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Prospection of sustainable agrifood systems based on discourse analysis of Curraleiro Pé-Duro cattle breeders in Brazil
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Silva, M.C.

; Santos, H.D.

; Frank, L.E.B.

; Ferraz, J.B.S.

; Schlindwein, M.M.

; Garcia, J.A.S.

; Souza, A.B.B.

; Ferreira, J.L.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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mar 2023, Volume 75 N. 2 Pages 267 - 279

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Developing countries face great challenges to couple agricultural intensification and sustainable supply chain development. This paper explores the opportunities for innovation in the conservation of the locally adapted Curraleiro Pé-Duro (CPD) cattle. The CPD is a historical Brazilian breed, related to regional culture, which exhibits remarkable adaptability in tropical biomes. The research method consisted of interviewing and analyzing the discourse of CPD breeders in Tocantins, Brazil. The systematization of the data resulted in four categories and eleven subcategories, which were defined a posteriori. Our findings reveal motivational aspects, potentialities, and prospects for valuation, besides the needs and perceived challenges faced by CPD farmers. A better understanding of the current situation may guide public policies, encourage stakeholders, benefit farmers, and lead to sustainable agri-food initiatives. The empirical contributions of our diagnosis are discussed, and we show how they bring forward and provide valuable lessons to prospect innovations in the conservation of CPD cattle. These findings may support scholars and people who are also interested in accessing and supporting the conservation of indigenous livestock. In general, the study supports the definition of strategies, planning, and investments for the value chain, including the participation of upstream and downstream segments of the farms.

RESUMO Os países em desenvolvimento enfrentam grandes desafios para combinar a intensificação da agricultura com o desenvolvimento sustentável das cadeias de abastecimento. Este documento explora as oportunidades de inovação na conservação do gado Curraleiro Pé-Duro (CPD). O CPD é uma raça brasileira histórica, relacionada à cultura regional, que apresenta notável adaptabilidade em biomas tropicais. O método de pesquisa consistiu em entrevistar e analisar o discurso dos criadores de CPD no Tocantins, Brasil. A sistematização dos dados resultou em quatro categorias e onze subcategorias, que foram definidas a posteriori. Nossas descobertas revelam aspectos motivacionais, potencialidades e perspectivas de valorização, além das necessidades e desafios percebidos pelos produtores de CPD. Uma melhor compreensão da situação atual pode orientar as políticas públicas, incentivar os interessados, beneficiar os agricultores e levar a iniciativas agroalimentares sustentáveis. As contribuições empíricas de nosso diagnóstico são discutidas e mostramos como elas trazem e fornecem lições valiosas para prospectar inovações na conservação do gado de CPD. Estas descobertas podem apoiar acadêmicos e pessoas que também estão interessadas em acessar e apoiar a conservação e produção animal com uso de raças crioulas. Em geral, o estudo apoia a definição de estratégias, planejamento e investimentos relacionados com cadeia de valor, incluindo a participação de segmentos a montante e a jusante das fazendas.

https://doi.org/10.1590/1678-4162-12715

Clinical and epidemiological features and impact of life habits in canine atopic dermatitis in Fortaleza, Brazil
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Ferreira, Tiago C.

; Nunes-Pinheiro, Diana Célia S.

; Cunha, Marina Gabriela M.C. Mori da

Pesquisa Veterinária Brasileira

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2023, Volume 43 elocation e07302

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT: Canine atopic dermatitis (cAD) is a worldwide allergic skin disease. The affected dog population can show different clinical patterns according to geographic region, and a lack of studies in Brazil is observed. Therefore, the aim of the present study was to assess the clinical and epidemiological data of cAD in dogs treated in a private clinical practice in Fortaleza, a city located in the Northeast Region of Brazil. cAD was diagnosed in 35% of dogs, being Shih-tzu and Poodle the most affected breeds. Paws and ears were frequently injured sites. Almost 50% of atopic dogs were diagnosed with superficial pyoderma and 36% with cutaneous malasseziosis. Atopic dogs with outdoor habits were less likely to develop cutaneous malassezial infection, and with routine ear, cleaning habits were less likely to develop bacterial otitis externa. In conclusion, canine atopic dermatitis is a prevalent disease in private clinical practice in Fortaleza, and lifestyle habits can be considered a risk factor for cutaneous malasseziosis infection and bacterial otitis externa in atopic dogs.

