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Impact of the lactate dehydrogenase in association with the International Staging System prognostic score in multiple myeloma patients treated in real life
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Lopes, Maricy Almeida Viol Ferreira

; Higashi, Fabiana

; Crusoe, Edvan de Queiroz

; Peres, Ana Lucia Miguel

; Cury, Priscilla

; Hungria, Vania Tietsche de Moraes

Hematology, Transfusion and Cell Therapy

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2023, Volume 45 N. 2 Pages 259 - 265

Abstract: En | Text: En | PDF: En

Abstract Introduction Multiple myeloma is characterized by proliferation of clonal plasma cells. The identification of prognostics factors to identify patient's risk is important. Among the studied factors, it was identified of relevant importance the lactic dehydrogenase. Objectives To evaluate the impact of the value of DHL in combination with the score ISS in the medium patients overall survival (OS). Methods It is a retrospective cohort with 252 patients with MM recently-diagnosed that attendance in the institution of the study. Results To evaluate the association between DHL and ISS, we found 6 new groups to be analyzed: ISS I and normal DHL with medium overall survival not reached, and with DHL loud with medium OS of 69,8 months, ISS II and normal DHL with medium overall survival of 78,8 months and with DHL loud with medium OS of 73,9 months, ISS III and normal DHL with medium overall survival of 46,7 months and with DHL loud with medium OS of 45,5 months. Conclusion Through the association of ISS I and normal DHL, ISS III and high DHL and others combinations, we build a new score with superior impact prognostic in our population treated in real life.

https://doi.org/10.1016/j.htct.2022.07.005

Superiority of the triple combination of bortezomib, cyclophosphamide and dexamethasone versus cyclophosphamide, thalidomide and dexamethasone in patients with newly diagnosed multiple myeloma, eligible for transplantation
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Crusoe, Edvan De Queiroz

; Higashi, Fabiana

; Martinez, Gracia

; Bittencourt, Rosane

; Pinto Neto, Jorge Vaz

; Sousa, Lais

; Santucci, Rodrigo

; Magalhães, Roberto José Pessoa

; Colli, Gilberto

; Nunes, Renata Ferreira Marques

; Ribeiro, Glaciano

; Nicacio, Jandir

; Zanella, Karla Richter

; Kutner, Jose Mauro

; Magalhaes, Andre

; Leao, Danielle

; Hallack Neto, Abrahão Elias

; Braga, Walter

; Souza, Emanuella G

; Guimaraes, Antonio Julio A.M.

; Durigon, Giovanna Steffenello

; Laks, Dani

; Maiolino, Angelo

; Hungria, Vania Tietsche de Moraes

Hematology, Transfusion and Cell Therapy

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jun 2020, Volume 42 N. 2 Pages 118 - 124

Abstract: En | Text: En | PDF: En

ABSTRACT Background: The treatment of multiple myeloma (MM) has evolved significantly in the past decade, and new drug combinations have improved the response rates and prolonged survival. Studies comparing different induction chemotherapy regimens have shown that triple combinations have better results than double combinations. However, comparisons among different triple combinations are rare in the literature. Methods: We retrospectively compared two triple combinations comprising bortezomib, cyclophosphamide and dexamethasone (VCD) versus thalidomide, cyclophosphamide and dexamethasone (CTD), and aimed at identifying which of the two combinations would yield better response rates following four induction cycles prior to hematopoietic cell transplantation in patients with untreated multiple myeloma. Results: We retrospectively reviewed the medical records of 311 patients from 24 different centers.The VCD regimen was used as induction therapy by 117 (37.6%) patients, whereas 194 (62.4%) patients received the CTD regimen. After four cycles of induction on an intention-to-treat basis, 54% of the patients in the VCD group achieved at least very good partial response versus 42.8% in the CTD group (p = 0.05). We observed no difference in neuropathy or thrombotic events rates among the two regimens. Conclusion: Our results corroborate the superiority of the triple combination regimes containing bortezomib over the triple combination with thalidomide as pre ASCT induction therapy in MM.

