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1.
Impact of Aggregation and Compression on Cluster-Based Wireless Sensor Networks
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Abstract: In Wireless Sensor Networks (WSNs), a clustered architecture is generally used to reduce energy consumption irrespectively of the application of the system. We prove in this work that, a clustered network only reduces energy consumption if aggregation or compression functions are enabled. Furthermore, a clustered network would consume much more energy if clustering techniques without the use of aggregation/compression (or a low aggregation coefficient) due to the extra consumption in the cluster formation phase. As an additional feature, a general energy consumption model based on the notion of energy units is developed that can be easily extrapolated to either theoretical or experimental values. Hence, the proposed analytical framework is valid for any commercial node or energy consumption model.
https://doi.org/10.13053/cys-25-4-3882
4 downloads
2.
Análisis multicriterio para la identificación de áreas agroecológicas para el centro experimental Cota Cota, Ciudad de La Paz-Bolivia
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Revista de Investigación e Innovación Agropecuaria y de Recursos Naturales
- Journal Metrics
RESUMEN La presente investigación se llevó a cabo en el Centro Experimental Cota Cota perteneciente a la Facultad de Agronomía de la Universidad Mayor de San Andrés, con el objetivo de identificar: zonas agroecológicas, de uso forestal y de conservación, a través del análisis multicriterio. Es así que, para el trabajo de investigación se tomó seis muestras de suelo extraídas de calicatas con la profundidad de un metro, previamente elaboradas dentro del perímetro del Centro Experimental bajo los criterios de pendiente, proximidad al río y plan del uso del suelo; dichas muestras se llevaron a laboratorio y el resultado del análisis físico y químico fue mapeado a través de un software que aplique Sistemas de Información Geográfica (SIG), haciendo uso de las herramientas de interpolación espacial de datos Kriging e IDW. Los suelos más aptos para el uso agroecológico, son aquellos ubicados según el centroide generado por computadora en la siguiente coordenada: latitud 16° 32' 10.7'' Sur y longitud 68° 3' 51.2'' W. Indicando un suelo con 10-30 % de pendiente, pH neutro (6.54-7.14), un NDVI mayor a 0.4, textura franco arcillosa, potasio intercambiable 0.6-1 meq 100gS°-1, la materia orgánica es de 0.55-1.5%, el porcentaje de nitrógeno total esta entre los 0.2-0.3%, el fósforo disponible supera las 50 ppm, finalmente la capacidad de intercambio catiónico (CIC) entre 17.50-20.64 meq 100gS°-1. Según los parámetros utilizados en el análisis multicriterio, un suelo apto para la agroecología no necesita tener el máximo nivel de clasificación en sus propiedades. Solo el 1.1 % de toda el área total es apta, lo cual corresponde a aproximadamente 1 800 m2, mas el resto son forestales y para conservación.
ABSTRACT This research was carried out at the Cota Cota Experimental Center belonging to the Faculty of Agronomy of the Universidad Mayor de San Andrés, with the objective of identifying agroecological, forest use and conservation zones through multicriteria analysis. Thus, for the research work, six soil samples were taken from pits with a depth of one meter, previously prepared within the perimeter of the Experimental Center under the criteria of slope, proximity to the river and land use plan; these samples were taken to the laboratory and the result of the physical and chemical analysis was mapped through a software that applies Geographic Information Systems (GIS), using the Kriging and IDW data spatial interpolation tools. The soils most suitable for agroecological use are those located according to the computer-generated centroid at the following coordinates: latitude 16° 32' 10.7'' South and longitude 68° 3' 51.2'' W. Indicating a soil with 10-30 % slope, neutral pH (6.54-7.14), NDVI greater than 0.4, clay loam texture, exchangeable potassium 0.6-1 meq 100gS°-1, organic matter is 0.55-1.5%, the percentage of total nitrogen is between 0.2-0.3%, available phosphorus exceeds 50 ppm, finally the cation exchange capacity (CEC) is between 17.50-20.64 meq 100gS°-1. According to the parameters used in the multi-criteria analysis, a soil suitable for agroecology does not need to have the highest level of classification in its properties. Only 1.1% of the total area is suitable, which corresponds to approximately 1 800 m2, but the rest is forestry and conservation.
3.
Ensayes sísmicos en mesa vibradora de un edificio miniatura de 5 niveles con aisladores de base
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Abstract Shaking table tests were conducted in previous research at the National University of Mexico (UNAM) on a miniature steel building named EM2, using a strong ground motion record. This building represented a conventional building. This paper describes the results of a second test unit called EM2-I, tested in the same shaking table with the same ground motion used in the test of EM2. The test unit with isolators, EM2-I, had a superstructure identical to that of the test unit EM2 but with base isolators of double friction pendulum type. Measured maximum interstory drifts were equal to 5.3% and 0.97% for test units EM2 and EM2-I, respectively. Measured maximum global drift was equal to 4% and 0.63% for test units EM2 and EM2-I, respectively. The maximum seismic coefficient measured in the isolated superstructure was equal to 26 % of the maximum value for this parameter measured in test unit EM2. Implications of the results in this research are discussed in this paper, showing the importance of using isolated structures as an efficient solution for resisting strong earthquakes and capable of immediate occupancy.
Resumen En una investigación anterior realizada en mesa vibradora en el Instituto de Ingeniería de la UNAM, se efectuaron ensayes de un edificio miniatura de acero con base fija, sin aisladores, denominado EM2, con un registro de aceleraciones que llevó al espécimen a una respuesta del tipo no lineal. En la investigación que se describe en este artículo, se ensayó un segundo espécimen denominado EM2-I, en la misma mesa vibradora y con el mismo registro empleado en la investigación anterior. El espécimen EM2-I, tuvo la misma superestructura que el espécimen EM2, pero con aisladores de base del tipo doble péndulo de fricción. La máxima distorsión de entrepiso medida fue igual a 5.3% y 0.97% para los especímenes EM2 y EM2-I, respectivamente. La máxima distorsión global medida fue igual a 4% y 0.63% para los especímenes EM2 y EM2-I, respectivamente. El máximo coeficiente sísmico medido en la superestructura aislada fue igual al 26 % del máximo valor medido en el espécimen EM2. Los resultados encontrados en esta investigación muestran la importancia del uso de estructuras aisladas como una solución eficiente para que después de terremotos las estructuras puedan ser ocupadas de manera inmediata.
https://doi.org/10.18867/ris.105.559
179 downloads
4.
Prostate cancer early detection among primary care physicians in Mexico: A cross-sectional study
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Basulto-Martínez, Mario
; Ojeda-Pérez, Jorge E.
; Velueta-Martínez, Iván A.
; Cueto-Vega, Guillermo J.
