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1. Effects of sympathectomy on myocardium remodeling and function
Jordão, Maurício Rodrigues ; Pessoa, Fernanda G. ; Fonseca, Keila C.B. ; Zanoni, Fernando ; Salemi, Vera M.C. ; Souza, Leandro E. ; Ribeiro, Orlando N. ; Fernandes, Fábio ; Irigoyen, Maria Claudia ; Moreira, Luiz Felipe P. ; Mady, Charles ; Ramires, Felix Jose Alvarez .
OBJECTIVES: To evaluate the effects of sympathectomy on the myocardium in an experimental model. METHODS: The study evaluated three groups of male Wistar rats: control (CT; n=15), left unilateral sympathectomy (UNI; n=15), and bilateral sympathectomy (BIL; n=31). Sympathectomy was performed by injection of absolute alcohol into the space of the spinous process of the C7 vertebra. After 6 weeks, we assessed the chronotropic properties at rest and stress, cardiovascular autonomic modulation, myocardial and peripheral catecholamines, and beta-adrenergic receptors in the myocardium. The treadmill test consisted of an escalated protocol with a velocity increment until the maximal velocity tolerated by the animal was reached. RESULTS: The bilateral group had higher levels of peripheral catecholamines, and consequently, a higher heart rate (HR) and blood pressure levels. This suggests that the activation of a compensatory pathway in this group may have deleterious effects. The BIL group had basal tachycardia immediately before the exercise test and increased tachycardia at peak exercise (p<0.01); the blood pressure had the same pattern (p=0.0365). The variables related to autonomic modulation were not significantly different between groups, with the exception of the high frequency (HF) variable, which showed significant differences in CT vs UNI. There was no significant difference in beta receptor expression between groups. There was a higher concentration of peripheral norepinephrine in the BIL group (p=0.0001), and no significant difference in myocardial norepinephrine (p=0.09). CONCLUSION: These findings suggest that an extra cardiac compensatory pathway increases the sympathetic tonus and maintains a higher HR and higher levels of peripheral catecholamines in the procedure groups. The increase in HF activity can be interpreted as an attempt to increase the parasympathetic tonus to balance the greater sympathetic activity.
2. Effects of pasture type and level of concentrate supplementation on quality and fatty acid profile of lamb meat
Rossatti, J.A. ; Vargas, F.M. ; Retore, M. ; Britez, G.D.V. ; Silva, M.C. ; Fernandes, T. ; Fernandes, A.R.M. ; Mele, M. .
South African Journal of Animal Science 2019, Volume 49 N. 6 Pages 984 - 996
Objectives of this study were to evaluate effects of grazing tropical forage species and level of supplementation with grain on characteristics of lamb meat. Ninety-day-old lambs (n = 36) (22.54 ± 2.72 kg) were randomly assigned to a 2 x 3 factorial arrangement of pasture types (Aruana and Marandu) and levels of concentrate supplementation (0%, 1.5% and 3% of bodyweight). Water retention capacity, shear force, weight loss after cooking, pH, colour, and intramuscular lipid content of the meat were evaluated. A panel of 145 consumers evaluated the appearance, flavour, fat flavour, odour, and softness of the meat and provided an overall assessment. Supplementation at 3% of bodyweight reduced the luminosity of the meat. The appearance of meat from lambs that grazed Aruana grass was deemed preferable to that of meat from lambs that grazed Marandu grass. Total branched-chain fatty acids (BCFAs) were increased when grazing Marandu grass compared to Aruana grass. Lambs supplemented with concentrate had reduced BCFA/kg of meat and its content of both monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids were increased. Supplementation with concentrate at 1.5% and 3.0% of bodyweight increased n-6 PUFAs by 16.8% and 90.0%, decreased n-3 PUFAs by 49.7% and 35.9%, and thus increased the n-6/n-3 ratio by 135.0% and 183.8%, respectively. Lambs that were finished on grass without supplementation had a more healthful fatty acid profile and received better scores for flavour and global appreciation. To improve the quality of fatty acids in the meat, the pasture system is recommended.
3. Ativação de células de memória na produção de anticorpos e na expressão de células IgM positivas no baço de tilápias-do-nilo
Eto, S.F. ; Fernandes, D.C. ; Rosolem, M.C. ; Marinho-Neto, F.A. ; Pizauro, J.M. ; Salvador, R. ; Moraes, J.R.E. ; Moraes, F.R. .
Arquivo Brasileiro de Medicina Veterinária e Zootecnia jan 2018, Volume 70 N. 1 Pages 205 - 212
ABSTRACT This work aimed to evaluate the role of the spleen in storage and reactivation of the memory B cells, represented by IgM positive cells and the systemic production of sheep antibodies anti-red cell in Nile tilapia (Oreochromis niloticus). Two groups were established: the memory group, containing fish previously immunized with a 2,5% sheep anti-red cell, to generate the immune memory; and the naive group, containing fish that received a 0,65% saline solution. After 32 days, both groups were subjected to a new dose of the same antigen at the same concentration, volume, and inoculation via. The memory clones reactivation was correlated to the increase of the IgM positive cells in the spleen in the memory group at 0 day. The memory group showed an increase in the absolute number of lymphocytes at 21 days and an increase in the antibodies at 14 days after inoculation when compared to the naive group. The results suggest that the spleen may be a storage and reactivation place of memory B cells in Nile tilapia.
