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Crotalaria ochroleuca (Fabaceae) is rich in bioactive compounds used for nematode control. Transformed root culture allows the production of a large amount of hairy root biomass, conditioned by auxin biosynthesis-related genes integrated via the process of Agrobacterium rhizogenes infection, which is known to cause a hairy root phenotype. We transformed A. rhizogenes to increase the biomass of hairy roots and optimized the process of production of bioactive compounds. The transformed nature of hairy roots was confirmed by polymerase chain reaction, which confers kanamycin resistance. The transformed roots were cultured in full-strength in liquid media to realize the growth of hairy roots and the production of bioactive compounds. The transformed roots were grown in a culture medium supplemented with elicitors to produce total phenols, and flavonoids. Chromatographic analysis of transformed roots revealed the presence of flavonoids apigenin-6,8-C-diglucoside and luteolin-6-C-glucoside. The results were obtained by conducting physiological and biochemical studies with the Flavonoids and studying the pathways that led to the production of large amounts of bioactive compounds from the hairy roots of C. ochroleuca. It was observed that the extraction of the compounds significantly affected nematodes and insect larvae, resulting in significantly high levels of economic damage to crops.

https://doi.org/10.21577/0103-5053.20230083

Leaf extract of Coffea arabica L. reduces lipid peroxidation and has anti-platelet effect in a rat dyslipidemia model
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Simões, Mario Henrique Souza

; Salles, Bruno Cesar Correa

; Duarte, Stella Maris da Silveira

; Silva, Marcelo Aparecido da

; Viana, André Luiz Machado

; Moraes, Gabriel de Oliveira Isaac de

; Figueiredo, Sonia Aparecida

; Ferreira, Eric Batista

; Rodrigues, Maria Rita

; Paula, Fernanda Borges de Araújo

Brazilian Journal of Pharmaceutical Sciences

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2022, Volume 58 elocation e19562

Abstract: En | Text: En | PDF: En

Abstract This study aimed to evaluate the antioxidant potential of the Coffea arabica Lineu (L.) leaf extract and its effects on platelet aggregation of dyslipidemic rats. The extract was obtained by the percolation of C. arabica L. leaves in hydroethanolic solution 70% (v/v). The mass spectrometry FIA-ESI-MS² suggested the presence of chlorogenic acid, rutin acid, and quinic acid. The DPPH• radicals scavenging capacity was demonstrated (IC50 = 0.06 mg/mL). The extract was administered to rats by gavage (300 mg/kg/day) for 56 days. Dyslipidemia was induced by administering Triton WR-1339 (300 mg/kg body weight) on the 54th day. On day 56, blood was collected by puncturing the abdominal aorta artery and the aortic artery was removed. Lipid profile, markers of renal and hepatic injury, lipid peroxidation, and platelet aggregation tests were carried out. The ingestion of extract reduced the lipid peroxidation (aorta and plasma) and platelet aggregation in dyslipidemic rats. The extract did not affect markers of renal and hepatic function as analyzed in this study, suggesting neither impaired liver nor kidney function in these animals. Therefore, our results demonstrate that the extract of leaves of C. arabica L. show antioxidant potential in vitro and in vivo as well as anti-platelet aggregation in dyslipidemic animals.

https://doi.org/10.1590/s2175-97902022e19562

Genetic diversity and aquaculture conservation for a threatened Neotropical catfish
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Farias, Renata S.

; Silva, Bruno C. N. R.

; Nascimento, Wilka V. G.

; Silva, Gênison C.

; Luz, Ronald K.

; Prosdocimi, Francisco

; Figueiredo, Rozzanno A. C. R.

; Carvalho, Daniel C.

; Coimbra, Maria R. M.

Neotropical Ichthyology

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2020, Volume 18 N. 3 elocation e200028

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Devido à importância ecológica de Lophiosilurus alexandri, o presente trabalho avaliou sua representatividade genética, comparando estoques selvagens com plantéis de reprodutores de três larviculturas ao longo do Rio São Francisco. Noventa e sete amostras foram genotipadas com marcadores microssatélites recém-desenvolvidos. Baixos níveis de diversidade genética (número médio de alelos de 4,2) foram detectados em todos os casos, sendo mais severo no cativeiro. Os valores de FST e DEST par a par, as análises do Structure e DAPC mostraram a estruturação dos animais selvagens em dois grupos, e um terceiro formado pelas larviculturas, evidenciando a necessidade de adoção de critérios genéticos para retenção da diversidade genética no cativeiro. Por essa razão, três famílias de irmãos completos foram construídas para selecionar o melhor estimador de parentesco para a espécie e estabelecer os valores mínimos de corte para evitar cruzamentos indesejados. Dois estimadores, Wang (RW) e Lynch & Li (RLL), foram eficientes em refletir as relações de parentesco para irmãos completos nessa espécie. Segundo eles, menos de 50% dos potenciais cruzamentos são recomendáveis nas três larviculturas. A baixa diversidade genética inerente ao L. alexandri destaca a importância de minimizar a consanguinidade e evitar perda de diversidade genética, visando a recuperação da espécie.

