Since 1984, Anopheles (Kerteszia) lepidotus has been considered a mosquito species that is involved in the transmission of malaria in Colombia, after having been incriminated as such with epidemiological evidence from a malaria outbreak in Cunday-Villarrica, Tolima. Subsequent morphological analyses of females captured in the same place and at the time of the outbreak showed that the species responsible for the transmission was not An. lepidotus, but rather Anopheles pholidotus. However, the associated morphological stages and DNA sequences of An. pholidotus from the foci of Cunday-Villarrica had not been analysed. Using samples that were caught recently from the outbreak region, the purpose of this study was to provide updated and additional information by analysing the morphology of female mosquitoes, the genitalia of male mosquitoes and fourth instar larvae of An. pholidotus, which was confirmed with DNA sequences of cytochrome oxidase I and rDNA internal transcribed spacer. A total of 1,596 adult females were collected in addition to 37 larval collections in bromeliads. Furthermore, 141 adult females, which were captured from the same area in the years 1981-1982, were analysed morphologically. Ninety-five DNA sequences were analysed for this study. Morphological and molecular analyses showed that the species present in this region corresponds to An. pholidotus. Given the absence of An. lepidotus, even in recent years, we consider that the species of mosquitoes that was previously incriminated as the malaria vector during the outbreak was indeed An. pholidotus, thus ending the controversy.
Revisão do Grupo Leucosphyrus de Anopheles (Cellia) (Diptera, Culicidae). Apresenta-se a revisão taxonômica de 20 espécies do Grupo Leucosphyrus da Série Neomyzomyia de Anopheles (Cellia). Apresentam-se as descrições morfológicas dos adultos, machos e fêmeas, genitália masculina, pupa e larva de quarto estádio, bem como dados de bionomia, distribuição e discussão sistemática para cada espécie, incluindo caracteres diagnósticos. Foram acrescentadas chaves dicotômicas de identificação para as espécies, utilizando caracteres dos adultos fêmeas e larvas de quarto estádio. Quando possível, foram incluídos dados sobre a importância médica de cada espécie. Foram também apresentadas ilustrações dos adultos, das larvas de quarto estádio e das pupas. Os mapas de distribuição das espécies foram elaborados com base no material examinado, no entanto, quando possível utilizou-se dados adicionais publicados na literatura. Fornecem-se tabelas com as variações dos caracteres dos adultos, das cerdas das larvas de quarto estádio das pupas. São designados os neótipos de An. takasagoensis Morishita e An. sulawesi Koesoemawinangoen, e o lectótipo de An. balabacensis Baisas. O nome do autor de An. sulawesi previamente citado como sendo Waktoedi foi corrigido para Koesoemawinangoen.
Revision of the Leucosphyrus Group of Anopheles (Cellia) (Diptera, Culicidae). This is a comprehensive revision of 20 species of the Leucosphyrus Group of the Neomyzomyia Series of Anopheles (Cellia). Morphological description of the adults, male and female, male genitalia, pupa and fourth-instar larva are provided for each taxon in addition to bionomics, distribution data and systematic discussion for each species, including diagnostic characters. Identification keys for females and fourth-instar larvae are provided. When possible medical importance of each species is included. Illustrations of of the adults, fourth-instar larvae and pupae are provided. Distribution maps for each species are mainly based on the material examined; however, when possible published data were also used. Tables on adult character variations, fourth-instar larval and pupal setal branching are included as appendices. A neotype for An. takasagoensis Morishita and An. sulawesi Koesoemawinangoen, and a lectotype for An. balabacensis Baisas, are designated. The authorship of An. sulawesi previously cited as Waktoedi is corrected to Koesoemawinangoen.