; Flores-Tapia, Juan P.
; González-Losa, María del Refugio
.
Abstract Aim: This study aims to assess primary care physicians (PCPs) knowledge and skills regarding prostate cancer early detection (PCa-ED). Materials and methods: A survey about knowledge and skills of PCa-ED was delivered to PCP. Logistic regression analysis was conducted for the propensity of PCP to test prostatic specific antigen (PSA) on asymptomatic men. Results: The survey was completed by 170 PCP. Score on risk factors knowledge was 51.5 ± 15.7% a better score was not associated with conducting PCa-ED (p = 0.674). The 40.6% answered having an institutional program on PCa-ED and 86% having access to PSA testing. Testing PSA on asymptomatic men was found in 40%. Moreover, 61.2% do not performed any digital rectal examination for PCa-ED, and this was not associated with preventing factors such as lack of space, time, and assistance (p > 0.05). Fewer years in practice and being a family medicine resident were associated with a less likelihood of testing PSA in asymptomatic men. The only associated factor in the multivariable model was having access to PSA testing (odds ratio: 3.36 confidence interval 95% 1.54-7.30) p = 0.002). Conclusions: A low rate of PCP performs PCa-ED and using concepts outside evidence-based recommendations. A national program on PCa-ED and continuing medical education for PCP are a promising strategy to improve PCa-ED.
Resumen Objetivo: Evaluar el conocimiento y las habilidades de los médicos de primer contacto en la detección oportuna del cáncer de próstata (DO-CaP). Método: Se aplicó una encuesta a médicos de primer contacto. Se realizó un análisis de regresión logística evaluando la propensión de los médicos a medir el antígeno prostático específico (APE) en sujetos asintomáticos. Resultados: Contestaron 170 médicos y la calificación del conocimiento sobre factores de riesgo fue de 51.5 ± 15.7%, pero una mejor calificación no se asoció con realizar DO-CaP (p = 0.674). El 40.6% respondió contar con un programa institucional en DO-CaP y un 86% con acceso a la prueba de APE. El 40% medían el APE en sujetos asintomáticos. El 61.2% no realizaba ningún examen digital rectal para DO-CaP, y esto no se asoció con factores limitantes como falta de tiempo, espacio o asistencia (p > 0.05). Menos años en práctica y ser residente de medicina familiar disminuyeron la probabilidad de determinar el APE en asintomáticos. El único factor asociado en el análisis multivariado fue el acceso a la prueba de APE (odds ratio: 3.36; intervalo de confianza del 95%: 1.54-7.30; p = 0.002). Conclusión: Una baja proporción de médicos de primer contacto realizan DO-CaP y utilizan conceptos alejados de la evidencia científica. Un programa nacional en DO-CaP y de educación continua para médicos de primer contacto es una estrategia prometedora para mejorar la DO-CaP.
https://doi.org/10.24875/ciru.20000232
13 downloads
5.
Dietary patterns in Mexican preschool children are associated with stunting and overweight
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Flores, Mario E.
; Rivera-Pasquel, Marta
; Macías, Nayeli
; Sánchez-Zamorano, Luisa María
; Rodríguez-Ramírez, Sonia
; Contreras-Manzano, Alejandra
; Denova-Gutiérrez, Edgar
.
ABSTRACT OBJECTIVE To evaluate the association between dietary patterns, stunting, and overweight among Mexican preschoolers. METHODS This study was conducted with anthropometric (weight, height/length), sociodemographic (age, gender, education level of household head, socioeconomic status, country region and area, ethnicity, and beneficiary of social programs), and dietary data (Semiquantitative-food frequency questionnaire) on children aged from 1 to 4 years collected from the Mexican National Health and Nutrition Survey-2012. Dietary patterns were derived by principal components analysis. The association between dietary patterns, stunting, and overweight was assessed by prevalence ratios (PR), estimated by Poisson regression. RESULTS In total, 1,112 preschoolers (mean age 3.06 years, SD = 1.08 years; 48.8% females) were included in the study; 11.9% of whom presented stunting, and 6.7% overweight. We identified four dietary patterns: Fruits and Vegetables [F&V], Western [W], Traditional [T], and Milk and Liquids [M&L]. Considering the lowest tertile of each dietary pattern as reference, the prevalence of stunting was 2.04 times higher [95%CI: 1.17–3.56] among children in the highest tertile of the “F&V” pattern. The prevalence of stunting was lower among children in the highest tertile of the “W” pattern [PR = 0.48; 95%CI: 0.27–0.85]. Overweight was negatively associated with the “F&V” dietary pattern [PR = 0.37; 95%CI: 0.16–0.85 for its highest tertile], and children whose consumption was mostly equivalent to the “T” pattern showed higher prevalence of stunting [PR = 1.74; 95%CI: 1.01–3.00]. CONCLUSIONS The prevalence of stunting and overweight in a nationwide sample of Mexican preschoolers was associated with dietary patterns.
6.
Dietary patterns in Mexican preschool children are associated with stunting and overweight
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Flores, Mario E.
; Rivera-Pasquel, Marta
; Macías, Nayeli
; Sánchez-Zamorano, Luisa María
; Rodríguez-Ramírez, Sonia
; Contreras-Manzano, Alejandra
; Denova-Gutiérrez, Edgar
.
ABSTRACT OBJECTIVE To evaluate the association between dietary patterns, stunting, and overweight among Mexican preschoolers. METHODS This study was conducted with anthropometric (weight, height/length), sociodemographic (age, gender, education level of household head, socioeconomic status, country region and area, ethnicity, and beneficiary of social programs), and dietary data (Semiquantitative-food frequency questionnaire) on children aged from 1 to 4 years collected from the Mexican National Health and Nutrition Survey-2012. Dietary patterns were derived by principal components analysis. The association between dietary patterns, stunting, and overweight was assessed by prevalence ratios (PR), estimated by Poisson regression. RESULTS In total, 1,112 preschoolers (mean age 3.06 years, SD = 1.08 years; 48.8% females) were included in the study; 11.9% of whom presented stunting, and 6.7% overweight. We identified four dietary patterns: Fruits and Vegetables [F&V], Western [W], Traditional [T], and Milk and Liquids [M&L]. Considering the lowest tertile of each dietary pattern as reference, the prevalence of stunting was 2.04 times higher [95%CI: 1.17–3.56] among children in the highest tertile of the “F&V” pattern. The prevalence of stunting was lower among children in the highest tertile of the “W” pattern [PR = 0.48; 95%CI: 0.27–0.85]. Overweight was negatively associated with the “F&V” dietary pattern [PR = 0.37; 95%CI: 0.16–0.85 for its highest tertile], and children whose consumption was mostly equivalent to the “T” pattern showed higher prevalence of stunting [PR = 1.74; 95%CI: 1.01–3.00]. CONCLUSIONS The prevalence of stunting and overweight in a nationwide sample of Mexican preschoolers was associated with dietary patterns.
https://doi.org/10.11606/s1518-8787.2021055002350
62 downloads
7.
