Search | SciELO

Need help?

Add field +

Search history

No documents were found for your search

Results: 19

au:Lopes, Ana Catarina Souza

Filters

Collection + Options

All
Brazil

18
Rve

1

Journal + Options

All
Revista da Sociedade Brasileira de Medicina Tropical

11
Revista da Escola de Enfermagem da USP

2
Brazilian Journal of Infectious Diseases

1
Ciência Florestal

1
Revista Brasileira de Saúde Materno Infantil

1
Revista Brasileira de Terapia Intensiva

1
Revista do Instituto de Medicina Tropical de São Paulo

1
Einstein (São Paulo)

1

Language

All
Portuguese

6

Publication Year + Options

All
2012

4
2019

3
2020

3
2015

2
2003

1
2009

1
2013

1
2014

1
2016

1
2017

1
2021

1

SciELO Thematic Areas + Options

All
Health Sciences

18
Biological Sciences

2
Agricultural Sciences

1

WoS Subject Categories + Options

All
Medicine

14
Tropical

12
Care

1
Critical

1
Diseases

1
Ecology

1
Environmental

1
Forestry

1
General

1
Infectious

1
Internal

1
Nursing

1
Pediatrics

1
Studies

1

WoS Citation Index + Options

All
Science Citation Index Expanded

15
Social Sciences Citation Index

2

Citables and non citables

All
Citable

19

Type of Literature + Options

All
Article

12
Rapid communication

6
Case report

1

Order by

Page of 2 Next

Select this page

Print | Send to email | Export |

Facebook
Twitter
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

0 Selected itens

List all
Erase all

First report of bla GES-1 in Proteus mirabilis clinical isolates
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Beltrão, Elizabeth Maria Bispo

; Oliveira, Érica Maria de

; Lopes, Ana Catarina de Souza

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2021, Volume 54 elocation e0864-2020

Abstract: En | Text: En | PDF: En

Abstract Proteus mirabilis is one of the main pathogens causing urinary tract infections and sepsis. To our knowledge, this is the first report of a P. mirabilis hosting bla GES. The presence of these genes was determined using PCR and sequencing. We identified the presence of bla GES-1 in all three isolates. In addition, we identified the bla KPC-2 and bla NDM-1 genes in the two strains. These data emphasize the importance of monitoring and surveillance of all enterobacteria. The circulation of P. mirabilis strains carrying bla GES-1 constitutes a new scenario of resistance in this species and should be an epidemiological alert for global health.

https://doi.org/10.1590/0037-8682-0864-2020 223 downloads

High plasmid variability, and the presence of IncFIB, IncQ, IncA/C, IncHI1B, and IncL/M in clinical isolates of Klebsiella pneumoniae with bla KPC and bla NDM from patients at a public hospital in Brazil.
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Oliveira, Érica Maria de

; Beltrão, Elizabeth Maria Bispo

; Scavuzzi, Alexsandra Maria Lima

; Barros, Josineide Ferreira

; Lopes, Ana Catarina Souza

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2020, Volume 53 elocation e20200397

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION Antibiotic resistance in carbapenemase-producing Klebsiella pneumoniae is acquired and disseminated mainly by plasmids. Therefore, we aimed to investigate the occurrence of carbapenemase genes, analyze the genetic diversity by ERIC-PCR, and examine the most common plasmid incompatibility groups (Incs) in clinical isolates of K. pneumoniae from colonization and infection in patients from a hospital in Brazil. METHODS Twenty-seven isolates of carbapenem-resistant K. pneumoniae were selected and screened for the presence of carbapenemase genes and Incs by PCR, followed by amplicon sequencing. RESULTS The bla KPC and bla NDM genes were detected in 24 (88.8 %) and 16 (59.2 %) of the isolates, respectively. Thirteen isolates (48.1 %) were positive for both genes. The IncFIB (92.6 %) and IncQ (88.8 %) were the most frequent plasmids, followed by IncA/C, IncHI1B, and IncL/M, indicating that plasmid variability existed in these isolates. To our knowledge, this is the first report of IncHI1B in Brazil. We found eight isolates with clonal relationship distributed in different sectors of the hospital. CONCLUSIONS The accumulation of resistance determinants, the variability of plasmid Incs, and the clonal dissemination detected in K. pneumoniae isolates demonstrate their potential for infection, colonization, and the dissemination of different resistance genes and plasmids.

https://doi.org/10.1590/0037-8682-0397-2020 486 downloads

Brazilian Nutritional Consensus in Hematopoietic Stem Cell Transplantation: Graft- versus -host disease
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Pereira, Andréa Z