Abstract Due to the ecological importance of Lophiosilurus alexandri, the present work evaluated its genetic representativeness by comparing wild stocks to broodstocks that were kept at three restocking hatcheries along the São Francisco River. A total of 97 samples were genotyped for newly developed microsatellite markers. Low levels of genetic diversity (average alleles number of 4.2 alleles) were detected in all cases, being more severe in captive groups. Significant pairwise FST and DEST values, Structure, and DAPC analyses showed that wild animals were structured in two groups, and a third group was formed by captive animals, evidencing the need to adopt genetic criteria to retain genetic diversity in the hatcheries. For this reason, three full-sib families were constructed to select the best relatedness estimator for L. alexandri and establish a cut-off value aimed to avoid full-sibling matings in the hatcheries. Two estimators, Wang (RW) and Lynch & Li (RLL), were accurate in reflecting the relatedness level for full-sibs in this species. According to them, less than 50% of the potential breeding matings in the three hatcheries are advisable. The innate low diversity of L. alexandri highlights the importance of minimizing inbreeding and retaining genetic diversity towards the species recovery.

https://doi.org/10.1590/1982-0224-2020-0028 727 downloads

Conservation of "Paluma" guavas coated with cassava starch and pectin
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Quirino, Anny Karoline Rocha

; Costa, Josenara Daiane de Souza

; Figueiredo Neto, Acácio

; Costa, Marylia de Sousa

; Sánchez-Sáenz, Carolina María

DYNA

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mar 2018, Volume 85 N. 204 Pages 344 - 351

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Este estudio evaluó la conservación post-cosecha de guayabas "Paluma" recubiertas con almidón de yuca y pectina. Las muestras de frutos seleccionadas y desinfectadas, fueron recubiertas con almidón de yuca o pectina a concentraciones de 0 (control), 2, 4 y 6%. Los frutos se almacenaron a 25 ± 1,0 ° C durante 12 días. Cada tres días se realizaron evaluaciones de pérdida de masa, longitud, diámetro, color de piel y pulpa, firmeza de pulpa, sólidos solubles totales, pH y contenido de vitamina C. El experimento fue completamente al azar en un diseño factorial 4 x 5 con cuatro concentraciones de las soluciones y cinco periodos de almacenamiento con tres repeticiones. Los resultados mostraron que los revestimientos a base de almidón de yuca y pectina pueden ser una alternativa para mantener la calidad post-cosecha de las guayabas "Paluma". Las concentraciones de 4 y 6% de ambos tipos de revestimiento proporcionaron buenos resultados con respecto a la retención de algunos atributos. El almidón de yuca mantuvo las frutas más brillantes en comparación con las frutas cubiertas con pectina.

Abstract This study was to evaluate the post-harvest conservation of "Paluma" guavas coated with cassava starch and pectin. The selected and disinfected fruit samples were coated with cassava starch or pectin at concentrations of 0 (control), 2, 4 and 6%. Then, fruits were stored at room temperature (25 ± 1.0°C) for 12 days. Every three days, evaluations of mass loss, length, diameter, color of peel and pulp, pulp firmness, total soluble solids, pH and vitamin C content were held. The experiment was completely randomized in a 4 x 5 factorial design with four concentrations of the solutions and five storage periods with three replications. The results showed that cassava starch and pectin-based coatings can be an alternative for maintaining the post-harvest quality of "Paluma" guavas. Concentrations of 4 and 6% of both types of coating provided good results regarding the retention of some attributes. The cassava starch kept the fruits brighter compared to fruits coated with pectin.

https://doi.org/10.15446/dyna.v85n204.63420 649 downloads

Soil organic matter pools under management systems in Quilombola Territory in Brazilian Cerrado
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Nascimento, Robervone S. de M. P. do

; Ramos, Maria L. G.

; Figueiredo, Cícero C. de

; Silva, Antonio M. M.

; Silva, Stefany B.

; Batistella, Gilmar

Revista Brasileira de Engenharia Agrícola e Ambiental

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apr 2017, Volume 21 N. 4 Pages 254 - 260

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se, neste trabalho, avaliar os compartimentos da matéria orgânica do solo e o índice de manejo de carbono em áreas cultivadas de forma conservacionista e convencional por agricultores quilombolas, em Goiás. As áreas estudadas foram no Cerradão: Cerrado Nativo; Pastagem; Plantio Convencional de Grãos; Plantio Conservacionista de Cultura Perene; e no Cerrado sensu stricto: Cerrado Nativo; Pastagem Natural; Plantio Convencional de Grãos; Plantio Convencional de Cultura Perene. Amostras de solo foram coletadas totalizando cinco repetições em cada área. Foram determinados o carbono orgânico total, as frações das substâncias húmicas, o carbono lábil e o índice de manejo do carbono. A fitofisionomia cerradão apresentou os maiores valores de carbono orgânico total e das frações estáveis da matéria orgânica do solo. As áreas nativas apresentaram baixos teores de carbono lábil. O cultivo conservacionista foi o que apresentou os maiores acúmulos de carbono nos diferentes compartimentos da matéria orgânica e o maior índice de manejo de carbono.

