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DESIGN BIOINSPIRADO DE CORANTES
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Homem-de-Mello, Paula

; Souza, Ana Clara Gonzalez de

; Melo, Diêgo U.

; Mol, Gabriel S.

; Souza, Jhonathan R. De

; Carneiro, Leonardo M.

; Nobrega, Maíra G.

; Zanotto, Mateus

; Corrêa, Raissa Lohanna G. Q.

; Oliveira, Hueder P. M. de

; Bartoloni, Fernando H.

; Aoto, Yuri A.

; Coutinho-Neto, Mauricio D.

; Orestes, Ednilsom

Química Nova

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2025, Volume 48 N. 3 elocation e-20250047

Abstract: Pt | Text: Pt | PDF: Pt

The history of the discovery, use, and development of dyes is closely linked to the history of humanity itself. Among the first records of human civilization are cave paintings made from, e.g., fruits, plants, minerals, and insects. Over the centuries, using different techniques, colors have been given to all types of materials and products, from clothing to food. The chemicals responsible for colors are dyes - a vast collection of molecules of the most varied compositions, but which have in common the absorption of light within the visible window of the electromagnetic spectrum. This article aims not only to review the properties of some classes of natural dyes, their origins, and their applications but also to describe the various computational chemistry methods involved in designing new bio-inspired molecules.

https://doi.org/10.21577/0100-4042.20250047

Recurrent pulmonary thromboembolism with cardiac tamponade as initial manifestations of lupus and antiphospholipid syndrome: a case report
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Villanueva-Valle, Julieta Sofia

; Amaro-Palomo, Eder Jonathan

; Munive-Eyssautier, Mónica Andrea

; Gonzalez-Diaz, Fernando

; Sotelo-Soleno, Adrián

; Alba-Valencia, Santiago

; Arias-Mendoza, Alexandra

; Araiza-Garaygordobil, Diego

Archivos peruanos de cardiología y cirugía cardiovascular

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oct 2024, Volume 5 N. 4 Pages 237 - 242

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN El lupus eritematoso sistémico (LES) es una enfermedad autoinmune inflamatoria con un curso clínico significativo debido a su compromiso sistémico. El LES se asocia frecuentemente con el síndrome antifosfolípido y el tromboembolismo pulmonar (TEP) es particularmente común. Es extremadamente raro que el TEP sea la presentación clínica inicial y aun más inusual que coincida con un taponamiento cardíaco, lo que representa un desafío en el diagnóstico y manejo. Presentamos el caso de una mujer de 42 años con TEP recurrente, con derrame pleural y pericárdico severo, inestabilidad hemodinámica y taponamiento cardíaco. Los estudios de laboratorio revelaron hipocomplementemia, leucopenia, anticuerpos negativos para LES y anticoagulante lúpico positivo. Este caso destaca la importancia de determinar la etiología del TEP, evaluar la clasificación de riesgo e implementar el manejo adecuado, lo cual es crucial para la supervivencia y el desenlace del paciente.

ABSTRACT Systemic lupus erythematosus (SLE) is an inflammatory autoimmune disease with an important course due to systemic compromise. SLE is frequently associated with antiphospholipid syndrome, and pulmonary thromboembolism (PE) is particularly common. It is extremely rare for PE to be the initial clinical presentation and even more uncommon for it to coincide with cardiac tamponade, representing a challenge in diagnosis and management. We present a case of a 42-year-old woman with recurrent PE with severe pleural and pericardial effusion, hemodynamic instability, and cardiac tamponade. Laboratory workup revealed hypocomplementemia, leukopenia, negative SLE antibodies, and a positive lupus anticoagulant. This case emphasizes the importance of determining the etiology of PE, assessing risk classification, and implementing proper management, which are crucial for the patient's survival and outcome.

https://doi.org/10.47487/apcyccv.v5i4.418

Absence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil
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Lignon, Julia Somavilla

; Pinto, Diego Moscarelli

; Teles, Mariana Accorsi

; Trindade, Maira Aparecida Christello

; Portela, Priscila Rockenbach

; Monteiro, Silvia Gonzalez

; Martins, Kauê Rodriguez

; Cunha, Rodrigo Casquero

; Pappen, Felipe Geraldo

; Bohm, Bianca Conrad

; Bruhn, Fábio Raphael Pascoti

Revista Brasileira de Parasitologia Veterinária

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2024, Volume 33 N. 2 elocation e007924

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo As leishmanioses são doenças negligenciadas, transmitidas por vetores que acometem animais domésticos e silvestres, incluindo os humanos. Devido a sua incidência e letalidade, essa zoonose consiste em um problema de saúde pública preocupante, sendo fundamental a identificação de todos os elos da cadeia de transmissão. A infecção de mamíferos silvestres por Leishmania spp. permanece pouco compreendida, especialmente no sul do Brasil. Portanto, objetivou-se pesquisar, por meio da técnica de PCR, a presença de DNA de Leishmania spp. em mamíferos silvestres atropelados no Sul do Brasil. Carcaças de 96 animais foram coletadas, em rodovias da microrregião de Pelotas, Rio Grande do Sul, sul do Brasil e submetidas a necropsias. Fragmentos de tecidos (baço, pele, fígado, rim, coração, pulmão, linfonodos, medula óssea e sangue) foram coletados e o DNA genômico foi extraído. Protocolos de PCR visando os genes ITS1, kDNA e 18S foram testados. Não foram encontradas evidências de circulação de Leishmania spp. na população estudada. Porém, estudos epidemiológicos como este são de grande relevância, pois permitem monitorar a ocorrência de patógenos e auxiliam na identificação de possíveis áreas de risco. Como esses animais atuam como marcadores epidemiológicos da presença do microrganismo, estudos devem ser realizados continuamente, para entender se existem fontes de infecção na região.