; Vigorito, Afonso Celso

; Almeida, Alessandro de Moura

; Candolo, Alexandre de Almeida

; Silva, Ana Carolina Leão

; Brandão-Anjos, Ana Elisa de Paula

; Sá, Bianca Laselva de

; Souza, Catarina Lôbo Santos de

; Castro Junior, Cláudio Galvão de

; Oliveira, José Salvador Rodrigues de

; Barban, Juliana Bernardo

; Mancilha, Elaine Maria Borges

; Todaro, Juliana

; Lopes, Lilian Pinheiro

; Macedo, Maria Cristina Martins de Almeida

; Rodrigues, Morgani

; Ribeiro, Paulo Cesar

; Silva, Roberto Luiz da

; Roberto, Telma Sigolo

; Rodrigues, Thays de Cássia Ruiz

; Colturato, Vergilio Antonio Rensi

; Paton, Eduardo José de Alencar

; Barros, George Maurício Navarro

; Almeida, Rosana Ducatti Souza

; Moreira, Maria Claudia Rodrigues

; Flowers, Mary Evelyn

einstein (São Paulo)

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2020, Volume 18 elocation eAE4799

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O Consenso Brasileiro de Nutrição no Transplante de Células Tronco Hematopoiéticas: doença do enxerto contra o hospedeiro foi aprovado pela Sociedade Brasileira de Transplante de Medula Óssea, com a participação de 26 centros brasileiros de transplante de células-tronco hematopoiéticas. O Consenso descreve as principais condutas nutricionais em casos de doença do enxerto contra o hospedeiro, a principal complicação do transplante de células-tronco hematopoiéticas.

ABSTRACT The Brazilian Consensus on Nutrition in Hematopoietic Stem Cell Transplantation: Graft- versus -host disease was approved by Sociedade Brasileira de Transplante de Medula Óssea , with the participation of 26 Brazilian hematopoietic stem cell transplantation centers. It describes the main nutritional protocols in cases of Graft- versus -host disease, the main complication of hematopoietic stem cell transplantation.

https://doi.org/10.31744/einstein_journal/2020ae4799 1552 downloads

Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Lima, Giselle Jucá de

; Scavuzzi, Alexsandra Maria Lima

; Beltrão, Elizabeth Maria Bispo

; Firmo, Elza Ferreira

; Oliveira, Érica Maria de

; Oliveira, Sibele Ribeiro de

; Rezende, Antônio Mauro

; Lopes, Ana Catarina de Souza

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2020, Volume 53 elocation e20190526

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION: This study investigated the genetic environment of bla KPC-2 in Klebsiella pnemoniae multi-drug resistant clinical isolates. METHODS: Four carbapenemase gene isolates resistant to carbapenems, collected from infected patients from two hospitals in Brazil, were investigated using polymerase chain reaction and plasmid DNA sequencing. RESULTS: The bla KPC-2 gene was located between ISKpn6 and a resolvase tnpR in the non-Tn4401 element (NTEKPC-IId). It was detected on a plasmid belonging to the IncQ1 group. CONCLUSIONS To our knowledge, this is the first report of the presence of the bla KPC-2 gene in the NTEKPC-IId element carried by plasmid IncQ1 from infections in Brazil.

https://doi.org/10.1590/0037-8682-0526-2019 504 downloads

Emergence of bla NDM-1 associated with the aac(6’)-Ib-cr, acrB, cps, and mrkD genes in a clinical isolate of multi-drug resistant Klebsiella pneumoniae from Recife-PE, Brazil
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Scavuzzi, Alexsandra Maria Lima

; Firmo, Elza Ferreira

; Oliveira, Érica Maria de

; Lopes, Ana Catarina de Souza

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2019, Volume 52 elocation e20180352

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION: The emergence of New Delhi metallo-β-lactamase (NDM) is concernig because it reduces the antibiotic therapy options for bacterial infections. METHODS: Resistant and virulent genes from an isolate of Klebsiella pneumoniae derived from a patient with sepsis in a hospital in Recife-PE, Brazil, were investigated using PCR and DNA sequencing. RESULTS: bla NDM-1, aac(6’)-Ib-cr and acrB resistance genes, and cps and mrkD virulence genes were detected. CONCLUSIONS To our knowledge, this is the first report on bla NDM-1 in Recife-PE. This detection alerts researchers to the need to control the spread of bla NDM-1 resistance gene by this bacterium in Brazil.

