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au:Juliano, D. S.

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PhotoMetrix UVC: A New Smartphone-Based Device for Digital Image Colorimetric Analysis Using PLS Regression
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Costa, Adilson B. da

; Helfer, Gilson A.

; Barbosa, Jorge L. V.

; Teixeira, Iberê D.

; Santos, Roberta O.

; Santos, Ronaldo B. dos

; Voss, Mônica

; Schlessner, Sandra K.

; Barin, Juliano S.

Journal of the Brazilian Chemical Society

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mar 2021, Volume 32 N. 3 Pages 675 - 683

Abstract: En | Text: En | PDF: En

A novel free PhotoMetrix UVC is proposed for both the operation of a universal serial bus video camera (UVC) and multivariate image analysis, allowing a full solution for point-of-use analysis. A UVC was placed in an open-source 3D-printed chamber illuminated by a white light-emitting diode (LED) with controlled intensity of light. The digital images captured were converted into red, green, and blue (RGB) histograms, and regression models were used within the app. As a proof-of-concept, four adulterants in raw milk samples were determined. The coefficient of determination (R2Cal) for all models was higher than 0.99, and no significant differences (p < 0.05) between the measured and predicted values were identified. The root mean square error of calibration (RMSEC) and root mean square error of cross validation (RMSECV) were satisfactory, with values less than 0.1 and 0.7 g L-1, respectively. The recoveries ranged from 90 to 120% in spiked milk samples, and partial least square (PLS) models showed root mean square error of prediction (RMSEP) of 0.28, 0.33, 0.48 and 0.39 g L-1 for chloride, hypochlorite, hydrogen peroxide and starch, respectively. The PhotoMetrix UVC app was feasible for the colorimetric chemical analysis using a smartphone improving the applicability, mobility, and usability.

https://doi.org/10.21577/0103-5053.20200199 365 downloads

In silico characterization of microRNAs-like sequences in the genome of Paracoccidioides brasiliensis
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Curcio, Juliana S. de

; Batista, Mariana P.

; Paccez, Juliano D.

; Novaes, Evandro

; Soares, Célia Maria de Almeida

Genetics and Molecular Biology

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mar 2019, Volume 42 N. 1 Pages 95 - 107

Abstract: En | Text: En | PDF: En

Abstract Eukaryotic cells have different mechanisms of post-transcriptional regulation. Among these mechanisms, microRNAs promote regulation of targets by cleavage or degradation of the mRNA. Fungi of the Paracoccidioides complex are the etiological agents of the main systemic mycosis of Latin America. These fungi present a plasticity to adapt and survive in different conditions, and the presence of microRNAs-like molecules could be part of the mechanisms that provide such plasticity. MicroRNAs produced by the host influence the progression of this mycosis in the lungs besides regulating targets involved in apoptosis in macrophage, activation of T and B cells and the production of cytokines. Therefore, this work analyzed the presence of regions in the genome of this fungus with a potential to encode microRNAs-like molecules. Here we show by analysis of sequence similarity the presence of 18 regions, putatively coding for microRNAs-like molecules in the Paracoccidioides brasiliensis genome. We also described the conservation of dicer and argonaut proteins and the cognate transcripts induced in the yeast parasitic phase. This work represents a starting point for the analysis of the presence of those molecules in the morphological stages of the fungus and their role in fungal development.

https://doi.org/10.1590/1678-4685-gmb-2018-0014 1394 downloads

Alternative Igniters Based on Oxidant Salts for Microwave-Induced Combustion Method
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Pereira, Leticia S. F.

; Iop, Gabrielle D.

; Nascimento, Mariele S.

; Diehl, Liange O.

; Bizzi, Cezar A.

; Barin, Juliano S.

; Flores, Erico M. M.

Journal of the Brazilian Chemical Society

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mar 2016, Volume 27 N. 3 Pages 526 - 533

Abstract: En | Text: En | PDF: En

In this work, solutions of oxidant salts were evaluated as alternative to nitrate solution for the ignition step in microwave-induced combustion (MIC) method. Aqueous solutions of KClO4, K2Cr2O7, KMnO4 and K2S2O8 in several concentrations were evaluated and it was observed that the ignition was dependent on the concentration and type of igniter solution. Moreover, it was possible to observe that ignition and combustion times are not related with sample mass, which was confirmed by evaluating sample masses ranging from 100 to 500 mg of whole milk powder. Certified reference materials were digested using K2Cr2O7 as igniter solution and digests analyzed by inductively coupled plasma optical emission spectrometry (ICP OES), with good agreement to certified values. The obtained results suggest that MIC can be performed using solutions of oxidants salts. In addition, the proposed study contributed to a better undestanding and explaining the ignition with microwaves.

https://doi.org/10.5935/0103-5053.20150280 5292 downloads

Preparo do solo e emissão de CO2, temperatura e umidade do solo em área canavieira
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Iamaguti, Juliano L.