Abstract Leishmaniasis are neglected diseases transmitted by vectors that affect domestic and wild animals, including humans. Due to its incidence and lethality, this zoonosis is a worrying public health problem, making it essential to identify all links in the transmission chain. Infection of wild mammals by Leishmania spp. remains poorly understood, especially in southern Brazil. Therefore, the objective was to research, using the PCR technique, the presence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil. Carcasses of 96 animals were collected from highways in the Pelotas microregion, Rio Grande do Sul, southern Brazil and subjected to necropsies. Tissue fragments (spleen, skin, liver, kidney, heart, lung, lymph nodes, bone marrow and blood) were collected and genomic DNA was extracted. PCR protocols targeting the ITS1, kDNA and 18S genes were tested. We found no evidence of Leishmania spp. circulation in the studied population. However, epidemiological studies like this one are of great relevance, as they allow monitoring of the occurrence of pathogens and help identify possible risk areas. As these animals act as epidemiological markers for the presence of the microorganism, studies must be carried out continuously to understand whether there are sources of infection in the region.

https://doi.org/10.1590/s1984-29612024038

ASSOCIATION BETWEEN SURGICAL VOLUME AND MORTALITY FROM COLON CANCER IN COLOMBIA: A NATIONAL COHORT
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CASTRO-CUARÁN, María Fernanda

; JUNCA, Edgar German

; GONZALEZ-PATIÑO, Diego Felipe

; BUITRAGO, Giancarlo

ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)

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2024, Volume 37 elocation e1835

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO INTRODUÇÃO: O câncer de cólon é a terceira doença maligna mais comum na Colômbia, superado apenas pelos cânceres de próstata e de mama. É o segundo câncer mais comum entre as mulheres e o terceiro mais comum entre os homens. A epidemiologia desta doença mudou na Colômbia, e o seu pico de incidência já ultrapassou a do câncer gástrico. OBJETIVOS: Determinar a associação entre o volume cirúrgico do Hospital e a mortalidade em pacientes com câncer de cólon submetidos a ressecção cirúrgica. MÉTODOS: Estudo de coorte retrospectivo nacional baseado em dados administrativos e incluiu pacientes adultos submetidos a ressecção cirúrgica para câncer de cólon que estavam inscritos no sistema de saúde contributivo entre 2012 e 2017. Definimos a exposição como o volume cirúrgico do hospital onde a cirurgia de câncer de cólon foi realizada. Classificamos os pacientes como expostos a um alto volume cirúrgico e expostos a um baixo volume cirúrgico. Os principais resultados foram a mortalidade em 30 dias e em 1 ano. Regressões de Poisson multivariadas foram utilizadas para identificar a associação entre a exposição e as taxas de mortalidade. RESULTADOS: Foram incluídos 4.647 pacientes, dos quais 4.188 pacientes foram submetidos a cirurgia em hospitais com um volume de colectomia inferior a 33 por ano e 459 foram submetidos a cirurgia em instituições com volumes iguais ou superiores a 33 por ano. Na análise multivariada, após o ajuste para variáveis observáveis, foi encontrada uma menor taxa de mortalidade em 30 dias em pacientes submetidos a cirurgia em instituições com alto volume cirúrgico. Não foram encontradas diferenças na mortalidade em um ano. CONCLUSÕES: O alto volume cirúrgico de um hospital está associado à mortalidade em 30 dias no câncer de cólon, como descrito em outros estudos, mas a mortalidade em 1 ano não mostrou essa associação. Estudos prospectivos são necessários para estabelecer uma relação causal.

ABSTRACT BACKGROUND: Colon cancer is the third most common malignancy in Colombia, only exceeded by prostate and breast cancers. It is the second most common cancer among females and the third most common among males. The epidemiology of this disease has changed in Colombia, and its peak incidence has now surpassed that of gastric cancer. AIMS: We aimed to determine the association between hospital surgical volume and mortality in patients with colon cancer undergoing surgical resection in Colombia. METHODS: This was a national retrospective cohort study based on administrative data and included adult patients undergoing surgical resection for colon cancer who were enrolled in Colombia’s contributory health system between 2012 and 2017. We defined exposure as the hospital’s surgical volume where the colon cancer surgery was performed. We classified the patients as exposed to a high surgical volume (above the 90th percentile of the provider distribution) and a low surgical volume (under the 90th percentile). The main outcomes were 30-day and 1-year mortality. Multivariate Poisson regressions were used to identify the association between exposure and mortality rates. RESULTS: The study included 4,647 patients, of which 4,188 underwent surgery at hospitals with a colectomy volume lower than 33 per year and 459 underwent surgery at institutions with volumes equal to or higher than 33 per year. In the multivariate analysis, after adjusting for observable variables, a lower risk of 30-day mortality was found in patients who underwent surgery at high surgical volume institutions (relative risk - RR 0.57, 95% confidence interval - 95%CI 0.033-0.97). No differences were found in the one-year mortality. CONCLUSION: The high surgical volume of a hospital is associated with a 30-day mortality in colon cancer, as described in other studies, but the 1-year mortality did not show this association. Prospective studies are required to establish a causal relationship.

https://doi.org/10.1590/0102-6720202400041e1835

Zoometric and ethnological characterization of the colombian creole donkey in Sucre, Colombia
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Hernández-Herrera, Darwin Yovanny