Consenso mexicano de linfoma de Hodgkin
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Álvarez-Vera, José L.
; Aguilar-Luevano, Jocelyn
; Alcívar-Cedeño, Luisa M.
; Arana-Luna, Luara L.
; Arteaga-Ortiz, Luis
; Báez-Islas, Pamela E.
; Carolina-Reynoso, Ana
; Cesarman-Maus, Gabriela
; Peña-Celaya, José A. De la
; Espitia-Ríos, María E.
; Fermín-Caminero, Denisse J.
; Flores-Patricio, Willy
; García-Camacho, Alinka S.
; Guzmán-Mora, Pedro G.
; Hernández-Colín, Ana K.
; Hernández-Ruiz, Eleazar
; Herrera-Olivares, Wilfrido
; Jacobo-Medrano, Elvia
; Loera-Fragoso, Sergio J.
; Macías-Flores, Juan P.
; Martínez-Ramírez, Mario A.
; Medina-Guzmán, Liliana
; Milán-Salvatierra, Andrea I.
; Montoya-Jiménez, Leire
; Morales-Adrián, Javier de J.
; Mujica-Martínez, Aldo
; Nava-Villegas, Lorena
; Orellana-Garibay, Juan J.
; Palma-Moreno, Orlando G.
; Pérez-Zúñiga, Juan M.
; Pérez-Gómez, Karen D.
; Pichardo-Cepín, Yayra M.
; Rojas-Castillejos, Flavio
; Romero-Martínez, Eduardo
; Romero-Rodelo, Hilda
; Segura-García, Adela
; Silva-Vera, Karina
; Tapía-Enríquez, Ana L.
; Teomitzi-Sánchez, Óscar
; Tepepa-Flores, Fredy
; Vilchis-González, Shendel P.
; Villela-Peña, Atenas
; Guerra-Alarcón, Lidia V.
; Reséndiz-Olea, Rodrigo
; Banda-García, Luisa
; Paredes-Lozano, Eugenia P.
; Alvarado-Ibarra, Martha
.
Abstract Hodgkin’s lymphoma is due to the clonal transformation of cells originating from B lymphocytes, generating the pathognomonic binucleate Reed-Sternberg cells. Hodgkin’s lymphoma is a B cell disease with a bimodal distribution, with higher incidence in adolescence and the third decade of life, showing a second peak in people over 55 years of age. Classic Hodgkin lymphoma cells routinely undergo gene expression reprogramming, as they lose the expression of most of the typical B-cell genes and acquire the expression of multiple genes that are typical of other types of cells in the immune system. The treatment algorithm will depend on whether it is classic or predominantly lymphocytic HL, if it is early stage with unfavorable prognostic markers or not, the initial management regimen, and whether there is bulky disease, among the most relevant variables.
Resumen El linfoma de Hodgkin (LH) se debe a la transformación clonal de células originadas en los linfocitos B, lo que genera las células binucleadas patognomónicas de Reed-Sternberg. El LH es una enfermedad de células B con una distribución bimodal, con mayor incidencia en la adolescencia y la tercera década de la vida y un segundo pico en personas mayores de 55 años. Las células del LH clásico habitualmente sufren una reprogramación de la expresión génica, ya que pierden la expresión de la mayoría de los genes típicos de las células B y han adquirido la expresión de múltiples genes que son típicos de otros tipos de células del sistema inmunitario. El algoritmo de tratamiento dependerá si se trata de LH clásico o de predominio linfocítico, si es un estadio temprano con marcadores de pronóstico desfavorables o no, el esquema inicial de manejo y si existe enfermedad voluminosa, entre las variables más relevantes.
https://doi.org/10.24875/gmm.m21000500
304 downloads
8.
Consenso de hemofilia en México
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López-Arroyo, José L.
; Pérez-Zúñiga, Juan M.
; Merino-Pasaye, Laura E.
; Saavedra-González, Azucena
; Alcivar-Cedeño, Luisa María
; Álvarez-Vera, José Luis
; Anaya-Cuellar, Irene
; Arana-Luna, Luara L.
; Ávila-Castro, David
; Bates-Martín, Ramón A.
; Cesarman-Maus, Gabriela
; Chávez-Aguilar, Lénica A.
; Peña-Celaya, José A. de la
; Espitia-Ríos, María E.
; Estrada-Domínguez, Patricia
; Fermín-Caminero, Denisse
; Flores-Patricio, Willy
; García Chávez, Jaime
; García-Lee, María T.
; González-Pérez, María del Carmen
; González-Rubio, María del Carmen
; González-Villareal, María Guadalupe
; Ramírez-Moreno, Fabiola
; Hernández-Colin, Ana K.
; Hernández-Ruiz, Eleazar
; Herrera-Olivares, Wilfrido
; Leyto-Cruz, Faustino
; Loera-Fragoso, Sergio
; Martínez-Ríos, Annel
; Miranda-Madrazo, María R.
; Morales-Hernández, Alba
; Nava-Villegas, Lorena
; Orellana-Garibay, Juan J.
; Palma-Moreno, Orlando G.
; Paredes-Lozano, Eugenia P.
; Peña-Alcántara, Paula
; Pérez-Lozano, Uendy
; Pichardo-Cepín, Yayra M.
; Reynoso-Pérez, Ana Carolina
; Rodríguez-Serna, Mishel
; Rojas-Castillejos, Flavio
; Romero-Rodelo, Hilda
; Ruíz-Contreras, Josué I.
; Segura-García, Adela
; Silva-Vera, Karina
; Soto-Cisneros, Paulina M.
; Tapia-Enríquez, Ana L.
; Tavera-Rodríguez, Martha G.
; Teomitzi-Sánchez, Óscar
; Tepepa-Flores, Fredy
; Valencia-Rivas, María D.
; Valle-Cárdenas, Teresa
; Varela-Constantino, Ana
; Javier-Morales, Adrián
; Martínez-Ramírez, Mario A.
; Tena-Cano, Sergio
; Terrazas-Marín, Ricardo
; Vilchis-González, Shendel P.
; Villela-Peña, Atenas
; Mena-Zepeda, Verónica
; Alvarado Ibarra, Martha
.