RESUMO O presente trabalho avaliou o papel do baço no armazenamento e na reativação das linhagens de células B, representadas por células IgM positivas imunomarcadas no tecido esplênico, bem como a funcionalidade dessas células, sobre a cinética dos linfócitos e na produção sistêmica de anticorpos em tilápias-do-nilo (Oreochromis niloticus). Foram separados dois grupos: grupo memória, constituído por peixes previamente imunizados com hemácia de carneiro a 2,5%, para a geração da memória imune, e o grupo naive, que recebeu o mesmo volume de solução salina a 0,65%. Após 32 dias, os dois grupos foram submetidos a uma nova dose do antígeno na mesma concentração, volume e via de inoculação. A reativação dos clones de memória foi evidenciada pelo aumento do número de células IgM positivas no baço do grupo memória no dia zero/pré-imune. Além disso, o mesmo grupo apresentou aumento dos títulos de anticorpos séricos no 14º dia e no número absoluto de linfócitos no 21º dia em relação ao grupo naive. Esses resultados sugerem que o baço não seja apenas um local de armazenamento, mas também de reativação de células B de memória em tilápia-do-nilo.
4. Implementation of immobilization accessories for positioning of small animals for radiation therapy
Arquivo Brasileiro de Medicina Veterinária e Zootecnia nov 2017, Volume 69 N. 6 Pages 1419 - 1425
ABSTRACT Radiation therapy is a modality that is presenting great advances in veterinary medicine worldwide. In Brazil, this therapeutic option is underachieved. The success of this method depends on several factors, including the use of appropriate accessories for protection and immobilization of patients. For the immobilization of small animals during treatment, in addition to sedation and anesthesia, immobilizing accessories, similar to those used in human radiotherapy, are used. This study aimed to present proposals for immobilizing accessories adapted to the positioning of small animals in order to be used in radiotherapy planning. In order to achieve results, accessories were made and tested in a living animal simulating a radiotherapy planning, which proved to be favorable to use in positioning small animals undergoing radiotherapy and for implementation processes.
RESUMO A radioterapia é uma modalidade que tem apresentando grandes avanços dentro da medicina veterinária pelo mundo. No Brasil, essa opção terapêutica é pouco realizada. O sucesso dessa modalidade depende de vários fatores, entre eles, o uso de acessórios adequados para a proteção e imobilização dos pacientes. Para a imobilização dos pequenos animais durante o tratamento, além da sedação e da anestesia, são utilizados acessórios imobilizadores semelhantes aos usados na radioterapia humana. Devido a isso, este trabalho teve como objetivo de apresentar propostas de acessórios de imobilização adaptados ao posicionamento de pequenos animais para o uso nos planejamentos radioterápicos. Para a sua realização, foram confeccionados acessórios e testados em um animal vivo simulando um planejamento radioterápico, os quais mostraram ser favoráveis ao uso nos posicionamento de pequenos animais submetidos à radioterapia e para sua implementação.
5. Brazilian guidelines for the diagnosis and treatment of postmenopausal osteoporosis
Radominski, Sebastião Cézar ; Bernardo, Wanderley ; Paula, Ana Patrícia de ; Albergaria, Ben-Hur ; Moreira, Caio ; Fernandes, Cesar Eduardo ; Castro, Charlles H.M. ; Zerbini, Cristiano Augusto de Freitas ; Domiciano, Diogo S. ; Mendonça, Laura M.C. ; Pompei, Luciano de Melo ; Bezerra, Mailze Campos ; Loures, Marco Antônio R. ; Wender, Maria Celeste Osório ; Lazaretti-Castro, Marise ; Pereira, Rosa M.R. ; Maeda, Sergio Setsuo ; Szejnfeld, Vera Lúcia ; Borba, Victoria Z.C. .
Revista Brasileira de Reumatologia 2017, Volume 57 Suppl. 2 Pages s452 - s466
Abstract Osteoporosis is the leading cause of fractures in the population older than 50 years. This silent disease affects primarily postmenopausal women and the elderly, and the morbidity and mortality rates are high. The main goal of treating osteoporosis is the prevention of fractures. The identification of populations at risk through early diagnosis and treatment is essential. The last Brazilian guideline for the treatment of postmenopausal osteoporosis was elaborated in 2002. Since then, new strategies for diagnosis and risk stratification have been developed, and drugs with novel action mechanisms have been added to the therapeutic arsenal. The Osteoporosis and Osteometabolic Diseases Committee of the Brazilian Society of Rheumatology, in conjunction with the Brazilian Medical Association and other Societies, has developed this update of the guidelines for the treatment of postmenopausal osteoporosis according to the best scientific evidence available. This update is intended for professionals in many medical and health specialties involved in the treatment of osteoporosis, for physicians in general and for health-related organizations.
