Abstract Considered a global social and public health problem that is far from over, child sexual abuse has psychological consequences for victims and their families. The objective of this research was to analyze the occurrence of symptoms of stress, anxiety and depression in mothers and to verify if such symptoms were related to the symptoms of their children who suffered sexual abuse. 65 mothers of children aged between 6 and 18 years old, recruited through social networks and support groups, participated in this research. The following instruments were applied: Child Behavior Checklist; Beck Anxiety Inventory; Beck Depression Inventory; Posttraumatic Stress Disorder Checklist and Questionnaire with sociodemographic data and characteristics of sexual abuse. Data collection was carried out online through Google Forms. The results showed severe and moderate levels for anxiety, depression and post-traumatic stress in 70.8% of the sample of participants. Multiple linear regression analyzes were performed, demonstrating that sociodemographic variables, intrafamilial sexual abuse, mother having suffered sexual abuse and the CBCL variables were not predictors of anxiety and depression symptoms in mothers. For the mothers' post-traumatic stress symptoms, it was observed that the somatic complaints of the children were predictors, explaining 70.8% of the outcome. It is concluded that most of the symptoms presented by children who suffered sexual abuse are not related to the symptoms of the mothers, showing that the occurrence of sexual abuse in itself is a factor of illness for mothers.

Resumo Considerado um problema social e de saúde pública mundial que está longe de acabar, o abuso sexual infantil tem consequências psicológicas para as vítimas e para seus familiares. O objetivo desta pesquisa foi analisar a ocorrência de sintomas de estresse, ansiedade e depressão em mães e verificar se tais sintomas estavam relacionados aos sintomas de seus filhos que sofreram abuso sexual. Participaram desta pesquisa 65 mães de filhos(as) com idades entre 6 e 18 anos, recrutadas por meio de redes sociais e grupos de apoio. Foram aplicados os seguintes instrumentos: Child Behavior Checklist; Inventário de Ansiedade de Beck; Inventário de Depressão de Beck; Posttraumatic Stress Disorder Checklist e Questionário com dados sociodemográficos e características do abuso sexual. A coleta de dados foi realizada de forma online por meio do Google Forms. Os resultados apresentaram níveis grave e moderado para ansiedade, depressão e estresse pós-traumático em 70,8% da amostra de participantes. Foram realizadas análises de regressão linear múltipla, demonstrando que as variáveis sociodemográficas, o abuso sexual intrafamiliar, a mãe ter sofrido abuso sexual e as variáveis do CBCL não foram preditores para sintomas de ansiedade e depressão das mães. Para os sintomas de estresse pós-traumático das mães, observou-se que as queixas somáticas dos filhos foram preditoras, explicando 70,8% do desfecho. Conclui-se que a maioria dos sintomas apresentados pelos filhos que sofreram abuso sexual não está relacionada com os sintomas das mães, evidenciando que a ocorrência do abuso sexual por si só é um fator de adoecimento para as mães.

Abstract Objective: to synthesize the care provided by health professionals, at different care levels, to the families of children with Autism Spectrum Disorders. Method: a qualitative study, based on the Family-Centered Care philosophical theoretical framework and developed with 22 professionals from three multidisciplinary teams from the Health Care Network of a municipality in the state of Mato Grosso do Sul, Brazil. The data were collected through two focus groups with each team, organized with the support of the Atlas.ti 8 Qualitative Data Analysis® software and submitted to Thematic Content Analysis. Results: the findings show actions centered on specific situations, especially on the demands and needs arising from the child’s care and atypical behavior. Influencing factors for family care, such as work overload and little professional experience, show the weakness of multiprofessional care and the invisibility of the family as a care unit. Conclusion: the need is highlighted to review functioning of the network for the multiprofessional care of children and their families and how such network is organized. It is recommended to offer permanent education actions that contribute to the qualification of multiprofessional teams in the care of families of children in the autism spectrum.

