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ABSTRACT The expression ‘you need to know to conserve’ is a well-known cliche among biologists. Documenting the richness of a group of organisms is the first step towards understanding biodiversity and preparing efficient conservation plans. In this context, many efforts have been made to quantify the number of species on Earth and estimate the number of species still unknown to science. A few countries have complete and integrated databases estimating the approximate number of species recorded for their territory, particularly in the Global South. In Brazil, a country of continental dimensions, revealing the richness of the second most diverse clade of invertebrates (=Mollusca) has been a goal of taxonomists. Recently, in an unprecedented, collective, and integrated effort among Brazilian malacologists, it was possible to estimate how many valid species of molluscs are there in Brazil. In this effort, more than 30 mollusc experts joined together to update the Taxonomic Catalogue of the Brazilian Fauna (TCBF), a governmental website that allows a quick and real-time updating of all Metazoan. So far, more than 5,000 updates have been made in TCBF, indicating the presence of 3,552 valid species of molluscs in Brazil, distributed among the main clades as follows: Caudofoveata (10 spp.), Solenogastres (6 spp.), Polyplacophora (35 spp.), Scaphopoda (43 spp.), Cephalopoda (92 spp.), Bivalvia (629 spp.) and Gastropoda (2,737 spp.). The present study, in addition to demonstrating for the first time the richness of Brazilian molluscs, also presents the state of the art of this important phylum of invertebrates highlighting its most representative and neglected groups.

https://doi.org/10.1590/s1984-4689.v40.e23026

Cutaneous metastatic melanoma in a Siberian tiger (Panthera tigris altaica) - case report
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Eckstein, C.

; Tinoco, H.P.

; Coelho, C.M.

; Lima, P.A.

; Rocha, C.E.V.

; Santos, R.L.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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may 2020, Volume 72 N. 3 Pages 921 - 925

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Um tigre-siberiano (Panthera tigris altaica) de cativeiro, macho, de 15 anos de idade, apresentou hipermetria dos membros pélvicos nos últimos 10 anos, e recentemente, foi observado um nódulo preto e ulcerado caudalmente à orelha direita. O animal foi submetido à cirurgia para remoção do nódulo e morreu durante a recuperação anestésica. À necropsia, outro nódulo infiltrativo, foi detectado caudalmente a orelha direita. Histologicamente, ambos os nódulos correspondiam à neoplasia melanocítica, com células variando de fortemente pigmentadas a amelanóticas, com metástase para linfonodos mediastinais, baço e pulmão. Havia lipofucsinose e corpora amilácea no sistema nervoso central. Os achados macroscópicos e histológicos confirmam o diagnóstico de melanoma cutâneo metastático em um tigre-siberiano adulto de cativeiro.

ABSTRACT A male 15-year-old captive Siberian tiger (Panthera tigris altaica) developed pelvic limb hypermetry over the past 10 years. Recently, an ulcerated black nodule located caudally to the right ear was observed. The animal was submitted to surgery for removing the tumor, but died during anesthetic recovery. At necropsy, another infiltrative nodule was observed caudally to the right ear. Histologically, both nodules corresponded to melanocytic neoplasia, varying from heavily pigmented to amelanotic, with metastasis to mediastinal lymph nodes, spleen and lung. Lipofuscinosis and corpora amylacea were histologically observed in the central nervous system. Macroscopic and histologic findings confirmed the diagnosis of skin metastatic melanoma in a captive adult Siberian tiger.

https://doi.org/10.1590/1678-4162-10319 811 downloads

Genetic diversity and nonparametric statistics to identify possible ISSR marker association with fiber quality of pineapple
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SILVA, JULIANNA M.

; LIMA, PAULO R.L.

; SOUZA, FERNANDA V.D.

; LEDO, CARLOS A.S.

; SOUZA, EVERTON H.

; PESTANA, KATIA N.

; FERREIRA, CLÁUDIA F.