ABSTRACT The aim of this study was to evaluate the stable and labile fractions of soil organic matter and carbon (C) management index in cultivated areas with conservation and conventional management used by Quilombola farmers in the Goiás state, Brazil. The management systems were studied in the areas of Cerradão: Native Cerrado; Pasture; Conventional grain cultivation; Conservation cultivation of perennial crop; and in the sensu stricto Cerrado: Native Cerrado; Natural pasture; Conventional grain cultivation; Conventional cultivation of perennial crop. The study was considered as observational, with five replicates. Total organic C, fractions of humic substances, labile C and C management index were determined. The Cerradão phytophysiognomy had the highest total organic C values and stable soil organic matter fractions. The native areas had low levels of labile C. The conservation cultivation of perennial crop showed the largest accumulation of total organic C in the different fractions of soil organic matter and the highest rates of C management index.

https://doi.org/10.1590/1807-1929/agriambi.v21n4p254-260 903 downloads

Update of the Brazilian floristic list of Algae and Cyanobacteria
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Menezes, Mariângela

; Bicudo, Carlos E. M.

; Moura, Carlos W. N.

; Alves, Aigara M.

; Santos, Alana A.

; Pedrini, Alexandre de G.

; Araújo, Andréa

; Tucci, Andrea

; Fajar, Aurelio

; Malone, Camila

; Kano, Cecília H.

; Sant'Anna, Célia L.

; Branco, Ciro Z.

; Odebrecht, Clarisse

; Peres, Cleto K.

; Neuhaus, Emanuel B.

; Eskinazi-Leça, Enide

; Aquino, Eveline

; Nauer, Fabio

; Santos, Gabriel N.

; Amado Filho, Gilberto M.

; Lyra, Goia M.

; Borges, Gyslaine C.P.

; Costa, Iara O.

; Nogueira, Ina de S.

; Oliveira, Ivania B.

; Paula, Joel. C.de

; Nunes, José M. de C.

; Lima, Jucicleide C.

; Santos, Kleber R.S.

; Ferreira, Leandro C.

; Gestinari, Lísia M.S.

; Cardoso, Luciana S.

; Figueiredo, Marcia A.O.

; Silva, Marcos H.

; Barreto, Maria B.B.B.

; Henriques, Maria C.O.

; Cunha, Maria da G.G.S.

; Bandeira-Pedrosa, Maria E.

; Oliveira-Carvalho, Maria F.

; Széchy, Maria T.M.

; Azevedo, Maria T.P.

; Oliveira, Mariana C. de

; Cabezudo, Mariê M.

; Santiago, Marilene F.

; Bergesh, Marli

; Fujii, Mutuê T.

; Bueno, Norma C.

; Necchi Jr., Orlando

; Jesus, Priscila B.

; Bahia, Ricardo G.

; Khader, Samir

; Alves-da-Silva, Sandra M.

; Guimarães, Silvia M.P.B.

; Pereira, Sonia M.B.

; Caires, Taiara A.

; Meurer, Thamis

; Cassano, Valéria

; Werner, Vera R.

; Gama Jr., Watson A. da

; Silva, Weliton J. da

Rodriguésia

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2015, Volume 66 N. 4 Pages 1047 - 1062

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Apresenta-se uma síntese atualizada de informações sobre algas no Brasil objetivando refinar os dados reunidos até o presente, bem como avaliar os avanços sobre o conhecimento da diversidade de algas no país desde a publicação do Catálogo de Plantas e Fungos do Brasil. Os resultados de 2015 mostraram um acréscimo de 1.250 espécies (35.7%) a um total de 4.747 em relação a 2010. As classes mais diversas em número de espécies foram Bacillariophyceae, Conjugatophyceae, Florideophyceae, Cyanophyceae, Dinophyceae e Euglenophyceae. Bacillariophyceae e Cyanophyceae tiveram o maior acréscimo de espécies no intervalo de cinco anos. A região Sudeste e Sul foram as mais diversas, porém, as regiões Nordeste com os estados do Piauí e Sergipe e Centro-Oeste com os estados de Mato Grosso, Goiás e Distrito Federal destacaram-se no cenário da biodiversidade nacional. Apesar da escassez de taxonomistas e da infraestrutura limitada, os resultados obtidos evidenciaram um avanço significativo no conhecimento da diversidade de algas no país nesse período de cinco anos, iniciando uma mudança quanto as diferenças geográficas regionais.