Resumen Objetivo: resumir la atención que brindan los profesionales de la salud, en los diferentes niveles de atención, a las familias de niños con Trastornos del Espectro Autista. Método: estudio cualitativo, basado en el marco teórico filosófico de la Atención Centrada en la Familia, desarrollado con 22 profesionales de tres equipos multidisciplinarios de servicios de la Red de Atención de la Salud en un municipio del estado de Mato Grosso do Sul, Brasil. Los datos fueron recolectados a través de dos grupos focales con cada equipo y organizados con la ayuda del software Atlas.ti 8 Qualitative Data Analysis ® y sometidos al Análisis de Contenido Temático. Resultados: los hallazgos muestran acciones enfocadas en situaciones específicas, principalmente en las demandas y necesidades que genera el cuidado del niño y su comportamiento atípico. Factores que influyen en la atención a la familia, como la sobrecarga de trabajo y la poca experiencia profesional, revelan las falencias que tiene la asistencia multidisciplinaria y la invisibilidad de la familia como unidad de atención. Conclusión: es necesario examinar el funcionamiento y la forma en que está organizada la red para la atención multidisciplinaria de los niños y sus familias. Se recomienda implementar acciones de educación continua que contribuyan a la capacitación de los equipos multidisciplinarios para la atención a las familias de niños con espectro autista.

Resumo Objetivo: sintetizar o cuidado prestado por profissionais de saúde, nos diferentes níveis de atenção, às famílias de crianças com Transtornos do Espectro Autista. Método: estudo qualitativo, baseado no referencial teórico filosófico do Cuidado Centrado na Família, desenvolvido com 22 profissionais de três equipes multidisciplinares de serviços da Rede de Atenção à Saúde de um município do estado de Mato Grosso do Sul, Brasil. Os dados foram coletados por meio de dois grupos focais com cada equipe, organizados com apoio do software Atlas.ti 8 Qualitative Data Analysis e submetidos à Análise Temática de Conteúdo. Resultados: as descobertas mostram ações centradas em situações pontuais, principalmente nas demandas e necessidades advindas do cuidado da criança e de seu comportamento atípico. Fatores influenciadores para o cuidado à família, como a sobrecarga de trabalho e a pouca experiência profissional, evidenciam a fragilidade da assistência multiprofissional e a invisibilidade da família enquanto unidade de cuidado. Conclusão: destaca-se a necessidade de rever o funcionamento e modo como a rede para o cuidado multiprofissional da criança e sua família está organizada. Recomenda-se a oferta de ações de educação permanente que contribuam com a qualificação das equipes multiprofissionais no cuidado às famílias de crianças no espectro do autismo.

ABSTRACT Understorey herbs form a diverse and understudied plant assemblage in tropical forests. Although several studies and research teams have long been dedicated to the study of this conspicuous vegetation component in Amazonia, no effort to unify the data has been undertaken to date. In contrast to trees and other life forms for which major data compilations already exist, a unified database dedicated to herbs is still lacking. Part of the problem is in defining what is a herb and how to effectively sample herb assemblages. In this article, we describe the database HERBase, an exhaustive compilation of published and unpublished data on herb inventories in Amazonia. We also describe the structure, functioning, and guidelines for data curation and integration in HERBase. We were able to compile information from 1381 plots from all six Amazonian geographic regions. Based on this dataset, we describe and discuss sampling and knowledge gaps, priority areas for new collections, and recommend sampling protocols to facilitate data integration in the future. This novel database provides a unique biodiversity data repository on understorey herbs that will enable new studies on community ecology and biogeography.