https://doi.org/10.1590/0037-8682-0352-2018 1678 downloads

Relationships between phagocytosis, mucoid phenotype, and genetic characteristics of Klebsiella pneumoniae clinical isolates
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Cavalcanti, Francinete Carla Nunes

; Rodrigues, Juliana Falcão

; Cabral, Adriane Borges

; Azarias, Larissa Caina Barbosa de

; Morais Júnior, Marcos Antônio de

; Castro, Célia Maria Machado Barbosa de

; Lopes, Ana Catarina de Souza

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2019, Volume 52 elocation e20190089B

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION The relationships between phagocytosis, and mucoid phenotype, plasmid profile and virulence, and resistance genetic characteristics of Klebsiella pneumoniae clinical isolates were evaluated. METHODS Thirty isolates were used to determine the mucoid aspect. Four were selected for analysis of phagocytosis by alveolar macrophages. RESULTS Thirty percent of the samples presented the mucoid phenotype. The phagocytosis rate ranged from 21.5% to 43.43%. Phagocytosis was not correlated with the plasmid profile, but was apparently correlated with mucoid phenotype and antibiotic susceptibility. CONCLUSIONS: Several virulence factors act in parallel in K. pneumoniae to impair host defense.

https://doi.org/10.1590/0037-8682-0089-2019 1024 downloads

Emergence of aph(3’)-VI and accumulation of aminoglycoside modifying enzyme genes in KPC-2-possessing Enterobacter aerogenes isolates from infections and colonization in patients from Recife-PE, Brazil
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Firmo, Elza Ferreira

; Cabral, Adriane Borges

; Oliveira, Érica Maria de

; Lopes, Ana Catarina Souza

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

2019, Volume 52 elocation e20180460

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION: The objective of this study was to characterize genes of aminoglycoside modifying enzymes (AMEs) in colonizing and infecting isolates of E. aerogenes harboring bla KPC from patients at a public hospital in Recife-PE, Brazil. METHODS: We analyzed 29 E. aerogenes clinical isolates resistant to aminoglycosides. AMEs genes were investigated by PCR and sequencing. RESULTS: Colonizing and infecting isolates mainly presented the genetic profiles aac(3)-IIa/aph(3’)-VI or ant(2”)-IIa/aph(3’)-VI. This is the first report of aph(3’)-VI in E. aerogenes harboring bla KPC in Brazil. CONCLUSIONS: The results highlight the importance in establishing rigorous methods for the surveillance of resistance genes, especially in colonized patients.

https://doi.org/10.1590/0037-8682-0460-2018 802 downloads

Detection of multidrug-resistant Pseudomonas aeruginosa harboring bla GES-1 and bla GES-11 in Recife, Brazil
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Silva Júnior, Valdemir Vicente da

; Ferreira, Laura Durão

; Alves, Lílian Rodrigues

; Cabral, Adriane Borges

; Jácome, Paula Regina Luna de Araújo

; Araújo, Paulo Sérgio Ramos de

; Lopes, Ana Catarina de Souza

; Maciel, Maria Amélia Vieira

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

dec 2017, Volume 50 N. 6 Pages 764 - 768

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION: Pseudomonas aeruginosa, an important pathogen globally, presents several resistance mechanisms. This study aimed to investigate the presence of bla GES in clinical isolates of Pseudomonas aeruginosa obtained from various clinical specimens from patients admitted to three different hospitals in Recife, Brazil. The Guiana extended spectrum beta-lactamase (GES) enzymes are responsible for conferring broad spectrum resistance to beta-lactam drugs, including the carbapenems. METHODS: A total of 100 carbapenem-resistant P. aeruginosa isolates underwent polymerase chain reaction (PCR) testing to identify bla GES, bla KPC, bla SPM-1, bla IMP, and bla VIM. Additionally, PCR products positive for bla GES were sequenced. The clonal profiles of these same isolates were then determined by means of enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis. RESULTS: PCR analysis revealed that four isolates harbored bla GES; DNA sequencing showed that two harbored bla GES-1 and two bla GES-11. Beta-lactamase genes bla SPM-1, bla IMP, bla VIM, and bla KPC were investigated; none of these genes was detected. Automated susceptibility testing methods (Vitek®2, bioMérieux) showed that the bla GES-1-positive isolates were only susceptible to polymyxin B. The patterns obtained with ERIC-PCR methods showed clonal relationship between the two isolates that harbored bla GES-11, whereas different clonal profiles were found in the isolates harboring bla GES-1. CONCLUSIONS: We detected the presence of bacterial isolates positive for two different variants of the enzyme GES in three different hospitals from Recife, Brazil. These enzymes have a great capacity for dissemination among Gram-negative bacteria and confer broad-spectrum resistance to beta-lactam antibiotics and to the carbapenems.