Abstract Hemophilia is a hemorrhagic disorder with a sex-linked inherited pattern, characterized by an inability to amplify coagulation due to a deficiency in coagulation factor VIII (hemophilia A or classic) or factor IX (hemophilia B). Sequencing of the genes involved in hemophilia has provided a description and record of the main mutations, as well as a correlation with the various degrees of severity. Hemorrhagic manifestations are related to levels of circulating factor, mainly affecting the musculoskeletal system and specifically the large joints (knees, ankles and elbows). This document is a review and consensus of the main genetic aspects of hemophilia, from the inheritance pattern to the concept of women carriers, physiopathology and classification of the disorder, the basic and confirmation studies when hemophilia is suspected, the various treatment regimens based on infusion of the deficient coagulation factor as well as innovative factor-free therapies and recommendations for the management of complications associated with treatment (development of inhibitors and/or transfusion transmitted infections) or secondary to articular hemorrhagic events (hemophilic arthropathy). Finally, relevant reviews of clinical and treatment aspects of hemorrhagic pathology charachterized by acquired deficiency of FVIII secondary to neutralized antibodies named acquired hemophilia.
resumen está disponible en el texto completo
https://doi.org/10.24875/gmm.m20000451
305 downloads
9.
Diagnóstico y tratamiento de la etapa aguda del accidente cerebrovascular isquémico: Guía de práctica clínica del Seguro Social del Perú (EsSalud)
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Sequeiros-Chirinos, Joel Mario
; Alva-Díaz, Carlos A.
; Pacheco-Barrios, Kevin
; Huaringa-Marcelo, Jorge
; Huamaní, Charles
; Camarena-Flores, Carla E.
; Durand-Castro, Walter S.
; Valencia-Chávez, Ana María
; Ecos-Quispe, Rosa L.
; Estupinan-Valdez, Paula I.
; Gallo-Guerrero, Marla
; Huamaní-Mendoza, Marlene Doris
; Mariños-Sánchez, Evelyn Vanessa
; Morón-Cabrera, María Elizabeth
; Pulachet-Contreras, Elena
; Ramos-Auccasi, Alfredo
; Rodríguez-Kadota, Liliana
; Saavedra-Rocha, César
; Chávez-Rimache, Lesly
; Timaná-Ruiz, Raúl
.
ABSTRACT Objective: to provide evidence based clinical recommendations for the diagnosis and therapy of the acute stage of ischemic cerebrovascular accident in EsSalud. Materials and methods : a group for producing the guideline was conveyed, including specialized physicians and methodologists. The group prepared 8 clinical questions to be answered by the guideline. Systematic searches of previous reviews were performed, and - when it was deemed necessary - primary studies in Medline and the Cochrane Controlled Registry of Trials for 2018 were reviewed. Evidence was selected aiming to respond each one of the proposed clinical questions. Certainty of the evidence was determined using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) methodology. In periodic work sessions, the group used GRADE methodology for reviewing the evidence and generated recommendations, good clinical practice items, and designed flow charts for both diagnosis and therapy. Finally, the guide was approved with the decree N° 128-IETSI-ESSALUD-2019. Results : this clinical practice guideline focused in 8 clinical questions, which were divided into four topics: screening, diagnosis, therapy, support and rehabilitation. On the basis of these questions, 28 recommendations were formulated (8 strong and 20 conditional), 38 items for good clinical practice, 1 implementation note, and 2 flow charts. Conclusion : this paper summarizes both evidence based methodology and conclusions from a new clinical practice guide for diagnosis and therapy of the acute stage of a cerebrovascular accident in EsSalud.
RESUMEN Objetivo: proveer recomendaciones clínicas basadas en evidencia para el diagnóstico y tratamiento de la etapa aguda del ataque cerebrovascular isquémico en EsSalud. Materiales y métodos: se conformó un grupo elaborador de la guía (GEG) que incluyó médicos especialistas y metodólogos. El GEG formuló 8 preguntas clínicas a ser respondidas por la presente GPC. Se realizó búsquedas sistemáticas de revisiones sistemáticas y -cuando fue considerado pertinente- estudios primarios en Medline y Cochrane Controlled Register of Trials durante el 2018. Se seleccionó la evidencia para responder cada una de las preguntas clínicas planteadas. La certeza de la evidencia fue evaluada usando la metodología Grading of Recommendations Assessment, Development, and Evaluation (GRADE). En reuniones de trabajo periódicas, el GEG usó la metodología GRADE para revisar la evidencia y formular las recomendaciones, los puntos de buenas prácticas clínicas y el flujograma de diagnóstico y tratamiento. Finalmente, la GPC fue aprobada con Resolución N° 128-IETSI-ESSALUD-2019. Resultados: la presente GPC abordó 8 preguntas clínicas, divididas en cuatro temas: tamizaje, diagnóstico, tratamiento, soporte y rehabilitación. En base a estas preguntas se formularon 28 recomendaciones (8 fuertes y 20 condicionales), 38 puntos de buena práctica clínica, 1 nota de implementación y 2 flujogramas. Conclusión: el presente artículo resume la metodología y las conclusiones basadas en evidencias de la GPC para el diagnóstico y tratamiento de la etapa aguda del ataque cerebrovascular isquémico en EsSalud.
10.
Diversidad del paisaje y remoción del estiércol por escarabajos coprófagos en pastizales del norte de Veracruz
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Miranda-Flores, Karen Pamela
; Chamorro-Florescano, Ivette Alicia
; Favila, Mario E.
; Alanís-Méndez, José Luis
; Ortiz-Domínguez, Maribel
.
Abstract We analyzed the composition of species and evaluated the efficiency in the removal of cow dung by dung beetles (Scarabaeinae) in 2 livestock areas of northern Veracruz (Tamiahua and Tlacolula) with different landscape during the rainy and dry seasons. Tamiahua, with grasslands without tree vegetation, only with isolated trees, and Tlacolula, with tree vegetation around the grasslands and live fences. Five species were collected in Tamiahua and 16 in Tlacolula. The most abundant species in Tamiahua were: Canthidium pseudopuncticolle Harold, Canthon cyanellus cyanellus LeConte, and Digitonthophagus gazella Fabricius, in Tlacolula: C. c. cyanellus, Coprophanaeus pluto Harold, and C. pseudopuncticolle. During the rainy season, the removal of dung was greater in the Tlacolula grasslands than in the Tamiahua grasslands; however, in both locations, there was no removal of excrement during the dry season. In both areas, the dominant species in the dung removal process was D. gazella, an African introduced species. Our results suggest that the reincorporation of manure is affected by the species composition, the time of year and that heterogeneous environments favor the diversity of dung beetle species in livestock areas of northern Veracruz.