https://doi.org/10.1016/j.htct.2019.05.004 283 downloads

Local effect of simvastatin combined with different osteoconductive biomaterials and collagen sponge on new bone formation in critical defects in rat calvaria
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Sousa, Dircilei Nascimento de

; Roriz, Virgílio Moreira

; Oliveira, Guilherme José Pimentel Lopes de

; Duarte, Wagner Rodrigues

; Pinto, Leonardo Nogueira de Miranda Pereira

; Vianna, Leonora Maciel de Souza

; Carneiro, Fabiana Pirani

; Ferreira, Vania Maria Moraes

Acta Cirúrgica Brasileira

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2020, Volume 35 N. 1 elocation e202000102

Abstract: En | Text: En | PDF: En

Abstract Purpose To evaluate the local effect of simvastatin (SVT) combined with deproteinized bovine bone (DBB) with hydroxyapatite/β-tricalcium phosphate biphasic ceramics (HA/TCP) and with collagen sponge (CS) on bone repair in critical size defects (CSDs) in rat calvaria. Methods Forty-two 5-mm diameter CSDs were made bilaterally in the calvaria of 18 rats. The animals were allocated according to the type of biomaterial and associations used to fill the CSD. After 8 weeks, the animals were euthanized, and their calvaria were evaluated for repaired tissue composition using histologic and histometric analyses. Results In the histometric analysis, the use of SVT showed to increase bone formation in the CSDs when combined with all the bone substitutes tested in this study (p<0.05). Greater bone formation was observed in the groups with SVT compared to the groups without SVT. Conclusions The use of SVT without the need for a vehicle and combined with a commercially available biomaterial may be a cheaper way to potentiate the formation of bone tissue without the need to produce new biomaterials. Therefore, SVT combined with DBB induced significantly greater new bone formation than did the other treatments.

https://doi.org/10.1590/s0102-865020200010000002 819 downloads

A utilização do Método de Rorschach no diagnóstico diferencial da Esquizofrenia e Transtorno Dissociativo de Identidade
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Faria, Marcello de Abreu

; Prado, Euthymia Brandão de Almeida

; Souza, Wânia Cristina de

; Martins, Vaneila Ferreira

; Ferreira, Vania Moraes

Psicologia: Teoria e Pesquisa

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2019, Volume 35 elocation e3521

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo deste artigo foi investigar a prevalência do Transtorno Dissociativo de Identidade (TDI) em dez pacientes diagnosticados com esquizofrenia com característica paranóide, com base no Método de Rorschach. Foram registrados indicadores ou sintomas psicológicos que caracterizaram o diagnóstico diferencial desses pacientes, analisadas diferenças clínicas específicas e verificado o enquadramento dos dados obtidos, com a aplicação da Técnica de Rorschach. Para fins de diagnóstico diferencial, observou-se que dois pacientes apresentaram sintomas diferenciais aos da esquizofrenia, sendo que um deles apresentou sintomas dissociativos, não sendo confirmada a prevalência do TDI entre os esquizofrênicos. No caso da esquizofrenia e do TDI, devido às características similares que apresentam, avaliações multidisciplinares são indicadas para se fazer o diagnóstico diferencial entre eles.

ABSTRACT The aim of this article was to investigate the prevalence of the Dissociative Identity Disorder (DID) in ten patients diagnosed with schizophrenia with paranoid characteristic, based on the Rorschach approach. Psychological symptoms that characterize the differential diagnosis of these patients were registered, specific clinical differences were analyzed and the framework of the data obtained with the application of the Rorschach approach. For purposes of differential diagnosis, it was observed that two patients had differential symptoms to schizophrenia, one of which showed dissociative symptoms, not confirmed the prevalence of DID among schizophrenics. In the case of schizophrenia and DID due to exhibit similar characteristics, multidisciplinary evaluations are set to make a differential diagnosis between them.

https://doi.org/10.1590/0102.3772e3521 7294 downloads

The effect of simvastatin on relapse of tooth movement and bone mineral density in rats measured by a new method using microtomography
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Vieira, Giovanni Modesto