RESUMO: A dermatite atópica canina (DAC) é uma doença alérgica cutânea de ocorrência mundial. A população canina acometida pode apresentar diferentes padrões clínicos de acordo com a região geográfica e observa-se uma carência de estudos no Brasil. Portanto, o objetivo do presente estudo é avaliar os dados clínicos e epidemiológicos da DAC em cães atendidos em uma clínica privada em Fortaleza, cidade localizada na Região Nordeste do Brasil. A DAC foi diagnosticada em 35% dos cães, sendo Shih-tzu e Poodle as raças mais acometidas. As patas e as orelhas foram locais frequentemente afetados. Quase 50% dos cães atópicos foram diagnosticados com piodermite superficial e 36% com malasseziose cutânea. Cães atópicos com hábitos ao ar livre foram menos propensos a desenvolver malasseziose cutânea e com hábitos rotineiros de limpeza auricular foram menos propensos a desenvolver otite externa bacteriana. Em conclusão, a dermatite atópica canina é uma doença prevalente na prática clínica privada em Fortaleza e os hábitos de vida podem ser considerados como um fator de risco para infecção por malasseziose cutânea e otite externa bacteriana em cães atópicos.

https://doi.org/10.1590/1678-5150-pvb-7302

Genetic diversity and population structure of two Euglossini bee species in a host-parasite relationship
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MARTINS, DENILSON C.

; SANTOS JÚNIOR, JOSÉ E.

; FERREIRA, DHIEGO G.

; SOFIA, SILVIA H.

; ALBUQUERQUE, PATRÍCIA M.C.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 N. 3 elocation e20220579

Abstract: En | Text: En | PDF: En

Abstract In the current study, two euglossine species, Exaerete smaragdina and Eulaema nigrita, a cleptoparasite bee and its host, respectively, were used as models to: (i) access the genetic diversity and population structure of both species, sampled along a wide latitudinal range of Atlantic Forest, where the distribution of El. nigrita and Ex. smaragdina co-occurs; (ii) investigate the evolutionary history of these species through the Atlantic Forest, and in a wider scenario, to examine the evolutionary history of these species across others forest domains. Analyses involved males of El. nigrita and Ex. smaragdina sampled through Brazilian territory, including 19 sites in the Atlantic Forest. Bayesian Skyline Plot (BSP) was used to infer possible climate oscillations on population of both species over time. The BSP revealed stability in effective population size for both species in most of the Plio-Pleistocene period. However, BSP results aligned to the starlike configuration in the haplotype network, neutrality test, and population diversity patterns indicated population expansion of the two species during the late Pleistocene. Our findings suggest areas of potential refugia to the climatic oscillations of the Pleistocene in the Atlantic Forest in the Brazilian states of Espírito Santo for El. nigrita and Pernambuco for Ex. smaragdina.

https://doi.org/10.1590/0001-3765202320220579

Antifungal activity of a novel 3-Alkylpyridine analog derived from Marine sponge alkaloids
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ANDRADE, JÉSSICA T.

; LIMA, WILLIAM G.

; BARBOSA, CAMILA S.

; GONÇALVES, ALESSANDRA M.M.N.

; SILVA, MAYRA K.P.

; MORAIS, FERNANDA B.

; PALUMBO, JULIANA M.C.

; VIANA, GUSTAVO H.R.

; FERREIRA, JAQUELINE M.S.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 elocation e20200944

Abstract: En | Text: En | PDF: En

Abstract Candida spp. is considered an important cause of healthcare-associated infections worldwide. Currently, the emergence and spread of resistant Candida isolates are being increasingly reported, making the development of new agents urgently needed. In this study, we showed the in vitro anti-Candida activity of seven synthetic 3-alkylpyridine alkaloid analogs. Among them, alkaloid 1 presented a potent antifungal effect, which was independent of its capacity of binding with the fungal membrane ergosterol or cell wall. Analog 1 showed fungistatic and fungicidal effects against C. albicans (MIC 7.8 μg/mL and MFC 62.5 μg/mL), C. glabrata, C. krusei (MIC and MFC 31.2 μg/mL), and C. tropicalis (MIC 31.2 μg/mL and MFC 125 μg/mL). The time kill-curve study showed that compound 1 has a potent fungicidal effect in vitro, eliminating C. albicans cells. Furthermore, an in vitro synergistic effect with ketoconazole was observed for compound 1. This compound also eliminated the yeast-to-hypha transition. However, it showed high cytotoxicity against mammalian cells. Taken together, these findings support the use of compound 1 as a prototype to develop new anti-Candida agents, but molecular modifications should be done to minimize the high cytotoxicity obtained.