Resumo A osteoporose é a principal causa de fraturas na população acima de 50 anos. É uma doença silenciosa que afeta especialmente as mulheres na pós-menopausa e idosos e tem elevada taxa de morbimortalidade. O principal objetivo do tratamento da osteoporose é a prevenção das fraturas. A identificação dessa população de risco através do diagnóstico e tratamento precoces é de fundamental importância. A última diretriz brasileira para tratamento da osteoporose em mulheres na pós-menopausa foi elaborada em 2002. Desde então foram desenvolvidas novas estratégias de diagnóstico da osteoporose, bem como fármacos com novos mecanismos de ação foram adicionados ao arsenal terapêutico. A Comissão de Osteoporose e Doenças Osteometabólicas da Sociedade Brasileira de Reumatologia em conjunto com a Associação Médica Brasileira e sociedades afins desenvolveu esta atualização da diretriz do tratamento da osteoporose em mulheres na pós-menopausa de acordo com as melhores evidências científicas disponíveis. Esta atualização é destinada aos profissionais das várias especialidades médicas e da área da saúde envolvidos no tratamento da osteoporose, médicos em geral e organizações relacionadas à saúde.
6. Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice
Carlesso, F.N. ; Fuscaldi, L.L. ; Araújo, R.S. ; Teixeira, C.S. ; Oliveira, M.C. ; Fernandes, S.O.A. ; Cassali, G.D. ; Reis, D.C. ; Barros, A.L.B. ; Cardoso, V.N. .
Brazilian Journal of Medical and Biological Research oct 2015, Volume 48 N. 10 Pages 923 - 928
Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using99mTc-HYNIC-βAla-Bombesin(7-14)as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods showed higher uptake by pancreatic tumor when compared to muscle (used as control), and the tumor-to-muscle ratio indicated that99mTc-HYNIC-βAla-Bombesin(7-14)uptake was four-fold higher in tumor cells than in other tissues. Scintigraphic images also showed a clear signal at the tumor site. The present data indicate that99mTc-HYNIC-βAla-Bombesin(7-14)may be useful for the detection of pancreatic adenocarcinoma.
7. Interspecific transmission of small ruminant lentiviruses from goats to sheep
Souza, Thiago S. de ; Pinheiro, Raymundo R. ; Costa, Joselito N. ; Lima, Carla C.V. de ; Andrioli, Alice ; Azevedo, Dalva A.A. de ; Santos, Vanderlan W.S. dos ; Araújo, Juscilânia F. ; Sousa, Ana Lídia M. de ; Pinheiro, Danielle N.S. ; Fernandes, Flora M.C. ; Costa Neto, Antonio O. .
Brazilian Journal of Microbiology sep 2015, Volume 46 N. 3 Pages 867 - 874
<p>This study was conducted in order to evaluate the transmission of caprine lentivirus to sheep using different experimental groups. The first one (colostrum group) was formed by nine lambs receiving colostrum from goats positive for small ruminant lentiviruses (SRLV). The second group (milk group) was established by nine lambs that received milk of these goats. Third was a control group, consisting of lambs that suckled colostrum and milk of negative mothers. Another experimental group (contact group) was formed by eight adult sheep, confined with two naturally infected goats. The groups were monitored by immunoblotting (IB), enzyme-linked immunosorbent assay (ELISA), agar gel immunodiffusion (AGID) and nested polymerase chain reaction (nPCR). All lambs that suckled colostrum and milk of infected goats and six sheep of the contact group had positive results in the nPCR, although seroconversion was detected only in three of the exposed animals, with no clinical lentiviruses manifestation, in 720 days of observation. There was a close relationship between viral sequences obtained from infected animals and the prototype CAEV-Cork. Thus, it was concluded that SRLV can be transmitted from goats to sheep, however, the degree of adaptation of the virus strain to the host species probably interferes with the infection persistence and seroconversion rate.</p>
8. Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers
Mori, Enio ; Lara, Maria do Carmo C.S.H. ; Cunha, Elenice M.S. ; Villalobos, Eliana M.C. ; Mori, Claudia M.C. ; Soares, Rodrigo M. ; Brandão, Paulo E. ; Fernandes, Wilson R. ; Richtzenhain, Leonardo J. .
Brazilian Journal of Microbiology jun 2015, Volume 46 N. 2 Pages 565 - 570
Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood, cerebrospinal fluid (CSF) and nasal swabs of specimens from a total of 64 animals. Only one CSF sample (Iso07/05 strain) was positive by virus isolation in cell culture. EHV-1 Iso07/05 neurologic strain and two abortion visceral tissues samples (Iso11/06 and Iso33/06) were PCR-positive for ORF33 (glycoprotein B, gB) gene of EHV-1. A sequence analysis of the ORF72, ORF64 and ORF30 genes from three EHV-1 archival strains (A3/97, A4/72, A9/92) and three clinical samples (Iso07/05, Iso11/06 and Iso33/06) suggested that among Brazilian EHV-1 strains, the amplified region of the gD gene sequence is highly conserved. Additionally, the analysis of ICP4 gene showed high nucleotide and amino acid identities when compared with genotype P strains, suggesting that the EHV-1 Brazilian strains belonged to the same group. All the EHV-1 Brazilian strains were classified as non-neuropathogenic variants (N752) based on the ORF30 analysis. These findings indicate a high conservation of the gD-, ICP4- and ORF30-encoding sequences. Different pathotypes of the EHV-1 strain might share identical genes with no specific markers, and tissue tropism is not completely dependent on the gD envelope, immediate-early ICP4 and DNA polymerase proteins.