Anais da Academia Brasileira de Ciências

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2019, Volume 91 N. 3 elocation e20180749

Abstract: En | Text: En | PDF: En

Abstract: Due to the increasing search for renewable resources, plant fibers have become an alternative when creating new products. Studies demonstrate the potential use of pineapple fibers in composites. The objective of this work was to evaluate the genetic diversity and verify any association between ISSR (Inter Simple Sequence Repeats) bands and quality of pineapple fibers for use in cements in the civil construction. The study analyzed the genetic variability of 11 pineapple genotypes, as well as the possible association of 131 bands from 16 ISSR markers with fiber quality characteristics. Eleven bands were selected based on their high correlations (0.64578* to 0.72457*) with three fiber quality variables. Of these, two bands were purified, sequenced, and blasted against sequences in GenBank at NCBI. These markers can be used in marker assisted selection to genetically improve the quality of pineapple fiber. Bands that returned no hits in the NCBI BLAST search can be deposited as new sequences in the GenBank. Therefore, the SCAR markers, once validated, can be useful in pineapple genetic breeding programs worldwide by using molecular marker assisted selection for fiber resistance, which could subsidize the development of more promising genotypes for industrial use and contribute to the sustainability of this new production sector.

https://doi.org/10.1590/0001-3765201920180749 970 downloads

Estudo retrospectivo da casuística de felinos domésticos no Hospital Veterinário da Universidade Federal de Minas Gerais, no período de 2005 a 2014
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Lima, P.A.

; Barbieri, J.M.

; Ecco, R.

; Guedes, R.M.C.

; Serakides, R.

; Ocarino, N.M.

; Pierezan, F.

; Santos, R.L.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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dec 2018, Volume 70 N. 6 Pages 1775 - 1783

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste estudo foi caracterizar as doenças dos gatos domésticos provenientes dos casos de necropsia e histopatologia do Hospital Veterinário da Universidade Federal de Minas Gerais (HV/UFMG), de 2005 a 2014. Foram analisados 408 exames de necropsia e 197 de biópsias, segundo o sexo, a faixa etária e a raça. Os diagnósticos mais frequentes incluíram doenças infecciosas/inflamatórias ou parasitárias (22,5%), agentes físicos (18,1%), doenças proliferativas (15,2%) e degenerativas (13,5%). Politraumatismo (10,8%) foi responsável pelo maior número de mortes em felinos, sendo as chances três vezes maiores em animais com até 24 meses de idade (P=0,005 OR 3,47 [IC 95%: 1,40-8,57]). Neoplasias epiteliais corresponderam a 26 diagnósticos, sendo 20 (4,9%) casos de malignidade. A ocorrência de carcinoma e seus subtipos foi 18 vezes maior em gatos idosos (P<0,01 OR 18,15 [IC 95%: 7,41-44,45]). A insuficiência renal crônica foi mais frequente em gatos com mais de 120 meses (P=0,01). Machos apresentaram nove vezes mais chances de desenvolver doenças do trato urinário inferior quando comparados às fêmeas (P=0,001 OR 9,50 [IC 95%: 2,78-32,48]). Em relação às biópsias, animais adultos a idosos foram 10 vezes mais representados (P<0,001 OR 10,8 [IC 95%: 3,22-36,79]).

ABSTRACT The aim of this study was to characterize the diseases of domestic cats based on necropsy and histopathological examinations at the Veterinary Hospital of the Universidade Federal de Minas Gerais (HV/UFMG) from 2005 to 2014. A total of 408 necropsy and 197 biopsy samples were analyzed according to gender, age, and breed. The most frequent diagnoses included infectious/inflammatory or parasitic diseases (22.5%), physical agents (18.1%), proliferative (15.2%), and degenerative (13.5%) diseases. Polytrauma (10.8%) was responsible for the highest number of feline deaths, with the odds three times higher in animals up to 24 months of age (P= 0.005 OR 3.47 [95% CI: 1.40-8.57]). Epithelial neoplasms corresponded to 26 diagnoses, with 20 (4.9%) malignant cases. The occurrence of carcinoma and its subtypes was 18-fold higher in older cats (P< 0.01 OR 18.15 [95% CI: 7.41-44.45]). Chronic renal failure was more frequent in cats over 120 months (P= 0.01). Males were nine times more likely to develop lower urinary tract diseases when compared to females (P= 0.001 OR 9.50 [95% CI: 2.78-32.48]). Regarding the biopsies, adult to elderly animals were ten times more represented (P< 0.001 OR 10.8 [95% CI: 3.22-36.79]).

https://doi.org/10.1590/1678-4162-9857 3645 downloads

New flavone and other compounds from Tephrosia egregia: assessing the cytotoxic effect on human tumor cell lines
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Teixeira, Maria V.S.