; Carrillo-Gonzalez, Diego Fernando

; Montes-Vergara, Donicer Eduardo

Revista U.D.C.A Actualidad & Divulgación Científica

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dec 2022, Volume 25 N. 2 elocation e1948

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN Es posible que el asno criollo colombiano (ACC) se encuentre en peligro de extinción. La alarmante disminución de su inventario está reduciendo la diversidad genética. Así, la caracterización morfológica es un primer paso en futuros planes de conservación, con el objetivo de repoblación; por tanto, el objetivo de esta investigación fue caracterizar zoométricamente y etnológicamente el ACC, de las cinco subregiones naturales del departamento de Sucre. En 20 machos de cada una de las subregiones naturales del departamento de Sucre (Golfo de Morrosquillo: GM, Montes de María: MM, Mojana: MO, San Jorge: SJ, Sabanas: SA), con edad promedio de 4,7 ± 1,0 años y peso promedio estimado de 126,0 ± 19,4kg, se registraron 28 medidas morfométricas. En el análisis de datos, se consideró la estadística descriptiva, el análisis de varianza entre subregiones, análisis de agrupación jerárquica. También, se estimaron cinco índices etnológicos y cinco índices productivos. La mayoría de las variables zoométricas variaron significativamente entre subregiones, con valores similares a otras razas criollas. El análisis de agrupación jerárquica formó dos grupos con cercanía geográfica. La homogeneidad estructural y la armonía morfoestructural encontrada al interior de las subregiones fue media-baja; sin embargo, al analizar todo el ACC, la homogeneidad y la armonía morfoestructural fue media-alta. Los índices etnológicos clasifican al ACC como longilíneo, hipermétrico, dolicocéfalo y concavilíneo, mientras que los índices productivos indican de un asno proporcionado, de buenos aplomos y de aptitud al trabajo.

ABSTRACT In Colombia, works performed with the colombian creole donkey (CCD) have shown a possible danger of extinction. The above, associate with an alarming decrease in its inventory and a reduction in genetic diversity. Thus, morphological characterization could be the first step for future conservation plans to conduct a repopulation. Therefore, this work aimed to characterize the zoometric and ethnological traits of CCD in five natural subregions of the department of Sucre. 20 males from each of the natural subregions from Sucre (Golfo de Morrosquillo: GM, Montes de María: MM, Mojana: MO, San Jorge: SJ, Sabanas: SA) with an age average of 4.7 ± 1.0 years old and weight estimated between 126.0 ± 19.4 kg were recorded and were submitted to 28 morphometric measurements. Descriptive statistics, analysis of variance between subregions, and hierarchical grouping for statistical analysis were performed. Also, five ethnological indices and five productive indices were estimated. The results showed that most of the zoometric variables varied significantly between subregions. The values assessed were similar to other creole donkey breeds. The hierarchical grouping analysis allowed the creation of two groups with geographic proximity. The structural homogeneity and morphostructural harmony found within the subregions were medium-low. However, when analyzing all the CCD measurements together, medium to high morphostructural uniformity and harmony were evidenced. The ethnological indices allow us to classify the CCD as a longlinear, hypermetric, dolichocephalic, and concavilinear animal. In addition, the productive indices indicated that the CCD is a proportionate donkey, with good poise and aptitude for work.

https://doi.org/10.31910/rudca.v25.n2.2022.1948

Uso de afrecho de arroz en la dieta de borregas en confinamiento
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Caballero Mascheroni, Jorge Daniel

; Gonzalez Cabañas, Javier Feliciano

; Alonzo Griffith, Luis Alberto Ocampos Olmedo, Diego Avilio Fariña Diaz, Tania Aguilar Sosa, Mauricio Alejandro

Investigación Agraria

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jun 2022, Volume 24 N. 1 Pages 1 - 7

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT The rice bran is a by-product of the rice industries that has important nutritional values and can therefore be considered as an alternative to include them in a sheep's food program. The objective of the work was to evaluate the effect of partial replacement of ground corn by rice bran on productive parameters of sheep in feedlot. The experimental design was completely randomized with four treatments and three repetitions, each repetition was composed of 2 ewes, thus totaling 12 experimental units (24 animals of 26 ± 2kg PV on average). The treatments consisted of the partial substitution of ground corn for rice bran in the diet. The levels were 0% for T1, 15% for T2, 30% for T3 and 45% for T4. The rations also contained soybean expeller, mineral salt, urea, calcium carbonate and Pangola hay (Digitaria decumbens) as a voluminous source. The duration of the experiment was 56 days. The weightings and ration adjustment were made every 14 days. The data collected were subjected to an Analysis of variance (ANOVA) at 5% error probability. The results obtained indicate that there were no significant statistical differences in the variables daily weight gain, total weight gain, intake and feed conversion ratio, with average values of 200 g.dia-1, 10.8 kg, 4.7% and 7.4 respectively. Therefore, it can be inferred that it is feasible to use rice bran instead of ground corn up to 45% without affecting the productive parameters evaluated.