Abstract An updated synthesis of cyanobacteria and algae information is presented for Brazil aiming to refine the data gathered to date and evaluate the progress of the biodiversity knowledge about these organisms in the country since the publication of the Catálogo de Plantas e Fungos do Brasil. The results of 2015 showed an increase of 1,250 species (35.7%) when compared to 2010, reaching a total of 4,747 species. The most diverse classes in species number were the Bacillariophyceae, Conjugatophyceae, Florideophyceae, Cyanophyceae, Dinophyceae and Euglenophyceae. Bacillariophyceae and Cyanophyceae had the highest increase in species number in the five-year interval. The Southeast and South regions were the most diverse, however, the Northeast, with the states of Piauí and Sergipe, and the Central-west region, with Mato Grosso, Goiás and Distrito Federal, also stood out in the national algal biodiversity scenario. Despite the shortage of taxonomists and limited infrastructure, the results showed a significant improvement in the knowledge regarding the diversity of cyanobacteria and algae in the country during the study period, starting to even out regional geographical differences caused by subsampling.

https://doi.org/10.1590/2175-7860201566408 4865 downloads

Expressed sequence tags in venomous tissue of Scorpaena plumieri (Scorpaeniformes: Scorpaenidae)
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Costa, Fábio L. S.

; Lima, Maria E. De

; Pimenta, Adriano C.

; Figueiredo, Suely G.

; Kalapothakis, Evanguedes

; Salas, Carlos E.

Neotropical Ichthyology

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dec 2014, Volume 12 N. 4 Pages 871 - 878

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Espécies da família Scorpaenidae são responsáveis por acidentes e mortes esporádicas ao longo da costa que habitam. A espécie Scorpaena plumieridesta família povoam a costa Leste e Nordeste do Brasil, causando envenenamento humano caracterizado por sintomas locais e sistêmicos. Em modelos experimentais animais a peçonha induz cardiotoxicidade, efeitos hipotensivos e alterações nas vias aéreas respiratórias. Como primeiro passo para identificar os componentes da peçonha foram isolados os mRNA das glândulas do peixe para produzir uma biblioteca de cDNAs. Esse artigo descreve o sequenciamento parcial de 356 transcritos das glândulas de S. plumieri. Análises em bancos de dados (BLAST) dos transcritos demonstraram que 30% eram sequências desconhecidas, 17% proteínas hipotéticas, 17% relacionadas às enzimas do metabolismo, 14% pertenciam a funções de transdução de sinais e os demais grupos (7-8%) formados por genes relacionados com a expressão de proteínas, proteínas regulatórias e estruturais. Um número considerável destes EST não foi encontrado em bases de dados disponíveis, sugerindo a existência de novas proteínas e/ou funções ainda a serem descobertas. Ao fazer um barrido da biblioteca com anticorpos produzidos contra uma fração das lectinas do veneno de S. plumieri, identificamos vários clones, cuja sequência de DNA mostram semelhanças com lectinas encontradas em peixes. A análise in silicodestes clones confirmam a identidade destas moléculas na glândula de peçonha de S. plumieri.

Species of the family Scorpaenidae are responsible for accidents and sporadic casualties by the shore they inhabit. The species Scorpaena plumierifrom this family populate the Northeastern and Eastern coast of Brazil causing human envenomation characterized by local and systemic symptoms. In experimental animals the venom induces cardiotoxic, hypotensive, and airway respiratory effects. As first step to identify the venom components we isolated gland mRNA to produce a cDNA library from the fish gland. This report describes the partial sequencing of 356 gland transcripts from S. plumieri. BLAST analysis of transcripts showed that 30% were unknown sequences, 17% hypothetical proteins, 17% related to metabolic enzymes, 14% belonged to signal transducing functions and the remaining groups (7-8%) composed by gene related with expressing proteins, regulatory proteins and structural proteins. A considerable number of these EST were not found in available databases suggesting the existence of new proteins and/or functions yet to be discovered. By screening the library with antibodies against a lectin fraction from S. plumierivenom we identified several clones whose DNA sequence showed similarities with lectins found in fish. In silicoanalysis of these clones confirm the identity of these molecules in the venom gland of S. plumieri.

https://doi.org/10.1590/1982-0224-20130149 2423 downloads

Organic matter fractions in soil under coffee with split applications of phosphorus and water regimes
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Araújo, Larissa G.

; Figueiredo, Cícero C. de

; Borges, Inácio B.

; Ramos, Maria L. G.

; Rocha, Omar C.

; Guerra, Antonio F.

Revista Brasileira de Engenharia Agrícola e Ambiental

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oct 2014, Volume 18 N. 10 Pages 1017 - 1022

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A adubação fosfatada e o manejo da irrigação são essenciais para aumentar a produtividade do café; no entanto, há poucas informações sobre o efeito dessas práticas nas frações da matéria orgânica de solos de Cerrado. O objetivo deste trabalho foi avaliar frações da matéria orgânica de um Latossolo cultivado com cafeeiro sob parcelamentos de fósforo (P) e regimes hídricos. O delineamento experimental foi em blocos ao acaso em arranjo fatorial 3 x 2, com três parcelamentos de P (P1: 300 kg ha-1 de P2O5 por ano, sendo 2/3 aplicados em setembro e 1/3 em dezembro; P2: 600 kg ha-1 de P2O5, aplicada no plantio e a cada dois anos e P3: 1.800 kg ha-1 de P2O5 aplicada somente no plantio, necessária para seis anos); dois regimes hídricos (com e sem irrigação) e três repetições. A amostragem de solo foi feita nas camadas de 0-5 e 5-10 cm. Foram determinados o carbono orgânico total (COT) carbono lábil (CL), carbono microbiano (Cmic) e o carbono das frações ácido fúlvico (AF), ácido húmico (AH) e humina (HU). O regime hídrico irrigado do cafeeiro aumentou os teores do COT, CL, Cmic e das frações humificadas da matéria orgânica do solo. De maneira geral, a forma de parcelamento do P exerceu pouca influência sobre as frações da matéria orgânica do solo.