; Lima, Jefferson Q.

; Pimenta, Antônia T.A.

; Silva, Francisca R.L. da

; Oliveira, Maria da Conceição F. de

; Pereira, Italo G.

; Costa-Junior, Dilailson C.

; Ribeiro, Paulo R.V.

; Santiago, Gilvandete M.P.

; Lima, Mary Anne S.

; Braz-Filho, Raimundo

; Bezerra, Mirna M.

; Montenegro, Raquel C.

; Rocha, Danilo D.

; Moraes, Maria Elisabete A. de

; Medeiros, Aline C. de

; Arriaga, Angela M.C.

Revista Brasileira de Farmacognosia

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jun 2018, Volume 28 N. 3 Pages 333 - 338

Abstract: En | Text: En | PDF: En

ABSTRACT The organic extracts from stems, roots and leaves of Tephrosia egregia Sandwith, Fabaceae, provided a new flavone, 5-hydroxy-8-(1",2"-epoxy-3"-hydroxy-3"-methylbutyl)-7-methoxyflavone (1), in addition to eleven known compounds: pongaflavone (2), praecansone B (3), 12a-hydroxyrotenone (4), praecansone A, 2',6'-dimethoxy-4',5'-(2",2"-dimethyl)-pyranochalcone, pongachalcone, maackiain, β-sistosterol and its glucoside, p-cumaric acid and cinnamic acid. The structures of all compounds were established on the basis of spectroscopic methods, mainly 1D and 2D NMR and HRESIMS, involving comparison with literature data. Cytotoxicity of compounds 1–4 was evaluated against AGP-01 (cancerous ascitic fluid), HCT-116 (colon adenocarcinoma), HL-60 (leukemia), PC-3 (prostate carcinoma), SF-295 (glioblastoma) and SKMEL 28 (melanoma) cell lines.

https://doi.org/10.1016/j.bjp.2018.03.008 525 downloads

Placental osseous metaplasia associated with abortion in a mare: case report
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Lima, P.A.

; Eckstein, C.

; Ecco, R.

; Santos, R.L.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jan 2018, Volume 70 N. 1 Pages 53 - 56

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste relato é descrever um caso de metaplasia óssea em placenta equina, associada a aborto. Uma égua da raça Campolina, com aproximadamente 19 anos de idade, após múltiplas gestações e sem histórico de alterações reprodutivas, abortou feto com idade aproximada de seis meses. Não foram observadas alterações macroscópicas no feto e na placenta. Microscopicamente, havia substituição do mesênquima de vilosidades coriônicas por tecido ósseo bem diferenciado e parcialmente mineralizado (confirmada pela coloração de von Kossa). Para caracterização do componente colagenoso, foram realizadas colorações de tricrômico de Masson e picrosirius red. O tecido ósseo tinha predominância de colágeno do tipo I. Este é o primeiro relato de metaplasia óssea na placenta equina, associada a aborto no terço médio da gestação, presumivelmente devido à insuficiência placentária.

ABSTRACT The aim of this report is to describe a case of marked osseous metaplasia in the placenta associated with abortion in a mare. A 19-year-old Campolina mare with a history of multiple parturitions and absence of reproductive failure, aborted a fetus with approximately six months of gestation. There were no gross changes in the fetus and the placenta. Microscopically, there was multifocal replacement of fetal connective mesenchyme of the chorionic villi with well differentiated and partially mineralized osseous tissue (confirmed by von Kossa staining). To further characterize the collagenous component of the lesion, sections of the placenta were stained with Masson’s trichrome and Picrosirius red. Osseous tissue deposition had abundance of type I collagen. To the best of our knowledge, this is the first report of osseous metaplasia in the placenta of a mare associated with abortion at mid gestation, presumably due to placentary insufficiency.

https://doi.org/10.1590/1678-4162-9828 1614 downloads

Deficiency of sex hormones does not affect 17-ß-estradiol-induced coronary vasodilation in the isolated rat heart
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Santos, R.L.