RESUMEN El afrecho de arroz es un subproducto de las industrias que posee importantes valores nutricionales, por lo que puede considerarse como alternativa para incluirlas dentro de un programa alimenticio de ovinos. El objetivo del trabajo fue evaluar el efecto de la sustitución parcial del maíz molido por afrecho de arroz sobre los parámetros productivos de borregas en confinamiento. El diseño experimental fue completamente al azar con cuatro tratamientos y tres repeticiones, cada repetición estuvo compuesta por 2 borregas, totalizando así 12 unidades experimentales (24 animales de 26 ± 2kg PV y en promedio). Los tratamientos consistieron en la sustitución parcial del maíz molido por afrecho de arroz en la dieta. Los niveles fueron 0% para T1, 15% para T2, 30% para T3 y 45% para T4. Las raciones contenían además expeller de soja, sal mineral, urea, carbonato de calcio y heno de Pangola (Digitaria decumbens) como fuente de voluminoso. La duración del experimento fue de 56 días. Los pesajes y ajuste de ración fueron realizados cada 14 días. Los datos recabados fueron sometidos a un análisis de varianza (ANAVA) al 5% probabilidad de error. Los resultados obtenidos indican que no existieron diferencias estadísticas significativas en las variables ganancia diaria de peso, ganancia total de peso, consumo y conversión alimenticia, con valores promedios de 200 g.dia-1, 10,8 kg, 4,7% y 7,4 respectivamente. Por lo tanto, se puede inferir que es factible la utilización de afrecho de arroz en sustitución del maíz molido hasta un 45% sin afectar los parámetros productivos evaluados.

https://doi.org/10.18004/investig.agrar.2022.junio.2401679

Consenso peruano para el uso de la resonancia magnética en el diagnóstico y seguimiento de pacientes con esclerosis múltiple
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Caparó-Zamalloa, César Cabrejo-Bravo, José A. Castañeda-Barba, Carlos Cortez-Escalante, Jaqueline Cruz-Cruz, Ana Dueñas-Pacheco, Iván Escalante-Narrea, Diego Escalante-Rojas, Ronald Gonzalez-Mujica, Jessica Guevara-Vidal, Alembert Huamani, Charles Nisiama-Vera, Julio C. Pérez-Alviz, Carlos A. Rodríguez-Kadota, Liliana E Vega-Wong, Pamela A.

Revista de Neuro-Psiquiatría

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apr 2022, Volume 85 N. 2 Pages 95 - 106

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN El uso de la resonancia magnética (RM) en el diagnóstico y seguimiento de pacientes con esclerosis múltiple (EM) ha optimizado el cuidado de los pacientes afectados. Diversos grupos internacionales de trabajo han intentado clarificar y normatizar el uso global de la RM pero, en muchas ocasiones, se extrapolan datos de otras regiones que no contemplan la realidad de cada lugar o son difíciles de implementar. Objetivo: Consensuar aspectos relacionados con el uso de RM en el diagnóstico y seguimiento de pacientes con EM en el Perú. Material y Métodos: Un grupo de expertos peruanos, conformado por neurólogos y radiólogos, condujo la elaboración del consenso mediante metodología de ronda de encuestas a la distancia. Resultados: Las recomendaciones, basadas en la evidencia publicada y en el criterio de los expertos, enfocaron tanto el rol de las técnicas convencionales de RM como el de la medición de la atrofia cerebral en pacientes con EM al momento del diagnóstico y durante el periodo de seguimiento. Conclusiones: Las recomendaciones del consenso podrán potencialmente homogenizar y optimizar el cuidado y seguimiento de pacientes con EM en nuestro país.

SUMMARY The use of Magnetic Resonance Imaging (MRI) in the diagnosis and follow-up of patients with Multiple Sclerosis (MS) has optimized the care of the affected patients. Several international working groups have tried to clarify and standardize the global use of MRI but, on many occasions, data are extrapolated from other regions, do not contemplate local realities or are difficult to implement. Objective: To reach a consensus on aspects related to the use of MRI in the diagnosis and follow-up of patients with MS in Peru. Material and Methods: A group of Peruvian experts (neurologists and radiologists) worked on the elaboration of the consensus using a remote survey round methodology. Results: The recommendations, established on the basis of published evidence and on the experts’ criteria, focused on the role of both, the conventional MRI techniques and the measurement of brain atrophy in MS patients both at the time of diagnosis and during the follow-up period Conclusions: The consensual recommendations could potentially assist in the standardization and optimization of the care and follow-up of patients with MS in our country.

https://doi.org/10.20453/rnp.v85i2.4227

Valores éticos de la protección radiológica en procedimientos de radiodiagnóstico e intervencionismo radiológico
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Cerda, Carlos Ubeda de la Soffia-Sánchez, Pablo Inzulza-Contardo, Alonso Miranda-Gonzalez, Patricia Aragón-Caqueo, Gonzalo Aragón-Caqueo, Diego

Revista chilena de radiología

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dec 2021, Volume 27 N. 4 Pages 164 - 169

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Medical applications of ionizing radiation and in particular diagnostic and interventional radiology procedures are the main source of artificial radiation to the world population. Ionizing radiation has the potential to generate adverse health effects in people. To make safer and more efficient use of these radiations, the International Commission on Radiological Protection together with other international organizations has implemented a system of radiological protection based on three pillars: justification, optimization, and limits. In turn, these pillars are based on four fundamental ethical values: beneficence, prudence, justice, and dignity. Given that Chilean legislation does not incorporate these aspects, nor have we found national scientific articles that address these issues for diagnostic and interventional radiology procedures, the present work of narrative review aims to develop a consultation document for health professionals, which contains in a concise manner and with precise definitions, key elements to understand how the radiological protection system works and its ethical values.