https://doi.org/10.1590/0001-3765202120200944 1 downloads

Efficacy of Imazamox with Centrifugal Energy Spray Nozzle on Eichhornia crassipes and Economic Analysis of Control Viability
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GARLICH, N.

; GUARNIERI, C.C.O.

; FREITAS, R.L.G.

; CERVONI, J.H.C.

; CRUZ, C.

; FERREIRA, M.C.

Planta Daninha

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2019, Volume 37 elocation e019194898

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Neste estudo foi avaliada a eficácia do imazamox para controle de E. crassipes, com bico de pulverização de energia centrífuga em doses e volumes de calda de pulverização e comparação do custo de controle mecânico e químico de plantas aquáticas. Foram utilizadas as doses de 200, 400 e 600 g i.a. ha-1 de imazamox e volumes de calda de 25, 50 e 100 L ha-1, além de um controle (sem aplicação do herbicida), com dez repetições. As aplicações foram feitas com bico de pulverização de energia centrífuga. As avaliações foram realizadas por porcentagem de controle em 7, 15, 21 e 30 dias após a aplicação (DAA), e a massa seca das plantas foi mensurada aos 30 DAA, assim como a retenção foliar das caldas de pulverização. Aos 30 DAA, em 200, 400 e 600 g i.a. ha-1 e 50 L ha-1, ocorreu eficácia de 96% a 100% e redução da massa seca das plantas de 88% a 100%. As doses e volumes de calda do imazamox foram eficazes no controle de E. crassipes, exceto 200 g i.a. ha-1 e 25 L ha-1. A maior retenção foliar do aguapé foi de 0,010 mL cm² na concentração de 400 g i.a. ha-1 e 50 L ha-1. O custo do controle químico foi 20 vezes menor que o mecânico, o que o torna economicamente mais viável que este. Esses resultados são importantes para a tomada de decisão sobre qual método e tecnologia de aplicação devem ser utilizados no controle de E. crassipes.

ABSTRACT: The efficacy of imazamox to control Eichhornia crassipes with a centrifugal energy spray nozzle at doses and volumes of spray solution and the costs of mechanical and chemical control of aquatic plants were evaluated in this study. Imazamox doses consisted of 200, 400, and 600 g a.i. ha-1, and spray solution volumes consisted of 25, 50, and 100 L ha-1, with a control (without herbicide application) and ten replications. Applications were performed with a centrifugal energy spray nozzle. The evaluations were carried out considering the percentage of control at 7, 15, 21, and 30 days after application (DAA), and plant dry matter was measured at 30 DAA, as well as leaf retention of spray solutions. An efficacy from 96 to 100% and plant dry matter reduction from 88 to 100% was observed at 30 DAA using imazamox doses of 200, 400, and 600 g a.i. ha-1 and a spray solution volume of 50 L ha-1. Imazamox doses and spray solution volumes were effective in controlling E. crassipes, except for 200 g a.i. ha-1 and 25 L ha-1, respectively. The highest leaf retention of E. crassipes was 0.010 mL cm2 at an imazamox dose of 400 g a.i. ha-1 and volume of 50 L ha-1. The cost of chemical control was 20 times lower when compared to mechanical control, making it economically more viable. These results are important for decision-making on which method and application technology should be used to control E. crassipes.

https://doi.org/10.1590/s0100-83582019370100102 312 downloads

Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology
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Baptista, M.S.

; Alves, M.J.M.

; Arantes, G.M.

; Armelin, H.A.

; Augusto, O.

; Baldini, R.L.

; Basseres, D.S.

; Bechara, E.J.H.

; Bruni-Cardoso, A.

; Chaimovich, H.