Resumen Se analizó la composición de especies y se evaluó la eficiencia en la remoción de estiércol vacuno por escarabajos del estiércol de la subfamilia Scarabaeinae durante las temporadas de lluvias y secas en 2 zonas ganaderas del norte de Veracruz con diferente cobertura vegetal. Tamiahua, con pastizales sin vegetación arbórea, solo con árboles aislados y Tlacolula, con vegetación arbórea en los alrededores de los pastizales y cercas vivas. Se recolectaron 5 especies en Tamiahua y 16 en Tlacolula. Las especies más abundantes en Tamiahua fueron: Canthidium pseudopuncticolle (Harold, 1867), Canthon cyanellus cyanellus LeConte y Digitonthophagus gazella Fabricius, mientras que en Tlacolula fueron: C. c. cyanellus, Coprophanaeus pluto Harold y C. pseudopuncticolle. En la temporada de lluvias, la remoción del estiércol fue mayor en los pastizales de Tlacolula que en los de Tamiahua; sin embargo, en ambas localidades no hubo remoción de estiércol en la temporada de secas. En ambas zonas, la especie dominante en el proceso de remoción de estiércol fue D. gazella, especie africana introducida. Nuestros resultados sugieren que la reincorporación del estiércol se ve afectada por la composición de especies, la temporada del año y por la heterogeneidad del paisaje en las zonas ganaderas del norte de Veracruz.
https://doi.org/10.22201/ib.20078706e.2020.91.2792
352 downloads
11.
Consenso Mexicano de Mieloma Múltiple
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Peña-Celaya, José A. de la
; Aguilar-Luevano, Jocelyn
; Alcivar-Cedeño, Luisa María
; Álvarez-Vera, José L.
; Anaya-Cuellar, Irene
; Añorve-Hernández, Erika
; Arana-Luna, Luara L.
; Arteaga-Ortíz, Luis
; Báez-Islas, Pamela E.
; Banda-García, Luisa I.
; Bates-Martín, Ramón A.
; Campa-Monroy, Dafne Itzel
; Cardiel-Silva, Mariela
; Castillo-Salas, Ángel de Jesús
; Cota-Rangel, Xóchitl
; Díaz-Vargas, Guillermo
; Espitia-Ríos, M. Eugenia
; Estrada-Domínguez, Patricia
; Fermín-Caminero, Denisse
; García-Camacho, Alinka
; García-Castillo, Carolina
; Garzón-Velásquez, Katheryn B.
; Gil-Rondero, Carlos
; Hernández-Colín, Ana K.
; Hernández-Ruiz, Eleazar
; Hernández-Alcántara, Areli E.
; Hernández-Cervantes, Silvia A.
; Herrera-Olivares, Wilfrido
; Ignacio-Ibarra, Gregorio
; Inclán-Alarcón, Sergio I.
; Leyto-Cruz, Faustino
; Macías-Flores, Juan P.
; Martínez-de la Vega, Andrea
; Martínez-Ramírez, Mario A.
; Martínez-Coronel, Jorge
; Medina-Coral, Jesús E.
; Meza-Dávalos, Lizeth
; Montoya-Jiménez, Leire
; Morales-Hernández, Alba
; Morales-López, Elizabeth
; Morales-Adrián, Javier de Jesús
; Morales-de Azcué, Maricruz
; Mújica-Martínez, Aldo
; Murillo-Cruz, Juan L.
; Nájera-Martínez, Jéssica
; Narváez-Sarmiento, Iris M.
; Nava-Villegas, Lorena
; Nava-Alpide, Marco A.
; Orellana-Garibay, Juan J.
; Palafox-Zaldívar, María Teresa
; Palma-Moreno, Orlando G.
; Paredes-Lozano, Eugenia P.
; Pedraza-Colín, María Luisa
; Pérez-Zúñiga, Juan M.
; Pérez-Lizardi, Alejandra B.
; Rojas-Castillejos, Flavio
; Romero-Martínez, Eduardo
; Romero-Rodelo, Hilda
; Ruiz-Contreras, Josué
; Saavedra-González, Azucena
; Saucedo-Montes, Erick
; Silva-Michel, Luis G.
; Silva-Vera, Karina
; Teomitzi-Sánchez, Óscar
; Tepepa-Flores, Fredy
; Ventura-Enríquez, Yanet
; Villela-Peña, Atenas
; Vilchis-González, Shendel P.
; Zapata-Canto, Nidia
; Zárate-Rodríguez, Pedro A.
; Alvarado-Ibarra, Martha
.
Abstract To identify this increasingly common pathology, known as Multiple Myeloma, it is necessary to refer to the specific factors that characterize it; to this end, the classic criteria known as CRAB (hypercalcemia, renal failure, anemia and lytic lesions) are available, in which renal failure is one of the most frequent complications. Recently, 3 indisputable biomarkers have been described for the diagnostic support for Multiple Myeloma, which are: more than 10% of clonal plasma cells in BM or, a biopsy that corroborates the presence of a plasmacytoma, light chain ratio ≥100 mg/dL and more than one focal lesion on magnetic resonance imaging (MRI). A differential diagnosis for plasma cell leukemia, solitary bone plasmacytoma, and extramedullary plasmacytoma should always be considered. Being this an incurable disease, a lot of research has been done regarding its therapeutic management, whose main objective is the disappearance of plasma cells and the patient clinical improvement. Melphalan was the first drug that showed a benefit in 1958 and afterwards, with the addition of a steroid as a second drug, it was possible to improve response rates. Subsequently, different molecules were studied, forming multiple combinations, and achieving better rates of Overall Survival and Progression-Free Survival. Years later, with the arrival of proteasome inhibitors such as bortezomib, and immunomodulators such as thalidomide and lenalidomide, an important turnaround in the disease has been seen, as deeper responses, more prolonged remissions, and improvement in the quality of life of patients have been achieved. This consensus has the purpose of integrating a group of Mexican specialists and promoting the updating of this pathology.
resumen está disponible en el texto completo
https://doi.org/10.24875/gmm.m20000392
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12.
System Modeling for Priority Schemes in Managed Peer-to-Peer Networks
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Abstract: In Peer-to-Peer (P2P) networks it is not uncommon to find users that have initiated a file transfer and decide to leave the system before the end of the download. This is a particularly harmful behavior due to the resources (bandwidth or energy) wasted in such an aborted process. This negative effect is amplified when the system’s conditions are such that the peers are not downloading at the maximum capacity. This is because in these conditions, there are not enough peers uploading the file, and a part of the bandwidth is wasted on peers that do not share their resources to the network once they leave the system. In this paper, the case of managed P2P networks is of special interest. As such, the variability in the transfer rates, such as the download and upload rates, is expected to be much less than in the case of the unmanaged P2P network. The limit case, where these transfer rates are constant is considered as an alternative to the exponential distribution. Additionally, two priority schemes are presented for a BitTorrent-based P2P network where the peers that are more likely to remain longer in the system are served first.
https://doi.org/10.13053/cys-22-2-2747
67 downloads
13.
