Abstract Emerging studies indicate the persistence of symptoms beyond the acute phase of COVID-19. Cognitive impairment has been observed in certain individuals for months following infection. Currently, there is limited knowledge about the specific cognitive domains that undergo alterations during the post-acute COVID-19 syndrome and the potential impact of disease severity on cognition. The aim of this review is to examine studies that have reported cognitive impairment in post-acute COVID-19, categorizing them into subacute and chronic phases. The methodology proposed by JBI was followed in this study. The included studies were published between December 2019 and December 2022. The search was conducted in PubMed, PubMed PMC, BVS – BIREME, Embase, SCOPUS, Cochrane, Web of Science, Proquest, PsycInfo, and EBSCOHost. Data extraction included specific details about the population, concepts, context, and key findings or recommendations relevant to the review objectives. A total of 7,540 records were identified and examined, and 47 articles were included. The cognitive domains most frequently reported as altered 4 to 12 weeks after acute COVID-19 were language, episodic memory, and executive function, and after 12 weeks, the domains most affected were attention, episodic memory, and executive function. The results of this scoping review highlight that adults with post-acute COVID-19 syndrome may have impairment in specific cognitive domains.

Resumo Estudos emergentes indicam a persistência dos sintomas além da fase aguda da COVID-19. O comprometimento cognitivo foi observado em alguns indivíduos durante meses após a infecção. Atualmente, há pouco conhecimento sobre os domínios cognitivos específicos que sofrem alterações durante a síndrome pós-aguda da COVID-19 e o possível impacto da gravidade da doença na cognição. O objetivo desta revisão é examinar estudos que relataram comprometimento cognitivo na COVID-19 pós-aguda, categorizando-os em fases subaguda e crônica. A metodologia proposta pela Joanna Briggs Institute foi seguida neste estudo. Os estudos incluídos foram publicados entre dezembro de 2019 e dezembro de 2022. A busca foi realizada no PubMed, PubMed PMC, BVS – BIREME, Embase, SCOPUS, Cochrane, Web of Science, Proquest, PsycInfo e EBSCOHost. A extração de dados incluiu detalhes específicos sobre a população, os conceitos, o contexto e as principais descobertas ou recomendações relevantes para os objetivos da revisão. Um total de 7.540 registros foi identificado e examinado, e 47 artigos foram incluídos. Os domínios cognitivos mais frequentemente relatados como alterados de 4 a 12 semanas após a COVID-19 aguda foram linguagem, memória episódica e função executiva e, após 12 semanas, os domínios mais afetados foram atenção, memória episódica e função executiva. Os resultados dessa revisão de escopo destacam que adultos com síndrome pós-aguda da COVID-19 podem apresentar comprometimento em domínios cognitivos específicos.

Abstract Objectives: To screen the COL1A1 and COL1A2 gene mutation sites in a family with type I osteogenesis imperfecta (OI)/hearing loss and analyze the characteristics and recovery of hearing loss in patients with osteogenesis imperfecta. Methods: The basic clinical data of Ol proband and her parents were collected, and the COL1A1 and COL1A2 genes were detected in peripheral blood by PCR amplification and generation Sanger sequencing. Literature of stapedial surgery in patients with osteogenesis imperfecta was collected. Results: The heterozygous mutation of the 26 exon c.1922_1923 ins C in the Ol progenitor COL1A1 gene led to the amino acid frameshift mutation of p.Pro 601FS, which was not detected in the phenotypic parents. The homozygous of exon 28 c.1782>G in COL1A2 was detected in the proband and her parents, resulting in changes in the protein p.Pro 549Ala. Conclusion: The clinical symptoms of the Ol proband is caused by heterozygous mutation of the 26 exon c.1922_1923 ins C in COL1A1 gene. Stapedial surgery can provide short-term and long-term hearing benefits for Ol patients with hearing loss. Level of evidence: Level 4.

The low-temperature electrochemical corrosion behavior of aircraft 2024 aluminum alloy was investigated in different concentrations of potassium-acetate-type fluid, and the results showed that the corrosion medium initially impacted some corrosiveness on the 2024 aluminum alloy. However, the corrosion rate of the alloy decreased with prolonged immersion in the corrosion medium, and this was attributed to the protective effect of the corrosion products layer, which impeded the attack by the aggressive solution. Time-domain analysis of electrochemical noise measurements at -5 °C revealed the occurrence of uniform corrosion on the alloy at the initial stages of immersion, general corrosion at the middle stages of immersion, and a pitting-dominated corrosion type from the middle to late stages of immersion.

