The objectives of this study were to determine the chemical composition of vegetable and mixed crude glycerin and digestible energy to and evaluate different inclusion levels in rabbit feeding. A total of 108 rabbits were used in the digestibility assay, assigned in a completely randomized design into nine treatments, one reference diet plus eight test diets, where the glycerin types were included at levels of 4, 8, 12 and 16%, replacing the reference diet roughage. In the performance assay, 180 rabbits were assigned to a completely randomized design into a 2 × 4 factorial arrangement (two types of glycerin and four levels of inclusion: 3, 6, 9 and 12%) plus a reference diet and ten replications. The vegetable and mixed crude glycerin showed digestible energy of 5,099 and 4,953 kcal/kg of dry matter, respectively. Although feed intake decreased linearly with the growing inclusion of both kinds of glycerin during both evaluated periods (from 32 to 50 and 32 to 70 days), performance losses occurred only with the inclusion of vegetable crude glycerin. The inclusion of mixed crude glycerin up to 12% allowed similar animal performance to the reference diet up to 50 days, unlike vegetable crude glycerin, for which inclusions over 6% in the diet resulted in poorer performance compared with that obtained with the reference diet. In the total period, differences were not observed for live weight or weight gain of the animals fed both glycerin types. Carcass weight decreased linearly with the inclusion of both glycerin types, with lower values compared with the reference diet only for the two highest levels of glycerin inclusion. Mixed crude glycerin can be included up to the maximum level studied (12%), while vegetable glycerin can be added up to 6% in the diet of growing rabbits.