RESUMO As ervas do sub-bosque formam um componente diversificado e pouco estudado em florestas tropicais. Embora vários estudos e grupos de pesquisa tenham se dedicado ao estudo desse componente conspícuo na Amazônia, nenhum esforço foi feito até o momento para unificar essas informações. Em contraste com árvores e outros grupos de plantas para os quais já existem grandes compilações de dados, uma base de dados unificada dedicada às ervas ainda não existe. Parte do problema está em definir o que é uma erva e como amostrar comunidados de ervas de forma eficiente. Neste artigo descrevemos a base de dados HERBase, uma compilação exaustiva de dados publicados e não publicados sobre inventários de ervas na Amazônia. Também descrevemos a estrutura, funcionamento e diretrizes para curadoria e integração de dados na HERBase. Conseguimos compilar informações de 1381 parcelas de todas as seis regiões geográficas amazônicas. Com base nesses dados, descrevemos e discutimos lacunas de amostragem e conhecimento, apontamos áreas prioritárias para novas coletas e recomendamos protocolos de amostragem para facilitar a integração de dados no futuro. Essa nova base de dados fornece dados únicos de biodiversidade sobre ervas do sub-bosque que permitirão novos estudos sobre ecologia e biogeografia de comunidades.

Abstract Objective The aim of this study was to analyze the effects of fluid overload related to mechanical ventilation, renal replacement therapy, and evolution to discharge or death in critically ill children. Methods A retrospective study in a Pediatric Intensive Care Unit for two years. Patients who required invasive ventilatory support and vasopressor and/or inotropic medications were considered critically ill. Results 70 patients were included. The mean age was 6.8 ± 6 years. There was a tolerable increase in fluid overload during hospitalization, with a median of 2.45% on the first day, 5.10% on the third day, and 8.39% on the tenth day. The median fluid overload on the third day among those patients in pressure support ventilation mode was 4.80% while the median of those who remained on controlled ventilation was 8.45% (p = 0.039). Statistical significance was observed in the correlations between fluid overload measurements on the first, third, and tenth days of hospitalization and the beginning of renal replacement therapy (p = 0.049) and between renal replacement therapy and death (p = 0.01). The median fluid overload was 7.50% in patients who died versus 4.90% in those who did not die on the third day of hospitalization (p = 0.064). There was no statistically significant association between death and the variables sex or age. Conclusions The fluid overload on the third day of hospitalization proved to be a determinant for the clinical outcomes of weaning from mechanical ventilation, initiation of renal replacement therapy, discharge from the intensive care unit, or death among these children.

Resumo O objetivo foi investigar a prevalência da síndrome de burnout (SB) em profissionais de nível superior atuantes na saúde indígena no Brasil e fatores associados. Trata-se de um estudo observacional, transversal e analítico. O questionário foi aplicado (perfil e MBI-HSS) a 513 profissionais. A presença da SB foi identificada em 65% dos profissionais. Foi verificada maior chance de exaustão emocional entre os profissionais mais novos, com mais tempo de atuação na saúde indígena, em função assistencial e com menor nível de tranquilidade ao trabalhar durante a pandemia. Mais chance de apresentar baixa realização pessoal no trabalho foi observada entre os profissionais mais velhos, com menos tempo de atuação na saúde indígena, que estavam em atendimento clínico durante a pandemia e que relataram menor nível de tranquilidade ao trabalhar durante a pandemia. Maior chance de despersonificação em profissionais casados, em atendimento clínico durante a pandemia e com menor nível de tranquilidade ao trabalhar durante a pandemia. O estudo contribui com uma importante avaliação da existência de preditores da SB nos profissionais atuantes na saúde indígena.

Abstract The aim of this study was to investigate the prevalence of burnout syndrome in higher education health professionals working in indigenous health in Brazil, and to identify associated factors. This is an observational, analytical, and cross-sectional study. Data collection was based on the application of a questionnaire (personal profile and MBI-HSS) and included 513 professionals. The prevalence of burnout was 65%. Greater likelihood of emotional exhaustion was observed among younger professionals, who had worked in indigenous health for longer time, in the care function and with lower level of tranquility when working during the pandemic. Lower likelihood of having low personal accomplishment at work was observed among older professionals, professionals who had worked in indigenous health for shorter time, who worked in clinical care during the pandemic and those with lower level of tranquility when working during the pandemic. Greater likelihood of depersonalization was found among married professionals, who worked in clinical care during the pandemic and those with lower level of tranquility when working during the pandemic. This study contributes with an important assessment of the existence of BS predictors in health professionals working in indigenous health.