Abstract: The co-existence of external referencing pricing (ERP) and reimbursement policy is common in many countries. Thus, this research examines whether or not the imposition of ERP is socially desirable in the presence of reimbursement policy. For direct sales channel, we find that the home social welfare is worse-off with ERP if the home copayment rate is too high. Our main results are robust under indirect sales channel. Moreover, the home social welfare under the pharmacypurchasing-price (PPP) ERP is larger than that under the ex-factory-price (EFP) ERP if the home copayment rate is high enough. Finally, the profit of brand-name firm under indirect sales channel is higher than that under direct sales channel if the home copayment rate is too high.

Resumen: Precios referenciales externos (ERP) y políticas de reembolso coexisten en muchos países. Este documento evalúa si ERP son socialmente deseables en presencia de políticas de reembolso. Para el canal de venta directa, encontramos que el bienestar social del hogar está peor con ERP si la tasa de copago de la vivienda es demasiado alta. Nuestros principales resultados son robustos bajo el canal de ventas indirectas. Además, el bienestar social bajo el precio de compra de farmacia (PPP) es mayor que el del ERP de precio de fábrica (EFP) si la tasa de copago es lo suficientemente alta. Finalmente, el beneficio de la empresa de marca en el canal de ventas indirectas es mayor que el del canal de ventas directas si la tasa de copago es demasiado alta.

Resumen La nefropatía obstructiva se considera una manifestación inusual en las vasculitis ANCA. Se presenta el caso de un masculino de 38 años, con granulomatosis con poliangitis e hidronefrosis unilateral, y revisión de la literatura. Masculino de 38 años, sano, quien consulta por cuadro subagudo de odinofagia, síntomas constitucionales y lesión renal aguda anúrica. Inicialmente con hallazgo de hidronefrosis izquierda, manejado como nefropatía obstructiva, que eventualmente desarrolla hemorragia alveolar difusa, distrés respiratorio y fallece debido a un síndrome de distrés respiratorio agudo severo refractario asociado a su granulomatosis con poliangitis. La nefropatía obstructiva es una manifestación inusual de las vasculitis ANCA asociadas. Es importante la sospecha diagnóstica en estos cuadros multisistémicos para no dilatar el tratamiento inmunosupresor conjunto con el resto de las terapias requeridas.

Abstract Obstructive nephropathy is considered an unusual presentation in ANCA-associated vasculitis. The following case describes a 38-year-old male with granulomatosis with polyangiitis and unilateral hydronephrosis, as well as a literature review. A 38-year-old male with an unremarkable medical background presents with a 3-week history of odynophagia, constitutional symptoms and anuric kidney injury. Initially managed as an obstructive nephropathy due to a left hydronephrosis finding, the patient eventually develops a diffuse alveolar hemorrhage, acute respiratory distress and perishes due to granulomatosis with polyangiitis. Obstructive nephropathy is an unusual manifestation of ANCA-associated vasculitis. Diagnostic suspicion is important in these multisystem pictures so as not to delay immunosuppressive treatment together with the rest of the required therapies.

Abstract The brewing of Chinese Baijiu is a microbial community fermentation system, in which there is a close and complex relationship between microorganisms and flavor quality, but the specific deep-seated mechanism still needs to be explored and interpreted continuously so far. In this review, Maotai flavor Baijiu was briefly introduced, and the microorganisms mainly affected by flavor substances, the main components of flavor substances, the factors affecting flavor formation and the application ways of functional microorganisms in Maotai flavor Baijiu were summarized. It provides a deeper understanding for the research of others flavor Baijiu.