; Lima, J.T.

; Rouver, W.N.

; Moysés, M.R.

Brazilian Journal of Medical and Biological Research

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2016, Volume 49 N. 5 elocation e5058

Abstract: En | Text: En | PDF: En

The relaxation of coronary arteries by estrogens in the coronary vascular beds of naive and hypertensive rats has been well described. However, little is known about this action in gonadectomized rats. We investigated the effect of 17-ß-estradiol (E2) in coronary arteries from gonadectomized rats, as well as the contributions of endothelium-derived factors and potassium channels. Eight-week-old female and male Wistar rats weighing 220-300 g were divided into sham-operated and gonadectomized groups (n=9−12 animals per group). The baseline coronary perfusion pressure (CPP) was determined, and the vasoactive effects of 10 μM E2 were assessed by bolus administration before and after endothelium denudation or by perfusion with NG-nitro-L-arginine methyl ester (L-NAME), indomethacin, clotrimazole, L-NAME plus indomethacin, L-NAME plus clotrimazole or tetraethylammonium (TEA). The CPP differed significantly between the female and sham-operated male animals. Gonadectomy reduced the CPP only in female rats. Differences in E2-induced relaxation were observed between the female and male animals, but male castration did not alter this response. For both sexes, the relaxation response to E2 was, at least partly, endothelium-dependent. The response to E2 was reduced only in the sham-operated female rats treated with L-NAME. However, in the presence of indomethacin, clotrimazole, L-NAME plus indomethacin or L-NAME plus clotrimazole, or TEA, the E2 response was significantly reduced in all groups. These results highlight the importance of prostacyclin, endothelium-derived hyperpolarizing factor, and potassium channels in the relaxation response of coronary arteries to E2 in all groups, whereas nitric oxide may have had an important role only in the sham-operated female group.

https://doi.org/10.1590/1414-431x20165058 1318 downloads

Histomorfometria do epitélio ruminal de cabritos 1/2 sangue Boer submetidos a dietas com tortas oriundas da produção do biodiesel
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Sanders, D.M.

; Calmon, T.C.B.S.

; Oliveira, R.L.

; Pinto, L.F.B.

; Estrela-Lima, A.

; Oliveira, R.L.

; Silva, T.M.

; Ribeiro, R.D.X.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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apr 2015, Volume 67 N. 2 Pages 433 - 440

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivou-se determinar o melhor nível de inclusão e de substituição das tortas de dendê e amendoim, respectivamente, em dietas para cabritos 1/2 sangue Boer, por meio da avaliação histomorfométrica das papilas ruminais. Foram utilizados 40 cabritos 1/2 sangue Boer, para cada período experimental, machos, não castrados, com idade aproximada de três meses e com peso inicial de 15,01±1,76kg. Os períodos experimentais constaram de 75 dias e 72 dias. As dietas consistiram de volumoso feno de Tifton-85 e de ração em mistura completa, contendo níveis de inclusão da torta de dendê nas proporções de 0,0; 7,0; 14,0; e 21,0% com base na matéria seca; e de mistura completa, contendo níveis de substituição do farelo de soja pela torta de amendoim nas proporções de 0,0; 33,0; 66,0; e 100%, constituindo-se os tratamentos. No epitélio do rúmen, foram avaliadas altura e largura das papilas, assim como densidade papilar e espessura da parede muscular do rúmen. Entre estas, a altura das papilas, no experimento com torta de amendoim, foi a única a sofrer efeito linear decrescente (P<0,0014); as demais variáveis, tanto dos animais alimentados com torta de amendoim quanto daqueles alimentados com torta de dendê, não foram afetadas pela dieta. As médias de altura encontradas foram de 2,6 e 2,3mm para as tortas de amendoim e dendê, respectivamente. Portanto, a inclusão de até 21% de torta de dendê e a substituição de 100% de torta de amendoim no concentrado de cabritos não alteraram a morfometria das papilas ruminais.

