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Anatomy of the lower hypogastric plexus applied to endometriosis: a narrative review
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Ribeiro-Julio, Gisele Silva

; Pereira, Jorge Alves

; Ribeiro, Eduardo

; Gallo, Carla M.

; Favorito, Luciano A.

International braz j urol

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jun 2023, Volume 49 N. 3 Pages 299 - 306

Abstract: En | Text: En | PDF: En

ABSTRACT Objective The objective of the present study is to evaluate the anatomy of the inferior hypogastric plexus, correlating it with urological pathologies, imaging exams and surgeries of the female pelvis, especially for treatment of endometriosis. Material and Methods We carried out a review about the anatomy of the inferior hypogastric plexus in the female pelvis. We analyzed papers published in the past 20 years in the databases of Pubmed, Embase and Scielo, and we included only papers in English and excluded case reports, editorials, and opinions of specialists. We also studied two human fixed female corpses and microsurgical dissection material with a stereoscopic magnifying glass with 2.5x magnification. Results Classical anatomical studies provide few details of the morphology of the inferior hypogastric plexus (IHP) or the location and nature of the associated nerves. The fusion of pelvic splanchnic nerves, sacral splanchnic nerves, and superior hypogastric plexus together with visceral afferent fibers form the IHP. The surgeon’s precise knowledge of the anatomical relationship between the hypogastric nerve and the uterosacral ligament is essential to reduce the risk of complications and postoperative morbidity of patients surgically treated for deep infiltrative endometriosis involving the uterosacral ligament. Conclusion Accurate knowledge of the innervation of the female pelvis is of fundamental importance for prevention of possible injuries and voiding dysfunctions as well as the evacuation mechanism in the postoperative period. Imaging exams such as nuclear magnetic resonance are interesting tools for more accurate visualization of the distribution of the hypogastric plexus in the female pelvis.

https://doi.org/10.1590/s1677-5538.ibju.2022.9980

Evaluation of laparoscopic knot training in a silicone model
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OSORIO, LUIZ FELIPE FERNANDES

; MACIEL, CLARICE FRAGA ESTEVES

; GALLO, CARLA BRAGA MANO

; SAMPAIO, FRANCISCO J.

Revista do Colégio Brasileiro de Cirurgiões

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2023, Volume 50 elocation e20233476

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Introduction: in videolaparoscopic surgery, movements are conducted from a twenty times magnified image of an indirect operative field. The video interface used assumes the need for depth perception using two dimensions instead of three. Objective: to evaluate the effectiveness of training to perform the laparoscopic knot in a silicone model, in 8-hour courses, and to analyze the correlation of learning outcomes with factors such as: sex, age, laterality, previous knowledge in endosuture and medical specialty. Material and Methods: in this prospective and randomized study, 56 students were evaluated, who took 8-hour courses, with groups of up to ten students. We used: a white box with camera, LCD screen and silicone piece. Four exercises were performed on the silicone mold: right hand, left hand, needle at 45° and back hand. Results: 56 students (mean age = 33.28 years). The female group, n=18, mean age 29.61 years, 17 right-handed and 1 left-handed. The male group, n=38, mean age 34.57 years, 35 right-handers, 1 left-handed and 2 ambidextrous. In both groups, no correlation was observed between the analyzes of well performed knots when correlated with the age or sex of the participants. Conclusion: laparoscopic knot training, in silicone molds, in 8 h courses, proved to be effective. Factors such as gender, age, laterality, previous knowledge in endosuture and medical specialty do not interfere with the learning results.

RESUMO Introdução: na cirurgia videolaparoscópica os movimentos são conduzidos a partir de uma imagem magnificada de vinte vezes de um campo operatório indireto. A interface de vídeo utilizada pressupõe a necessidade de percepção de profundidade utilizando duas dimensões ao invés de três. Objetivo: avaliar a eficácia do treinamento de execução do nó laparoscópico em modelo de silicone, em cursos de 8h, e analisar a correlação dos resultados de aprendizagem com fatores como: sexo, idade, lateralidade, conhecimentos prévios em endosutura e especialidade médica. Material e Métodos: Neste estudo, prospectivo e randomizado, foram avaliados 56 alunos, que realizaram cursos de 8h de duração, com turmas de até dez alunos. Foram utilizados: uma caixa branca com câmera, tela de LCD e peça de silicone. No molde de silicone foram executados quatro exercícios: mão direita, mão esquerda, agulha a 45° e back hand. Resultados: 56 alunos (média de idade = 33,28 anos). O grupo feminino, n=18, média de idade 29,61 anos, 17 destras e 1 canhota. O grupo masculino, n=38, média de idade 34,57 anos, 35 destros, 1 canhoto e 2 ambidestros. Em ambos grupos não foram observados nenhuma correlação entre as análises de nós bem executados quando correlacionados com a idade ou sexo dos participantes. Conclusão: o treinamento de nó laparoscópico, em moldes de silicone, em cursos de 8h de duração, mostrou-se eficaz. Fatores como sexo, idade, lateralidade, conhecimentos prévios em endosutura e especialidade médica, não interferem nos resultados do aprendizado.

https://doi.org/10.1590/0100-6991e-20233476-en

Kidney surface development in human fetuses: study applied to radiological diagnosis
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Favorito, Luciano A.

; Lobo, Marcio Luiz P.

; Fernandes, Ana Vitória

; Gallo, Carla M.

; Sampaio, Francisco J. B.