RESUMO Introdução A coreografia das artes marciais é um evento esportivo de alta intensidade e velocidade. O metabolismo energético de seus praticantes é caracterizado principalmente pelo consumo anaeróbico, complementado pelo metabolismo aeróbico. As rotinas das artes marciais têm suas próprias regras de movimento particulares e o método de treinamento sistemático tem desempenhado um papel positivo no desenvolvimento das rotinas das artes marciais. Objetivo Este artigo explora o papel do treinamento sistemático por 8 semanas nas rotinas de artes marciais. Métodos 36 jogadores de coreografia em artes marciais de faculdades e universidades foram selecionados por amostragem aleatória. Os sujeitos da pesquisa acima foram divididos aleatoriamente em dois grupos: experimental e controle. O grupo experimental adotou um protocolo exclusivo de treinamento. O grupo de controle foi treinado da maneira tradicional. Resultados Foram encontradas diferenças significativas entre os dois grupos nos resultados dos testes de oito indicadores de velocidade. Também se observou diferença significativa entre os dois grupos nos dados de teste dos três indicadores de resistência. Conclusão O método personalizado de treinamento utilizado neste trabalho demonstrou um efeito de destaque na melhoria da velocidade e do nível de resistência dos atletas. Os resultados da pesquisa deste artigo podem fornecer uma referência para a equipe de rotina de artes marciais no aprimoramento do nível técnico profissional atlético. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction Martial arts choreography is a sporting event of high intensity and speed. The energy metabolism of its practitioners is characterized mainly by anaerobic consumption, complemented by aerobic metabolism. Martial arts routines have particular movement rules, and the systematic training method has played a positive role in developing martial arts routines. Objective This paper explores the role of eight-week systematic training in martial arts routines. Methods 36 martial arts choreography players from colleges and universities were selected by random sampling. The above research subjects were randomly divided into experimental and control groups. The experimental group adopted an exclusive training protocol. The control group was trained traditionally. Results Significant differences were found between the two groups in the test results of eight-speed indicators. A significant difference was also found between the two groups in the test data of the three endurance indicators. Conclusion The personalized training method used in this paper significantly improved athletes’ speed and endurance levels. The research results of this paper can provide a reference for routine martial arts staff in improving the professional athletic technical level. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción La coreografía de las artes marciales es un evento deportivo de alta intensidad y velocidad. El metabolismo energético de sus practicantes se caracteriza principalmente por el consumo anaeróbico, complementado por el metabolismo aeróbico. Las rutinas de las artes marciales tienen sus propias reglas de movimiento y el método de entrenamiento sistemático ha desempeñado un papel positivo en el desarrollo de las rutinas de las artes marciales. Objetivo Este trabajo explora el papel del entrenamiento sistemático durante 8 semanas en las rutinas de las artes marciales. Métodos Se seleccionaron 36 coreógrafos de artes marciales de colegios y universidades mediante un muestreo aleatorio. Los sujetos de la investigación mencionados se dividieron aleatoriamente en dos grupos: experimental y de control. El grupo experimental adoptó un protocolo de entrenamiento exclusivo. El grupo de control fue entrenado de forma tradicional. Resultados Se encontraron diferencias significativas entre los dos grupos en los resultados de las pruebas de ocho indicadores de velocidad. También se observó una diferencia significativa entre los dos grupos en los datos de las pruebas de los tres indicadores de resistencia. Conclusión El método de entrenamiento personalizado utilizado en este trabajo demostró un efecto destacado en la mejora del nivel de velocidad y resistencia de los atletas. Los resultados de la investigación de este trabajo pueden proporcionar una referencia para el equipo de artes marciales de rutina en la mejora del nivel técnico atlético profesional. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Abstract Dendrobium nobile is a traditional Chinese medicine and functional food in China, with anti-aging, immunity-enhancing, glucose-lowering and lipid-lowering health functions. In recent years, the research value of D. nobile has attracted the attention of more and more experts and scholars. Today, phytochemical investigation of the stems of Dendrobium nobile Lindl., led to the isolation of five sesquiterpene glycosides, including dendromoniliside D (1), dendronobiloside A (2), dendronobiloside C (3), dendronobiloside D (4), dendroside G (5) and their α-amylase and α-glycosidase inhibitory activities were also investigated. Among them, dendromoniliside D (1), dendroside G (5) were first isolated from D. nobile. And dendronobiloside A (2) and dendronobiloside C (3) exhibited better inhibitory activity against α-glycosidases, respectively. In the case of α-amylase inhibition, dendroside G (5) has a relatively good effect. These results suggest that sesquiterpene glycosides of D. nobile have potential hypoglycemic activity and deserve further research and development.

Abstract Burley tobacco-leaf protein extracted by alkali and precipitated by acid was hydrolyzed enzymatically, and optimum protease and hydrolysis parameters were determined. Pepsin was best and gave the highest hydrolysis rate (15.55%). As per results of the single-factor test, optimum hydrolysis conditions were: temperature 37 °C, pH 2, and an [E]/[S] ratio of 5%. These hydrolysis conditions were optimized by an orthogonal experiment that rendered the same results as the single factor experiment. The ratio of essential to total amino acids and that of essential to non-essential amino acids contained in the burley tobacco-leaf protease hydrolysate were higher than the FAO/WHO standard values of 0.4 and 0.6, among which hydrophobic amino acid content was the highest, followed by negatively and positively charged amino acid contents. In vitro antioxidant experiments showed that the scavenging rates of DPPH, superoxide anion, and hydroxyl radicals of tobacco-leaf protein hydrolysates were higher than those before hydrolysis. Further, the hydroxyl radical-scavenging rate was the most significant, and total antioxidant capacity was improved. The nutritive value of the burley tobacco-leaf protein hydrolysate improved upon pepsin-mediated hydrolysis, and the antioxidant activity was better. These results provide a theoretical basis for further development and utilization of tobacco protein resources.