; Chaves, Sacha Braun

; Ferreira, Vânia Maria Moraes

; Freitas, Karina Maria Salvatore de

; Amorim, Rivadávio Fernandes Batista

Acta Cirúrgica Brasileira

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may 2015, Volume 30 N. 5 Pages 319 - 327

Abstract: En | Text: En | PDF: En

PURPOSE: To evaluate the effect of simvastatin on relapse of tooth movement in rats using microtomography (micro CT), as well as the correlation of bone density with the orthodontic relapse.METHODS: Twenty-five adult male Wistar rats, divided into two groups, had stainless steel springs installed on left maxillary first molar. The molars were moved for 18 days, and after removing the springs, were applied by oral gavage, 5mg/kg of simvastatin in the experimental group for 20 days. Tooth relapse was assessed with a micro CT scanner, and the images chosen through the Data Viewer software 1.5.0.0 had their measurement guides made and checked by the software Image ProR plus 5.1, and compared by Mann-Whitney test. After rats were sacrificed, bone mineral density was evaluated by micro CT through the software CT Analyzer 1.13 and compared by independent T-test, as well as by Spearman correlation test.RESULTS: Relapse and bone mineral density (BMD) was lower in the experimental group than in the control group, however without a statistically significant difference.CONCLUSION: Simvastatin did not inhibit the relapse of tooth movement in rats, and there was no correlation between bone density and orthodontic relapse.

https://doi.org/10.1590/S0102-865020150050000003 2816 downloads

Evaluation of antinociceptive and anti-inflammatory activities of the topical preparation of Cipura paludosa (Iridaceae)
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Silva Neto, José Antônio Paixão da

; Menezes, Larissa Dias

; Gomes, Gúbio Oliveira

; Cunha, Elise Marques Freire

; Azevedo, Mariângela Soares de

; Ferreira, Vania Moraes

; Silva, Mônica Valero da

Acta Amazonica

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jun 2014, Volume 44 N. 2 Pages 263 - 270

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Allho do mato (Cipura paludosa, Iridaceae) é uma planta medicinal encontrada na floresta Amazônica, norte do Brasil. Essa planta tem sido usada para tratar dores, processos inflamatórios e infecciosos. O objetivo do estudo foi avaliar a ação anti-inflamatória e antinociceptiva do extrato bruto etanólico de Cipura paludosa, nas concentrações que variaram entre 2,0 e 4,0%, em formulações de cremes óleo e água de uso tópico. A estabilidade físico-química das formulações foi monitorada ao longo de um período de seis meses, com a aplicação de ensaios de estabilidade acelerada. A fim de avaliar as atividades anti-inflamatória e antinociceptiva, utilizou-se um teste de edema de pata induzido por carragenina e um teste de formalina, respectivamente. O teste de edema de pata mostrou que houve uma diferença significativa no grupo controle em relação aos tratamentos. O teste da formalina não confirmou efeito antinociceptivo dos tratamentos com o extrato, na primeira fase do ensaio. No entanto, a diferença estatística foi confirmada para os tratamentos em relação ao controle na segunda fase. As atividades antinociceptiva e anti-inflamatória das preparações com Cipura paludosa confirmam de forma parcial o uso etno-médico desta planta.

Alho do mato (Cipura paludosa, Iridaceae) is a medicinal plant found in the Amazon rain forest, North of Brazil. It has been used to treat algic, inflammatory and infectious processes. The aim of this study was to evaluate the anti-inflammatory and antinociceptive action of the crude Cipura paludosa ethanolic extract at concentrations ranging between 2.0 and 4.0% in Oil and Water cream formulations for topical use. The physical-chemical stability of the formulations was monitored over a six-month period with the use of accelerated stability tests. In order to evaluate the anti-inflammatory and antinociceptive activities, we used a paw edema test induced by carrageenan and a formalin test, respectively. The paw edema test showed that there was a statistical difference in the control group in relation to the treatments. The formalin test did not confirm antinociceptive action of the treatments with the extract in the early phase of the test. However, statistical difference was confirmed for the treatments in relation to the control in the late phase. The antinociceptive and anti-inflammatory activities of Cipura paludosa preparations, as demonstrated in the results, at least partially support the ethno-medical uses of this plant.