The aim of this study was to determine the best level of pies and replacement of palm oil and peanut, respectively, in diets of 1/2 blood Bôer goats from the histomorphometry of the rumen papillae. A total of 40 1/2 blood male, unneutered, aged approximately three months and initial weight of 15.01±1.76kg Bôer goats were used in each experimental period. The experimental periods consisted of 75 days and 72 days. The diets consisted of roughage hay Tifton-85 and total mixed ration containing levels of inclusion of palm kernel cake in the proportions of 0.0, 7.0, 14.0 and 21.0% based on dry matter; and the second experiment consisted of a complete mixture containing substitution levels of soybean meal by groundnut cake in the proportions of 0.0, 33.0, 66.0 and 100%. Epithelial cells were evaluated in the rumen height and width of the papillae, and density and thickness of the papillary muscle of the rumen. Among these, the height of the papillae in the experiment with peanut butter pie was the only one to suffer a negative linear effect (P<0.0014), the other variables, both in animals fed groundnut cake and palm oil, were not affected by the diet. The average heights found were 2.6 and 2.3, for pies, peanut and palm oil, respectively. Therefore, the inclusion of up to 21% palm kernel cake and replacement of 100% peanut cake in the concentrate of kids did not alter the morphology of the rumen papillae.

https://doi.org/10.1590/1678-6188 1413 downloads

Potencialidade social e econômica da pesca e maricultura no Estado do Espírito Santo, Brasil
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Teixeira, J. B.

; Lima, A.C.

; Boechat, F.P.

; Rodrigues, R.L.

; Freitas, R. R.

Revista de Gestão Costeira Integrada

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dec 2012, Volume 12 N. 4 Pages 569 - 575

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O estado do Espírito Santo (Brasil) é possui uma extensão litorânea (~ 410 km) com 15 municípios costeiros onde existe atividade pesqueira marinha. Quanto à aquicultura, ela foi introduzida na década de 1980, e desde então houve um grande desenvolvimento dessa atividade. Os primeiros passos, em 1987, foram dados de forma experimental no município de Piúma. Já nos últimos 17 anos, a atividade desenvolveu-se com fins comerciais em outros cinco municípios por meio de projetos com objetivos similares, mas com estratégias diferentes. Em face desse cenário, o presente estudo se mostrou oportuno e possibilita apontar um conjunto de informações dos aspectos econômicos e sociais da pesca e da maricultura no Estado. Essas informações podem contribuir para a definição de áreas estratégicas para o desenvolvimento das atividades de pesca e maricultura de forma sustentável, subsidiando a formulação de políticas de ordenamento do território e orientando as esferas governamentais na tomada de decisões sobre políticas públicas. Como resultado, verificou-se que, além das potencialidades já existentes, há necessidade que o poder público dos municípios e do Estado, estabeleçam políticas de incentivo permanente a maricultura e pesca, como linhas de credito compatíveis com o perfil do pescador tradicional que precisa incrementar a sua renda, tendo em vista a pesca em declínio. Além disso, o acompanhamento das atividades é fundamental para o sucesso das mesmas. Fortalecer o associativismo, identificar gargalos e propor mudanças devem ser os principais itens de pauta da parceria maricultores/pescadores e governo, propiciando o real desenvolvimento da atividade e a redução das desigualdades sociais.

Espírito Santo State (Southeast Brazil) has 15 municipalities in its coastal zone (~140km). Aquaculture was introduced in the 1980s and during this period there was a major development of the activity. Since its beginnings in 1987 activities were experimental, as in Piúma District. In the last 17 years the activity has developed into commercial projects in five other cities. Although projects have similar goals, they diverge in strategies. This study points out a set of information regarding the economic and social aspects of fisheries and aquaculture in the state. Such information can contribute to the definition of strategic areas for the development of fisheries and aquaculture activities in a sustainable manner, supporting the formulation of public policies. Our main result was to identify the need for public institutions of the state and municipalities to establish incentive permanent policies for fishing and aquaculture, with lines of credit consistent with the profile of the traditional fisherman. Moreover, monitoring the activity will be fundamental for its success. Strengthening of the association fishers-government, identification of constrains and adaptation should be the main agenda to enable the development of fisheries/aquaculture sustainability and the reduction of social inequities.