https://doi.org/10.1590/0037-8682-0532-2016 1887 downloads

Phenotypic and molecular characterization of resistance to macrolides, lincosamides and type B streptogramin of clinical isolates of Staphylococcus spp. of a university hospital in Recife, Pernambuco, Brazil
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Pereira, Jussyêgles Niedja da Paz

; Rabelo, Marcelle Aquino

; Lima, Jailton Lobo da Costa

; Neto, Armando Monteiro Bezerra

; Lopes, Ana Catarina de Souza

; Maciel, Maria Amélia Vieira

Brazilian Journal of Infectious Diseases

Journal Metrics
- Sobre o periódico
- SciELO Analytics

jun 2016, Volume 20 N. 3 Pages 276 - 281

Abstract: En | Text: En | PDF: En

Abstract Introduction There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail. Objective To characterize the phenotypic (occurrence of cMLSB and iMLSB phenotypes) and molecular (occurrence of ermA and ermC genes) profiles of MLSB resistance of clinical isolates of susceptible and methicillin-resistant Staphylococcus aureus and CNS (coagulase-negative Staphylococcus) from patients of a university hospital, in Pernambuco. Methods The antimicrobial susceptibility of 103 isolates was determined by the disk diffusion technique in Mueller–Hinton agar followed by oxacillin screening. The iMLSB phenotype was detected by D test. Isolates with cMLSB and iMLSB phenotypes were subjected to polymerase chain reaction (PCR) for the detection of ermA and ermC genes. Results The cMLSB and iMLSB phenotypes were respectively identified in 39 (37.9%) and five (4.9%) isolates. The iMLSB phenotype was found only in four (10.8%) methicillin-susceptible S. aureus and one (4.5%) methicillin-resistant S. aureus. In the 44 isolates subjected to PCR, four (9.1%) only ermA gene was detected, a lower frequency when compared to only ermC 17 (38.6%) gene and to one (2.3%) isolate presenting both genes. Conclusion In the Staphylococcus spp. analyzed, the ermC gene was found more often than the ermA, although the iMLSB phenotype had been less frequent than the cMLSB. It was important to perform the D test for its detection to guide therapeutic approaches.

https://doi.org/10.1016/j.bjid.2016.03.003 940 downloads

10.

KPC-PRODUCING Serratia marcescens IN A HOME-CARE PATIENT FROM RECIFE, BRAZIL
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

MARGATE, Emmily

; MAGALHÃES, Vera

; FEHLBERG, Lorena Cristina Corrêa

; GALES, Ana Cristina

; LOPES, Ana Catarina Souza

Revista do Instituto de Medicina Tropical de São Paulo

Journal Metrics
- Sobre o periódico
- SciELO Analytics

aug 2015, Volume 57 N. 4 Pages 359 - 360

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Nesse estudo descrevemos a ocorrência de um isolado de Serratia marcescensprodutor de KPC em um paciente sob assistência médica domiciliar em Recife, Brazil. Os genes blaKPC, blaSPM, blaIMP, blaVIMblaOXA, blaCTX-M, blaSHV, blaTEM and blaGES foram investigados pela Reação em Cadeia da Polimerase (PCR) e sequenciamento de DNA. O isolado foi positivo para os genes blaKPC-2 and blaTEM-1 e foi resistente a aztreonam, cefepime, cefotaxima, imipenem, meropenem, gentamicina, ciprofloxacina e ceftazidima, e susceptível apenas a amicacina, tigeciclina e gatifloxacina. Este é o primeiro relato no Brasil de um isolado clínico de S. marcescensprodutor de KPC fora de ambiente hospitalar. Os profissionais de saúde devem estar atentos à presença desse isolado na comunidade.

SUMMARY In this brief communication we describe the occurrence of a KPC-producing Serratia marcescensisolate in a home-care patient from Recife, Brazil. The blaKPC, blaSPM, blaIMP, blaVIMblaOXA, blaCTX-M, blaSHV, blaTEM and blaGES genes were investigated by Polymerase Chain Reaction (PCR) and DNA sequencing. The isolate was positive for blaKPC-2 and blaTEM-1 and was resistant to aztreonam, cefepime, cefotaxime, imipenem, meropenem, gentamicin, ciprofloxacin and cefazidime, and susceptible only to amikacin, tigecycline and gatifloxacin. This is the first report in Brazil of KPC-producing S. marcescens clinical isolate outside of a hospital environment. Caregivers should be alert for the presence of this isolate in the community setting.

https://doi.org/10.1590/S0036-46652015000400016 1994 downloads

11.