International braz j urol

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dec 2022, Volume 48 N. 6 Pages 930 - 936

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: To evaluate the anatomical aspects of the kidney surface in human fetuses during the second gestational trimester. Material and Methods: We studied 108 kidneys obtained from 54 human fetuses (29 males and 25 females). The kidney was dissected and the number of clefts was counted. The renal volume was also assessed. To compare the quantitative data in both sexes, the Students-t-test was used (p < 0.05). Simple linear correlations were calculated for all kidney measurements, according to fetal age. Statistical analysis was performed with the R program (Version 3.5.1). Results: The fetuses ranged in age between 11.4 to 23 weeks post-conception. The renal volume of the right kidney ranged from 0.09 to 2.397 cm (mean=0.8479) and the renal volume of the left kidney ranged from 0.07 to 2.416 cm (mean=0.8036). The mean number of renal clefts in fetuses studied was 15.25 (7 to 28). There was no statistical significant difference in renal clefts between the sides either in males (p = 0.646) or in females (p = 0.698). Also, there was no significant difference in the mean number of renal clefts between male and female fetuses in right kidney (p = 0.948) and in left kidney (p = 0.939). Conclusions: The number of renal clefts has a great variation, weak correlation and no tendency to decrease during the 2nd gestational trimester. The number of clefts in right kidney of total sample and female fetuses has a significant development with age.

https://doi.org/10.1590/s1677-5538.ibju.2022.9977

Epididymal disjunction anomalies in undescended testis – a factor associated with spermatic obstruction
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Logsdon, Natasha T.

; Gallo, Carla M.

; Sampaio, Francisco J. B.

; Favorito, Luciano A.

International braz j urol

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apr 2022, Volume 48 N. 2 Pages 336 - 346

Abstract: En | Text: En | PDF: En

ABSTRACT Background: To analyze the incidence of epididymal anomalies (EAs) associated to spermatic obstruction in patients with undescended testis (UT) according to testicular position and age. Materials and Methods: We studied 87 patients (110 testis) with cryptorchidism and analyzed the presence of EAs correlated with the testicular position, age and patency of the processus vaginalis (PV). To analyze the relations between the testis and epididymis we considered three situations: (a) Normal pattern: the epididymis was attached to the testis at the head and tail and epididymis totally attached to the testis; (b) EAs: when the epididymis was attached to the testis only at the head (Figure-1A) and (c) EAs associated to spermatic obstruction: epididymis was attached to the testis only at the tail (Figure-1B) and when there are no visible connection between testis and epididymis (Figure-1C). We used the Wilcoxon-Mann-Whitney test and the Chi-square test for contingency analysis (p <0.05). Results: The mean age of the patients was 5.18 years (SD=2.867). Of 110 testes analyzed, 14 were abdominal (12.72%); 83 inguinal (75.45%) and 13 suprascrotal (11.81%). Normal relationships between testis and epididymis were observed in 54 patients (62.1%) with no significant differences in relation to the patient's age (p=0.666). Epididymal tail disjunction was observed in 23 patients (26.44%), with no significant differences in relation to age (p=0.59). EAs associated to spermatic obstruction were observed in 16 patients (18.4%), also with no significant differences in relation to age (p=0.684). We did not observe significant correlation between the testis position and the incidence of EAs (p=0.119). We did not observe significant correlations between patency of the PV (64.7%) and incidence of EAs (p=0.742). Conclusions: Epididymal anomalies associated with spermatic obstruction are present in almost 20% of undescended testes, without significant correlation with age, testicular position and patency of the PV. This information needs to be correlated to the infertility risk of this congenital anomaly.

https://doi.org/10.1590/s1677-5538.ibju.2022.99.07

Vulnerabilities of nurses in an intensive care unit: an integrative review
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Vocci, Marcelli Cristine

; Gallo, Isabella Gandolfi

; Spiri, Wilza Carla

; Borgato, Maria Helena

; Fontes, Cassiana Mendes Bertoncello

Revista Brasileira de Enfermagem

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2021, Volume 74 N. 3 elocation e20200724

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivos: identificar as vulnerabilidades dos enfermeiros atuantes em unidade de terapia intensiva. Métodos: foi realizada revisão integrativa no período de 2006 a 2019, com busca nas plataformas/bases de dados LILACS, BDENF, SciELO, MEDLINE, Scopus, CINAHL e Web of Science. Foi utilizado o referencial teórico da ergologia, de Schwartz, e a pergunta norteadora “Quais são as principais vulnerabilidades dos enfermeiros atuantes em unidade de terapia intensiva?”. Resultados: onze artigos inclusos. Emergiram cinco temas de vulnerabilidades: física; emocional; processo de comunicação; processo de cuidar; e organizacional. Constatou-se que a vulnerabilidade organizacional se manteve como um fator comum e de intersecção no desenvolvimento das outras quatro. Conclusões: as vulnerabilidades encontradas relacionaram-se aos elementos: físico, emocional; de comunicação; do processo de cuidar; e organizacional da instituição. O aspecto organizacional fez intersecção com os outros, configurando-se como nuclear nesse contexto.

ABSTRACT Objectives: to identify vulnerabilities of nurses who work in an intensive care unit. Methods: an integrative review was held from 2006 to 2019, with searches on the platforms/databases LILACS, BDENF, SciELO, MEDLINE, Scopus, CINAHL, and Web of Science. The theoretical framework of ergology, by Schwartz, was used and the guiding question “What are the main vulnerabilities of nurses who work in an intensive care unit”? Results: eleven articles were included. Five topics of vulnerabilities emerged: physical, emotional, communication process, care process, and organizational. We observed that the organizational vulnerability was kept as a common and intersection factor in developing the other four. Conclusions: vulnerabilities found have a relationship to the physical, emotional, communication, care process, and institution organization elements. The organization aspect intersected with the others, configuring itself as the core in this context.