Phosphorus fertilization and irrigation management are essential practices to increase coffee yields, though information is scarce about the effect of these practices on organic matter fractions of soils of the ‘Cerrado’ (savanna-like vegetation). The purpose of this study was to evaluate organic matter fractions of a clayey Oxisol under coffee with split applications of phosphorus (P) and water regimes. The experimental design was a randomized block with 3 x 2 factorial arrangement with three split applications of P (P1: 300 kg ha-1 P2O5 applied annually of which 2/3 applied in September and 1/3 in December; P2: 600 kg ha-1 P2O5 applied at planting and every two years, and P3: 1800 kg ha-1 of P2O5 applied only at planting, corresponding to a 6-year requirement), two water regimes (with and without irrigation) and three replications. Soil from the 0-5 and 5-10 cm layers was sampled. The total organic carbon (TOC), labile carbon (LC), microbial carbon (Cmic), and carbon fractions of fulvic acid (FA), humic acid (HA) and humin (HU) were determined. The irrigation regime of coffee increased the TOC, LC and Cmic levels and the humified fractions of soil organic matter. In general, the form of P splitting had little influence on the fractions of soil organic matter.

https://doi.org/10.1590/1807-1929/agriambi.v18n10p1017-1022 1634 downloads

Growth study and essential oil analysis of Piper aduncum from two sites of Cerrado biome of Minas Gerais State, Brazil
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Oliveira, Gisele L.

; Moreira, Davyson de L.

; Mendes, Aretusa Daniela R.

; Guimarães, Elsie F.

; Figueiredo, Lourdes S.

; Kaplan, Maria Auxiliadora C.

; Martins, Ernane R.

Revista Brasileira de Farmacognosia

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oct 2013, Volume 23 N. 5 Pages 743 - 753

Abstract: En | Text: En | PDF: En

Piper aduncum L., Piperaceae, stands out due to its biological activities, however, it is still found in the wild and little is known about its agronomic point of view. The aim of this study was to evaluate the growth and to analyze the chemical composition of essential oils from leaves of P. aduncum collected in two different sites of Cerrado as well as in cultivated plants. The cultivation was installed out in a greenhouse using cuttings of adult specimens. Essential oils were obtained from fresh leaves. Plants from the two studied locations showed erect growth habit and behavior of linear growth. The essential oils composition of P. aduncum from Bocaiuva did not differ between wild and cultivated plants, as the major substance identified as 1,8-cineole. The plants from Montes Claros site showed a distinct concentration for the two samples, being the major substance characterized as transocimene (13.4%) for wild and 1,8-cineole (31.3%) for cultivated plants. Samples from both locations showed a similar essential oil composition in cultivars. Our results showed that P. aduncum cultivation is feasible and the variation in chemical composition of the two sites may indicate an environmental influence, since chemical and isoenzyme analysis did not show great differences.

https://doi.org/10.1590/S0102-695X2013000500005 3098 downloads

10.

Chemical study and larvicidal activity against Aedes aegypti of essential oil of Piper aduncum L. (Piperaceae)
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OLIVEIRA, GISELE L.

; CARDOSO, SHEILA K.

; LARA JÚNIOR, CéLIO R.

; VIEIRA, THALLYTA M.

; GUIMARãES, ELSIE F.

; FIGUEIREDO, LOURDES S.

; MARTINS, ERNANE R.

; MOREIRA, DAVYSON L.

; KAPLAN, MARIA AUXILIADORA C.

Anais da Academia Brasileira de Ciências

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2013, Volume 85 N. 4 Pages 1227 - 1234

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMOPiper aduncum L. é utilizada na medicina popular para o tratamento de doenças respiratórias e inflamatórias. O objetivo desse estudo foi analisar o óleo essencial das folhas de P. aduncum coletadas no Cerrado brasileiro, Norte de Minas Gerais, assim como avaliar a atividade larvicida desse óleo e de sua substância majoritária. O óleo essencial foi analisado por cromatografia em fase gasosa acoplada à espectrometria de massas e por cromatografia em fase gasosa acoplada a detector por ionização de chamas, que permitiu a caracterização de 23 substâncias (monoterpenos: 90,4%; sesquiterpenos: 7,0%). O componente majoritário foi o 1,8-cineol (53,9%). O óleo essencial desse exemplar mostrou ser muito diferente do óleo da mesma espécie. Larvas de Aedes aegypti foram expostas a diferentes concentrações do óleo essencial e do 1,8-cineol. Após 24h do tratamento com o óleo registrou-se uma taxa de mortalidade de 100% das larvas nas concentrações de 500 e 1,000 ppm. Após 48h a taxa de mortalidade foi de 80% e 50% para as concentrações de 250 e 100 ppm, respectivamente. A CL50 obtida após 24h foi estimada em 289,9 ppm e após 48h foi de 134,1 ppm. A substância majoritária 1,8-cineol não apresentou atividade larvicida.