; Colepicolo Neto, P.

; Colli, W.

; Cuccovia, I.M.

; Da-Silva, A.M.

; Di Mascio, P.

; Farah, S.C.

; Ferreira, C.

; Forti, F.L.

; Giordano, R.J.

; Gomes, S.L.

; Gueiros Filho, F.J.

; Hoch, N.C.

; Hotta, C.T.

; Labriola, L.

; Lameu, C.

; Machini, M.T.

; Malnic, B.

; Marana, S.R.

; Medeiros, M.H.G.

; Meotti, F.C.

; Miyamoto, S.

; Oliveira, C.C.

; Souza-Pinto, N.C.

; Reis, E.M.

; Ronsein, G.E.

; Salinas, R.K.

; Schechtman, D.

; Schreier, S.

; Setubal, J.C.

; Sogayar, M.C.

; Souza, G.M.

; Terra, W.R.

; Truzzi, D.R.

; Ulrich, H.

; Verjovski-Almeida, S.

; Winck, F.V.

; Zingales, B.

; Kowaltowski, A.J.

Brazilian Journal of Medical and Biological Research

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2019, Volume 52 N. 9 elocation e8935

Abstract: En | Text: En | PDF: En

The scientific publication landscape is changing quickly, with an enormous increase in options and models. Articles can be published in a complex variety of journals that differ in their presentation format (online-only or in-print), editorial organizations that maintain them (commercial and/or society-based), editorial handling (academic or professional editors), editorial board composition (academic or professional), payment options to cover editorial costs (open access or pay-to-read), indexation, visibility, branding, and other aspects. Additionally, online submissions of non-revised versions of manuscripts prior to seeking publication in a peer-reviewed journal (a practice known as pre-printing) are a growing trend in biological sciences. In this changing landscape, researchers in biochemistry and molecular biology must re-think their priorities in terms of scientific output dissemination. The evaluation processes and institutional funding for scientific publications should also be revised accordingly. This article presents the results of discussions within the Department of Biochemistry, University of São Paulo, on this subject.

https://doi.org/10.1590/1414-431x20198935 1769 downloads

Does the Use of Adjuvants Alter Surface Tension and Contact Angle of Herbicide Spray Droplets on Leaves of Sida spp.?
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SANTOS, R.T.S.

; FERREIRA, M.C.

; VIANA, R.G.

Planta Daninha

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2019, Volume 37 elocation e019185603

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O espalhamento das gotas sobre superfícies vegetais pode indicar melhor eficiência numa aplicação de herbicidas. Com este trabalho, objetivou-se avaliar a tensão superficial e o ângulo de contato de gotas do herbicida aminopiralide + fluroxipir associado a adjuvantes sobre a superfície foliar de três espécies do gênero Sida. O experimento foi realizado em delineamento inteiramente ao acaso, disposto em arranjo fatorial 4x2+1, com quatro repetições. Foram utilizadas oito caldas contendo duas dosagens do herbicida (0,04 + 0,08 kg i.a. ha-1 e 0,08+0,16 kg i.a. ha -1), associado ou não aos adjuvantes óleo vegetal, óleo mineral e lecitina, e água como testemunha. Foram feitas medições da tensão superficial e de ângulo de contato das caldas com tensiômetro. A tensão superficial foi quantificada aos 5, 15 e 25 segundos após a formação da gota. O ângulo de contato foi medido nos tempos de 5, 15 e 25 segundos, após depósito das gotas sobre as superfícies adaxial e abaxial de folhas de Sida rhombifolia, S. glaziovii e S. cordifolia. Os resultados foram submetidos à análise de variância, pelo teste F, e as médias dos tratamentos, comparadas pelo teste de Tukey (p>0,05). Não houve interação entre os fatores para tensão superficial. Os ângulos de contato de S. cordifolia e S. glaziovii foram menores após a adição da lecitina na face adaxial. A adição dos adjuvantes na calda proporcionou menores ângulos de contato na dosagem de 0,04 + 0,08 kg i.a. ha-1, exceto para S. rhombifolia, cujo ângulo de contato foi menor com a calda sem adjuvante.