Detection of bla KPC-2 in Proteus mirabilis in Brazil
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Cabral, Adriane Borges

; Maciel, Maria Amélia Vieira

; Barros, Josineide Ferreira

; Antunes, Marcelo Maranhão

; Lopes, Ana Catarina Souza

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

feb 2015, Volume 48 N. 1 Pages 94 - 95

Abstract: En | Text: En | PDF: En

INTRODUCTION : Infections caused by Klebsiella pneumoniae carbapenemase (KPC)-producing isolates pose a major worldwide public health problem today. METHODS : A carbapenem-resistant Proteus mirabilis clinical isolate was investigated for plasmid profiles and the occurrence of β-lactamase genes. RESULTS : The isolate exhibited resistance to ertapenem and imipenem and was susceptible to meropenem, polymyxin, and tigecycline. Five plasmids were identified in this isolate. DNA sequencing analysis revealed the presence of bla KPC-2 and bla TEM-1 genes. An additional PCR using plasmid DNA confirmed that bla KPC-2 was present in one of these plasmids. Conclusions: We report the detection of bla KPC-2 in P. mirabilis in Brazil for the first time. This finding highlights the continuous transfer of bla KPC between bacterial genera, which presents a serious challenge to the prevention of infection by multidrug-resistant bacteria.

https://doi.org/10.1590/0037-8682-0152-2014 2703 downloads

12.

Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Lima, Alexsandra Maria Silva

; Melo, Maíra Espíndola Silva de

; Alves, Luiz Carlos

; Brayner, Fábio André

; Lopes, Ana Catarina Souza

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

apr 2014, Volume 47 N. 2 Pages 165 - 169

Abstract: En | Text: En | PDF: En

Introduction The high prevalence of Klebsiella pneumoniae infections is related to the ability of K. pneumoniae to acquire and disseminate exogenous genes associated with mobile elements, such as R plasmids, transposons and integrons. This study investigated the presence of class 1 integrons in clinical and microbiota isolates of K. pneumoniae belonging to different phylogenetic groups and correlated these results with the antimicrobial resistance profiles of the studied isolates. Methods Of the 51 isolates of K. pneumoniae selected for this study, 29 were from multidrug-resistant clinical isolates, and 22 were from children's microbiota. The susceptibility profile was determined using the disk diffusion method, and class 1 integrons were detected through polymerase chain reaction (PCR). Results The results showed that none of the 22 microbiota isolates carried class 1 integrons. Among the 29 clinical isolates, 19 (65.5%) contained class 1 integrons, and resistance to sulfamethoxazole/trimethoprim was identified in 18 of these isolates (94.7%). Among the K. pneumoniae isolates with class 1 integrons, 47% belonged to the KpI phylogenetic group, and one isolate (14.3%) carrying these genetic elements belonged to the KpIII group. Conclusions The wide variety of detected class 1 integrons supports the presence of high rates of antimicrobial resistance, genetic variability, and rapid dissemination of beta-lactamase genes among K. pneumoniae clinical isolates in recent years in hospitals in Recife-PE, Brazil. The findings of this study indicate that the surveillance of K. pneumoniae integrons in clinical isolates could be useful for monitoring the spread of antibiotic resistance genes in the hospital environment.

https://doi.org/10.1590/0037-8682-0021-2014 2993 downloads

13.

FLORÍSTICA E ESTRUTURA DO COMPONENTE ARBÓREO E ANÁLISE AMBIENTAL DE UM FRAGMENTO DE FLORESTA OMBRÓFILA MISTA ALTO-MONTANA NO MUNICÍPIO DE PAINEL, SC
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics
- Dimensions
- PlumX
- Altmetric