ABSTRACTPiper aduncum L. is used in folk medicine to treat respiratory and inflammatory diseases. The aim of this study was to analyze the essential oil from leaves ofP. aduncum collected in the Brazilian Cerrado, North of Minas Gerais, as well as to evaluate the larvicidal activity of this oil and of its major constituent. The essential oil was analyzed by gas chromatography coupled to flame ionization detector and gas chromatography coupled to mass spectrometry that allowed characterizing 23 compounds (monoterpenes: 90.4%; sesquiterpenes: 7.0%). The major component was 1,8-cineole (53.9%). This oil showed to be very different from those obtained from the same species. Larvae of A. aegypti were exposed to different concentrations of the essential oil and 1,8-cineole. The mortality rate of 100% was obtained after 24h of treatment with the oil at concentrations of 500 and 1,000 ppm. After 48h of treatment, the mortality rate was 80% and 50% for concentrations of 250 and 100 ppm, respectively. The LC50 obtained after 24h was estimated in 289.9 ppm and after 48h was 134.1 ppm. The major compound 1,8-cineole showed no larvicidal activity.

https://doi.org/10.1590/0001-3765201391011 18969 downloads

11.

Matéria orgânica e infiltração da água em solo sob consórcio milho e forrageiras
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Sato, Juliana H

; Figueiredo, Cícero C. de

; Leão, Tairone P

; Ramos, Maria L. G

; Kato, Eiyti

Revista Brasileira de Engenharia Agrícola e Ambiental

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feb 2012, Volume 16 N. 2 Pages 189 - 193

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A infiltração é o processo pelo qual a água penetra no solo através de sua superfície e sua dinâmica pode ser condicionada por diversos fatores, dentre eles os sistemas de manejo do solo adotados. O objetivo deste trabalho foi estimar a velocidade de infiltração da água e os teores de matéria orgânica do solo em sistema de consórcio milho e forrageiras. O experimento foi realizado em delineamento de blocos casualizados, com três repetições. Foram estudados os efeitos de cinco tratamentos submetidos ao manejo de plantio direto: 1) Milho Solteiro (Zea mays L. ); 2) Braquiária Humidícola (Brachiaria humidicola); 3) Consórcio Milho e Braquiária Humidícola; 4) Capim Aruana (Panicum maximum cv. Aruana); 5) Consórcio Milho e Capim Aruana. Utilizou-se, para determinação da velocidade de infiltração básica (VIB), o método dos infiltrômetros de cilindros concêntricos. Outros atributos físicos do solo e a matéria orgânica do solo foram avaliados buscando-se associá-los aos valores de VIB encontrados. Os resultados revelaram que sob plantio direto os sistemas apresentaram elevados valores de VIB. O sistema Milho Solteiro apresentou a maior VIB e a inserção de forrageiras nos sistemas provocou redução da VIB; todos os sistemas apresentaram diminuição dos teores de matéria orgânica em profundidade.

Infiltration is a process by which water penetrates through the soil by its surface, and its dynamics can be influenced by several factors, among them, the soil management system adopted. The objective of this work was to estimate water infiltration rate and soil organic matter content in maize and forage intercropping systems. The experiment was conducted in a randomized block design with three replications. Five treatments under no-tillage management were evaluated: 1) Corn (Zea mays L. ), 2) Brachiaria humidicola (Brachiaria humidicola), 3) Corn and Brachiaria humidicola intercropping, 4) Aruana grass (Panicum maximum cv. Aruana), 5) Corn and Aruana grass intercropping. The double ring infiltrometer method was used to determine the basic infiltration rate (VIB). Soil physical properties and soil organic matter were evaluated and correlated with VIB values. Results revealed high VIB values for no-tillage. The highest VIB values were observed in the corn treatment. VIB values decreased with the inclusion of forages. All cultivation systems showed a decrease of organic matter with depth.

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12.

Chemical composition fluctuations in roots of Plumbago scandens L. in relation to floral development
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Paiva, Selma R.

; Lima, Lucilene A.

; Figueiredo, Maria Raquel

; Kaplan, Maria Auxiliadora C.

Anais da Academia Brasileira de Ciências

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dec 2011, Volume 83 N. 4 Pages 1165 - 1170

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Plumbago scandens L. é uma espécie brasileira tropical/subtropical que ocorre ao longo da costa. Quimicamente, é principalmente representada por naftoquinonas, flavonóides, terpenóides e esteróides. objetivo do presente trabalho é estudar mudanças quantitativas da produção metabólica nas raízes de Plumbago scandens durante diferentes estágios de desenvolvimento fisiológico, relativos à floração. Os resultados indicaram a presença de quatro substâncias nos extratos: plumbagina, epi-isoshinanolona, ácido palmítico e sitosterol, independente do estágio de desenvolvimento. A naftoquinona plumbagina tem sempre mostrado ser o componente majoritário de todos os extratos. Naftoquinonas exibiram seus maiores conteúdos durante a floração, enquanto o conteúdo dos dois outros componentes decresceu durante este estágio, revelando um perfil inverso. A composição química modificou dependendo das necessidades da planta.