Resumen Las aplicaciones médicas de las radiaciones ionizantes, y en particular los procedimientos de radiodiagnóstico e intervencionismo, son la principal fuente de radiación artificial a la población mundial. Las radiaciones ionizantes tienen el potencial de generar efectos adversos en la salud de las personas. Para hacer un uso más seguro y eficiente de estas radiaciones, la Comisión Internacional de Protección Radiológica y otras organizaciones internacionales han implementado un sistema de protección radiológica que se basa en tres pilares: justificación, optimización y límites. A su vez, estos pilares se sustentan en cuatro valores éticos fundamentales: beneficencia, prudencia, justicia y dignidad. Dado que la legislación chilena no tiene incorporados estos aspectos, ni tampoco hemos encontrado artículos científicos nacionales que aborden dichos temas para procedimientos de radiodiagnóstico e intervencionismo, el presente trabajo de revisión narrativa tiene como objetivo elaborar un documento de consulta para los profesionales de la salud que contenga de manera sucinta y con definiciones precisas los principales aspectos que hay que tener en cuenta para comprender cómo funciona el sistema de protección radiológica y sus valores éticos.

https://doi.org/10.24875/rchrad.m21000008

Evaluación del YM976 como alternativo a las hormonas gonadotrópicas en la maduración in vitro de oocitos bovinos y su competencia para el desarrollo embrionario
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Carrillo-Gonzalez, Diego Fernando Hernández Herrera, Darwin Yovanny Vásquez Araque, Neil Aldrin

Revista de Investigaciones Veterinarias del Perú

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nov 2021, Volume 32 N. 6 elocation e20341

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN El objetivo del estudio fue evaluar el efecto del YM976 como inhibidor específico de la fosfodiesterasa tipo 4 (PDE-4) sobre la maduración in vitro de oocitos bovinos y su capacidad de desarrollo embrionario. Para ello, complejos cúmulo-oocito se obtuvieron por aspiración folicular de ovarios obtenidos en planta de sacrificio. Se maduraron in vitro por 24 horas en medio TCM-199 suplementado con cuatro concentraciones de YM976 (1, 10, 100 y 1000 nM) o con gonadotropinas (LH y FSH). Se evaluó la expansión del cúmulo por observación visual y la reanudación de la metafase II por tinción con colorante Hoechst. Los oocitos fueron fertilizados y cultivados en medio KSOM Evolve por ocho días. El porcentaje de expansión grado 3 de las células de cúmulo en oocitos cultivados con gonadotropinas fue diferente (p= 0.00016) respecto de los cultivado en presencia del YM976. La reanudación de la metafase II fue similar entre oocitos madura- dos a 10 nM de YM976 y los madurados con gonadotropinas. Los oocitos madurados en concentraciones de 1 y 10 nM de YM976 presentaron un desarrollo embrionario temprano similar al encontrado en los madurados con gonadotropinas. Por el contrario, altas concentraciones de YM976 tuvieron efectos deletéreos. En conclusión, concentraciones 1 y 10 nM del inhibidor de PDE-4 YM976 durante la maduración in vitro de oocitos retrasa la expansión de las células de la granulosa, pero permite una maduración nuclear y competencia para el desarrollo embrionario, similar a los oocitos madurados con gonadotropinas, sugiriendo que, el uso de YM976 puede ser aplicado en procesos de maduración in vitro de oocitos bovinos.

ABSTRACT The aim of this study was to evaluate the effect of YM976 as a specific inhibitor of phosphodiesterase type 4 (PDE-4) on the in vitro maturation of bovine oocytes and their capacity for embryonic development. For this, cumulus-oocyte complexes were obtained by follicular aspiration of ovaries obtained in a slaughterhouse. They were matured in vitro for 24 hours in TCM-199 medium supplemented with four concentrations of YM976 (1, 10, 100 and 1000 nM) or with gonadotropins (LH and FSH). The expansion of the cumulus was evaluated by visual observation and the resumption of metaphase II by staining with Hoechst dye. The oocytes were fertilized and cultured in KSOM Evolve medium for eight days. The percentage of grade 3 expansion of the cumulus cells in oocytes cultured with gonadotropins was different (p=0.00016) compared to those cultured in the presence of YM976. The resumption of metaphase II was similar between oocytes matured at 10 nM YM976 and those matured with gonadotropins. The oocytes matured in concentrations of 1 and 10 nM of YM976 presented an early embryonic development similar to that found in those matured with gonadotropins. In contrast, high concentrations of YM976 had deleterious effects. In conclusion, concentrations of 1 and 10 nM of the PDE-4 inhibitor YM976 during in vitro maturation of oocytes delay the expansion of granulosa cells, but allow nuclear maturation and competition for embryonic development, similar to oocytes matured with gonadotropins, suggesting that the use of YM976 can be applied in in vitro maturation processes of bovine oocytes.

https://doi.org/10.15381/rivep.v32i6.20341

10.

Multiple extremity necrosis in fatal calciphylaxis: Case report
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Gonzalez, Diego Ennes

; Foresto, Renato Demarchi

; Maldonado, Ana Luiza Santos

; Padilha, Wallace Stwart Carvalho

; Roberto, Fernanda Badiani

; Pereira, Maria Eduarda Vilanova da Costa

; Durão Junior, Marcelino de Souza Carvalho, Aluizio Barbosa

Brazilian Journal of Nephrology

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jun 2021, Volume 43 N. 2 Pages 274 - 278