Resumo O objetivo foi investigar a prevalência da síndrome de burnout (SB) em profissionais de nível superior atuantes na saúde indígena no Brasil e fatores associados. Trata-se de um estudo observacional, transversal e analítico. O questionário foi aplicado (perfil e MBI-HSS) a 513 profissionais. A presença da SB foi identificada em 65% dos profissionais. Foi verificada maior chance de exaustão emocional entre os profissionais mais novos, com mais tempo de atuação na saúde indígena, em função assistencial e com menor nível de tranquilidade ao trabalhar durante a pandemia. Mais chance de apresentar baixa realização pessoal no trabalho foi observada entre os profissionais mais velhos, com menos tempo de atuação na saúde indígena, que estavam em atendimento clínico durante a pandemia e que relataram menor nível de tranquilidade ao trabalhar durante a pandemia. Maior chance de despersonificação em profissionais casados, em atendimento clínico durante a pandemia e com menor nível de tranquilidade ao trabalhar durante a pandemia. O estudo contribui com uma importante avaliação da existência de preditores da SB nos profissionais atuantes na saúde indígena.

Abstract The aim of this study was to investigate the prevalence of burnout syndrome in higher education health professionals working in indigenous health in Brazil, and to identify associated factors. This is an observational, analytical, and cross-sectional study. Data collection was based on the application of a questionnaire (personal profile and MBI-HSS) and included 513 professionals. The prevalence of burnout was 65%. Greater likelihood of emotional exhaustion was observed among younger professionals, who had worked in indigenous health for longer time, in the care function and with lower level of tranquility when working during the pandemic. Lower likelihood of having low personal accomplishment at work was observed among older professionals, professionals who had worked in indigenous health for shorter time, who worked in clinical care during the pandemic and those with lower level of tranquility when working during the pandemic. Greater likelihood of depersonalization was found among married professionals, who worked in clinical care during the pandemic and those with lower level of tranquility when working during the pandemic. This study contributes with an important assessment of the existence of BS predictors in health professionals working in indigenous health.

Abstract Chemical and pharmacological potentials of botanical species found in the Cerrado are well-known and widely studied. Chemical diversity of secondary metabolites produced by plants that belong to this Brazilian biome has triggered several studies in the fields of farming, pharmaceutical and cosmetic industries. Therefore, this study aimed at evaluating the chemical composition of essential oils (EOs) from fresh leaves of three species found in the Brazilian Cerrado: Cardiopetalum calophyllum Schltdl. (EO-CC), Campomanesia adamantium (Cambess.) O. Berg (EO-CA) and Protium ovatum Engl. (EO-PO) and at determining their anti-Malassezia furfur and anti-inflammatory activities. Both GC-FID and GC-MS showed that the most abundant chemical class of the oils was the one of sesquiterpene hydrocarbons. The major constituents identified in EO-CC were germacrene D (34.9%) and bicyclogermacrene (26.8%). EO-CA exhibited mainly germacrene D (21.7%) while EO-PO had high concentrations of germacrene D (25.0%) and γ-muurolene (18.6%). EOs were tested by the broth microdilution method on 96-well microplates and exhibited satisfactory activity against M. furfur, i. e., EO-CA had MIC = 375 µg/mL while EO-CC and EO-PO had MIC = 750 µg/mL. The chemotaxis model, which was used for evaluating their anti-inflammatory activity, showed that EOs exhibited effective results: C. calophyllum (EO-CC; IC50 = 24.4 µg/mL), C. adamantium (EO-CA; IC50 = 15.7 µg/mL) and P. ovatum (EO-PO; IC50 = 32.5 µg/mL). In short, biological activities of EO-CC, EO-CA and EO-PO, such as anti-Malassezia furfur and anti-inflammatory ones, were investigated and described for the first time.