; Moitinho, Mara R.

; Teixeira, Daniel D. B.

; Bicalho, Elton da S.

; Panosso, Alan R.

; La Scala Junior, Newton

Revista Brasileira de Engenharia Agrícola e Ambiental

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may 2015, Volume 19 N. 5 Pages 497 - 504

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O preparo mecânico do solo é uma das práticas agrícolas que contribuem para o aumento da perda de carbono via emissão de CO2 do solo (FCO2). Com este trabalho objetivou-se investigar o efeito de três sistemas de preparo do solo na FCO2, temperatura e umidade do solo em área de reforma da cultura de cana-de-açúcar. A área experimental foi constituída de três parcelas, cada uma recebendo um dos preparos do solo: preparo convencional (PC), subsolagem convencional (SC) e subsolagem localizada (SL). A FCO2, temperatura e a umidade do solo foram avaliadas durante o período total de 17 dias. A FCO2 foi maior no preparo PC (0,75 g CO2 m-2 h-1). A temperatura do solo não diferiu (p > 0,05) entre as subsolagens: SL (26,2 ºC) e SC (25,9 ºC). A umidade do solo foi maior na SL (24%), seguida pela SC (21,8%) e preparo PC (18,3%). Apenas no preparo PC foi observada correlação significativa (r = –0,71; p < 0,05) entre FCO2 e a temperatura do solo. O preparo PC apresentou emissão total (2.864,3 kg CO2 ha-1), superior às emissões nas subsolagens: SC (1.970,9 kg CO2 ha-1) e SL (1.707,7 kg CO2 ha-1). A conversão do sistema de preparo PC para a SL diminuiu as emissões de CO2 do solo, reduzindo a contribuição da agricultura para o aumento dos gases de efeito estufa na atmosfera.

Soil tillage is one of the agricultural practices that may contribute to increase the loss of carbon through emission of CO2 (FCO2). The aim of this study was to investigate the effect of three soil tillage systems on FCO2, soil temperature and soil moisture in a sugarcane area under reform. The experimental area consisted of three tillage plots: conventional tillage (CT), conventional subsoiling (CS), and localized subsoiling (LS). FCO2, soil temperature and soil moisture were measured over a period of 17 days. FCO2 showed the highest value in CT (0.75 g CO2 m-2 h-1). Soil temperature presented no significant difference (p > 0.05) between LS (26.2 °C) and CS (25.9 °C). Soil moisture was higher in LS (24%), followed by CS (21.8%) and CT (18.3%). A significant correlation (r= ‒0.71; p < 0.05) between FCO2 and soil temperature was observed only in CT. The conventional tillage presented a total emission (2,864.3 kg CO2 ha-1) higher than the emissions observed in CS (1,970.9 kg CO2 ha-1) and LS (1,707.7 kg CO2 ha-1). The conversion from CT to LS decreased soil CO2 emissions, reducing the contribution of agriculture in increasing the concentration of greenhouse gases in the atmosphere.

https://doi.org/10.1590/1807-1929/agriambi.v19n5p497-504 3455 downloads

II Diretriz de Ressonância Magnética e Tomografia Computadorizada Cardiovascular da Sociedade Brasileira de Cardiologia e do Colégio Brasileiro de Radiologia
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Sara, Leonardo

; Szarf, Gilberto

; Tachibana, Adriano

; Shiozaki, Afonso Akio

; Villa, Alexandre Volney

; Oliveira, Amarino Carvalho de

; Albuquerque, Andrei Skromov de

; Rochitte, Carlos Eduardo

; Nomura, César Higa

; Azevedo, Clerio Francisco

; Jasinowodolinski, Dany

; Tassi, Eduardo Marinho

; Medeiros, Fabio de Morais

; Kay, Fernando Uliana

; Junqueira, Flávia Pegado

; Azevedo, Guilherme S. A.

; Monte, Guilherme Urpia

; Pinto, Ibraim Masciarelli Francisco

; Gottlieb, Ilan

; Andrade, Joalbo

; Lima, João A. C.

; Parga Filho, José Rodrigues

; Kelendjian, Juliana

; Fernandes, Juliano Lara

; Iquizli, Leonardo

; Correia, Luis C. L.

; Quaglia, Luiz Augusto

; Gonçalves, Luiz Flavio Galvão

; Ávila, Luiz Francisco

; Zapparoli, Marcello

; Hadlich, Marcelo

; Nacif, Marcelo Souto

; Barbosa, Márcia de Melo

; Minami, Márcio Hiroshi

; Bittencourt, Marcio Sommer

; Siqueira, Maria Helena Albernaz

; Silva, Marly Conceição

; Lopes, Marly Maria Uellendahl

; Marques, Mateus Diniz

; Vieira, Mônica La Rocca

; Coellho Filho, Otávio Rizzi

; Schvartzman, Paulo R.