https://doi.org/10.1590/S0044-59672014000200012 3925 downloads

Behavioural and cognitive effects of simvastatin dose used in stimulation of bone regeneration in rats
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Sousa, Dircilei Nascimento de

; Santana, Washington Macedo de

; Ferreira, Vania Moraes

; Duarte, Wagner Rodrigues

Acta Cirúrgica Brasileira

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mar 2014, Volume 29 N. 3 Pages 151 - 157

Abstract: En | Text: En | PDF: En

PURPOSE: To analyze the effects of simvastatin (SVT) in the locomotion, anxiety and memory of rats, as a reflection of the administration of a minimum dose capable of stimulating bone regeneration in defects in the calvariae.METHODS: Surgical procedures were performed in 15 female Wistar rats, 2-month old, to insert the grafting material regenerator (Bone-ceramic(r)) and/or SVT, followed by behavioural and cognitive assessments in the 7th, 30th and 60th days post surgery.RESULTS: The SVT locally applied with the goal of bone regeneration in defects created in rat calvariae does not interfere with locomotion, anxiety levels and/or memories of rats, except for the first week following surgery, when an anxiolytic effect was observed, as a result of a possible central action.CONCLUSION: Failure to provoke any response within 30 and 60 days post surgical procedures suggests that SVT may constitute a good choice in stimulating bone regeneration without affecting the long term neural functions.

https://doi.org/10.1590/S0102-86502014000300002 3511 downloads

Psychophysiological consequences arising from the stress of everyday life and work activities
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Ferreira Martins, Vaneila

; Moraes Ferreira, Vania

; Guilhem, Dirce

Salud mental

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apr 2013, Volume 36 N. 2 Pages 141 - 147

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT Introduction Stress is a common phenomenon among nursing professionals and has received special attention, particularly in relation to the lifestyle characteristic of the contemporary world, which promotes various diseases due to an organic imbalance. Objective and method The purpose of this study was to evaluate the psychophysiological consequences of activities in the workplace and everyday life in 408 nursing professionals and to identify ways to improve personal and occupational well-being. A quantitative-qualitative, descriptive and exploratory population-based study was performed. Results The results showed that the majority of the sample worked double shifts and viewed themselves as "sometimes stressed". A significant proportion of the professionals experienced verified illnesses, including many chronic-degenerative diseases and neuropathies. Pharmacotherapy was the main treatment used to alleviate the psychophysiological problems and most professionals never used alternative therapies (e.g., psychotherapy) as a way to reduce their stress. Conclusions The results suggest that the professionals studied demonstrated psychophysiological alterations resulting from stressors that arise in the workplace and in everyday life. The response to these stressors, although it was frequently not perceived, culminates in several chronic diseases. To cope with these stressful events, individuals must increase their self-esteem and cultivate a more harmonious and balanced work and personal life.

RESUMEN Introducción El estrés es un fenómeno común entre los profesionales de enfermería. Ha recibido especial atención, especialmente en lo que se relaciona con las características del estilo de vida del mundo contemporáneo, que pueden generar distintas enfermedades resultantes del desequilibrio orgánico. Objetivo y método El objetivo de este estudio fue evaluar las consecuencias psicofisiológicas de las actividades realizadas en el lugar de trabajo y en la vida cotidiana. Fueron entrevistados 408 profesionales de enfermería para identificar posibilidades de promover el bienestar personal y ocupacional. Fue realizado un estudio cualitativo-cuantitativo, descriptivo y exploratorio de base poblacional. Resultados Los resultados demostraron que la mayoría de los entrevistados tenían doble jornada de trabajo y se consideraban "algunas veces estresados." Una proporción significativa de los profesionales desarrolló enfermedades, incluidas algunas enfermedades crónico-degenerativas y neuropatías. El tratamiento medicamentoso fue la principal alternativa para aliviar los problemas psicofisiológicos. La mayoría de los profesionales jamás hicieron uso de terapias alternativas (por ej., la psicoterapia) como estrategia para reducir su tensión. Conclusiones Los resultados sugieren que los profesionales entrevistados desarrollarán alteraciones psicofisiológicas resultantes del estrés proveniente del lugar de trabajo y de la vida cotidiana. Para hacer frente a estos acontecimientos agotadores, los profesionales deben aumentar su amor propio y cultivar un trabajo más armonioso y una vida personal más equilibrada.