ABSTRACT. Curves of crop growth and nutrient extraction and export are essential to develop fertilization strategies and management plans to maximize yield and reduce environmental impacts. Our study aimed to evaluate dry matter production and both extraction and export of macronutrients by arrowroots (Maranta arundinacea L.) to be used in further crop fertilization plans. To this purpose, two experiments were carried out in field conditions using the varieties Viçosa and Seta. The experimental design was randomized with four replications and nine periods of analysis, which were defined as days after planting (105, 135, 165, 195, 225, 255, 285, 315, and 345 DAP). Dry matter, extraction, and export of macronutrients by arrowroot seed-rhizomes, aerial parts, roots, and storage roots (rhizomes) were determined. The exports of N, P, K, Ca, Mg, and S of storage rhizomes of the variety Viçosa were 104.6, 51.83, 412.24, 15.85, 36.14, and 64.36 kg ha-1, while those of the seta variety were 160.6, 71.62, 521.31, 17.57, 60.09, and 160.41 kg ha-1, respectively. Both varieties proved to be efficient soil macronutrient extractors, mainly for K. The variety Seta had higher shoot, root, seed-rhizome dry matter contents, as well as greater macronutrient extractions and exports.

ABSTRACT: Beef cattle ranching is the main economic activity in the Brazilian Pantanal, undertaken in an extensive manner, mostly in natural environments and dynamic in space and time. The ongoing monitoring, assessment, and performance improvement are key aspects of the search for sustainable use of natural resources in beef cattle ranches, as these ranches are the management units in the Pantanal. In this paper we proposed a data envelopment analysis (DEA) approach to assess the performance of fourteen beef cattle ranches in the Pantanal wetland as a case study, considering financial, productive, and environmental sustainability dimensions. We modeled each dimension separately and calculated an overall performance score. We also fit fractional regression models to study the influence of potential covariates on the performance scores. The methodological approach proposed here proved to be adequate for the case study, as the results are in line with the expectations of decision-makers regarding the performance of the ranches. The overall performance analysis was influenced by the proportion of higher quality native forage resources, which depends on the dynamics and on the spatial-temporal variation of the landscapes. Therefore, overall performance requires adaptive management of ranches based on diversity conservation, which benefits the multifunctionality of products and services.

RESUMO: A pecuária de corte é a principal atividade econômica do Pantanal brasileiro, praticada de forma extensiva em ambientes predominantemente naturais e espaço-temporalmente dinâmicos. Monitorar, avaliar e aprimorar continuamente o desempenho das fazendas de pecuária é um aspecto chave na busca do uso sustentável de recursos naturais, já que as fazendas são a unidade de manejo no Pantanal. Nesse sentido, objetivou-se neste artigo a adoção de modelos de análise envoltória de dados (DEA) para avaliar o desempenho de 14 fazendas pantaneiras como estudo de caso, considerando as dimensões da sustentabilidade financeira, produtiva e ambiental. Cada dimensão foi modelada separadamente e, na sequência, foi calculado um escore único de desempenho global. Foram ainda ajustados modelos de regressão fracionária para estudar a influência de potenciais covariáveis nos escores de desempenho. A abordagem metodológica aqui proposta mostrou-se adequada ao caso de estudo em questão, com resultados alinhados às expectativas dos tomadores de decisão quanto ao desempenho das fazendas. A análise do desempenho global foi influenciada pela proporção de recursos forrageiros nativos de melhor qualidade, que depende da dinâmica e variação espaço-temporal das paisagens. Portanto, o desempenho global requer uma gestão adaptativa das propriedades com base na conservação da diversidade, o que beneficia a multifuncionalidade de produtos e serviços.