ABSTRACT: Droplet spreading on plant surfaces may indicate greater efficiency in herbicide application. The aim of this study was to evaluate surface tension and contact angle of the aminopyralid + fluroxypir herbicide droplets associated with adjuvants on the leaf surface of three species of the genus Sida. The experiment was carried out in a completely randomized design, in a 4x2+1 factorial arrangement, with four replications. Four treatments containing two rates of the herbicide (0.04 + 0.08 kg a.i. ha-1 and 0.08+0.16 kg a.i. ha-1) were evaluated, associated or not with the adjuvants vegetable oil, mineral oil and lecthin; and water was used as a control. Surface tension and contact angle of the syringes were measured with a tensiometer. Surface tension was evaluated at 5, 15 and 25 seconds after droplet formation. Contact angle was measured at 5, 15 and 25 seconds after droplet deposition on the adaxial and abaxial surfaces of Sida rhombifolia, S. glaziovii and S. cordifolia. The results were submitted to analysis of variance by the F-test and the means of the treatments were compared by Tukey’s test (p>0.05). There was no interaction between the factors for surface tension. The contact angles of S. cordifolia and S. glaziovii were lower after addition of lecthin on the adaxial face. The addition of the adjuvants to the spray solution provided lower contact angles at the rate of 0.04 + 0.08 kg a.i. ha-1, except for S. rhombifolia, whose contact angle was lower with the spray solution without adjuvant.

https://doi.org/10.1590/s0100-83582019370100082 524 downloads

Effect of Pesticide Addition Sequence on the Preparation of Phytosanitary Spray Solutions
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RAMOS, M.F.T.

; SANTOS, R.T.S.

; ALMEIDA, D.P.

; VECHIA, J.F.D.

; FERREIRA, M.C.

Planta Daninha

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2019, Volume 37 elocation e019185517

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A adição de adjuvantes às caldas herbicidas visa preservar ou aumentar o efeito biológico do tratamento, porém é comumente realizada sem conhecimento das interações físico-químicas entre os produtos. Assim, objetivou-se neste trabalho avaliar os efeitos sobre a tensão superficial e o ângulo de contato decorrentes de diferentes sequências de adição do herbicida aminopiralide + fluroxipir e adjuvantes no preparo de caldas fitossanitárias. Foram realizados dois experimentos, com a dosagem de 1 e 2 L ha-1 do herbicida, associados aos adjuvantes óleo mineral (OM), copolímero de poliéter e silicone (SIL), mistura de fosfatidicolina e ácido propiônico (LEC), todos na proporção de 0,3% v v-1. A taxa de aplicação considerada foi de 150 L ha-1. Mediu-se a tensão superficial pelo método da gota pendente. O ângulo de contato foi medido nas superfícies adaxial e abaxial de folhas da planta daninha de pastagens Senna obtusifolia e em parafilme. Verificou-se que a ordem de preparo não alterou o ângulo de contato em nenhuma das superfícies analisadas na dosagem de 1 L ha-1 do herbicida. Para a dosagem de 2 L ha-1, os adjuvantes SIL e LEC apresentaram maior espalhamento quando adicionados previamente ao herbicida. O OM resultou em maior espalhamento quando adicionado após o herbicida, com maior cobertura da superfície. Portanto, a sequência de preparo das caldas fitossanitárias influencia no espalhamento destas sobre os alvos.

ABSTRACT: The addition of adjuvants to herbicide solutions is aimed at preserving or enhancing the biological effect of treatment. However, it is commonly performed without knowledge of the physicochemical interactions between products. This study aimed to assess the effects of different addition sequences of the herbicide aminopyralid + fluroxypyr and adjuvants in the preparation of phytosanitary spray solutions on the surface tension and contact angle. Two experiments were carried out with herbicide doses of 1 and 2 L ha-1 associated with the adjuvants mineral oil (MO), silicone-polyether copolymer (SIL), and a mixture of phosphatidylcholine (lectin) and propionic acid (LEC), all at a proportion of 0.3% v v-1. The application rate was 150 L ha-1. Surface tension was measured by the pendant droplet method. Contact angle was measured on the adaxial and abaxial surfaces of leaves of the pasture weed Senna obtusifolia and parafilm. Preparation sequence did not change the contact angle on any of the analyzed surfaces at a dose of 1 L ha-1 of herbicide. For the dose of 2 L ha-1, the adjuvants SIL and LEC showed a higher spreading when previously added to the herbicide. MO resulted in a higher spreading when added after the herbicide, with higher surface coverage. Therefore, the preparation sequence influences the dispersion of phytosanitary spray solutions on targets.

https://doi.org/10.1590/s0100-83582019370100075 620 downloads

Sepsis: evolving concepts and challenges
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Salomão, R.