RESUMEN Objetivos: identificar las vulnerabilidades de los enfermeros actuantes en unidad de cuidados intensivos. Métodos: ha sido realizada revisión integrativa entre 2006 a 2019, con búsqueda en las plataformas/bases de datos LILACS, BDENF, SciELO, MEDLINE, Scopus, CINAHL y Web of Science. Ha sido utilizado el referencial teórico de la ergología, de Schwartz, y la pregunta de investigación “¿Cuáles son las principales vulnerabilidades de los enfermeros actuantes en unidad de cuidados intensivos?”. Resultados: once artículos inclusos. Emergieron cinco temas de vulnerabilidades: física; emocional; proceso de comunicación; proceso de cuidar; y organizacional. Constató que la vulnerabilidad organizacional se mantuvo como un factor común y de intersección en el desarrollo de las otras cuatro. Conclusiones: las vulnerabilidades encontradas se relacionaron a los elementos: físico, emocional; de comunicación; del proceso de cuidar; y organizacional de la institución. El aspecto organizacional hizo intersección con los otros, configurándose como nuclear en eso contexto.

https://doi.org/10.1590/0034-7167-2020-0724 173 downloads

Consenso argentino en gestión efectiva de clínicas de anticoagulación para uso de antagonistas de la vitamina K
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Ceresetto, José M

; Duboscq, Cristina

; Korin, Jorge

; Fondevila, Carlos

; Casais, Patricia

; Rossi, Andrea

; Scazziota, Alejandra

; Martinuzzo, Marta

; Aris Cancela, María Esther

; Rosa, Claudio

; Pombo, Gonzalo

; Guimelli, Carla

; Adamczuk, Yolanda

; Martí, Alejandra

; Penchasky, Diana

; Riveros, Dardo

; Puente, Dolores

; Celebrin, Lucía

; Molnar, Soledad

; Meschengieser, Susana

; Castro Ríos, Miguel

; Blanco, Alicia

; Hendler, Héctor

; Brodsky, Andrés

; Sánchez Luceros, Analía

; Negri Aranguren, Pedro

; Fedele, José Luis

; Merlo, Claudio

; Raña, Pablo

; Gumpel, Carina

; Colorio, Cecilia

; Grand, Beatriz

; Canónico, Virginia

; Rossi, Eleonora

; Colimodio, Paulo

; Orlando, Sergio

; Fassi, Daniel

; Arias, Mirta

; Viudez, Laura

; Farreras, Roberto

; Martínez, Pablo

; Ferro, Hugo

; Casali, Claudia

; Baques, Alejandra

; Arbesú, Guillermo

; Viñuales, Susana

; Medina, Fernanda

; Cortés, Verónica

; Gallo, María Del Carmen

; Ehelou, Ludmila

; Barrera, Luis

; Rey, Irene

; De Larrañaga, Gabriela

; Figueroa, Flavia

; Vilaseca, Alicia

; Kuri, Isabel

; Maneyro, Alberto

; Otaran, María

; Xavier, David Luis

; Garbiero, Susana

; Salviú, Julieta

; Fontenla, Patricia

; Eckhardt, Andrea

; Bomparola, Carolina

; Fornasiero, Laura

Medicina (Buenos Aires)

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sep 2020, Volume 80 Suppl. 4 Pages 1 - 26

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Treating an anticoagulated patient with vitamin K antagonists (VKA) remains a challenge, especially in areas where dicoumarins are still the first drug of choice due to the cost of other oral anticoagulants. Anticoagulation clinics have proven to be the most efficient and safe way to avoid thrombotic and hemorrhagic complications and to keep patients in optimal treatment range. However, they require adequate infrastructure and trained personnel to work properly. In this Argentine consensus we propose a series of guidelines for the effective management of the anticoagulation clinics. The goal is to achieve the excellence in both the clinical healthcare and the hemostasis laboratory for the anticoagulated patient. The criteria developed in the document were agreed upon by a large group of expert specialists in hematology and biochemistry from all over the country. The criteria presented here must always be considered when indicating VKA although they had to be adapted to the unequal reality of each center. Taking these premises into consideration will allow us to optimize the management of the anticoagulated patient with VKA and thus minimize thrombotic and hemorrhagic intercurrences, in order to honor our promise not to harm the patient.

Resumen El tratamiento de un paciente anticoagulado con antagonistas de la vitamina K (AVK) sigue siendo un desafío, especialmente en regiones donde, por el costo, los dicumarínicos son todavía la alternativa más buscada a la hora de elegir un anticoagulante oral. Las clínicas de anticoagulación han demostrado ser la forma más eficiente y segura de evitar complicaciones trombóticas y hemorrágicas y de mantener al paciente en rango óptimo de tratamiento. Sin embargo, requieren de una adecuada infraestructura y personal capacitado para que funcionen eficientemente. En este consenso argentino se propone una serie de parámetros para la gestión efectiva de una clínica de anticoagulación. El objetivo es lograr una elevada calidad desde el punto de vista clínico-asistencial a través de un laboratorio de hemostasia de excelencia. Los criterios desarrollados en el documento fueron consensuados por un amplio grupo de expertos especialistas en hematología y en bioquímica de todo el país. Estos criterios deben adaptarse a la irregular disponibilidad de recursos de cada centro, pero siempre se los debe tener en cuenta a la hora de indicar el tratamiento anticoagulante con estas drogas. Tener en consideración estas premisas nos permitirá optimizar la atención del enfermo anticoagulado con AVK y de esta forma minimizar las intercurrencias trombóticas y hemorrágicas a las que está expuesto, para así honrar nuestra promesa de no dañar al paciente.