9. Growing knowledge: an overview of Seed Plant diversity in Brazil
Zappi, Daniela C. ; Filardi, Fabiana L. Ranzato ; Leitman, Paula ; Souza, Vinícius C. ; Walter, Bruno M.T. ; Pirani, José R. ; Morim, Marli P. ; Queiroz, Luciano P. ; Cavalcanti, Taciana B. ; Mansano, Vidal F. ; Forzza, Rafaela C. ; Abreu, Maria C. ; Acevedo-Rodríguez, Pedro ; Agra, Maria F. ; Almeida Jr., Eduardo B. ; Almeida, Gracineide S.S. ; Almeida, Rafael F. ; Alves, Flávio M. ; Alves, Marccus ; Alves-Araujo, Anderson ; Amaral, Maria C.E. ; Amorim, André M. ; Amorim, Bruno ; Andrade, Ivanilza M. ; Andreata, Regina H.P. ; Andrino, Caroline O. ; Anunciação, Elisete A. ; Aona, Lidyanne Y.S. ; Aranguren, Yani ; Aranha Filho, João L.M. ; Araújo, Andrea O. ; Araújo, Ariclenes A.M. ; Araújo, Diogo ; Arbo, María M. ; Assis, Leandro ; Assis, Marta C. ; Assunção, Vivian A. ; Athiê-Souza, Sarah M. ; Azevedo, Cecilia O. ; Baitello, João B. ; Barberena, Felipe F.V.A. ; Barbosa, Maria R.V. ; Barros, Fábio ; Barros, Lucas A.V. ; Barros, Michel J.F. ; Baumgratz, José F.A. ; Bernacci, Luis C. ; Berry, Paul E. ; Bigio, Narcísio C. ; Biral, Leonardo ; Bittrich, Volker ; Borges, Rafael A.X. ; Bortoluzzi, Roseli L.C. ; Bove, Cláudia P. ; Bovini, Massimo G. ; Braga, João M.A. ; Braz, Denise M. ; Bringel Jr., João B.A. ; Bruniera, Carla P. ; Buturi, Camila V. ; Cabral, Elza ; Cabral, Fernanda N. ; Caddah, Mayara K. ; Caires, Claudenir S. ; Calazans, Luana S.B. ; Calió, Maria F. ; Camargo, Rodrigo A. ; Campbell, Lisa ; Canto-Dorow, Thais S. ; Carauta, Jorge P.P. ; Cardiel, José M. ; Cardoso, Domingos B.O.S. ; Cardoso, Leandro J.T. ; Carneiro, Camila R. ; Carneiro, Cláudia E. ; Carneiro-Torres, Daniela S. ; Carrijo, Tatiana T. ; Caruzo, Maria B.R. ; Carvalho, Maria L.S. ; Carvalho-Silva, Micheline ; Castello, Ana C.D. ; Cavalheiro, Larissa ; Cervi, Armando C. ; Chacon, Roberta G. ; Chautems, Alain ; Chiavegatto, Berenice ; Chukr, Nádia S. ; Coelho, Alexa A.O.P. ; Coelho, Marcus A.N. ; Coelho, Rubens L.G. ; Cordeiro, Inês ; Cordula, Elizabeth ; Cornejo, Xavier ; Côrtes, Ana L.A. ; Costa, Andrea F. ; Costa, Fabiane N. ; Costa, Jorge A.S. ; Costa, Leila C. ; Costa-e-Silva, Maria B. ; Costa-Lima, James L. ; Cota, Maria R.C. ; Couto, Ricardo S. ; Daly, Douglas C. ; De Stefano, Rodrigo D. ; De Toni, Karen ; Dematteis, Massimiliano ; Dettke, Greta A. ; Di Maio, Fernando R. ; Dórea, Marcos C. ; Duarte, Marília C. ; Dutilh, Julie H.A. ; Dutra, Valquíria F. ; Echternacht, Lívia ; Eggers, Lilian ; Esteves, Gerleni ; Ezcurra, Cecilia ; Falcão Junior, Marcus J.A. ; Feres, Fabíola ; Fernandes, José M. ; Ferreira, D.M.C. ; Ferreira, Fabrício M. ; Ferreira, Gabriel E. ; Ferreira, Priscila P.A. ; Ferreira, Silvana C. ; Ferrucci, Maria S. ; Fiaschi, Pedro ; Filgueiras, Tarciso S. ; Firens, Marcela ; Flores, Andreia S. ; Forero, Enrique ; Forster, Wellington ; Fortuna-Perez, Ana P. ; Fortunato, Reneé H. ; Fraga, Cléudio N. ; França, Flávio ; Francener, Augusto ; Freitas, Joelcio ; Freitas, Maria F. ; Fritsch, Peter W. ; Furtado, Samyra G. ; Gaglioti, André L. ; Garcia, Flávia C.P. ; Germano Filho, Pedro ; Giacomin, Leandro ; Gil, André S.B. ; Giulietti, Ana M. ; A.P.Godoy, Silvana ; Goldenberg, Renato ; Gomes da Costa, Géssica A. ; Gomes, Mário ; Gomes-Klein, Vera L. ; Gonçalves, Eduardo Gomes ; Graham, Shirley ; Groppo, Milton ; Guedes, Juliana S. ; Guimarães, Leonardo R.S. ; Guimarães, Paulo J.F. ; Guimarães, Elsie F. ; Gutierrez, Raul ; Harley, Raymond ; Hassemer, Gustavo ; Hattori, Eric K.O. ; Hefler, Sonia M. ; Heiden, Gustavo ; Henderson, Andrew ; Hensold, Nancy ; Hiepko, Paul ; Holanda, Ana S.S. ; Iganci, João R.V. ; Imig, Daniela C. ; Indriunas, Alexandre ; Jacques, Eliane L. ; Jardim, Jomar G. ; Kamer, Hiltje M. ; Kameyama, Cíntia ; Kinoshita, Luiza S. ; Kirizawa, Mizué ; Klitgaard, Bente B. ; Koch, Ingrid ; Koschnitzke, Cristiana ; Krauss, Nathália P. ; Kriebel, Ricardo ; Kuntz, Juliana ; Larocca, João ; Leal, Eduardo S. ; Lewis, Gwilym P. ; Lima, Carla T. ; Lima, Haroldo C. ; Lima, Itamar B. ; Lima, Laíce F.G. ; Lima, Laura C.P. ; Lima, Leticia R. ; Lima, Luís F.P. ; Lima, Rita B. ; Lírio, Elton J. ; Liro, Renata M. ; Lleras, Eduardo ; Lobão, Adriana ; Loeuille, Benoit ; Lohmann, Lúcia G. ; Loiola, Maria I.B. ; Lombardi, Julio A. ; Longhi-Wagner, Hilda M. ; Lopes, Rosana C. ; Lorencini, Tiago S. ; Louzada, Rafael B. ; Lovo, Juliana ; Lozano, Eduardo D. ; Lucas, Eve ; Ludtke, Raquel ; Luz, Christian L. ; Maas, Paul ; Machado, Anderson F.P. ; Macias, Leila ; Maciel, Jefferson R. ; Magenta, Mara A.G. ; Mamede, Maria C.H. ; Manoel, Evelin A. ; Marchioretto, Maria S. ; Marques, Juliana S. ; Marquete, Nilda ; Marquete, Ronaldo ; Martinelli, Gustavo ; Martins da Silva, Regina C.V. ; Martins, Ângela B. ; Martins, Erika R. ; Martins, Márcio L.L. ; Martins, Milena V. ; Martins, Renata C. ; Matias, Ligia Q. ; Maya-L., Carlos A. ; Mayo, Simon ; Mazine, Fiorella ; Medeiros, Debora ; Medeiros, Erika S. ; Medeiros, Herison ; Medeiros, João D. ; Meireles, José E. ; Mello-Silva, Renato ; Melo, Aline ; Melo, André L. ; Melo, Efigênia ; Melo, José I.M. ; Menezes, Cristine G. ; Menini Neto, Luiz ; Mentz, Lilian A. ; Mezzonato, A.C. ; Michelangeli, Fabián A. ; Milward-de-Azevedo, Michaele A. ; Miotto, Silvia T.S. ; Miranda, Vitor F.O. ; Mondin, Cláudio A. ; Monge, Marcelo ; Monteiro, Daniele ; Monteiro, Raquel F. ; Moraes, Marta D. ; Moraes, Pedro L.R. ; Mori, Scott A. ; Mota, Aline C. ; Mota, Nara F.O. ; Moura, Tania M. ; Mulgura, Maria ; Nakajima, Jimi N. ; Nardy, Camila ; Nascimento Júnior, José E. ; Noblick, Larry ; Nunes, Teonildes S. ; O'Leary, Nataly ; Oliveira, Arline S. ; Oliveira, Caetano T. ; Oliveira, Juliana A. ; Oliveira, Luciana S.D. ; Oliveira, Maria L.A.A. ; Oliveira, Regina C. ; Oliveira, Renata S. ; Oliveira, Reyjane P. ; Paixão-Souza, Bruno ; Parra, Lara R. ; Pasini, Eduardo ; Pastore, José F.B. ; Pastore, Mayara ; Paula-Souza, Juliana ; Pederneiras, Leandro C. ; Peixoto, Ariane L. ; Pelissari, Gisela ; Pellegrini, Marco O.O. ; Pennington, Toby ; Perdiz, Ricardo O. ; Pereira, Anna C.M. ; Pereira, Maria S. ; Pereira, Rodrigo A.S. ; Pessoa, Clenia ; Pessoa, Edlley M. ; Pessoa, Maria C.R. ; Pinto, Luiz J.S. ; Pinto, Rafael B. ; Pontes, Tiago A. ; Prance, Ghillean T. ; Proença, Carolyn ; Profice, Sheila R. ; Pscheidt, Allan C. ; Queiroz, George A. ; Queiroz, Rubens T. ; Quinet, Alexandre ; Rainer, Heimo ; Ramos, Eliana ; Rando, Juliana G. ; Rapini, Alessandro ; Reginato, Marcelo ; Reis, Ilka P. ; Reis, Priscila A. ; Ribeiro, André R.O. ; Ribeiro, José E.L.S. ; Riina, Ricarda ; Ritter, Mara R. ; Rivadavia, Fernando ; Rocha, Antônio E.S. ; Rocha, Maria J.R. ; Rodrigues, Izabella M.C. ; Rodrigues, Karina F. ; Rodrigues, Rodrigo S. ; Rodrigues, Rodrigo S. ; Rodrigues, Vinícius T. ; Rodrigues, William ; Romaniuc Neto, Sérgio ; Romão, Gerson O. ; Romero, Rosana ; Roque, Nádia ; Rosa, Patrícia ; Rossi, Lúcia ; Sá, Cyl F.C. ; Saavedra, Mariana M. ; Saka, Mariana ; Sakuragui, Cássia