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Introdução: O impacto clínico da calcificação vascular está bem estabelecido no âmbito de morbimortalidade cardiovascular, mas outras síndromes clínicas, como a calcifilaxia, apesar de menos frequente, têm significante impacto na doença renal crônica. Métodos: Relato de caso de mulher, 27 anos, com queixa de dor em pododáctilos bilateralmente havia 3 dias, com presença de pequenas áreas necróticas nos locais referidos. Antecedente pessoal de diabetes tipo 1 (há 25 anos), com doença renal crônica, em diálise peritoneal, além de artrite reumatoide. Teve internação hospitalar, que antecedeu o quadro atual, devido à exacerbação da artrite reumatoide, evoluindo com trombo intracardíaco por complicação de cateter venoso, quando iniciou uso de varfarina. A isquemia progrediu para pés com necessidade de amputações bilaterais. Quirodáctilos também foram acometidos. Trombofilias, vasculites, endocardite ou outras fontes emboligênicas foram pesquisadas e descartadas. Anatomopatológico evidenciou: necrose de pele e partes moles superficiais com trombose arterial recente e calcificação medial de Monckeberg. Tratamento foi instituído com bisfosfonato e tiossulfato de sódio, conversão para hemodiálise e substituição de varfarina por heparina não fracionada. Apesar de toda a terapia, a paciente foi a óbito após quatro meses de evolução. Discussão: A calcifilaxia é uma rara síndrome de calcificação da microvasculatura que resulta em graves lesões isquêmicas. Tem patogênese relacionada ao distúrbio mineral e ósseo da doença renal crônica combinado com o desbalanço entre promotores e inibidores de calcificação vascular, com particular importância ao antagonismo da vitamina K. Conclusão: A estratégia preventiva é fundamental, uma vez que a terapia é complexa e de eficácia pouco validada.

ABSTRACT Introduction: The clinical impact of vascular calcification is well established in the context of cardiovascular morbidity and mortality, but other clinical syndromes, such as calciphylaxis, although less frequent, have a significant impact on chronic kidney disease. Methods: Case report of a 27-year-old woman, who had complained of bilateral pain in her toes for 3 days, with the presence of small necrotic areas in the referred sites. She had a history of type 1 diabetes (25 years ago), with chronic kidney disease, on peritoneal dialysis, in addition to rheumatoid arthritis. She was admitted to the hospital, which preceded the current condition, due to exacerbation of rheumatoid arthritis, evolving with intracardiac thrombus due to venous catheter complications, when she started using warfarin. Ischemia progressed to her feet, causing the need for bilateral amputations. Her chirodactyls were also affected. Thrombophilia, vasculitis, endocarditis or other embolic sources were investigated and discarded. Her pathology report evidenced skin necrosis and superficial soft parts with recent arterial thrombosis, and Monckeberg's medial calcification. We started treatment with bisphosphonate and sodium thiosulfate, conversion to hemodialysis and replacement of warfarin with unfractionated heparin. Despite all the therapy, the patient died after four months of evolution. Discussion: Calciphylaxis is a rare microvasculature calcification syndrome that results in severe ischemic injuries. It has pathogenesis related to the mineral and bone disorder of chronic kidney disease combined with the imbalance between promoters and inhibitors of vascular calcification, with particular importance to vitamin K antagonism. Conclusion: The preventive strategy is fundamental, since the therapy is complex with poorly validated effectiveness.

https://doi.org/10.1590/2175-8239-jbn-2020-0025

11.

Variabilidad de la calidad de lanas en la provincia de Río Negro, Argentina
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GONZALEZ, Ezequiel SACCHERO, Diego M. EASDALE, M.H.

RIA. Revista de investigaciones agropecuarias

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apr 2021, Volume 47 N. 1 Pages 76 - 81

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen En la provincia de Río Negro la producción de lana Merino se realiza en diversas condiciones ambientales. A pesar de esta realidad se desconoce la variabilidad de la calidad de lana a escala regional. El objetivo de este trabajo fue determinar los parámetros que mejor describan la variabilidad fenotípica de la calidad de lana de establecimientos ubicados en la provincia de Río Negro. Se utilizaron 113 establecimientos ubicados en dos regiones naturales de la provincia de Río Negro (Patagonia Occidental y Oriental). Se analizaron registros de análisis comerciales de los establecimientos (periodo 2002-2015) realizados por el Laboratorio de Fibras Textiles INTA Bariloche de lana Merino y de esquila preparto mediante técnicas de estadística multivariada (Componentes Principales). El primer plano factorial del análisis de Componentes Principales explicó el 60,66% de la variabilidad y los parámetros más influyentes en su formación fueron el RP, RT, DMF y MV. Los establecimientos de la Patagonia Oriental registraron lotes de lana de mayor DMF y MV, respecto de aquellos ubicados en la Patagonia Occidental. A su vez, la mayoría de los establecimientos de la Patagonia Oriental indicaron lotes de lana con menores porcentajes de RP y RT. La distribución de los establecimientos en el plano factorial sugeriría posibles asociaciones entre algunos parámetros de calidad de lana y la heterogeneidad del ambiente los cuales debieran estudiarse con mayor detalle a futuro.

Abstract In the province of Río Negro the production of Merino wool takes place in diverse environmental conditions. In spite of this productive reality, the variability of wool quality at regional scale is unknown. The aim was to determine the parameters that best describe the phenotypic variability of wool quality of establishments located in the province of Río Negro. We used 113 farms located in two natural regions of the province of Río Negro (Western and Eastern Patagonia). We used data from wool samples of Merino sheep of the farms (period 2002-2015) processed by the Laboratorio de Fibras Textiles from the Instituto Nacional de Tecnología Agropecuaria (INTA) Bariloche. This data set was analysed with multivariate techniques (Principal Components). The first factorial analysis of Principal Components explained 60.66% of the variability and the most influential parameters were RP, RT, DMF and MV. The farms of Eastern Patagonia registered wools with higher DMF and MV, compared to those located in Western Patagonia. At the same time, most of the farms of Eastern Patagonia indicated wools with lower percentages of RP and RT. The distribution of the farms in the factorial plane suggested possible associations between some parameters of wool quality and the heterogeneity of the environment, which should be studied with more detail in the future.