; Santos, Raul D.

; Cury, Ricardo C.

; Loureiro, Ricardo

; Cury, Roberto Caldeira

; Sasdelli Neto, Roberto

; Macedo, Robson

; Cerci, Rodrigo Julio

; Faria Filho, Rui Alberto de

; Cardoso, Sávio

; Naves, Thiago

; Magalhães, Tiago Augusto

; Senra, Tiago

; Burgos, Ursula Maria Moreira Costa

; Moreira, Valéria de Melo

; Ishikawa, Walther Yoshiharu

Arquivos Brasileiros de Cardiologia

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dec 2014, Volume 103 N. 6 Pages 1 - 86

Abstract: Pt | Text: Pt | PDF: Pt

https://doi.org/10.5935/abc.2014S006 67586 downloads

Pear quality characteristics by Vis / NIR spectroscopy
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Machado, Nicácia P.

; Fachinello, José C.

; Galarça, Simone P.

; Betemps, Débora L.

; Pasa, Mateus S.

; Schmitz, Juliano D.

Anais da Academia Brasileira de Ciências

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sep 2012, Volume 84 N. 3 Pages 853 - 863

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Recentemente, técnicas não destrutivas como a espectroscopia Vis/NIR têm sido utilizadas para avaliar as características de maturação e qualidade das peras. O trabalho tem como objetivo validar as leituras por espectroscopia Vis/NIR, como forma não destrutiva de avaliar as características qualitativas em peras das cultivares Williams, Packams e Carrick produzidas em condições brasileiras. O experimento foi realizado na Universidade Federal de Pelotas, UFPel, Pelotas/RS e o instrumento utilizado para determinar a qualidade dos frutos de forma não destrutiva foi o espectrofotômetro NIR-Case (SACMI, Imola, Itália). Para a determinação de sólidos solúveis (SS) e firmeza da polpa (FP) das peras, estabeleceu se a calibração para cada cultivar em estudo a partir das avaliações obtidas pelo método não destrutivo (NIR-Case) e pelo método destrutivo e, posteriormente, testou-se o desempenho destas leituras através de regressões lineares. Os resultados foram significativos para o parâmetro sólidos solúveis obtido por espectroscopia Vis/NIR, no entanto, não se obteve resultados satisfatórios para a firmeza da polpa de peras dessas cultivares. Conclui-se que a espectroscopia Vis/NIR utilizando regressão linear possibilita fornecer estimativas seguras dos índices de qualidade para peras, especialmente para os sólidos solúveis.

Recently, non-destructive techniques such as the Vis / NIR spectroscopy have been used to evaluate the characteristics of maturation and quality of pears. The study aims to validate the readings by the Vis / NIR spectroscopy as a non-destructive way to assess the qualitative characteristics of pear cultivars 'Williams', 'Packams' and 'Carrick', produced according to Brazilian conditions. The experiment was conducted at the Pelotas Federal University, UFPel, in Pelotas / RS, and the instrument used to measure the fruit quality in a non-destructive way was the NIR- Case spectrophotometer (SACMI, Imola, Italy). To determine pears' soluble solids (SS) and pulp firmness (PF), it was established calibration equations for each variety studied, done from the evaluations obtained by a non-destructive method (NIR-Case) and a destructive method. Further on, it was tested the performance of these readings by linear regressions. The results were significant for the soluble solids parameter obtained by the Vis / NIR spectroscopy; however, it did not achieve satisfactory results for the pear pulp firmness of these cultivars. It is concluded that the Vis / NIR spectroscopy, using linear regression, allows providing reliable estimates of pears' quality levels, especially for soluble solids.

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Deposição de gotas no dossel e controle químico de Phakopsora pachyrhizi na soja
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Tormen, Nédio R.

; Silva, Felipe D. L. da

; Debortoli, Mônica P.

; Uebel, Juliano D.

; Fávera, Diego D.

; Balardin, Ricardo S.

Revista Brasileira de Engenharia Agrícola e Ambiental

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jul 2012, Volume 16 N. 7 Pages 802 - 808

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A deposição de gotas no dossel da cultura da soja com três pontas de pulverização e programas de controle químico da ferrugem asiática foram avaliados em experimento conduzido em campo, no município de Itaara, RS. Os tratamentos foram arranjados no delineamento de blocos ao acaso com quatro repetições, em esquema fatorial 2 x 3 x 3, representados por duas cultivares de soja, três pontas de pulverização e três programas de controle da ferrugem asiática. Os fungicidas foram aplicados nos estádios R1 e R4 da cultura. As variáveis analisadas foram: densidade de gotas, diâmetro mediano volumétrico (DMV), índice de área foliar (IAF), severidade da ferrugem asiática e rendimento de grãos da cultura. Elaborou-se, com base nos dados de severidade da ferrugem asiática, a área abaixo da curva de progresso da ferrugem (AACPF). As pontas de pulverização estudadas diferiram significativamente quanto à deposição de gotas nos três terços do dossel vegetal, DMV, AACPF e rendimento de grãos. O incremento médio no rendimento de grãos pela aplicação de fungicidas foi de 53,17% na cultivar Asgrow 8000 RG e 29,89% na cultivar Agiara RR. O IAF das cultivares influenciou na penetração e deposição de gotas no dossel da cultura.