4206 downloads

Efeitos da bromoprida na cicatrização de anastomoses no cólon esquerdo de ratos
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Silva, Silvana Marques e

; Ferreira, Vânia Maria Moraes

; Carneiro, Fabiana Pirani

; Feres, Omar

; Oliveira, Paulo Gonçalves de

; Sousa, João Batista de

Revista do Colégio Brasileiro de Cirurgiões

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dec 2011, Volume 38 N. 6 Pages 429 - 434

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Avaliar os efeitos da bromoprida sobre a formação de aderências e a cicatrização de anastomoses de cólon esquerdo de ratos. MÉTODOS: Foram incluídos 40 ratos, divididos em dois grupos contendo 20 animais, para administração de bromoprida (grupo de estudo- E) ou solução fisiológica (grupo controle- C). Cada grupo foi dividido em subgrupos contendo 10 animais cada, para eutanásia no terceiro (E3 e C3) ou no sétimo dia (E7 e C7) de pós-operatório. Os ratos foram submetidos à secção do cólon esquerdo e anastomose término-terminal. No dia da relaparotomia, foi avaliada a quantidade total de aderências e removido um segmento colônico contendo a anastomose para análise histopatológica, da força de ruptura e da concentração de hidroxiprolina. RESULTADOS: Não houve diferença entre os grupos em relação à evolução clínica. Dois animais do grupo de estudo apresentaram deiscência de anastomose bloqueada. Os animais que receberam bromoprida apresentaram número de aderências intracavitárias e aderências à anastomose semelhantes ao grupo controle. As anastomoses dos animais do grupo E3 apresentaram menor resistência de ruptura do que as do grupo C3 (p=0,04). Este efeito não ocorreu no sétimo dia de pós-operatório (p=0,37). Não houve diferença significativa entre os grupos em relação à histopatologia ou concentração de hidroxiprolina das anastomoses. CONCLUSÃO: O uso da bromoprida está associado à diminuição da resistência tênsil de anastomoses do cólon esquerdo de ratos no terceiro dia de pós-operatório.

OBJECTIVE: To evaluate the effects of bromopride on the formation of adhesions and anastomotic healing in the left colon of rats. METHODS: We divided 40 rats into two groups of 20 animals, administration of bromopride (study group-E) or saline (control group-C). Each group was divided into subgroups containing 10 animals each for euthanasia in the third (C3 and E3) or the seventh (E7 and C7) postoperative days. The rats were submitted to section of the left colon and end-to-end anastomosis. On the day of reoperation, we evaluated the total amount of adhesions and removed a colonic segment containing the anastomosis for histopathological analysis, assessment of rupture strength and hydroxyproline concentration. RESULTS: There was no difference between groups in relation to clinical outcome. Two animals in the study group had blocked anastomotic leakage. The animals that received bromopride had the number of intracavitary adhesions and adhesions to the anastomosis similar to the control group. The anastomoses from the group E3 animals showed lower resistance to rupture the one from the C3 group (p = 0.04). This effect did not occur on the seventh postoperative day (p = 0.37). There was no significant difference between groups in relation to histopathology and hydroxyproline concentration in the anastomoses. CONCLUSION: The use of bromopride was associated with decreased tensile strength of left colon anastomosis in rats in the third postoperative day.

2980 downloads

10.