Abstract: Introduction: The National Curriculum Guidelines (DCN, Diretrizes Curriculares Nacionais) for the undergraduate medical course emphasize the educational training in the context of primary health care and have as axes of the curricular development, the health needs of the population and the teaching, service, and community integration (TSCI), preferably in services of the Brazilian Health System (SUS). Thus, the primary health network is a unique learning scenario, as it provides students the opportunity to experience health policies, as well as the multi-professional work, in addition to the possibility of dealing with real-life problems, associating medical-academic training to the social health needs. Objectives: This work aims to analyze the characteristics of the teaching-service integration, which contribute to the university, health services and the community. It also aims to analyze the difficulties and challenges through the perception of the students, the users and the health professionals involved in the outpatient Surgery Clinic and in the extension project of UFSJ (Universidade Federal de São João del-Rei) in the health units in Tiradentes-MG. Methods: This work is a qualitative study which used, as data collection instruments, semi-structured interviews with users, students and health professionals, about the perception of Teaching Service-Community Integration (TSCI). The Content Analysis technique was used for the data analysis. Results and Discussion: The data showed that the TSCI allowed improving the quality of work in the health service, qualifying the professionals who were working there, in addition to enhancing personal achievement of the actors in this interaction. It also brought improvements to primary care, allowing an understanding of the organization of the work practice and greater effectiveness of the Basic Health Unit. Furthermore, it was possible to perceive the challenges and impasses of this integration to be overcome, such as: lack of structure and materials, a minor discomfort, but no less important, of patients to be cared by students and confrontation of the routine of local health professionals with the University. Conclusion: The university inserted in the social reality and in the primary care services trains professionals who are more qualified for more prevalent problems while benefiting the population and the local health teams.

Resumo: Introdução: As Diretrizes Curriculares Nacionais (DCN) da graduação em Medicina enfatizam a formação no contexto da atenção básica de saúde e têm como eixos do desenvolvimento curricular as necessidades de saúde da população e a integração ensino-serviço-comunidade (Iesc), preferencialmente nos serviços do Sistema Único de Saúde (SUS). Dessa forma, a rede básica de saúde é um cenário de aprendizagem singular, pois proporciona aos alunos a possibilidade de eles vivenciarem as políticas de saúde e o trabalho multiprofissional, além de permitir que lidem com problemas reais, vinculando a formação médico-acadêmica às necessidades sociais da saúde. Objetivo: Este estudo teve como objetivo analisar as características da integração ensino-serviço que contribuem para a universidade, os serviços de saúde e a comunidade, e as suas dificuldades e os seus desafios por meio da percepção dos discentes, usuários e profissionais de saúde envolvidos no ambulatório de cirurgia ambulatorial e no projeto de extensão da Universidade Federal de São João del-Rei (UFSJ) nas unidades de saúde em Tiradentes, em Minas Gerais. Método: Trata-se de estudo qualitativo que utilizou como instrumentos de coleta de dados entrevistas semiestruturadas com usuários, discentes e profissionais de saúde sobre a percepção da IESC. Para a análise dos dados, empreendeu-se a técnica de análise de conteúdo. Resultado: Os dados mostraram que a IESC permitiu melhorar a qualidade do trabalho no serviço de saúde, qualificar os profissionais ali presentes, além de ampliar a realização pessoal dos atores dessa interação. Também trouxe melhorias à atenção primária, possibilitando a compreensão da organização da prática no trabalho e maior resolubilidade da unidade básica de saúde. Ainda foi possível uma percepção dos desafios e impasses dessa integração a serem superados, como falta de estrutura e materiais, desconforto minoritário, mas, não menos importante, de pacientes a serem atendidos pelos estudantes e confronto da rotina dos profissionais de saúde locais com a universidade. Conclusão: A universidade inserida dentro da realidade social e dos serviços de atenção primária forma profissionais mais capacitados para problemas mais prevalentes ao mesmo tempo que beneficia a população e as equipes de saúde locais.

