitente.

Abstract We compared sampling techniques in a managed native forest in Paragominas, Pará, Brazil. Our goal in this study was to evaluate the feasibility of using Double Sampling (DS) and Sampling with Partial Replacement (SPR), when compared to Continuous Forest Inventory (CFI), to estimate the wood stock for trees with DBH ≥ 20 cm in a managed forest. In our results, DS had the best volume prediction, generating a sampling error of 5.20% (11.48 m³ ha-1) on the second occasion 3.86% (8.78 m³ ha-1). The average volume increment, estimated for the forest in the monitored period (2014-2016) was 6.88 m³ ha-1, with a relative sampling error of 63.09%. Therefore, as an alternative and of low cost, we suggest using DS in successive forest inventories in monitoring areas of forest resources in the Brazilian Amazon.

ABSTRACT Objective: To analyze the prevalence and factors associated with the lifestyle profile of public basic education teachers in Minas Gerais during the COVID-19 pandemic. Method: Epidemiological websurvey carried out with public basic education teachers in Minas Gerais. Data collection took place from August to September2020 via digital form. Anthropometric, sociodemographic, work, and lifestyle characteristics were evaluated. Poisson Regression was used. Results: 15,641 teachers participated and 31.1% had inadequate health habits. There was a higher prevalence among men (PR=1.38; 95%CI:1.31;1.45), older age (PR=1.20; 95%CI: 1.07;1.34), greater weekly workload (RP=1.10; 95%CI:1.03;1.17) and those dissatisfied with work (RP=1.21; 95%CI:1.15;1.27). As a protective factor, professors with longer teaching experience (RP=0.92; 95%CI:0.87;0.98) and those hired or appointed (PR=0.89; 95%CI:0.85) ;0.94). Conclusion: Lifestyle inadequacy was found among older male teachers, with longer working hours and job dissatisfaction.

RESUMEN Objetivo: Analizar la prevalencia y los factores asociados al perfil de estilo de vida de los docentes de educación básica pública de Minas Gerais durante la pandemia de COVID-19. Método: Encuesta epidemiológica de tipo websurvey realizada con profesores de educación básica pública en Minas Gerais. La recolección de datos ocurrió de agosto a septiembre2020 a través de formulario digital. Se evaluaron características antropométricas, sociodemográficas, laborales y de estilo de vida. Se utilizó la Regresión de Poisson. Resultados: Participaron 15.641 docentes y el 31,1% presentaba hábitos de salud inadecuados. Hubo mayor prevalencia en hombres (RP=1,38; IC 95%: 1,31; 1,45), mayor edad (RP=1,20; IC 95%: 1,07; 1,34), mayor carga de trabajo semanal (RP=1,10; IC 95%: 1,03). ;1,17) y los insatisfechos con el trabajo (RP=1,21; IC95%:1,15;1,27). Como factor protector, profesores con mayor experiencia docente (RP=0,92; IC95%:0,87;0,98) y contratados o nombrados (RP=0,89; IC95%:0,85;0,94). Conclusión: Se encontró inadecuación en el estilo de vida entre los maestros hombres y mayores, con jornadas laborales más largas e insatisfacción laboral.

RESUMO Objetivo: Analisar a prevalência e fatores associados ao perfil do estilo de vida dos professores da educação básica pública de Minas Gerais na pandemia da COVID-19. Método: Inquérito epidemiológico websurvey realizado com professores da educação básica pública de Minas Gerais. A coleta de dados ocorreu de agosto a setembro/2020 via formulário digital. Foram avaliadas as características antropométricas, sociodemográficas, laborais e estilo de vida. Utilizou-se a Regressão de Poisson. Resultados: Participaram 15.641 professores e 31,1% apresentaram hábitos inadequados de saúde. Houve maior prevalência entre homens (RP=1,38; IC95%:1,31;1,45), maior idade (RP=1,20; IC95%: 1,07;1,34), maior carga semanal de trabalho (RP=1,10; IC95%:1,03;1,17) e os insatisfeitos com o trabalho (RP=1,21; IC95%:1,15;1,27). Como fator de proteção encontraram-se os professores com mais tempo de trabalho docente (RP=0,92; IC95%:0,87;0,98) e contratados ou designados (RP=0,89; IC95%:0,85;0,94). Conclusão: Verificou-se inadequação do estilo de vida entre os professores homens mais velhos, com maior jornada laboral e insatisfação com o trabalho.

Abstract This study describes a methodology to prepare and characterize PUF [poly(urea formaldehyde)] microcapsules containing 5-ethylidene-2-norbornene (ENB) as self-healing agent, as well as the compatibility evaluation of ENB in the presence of Grubb’s catalyst with an epoxy matrix. First, the results of an adhesion assay by lap shear proved chemical compatibility between the epoxy matrix and self-healing agent in the presence of Grubb’s catalyst. After evaluating the chemical compatibility, microcapsules with ENB were synthesized in an oil-in-water emulsion system. Thereafter, the microcapsules were morphologically, chemically and thermally characterized in which a granulometric dispersion between 30-140 μm and an average size of 69.8 ± 4.9 μm were observed. The SEM (scanning electron microscope) results showed that the average thickness of microcapsules shell was 3.6 ± 0.4 μm. The thermogravimetric analysis (TGA) showed that microcapsules are susceptible to rupture and consequent ENB release in temperatures greater than 230 °C, demonstrating that microcapsules are suitable for applications in materials with self-healing capacity.