M. ; Salas, Roberto M. ; Sales, Margareth F. ; Salimena, Fatima R.G. ; Sampaio, Daniela ; Sancho, Gisela ; Sano, Paulo T. ; Santos, Alessandra ; Santos, Élide P. ; Santos, Juliana S. ; Santos, Marianna R. ; Santos-Gonçalves, Ana P. ; Santos-Silva, Fernanda ; São-Mateus, Wallace ; Saraiva, Deisy P. ; Saridakis, Dennis P. ; Sartori, Ângela L.B. ; Scalon, Viviane R. ; Schneider, Ângelo ; Sebastiani, Renata ; Secco, Ricardo S. ; Senna, Luisa ; Senna-Valle, Luci ; Shirasuna, Regina T. ; Silva Filho, Pedro J.S. ; Silva, Anádria S. ; Silva, Christian ; Silva, Genilson A.R. ; Silva, Gisele O. ; Silva, Márcia C.R. ; Silva, Marcos J. ; Silva, Marcos J. ; Silva, Otávio L.M. ; Silva, Rafaela A.P. ; Silva, Saura R. ; Silva, Tania R.S. ; Silva-Gonçalves, Kelly C. ; Silva-Luz, Cíntia L. ; Simão-Bianchini, Rosângela ; Simões, André O. ; Simpson, Beryl ; Siniscalchi, Carolina M. ; Siqueira Filho, José A. ; Siqueira, Carlos E. ; Siqueira, Josafá C. ; Smith, Nathan P. ; Snak, Cristiane ; Soares Neto, Raimundo L. ; Soares, Kelen P. ; Soares, Marcos V.B. ; Soares, Maria L. ; Soares, Polyana N. ; Sobral, Marcos ; Sodré, Rodolfo C. ; Somner, Genise V. ; Sothers, Cynthia A. ; Sousa, Danilo J.L. ; Souza, Elnatan B. ; Souza, Élvia R. ; Souza, Marcelo ; Souza, Maria L.D.R. ; Souza-Buturi, Fátima O. ; Spina, Andréa P. ; Stapf, María N.S. ; Stefano, Marina V. ; Stehmann, João R. ; Steinmann, Victor ; Takeuchi, Cátia ; Taylor, Charlotte M. ; Taylor, Nigel P. ; Teles, Aristônio M. ; Temponi, Lívia G. ; Terra-Araujo, Mário H. ; Thode, Veronica ; Thomas, W.Wayt ; Tissot-Squalli, Mara L. ; Torke, Benjamin M. ; Torres, Roseli B. ; Tozzi, Ana M.G.A. ; Trad, Rafaela J. ; Trevisan, Rafael ; Trovó, Marcelo ; Valls, José F.M. ; Vaz, Angela M.S.F. ; Versieux, Leonardo ; Viana, Pedro L. ; Vianna Filho, Marcelo D.M. ; Vieira, Ana O.S. ; Vieira, Diego D. ; Vignoli-Silva, Márcia ; Vilar, Thaisa ; Vinhos, Franklin ; Wallnöfer, Bruno ; Wanderley, Maria G.L. ; Wasshausen, Dieter ; Watanabe, Maurício T.C. ; Weigend, Maximilian ; Welker, Cassiano A.D. ; Woodgyer, Elizabeth ; Xifreda, Cecilia C. ; Yamamoto, Kikyo ; Zanin, Ana ; Zenni, Rafael D. ; Zickel, Carmem S .
Rodriguésia 2015, Volume 66 N. 4 Pages 1085 - 1113
Abstract An updated inventory of Brazilian seed plants is presented and offers important insights into the country's biodiversity. This work started in 2010, with the publication of the Plants and Fungi Catalogue, and has been updated since by more than 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing an increase of 3% in its species richness in relation to 2010. The Amazon Rainforest is the richest Brazilian biome for Gymnosperms, while the Atlantic Rainforest is the richest one for Angiosperms. There was a considerable increment in the number of species and endemism rates for biomes, except for the Amazon that showed a decrease of 2.5% of recorded endemics. However, well over half of Brazillian seed plant species (57.4%) is endemic to this territory. The proportion of life-forms varies among different biomes: trees are more expressive in the Amazon and Atlantic Rainforest biomes while herbs predominate in the Pampa, and lianas are more expressive in the Amazon, Atlantic Rainforest, and Pantanal. This compilation serves not only to quantify Brazilian biodiversity, but also to highlight areas where there information is lacking and to provide a framework for the challenge faced in conserving Brazil's unique and diverse flora.