Growing knowledge: an overview of Seed Plant diversity in Brazil
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Zappi, Daniela C.
; Filardi, Fabiana L. Ranzato
; Leitman, Paula
; Souza, Vinícius C.
; Walter, Bruno M.T.
; Pirani, José R.
; Morim, Marli P.
; Queiroz, Luciano P.
; Cavalcanti, Taciana B.
; Mansano, Vidal F.
; Forzza, Rafaela C.
; Abreu, Maria C.
; Acevedo-Rodríguez, Pedro
; Agra, Maria F.
; Almeida Jr., Eduardo B.
; Almeida, Gracineide S.S.
; Almeida, Rafael F.
; Alves, Flávio M.
; Alves, Marccus
; Alves-Araujo, Anderson
; Amaral, Maria C.E.
; Amorim, André M.
; Amorim, Bruno
; Andrade, Ivanilza M.
; Andreata, Regina H.P.
; Andrino, Caroline O.
; Anunciação, Elisete A.
; Aona, Lidyanne Y.S.
; Aranguren, Yani
; Aranha Filho, João L.M.
; Araújo, Andrea O.
; Araújo, Ariclenes A.M.
; Araújo, Diogo
; Arbo, María M.
; Assis, Leandro
; Assis, Marta C.
; Assunção, Vivian A.
; Athiê-Souza, Sarah M.
; Azevedo, Cecilia O.
; Baitello, João B.
; Barberena, Felipe F.V.A.
; Barbosa, Maria R.V.
; Barros, Fábio
; Barros, Lucas A.V.
; Barros, Michel J.F.
; Baumgratz, José F.A.
; Bernacci, Luis C.
; Berry, Paul E.
; Bigio, Narcísio C.
; Biral, Leonardo
; Bittrich, Volker
; Borges, Rafael A.X.
; Bortoluzzi, Roseli L.C.
; Bove, Cláudia P.
; Bovini, Massimo G.
; Braga, João M.A.
; Braz, Denise M.
; Bringel Jr., João B.A.
; Bruniera, Carla P.
; Buturi, Camila V.
; Cabral, Elza
; Cabral, Fernanda N.
; Caddah, Mayara K.
; Caires, Claudenir S.
; Calazans, Luana S.B.
; Calió, Maria F.
; Camargo, Rodrigo A.
; Campbell, Lisa
; Canto-Dorow, Thais S.
; Carauta, Jorge P.P.
; Cardiel, José M.
; Cardoso, Domingos B.O.S.
; Cardoso, Leandro J.T.
; Carneiro, Camila R.
; Carneiro, Cláudia E.
; Carneiro-Torres, Daniela S.
; Carrijo, Tatiana T.
; Caruzo, Maria B.R.
; Carvalho, Maria L.S.
; Carvalho-Silva, Micheline
; Castello, Ana C.D.
; Cavalheiro, Larissa
; Cervi, Armando C.
; Chacon, Roberta G.
; Chautems, Alain
; Chiavegatto, Berenice
; Chukr, Nádia S.
; Coelho, Alexa A.O.P.
; Coelho, Marcus A.N.
; Coelho, Rubens L.G.
; Cordeiro, Inês
; Cordula, Elizabeth
; Cornejo, Xavier
; Côrtes, Ana L.A.
; Costa, Andrea F.
; Costa, Fabiane N.
; Costa, Jorge A.S.
; Costa, Leila C.
; Costa-e-Silva, Maria B.
; Costa-Lima, James L.
; Cota, Maria R.C.
; Couto, Ricardo S.
; Daly, Douglas C.
; De Stefano, Rodrigo D.
; De Toni, Karen
; Dematteis, Massimiliano
; Dettke, Greta A.
; Di Maio, Fernando R.
; Dórea, Marcos C.
; Duarte, Marília C.
; Dutilh, Julie H.A.
; Dutra, Valquíria F.
; Echternacht, Lívia
; Eggers, Lilian
; Esteves, Gerleni
; Ezcurra, Cecilia
; Falcão Junior, Marcus J.A.
; Feres, Fabíola
; Fernandes, José M.
; Ferreira, D.M.C.
; Ferreira, Fabrício M.
; Ferreira, Gabriel E.
; Ferreira, Priscila P.A.
; Ferreira, Silvana C.
; Ferrucci, Maria S.
; Fiaschi, Pedro
; Filgueiras, Tarciso S.
; Firens, Marcela
; Flores, Andreia S.
; Forero, Enrique
; Forster, Wellington
; Fortuna-Perez, Ana P.
; Fortunato, Reneé H.
; Fraga, Cléudio N.
; França, Flávio
; Francener, Augusto
; Freitas, Joelcio
; Freitas, Maria F.
; Fritsch, Peter W.
; Furtado, Samyra G.
; Gaglioti, André L.
; Garcia, Flávia C.P.
; Germano Filho, Pedro
; Giacomin, Leandro
; Gil, André S.B.
; Giulietti, Ana M.
; A.P.Godoy, Silvana
; Goldenberg, Renato
; Gomes da Costa, Géssica A.
; Gomes, Mário
; Gomes-Klein, Vera L.
; Gonçalves, Eduardo Gomes
; Graham, Shirley
; Groppo, Milton
; Guedes, Juliana S.
; Guimarães, Leonardo R.S.
; Guimarães, Paulo J.F.
; Guimarães, Elsie F.
; Gutierrez, Raul
; Harley, Raymond
; Hassemer, Gustavo
; Hattori, Eric K.O.
; Hefler, Sonia M.
; Heiden, Gustavo
; Henderson, Andrew
; Hensold, Nancy
; Hiepko, Paul
; Holanda, Ana S.S.
; Iganci, João R.V.
; Imig, Daniela C.
; Indriunas, Alexandre
; Jacques, Eliane L.
; Jardim, Jomar G.
; Kamer, Hiltje M.
; Kameyama, Cíntia
; Kinoshita, Luiza S.
; Kirizawa, Mizué
; Klitgaard, Bente B.
; Koch, Ingrid
; Koschnitzke, Cristiana
; Krauss, Nathália P.
; Kriebel, Ricardo
; Kuntz, Juliana
; Larocca, João
; Leal, Eduardo S.
; Lewis, Gwilym P.
; Lima, Carla T.
; Lima, Haroldo C.
; Lima, Itamar B.
; Lima, Laíce F.G.
; Lima, Laura C.P.
; Lima, Leticia R.
; Lima, Luís F.P.
; Lima, Rita B.
; Lírio, Elton J.
; Liro, Renata M.