Plumbago scandens L. is a Brazilian tropical/subtropical species that occurs along the coast. Chemically it is mainly represented by naphthoquinones, flavonoids, terpenoids and steroids. The aim of the present work is to study quantitative changes in the root metabolic production of Plumbago scandens during different physiologic developmental stages relative to floration. The results indicated the presence of four substances in the extracts: plumbagin, epi-isoshinanolone, palmitic acid and sitosterol, independent on developmental stage. The naphthoquinone plumbagin has always showed to be the major component of all extracts. Naphthoquinones exhibited their highest content during floration, while the content of the two others components decreased during this stage, revealing an inverse profile. The chemical composition changed depending on the plant requirements.

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13.

Synthesis and antiproliferative activity of novel limonene derivatives with a substituted thiourea moiety
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Figueiredo, Isis M.

; Santos, Luciane V. dos

; Costa, Willian F. da

; Carvalho, João E. de

; Silva, Cleuza C. da

; Sacoman, Juliana L.

; Kohn, Luciana K.

; Sarragiotto, Maria H.

Journal of the Brazilian Chemical Society

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oct 2006, Volume 17 N. 5 Pages 954 - 960

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

No presente trabalho descrevemos a síntese e a avaliação da atividade antiproliferativa, frente a linhagens de células tumorais humanas, de derivados do R-(+)-limoneno (3-18) contendo uma unidade tiouréia substituída. Os derivados com substituintes arílicos (3-6) exibiram atividade citostática frente a todas linhagens testadas, com inibição de 50% do crescimento celular (GI50) em concentrações na faixa de 2,5 a 24 µmol L-1. Os compostos 3, 10, 12 e 16 foram os mais ativos, com GI50 na faixa de 0,41 a 3,0 mmol L-1, frente a diferentes linhagens celulares.

A series of R-(+)-limonene derivatives bearing a substituted thiourea moiety (3-13) and five S-methyl analogs (14-18) were synthesized and evaluated for their in vitro antiproliferative activity against human cancer cell lines. Compounds bearing aromatic substituents (3-6) exhibit cytotastic activity in the full panel of cell lines tested, with GI50 values in the range of 2.5 to 24 µmol L-1. Compounds 3, 10, 12 and 16 were the most active with GI50 values in the range of 0.41 to 3.0 µmol L-1, against different cell lines.

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14.

CLL: chromosomal abnormalities (FISH) and their relation with clinical stage, CD38 and ZAP-70
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Nascimento, Marilia C.

; Yamamoto, Mioko

; Rodrigues, Maria Madalena

; Franco, Luciana F.

; Kimura, Elisa Y. S.

; Vasconcelos, Yuri

; Oliveira, José S. R.

; Figueiredo, Vera L. P.

; Chauffaille, Maria de Lourdes L. F.

Revista Brasileira de Hematologia e Hemoterapia

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mar 2006, Volume 28 N. 1 Pages 5 - 10

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A leucemia linfocítica crônica (LLC) é o tipo de leucemia mais prevalente no Ocidente e é caracterizada por curso clínico extremamente variável. O objetivo deste estudo foi detectar as anomalias cromossômicas mais freqüentes em pacientes com LLC, empregando a técnica FISH, e correlacioná-las com idade, sexo, estádio clínico, expressão de CD 38 e ZAP-70. Foram encontradas alterações cromossômicas em 51,7% dos pacientes. A mais freqüente foi a del 13q14, observada em 34,5% dos casos e que esteve associada a outras anomalias em 17,2%. Deleção 17p13 foi encontrada em 17,2% e trissomia 12 em 13,8% (isolada em 6,9% e associada à del 13q14 em 6,9%). Deleção 11q22 foi observada em um caso em concomitância à del 13q14. Para melhor avaliar a relação entre alteração cromossômica e outros fatores prognósticos em LLC, dois grupos citogenéticos foram considerados: favorável (deleção 13q isolada e ausência de alterações) e desfavorável (trissomia 12, deleção 17p13, deleção 11q22 e duas anomalias simultâneas). As alterações desfavoráveis foram mais freqüentemente observadas em indivíduos jovens (<60 anos) e em mulheres (70%)(p=0,04). Em relação ao sistema de estadiamento de Binet, houve tendência dos pacientes com alterações cromossômicas desfavoráveis apresenteram-se nos estágios B e C enquanto no grupo favorável prevaleceram aqueles com estágio A. Em adição, pacientes com achados citogenéticos de prognóstico desfavorável tiveram tendência a expressar proteínas CD 38 e ZAP-70.