Resumo Um levantamento atualizado das plantas com sementes e análises relevantes acerca desta biodiversidade são apresentados. Este trabalho se iniciou em 2010 com a publicação do Catálogo de Plantas e Fungos e, desde então vem sendo atualizado por mais de 430 especialistas trabalhando online. O Brasil abriga atualmente 32.086 espécies nativas de Angiospermas e 23 espécies nativas de Gimnospermas e estes novos dados mostram um aumento de 3% da riqueza em relação a 2010. A Amazônia é o Domínio Fitogeográfico com o maior número de espécies de Gimnospermas, enquanto que a Floresta Atlântica possui a maior riqueza de Angiospermas. Houve um crescimento considerável no número de espécies e nas taxas de endemismo para a maioria dos Domínios (Caatinga, Cerrado, Floresta Atlântica, Pampa e Pantanal), com exceção da Amazônia que apresentou uma diminuição de 2,5% de endemicidade. Entretanto, a maior parte das plantas com sementes que ocorrem no Brasil (57,4%) é endêmica deste território. A proporção de formas de vida varia de acordo com os diferentes Domínios: árvores são mais expressivas na Amazônia e Floresta Atlântica do que nos outros biomas, ervas são dominantes no Pampa e as lianas apresentam riqueza expressiva na Amazônia, Floresta Atlântica e Pantanal. Este trabalho não só quantifica a biodiversidade brasileira, mas também indica as lacunas de conhecimento e o desafio a ser enfrentado para a conservação desta flora.
10. Pleiotropic effects of simvastatin in physically trained ovariectomized rats
Bernardes, N. ; Brito, J.O. ; Fernandes, T.G. ; Llesuy, S.F. ; Irigoyen, M.C. ; Belló-Klein, A. ; De Angelis, K. .
Brazilian Journal of Medical and Biological Research may 2013, Volume 46 N. 5 Pages 447 - 453
This study tested the hypothesis that simvastatin treatment can improve cardiovascular and autonomic functions and membrane lipoperoxidation, with an increased effect when applied to physically trained ovariectomized rats. Ovariectomized rats were divided into sedentary, sedentary+simvastatin and trained+simvastatin groups (n = 8 each). Exercise training was performed on a treadmill for 8 weeks and simvastatin (5 mg/kg) was administered in the last 2 weeks. Blood pressure (BP) was recorded in conscious animals. Baroreflex sensitivity was evaluated by the tachycardic and bradycardic responses to BP changes. Cardiac vagal and sympathetic effects were determined using methylatropine and propranolol. Oxidative stress was evaluated based on heart and liver lipoperoxidation using the chemiluminescence method. The simvastatin-treated groups presented reduced body weight and mean BP (trained+simvastatin = 99 ± 2 and sedentary+simvastatin = 107 ± 2 mmHg) compared to the sedentary group (122 ± 1 mmHg). Furthermore, the trained group showed lower BP and heart rate compared to the other groups. Tachycardic and bradycardic responses were enhanced in both simvastatin-treated groups. The vagal effect was increased in the trained+simvastatin group and the sympathetic effect was decreased in the sedentary+simvastatin group. Hepatic lipoperoxidation was reduced in sedentary+simvastatin (≈21%) and trained+simvastatin groups (≈57%) compared to the sedentary group. Correlation analysis involving all animals demonstrated that cardiac lipoperoxidation was negatively related to the vagal effect (r = -0.7) and positively correlated to the sympathetic effect (r = 0.7). In conclusion, improvement in cardiovascular and autonomic functions associated with a reduction of lipoperoxidation with simvastatin treatment was increased in trained ovariectomized rats.
11. Identification of pathogens and virulence profile of Rhodococcus equi and Escherichia coli strains obtained from sand of parks
Fernandes, M.C. ; Takai, S. ; Leite, D.S. ; Pinto, J.P.A.N. ; Brandão, P.E. ; Santarém, V.A. ; Listoni, F.J.P. ; Silva, A.V. Da ; Ribeiro, M.G. .
Brazilian Journal of Microbiology 2013, Volume 44 N. 2 Pages 485 - 492
The identification of pathogens of viral (Rotavirus, Coronavirus), parasitic (Toxocara spp.) and bacterial (Escherichia coli, Salmonella spp., Rhodococcus equi) origin shed in feces, and the virulence profile of R. equi and E. coli isolates were investigated in 200 samples of sand obtained from 40 parks, located in central region of state of Sao Paulo, Brazil, using different diagnostic methods. From 200 samples analyzed, 23 (11.5%) strains of R. equi were isolated. None of the R. equi isolates showed a virulent (vapA gene) or intermediately virulent (vapB gene) profiles. Sixty-three (31.5%) strains of E. coli were identified. The following genes encoding virulence factors were identified in E. coli: eae, bfp, saa, iucD, papGI, sfa and hly. Phylogenetic classification showed that 63 E. coli isolates belonged to groups B1 (52.4%), A (25.4%) and B2 (22.2%). No E. coli serotype O157:H7 was identified. Eggs of Toxocara sp. were found in three parks and genetic material of bovine Coronavirus was identified in one sample of one park. No Salmonella spp. and Rotavirus isolates were identified in the samples of sand. The presence of R. equi, Toxocara sp, bovine Coronavirus and virulent E. coli isolates in the environment of parks indicates that the sanitary conditions of the sand should be improved in order to reduce the risks of fecal transmission of pathogens of zoonotic potential to humans in these places.
12. Mastite bovina por Pasteurella multocida: estudo de nove casos
Ribeiro, M.G. ; Lara, G.H.B. ; Fernandes, M.C. ; Paes, A.C. ; Motta, R.G. ; Siqueira, A.K. ; Salerno, T. ; Listoni, F.J.P. .