Higuchi, Pedro

; Silva, Ana Carolina da

; Almeida, Jaime Antonio de

; Bortoluzzi, Roseli Lopes da Costa

; Mantovani, Adelar

; Ferreira, Tiago de Souza

; Souza, Sheila Trierveiler de

; Gomes, Juliano Pereira

; Silva, Karina Montibeller da

Ciência Florestal

Journal Metrics
- Sobre o periódico
- SciELO Analytics

mar 2013, Volume 23 N. 1 Pages 153 - 164

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A Floresta Ombrófila Mista Alto-Montana é uma formação pouco estudada que ocorre em altitudes acima de 1.000 m. Os objetivos deste estudo foram conhecer os padrões florísticos e estruturais do componente arbóreo de um fragmento desta floresta na região do Planalto Sul Catarinense e determinar as variáveis ambientais que influenciam esses padrões. O levantamento da composição florística e estrutural e a coleta das variáveis ambientais foram conduzidos em 50 parcelas de 200 m2. Nelas, todos os indivíduos arbóreos com CAP (circunferência medida a altura do peito) ≥ 15,7 cm foram medidos (CAP e altura) e identificados. Foram coletadas, em cada parcela, variáveis ambientais relacionadas às características químicas e físicas dos solos, topográficas e de cobertura do dossel. Foram calculados os parâmetros fitossociológicos e a estrutura diamétrica da comunidade e das populações com valor de importância (VI) acima de 5 %. A similaridade florístico-estrutural entre as parcelas foi analisada pela NMDS (Nonmetric Multidimensional Scaling) e os vetores das variáveis ambientais significativas (p < 0,05) foram plotados a posteriori. Foram identificadas 50 espécies arbóreas distribuídas em 33 gêneros e 20 famílias botânicas. As espécies com maior VI foram: Araucaria angustifolia (Bertol.) Kuntze (17,32 %), Myrceugenia euosma (O.Berg) D.Legrand (15,24 %) e Acca sellowiana (O.Berg) Burret (7,84 %). A estrutura diamétrica de toda a comunidade e das populações estudadas (exceto Dicksonia sellowiana Hook.) teve distribuição próxima ao "J invertido". A análise NMDS demonstrou maior porcentagem de argila nas parcelas com maior densidade de Araucaria angustifolia e menor porcentagem, nas parcelas com maior densidade de Dicksonia sellowiana, Inga lentiscifolia Benth. e Ocotea pulchella Mart. As parcelas de maior declividade tiveram maior densidade de Drimys brasiliensis Miers e aquelas de menor declividade, maior cota e maior cobertura do dossel, tiveram maior ocorrência de Drimys angustifolia Miers, Prunus myrtifolia (L.) Urb., Calyptranthes concinna DC. e Myrceugenia oxysepala (Burret) D.Legrand & Kausel.

ABSTRACT The highland Araucaria Forest is a little-studied forest formation, occurring in altitudes above 1,000 m. The objectives of this study were to understand the structural and floristic patterns of the tree component of a remaining of this forest in the southern plateau region of Santa Catarina State and to determine the environmental variables that influence these patterns. The tree component survey and the environmental data collection were conducted in 50 plots of 200 m2. Within these plots, all living trees with circumference at breast height (CBH) ≥ 15,7 cm were measured (CBH and height) and identified. In each plot, environmental variables related to soils physical and chemical traits, topography and canopy cover were collected. Phytosociological parameters and the diameter structure (whole tree community and tree populations with the importance value above 5 %) were calculated. The floristic-structural similarities among plots were analyzed by NMDS (Nonmetric Multidimensional Scaling) and vectors of environmental variables (p < 0.05) were plotted a posteriori. A total of 50 tree species were identified, distributed in 33 genera and 20 families. The species with the highest VI were Araucaria angustifolia (Bertol.) Kuntze (17.32 %), Myrceugenia euosma (O. Berg) D. Legrand (15.24 %) and Acca sellowiana (O. Berg) Burret (7.84 % ). The diameter structure of the whole community and of the study populations (except Dicksonia sellowiana Hook.) showed a distribution close to the "inverted J". The NMDS analysis showed a higher percentage of clay in the plots with the highest density of Acca sellowiana and lowest percentage in the plots with high density of Dicksonia sellowiana, Inga lentiscifolia Benth. and Ocotea pulchella Mart. Plots with higher declivity had a higher density of Drimys brasiliensis Miers and those of lower declivity, higher elevation and greater canopy closure, had a higher occurrence of Drimys angustifolia Miers, Prunus myrtifolia (L.) Urb, Calyptranthes concinna DC. and Myrceugenia oxysepala (Burret) D. Legrand & Kausel.

https://doi.org/10.5902/198050988449 1027 downloads

14.