Abstract Background Alpha-kinase 1 (ALPK1) is a master regulator in inflammation and has been proved to promote renal fibrosis by promoting the production of IL-1β in diabetic nephropathy (DN) mice. Pyroptosis is involved in high glucose (HG)-induced tubular cells injury, characterized by activation of Gasdermin D (GSDMD) and the release of IL-1β and IL-18, resulting in inflammatory injury in DN. It is reasonable to assume that ALPK1 is involved in pyroptosis-related tubular injury in DN. However, the mechanism remains poorly defined. Methods Immunohistochemistry (IHC) staining was performed to detect the expression of pyroptosis- and fibrosis-related proteins in renal sections of DN patients and DN mice. DN models were induced through injection of streptozotocin combined with a high-fat diet. Protein levels of ALPK1, NF-κB, Caspase-1, GSDMD, IL-1β, IL-18 and α-SMA were detected by Western blot. HK-2 cells treated with high-glucose (HG) served as an in vitro model. ALPK1 small interfering RNA (siRNA) was transfected into HK-2 cells to down-regulate ALPK1. The pyroptosis rates were determined by flow cytometry. The concentrations of IL-1β and IL-18 were evaluated by ELISA kits. Immunofluorescence staining was used to observe translocation of NF-κB and GSDMD. Results The heat map of differentially expressed genes showed that ALPK1, Caspase-1 and GSDMD were upregulated in the DN group. The expression levels of ALPK1, Caspase-1, GSDMD and CD68 were increased in renal biopsy tissues of DN patients by IHC. ALPK1expression and CD68+ macrophages were positively correlated with tubular injury in DN patients. Western blot analysis showed increased expressions of ALPK1, phospho-NF-κB P65, GSDMD-NT, and IL-1β in renal tissues of DN mice and HK-2 cells, accompanied with increased renal fibrosis-related proteins (FN, α-SMA) and macrophages infiltration in interstitial areas. Inhibition of ALPK1 attenuated HG-induced upregulation expressions of NF-κB, pyroptosis-related proteins Caspase-1, GSDMD-NT, IL-1β, IL-18, α-SMA, and pyroptosis level in HK-2 cells. Also, the intensity and nuclear translocation of NF-κB and membranous translocation of GSDMD were ameliorated in HG-treated HK-2 cells after treatment with ALPK1 siRNA. Conclusions Our data suggest that ALPK1/NF-κB pathway initiated canonical caspase-1-GSDMD pyroptosis pathway, resulting in tubular injury and interstitial inflammation of DN.

Abstract Dendrobium officinale is a famous traditional Chinese medicine and nourishing food in China. The active constituents in D. officinale include a variety of chemical components such as polysaccharides, bibenzyl, phenanthrenes, and flavonoids etc. Among them, polysaccharide was selected as the quality control marker for D. officinale in the Chinese Pharmacopoeia. The previous studies found that the content of polysaccharide constituents in D. officinale from different growing origins had significant differences. To the best of our knowledge, whether the secondary metabolites other than polysaccharides are similarly different in D. officinale from different origins has not been reported. In the present continuing study, UPLC-Q/TOF-MS coupled with multivariate analysis were employed for comparative analysis of the secondary metabolites of D. officinale from different growing origins. At last, 12 differential secondary metabolites of D. officinale from different origins were identified. Network pharmacological analysis results indicated that these differential secondary metabolites of D. officinale from different origins are mainly involved in neuropsychiatric related disorder diseases. This investigation will provide a valuable information for doctors and consumers in clinical practice.

