Abstract Studies focusing on terminal drought combined with heat impacts on plants of agronomic value remain scarce, and even less under field conditions. The objective of this study was to investigate leaf structural and ultrastructural changes induced by heat stress (HS) and drought stress (DS) during seed filling and their relationship with physiological variables and yield determination. Two soybean cultivars were grown in field conditions. During seed filling four treatments were applied, including a control (without manipulation, at ambient temperature and field capacity), HS (episodes exceeding 32°C for 6 h d-1) during 21-d, DS (20% of field capacity soil water content) during 35-d, and HS×DS. Drought principally reduced leaf area, whereas heat decreased leaf thickness, possible as acclimation strategies, but also irreversible reducing CO2 assimilation sites. Both stresses damaged the outer and inner membranes of chloroplasts, causing swollen chloroplasts and accumulation of plastoglobules, loss of chlorophyll content, and negatively affecting chlorophyll fluorescence. Thus, the performance and integrity of the photosynthetic machinery were reduced. Through a morpho-functional perspective and a holistic multiscale approach, our results provide evidence of photosynthesis impairment and yield drops under stressful conditions which were associated with structural and ultrastructural (particularly at the level of chloroplasts) modifications of leaves.

Objective: Describe a case report of a hydatid splenic cyst of one year of evolution, treated by laparoscopy. Materials and Methods: A 36-year-old woman with left upper quadrant pain for 1 year. The topographic study showed a 67mm splenic cystic lesion. Laparoscopic splenectomy was performed without incident. Result: Favorable post-operative evolution. Histology compatible with splenic hydatidosis. Discussion: The incidence of splenic hydatidosis is low, even in endemic areas, approaching 1-2%. Surgery is the therapeutic strategy of choice, given the risks of rupture. However, there is no consensus regarding the technique. Conclusion: Laparoscopic splenectomy is a safe procedure supported by the literature.

Resumen Objetivo: Describir el caso clínico de un paciente con quiste esplénico hidatídico de un año de evolución resuelto por vía laparoscópica. Materiales y Métodos: Mujer de 36 años con dolor en hipocondrio izquierdo por 1 año de evolución. El estudio topográfico objetivó lesión esplénica quística de 67 mm. Se realizó esplenectomía laparoscópica sin incidentes. Resultado: Evolución posoperatoria favorable. Histología compatible con hidatidosis esplénica. Discusión: La incidencia de hidatidosis esplénica es baja, incluso en áreas endémicas, aproximándose al 1-2%. La cirugía es la estrategia terapéutica de elección dado los riesgos de ruptura, sin embargo, no hay consenso respecto a la técnica de elección. Conclusión: La esplenectomía laparoscópica es una herramienta segura y avalada en la literatura.