2023 downloads Cited 2 times in SciELO

10.

Comportamento ingestivo e parâmetros fisológicos de novilhos alimentados com tortas do biodiesel
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Correia, B.R.

; Oliveira, R.L.

; Jaeger, S.M.P.L.

; Bagaldo, A.R.

; Carvalho, G.G.P.

; Oliveira, G.J.C.

; Lima, F.H.S.

; Oliveira, P.A.

Archivos de Zootecnia

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mar 2012, Volume 61 N. 233 Pages 79 - 89

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Aiming to evaluate the potential of by-products coming from the production of biodiesel (palm oil, peanuts and sunflower cakes) replacing soybean meal, were evaluated the feeding behavior and bioclimatology of Holstein-Zebu steers fed diets with this byproducts. Were used four rumen canulated Holstein-Zebu steers, kept in individual pens and distributed in a 4 x 4 Latin square with repeated measures on the time. The experiment lasted 88 days and the animals were fed Tifton-85 hay and concentrate ration with soybean meal or one of the following cake: palm kernel, peanuts or sunflower, which constituted the four treatments. Ingestive behavior was assessed by observation and recording of daily time spent on eating, ruminating and idle. The bioclimatological behavior of the animals was evaluated through the following measurements: respiratory frequency (RF), heart frequency (FH) and rectal temperature (RT). The time spent for ingestion and rumination did not differ (p>0.05) between diets, however, animals fed the diet with palm kernel meal, spent more time in idle (p<0.05). There was difference for the physiological parameters only for heart rate (HR), and the diet without additional cake that provided the greatest value. The substitution of soybean meal with palm kernel meal coming from the production of biodiesel in the diet of steers affected the ingestive animal behavior.

Com o objetivo de avaliar o potencial de tortas oriundas da produção de biodiesel (dendê, amendoim e girassol) em substituição ao farelo de soja, avaliou-se o comportamento ingestivo e parâmetros fisiológicos de novilhos Holandês x Zebu submetidos a dietas contendo estes subprodutos. Foram utilizados quatro novilhos Holandês x Zebu, fistulados no rúmen, mantidos em baias individuais e dispostos em um quadrado latino 4 x 4 repetido no tempo. O experimento teve duração de 88 dias e os animais foram alimentados com feno de Tifton-85 e mistura concentrada com farelo de soja, tortas de dendê, amendoim e girassol, que constituíram os quatro tratamentos. O comportamento ingestivo foi avaliado por observação e registro dos tempos diários despendidos com alimentação, ruminação e ócio. O comportamento bioclimatológico dos animais foi avaliado por intermédio das seguintes medições: frequência respiratória (FR), frequência cardíaca (FC) e temperatura retal (TR). Não houve diferença entre as dietas para o tempo despendido tanto na ingestão, como na ruminação (p>0,05), entretanto, os animais que consumiram a dieta com torta de dendê despenderam mais tempo em ócio (p<0,05). Nos parâmetros fisológicos, ocorreu diferença apenas para frequência cardíaca (FC), sendo a dieta sem torta adicional a que proporcionou o maior valor. A substituição do farelo de soja pela torta de dendê oriunda da produção do biodiesel na dieta de novilhos afetou o comportamento ingestivo dos animais.

https://doi.org/10.4321/S0004-05922012000100009 795 downloads Cited 3 times in SciELO

11.

A new approach for data transmission system on topological surfaces
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Lima, João D.

; Silva, Francisco M.

; Villareal, Elmer R.L.