375 downloads

Diagnóstico y tratamiento de la etapa aguda del accidente cerebrovascular isquémico: Guía de práctica clínica del Seguro Social del Perú (EsSalud)
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Sequeiros-Chirinos, Joel Mario

; Alva-Díaz, Carlos A.

; Pacheco-Barrios, Kevin

; Huaringa-Marcelo, Jorge

; Huamaní, Charles

; Camarena-Flores, Carla E.

; Durand-Castro, Walter S.

; Valencia-Chávez, Ana María

; Ecos-Quispe, Rosa L.

; Estupinan-Valdez, Paula I.

; Gallo-Guerrero, Marla

; Huamaní-Mendoza, Marlene Doris

; Mariños-Sánchez, Evelyn Vanessa

; Morón-Cabrera, María Elizabeth

; Pulachet-Contreras, Elena

; Ramos-Auccasi, Alfredo

; Rodríguez-Kadota, Liliana

; Saavedra-Rocha, César

; Chávez-Rimache, Lesly

; Timaná-Ruiz, Raúl

Acta Médica Peruana

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jan 2020, Volume 37 N. 1 Pages 54 - 73

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Objective: to provide evidence based clinical recommendations for the diagnosis and therapy of the acute stage of ischemic cerebrovascular accident in EsSalud. Materials and methods : a group for producing the guideline was conveyed, including specialized physicians and methodologists. The group prepared 8 clinical questions to be answered by the guideline. Systematic searches of previous reviews were performed, and - when it was deemed necessary - primary studies in Medline and the Cochrane Controlled Registry of Trials for 2018 were reviewed. Evidence was selected aiming to respond each one of the proposed clinical questions. Certainty of the evidence was determined using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) methodology. In periodic work sessions, the group used GRADE methodology for reviewing the evidence and generated recommendations, good clinical practice items, and designed flow charts for both diagnosis and therapy. Finally, the guide was approved with the decree N° 128-IETSI-ESSALUD-2019. Results : this clinical practice guideline focused in 8 clinical questions, which were divided into four topics: screening, diagnosis, therapy, support and rehabilitation. On the basis of these questions, 28 recommendations were formulated (8 strong and 20 conditional), 38 items for good clinical practice, 1 implementation note, and 2 flow charts. Conclusion : this paper summarizes both evidence based methodology and conclusions from a new clinical practice guide for diagnosis and therapy of the acute stage of a cerebrovascular accident in EsSalud.

RESUMEN Objetivo: proveer recomendaciones clínicas basadas en evidencia para el diagnóstico y tratamiento de la etapa aguda del ataque cerebrovascular isquémico en EsSalud. Materiales y métodos: se conformó un grupo elaborador de la guía (GEG) que incluyó médicos especialistas y metodólogos. El GEG formuló 8 preguntas clínicas a ser respondidas por la presente GPC. Se realizó búsquedas sistemáticas de revisiones sistemáticas y -cuando fue considerado pertinente- estudios primarios en Medline y Cochrane Controlled Register of Trials durante el 2018. Se seleccionó la evidencia para responder cada una de las preguntas clínicas planteadas. La certeza de la evidencia fue evaluada usando la metodología Grading of Recommendations Assessment, Development, and Evaluation (GRADE). En reuniones de trabajo periódicas, el GEG usó la metodología GRADE para revisar la evidencia y formular las recomendaciones, los puntos de buenas prácticas clínicas y el flujograma de diagnóstico y tratamiento. Finalmente, la GPC fue aprobada con Resolución N° 128-IETSI-ESSALUD-2019. Resultados: la presente GPC abordó 8 preguntas clínicas, divididas en cuatro temas: tamizaje, diagnóstico, tratamiento, soporte y rehabilitación. En base a estas preguntas se formularon 28 recomendaciones (8 fuertes y 20 condicionales), 38 puntos de buena práctica clínica, 1 nota de implementación y 2 flujogramas. Conclusión: el presente artículo resume la metodología y las conclusiones basadas en evidencias de la GPC para el diagnóstico y tratamiento de la etapa aguda del ataque cerebrovascular isquémico en EsSalud.

https://doi.org/10.35663/amp.2020.371.869

Expression of stromal elements of prostatic adenocarcinoma in different gleason scores
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Osorio, Clarice Fraga Esteves Maciel

; Costa, Waldemar Silva

; Gallo, Carla Braga Mano

; Sampaio, Francisco José Barcellos

Acta Cirúrgica Brasileira

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oct 2019, Volume 34 N. 10 elocation e201901005

Abstract: En | Text: En | PDF: En

Abstract Purpose: To quantify and compare the expression of stromal elements in prostate adenocarcinoma of different Gleason scores with non-tumor area (control). Methods: We obtained 132 specimens from samples of prostate peripheral and transition zone. We analyzed the following elements of the extracellular matrix: collagen fibers, elastic system, smooth muscle fibers and blood vessels. The tumor area and non-tumor area (control) of the TMA (tissue microarray) were photographed and analyzed using the ImageJ software. Results: The comparison between the tumor area and the non-tumor area showed significant differences between stromal prostate elements. There was an increase of collagen fibers in the tumor area, mainly in Gleason 7. Elastic system fibers showed similar result, also from the Gleason 7. Blood vessels showed a significant increase occurred in all analyzed groups. The muscle fibers exhibited a different behavior, with a decrease in relation to the tumor area. Conclusions: There is a significant difference between the extracellular matrix in prostate cancer compared to the non-tumor area (control) especially in Gleason 7. Important modifications of the prostatic stromal elements strongly correlate with different Gleason scores and can contribute to predict the pathological staging of prostate cancer.