Abstract Objective: To compare high and low-fidelity simulations for the recognition of respiratory distress and failure in urgency and emergency pediatric scenarios. Methods: 70 fourth-year medical students were randomly distributed in high and low-fidelity groups and simulated different types of respiratory problems. Theory tests, performance checklists, and satisfaction and self-confidence questionnaires were used in the assessment. Face-to-face simulation and memory retention was applied. The statistics were evaluated by averages and quartiles, Kappa, and generalized estimating equations. The p-value was considered 0.05. Results: In the theory test there was an increase in scores in both methodologies (p < 0.001 ); in memory retention (p = 0.043) and at the end of the process the high-fidelity group had better results. The performance in the practical checklists was better after the second simulation (p > 0,05). The high-fidelity group felt more challenged in both phases (p = 0.042; p = 0.018) and showed greater self-confidence to recognize changes in clinical conditions and in memory retention (p = 0.050). The same group, in relation to the hypothetical real patient to be treated in the future, felt better confident to recognize respiratory distress and failure (p = 0.008; p = 0.004), and better prepared to make a systematic clinical evaluation of the patient in memory retention (p = 0.016). Conclusion: The two levels of simulations enhance diagnostic skills. High fidelity improves knowledge, leads the student to feel more challenged and more self-confident in recognizing the severity of the clinical case, including memory retention, and showed benefits regarding self-confidence in recognizing respiratory distress and failure in pediatric cases.

Novel civil construction materials based on waste can be an optimal alternative for waste management, reducing its improper disposal while engaging an otherwise discarded material as a raw material in other production processes. This research’s main objective was to develop and characterize two novel artificial stones based on waste glass from bottles and aggregated with epoxy resin (ASG-EP) and polyurethane resin (ASG-PU) from castor oil, respectively, as well as the comparison of the two developed materials. Plates were manufactured with 15% of epoxy or 15% polyurethane resins and 85% of waste glass from bottles. The mixture was molded with a process of 6Hz vibration, 600mmHg of vacuum, and hot compaction at 90ºC for EP and 80ºC for PU. Results evidenced that the development of ASGs based on waste glass from bottles is technically and economically viable.

Abstract We evaluated the genetic diversity, coancestry and heritability of an E. benthamii trial. The 115 individuals were genotyped (13 SSR) and had their height and diameter at breast height (dbh) measured. Heritability was estimated using the RR-BLUP and the pairwise kinship coefficient method. An average of nine alleles per locus was observed. The expected heterozygosity (0.655) was similar to the observed heterozygosity, with the estimated inbreeding (0.02) being low. The group coancestry (0.051) demonstrates that the trees are related to some degree. The trees were clustered in five groups using the Evanno’s method. The average kinship within each group ranges from 0.042 to 0.082. The heritability estimated by RR-BLUP was low. The heritability estimated using the kinship coefficients is moderate, reaching estimated genetic gains of 14% for dbh. After knowing how genetic groups are distributed within the population, strategies for collecting, conserving, and using these germplasm resources can be performed.

ABSTRACT Objectives: to analyze the knowledge and experience of undergraduate nursing students regarding infusion therapy and peripheral vascular access. Methods: descriptive, cross-sectional, analytical study with 123 undergraduate nursing students who answered a semi-structured instrument. Results: the majority were women, with a median age of 51 years old; 87% considered the teaching received insufficient to perform in clinical practice. The mean overall knowledge score was 78.1 (SD± 8.97). The themes catheter flushing and lock (38.1%), catheter selection (34.2%), infusion equipment (30.9%) and insertion site (30.9%) presented a significant number of errors. Conclusions: practical classes and execution of procedures in health services were predictors for a better knowledge about infusion therapy and vascular access by undergraduate nursing students.

RESUMEN Objetivos: analizar el conocimiento y experiencia de estudiantes avanzados de enfermería sobre terapia infusional y accesos vasculares periféricos. Métodos: investigación descriptiva, transversal, analítica; con 123 estudiantes del último curso de enfermería, que respondieron a un instrumento semiestructurado. Resultados: mayoría de mujeres, mediana de 51 años; el 87% considera insuficiente la enseñanza recibida para desempeñarse en la práctica clínica. El promedio general de puntos de conocimiento fue del 78% (DE± 8,97). Los temas flushing y lock del catéter (38,1%), selección del catéter (34,2%), equipos de infusión (30,9%) y punto de inserción (30,9) mostraron un número significativo de errores. Conclusiones: clases prácticas y realización de procedimientos en servicios de salud fueron el camino a un mayor conocimiento en terapia infusional y acceso vascular para los estudiantes del último curso de enfermería.