Arquivo Brasileiro de Medicina Veterinária e Zootecnia aug 2010, Volume 62 N. 4 Pages 985 - 988
https://doi.org/10.1590/S0102-09352010000400031 4617 downloads
13. Effect of carotid and aortic baroreceptors on cardiopulmonary reflex: the role of autonomic function
Fernandes, T.L. ; Piratello, A.C. ; Farah, V. ; Fiorino, P. ; Moreira, E.D. ; Irigoyen, M.C. ; Krieger, E.M. .
Brazilian Journal of Medical and Biological Research jul 2010, Volume 43 N. 7 Pages 681 - 686
We determined the sympathetic and parasympathetic control of heart rate (HR) and the sensitivity of the cardiopulmonary receptors after selective carotid and aortic denervation. We also investigated the participation of the autonomic nervous system in the Bezold-Jarish reflex after selective removal of aortic and carotid baroreceptors. Male Wistar rats (220-270 g) were divided into three groups: control (CG, N = 8), aortic denervation (AG, N = 5) and carotid denervation (CAG, N = 9). AG animals presented increased arterial pressure (12%) and HR (11%) compared with CG, while CAG animals presented a reduction in arterial pressure (16%) and unchanged HR compared with CG. The sequential blockade of autonomic effects by atropine and propranolol indicated a reduction in vagal function in CAG (a 50 and 62% reduction in vagal effect and tonus, respectively) while AG showed an increase of more than 100% in sympathetic control of HR. The Bezold-Jarish reflex was evaluated using serotonin, which induced increased bradycardia and hypotension in AG and CAG, suggesting that the sensitivity of the cardiopulmonary reflex is augmented after selective denervation. Atropine administration abolished the bradycardic responses induced by serotonin in all groups; however, the hypotensive response was still increased in AG. Although the responses after atropine were lower than the responses before the drug, indicating a reduction in vagal outflow after selective denervation, our data suggest that both denervation procedures are associated with an increase in sympathetic modulation of the vessels, indicating that the sensitivity of the cardiopulmonary receptors was modulated by baroreceptor fibers.
https://doi.org/10.1590/S0100-879X2010000700011 2301 downloads
14. Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil
Sousa-Junior, F.C. de ; Silva-Carvalho, M.C. ; Fernandes, M.J.B.C. ; Vieira, M.F.P. ; Pellegrino, F.L.P.C. ; Figueiredo, A.M.S. ; Melo, M.C.N. de ; Milan, E.P. .
Brazilian Journal of Medical and Biological Research oct 2009, Volume 42 N. 10 Pages 877 - 881
Methicillin-resistant Staphylococcus aureus (MRSA) is a major agent of hospital infections worldwide. In Brazil, a multiresistant MRSA lineage (ST239-SCCmecIIIA), the so-called Brazilian epidemic clone (BEC), has predominated in all regions. However, an increase in nosocomial infections caused by non-multiresistant MRSA clones has recently been observed. In the present study, 45 clinical isolates of MRSA obtained from a university hospital located in Natal city, Brazil, were identified by standard laboratory methods and molecularly characterized using staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was carried out using CLSI methods. The MRSA isolates studied displayed a total of 8 different pulsed-field gel electrophoresis patterns (types A to H) with predominance (73%) of pattern A (BEC-related). However, MRSA harboring SCCmec type IV were also identified, 3 (7%) of which were genetically related to the pediatric clone - USA800 (ST5-SCCmecIV). In addition, we found a considerable genetic diversity within BEC isolates. MRSA displaying SCCmecIV are frequently susceptible to the majority of non-β-lactam antibiotics. However, emergence of multiresistant variants of USA800 was detected.
https://doi.org/10.1590/S0100-879X2009005000018 2059 downloads
15. LINFADENITE INFECCIOSA EM SUÍNOS: ETIOLOGIA, EPIDEMIOLOGIA E ASPECTOS EM SAÚDE PÚBLICA
Arquivos do Instituto Biológico jun 2009, Volume 76 N. 2 Pages 317 - 325
ABSTRACT Infectious lymphadenitis in swine leads to high damages with losses of carcasses, due to similarity of lesions caused by causal agents and zoonotic risks of certain microorganisms, reinforcing the importance of periodic sanitary vigilance in affections of lymph nodes in swine. The increased occurrence of Mycobacterium sp. and Rhodococcus equi in patients affected by acquired immunodeficiency syndrome (AIDS) has underscored the importance of the monitoring of infectious lymphadenitis in slaughterhouses, as these microorganisms are among the most common causes of swine lymphadenitis. The present article reviewed the main aspects of lymphadenitis in swine, with emphasis on the epidemiology and public health impact, and Mycobacterium and Rhodococcus equi as causal agents.
RESUMO A linfadenite infecciosa em suínos gera altos prejuízos com a condenação de carcaças, pela similaridade das lesões causadas pelos agentes causais e de certos micro-organismos, reforçando a necessidade da vigilância sanitária continuada nas afecções de linfonodos em suínos. O crescente isolamento de Mycobacterium sp. e de Rhodococcus equi em pacientes acometidos pela síndrome da imunodeficiência adquirida (Aids) redobrou a preocupação na vigilância da linfadenite na linha de abate, em virtude destes micro-organismos figurarem dentre as principais causas da linfadenite em suínos. O presente artigo revisou os principais aspectos da linfadenite infecciosa em suínos, com ênfase na epidemiologia e reflexos em saúde pública, e a ocorrência de Mycobacterium sp. e R. equi como agentes causais da linfadenite.
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