Resumo Embora a qualidade do serviço e a satisfação do cliente tenham sido frequentemente discutidas na investigação turística, as suas conexões com fatores emocionais raramente têm sido investigadas num contexto de museu. O Museu de Antropologia de Guangxi foca-se em minorias étnicas únicas, e é usado como estudo de caso neste artigo. Este estudo examina as relações entre os fatores que influenciam o apego emocional, a satisfação, a lealdade conativa e a qualidade percebida dos visitantes. A modelagem de equações estruturais (PLS-SEM) foi utilizada para modelar as relações entre esses quatro construtos, tendo sido avaliada uma amostra de 184 visitantes do museu. O modelo conceitual suporta todas as hipóteses propostas. Os resultados mostram influências positivas entre o apego emocional, a satisfação dos visitantes e a qualidade percebida, o que pode de fato afetar a lealdade conativa dos visitantes a um museu específico.

Abstract Although service quality and customer satisfaction have been frequently discussed by scholars in tourism research, their connections with emotional factors have rarely been investigated in a museum context. The Anthropology Museum of Guangxi focuses on unique ethnic minorities, which is used as the case study in this paper. This study examines the relationships among the factors that influence visitors’ emotional attachment, satisfaction, conative loyalty, and perceived quality. Structural equation modeling (PLS-SEM) was used to model the relations among these four constructs, a 184-visitor sample is assessed, and the conceptual model supports all the proposed hypotheses. The results show positive influences among emotional attachment, visitor satisfaction, and perceived quality, which may indeed affect visitors’ conative loyalty to a specific museum. The practical applications are suggested based on findings that the conative loyalty of museum visitors is relatively influenced by their emotional connection and that emotional arousal can promote the acceptance of museum tourism.

ABSTRACT This study evaluated the effect of fermented soybean meal (FSBM) supplementation on growth performance, meat quality, blood biochemical parameters, and fecal microflora of finishing pigs. Thirty-two crossbred pigs (Duroc × Landrance × Yorkshire) (66-day-old, 67.95±0.25 kg) were randomly allocated to two treatments and fed diets containing soybean meal and FSBM. The average daily gain (ADG), average daily feed intake (ADFI), feed conversion ratio (FCR), blood biochemical parameters, and meat quality index were measured. At the end of experiment, the fecal microflora of finishing pigs was analyzed with 16S rDNA techniques. Results revealed that pigs fed FSBM had a greater ADG and lower cooking loss relative to control group (basal diets). Compared with the control group, the triglyceride content in the serum of the group fed FSBM increased significantly, and the creatinine content in the serum decreased notably. Fermented soybean meal enhanced the abundance of Bacteroidetes, Prevotellaceae, Bcteroidales, Bacteroidia, but inhibited the growth of Firmicutes, Clostridia, Clostridiales, and Ruminococcaceae in the intestine of pigs. Therefore, we can speculate that FSBM may play an important role in animal production. Dietary FSBM supplementation may be beneficial to some aspects of growth performance and the diversity of fecal microflora in finishing pigs.

OBJECTIVES: Cerebral ischemia seriously threatens human health and is characterized by high rates of incidence, disability and death. Developing an ideal animal model of cerebral ischemia that reflects the human clinical features is critical for pathological studies and clinical research. The goal of this study is to establish a local cerebral ischemia model in rhesus macaque, thereby providing an optimal animal model to study cerebral ischemia. METHODS: Eight healthy rhesus monkeys were selected for this study. CT scans were performed before the operation to exclude cerebral vascular and intracranial lesions. Under guidance and monitoring with digital subtraction angiography (DSA), a microcatheter was inserted into the M1 segment of the middle cerebral artery (MCA) via the femoral artery. Then, autologous white thrombi were introduced to block blood flow. Immediately following embolization, multisequence MRI was used to monitor cerebrovascular and brain parenchymal conditions. Twenty-four hours after embolization, 2 monkeys were sacrificed and subjected to perfusion, fixation and pathological examination. RESULTS: The cerebral ischemia model was established in 7 rhesus monkeys; one animal died during intubation. DSA and magnetic resonance angiography (MRA) indicated the presence of an arterial occlusion. MRI showed acute local cerebral ischemia. HE staining revealed infarct lesions formed in the brain tissues, and thrombi were present in the cerebral artery. CONCLUSION: We established a rhesus macaque model of local cerebral ischemia by autologous thrombus placement. This model has important implications for basic and clinical research on cerebral ischemia. MRI and DSA can evaluate the models to ensure accuracy and effectiveness.

