The aim of this article is to describe a case of leiomyosarcoma of the mandible with immunohistochemical analysis that was useful in making the final diagnosis. A 40-year-old woman was referred to the Stomatology Clinic of São Paulo Tatuapé Hospital, , for evaluation of a lesion on the left side of the mandible. This lesion presented a fast growth in the last 6 months. Intraoral examination showed a firm, fixed, red colored mass measuring, approximately 60-mm in diameter. No lymph nodes involvement was found. The radiographic examination showed a lytic lesion showed ill-demarcated radiolucent with facial and lingual cortical bone destruction. Microscopic examination of the mandibular lesion showed a neoplasm composed by interlacing fascicles of spindle-shaped cells. Most of the cells presented a blut-ended elongated shape. A marked cellular pleomorphism was observed, represented by cells with irregular shape and abundant eosinophilic cytoplasm. Nuclei were large, hyperchromatic, either vacuoled or cigar-shape. The cytoplasm of the cells stained red with Masson’s trichrome stain. Neoplastic cells expressed vimentin, smooth-muscle actin, HHF-35 and desmin. These findings were consistent with the diagnosis of leiomyosarcoma.

O objetivo deste trabalho foi analisar morfologicamente as superfícies ósseas resultantes da secção por pontas diamantadas ou por laser de érbio:YAG. Cinco ratos (Rattus norvegicus albinus foram sacrificados por dose letal de fenobarbital. Após a execução deste procedimento, os ossos predeterminados foram submetidos à secção por pontas diamantadas ou por laser de érbio:YAG em uma energia de 300 mJ por pulso e taxa de repetição de 2 Hz. As amostras foram submetidas a análise em microscópio eletrônico de varredura, revelando a existência de um padrão para as secções obtidas com cada instrumento, sendo verificada uma superfície mais regular nas amostras seccionadas com o laser de érbio:YAG. Em aumentos da ordem de 3000 vezes, pode-se observar indícios de fusão e seqüente solidificação das superfícies seccionadas por meio do laser de érbio:YAG. Conclui-se que o laser de érbio:YAG foi eficaz na remoção de tecido ósseo, mas que, nos parâmetros utilizados neste estudo, foi responsável por alterações morfológicas sugestivas de significativo aumento de temperatura, não devendo ser indicado, nestas condições, para a execução de secções ósseas.

The purpose of this study was to analyze bone surfaces cut by Er:YAG laser or by diamond points. Five male rats (Rattus norvegicus albinus) were killed by lethal dose of phenobarbital, and selected bones were cut by Er:YAG laser at 300 mJ and 2 Hz, or by diamond points. The samples were submitted to evaluation through SEM at standard magnifications. By analyzing the surfaces, the authors could observe a pattern for each group, with a smoother surface in the laser cut group than in the diamond cut group. In higher magnifications, the images suggest melting and solidifying in the laser group. The authors conclude that the laser was effective in bone cutting. However, it was responsible for morphological changes that suggest a significant temperature increase.

No presente trabalho foi realizado estudo em microscopia eletrônica de varredura do fungo Piedraia hortae, através de material obtido de lesões no cabelo de índios do Amazonas. O exame em microscopia eletrônica revelou ascosporos poliédricos, ovóides e arredondados, arranjados isoladamente ou na forma de pseudohifas, de permeio a material extracelular densamente compactado.

Scanning electron microscopy (SEM) study of the fungus Piedraia hortae was performed from material obtained from infected hairs of Amazon indians. The SEM examination revealed ascospores poliedrical, ovoids and spherical of fungus Piedraia hortae, ar-ranjed isolated or in a pseudohyphae pattern, in a densely packed extracelular material.