Computational & Applied Mathematics

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2012, Volume 31 N. 1 Pages 143 - 168

Abstract: En | Text: En | PDF: En

This work introduces a new data transmission system in which the main blocks, coding, modulation and channel are designed on a Riemannian manifolds. An intrinsic algebraic structure to the manifolds (surface), the homology group will be used to compose an error corrector code, a partition on the surface extracted from the embedding of a graph, which will compose the modulation design, and the channel design is the result of an association rule applied to the embedded graph. Mathematical subject classification: Primary: 06B10; Secondary: 06D05.

1972 downloads

12.

Bone regeneration in mandible defect with autograft bone and cell suspension from bone marrow in rabbits
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Gomes, C.

; Paz, A.H.

; Bonilla, A.T.

; Corrêa, A.

; Cavalcante, R.L.

; Veiga, D.

; Colomé, L.M.

; Foerstnow, L.P.

; Contesini, E.A.

; Cirne-Lima, E.O.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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aug 2011, Volume 63 N. 4 Pages 836 - 843

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Avaliou-se a regeneração óssea de auto-enxerto, considerado "padrão ouro" da crista ilíaca associado à terapia celular da medula óssea em coelhos. Foi criado um defeito ósseo de 10x5x5mm na mandíbula de 28 coelhos, distribuídos em grupo-controle com, 14 animais, reparados com auto-enxerto de crista ilíaca, e grupo experimental com, 14 animais, em que o auto-enxerto foi associado a células mononucleares da medula óssea autógena do fêmur. Foram realizadas radiografias semanais da região operada e análise histológica em sete animais de cada grupo aos 15 e em sete de cada grupo aos 30 dias do pós-operatório. Houve aumento gradativo da densidade óssea, e 85,7% (6/7) dos animais do grupo experimental e 42,8% (3/7) do grupo-controle apresentaram formação de ponte óssea 28 dias após a cirurgia. Na análise histopatológica aos 15 dias, os enxertos foram facilmente visualizados e a atividade das células fagocitárias foi intensa. Já aos 30 dias, a visualização foi mais difícil e, quando possível, apenas um resquício foi visualizado. Os resultados sugerem que a adição de células mononucleares da medula óssea favorece a regeneração do auto-enxerto em defeitos mandibulares de coelhos.

The objective of this study was to investigate the bone regeneration of a "gold standard" (autograft) from iliac crest associated with cellular therapy in rabbits. A bone defect was created with 10x5x5mm in 28 rabbit mandibles. The control group animals (n=14) were repaired with autograft of iliac crest and the experimental group animals (n=14) received iliac crest autograft in association with mononuclear cells from the bone marrow of the femur. Weekly radiographs were taken of the surgery region and histological analyses was performed in seven animals in each group at 15 days and in seven animals of each group at 30 days after the surgery. A gradual increase of bone density was observed and the experimental animals presented the bone bridge in 85.7% (6/7) of the cases, while only 42.8% (3/7) of the animals in the control group presented this structure 28 days after the surgery. The histopathological parameters analyzed did not show any statistical difference between the control and experimental group in 15 and 30 days of analysis. The results suggest that the mononuclear cells from the marrow bone can better support the autograft regeneration in mandible defects in rabbits.

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13.

Pesquisa de anticorpos anti-Brucella abortus e anti-Brucella ovis em ovinos no município de Uberlândia, MG
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Salaberry, S.R.S.

; Paulin, L.M.

; Santana, R.L.

; Castro, J.R.

; Lima-Ribeiro, A.M.C.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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aug 2011, Volume 63 N. 4 Pages 1022 - 1024

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

The first epidemiologic inquiry to Brucella abortus (B. abortus) and Brucella ovis (B. ovis) was carried out in sheep from Uberlândia county, MG. A total of 334 blood serum samples of sheep from both sexes and different ages and breeds were collected in 12 farms. An epidemiologic questionnaire was applied for each farm. Tests for B. abortus and B. ovis antibodies were Buffered Acidified Antigen and Complement Fixation, respectively. None of the sheep was reactive to B. abortus and B. ovis; however, the adoption of sanitary measures is important to avoid the introduction of infections caused by these bacteria.

3156 downloads

14.

Consumo, digestibilidade e pH ruminal de novilhos submetidos a dietas com tortas oriundas da produção do biodiesel em substituição ao farelo de soja
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Correia, B.R.