12.

Revisores 2021
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Hoffmann, Agustín Freiberg Araiza, Alejandra Bernal, Alejandro César Luna González, Alicia Isabel Migliaro Moreno, Amparo Malajovich, Ana Ramos, Ana Consuelo Lacombe, Andrea Treichel, Carlos Alberto dos Santos Valdiviano, Carlos Bacilio De La Cruz Baroni, Cecilia Cracco, Cecilia López-Steinmetz, Cecilia Marotta, Cecilia Ball, César Daniel Costa Maldonado, Daniela Patricia Ado Trías, Daniel Mola, Débora Loureto, Diego L. Hill-Denee, Diti Angeriz, Esther Ferrer, Fabiana Espíndola Rada, Fernando Novoa, Gabriela Ariza, Gladys Rocio Albistur, Gerardo Zambrano, Henry Guillén Simkin, Hugo Oliveira, Isabel C.V. de Janeiro, Isabel Janer, Jader Sánchez, Javier Dapueto, Juan Ortega, Lenise Maria Ribeiro Crespi, Leonardo Peluso Funcasta, Lorena Furlán, Luis Isidório, Marcelo dos Santos Nemes, María Ines Baptistella Ayan, María Noel Rodriguez Brenlla, María Elena Civarolo, María Mercedes Chavannes, María Ana Folle Hidalgo, María Teresa Gonzalez, María Julia Morales Palermo, Marina Míguez Verde, Mario Javier Luzardo Ferreira, Mayrla Andrade Brunet, Nicolás Núñez, Nicolás Gómez Correa, Pablo Sebastián Garzaniti, Ramiro Condeza, Rayen Rodrigues, Rogério Bustos, Romina Saldívar, Rosa María Cueto Moncada, Saul Marques, Stela Oliveira, Sueli Machado Pereira de Pribisev-Beleslin, Tamara Paz, Valentina Gradin, Victoria Beatriz Ortuño, Victor E.C.

Psicología, Conocimiento y Sociedad

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2021, Volume 11 N. 3 Pages 139 - 142

Abstract: Es | Text: Es | PDF: Es

https://doi.org/10.26864/pcs.v11.n3.rev

13.

New Health Sciences Descriptors to classify and retrieve information on equity
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Campos, Ana Cristina Espíndola Treuherz, Arthur Murasaki, Renato Toshiyuki Gonzalez, Diego Mújica, Oscar J

Revista Panamericana de Salud Pública

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2021, Volume 45 elocation e78

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN El vocabulario Descriptores en Ciencias de la Salud (DeCS) establece un lenguaje único y común que permite la organización y facilita la búsqueda y recuperación de la literatura técnica y científica en salud disponible en las fuentes de información de la Biblioteca Virtual en Salud. El DeCS, creado por el Centro Latinoamericano y del Caribe de Información en Ciencias de la Salud (BIREME), un centro especializado de la Organización Panamericana de la Salud/Organización Mundial de la Salud (OPS/OMS), es la traducción y la extensión del vocabulario Medical Subject Headings (MeSH), mantenido por la National Library of Medicine de los Estados Unidos. BIREME, en coordinación con expertos de América Latina y el Caribe, ha incluido en el DeCS los temas de equidad, género, etnicidad y derechos humanos —temas transversales en el marco programático de la cooperación técnica de la OPS/OMS— para garantizar una mejor recuperación y uso de la información y evidencia científica relacionadas a estos temas. El objetivo de este artículo es describir el método de revisión terminológica del DeCS e informar los resultados obtenidos y los impactos de la ampliación terminológica en el área de equidad, que comprendió la inclusión de 35 nuevos descriptores.

ABSTRACT The Health Sciences Descriptors (DeCS) vocabulary establishes a unique and common language that allows the organization and facilitates the search and retrieval of technical and scientific literature on health available in the information sources of the Virtual Health Library. The DeCS, created by the Latin American and Caribbean Center on Health Sciences Information (BIREME), a specialized center of the Pan American Health Organization/World Health Organization (PAHO/WHO), is the translation and extension of the Medical Subject Headings (MeSH) vocabulary, maintained by the United States National Library of Medicine. BIREME, in coordination with experts from Latin America and the Caribbean, has included in the DeCS the topics of equity, gender, ethnicity and human rights—cross-cutting themes in the programmatic framework of PAHO/WHO technical cooperation—to ensure better retrieval and use of scientific information and evidence related to these topics. The objective of this article is to describe the methodology used during the terminology review of the DeCS and to report the results obtained and the impacts of the terminology expansion in the field of equity, which included the inclusion of 35 new descriptors.

https://doi.org/10.26633/rpsp.2021.78

14.