https://doi.org/10.1590/s0102-865020190100000005 784 downloads

Effect of a high-fat diet on the rat bladder wall and bioactive action of Brazil nut oil
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Souza, Aline Costa de

; Gallo, Carla Braga Mano

; Passos, Magna Cottini da Fonseca

; Croccia, Carolina

; Miranda, Glauciane Lacerda

; Sampaio, Francisco José Barcellos

; Gregório, Bianca Martins

International braz j urol

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jan 2019, Volume 45 N. 1 Pages 161 - 168

Abstract: En | Text: En | PDF: En

ABSTRACT High-fat diet-induced obesity is associated with metabolic disorders. The Brazil nut has bioactive substances and has been used to control the damage caused by obesity in several organs. The work intended to show the damage caused by high-fat diet in the bladder wall and if the Brazil nut oil added to the diet could ameliorate or reverse this effect. Sixty-day-old rats were divided into two groups: C (control, n = 30) and HF (high-fat, n = 30) diets. At 90 days, 10 animals of each group were sacrificed. The others were divided into 4 groups: C and HF (animals that maintained their previous diet, n = 10 for each group) and C / Bno and HF / Bno (animals whose control or high-fat diet was supplemented by Brazil nut oil, n = 10 for each group). Sacrifice occurred at 120 days, and the bladders were removed and analyzed. Epithelial height was increased in the HF compared to the C group. In contrast, the C / Bno had a lower epithelial height compared to the others. The percentage of collagen between the detrusor muscle fibers was significantly greater in C / Bno, HF and HF / Bno than in control group. The HF had a larger muscle fiber diameter than the C group, while the C / Bno presented lower values than the HF and HF / Bno groups. HF diets induced bladder wall damage. These changes in the rat's bladder wall were partially reversed by the Bno.

https://doi.org/10.1590/s1677-5538.ibju.2018.0547 641 downloads

10.

Relación entre estrés laboral y burnout en una muestra de controladores de tráfico aéreo en Chile
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Aguirre Mas, Carla

; Gallo, Agustina

; Ibarra, Alexander

; Sánchez García, José Carlos

Ciencias Psicológicas

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nov 2018, Volume 12 N. 2 Pages 239 - 248

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen: Esta investigación tiene por objetivo hallar la relación entre el burnout, el estrés laboral, la satisfacción laboral y el conflicto trabajo-familia en controladores de tráfico aéreo, como variables diferenciales: edad, sexo, estado civil, permanencia laboral, tipo de contrato y presencia o ausencia de hijos. Esta investigación es de tipo descriptivo-correlacional de corte transversal. Se aplicaron los Cuestionario: MBI-GS de Burnout, Instrumento de Medición de detonantes de Estrés Laboral para pilotos (IMDELP) y el Cuestionario Conflicto Trabajo-Familia. La muestra fue de 161 personas, mediante muestreo intencionado. Los resultados indican que se obtuvo un nivel moderado de burnout, estrés laboral y conflicto trabajo-familia, existiendo diferencias significativas y directamente proporcionales a la hora de analizar las dimensiones del burnout relacionándolas con extensión de las jornadas de trabajo y agotamiento, agotamiento y conflicto trabajo-familia, estrés laboral y cinismo

Abstract: The purpose of the present study was to identify the relation between burnout syndrome, work stress, work satisfaction and work-family conflict in a sample of air traffic controllers, taking in consideration the following variables: age, sex, civil status, work permanence, contract type and presence or absence of children. In this descriptive-correlational study we used the: MBI-GS of Burnout Questionnaire, detonating questionnaire Instrument of measurement of Work Stress for pilots (IMDELP), and the Work- Family Conflict Questionnaire. The sample was 161 Air Traffic Controllers, through intentional sampling. The results indicate a moderate level of burnout, work-related stress and work-family conflict. There were significant and directly proportional differences when analyzing the dimensions of burnout, relating them to the length of work and exhaustion, exhaustion and conflict work-family, work stress and cynicism

https://doi.org/10.22235/cp.v12i2.1688 7774 downloads

11.

Biochemical and molecular characterization of 3-Methylcrotonylglycinuria in an Italian asymptomatic girl
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Cozzolino, Carla

; Villani, Guglielmo RD

; Frisso, Giulia

; Scolamiero, Emanuela

; Albano, Lucia

; Gallo, Giovanna

; Romanelli, Roberta

; Ruoppolo, Margherita

Genetics and Molecular Biology

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jun 2018, Volume 41 N. 2 Pages 379 - 385

Abstract: En | Text: En | PDF: En

Abstract 3-Methylcrotonylglycinuria is an organic aciduria resulting from deficiency of 3-methylcrotonyl-CoA carboxylase (3-MCC), a biotin-dependent mitochondrial enzym carboxylating 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA during leucine catabolism. Its deficiency, due to mutations on MCCC1 and MCCC2 genes, leads to accumulation of 3-methylcrotonyl-CoA metabolites in blood and/or urine, primarily 3-hydroxyisovaleryl-carnitine (C5-OH) in plasma and 3-methylcrotonyl-glycine (3-MCG) and 3-hydroxyisovaleric acid (3-HIVA) in the urine. The phenotype of 3-MCC deficiency is highly variable, ranging from severe neurological abnormalities and death in infancy to asymptomatic adults. Here we report the biochemical and molecular characterization of an Italian asymptomatic girl, positive for the newborn screening test. Molecular analysis showed two mutations in the MCCC2 gene, an already described missense mutation, c.691A > T (p.I231F), and a novel splicing mutation, c.1150-1G > A. We characterized the expression profile of the splice mutation by functional studies.

https://doi.org/10.1590/1678-4685-gmb-2017-0093 1145 downloads

12.