Does metoclopramide impair anastomotic healing of the left colon of rats?
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Silva, Silvana Marques e

; Ferreira, Vânia Maria Moraes

; Feres, Omar

; Oliveira, Paulo Gonçalves de

; Sousa, João Batista de

Acta Cirúrgica Brasileira

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aug 2011, Volume 26 N. 4 Pages 297 - 302

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Avaliar os efeitos da metoclopramida sobre a formação de aderências e a cicatrização de anastomoses de cólon esquerdo de ratos. MÉTODOS: 40 ratos distribuídos em dois grupos contendo 20 animais, para administração de metoclopramida (grupo experimental - E) ou solução de NaCl 0,9% (grupo controle - C). Cada grupo foi dividido em subgrupos contendo 10 animais, para eutanásia no terceiro (E3 e C3) ou sétimo dia (E7 e C7) de pós-operatório. Os ratos foram submetidos à secção do cólon esquerdo e anastomose término-terminal. No dia da re-laparotomia foi avaliada a quantidade total de aderências e removido um segmento colônico contendo a anastomose para análise da força de ruptura e concentração de hidroxiprolina. RESULTADOS: Não houve mortes ou deiscências no 3º dia de pós-operatório. No grupo E7 ocorreram uma morte e uma deiscência de anastomose bloqueada. Não houve diferença significativa entre os grupos em relação à evolução clínica, quantidade de aderências intra-cavitárias ou à anastomose e resistência tênsil no 3º ou 7º pós-operatório. A concentração de hidroxiprolina foi maior no grupo metoclopramida no 3º (p=0,006) mas não no 7º dia de pós-operatório (p=0,241) CONCLUSÃO: A metoclopramida não apresenta efeito deletério sobre a cicatrização de anastomoses intestinais em ratos.

PURPOSE: To evaluate the effects of metoclopramide on the formation of adhesion and the healing of left colonic anastomoses in rats. METHODS: Forty rats underwent sectioning of the left colon and end-to-end anastomosis and were divided into two groups of 20 animals for the administration of metoclopramide (experimental group - E) or saline solution (control group - C). Each group was divided into subgroups of 10 animals each to be killed on the third (E3 and C3) or seventh postoperative day (E7 and C7). Adhesion was assessed, and a colonic segment containing the anastomosis was removed for analysis of breaking strength and hydroxyproline concentration. RESULTS: There were no deaths or dehiscence on the 3rd postoperative day. There was one death and one blocked anastomotic dehiscence in the E7 group. No significant differences between groups were found in the analysis of clinical outcome, intra-cavity adhesion, adhesion to the anastomosis or breaking strength on the 3rd and 7th postoperative day. Hydroxyproline concentration was higher in the control group on the 3rd (p=0.006) but not on the 7th postoperative day (p=0.241). CONCLUSION: Metoclopramide did not have harmful effects on the healing of intestinal anastomoses in rats.

3089 downloads

11.

Study on adhesion formation and the healing of colon anastomosis in rats with induced peritoneal sepsis
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Silva, Silvana Marques e

; Oliveira, Marcos Vinícius Melo de

; Brandão, Alexandre Malta

; Carneiro, Fabiana Pirani

; Ferreira, Vânia Maria Moraes

; Parra, Rogério Serafim

; Feres, Omar

; Sousa, João Batista de

Acta Cirúrgica Brasileira

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2011, Volume 26 Suppl. 2 Pages 100 - 105

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Avaliar os efeitos da sepse abdominal sobre a formação de aderências e a cicatrização de anastomoses colônicas em ratos. MÉTODOS: 40 ratos distribuídos em dois grupos contendo 20 animais, para anastomose do cólon esquerdo na presença (grupo S) ou ausência (grupo N) de indução de sepse por ligadura e punção do ceco (CLP). Cada grupo foi dividido em subgrupos para eutanásia no terceiro (N3 e S3) ou sétimo (N7 e S7) dia de pós-operatório (DPO). Foi avaliada a quantidade de aderências e removido um segmento colônico contendo a anastomose para análise histopatológica, força de ruptura, hidroxiprolina e conteúdo de colágeno tecidual. RESULTADOS: Os animais submetidos à CLP apresentaram maior quantidade de aderências intra-abdominais tanto no 3° DPO (p=0,00) quanto no 7° DPO (p=0,00). Tiveram menores valores de força de ruptura no 3° DPO (p=0,00), porém maiores valores no 7° DPO (p=0,00). Não houve diferença na variação da concentração de hidroxiprolina, conteúdo de colágeno e histopatologia. CONCLUSÕES: A infecção peritoneal desencadeada por CLP aumentou a quantidade de aderências intra-cavitárias. Houve diminuição da resistência de anastomoses cólicas no 3° DPO, com posterior aumento no 7° DPO, sem efeito sobre os outros parâmetros da cicatrização.