Phenotypic and molecular characterization of antimicrobial resistance and virulence factors in Pseudomonas aeruginosa clinical isolates from Recife, State of Pernambuco, Brazil
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics

Jácome, Paula Regina Luna de Araújo

; Alves, Lílian Rodrigues

; Cabral, Adriane Borges

; Lopes, Ana Catarina Souza

; Maciel, Maria Amélia Vieira

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

dec 2012, Volume 45 N. 6 Pages 707 - 712

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

INTRODUÇÃO: A emergência de mecanismos de resistência aos carbapenêmicos em Pseudomonas aeruginosa tem se destacado devido ao amplo espectro de degradação de antimicrobianos, reduzindo as opções terapêuticas. MÉTODOS: Sessenta e um isolados de P. aeruginosa procedentes de cinco hospitais públicos de Recife, Pernambuco, Brasil, entre 2006 e 2010, foram analisadas, com o objetivo de avaliar o perfil de virulência, resistência aos antimicrobianos, a presença de genes metalo-β-lactamase (MBL) e a relação clonal entre os isolados. RESULTADOS: Foi observada uma elevada produção de fatores de virulência na amostra (34,4% colônias mucoides; 70,5% piocianina; 93,4% gelatinase e 72,1% hemolisina), bem como um elevado percentual de resistência (4,9% isolados panresistentes e 54,1% multirresistentes). Dentre os 29 isolados resistentes ao imipenem e/ou ceftazidima, 44,8% (13/29) apresentaram MBL por meio da pesquisa fenotípica, e destes, 46,2% (6/13) foram positivos para o gene blaSPM-1, não havendo detecção dos genes blaIMP e blaVIM. A tipagem molecular revelou 21 perfis genéticos dos quais sete foram detectados em hospitais e períodos distintos, e dos isolados blaSPM-1 positivos, três apresentaram o mesmo perfil clonal e foram procedentes do mesmo hospital, enquanto que os outros três isolados blaSPM-1 positivos apresentaram perfis clonais distintos. CONCLUSÕES: Estes resultados revelam que a P. aeruginosa é capaz de acumular diferentes fatores de virulência e resistência, dificultando o tratamento das infecções. A identificação de genes blaSPM-1 e disseminação de clones sugere a necessidade de aplicação mais rigorosa de medidas de controle de infecção nos hospitais de Recife, visando reduzir custos e danos provocados por este tipo de infecção.

INTRODUCTION: The emergence of carbapenem resistance mechanisms in Pseudomonas aeruginosa has been outstanding due to the wide spectrum of antimicrobial degradation of these bacteria, reducing of therapeutic options. METHODS: Sixty-one clinical strains of P. aeruginosa isolated from five public hospitals in Recife, Pernambuco, Brazil, were examined between 2006 and 2010, aiming of evaluating the profiles of virulence, resistance to antimicrobials, presence of metallo-β-lactamase (MBL) genes, and clonal relationship among isolates. RESULTS: A high percentage of virulence factors (34.4% mucoid colonies; 70.5% pyocyanin; 93.4% gelatinase positives; and 72.1% hemolysin positive) and a high percentage of antimicrobial resistance rates (4.9% pan-resistant and 54.1% multi-drug resistant isolates) were observed. Among the 29 isolates resistant to imipenem and/or ceftazidime, 44.8% (13/29) were MBL producers by phenotypic evaluation, and of these, 46.2% (6/13) were positive for the blaSPM-1 gene. The blaIMP and blaVIM genes were not detected. The molecular typing revealed 21 molecular profiles of which seven were detected in distinct hospitals and periods. Among the six positive blaSPM-1 isolates, three presented the same clonal profile and were from the same hospital, whereas the other three presented different clonal profiles. CONCLUSIONS: These results revealed that P. aeruginosa is able to accumulate different resistance and virulence factors, making the treatment of infections difficult. The identification of blaSPM-1 genes and the dissemination of clones in different hospitals, indicate the need for stricter application of infection control measures in hospitals in Recife, Brazil, aiming at reducing costs and damages caused by P. aeruginosa infections.

10208 downloads

15.

Multidrug resistance genes, including blaKPC and blaCTX-M-2, among Klebsiella pneumoniae isolated in Recife, Brazil
Facebook Twitter

Other social networks
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley
Other networks