Relación entre asimetría fluctuante y el tratamiento hormonal, cirugía-ortodoncia maxilofacial, traumatismos y malformaciones craneofaciales
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Quinto-Sánchez, Mirsha

; Cintas, Celia

; Ramallo, Virginia

; Cerqueira, Caio Cesar Silva de

; Gomez-Valdés, Jorge

; Acuña-Alonzo, Victor

; Adhikari, Kaustubh

; Everardo, Paola

; Avila, Francisco de

; Jaramillo, Carla

; Arias, Williams

; Fuentes, Macarena

; Hünemeier, Tábita

; Gallo, Carla

; Poletti, Giovani

; Rosique, Javier

; Schuler-Faccini, Lavinia

; Bortolini, Maria Catira

; Canizales-Quinteros, Samuel

; Rothhammer, Francisco

; Bedoya, Gabriel

; Ruiz-Linares, Andres

; Gonzalez-José, Rolando

Revista argentina de antropología biológica

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jan 2018, Volume 20 N. 1 Pages 1 - 16

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

In this work we test for the putative association between facial fluctuating asymmetry (FFA) and hormone treatments, maxillofacial surgery, orthodontics, injuries, and malformations. A protocol of five photographs and photogrammetric reconstruction was implemented to place thirty-four 3D landmarks in 3162 individuals aged between 18 and 85 years, belonging to the CANDELA initiative. A Procrustes ANOVA test was used to obtain individual facial fluctuating asymmetry scores. One way ANOVA, Welch, and Levene tests were conducted to explore the potential differences between mean and variance of the response variables. Our results indicate that women who received some hormonal treatment showed higher fluctuating facial asymmetry scores in relation to the unaffected group, this being persistent once the effects of heterozygosity (genetic ancestry) and further variables had been statistically controlled. The shape changes corresponding to this association are focused on the chin, jaw, lower lip, prefrontal region, nose, and ears. Previous reports suggested a potential relationship between facial asymmetry and hormone levels, but to the best of our knowledge there are no reports indicating the causation underlying the association detected here. This report is one more evidence of the association between hormone intake and facial asymmetric features in urban admixed Latin American populations. Rev Arg Antrop Biol 20(1), 2018. doi:10.17139/raab.2018.0020.01.06

En este trabajo se evalúa la relación entre la asimetría fluctuante facial (AFF) y los tratamientos hormonales, cirugías maxilofaciales, ortodoncia, traumatismos y malformaciones. En el marco del proyecto CANDELA, se tomaron cinco fotografías faciales de 3162 voluntarios entre los 18 y 85 años. Por fotogrametría se colocaron 34 landmarks o puntos en 3D y mediante el método Procrustes ANOVA se obtuvieron valores individuales de asimetría fluctuante facial. Se realizó una prueba de ANOVA de una vía y la prueba de Welch y Levene para conocer las diferencias entre media y varianza de los valores de asimetría facial y las variables respuesta. También, se caracterizó la variación morfológica del componente asimétrico de la forma facial mediante técnicas multivariadas sobre los grupos que resultaran diferentes significativamente. Las mujeres que reportaron haber recibido algún tipo de tratamiento hormonal mostraron mayores valores de asimetría fluctuante facial respecto al grupo sin tratamiento. Esta asociación se mantuvo una vez removido el efecto de la heterocigosidad (como indicador de la ancestría) y sin interactuar con el resto de covariables incluidas en el análisis. Los cambios morfológicos asociados a este factor se concentran en el mentón, maxilar labio inferior, región perifrontal, región nasal y orejas. Algunos trabajos anteriores dieron cuenta de la posible relación entre la asimetría facial y los niveles de hormonas, pero no hay estudios que sustenten la relación causal o directa entre la asociación aquí planteada. El presente trabajo es una evidencia más de la asociación entre el consumo de hormonas y modificaciones de caracteres faciales en poblaciones urbanas mestizas latinoamericanas. Rev Arg Antrop Biol 20(1), 2018. doi:10.17139/raab.2018.0020.01.06

https://doi.org/10.17139/raab.2018.0020.01.06 526 downloads

13.

The prostate after castration and hormone replacement in a rat model: structural and ultrastructural analysis
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Felix-Patrício, Bruno

; Miranda, Alexandre F.

; Medeiros Jr., Jorge L.

; Gallo, Carla B. M.

; Gregório, Bianca M.

; Souza, Diogo B. de

; Costa, Waldemar S.

; Sampaio, Francisco J. B.