Abstract Kaempferol, an important flavonol, has numerous health-beneficial bioactivities and possesses a great potential for application in medicine, food, and cosmetics industries. To improve the production of kaempferol in an in vitro synthetic biosystem, we designed and constructed a panel of bifunctional enzymes by fusing the flavanone 3-hydroxylase (AtF3H) and the flavonol synthase (AtFLS1) of Arabidopsis thaliana with different orientation and different peptide linker type and length. By comparing the output of kaempferol, we obtained a highly active bifunctional enzyme AtF3H-(GGGGS)2-AtFLS1 with a Km value of 0.129 ± 0.016 mM. After optimization of a series of reaction parameters, kaempferol was produced at 100.54 ± 0.54 mg/L, the currently highest kaempferol output in an in vitro synthetic biosystem, and the substrate conversion rate was 68.26% ± 0.05%. In addition, we observed substrate inhibition for the AtFLS1, which eventually limited the production of kaempferol. This study provides a highly active biocatalyst for production of kaempferol and an insight into biosynthesis of other valuable molecules.

This study evaluated the effects of perioperative nutrition management by a multidisciplinary team on nutrition and postoperative complications of patients with esophageal cancer. A total of 239 patients with esophageal cancer who underwent esophagectomy and gastric conduit reconstruction for esophageal or esophagogastric junction cancer between February 2019 and February 2020 were included in the study. They were divided into the experimental group (120 patients) and the control group (119 patients) using the random number table method. Control group patients received routine diet management and experimental group patients received perioperative nutrition management by a multidisciplinary team. The differences of nutriture and postoperative complications between the two groups were compared. At 3 and 7 days after surgery, the experimental group patients had higher total protein and albumin levels (P<0.05), shorter postoperative anal exhaust time (P<0.05), lower incidence of postoperative gastrointestinal adverse reactions, pneumonia, anastomotic fistula, hypoproteinemia (P<0.05), and lower hospitalization costs (P<0.05) than the control group. Nutrition management by a multidisciplinary team effectively improved the nutriture of patients, promoted the rapid recovery of postoperative gastrointestinal function, reduced postoperative complications, and reduced hospitalization costs.

Abstract The Kaxinawá indigenous people (auto-identified as the Huni Kuĩ) are native to the western Amazon, on the Brazilian border with Peru, and have an extensive biodiversity-related knowledge, which is parti of a coherent social-ecological system. Our study investigated native knowledge of edible forest plants, in three communities within the Kaxinawá Indigenous Land on the Lower Rio Jordão, Acre state, Brazil. The principal methods used were participant observation, open and semi-structured interviews and walk-in-the-woods. The study recorded 89 native food species. Some 60% are managed in food-production areas, with 56% of the recorded species have received little or no scientific study of their food potential. The role which natural systems play in Huni Kuĩ society is indicated by the management and use of a high diversity of native species, which contributes to food security and nutritional sovereignty. However, there is a progressive under-utilization and substitution of forest species, due to the introduction of cultivated exotic species and the increase in consumption of industrialized products. An enhancement of the value given to native food-plants is needed to encourage continuing autonomy of production, and diet diversification, as well as the conservation of sociobiodiversity of traditional peoples in the Amazon through sustainable management practices of the current social-ecological system.

Resumo O povo indígena Kaxinawá (auto-denominado Huni Kuĩ) é nativo da Amazônia ocidental, na fronteira brasileira com o Peru, e possuem um vasto conhecimento sobre a biodiversidade, formando um sistema sócio-ecológico consistente. O presente estudo investigou conhecimentos nativos de plantas florestais alimentícias, em três comunidades da Terra Indígena Kaxinawá no Baixo Rio Jordão, estado do Acre, Brasil. As principais metodologias utilizadas foram: observação participante, entrevistas abertas e semiestruturadas e trilhas-guiadas na floresta e nos agroecossistemas. O estudo registou 89 espécies alimentícias nativas. Dessas, cerca de 60% são manejadas igualmente em áreas de produção alimentar. Ao mesmo tempo, aproximadamente 56% das espécies possuem pouco ou nenhum estudo científico sobre o seu potencial alimentar. O papel que os sistemas naturais desempenham na sociedade Huni Kuĩ é indicado pelo uso e manejo de uma grande diversidade de espécies nativas, o que contribui para a segurança e a soberania alimentar e nutricional. Contudo, há uma progressiva subutilização e substituição de espécies florestais, devido à introdução de espécies exóticas cultivadas e ao aumento do consumo de produtos industrializados. Nesse sentido, é importante valorizar as plantas alimentares nativas a fim de promover a contínua autonomia de produção e diversificação alimentar, bem como a conservação da sociobiodiversidade dos povos tradicionais na Amazônia, por meio de práticas de manejo sustentável do atual sistema socioecológico.