PURPOSE: To evaluate the effects of abdominal sepsis on adhesion formation and colon anastomosis healing in rats. METHODS: Forty rats were distributed in two groups containing 20 rats each for left colon anastomosis in the presence (Group S) or absence (Group N) of induced sepsis by cecal ligation and puncture. Each group was divided into subgroups for euthanasia on the third (N3 and S3) or seventh (N7 or S7) post-operative day. The amount of adhesions was evaluated and a segment of the colon was removed for histopathologic analysis, bursting strength assessment, hydroxyproline and the determination of tissue collagen. RESULTS: The subjects which underwent cecal ligation and puncture presented a higher amount of intra-abdominal adherences in both third (p=0,00) and seventh (p=0,00) post-operatory days. Smaller bursting strengths were found in the S3 subgroup, and greater bursting strengths were found in the S7 subgroup. There was no difference in the variations on the concentrations of hydroxyproline, tissue collagen and histopathology. CONCLUSIONS: The peritoneal infection which was developed by cecal ligation and puncture raised the amount of intra-cavitary adhesions. There was a decrease in the amount of colonic anastomosis on the third post-operatory day with a following raise on the seventh without any effects on other healing parameters.

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12.

A importância da citometria de fluxo no diagnóstico raro de mieloma mielomonocítico
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Bacal, Nydia Strachman

; Guerra, João Carlos de Campos

; Ferreira, Eurípedes

; Nozawa, Sonia Tsukasa

; Kanayama, Ruth Hissae

; Di Pietro, Daniel

; Borovik, Cleide

; Hamerschlak, Nelson

; Hungria, Vania Tietsche de Moraes

; Rosenfeld, Luiz Gastão Mange

Jornal Brasileiro de Patologia e Medicina Laboratorial

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jan 2002, Volume 38 N. 1 Pages 25 - 31

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O mieloma múltiplo consiste na proliferação de células plasmáticas. Raramente apresenta, ao diagnóstico, morfologia de células imaturas com citoplasma amplo basofílico, sugerindo quadro leucêmico inicial. O objetivo deste artigo é mostrar a importância da imunofenotipagem na elucidação destes achados morfológicos pela expressão simultânea de antígenos plasmocíticos, mielomonocíticos e de linhagem linfóide T, confirmando a hipótese diagnóstica de mieloma mielomonocítico. Apresentamos dois casos de mieloma mielomonocítico através de análise morfológica (coloração pancromática de Romanovsky), citoquímica (PAS, peroxidase, sudan black, alfanaftil acetato esterase e oil red), citogenética e imunofenotipagem por citometria de fluxo em sangue periférico e medula óssea, de acordo com as técnicas recomendadas. Foram utilizados os anticorpos monoclonais: CD2, CD3, CD4, CD5, CD7, CD10, CD13, CD15, CD19, CD20, CD25, CD33, CD34, CD38, CD45, CD56, CD71, HLA-DR, TCR alfa/beta, TCR gama/delta, kappa, lambda, IgM e IgD de superfície e intracitoplasmática, assim como MPO, CD79a e CD3 intracitoplasmático. Utilizamos as técnicas de banda G e FISH nas análises citogenéticas. Foram observadas alterações clonais em ambos os casos, sendo uma com trissomia do cromossomo 8 e outro caso com deleção do braço longo do cromossomo 7 e do braço curto do cromossomo 6. Os percentuais de positividade encontrados nos anticorpos monoclonais CD4, CD7, CD10, CD13, CD14, CD15, CD33, CD38 e CD56 de forte expressão, HLA-DR, TCR gama/delta, MPO e IgM intracitoplasmático no histograma de volume x complexidade e no histograma de CD45 x complexidade permitiram concluir este diagnóstico em ambos os casos, demonstrando a importância do método.