; Lleras, Eduardo
; Lobão, Adriana
; Loeuille, Benoit
; Lohmann, Lúcia G.
; Loiola, Maria I.B.
; Lombardi, Julio A.
; Longhi-Wagner, Hilda M.
; Lopes, Rosana C.
; Lorencini, Tiago S.
; Louzada, Rafael B.
; Lovo, Juliana
; Lozano, Eduardo D.
; Lucas, Eve
; Ludtke, Raquel
; Luz, Christian L.
; Maas, Paul
; Machado, Anderson F.P.
; Macias, Leila
; Maciel, Jefferson R.
; Magenta, Mara A.G.
; Mamede, Maria C.H.
; Manoel, Evelin A.
; Marchioretto, Maria S.
; Marques, Juliana S.
; Marquete, Nilda
; Marquete, Ronaldo
; Martinelli, Gustavo
; Martins da Silva, Regina C.V.
; Martins, Ângela B.
; Martins, Erika R.
; Martins, Márcio L.L.
; Martins, Milena V.
; Martins, Renata C.
; Matias, Ligia Q.
; Maya-L., Carlos A.
; Mayo, Simon
; Mazine, Fiorella
; Medeiros, Debora
; Medeiros, Erika S.
; Medeiros, Herison
; Medeiros, João D.
; Meireles, José E.
; Mello-Silva, Renato
; Melo, Aline
; Melo, André L.
; Melo, Efigênia
; Melo, José I.M.
; Menezes, Cristine G.
; Menini Neto, Luiz
; Mentz, Lilian A.
; Mezzonato, A.C.
; Michelangeli, Fabián A.
; Milward-de-Azevedo, Michaele A.
; Miotto, Silvia T.S.
; Miranda, Vitor F.O.
; Mondin, Cláudio A.
; Monge, Marcelo
; Monteiro, Daniele
; Monteiro, Raquel F.
; Moraes, Marta D.
; Moraes, Pedro L.R.
; Mori, Scott A.
; Mota, Aline C.
; Mota, Nara F.O.
; Moura, Tania M.
; Mulgura, Maria
; Nakajima, Jimi N.
; Nardy, Camila
; Nascimento Júnior, José E.
; Noblick, Larry
; Nunes, Teonildes S.
; O'Leary, Nataly
; Oliveira, Arline S.
; Oliveira, Caetano T.
; Oliveira, Juliana A.
; Oliveira, Luciana S.D.
; Oliveira, Maria L.A.A.
; Oliveira, Regina C.
; Oliveira, Renata S.
; Oliveira, Reyjane P.
; Paixão-Souza, Bruno
; Parra, Lara R.
; Pasini, Eduardo
; Pastore, José F.B.
; Pastore, Mayara
; Paula-Souza, Juliana
; Pederneiras, Leandro C.
; Peixoto, Ariane L.
; Pelissari, Gisela
; Pellegrini, Marco O.O.
; Pennington, Toby
; Perdiz, Ricardo O.
; Pereira, Anna C.M.
; Pereira, Maria S.
; Pereira, Rodrigo A.S.
; Pessoa, Clenia
; Pessoa, Edlley M.
; Pessoa, Maria C.R.
; Pinto, Luiz J.S.
; Pinto, Rafael B.
; Pontes, Tiago A.
; Prance, Ghillean T.
; Proença, Carolyn
; Profice, Sheila R.
; Pscheidt, Allan C.
; Queiroz, George A.
; Queiroz, Rubens T.
; Quinet, Alexandre
; Rainer, Heimo
; Ramos, Eliana
; Rando, Juliana G.
; Rapini, Alessandro
; Reginato, Marcelo
; Reis, Ilka P.
; Reis, Priscila A.
; Ribeiro, André R.O.
; Ribeiro, José E.L.S.
; Riina, Ricarda
; Ritter, Mara R.
; Rivadavia, Fernando
; Rocha, Antônio E.S.
; Rocha, Maria J.R.
; Rodrigues, Izabella M.C.
; Rodrigues, Karina F.
; Rodrigues, Rodrigo S.
; Rodrigues, Rodrigo S.
; Rodrigues, Vinícius T.
; Rodrigues, William
; Romaniuc Neto, Sérgio
; Romão, Gerson O.
; Romero, Rosana
; Roque, Nádia
; Rosa, Patrícia
; Rossi, Lúcia
; Sá, Cyl F.C.
; Saavedra, Mariana M.
; Saka, Mariana
; Sakuragui, Cássia M.
; Salas, Roberto M.
; Sales, Margareth F.
; Salimena, Fatima R.G.
; Sampaio, Daniela
; Sancho, Gisela
; Sano, Paulo T.
; Santos, Alessandra
; Santos, Élide P.
; Santos, Juliana S.
; Santos, Marianna R.
; Santos-Gonçalves, Ana P.
; Santos-Silva, Fernanda
; São-Mateus, Wallace
; Saraiva, Deisy P.
; Saridakis, Dennis P.
; Sartori, Ângela L.B.
; Scalon, Viviane R.
; Schneider, Ângelo
; Sebastiani, Renata
; Secco, Ricardo S.
; Senna, Luisa
; Senna-Valle, Luci
; Shirasuna, Regina T.
; Silva Filho, Pedro J.S.
; Silva, Anádria S.
; Silva, Christian
; Silva, Genilson A.R.
; Silva, Gisele O.
; Silva, Márcia C.R.
; Silva, Marcos J.
; Silva, Marcos J.
; Silva, Otávio L.M.
; Silva, Rafaela A.P.
; Silva, Saura R.
; Silva, Tania R.S.
; Silva-Gonçalves, Kelly C.
; Silva-Luz, Cíntia L.
; Simão-Bianchini, Rosângela
; Simões, André O.
; Simpson, Beryl
; Siniscalchi, Carolina M.
; Siqueira Filho, José A.
; Siqueira, Carlos E.
; Siqueira, Josafá C.
; Smith, Nathan P.
; Snak, Cristiane
; Soares Neto, Raimundo L.
; Soares, Kelen P.
; Soares, Marcos V.B.
; Soares, Maria L.
; Soares, Polyana N.
; Sobral, Marcos
; Sodré, Rodolfo C.
; Somner, Genise V.
; Sothers, Cynthia A.
; Sousa, Danilo J.L.
; Souza, Elnatan B.
; Souza, Élvia R.
; Souza, Marcelo
; Souza, Maria L.D.R.
; Souza-Buturi, Fátima O.
; Spina, Andréa P.
; Stapf, María N.S.
; Stefano, Marina V.
; Stehmann, João R.
; Steinmann, Victor
; Takeuchi, Cátia
; Taylor, Charlotte M.