Abstract The objective of this work was to determine the effect of planting densities on the yield and quality of 'Hass' avocado (Persea americana) in the department of Antioquia, Colombia. The experimental design was randomized complete blocks with three replicates. The treatments were six plant densities (204, 278, 333, 400, 625, and 816 trees per hectare) with five harvest seasons, and each experimental unit consisted of six nine-year-old trees. The highest fruit yield is obtained at 333 and 400 trees per hectare. The main harvest represents 70% (18 Mg ha-1) of the annual production, whereas the secondary (mitaca) harvest represents 30% (5.25 Mg ha-1). Yield per tree and number of avocado fruits per tree are negatively affected by the increase in planting densities. In addition, fruit quality parameters show better results at intermediate planting densities of 333 and 400 trees per hectare, with the highest ratios of mesocarp and the lowest of seed, both in fresh and dry weight.

Resumo O objetivo deste trabalho foi determinar o efeito de densidades de plantio na produção e na qualidade do abacateiro 'Hass' (Persea americana) no departamento de Antioquia, Colômbia. O delineamento experimental foi de blocos ao acaso, com três repetições. Os tratamentos foram seis densidades de plantio (204, 278, 333, 400, 625 e 816 árvores por hectare) com cinco colheitas, e cada unidade experimental consistiu em seis árvores com nove anos de idade. O maior rendimento é obtido às densidades de 333 e 400 árvores por hectare. A colheita principal representa 70% (18 Mg ha-1) da produção anual, enquanto a colheita secundária (mitaca) representa 30% (5.25 Mg ha-1). A produção por árvore e o número de frutos de abacate por árvore é afetado negativamente pelo aumento das densidades de plantio. Além disso, os parâmetros de qualidade dos frutos apresentam melhores resultados nas densidades de plantio intermediárias de 333 e 400 árvores por hectare, com as maiores proporções de mesocarpo e a menor de sementes, tanto em massa fresca quanto em seca.

RESUMO As alterações morfológicas no porta-enxerto/copa são uma das limitações no uso da enxertia, em frutíferas, sendo definida como uma incompatibilidade entre esses dois tecidos. No entatnto os efeitos das interações porta-enxerto/copa no potencial reprodutivo, frutificação, eficiência de produção e características de qualidade do abacate são complexos e pouco compreendidos. Sendo o presente trabalho teve como objetivo avaliar o crescimento do fruto do abacateiro cv. Hass em árvores com incompatibilidades entre o porta-enxerto e o enxerto nas principais regiões produtoras da Colômbia. Foi utilizado um delineamento de parcelas subdivididas com fator de bloqueio de localidade. A parcela principal correspondeu à compatibilidade e fator de colheita e as sub parcelas à idade de desenvolvimento dos frutos. A análise estatística consistiu um modelo linear misto para as variáveis frequência respiratória e crescimento morfológico do fruto, realizando-se um teste de diferenças múltiplas significativas utilizando o ajuste para multiplicidade por família através da correção de Holm. O tratamento de compatibilidade e a época de colheita não afetaram a taxa respiratória dos frutos nem as variáveis de crescimento e desenvolvimento da cv. Hass. No entanto, a idade de desenvolvimento do fruto afetou significativamente tanto a taxa respiratória quanto as variáveis de crescimento dos frutos. portaenxerto/copa portaenxertocopa porta enxerto/copa copa enxertia frutíferas tecidos reprodutivo frutificação compreendidos cv portaenxerto Colômbia localidade realizandose realizando se Holm entanto enxertocopa

ABSTRACT The rootstock/scion morphological alterations are one of the limitations in the use of grafting, which has been defined as an incompatibility between these two tissues. However the effect of rootstock-scion interactions on reproductive potential, fruit set, yield efficiency, and avocado fruit quality characteristics are complex and poorly understood. This research aimed to evaluate the fruit growth of avocado cv. Hass in trees with incompatibilities between the rootstock and the graft in the main producing regions in Colombia. The split-plot design with a locality-blocking factor was used. The main plot corresponded to the compatibility and harvest factor, and the subplots to the age of fruit development. The statistical analysis consisted of a mixed linear model for the variables of respiratory rate and morphological growth of the fruit, performing a significant multiple difference test using the adjustment for multiplicity by family through Holm’s correction. The compatibility treatment and the harvest season did not affect the fruit respiratory rate nor the variables of growth and development of cv. Hass. However, the age of development significantly affected both the respiratory rate and the variables of fruit growth. rootstockscion scion grafting tissues potential set efficiency understood cv Colombia splitplot split localityblocking locality blocking used Holms Holm s correction