; Ferreira, B.L.

; Salomão, M.C.

; Santos, S.S.

; Azevedo, L.C.P.

; Brunialti, M.K.C.

Brazilian Journal of Medical and Biological Research

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2019, Volume 52 N. 4 elocation e8595

Abstract: En | Text: En | PDF: En

Sepsis remains a major cause of morbidity and mortality worldwide, with increased burden in low- and middle-resource settings. The role of the inflammatory response in the pathogenesis of the syndrome has supported the modern concept of sepsis. Nevertheless, a definition of sepsis and the criteria for its recognition is a continuous process, which reflects the growing knowledge of its mechanisms and the success and failure of diagnostic and therapeutic interventions. Here we review the evolving concepts of sepsis, from the “systemic inflammatory response syndrome triggered by infection” (Sepsis-1) to “a severe, potentially fatal, organic dysfunction caused by an inadequate or dysregulated host response to infection” (Sepsis-3). We focused in the pathophysiology behind the concept and the criteria for recognition and diagnosis of sepsis. A major challenge in evaluating the host response in sepsis is to characterize what is protective and what is harmful, and we discuss that, at least in part, the apparent dysregulated host response may be an effort to adapt to a hostile environment. The new criteria for recognition and diagnosis of sepsis were derived from robust databases, restricted, however, to developed countries. Since then, the criteria have been supported in different clinical settings and in different economic and epidemiological contexts, but still raise discussion regarding their use for the identification versus the prognostication of the septic patient. Clinicians should not be restricted to definition criteria when evaluating patients with infection and should wisely use the broad array of information obtained by rigorous clinical observation.

https://doi.org/10.1590/1414-431x20198595 9196 downloads

10.

Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly
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Mendes, G.A.

; Haag, T.

; Trott, G.

; Rech, C.G.S.L.

; Ferreira, N.P.

; Oliveira, M.C.

; Kohek, M.B.

; Pereira-Lima, J.F.S.

Brazilian Journal of Medical and Biological Research

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2018, Volume 51 N. 2 elocation e6808

Abstract: En | Text: En | PDF: En

Pituitary adenomas account for 10–15% of primary intracranial tumors. Growth hormone (GH)-secreting adenomas account for 13% of all pituitary adenomas and cause acromegaly. These tumors can be aggressive, invade surrounding structures and are highly recurrent. The objective of this study was to evaluate E-cadherin, Slug and neural cell adhesion molecule (NCAM) expression in GH-secreting pituitary adenomas and its relationship to tumor invasiveness. A cross–sectional study of patients who underwent hypophysectomy due to GH-secreting pituitary adenoma from April 2007 to December 2014 was carried out. The medical records were reviewed to collect clinical data. Immediately after surgery, tumor samples were frozen in liquid nitrogen and stored in a biofreezer at –80°C for assessment of E-cadherin 1 (CDH1), SLUG (SNAI2), and NCAM (NCAM1) by real-time PCR. The samples were fixed in formalin and embedded in paraffin for immunohistochemical analysis of E-cadherin and NCAM. Thirty-five patients with acromegaly were included in the study. Of these, 65.7% had invasive tumors. Immunohistochemically, E-cadherin was expressed in 96.7% of patients, and NCAM in 80% of patients. There was no statistically significant relationship between tumor grade or invasiveness and immunohistochemical expression of these markers. Regarding gene expression, 50% of cases expressed CDH1, none expressed SNAI2, and 53.3% expressed NCAM1. There was no statistically significant relationship between tumor grade or invasiveness and gene expression of CDH1, SNAI2, and NCAM1. The absence of Slug overexpression and of E-cadherin and NCAM suppression suggests that expression of these markers is not associated with tumor invasiveness in GH-secreting pituitary adenomas.

https://doi.org/10.1590/1414-431x20176808 1523 downloads

11.