; Oliveira, R.L.

; Jaeger, S.M.P.L.

; Bagaldo, A.R.

; Carvalho, G.G.P.

; Oliveira, G.J.C.

; Lima, F.H.S.

; Oliveira, P.A.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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apr 2011, Volume 63 N. 2 Pages 356 - 363

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O experimento foi realizado com quatro novilhos Holandês x Zebu, fistulados no rúmen, mantidos em baias individuais e dispostos em um quadrado latino 4x4 repetido no tempo. Teve duração de 88 dias e os animais foram alimentados com feno de tifton-85 e mistura concentrada com farelo de soja, tortas de dendê, amendoim e girassol, que constituíram os quatro tratamentos. Avaliaram-se o consumo, a digestibilidade aparente total e o pH ruminal. O consumo de matéria seca (MS), proteína bruta (PB), carboidratos não fibrosos (CNF) e nutrientes digestíveis totais (NDT) foi menor (P<0,05) na dieta com torta de dendê em relação às demais. Os consumos de extrato etéreo (EE) e fibra em detergente neutro (FDN) não diferiram entre dietas (P>0,05). As digestibilidades totais dos nutrientes não foram influenciadas pelas dietas (P>0,05). A concentração do pH ruminal não foi influenciada pelas dietas, entretanto houve efeito do tempo de coleta. As tortas de amendoim e girassol oriundas da produção do biodiesel podem substituir o farelo de soja na dieta de novilhos. A substituição total do farelo de soja pela torta de dendê diminuiu o consumo de MS.

The experiment was conducted with four rumen canulated Holstein-Zebu steers, kept in individual pens and distributed in a 4x4 Latin square with repeated measures of time. The experiment lasted 88 days and the animals were fed Tifton-85 hay and concentrate ration with soybean meal or one of the following cakes: palm kernel, peanuts or sunflower, which constituted the four treatments. Intake, total apparent digestibility and rumen pH were evaluated. Dry matter (DM), crude protein (CP), non-fiber carbohydrates (NFC) and total digestible nutrients (TDN) intakes were lower (P<0.05) in diets containing palm kernel cake. Intake of ether extract (EE) and neutral detergent fiber (NDF) did not differ between diets (P>0.05). The total digestibility of nutrients was not affected by the diets (P>0.05). Rumen pH was not influenced by the diets; however, it was affected by time of collection. Peanut and sunflower cake originated from the production of biodiesel can replace the soybean meal in the diet for steers. Total replacement of soybean meal with palm kernel cake decreased DM intake.

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15.

ApoE polymorphisms and diarrheal outcomes in Brazilian shanty town children
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Oriá, R.B.

; Patrick, P.D.

; Oriá, M.O.B.

; Lorntz, B.

; Thompson, M.R.

; Azevedo, O.G.R.

; Lobo, R.N.B.

; Pinkerton, R.F.

; Guerrant, R.L.

; Lima, A.A.M.

Brazilian Journal of Medical and Biological Research

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mar 2010, Volume 43 N. 3 Pages 249 - 256

Abstract: En | Text: En | PDF: En

A series of studies have shown that the heavy burdens of diarrheal diseases in the first 2 formative years of life in children living in urban shanty towns have negative effects on physical and cognitive development lasting into later childhood. We have shown that APOE4 is relatively common in shanty town children living in Brazil (13.4%) and suggest that APOE4 has a protective role in cognitive development as well as weight-for-height in children with heavy burdens of diarrhea in early childhood (64/123; 52%), despite being a marker for cognitive decline with Alzheimer’s and cardiovascular diseases later in life. APOE2 frequency was higher among children with heaviest diarrhea burdens during the first 2 years of life, as detected by PCR using the restriction fragment length polymorphism method, raising the possibility that ApoE-cholesterol balance might be critical for growth and cognitive development under the stress of heavy diarrhea burdens and when an enriched fat diet is insufficient. These findings provide a potential explanation for the survival advantage in evolution of genes, which might raise cholesterol levels during heavy stress of diarrhea burdens and malnutrition early in life.

2150 downloads Cited 3 times in SciELO

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