International braz j urol

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oct 2017, Volume 43 N. 5 Pages 957 - 965

Abstract: En | Text: En | PDF: En

ABSTRACT Purpose: To evaluate if late hormonal replacement is able to recover the prostatic tissue modified by androgenic deprivation. Materials and Methods: 24 rats were assigned into a Sham group; an androgen deficient group, submitted to bilateral orchiectomy (Orch); and a group submitted to bilateral orchiectomy followed by testosterone replacement therapy (Orch+T). After 60 days from surgery blood was collected for determination of testosterone levels and the ventral prostate was collected for quantitative and qualitative microscopic analysis. The acinar epithelium height, the number of mast cells per field, and the densities of collagen fibers and acinar lumen were analyzed by stereological methods under light microscopy. The muscle fibers and types of collagen fibers were qualitatively assessed by scanning electron microscopy and polarization microscopy. Results: Hormone depletion (in group Orch) and return to normal levels (in group Orch+T) were effective as verified by serum testosterone analysis. The androgen deprivation promoted several alterations in the prostate: the acinar epithelium height diminished from 16.58±0.47 to 11.48±0.29μm; the number of mast cells per field presented increased from 0.45±0.07 to 2.83±0.25; collagen fibers density increased from 5.83±0.92 to 24.70±1.56%; and acinar lumen density decreased from 36.78±2.14 to 16.47±1.31%. Smooth muscle was also increased in Orch animals, and type I collagen fibers became more predominant in these animals. With the exception of the densities of collagen fibers and acinar lumen, in animals receiving testosterone replacement therapy all parameters became statistically similar to Sham. Collagen fibers density became lower and acinar lumen density became higher in Orch+T animals, when compared to Sham. This is the first study to demonstrate a relation between mast cells and testosterone levels in the prostate. This cells have been implicated in prostatic cancer and benign hyperplasia, although its specific role is not understood. Conclusion: Testosterone deprivation promotes major changes in the prostate of rats. The hormonal replacement therapy was effective in reversing these alterations.

https://doi.org/10.1590/s1677-5538.ibju.2016.0484 1309 downloads

14.

Portadores faríngeos de Streptoccocus pneumoniae en dos grupos poblacionales. Ciudad Bolivar, Estado Bolívar, Venezuela
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Requena-Certad, Ixora

; Pacheco-Urbina, Carla

; Orta, María de los Angeles

; Marcano-Gallo, N.

; Salazar-González, R.

Revista biomédica

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apr 2017, Volume 28 N. 1 Pages 39 - 60

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Introduction Streptococcus pneumonia is a pathogen for humans that requires a prior pharyngeal colonization to cause disease, whose mortality is accentuated in children 6 years and older than 65 years of age. Objective To assess the prevalence of pharyngeal carriage of S pneumonia in two population groups in Ciudad Bolívar, Bolívar State. Methodology . During the years 2009 and 2010, two groups were evaluated: one which included 66 senior individuals institutionalized in the asylum "San Vicente de Paúl" and the geriatric "Carlos Fragachan", with an average of 75 years ± 6 years age and another that it comprised preschool and school aged 2 to 12 hospitalized in Pediatrics and the area of the University Hospital emergency services "Ruiz and Páez". Each individual took a pharyngeal sample, which was processed according to the guidelines established by the American society for of microbiology. 80 exudates from pharyngeal origin were studied. Results Between elderly individuals 10 cases were identified (15.15%) of S. pneumonia pharyngeal carriers. 70% (n = 7) corresponded to the male gender. In all the ages were diagnosed cases, often observed in the group between 71 to 80 years of age (n = 4; 40%). 46.2% of pharyngeal carriers concerned smoking history. Were observed significant statistical differences between the pneumococcus`s state carrier and type II diabetes mellitus and vaccination´s state against the bacterium, In the group of preschool and school inpatients, 3 strains of S. pneumonia that accounted for 3.75% of the total were isolated. The highest percentage of positive specimens was obtained in the group of 2 to 4 years, with a predominance of the male gender (2.5%). Statistically significant differences were observed in the hospitalized children, between the carrier´s state and personal history as asthma, cold common, urinary tract infection and infection of skin and tissue soft. They were not vaccinated against the pneumococcus or met only a single dose. Both studies identified 13 strains of Streptococcus pneumoniae, which showed a profile of resistance to penicillin, by method of disk Oxacillin, of 50%; While all of the isolates were with high resistance to macrolides and clindamycin, sulfonamides, and sensitive to Vancomycin. Conclusion We identified a low prevalence of individuals colonized by Streptococcus pneumoniae, however should be consider the research of colonization of the pharynx by Pneumococcus as a good method to be simple and easy to obtain bacterial samples and to reflect the progression of bacterial resistance in risk groups.

Resumen Introducción Streptococcus pneumoniae es un patógeno para el ser humano que precisa de una previa colonización faríngea para causar enfermedad, cuya morbimortalidad se acentúa en los menores de 6 años y los mayores de 65 años de edad. Objetivo Determinar la prevalencia de portadores faríngeos de Streptococcus pneumoniae en dos grupos poblacionales en Ciudad Bolívar, Estado Bolívar. Metodología Durante los años 2009 a 2010, se evaluaron dos grupos: uno que incluyó a 66 individuos de la tercera edad institucionalizados en el Asilo “San Vicente de Paúl” y Geriátrico “Carlos Fragachan”, con una edad promedio de 75 años ± 6 años y otro que abarcó a preescolares y escolares de 2 a 12 años hospitalizados en los Servicios de Pediatría y del área de emergencia del Complejo Hospitalario Universitario “Ruíz y Páez”. A cada individuo se le tomó una muestra faríngea, la cual fue procesada según los lineamientos establecidos por la Sociedad Americana de Microbiología. Se estudiaron 80 exudados de origen faríngeo. Resultados En el grupo de los individuos de la tercera edad se identificaron 10 casos (15,15%) de portadores faríngeos de S. pneumoniae. El 70% (n=7) correspondía al género masculino. En todas las edades se diagnosticaron casos, frecuentemente observados en el grupo entre 71 a 80 años de edad (n=4; 40%). El 46,2% de los portadores faríngeos de la tercera edad refirieron antecedentes tabáquicos. Se observaron diferencias estadísticas significativas entre el estado de portador de neumococo y la diabetes mellitus tipo II y estado de vacunación contra la bacteria, En el grupo de los pacientes preescolares y escolares hospitalizados, se aislaron 3 cepas de S. pneumoniae que representó el 3,75% del total. El mayor porcentaje de muestras positivas se obtuvo en el grupo de 2 a 4 años, con predominio del género masculino (2,5%). En el grupo de la población infantil hospitalizada, se observaron diferencias estadísticamente significativas entre el estado de portador y antecedentes personales como asma, resfriado común a repetición, infección urinaria e infección de piel y tejido blando. No estaban vacunados contra el neumococo o solo cumplieron una sola dosis. En ambas investigaciones se determinaron 13 cepas de Streptococcus pneumoniae, las cuales mostraron un perfil de resistencia a la Penicilina, por método del disco de Oxacilina, del 50%; mientras que la totalidad de las cepas aisladas resultaron con alta resistencia a Macrólidos, Clindamicina y Sulfamidas, y sensibles a Vancomicina. Conclusión Se identificó una baja prevalencia de individuos colonizados por Streptococcus pneumoniae, sin embargo, se debe considerar la investigación de colonización faríngea por neumococo como un buen método por ser simple y fácil de obtener muestras bacterianas y poder reflejar la progresión de la resistencia bacteriana en grupos de riesgo.