Metrics
- SciELO Analytics

Cabral, Adriane Borges

; Melo, Rita de Cássia de Andrade

; Maciel, Maria Amélia Vieira

; Lopes, Ana Catarina Souza

Revista da Sociedade Brasileira de Medicina Tropical

Journal Metrics
- Sobre o periódico
- SciELO Analytics

oct 2012, Volume 45 N. 5 Pages 572 - 578

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

INTRODUÇÃO: A prevalência de cepas de Klebsiella pneumoniae resistentes a cefalosporinas e carbapenêmicos está aumentando no Brasil, com sérias consequências em termos de desfechos dos pacientes e cuidados gerais. MÉTODOS: Este estudo caracterizou 24 isolados clínicos de K. pneumoniae provenientes de dois hospitais de Recife, Brasil, através do perfil de susceptibilidade a antimicrobianos, análise de genes de β-lactamase (blaTEM,blaSHV,blaCTX-MblaKPC,blaVIM, blaIMP,and blaSPM), perfil plasmidial e ERIC-PCR (Enterobacterial repetitive intergenic consensus-polymerase chain reaction). RESULTADOS: A análise da ERIC-PCR e do perfil plasmidial agrupou os isolados em 17 e 19 perfis, respectivamente. Seis isolados de um hospital apresentaram o mesmo padrão de ERIC-PCR, indicando disseminação clonal. Todos os isolados apresentaram blaSHV, 62,5% apresentaram blaCTX-M-2, 29% blaTEM e 41,7% blaKPC. Genes de metalo-β-lactamase blaVIM, blaIMP e blaSPM não foram detectados. Onze isolados foram identificados carreando, pelo menos, três dos genes de β-lactamase estudados, dentre estes, dois isolados continham blaSHV,blaTEM, blaCTX-M-2 e blaKPC simultaneamente. CONCLUSÕES: O acúmulo de genes de resistência em algumas cepas, observado nesse estudo, impõem limitações nas opções terapêuticas disponíveis para o tratamento de infecções causadas por K. pneumoniae em Recife, Brasil. Estes resultados devem alertar as autoridades médicas brasileiras para estabelecer rigorosos métodos para controlar eficientemente a disseminação de genes de resistência a antimicrobianos no ambiente hospitalar.

INTRODUCTION: The prevalence of cephalosporins and carbapenem-resistant Klebsiella pneumoniae strains is rising in Brazil, with potential serious consequences in terms of patients' outcomes and general care. METHODS: This study characterized 24 clinical isolates of K. pneumoniae from two hospitals in Recife, Brazil, through the antimicrobial susceptibility profile, analyses of β-lactamase genes (blaTEM, blaSHV,blaCTX-MblaKPC, blaVIM, blaIMP, and blaSPM), plasmidial profile and ERIC-PCR (Enterobacterial repetitive intergenic consensus-polymerase chain reaction). RESULTS: ERIC-PCR and plasmidial analysis grouped the isolates in 17 and 19 patterns, respectively. Six isolates from one hospital presented the same pattern by ERIC-PCR, indicating clonal dissemination. All isolates presented blaSHV, 62.5% presented blaCTX-M-2, 29% blaTEM, and 41.7% blaKPC. Metallo-β-lactamase genes blaand blawere not detected. Eleven isolates were identified carrying at least 3 β-lactamase studied genes, and 2 isolates carried blaSHVblaTEM, blaCTX-M-2 and blaKPC simultaneously. CONCLUSIONS: The accumulation of resistance genes in some strains, observed in this study, imposes limitations in the therapeutic options available for the treatment of infections caused by K. pneumoniae in Recife, Brazil. These results should alert the Brazilian medical authorities to establish rigorous methods for more efficiently control the dissemination of antimicrobial resistance genes in the hospital environment.

10760 downloads Cited 7 times in SciELO

Showing

itens per page

Page of 2 Next

Select one or more results

All Alphabetic

Results

Comparative chart Download CSV

Statistics of

Send result

To*

Use ; (semicolon) to insert more emails.

Send to email

This page

Selected records

All references (max. 300)

Your email

Subject

Comments

Export format:

RIS (for EndNote, ProCite, Reference Manager, RefWorks, Citavi, etc.)

BibTeX

Citation

CSV (for Excel, etc)

Export:

This page

Selected itens

All references (max. 2000)

Sem resultados

No documents were found for your search

Glossary and search help

You can enrich your search in a very simple way. Use the search indexes combined with the connectors (AND or OR) and specify more your search.

For example, if you want to search for articles about
cases of dengue in Brasil in 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

See below the complete list of search indexes that can be used:

Index code	Element
ti	article title
au	author
kw	article keywords
subject	subject (title words, abstract and keywords)
ab	abstract
ta	journal short title (e.g. Cad. Saúde Pública)
journal_title	journal full title (e.g. Cadernos de Saúde Pública)
la	publication language code (e.g. pt - Portuguese, es - Spanish)
type	document type
pid	publication identifier
publication_year	publication year of publication
sponsor	sponsor
aff_country	country code of the author's affiliation
aff_institution	author affiliation institution
volume	article volume
issue	article issue
elocation	elocation
doi	DOI number
issn	journal ISSN
in	SciELO colection code (e.g. scl - Brasil, col - Colômbia)
use_license	article usage license code

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
Email: scielo@scielo.org

All the contents of this site www.scielo.br, except where otherwise noted, is licensed under a Creative Commons Attribution License.
more »