RESUMO Objetivos: analisar o conhecimento e a experiência de graduandos de enfermagem relacionados à terapia infusional e aos acessos vasculares periféricos. Métodos: pesquisa descritiva, transversal, analítica, com 123 estudantes do curso de graduação em enfermagem que responderam a um instrumento semiestruturado. Resultados: a maioria, mulheres, com mediana de 51 anos; 87% consideraram o ensino recebido insuficiente para atuar na prática clínica. A média de pontos geral de conhecimento foi de 78,1 (DP± 8,97). Os temas flushing e lock do cateter (38,1%), seleção do cateter (34,2%), equipamentos para infusão (30,9%) e sítio de inserção (30,9%) apresentaram número significativo de erros. Conclusões: aulas práticas e realização de procedimentos nos serviços de saúde foram preditores para um melhor conhecimento sobre terapia infusional e acesso vascular pelos estudantes do curso de graduação em enfermagem.

ABSTRACT Campomanesia rufa (O. Berg) Nied. is a Myrtaceae species native to the Brazilian Cerrado. As C. rufa is considered endangered, in vitro propagation is an alternative for its conservation. However, the lack of effective disinfection protocols for endophytic microorganisms naturally present in plant tissues hinders the success of micropropagation. In this context, the objective of this study was to isolate, molecular identify, and control endogenous occurring bacteria of C. rufa propagated in vitro. Purified PCR products of bacterial isolates were sequenced by the Sanger method and aligned with homologous sequences using the Basic Local Alignment Search Tool (BLAST) available in National Centre for Biotechnology Information (NCBI) database. MEGA7 software was used to align all sequences and to draw phylogenetic trees. Survival, shoot height, and efficiency of antibiotics (streptomycin, ampicillin, and chloramphenicol) at different concentrations in the culture medium were evaluated. Different Bacillus sp. strains were observed in the plant tissues. When testing the control of Bacillus sp. with antibiotics, 32 mg L-1 ampicillin caused a significant reduction in bacterial contamination with no effect on explant survival. On the other hand, 256 mg L-1 streptomycin caused the greatest reduction in contamination but was lethal to over 90% of the explants. This study is the first report on the occurrence of endogenous bacteria and their control in the context of in vitro native species conservation.

RESUMO Campomanesia rufa (O. Berg) Nied. é uma espécie mirtácea nativa do Cerrado brasileiro. Considerada uma espécie ameaçada de extinção, a propagação in vitro de C. rufa apresenta-se como uma alternativa para sua conservação. No entanto, a falta de protocolos eficazes para a desinfecção de microrganismos endofíticos naturalmente presentes nos tecidos vegetais dificulta o sucesso da micropropagação. Neste contexto, o objetivo deste estudo foi o isolamento, a identificação molecular e o controle de bactérias endógenas de C. rufa propagadas in vitro. Produtos de PCR purificados dos isolados bacterianos foram sequenciados pelo método de Sanger. As sequências obtidas foram alinhadas com sequências homólogas usando Basic Local Alignment Search Tool (BLAST) disponível na National Centre for Biotechnology Information (NCBI). As sequências obtidas neste estudo e as sequências homólogas listadas no GenBank foram alinhadas usando o software MEGA7 para elaborar árvores filogenéticas. Avaliaram-se a sobrevivência, a altura da parte aérea e a eficiência de antibióticos (estreptomicina, ampicilina e cloranfenicol) em diferentes concentrações no meio de cultura. A ocorrência de diferentes cepas de Bacillus sp. foi observada nos tecidos vegetais. Ao testar o controle de Bacillus sp. com antibióticos, o uso de 32 mg L-1 de ampicilina causou uma redução significativa na contaminação bacteriana sem afetar a sobrevivência do explante. Por outro lado, o uso de 256 mg L-1 de estreptomicina causou a maior redução na contaminação, embora esta concentração tenha sido letal para mais de 90% dos explantes. Este estudo é o primeiro relato sobre a ocorrência de bactérias endógenas e seu controle no contexto da conservação in vitro de espécies nativas.