Drop deposition on soybean canopy with three spray nozzles and chemical control programs of asian soybean rust were evaluated in two soybean cultivars, in a study carried out in Itaara, RS. The treatments were arranged under completely randomized block design with four replications, in a factorial design 2 x 3 x 3, utilizing two soybean cultivars, three spray nozzles and three chemical control programs. The fungicide applications were accomplished in R1 and R4 growth stages. Drop density, volumetric medium diameter (VMD), leaf area index (LAI), disease severity and grain yield were the variables measured. Based on the severity data of asian rust the area under disease progress curve (AUDPC) was calculated. The spray nozzles studied were significantly different in relation to drop deposition on soybean canopy, VMD, AUDPC and grain yield. The soybean grain yield was increased by 53.17% on Asgrow 8000 RG and 29.89% on Agiara RR cultivar, as a result of fungicide application. The LAI of cultivars influenced drop deposition and penetration into canopy.

2923 downloads

Atividade do óleo essêncial de orégano contra fungos patogênicos do arroz: crescimentos micelial em placas
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Zanandrea, I.

; Juliano, D. S.

; Andréa, B. M.

; Juliane, L.

; Veridiana, K. B.

Revista Brasileira de Farmacognosia

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2004, Volume 14 Pages 14 - 16

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo deste trabalho foi testar o efeito do óleo essencial de Origanum vulgare L. sobre o crescimento micelial de fungos patogênicos do arroz. Discos de micélio dos fungos Alternaria sp., Bipolaris oryzae, Curvularia sp., Gerlachia oryzae, Rhizoctonia solani e Sclerotinia sclerotiorum foram colocados no centro de placas de Petry com BDA e óleo em diferentes concentrações: óleo puro, 1:2, 1:4, 1:8, 1:16, 1:32 as quais foram incubadas a 22 ± 2ºC sob fotoperíodo de 12 h, utilizando-se para controle placas com Batata-Dextrose-Ágar + etanol. O crescimento foi medido assim que o micélio controle preenchesse toda a placa. Todos os fungos apresentaram redução do crescimento micelial. B. oryzae foi o mais sensível, com diferença estatística em todas as concentrações.

The purpose of this study was test the effect of the Origanum vulgare essential oil on the micelial growth of the rice pathogenic fungi. Micelium discs of the fungi Alternaria sp., Bipolaris oryzae, Curvularia sp., Gerlachia oryzae, Rhizoctonia solani and clerotinia sclerotiorum were placed in the center of Petry plates with PDA and oil in different concentrations: pure oil, 1:2, 1:4, 1:8, 1:16, 1:32 which was incubated to 22 ± 2°C under 12 h photoperiod, until the control (Potato-Dextrose-Agar + ethanol) fill all the plate. All genera showed reduction in the micelial growth. B. oryzae was the most sensible, with statistic difference in all concentrations.

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Paralisia do olhar conjugado lateral associada a oftalmoplegia internuclear unilateral em hemorragia mesencéfalo-pontina alta: relato de um caso
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Fontanari, Juliano L.

; Vaitses, Vívian D. C.

; Cruz, Carlos E. R. Nova

; Tichauer, Thomas A. T.

; Lisboa, Liana

; Souza, Emanuel S.

Arquivos de Neuro-Psiquiatria

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dec 1986, Volume 44 N. 4 Pages 383 - 390

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Os autores relataram um caso de hemorragia mesencéfalo-pontina alta direita, com oftalmoplegia extrínseca, permanecendo apenas a convergência bilateral, a abdução voluntária e automático-reflexa do olho esquerdo, e a adução deste olho na pesquisa dos reflexos vestíbulo-oculares ("one-and-a-half syndrome"). Ainda, havia dissociação voluntária-automática da motricidade das pálpebras e dos movimentos conjugados verticais e, também, hemiplegia sensitivo-motora à esquerda e asterixis.

The authors report a case of right midbrain and high pons haemorrhage with complete external ophthalmoplegy, except bilateral convergence, voluntary and automatic-reflex abduction of the left eye and adduction of this eye with vestibulo-ocular reflex ("one-and-a-half syndrome"). There is voluntary-automatic dissociation of the eyelids motricity and the vertical gaze. Still, there is sensitive-motor hemiplegia at left and asterixis.

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