ABSTRACT Purpose: To compare two-shift operation mode and single player mode different impact on surgical results and operator comfort in flexible ureteroscopic holmium laser lithotripsy for renal calculi larger than 1.5cm. Materials and Methods: From december 2017 to december 2018, 92 patients with renal calculi admitted to Qilu Hospital and were treated through flexible ureteroscopy. They were randomized in two-shift group (n=50) and single player group (n=42). The operative time, blood loss, hospitalization stay after operation, residual fragments (≥4mm) rate, fragmentation speed, postoperative complications and operator's fatigue score were compared. Results: There was no significant difference between two groups regarding age, gender, illness side, stone size, blood loss, operative time, postoperative hospitalization stay, complications, etc (p >0.05). The fragmentation speed was 44.5±20.0mm3/min in two-shift group compared with 34.2±17.3mm3/min in single player group (p=0.037). Residual fragments (≥4mm) rate after first surgery was 18% in two-shift group, while the residual fragments (≥4mm) rate was 40.5% after first surgery in single player group (p=0.017). The total fatigue score of two-shift group was 8.4 compared to 29.9 in single player group (p <0.001). Conclusion: In flexible ureteroscopic holmium laser lithotripsy for the treatment of renal calculi larger than 1.5cm, two-shift operation mode can raise the fragmentation speed and stone clearance rate, as well as significantly lower operator's fatigue level and improve operator's comfort.

Abstract Three-dimensional numerical simulations and experiments were performed to examine the formation and spatial dispersion patterns of integral multiple explosively formed penetrators (MEFP) warhead with seven hemispherical liners. Numerical results had successfully described the formation process and distribution pattern of MEFP. A group of penetrators consisting of a central penetrator surrounded by 6 penetrators is formed during the formation process of MEFP and moves in the direction of aiming position. The maximum divergence angle of the surrounding penetrator group was 7.8°, and the damage area could reach 0.16 m2 at 1.2 m. The laws of perforation dispersion patterns of MEFP were also obtained through a nonlinear fitting of the perforation information on the target at different standoffs. The terminal effects of the MEFP warhead were performed on three #45 steel targets with a dimension of 160cm ( 160cm ( 1.5cm at various standoffs (60, 80, and 120 cm). The simulation results were validated through penetration experiments at different standoffs. It has shown excellent agreement between simulation and experiment results.

The central carbon metabolic system is the upstream energy source for microbial fermentation. In addition, it is a master switch for increasing the production of metabolites and an important part of the microbial metabolic network. Investigation into the relationship between genes, environmental factors, and metabolic networks is a main focus of systems biology, which significantly impacts research in biochemistry, metabolic engineering, and synthetic biology. To this end, the central carbon metabolic flux under a variety of growth conditions or using strains with various genetic modifications was previously measured in Saccharomyces cerevisiae using 13C tracer technology. However, the measured values were not integrated and investigated further. In this study, we collected and analyzed the metabolic flux rates of the central carbon metabolic system in S. cerevisiae measured in recent studies. We carried out preliminary analyses of flux values of each pathway, performed regression analyses on relationship between different fluxes, and extracted principal component factors of the flux variables. Based on the results, the general characteristics of pathway flux distribution were clustered and explored, and the effects of environmental and genetic factors on the flux distribution were analyzed. Furthermore, this study explored the relationship between similarity in the enzyme's transcriptional regulation and the correlations in the enzyme's reaction flux. Our results provide a foundation for further studies on the control of the central carbon metabolic flux and facilitate the search for targets in metabolic engineering research.

This study aims to explore the role of palatal rugae pattern in forensic identification by coding of palatal rugae characteristics, and to construct a forensic identification system for oral palatal rugae. One hundred models were included in this study for a systemic coding of palatal rugae pattern based on the shape, quantity, location, and distribution of palatal rugae. Among the involved 100 models, palatal rugae types varied among individuals and palatal rugae pattern was different between men and women, even between two sides in the same individual. Palatal rugae pattern can be used for forensic identification of oral soft tissue and this study proposes a new means for the identification by coding of palatal rugae pattern based on the shape, quantity, location and distribution.

Este estudio tuvo como objetivo explorar el patrón de rugas palatinas en la identificación forense mediante la codificación de las características de éstas. Además se elaboró un sistema de identificación forense. Fueron incluidos cien modelos para una codificación sistemática del patrón de rugas palatinas basado en su forma, cantidad, ubicación y distribución. En todos los modelos se identificó una variación entre los individuos y en el patrón entre hombres y mujeres. El patrón de rugas palatinas puede ser utilizado para la identificación forense de tejido blando oral y este estudio propone un método nuevo para codificarlas.

The kinetics of oxidation of pentaerythritol by ditelluratoargentate (III) (DTA) in alkaline liquids has been studied spectrophotometrically in the temperature range of 293.2 K-313.2 K. The reaction rate showed first-order dependence in DTA and fractional order with respect to pentaerythritol. It was found that the pseudo-first-order rate constant k obs increased with an increase in concentration of OH- and a decrease in concentration of H4TeO6²־. There was a negative salt effect and no free radicals were detected. A plausible mechanism involving a two-electron transfer is proposed and the rate equations derived from the mechanism can explain all the experimental results. The rate equations derived from mechanism can explain all experimental observations. The activation parameters along with the rate constants of the rate-determining step were calculated.