https://doi.org/10.32776/revbiomed.v28i1.562 348 downloads

15.

Análise da microdureza Vickers de zircônia Y-TZP pré-sinterizada para a usinagem e posterior aplicação como copings
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Kavashima, Lieca Hassegawa

; Sanches, Matheus Gallo

; Sousa, Edson Antonio Capello

; Ramos, Carla Müller

; Borges, Ana Flávia Sanches

; Fortulan, Carlos Alberto

; Foschini, Cesar Renato

Matéria (Rio de Janeiro)

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2017, Volume 22 N. 2 elocation e11817

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A zircônia Y-TZP é muito utilizada na confecção de coping como material estrutural para próteses odontológicas devido à sua cor branca/perolada opaca e resistência mecânica. Estas próteses são confeccionadas por usinagem com apoio de um software de CAM (Computer Aided Manufacturing), a partir de blocos prensados e pré-sinterizados. A pré-sinterização consiste em aquecer os blocos compactados a altas temperaturas, com uma taxa lenta de aquecimento até o início da formação dos pescoços entre as partículas, quando proporciona dureza/resistência e usinabilidade. Uma contradição, pois a dureza/resistência mecânica é necessária para proporcionar estabilidade as forças de corte, mas se muito duro, pode dificultar a usinagem então se deve encontrar uma faixa de compromisso de temperatura entre estabilidade e usinabilidade. Dessa forma, o objetivo do presente trabalho foi investigar a relação entre a microdureza e temperatura de pré-sinterização da zircônia Y-TZP e seu comportamento ao ser submetido à usinagem. O ensaio de microdureza foi escolhido como representante de resistência mecânica por ser não destrutivo e de resultado imediato. O pó de zircônia experimental foi submetido à prensagem uniaxial seguido de prensagem isostática a frio à 200 MPa e pré-sinterização entre 900°C e 950°C. Os blocos foram cortados e submetidos ao teste de estabilidade na usinagem e ensaio de microdureza Vickers sendo os dados submetidos ao teste de “normalidade” de Anderson-Darlin com p > 0,05. Foi utilizada ANOVA de um fator com o teste de Tukey-Kramer para verificar as diferenças entre as médias dos grupos. A investigação demonstrou que a diferença de 50°C na temperatura de pré-sinterização, acima de 900°C afetou significativamente a microdureza, contudo os tempos de patamar 2 e 4 horas não a influenciou significativamente. A maior temperatura de pré-sinterização dos grupos experimentais testados possibilitou um melhor comportamento na usinagem com o apoio de um software CAM, sendo a microdureza similar ao material comercial ZL.

ABSTRACT Y-TZP zirconia is widely used in the manufacture of coping as a structural material for dental prosthesis due to its opaque white/pearl color and mechanical strength. Dentures are made by machining with cam (computer aided manufacturing) software support, from pressed and pre-sintered blocks. Pre-sintering consists of heating the compressed blocks to high temperatures, with a slow heating rate until the neck formation among particles, when this provides hardness/strength and machinability. There is a contradiction, because the mechanical hardness/strength is necessary to provide stability to the cutting forces, but if it is very hard, machining can be difficult, therefore must be found a compromise zone of temperature between stability and machinability. In this way, the purpose of this study was to investigate the relationship between microhardness and the pre-sintering temperature of experimental Y-TZP zirconia and its behavior to be subjected to machining. The microharness testing was chosen as representative of mechanical strength once it is nondestructive and with immediate result. The experimental zirconia powder was subjected to uniaxial pressing, subsequently cold isostatic pressing of 200 MPa and pre-sintering between 900°C and 950°C. Blocks were cut and subjected to machining stability testing and vickers microhardnes testing which the data were subjected to the anderson-darlin (p > 0.05) test for “normality”. Anova's “one way” with tukey-kramer test was used to verify the differences among the means of groups. The investigation showed that the difference of 50°C in pre-sintering temperature above 900°C significantly affect the hardness, whereas 2 to 4 hour time did not significantly affect material microhardness. The highest pre-sintering temperature of the tested experimental groups enabled better performance in machining with cam software support, which provides similar microhardness to commercial ZL material.

https://doi.org/10.1590/s1517-707620170002.0149 2219 downloads

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