Chronic lymphocytic leukemia is the most prevalent type of leukemia in the West. It is characterized by an extremely variable clinical course. The aim of the study was to detect the most frequent chromosomal abnormalities in patients with CLL using FISH, and assess them regarding age, gender, clinical stage and CD38 and ZAP-70 expressions. We found 51.7% of the patients with chromosome abnormalities. The most frequent one was del 13q14 in 34.5% of cases. It was associated to other alterations in 17.2%. 17p13 deletions were found in 17.2% and trisomy 12 in 13.8% (in isolation in 6.9% and associated to del 13q14, in 6.9% of the cases). An 11q22 deletion was found in one case associated to a 13q14 deletion. To better evaluate the relationship between chromosome aberrations and other prognostic factors in CLL, two cytogenetics groups were considered: favorable (13q deletion in isolation and no alteration) and unfavorable outcomes (trisomy 12, 17p13 deletion, 11q22 deletion and two simultaneous alterations).The unfavorable alterations were more frequently seen among young individuals (<60y). There were more females (70%) than males in this group (p=0.04). In relation to the Binet's staging system, patients with unfavorable cytogenetic alterations, tended to be B and C stages, while in the favorable group prevailed patients in stage A. Additionally, patients with poor prognostic cytogenetics tended to express CD38 and ZAP-70 proteins.

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15.

Maxadilan (MAX) - Proteína salivar de Lutzomyia longipalpis: detecção de anticorpos antiMAX em leishmaniose tegumentar americana (LTA) e expressão gênica e protéica de MAX em Lutzomyia neivai
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Aires, Juliana Machado

; Chociay, Maria Fernanda

; Nascimento, Margarida M. P.

; Figueiredo, José Fernando de C.

; Roselino, Ana Maria F.

Anais Brasileiros de Dermatologia

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dec 2005, Volume 80 Suppl. 3 Pages S333 - S338

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

FUNDAMENTOS: AA proteína MAX, componente salivar de Lutzomyia longipalpis, vetor do calazar ou leishmaniose sistêmica, tem sido empregada como vacina para leishmaniose tegumentar experimental, com funções de vasodilatação e imunomodulação. OBJETIVOS: Detectar anticorpos séricos antiMAX em pacientes com LTA e verificar a expressão de MAX em L. neivai, vetor da LTA na região em estudo. MÉTODOS: Anticorpos antiMAX foram detectados por Elisa em soro de 42 pacientes com LTA e 63 controles. A extração de proteínas e de DNA de L. longipalpis (controle positivo) e de L. neivai foi realizada pelo método Trizol e seguida pela detecção de proteínas por eletroforese e pela expressão gênica de MAX por PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) com as enzimas Hha I e Rsa I. RESULTADOS: Títulos maiores de anticorpos antiMAX foram observados na LTA (p=0,0132). A eletroforese de proteínas mostrou frações semelhantes para L. longipalpis e L. neivai, tendo-se observado para ambos fração protéica de peso molecular similar à proteína MAX. A expressão gênica de MAX em L. longipalpis e L. neivai foi confirmada por PCR-RFLP. CONCLUSÕES: A presença de antiMAX nos grupos em estudo tornou imprescindível a pesquisa de MAX no vetor de LTA da região, tendo sido registrada pela primeira vez expressão protéica e gênica de MAX em L. neivai. Detecção de antiMAX em controles confirma exposição a picadas de flebótomos. Títulos de anticorpos antiMAX maiores na LTA sugerem exposição prévia e natural à picada e, conseqüentemente, à proteína MAX, não protegendo da doença e desfavorecendo seu emprego em vacinação.

BACKGROUND: The protein MAX, salivary component of Lutzomyia longipalpis, vector of calazar or systemic leishmaniasis, has been used as vaccine for experimental tegumentar leishmaniasis, which vasodilatory and immunomodulatory functions are described. OBJECTIVES: Our purpose was to detect antibodies antiMAX in sera samples from patients with ATL and to verify the genetic and protein expression of MAX in L. neivai, phlebotomy vector of ATL in the area of study. METHODS: Antibodies antiMAX were detected by ELISA in sera from 42 patients with ATL and 63 controls. The extraction of proteins and of DNA from L. longipalpis (positive control) and L. neivai was accomplished by the method Trizol, following for the detection of proteins by electrophoresis, and genetic expression of MAX by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) with the enzymes Hha I and Rsa I. RESULTS: Increased titles of antibodies antiMAX were observed in ATL, compared to controls (p=0,0132). Electrophoreses of proteins showed similar fractions for L. longipalpis and L. neivai, and for both a protein fraction with molecular weight similarity to MAX was observed. The genetic expression of MAX in L. longipalpis and L. neivai was confirmed by PCR-RFLP. CONCLUSIONS: The description of antibodies antiMAX in ATL patients and controls turned indispensable the research of MAX in the phlebotomy vector of ATL in the area of study. For the first time, it was registered protein and genetic expression of MAX in L. neivai. The antiMAX detection in controls confirms the previous exposition to prick of phlebotomies. Increased titles of antibodies antiMAX in ATL patients suggest previous and natural exposition to the bite and, consequently, to the protein MAX, not protecting them of disease and discouraging its employment in vaccination.

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