Multiple myeloma is a malignant disease caused by unregulated proliferation of mature plasma cells. Very few cases present as blast cells in the bone marrow with intense basophylic cytoplasm and monocytoid morphology, suggesting acute leukemia. This paper presents immonophenotypying in 2 cases with the diagnostic hypoteses of myelomonocytic myeloma, showing the simultaneous expression of plasmocytic, myelomonocytic and T cell antigens. Immonophenotypying was done in peripheral blood and bone marrow with classical techniques, using the following monoclonal antibodies: CD2, CD3, CD4, CD5, CD7, CD10, CD19, CD20, CD25, CD33, CD34, CD38, CD41, CD45, CD56, CD71, HLA-DR, TCR alpha/beta, DCR gama/delta, kappa, lambda, surface IGM and IGG, intracitoplasmic kappa, lambda, IGM and IGD, MPO, CD79a and intracitoplasmic CD3. Morphology was evaluated with pan-chromatic Romanovsky stain and cytochemical stains - PAS, peroxidase, Sudan Black B, alpha-naphtil acetate esterase and Oil Red O. Cytogenetic analysis was done with banding techniques and FISH. Clonal cytogenetic alterations were present in both cases, one with chromosomo 8 trysomy and the other with long arm delection of chromosome 7 and short arm delection of chromosome 6. The percentage positivity of specific markers were clean cut making the diagnosis clear.

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13.

Pesos econômicos para seleção de gado de leite
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Vercesi Filho, Anibal Eugênio

; Madalena, Fernando Enrique

; Ferreira, José Joaquim

; Penna, Vânia Maldini

Revista Brasileira de Zootecnia

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feb 2000, Volume 29 N. 1 Pages 145 - 152

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO - Os pesos econômicos para características de importância econômica dentro do "Sistema de Produção de Leite" da Fazenda Experimental Santa Rita, pertencente à EPAMIG e localizada no município de Prudente de Moraes (MG), foram calculados. Os pesos econômicos foram definidos como a derivada parcial do lucro com respeito a cada característica (1) e como a derivada parcial da razão receita/despesa com respeito a cada característica (2), avaliadas no valor médio das outras características. Os pesos econômicos expressos em equivalente litro de leite para produção de leite com 3,1% de gordura, produção de gordura acima de 3,1%, mamite, fluxo lácteo, número de serviços por concepção, idade ao primeiro parto, vida útil e peso metabólico da vaca seca foram 31,73/kg, -23,92/kg, -3341,90/caso, 1531,26/kg/min, -1005,08/serviço, -22,30/dia, 2184,38/ano e -368,33/kg, respectivamente, para o primeiro método, e 1,52/kg, -1,76/kg, -179,50/caso, 82,23/kg/min, -53,97/serviço, -1,25/dia, 117,30/ano, -29,00/kg, multiplicados por 10-4, respectivamente, para o segundo método. Como esperado, os pesos econômicos relativos foram muito semelhantes em ambos os métodos, mas os pesos absolutos foram muito diferentes.

ABSTRACT - Economic weights for important economic characteristics in the "Milk Production System" of the Santa Rita Experimental Farm, EPAMIG, located in the municipality of Prudente de Moraes, MG were calculated. The economic weights were defined as the partial derivative of profit with respect to each trait, in method (1), and as the partial derivative of the receipts/cost ratio, in method (2), evaluated at the mean value of all other traits. The economic weights, expressed in milk equivalents, for milk yield with 3.1% fat, fat yield above 3.1%, mastitis, milk flow, services/conception, age at first calving, herdlife and dry cow metabolic weight, were respectively 31.73/kg, -23.92/kg, -3341.90/case, 1531.26/kg/min, -1005.08/service, -22.30/day, 2184.38/year and -368.33/kg for the first method (1) and 1.52/kg, -1.76/kg, -179.50/case, 82.33/kg/min, -53.97/service, -1.25/day, 117,30/year and -29.00/kg, for the second method (values multiplied by 10-4 in the latter case). As expected, the relative economic weights were similar with both methods, but the absolute weights were very different.

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