Solitary fibrofolliculoma: a retrospective case series review over 18 years
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Díez-Montero, Cecilia

; Alonso, Miguel Diego

; Marquez, Pilar I. Gonzalez

; Schellini, Silvana Artioli Galindo-Ferreiro, Alicia

Arquivos Brasileiros de Oftalmologia

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dec 2020, Volume 83 N. 6 Pages 485 - 489

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: o objetivo deste estudo foi relatar uma série de casos de fibrofoliculoma solitário, uma lesão raramente observada nas pálpebras. Demografia, bem como aspectos clínicos e histológicos da lesão foram avaliados. Métodos: Trata-se de uma série de casos retrospectivos, com um período de 18 anos. Todos os pacientes incluídos foram diagnosticados com fibrofoliculoma solitário confirmado por exame histológico. Foram coletados dados referentes à demografia, sinais e sintomas dos pacientes, evolução da doença, localização da lesão, diagnóstico clínico e histológico e desfecho. Resultados: Onze casos de fibrofoliculoma solitário foram diagnosticados no período do estudo. A média de idade dos pacientes de 51 ± 16,3 anos (variação: 27-78 anos). A maioria dos pacientes era do sexo feminino (7/11, 64%). Cinco dos pacientes (45%) eram assintomáticos; quatro (36%) relataram sangramento, um (9%) referiu coceira e um (9%) fricção da lesão. A lesão ocorreu em luma ampla variedade de locais; um deles sendo nas pálpebras. O diagnóstico de todas as lesões foi histológico com base nos achados característicos de um folículo piloso ocasionalmente dilatado e contendo material de queratina, cercado por um manto espesso de tecido fibroso moderadamente bem circunscrito. O epitélio infundibular folicular se estendeu até esse manto fibroso, formando cordões ou cordões epiteliais. Não houve recaídas após exérese. Conclusão: Fibrofoliculoma solitário é uma lesão rara, mais ainda quando afeta as pálpebras. Relatamos 11 casos, e o terceiro relatado até o momento na literatura que afeta as pálpebras. O diagnóstico pode ser facilmente esquecido devido a sintomas inespecíficos e aparência clínica. Portanto, é necessário realizar biópsia excisional e exame histológico para o reconhecimento dessa lesão.

ABSTRACT Purpose: The purpose of this study was to report a series of cases of solitary fibrofolliculoma, a lesion seldom observed in the lids. Demographics, as well as clinical and histological aspects of the lesion were evaluated. Methods: This was a retrospective case series spanning a period of 18 years. All the included patients were diagnosed with solitary fibrofolliculoma confirmed by histological examination. Data regarding patient demographics, signs, and symptoms, course of the disease, location of the lesion, clinical and histological diagnosis, and outcome were collected. Results: Eleven cases of solitary fibrofolliculoma were diagnosed in the study period. The median age of patients was 51 ± 16.3 years (range: 27-78 years). Most patients were females (7/11; 64%). Five of the patients (45%) were asymptomatic; four (36%) reported bleeding, one (9%) had referred itching, and one (9%) rubbing of the lesion. The lesion occurred in a wide range of locations; one of them was located in the lids. The diagnosis for all lesions was histological based on characteristic findings of a hair follicle occasionally dilated and containing keratin material surrounded by a moderately well-circumscribed thick mantle of fibrous tissue. The infundibular follicular epithelium extended out into this fibrous mantle forming epithelial strands or cords. There were no relapses after exeresis. Conclusion: Solitary fibrofolliculoma is a rare lesion, seldom affecting the eyelids. We reported 11 cases, and the third case reported thus far in the literature affecting the lids. Diagnosis may be easily missed due to the nonspecific symptoms and clinical appearance. Therefore, it is necessary to perform excisional biopsy and histological examination for the recognition of this lesion.

https://doi.org/10.5935/0004-2749.20200092 332 downloads

15.

Moderator role of vitamin D concentrations on the association between metabolic syndrome and C-reactive protein among adults
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Fassula, Angelica Scherlowski

; Gonzalez-Chica, David

; Giehl, Marui Corseuil

; Silva, Diego Augusto Santos

; Cembranel, Francieli

; Moreno, Yara Maria Franco

Archives of Endocrinology and Metabolism

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dec 2020, Volume 64 N. 6 Pages 695 - 703

Abstract: En | Text: En | PDF: En

ABSTRACT Objective To evaluate the association between MetS, its components and insulin resistance (IR) with 25(OH)D and hsCRP. The moderator role of 25(OH)D in the association of MetS, its diagnostic components and IR with hsCRP were also explored. Materials and methods A cross-sectional study (2014/2015) with a population-based cohort in Southern Brazil (n = 605). Metabolic syndrome (MetS) diagnosis was defined based on the Joint Interim Statement, while the Homeostasis Model Assessment of insulin resistance (IR) (HOMA-IR) was used for determining IR. Serum concentrations of 25-hydroxy vitamin D [25(OH)D] (ng/mL) and high sensitivity C-reactive protein (hsCRP) (mg/L) were evaluated following standard protocols. 25(OH)D was categorized as sufficiency (>30 ng/mL), insufficiency (20-30 ng/mL) or deficiency (<20 ng/mL) to test its moderator role. Multiple linear regression was used to test the associations. The results were adjusted for possible confounders. Results and discussion Hypertriglyceridemia and IR were associated with lower 25(OH)D concentrations. However, except for systolic blood pressure, other MetS components and IR were associated with higher hsCRP. The association between elevated waist circumference (WC) and hsCRP was moderated by the 25(OH)D concentrations. The hsCRP median concentrations were more than two times higher among those with elevated WC and 25(OH)D insufficiency or deficiency. In this study, inadequate concentrations of 25(OH)D increased the adverse relationship between elevated WC and inflammation. 25(OH)D concentrations could be incorporated into the clinical protocols for monitoring individuals with abdominal obesity to identify those at a higher risk of complications.

https://doi.org/10.20945/2359-3997000000272 549 downloads

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