; Taylor, Nigel P.
; Teles, Aristônio M.
; Temponi, Lívia G.
; Terra-Araujo, Mário H.
; Thode, Veronica
; Thomas, W.Wayt
; Tissot-Squalli, Mara L.
; Torke, Benjamin M.
; Torres, Roseli B.
; Tozzi, Ana M.G.A.
; Trad, Rafaela J.
; Trevisan, Rafael
; Trovó, Marcelo
; Valls, José F.M.
; Vaz, Angela M.S.F.
; Versieux, Leonardo
; Viana, Pedro L.
; Vianna Filho, Marcelo D.M.
; Vieira, Ana O.S.
; Vieira, Diego D.
; Vignoli-Silva, Márcia
; Vilar, Thaisa
; Vinhos, Franklin
; Wallnöfer, Bruno
; Wanderley, Maria G.L.
; Wasshausen, Dieter
; Watanabe, Maurício T.C.
; Weigend, Maximilian
; Welker, Cassiano A.D.
; Woodgyer, Elizabeth
; Xifreda, Cecilia C.
; Yamamoto, Kikyo
; Zanin, Ana
; Zenni, Rafael D.
; Zickel, Carmem S
.
Resumo Um levantamento atualizado das plantas com sementes e análises relevantes acerca desta biodiversidade são apresentados. Este trabalho se iniciou em 2010 com a publicação do Catálogo de Plantas e Fungos e, desde então vem sendo atualizado por mais de 430 especialistas trabalhando online. O Brasil abriga atualmente 32.086 espécies nativas de Angiospermas e 23 espécies nativas de Gimnospermas e estes novos dados mostram um aumento de 3% da riqueza em relação a 2010. A Amazônia é o Domínio Fitogeográfico com o maior número de espécies de Gimnospermas, enquanto que a Floresta Atlântica possui a maior riqueza de Angiospermas. Houve um crescimento considerável no número de espécies e nas taxas de endemismo para a maioria dos Domínios (Caatinga, Cerrado, Floresta Atlântica, Pampa e Pantanal), com exceção da Amazônia que apresentou uma diminuição de 2,5% de endemicidade. Entretanto, a maior parte das plantas com sementes que ocorrem no Brasil (57,4%) é endêmica deste território. A proporção de formas de vida varia de acordo com os diferentes Domínios: árvores são mais expressivas na Amazônia e Floresta Atlântica do que nos outros biomas, ervas são dominantes no Pampa e as lianas apresentam riqueza expressiva na Amazônia, Floresta Atlântica e Pantanal. Este trabalho não só quantifica a biodiversidade brasileira, mas também indica as lacunas de conhecimento e o desafio a ser enfrentado para a conservação desta flora.
Abstract An updated inventory of Brazilian seed plants is presented and offers important insights into the country's biodiversity. This work started in 2010, with the publication of the Plants and Fungi Catalogue, and has been updated since by more than 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing an increase of 3% in its species richness in relation to 2010. The Amazon Rainforest is the richest Brazilian biome for Gymnosperms, while the Atlantic Rainforest is the richest one for Angiosperms. There was a considerable increment in the number of species and endemism rates for biomes, except for the Amazon that showed a decrease of 2.5% of recorded endemics. However, well over half of Brazillian seed plant species (57.4%) is endemic to this territory. The proportion of life-forms varies among different biomes: trees are more expressive in the Amazon and Atlantic Rainforest biomes while herbs predominate in the Pampa, and lianas are more expressive in the Amazon, Atlantic Rainforest, and Pantanal. This compilation serves not only to quantify Brazilian biodiversity, but also to highlight areas where there information is lacking and to provide a framework for the challenge faced in conserving Brazil's unique and diverse flora.
https://doi.org/10.1590/2175-7860201566411
33340 downloads
14.
First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina
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Carbonari, Claudia C
; Deza, Natalia
; Flores, Mario
; Gasparini, Alejandra
; Manfredi, Eduardo
; Rivas, Marta
.
We describe the first isolation of an enteroaggregative Escherichia coli (EAEC) O104:H4 strain associated with an acute diarrhea case in Argentina. Two multiplex PCRs (mPCR) were performed as screening of genes mPCR1 (eae, lt, and st) and mPCR2 (IpaH, aggR, stx1 and stx2). A mPCR to detect the rfbO104, fliC H4 and terD genes, and PCR assays for the detection of pCVD432 plasmid, aaiC and lpfO113 genes were included. Biochemical and antimicrobial susceptibility assays as well as serotyping were performed. The identified E. coli strain was susceptible to all antimicrobials tested and harbored the aggR, aaiC, pCVD432 plasmid, lpfO113, rfbO104, fliC H4 and terD genes. Although serotype EAEC O104:H4 rarely spreads and sporadic cases have been reported, global concern increased after the large-scale outbreak in Europe in 2011. The finding of EAEC O104:H4 reinforces the need for improved methodologies for the detection of all E. coli pathotypes.
Se describe el primer aislamiento de una cepa de Escherichia coli enteroagregativo (EAEC) O104:H4 de un caso de diarrea aguda en Argentina. Se realizaron dos PCR múltiples como tamizaje: mPCR1 para los genes eae, lt y st, y mPCR2 para los genes IpaH, aggR, stx1y stx2. Se incluyó una mPCR para detectar los genes rfbO104, fliC H4 y terD, además de PCR simples para los genes del plásmido pCVD432, aaiC y lpfO113. Se realizaron ensayos bioquímicos, de sensibilidad a los antimicrobianos y de serotipificación. La cepa de E. coli identificada fue sensible a todos los antimicrobianos ensayados y presentó los genes aggR, aaiC, plásmido pCVD432, lpfO113, rfbO104, fliC H4 y terD. Si bien EAEC O104:H4 es un serotipo poco común, se han comunicado casos esporádicos, pero la preocupación global aumentó después del brote masivo ocurrido en Europa en 2011. El hallazgo de EAEC O104:H4 refuerza la necesidad de mejorar las metodologías para la detección de todos los patotipos de E. coli en Argentina.
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15.
Adhesive and invasive capacities of Edwarsiella tarda isolated from South American sea lion
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Fernández, Araceli
; Paz Villanueva, María
; González, Mario
; Fernández, Fabiola
; Latif, Fadua
; Flores, Sandra Nonier
; Fernández, Heriberto
.
Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and invasive ability of 17 E. tarda strains isolated from SASL fecal material. All the strains were able to adhere and invade HEp-2 cells with adhesion and invasion percentages ranging from 56 to 100% and 21 to 74%, respectively. Despite the expression of these pathogenic factors, further investigation is needed to determine whether this bacterium could play a role as primary pathogen for this and other species of pinnipeds.
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