Floating Macrophyte Control with Glyphosate According to Adjuvants and Spray Nozzles
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SILVA, A.F.

; CERVEIRA JR, W.R.

; FERREIRA, M.C.

; CRUZ, C.

Planta Daninha

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2018, Volume 36 elocation e018168125

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo deste estudo foi avaliar a aplicação do herbicida glyphosate acrescido de três adjuvantes e três pontas de pulverização no controle da macrófita Pistia stratiotes. Para isso, após a colonização de 80 a 90%, foi realizada a aplicação de 1,5, 3,5 e 7,0 L ha-1 de glyphosate isolado e com os adjuvantes Aterbane® BR, Veget’oil® e Dash® HC (0,5% vv-1) e pontas de pulverização (AI 110.02, TTI 110.015 e TT 110.015) e um controle (água). As aplicações foram feitas com pulverizador costal pressurizado de precisão, à pressão constante de 50 p.s.i. e consumo de calda de 200 L ha-1. Na dose de 1,5 L ha-1 houve 100% de controle para a combinação glyphosate + Veget’oil + a ponta AI 110.02 (Gly+V.oil+AI), Gly+Aterb.+TTI e Gly+Dash+TT em 15 dias após aplicação (DAA). Em 3,5 L ha-1 o maior controle (95%) foi para Gly+AI, Gly+Aterb.+TTI, Gly+V.oil+TTI, Gly+Dash+TTI, Gly+Aterb.+TT e Gly+Dash+TT, e em 7,0 L ha-1 chegou-se a 100% para Gly+AI, Gly+Aterb.+AI, Gly+V.oil+AI, Gly+Aterb.+TTI, Gly+V.oil+TTI, Gly+Dash+TTI, Gly+TT e Gly+Dash+TT. Assim, conclui-se que em 1,5 L ha-1 a combinação de glyphosate com o Dash® e a ponta TT 110.015 (Gly+Dash+TT) foi a mais promissora; em 3,5 L ha-1 foi Gly+Aterb.+TTI e em 7,0 L ha-1 as pontas com indução de ar (AI 110.02 e TTI 110.015), independentemente de o glyphosate estar isolado ou com qualquer um dos três adjuvantes, foram as melhores no estabelecimento do controle.

ABSTRACT: The goals of this study were to evaluate the application of glyphosate plus three adjuvants and three spray nozzles in controlling the macrophyte Pistia stratiotes. For this purpose, after an 80 to 90% colonization, the application of 1.5, 3.5 and 7.0 L h-1 of isolated glyphosate and with the adjuvants Aterbane® BR, Veget’oil® and Dash® HC (0.5% vv-1) and the spray nozzles (AI 110.02, TTI 110.015 and TT 110.015) was performed, plus a control sample (water). Applications were performed with a pressurized backpack precision sprayer, at the constant pressure of 50 p.s.i. and with mixture consumption of 200 L ha-1. At the dose of 1.5 L ha-1, there was 100% control for the combination glyphosate + Veget’oil + tip AI 110.02 (Gly+V.oil+AI, Gly+Aterb.+TTI and Gly+Dash+TT) 15 days after application (DAA). At 3.5 L ha-1, the greatest control (95%) was with Gly+AI, Gly+Aterb.+TTI, Gly+V.oil+TTI, Gly+Dash+TTI, Gly+Aterb.+TT and Gly+Dash+TT; and at 7.0 L ha-1 100% was observed for Gly+AI, Gly+Aterb.+AI, Gly+V.oil+AI, Gly+Aterb.+TTI, Gly+V.oil+TTI, Gly+Dash+TTI, Gly+TT and Gly+Dash+TT. Thus, it is possible to conclude that at 1.5 L ha-1, the combination of glyphosate plus Dash® and with the nozzle TT 110.015 (Gly+Dash+TT) was the most promising one; at 3.5 L ha-1, it was Gly+Aterb.+TTI and at 7.0 L ha-1 the air induction nozzles (AI 110.02 and TTI 110.015), whether with isolated glyphosate or with any of the three adjuvants, were the best at providing control.

https://doi.org/10.1590/s0100-83582018360100027 1335 downloads

12.

Imunogenicidade da proteína M recombinante de Streptococcus equi subsp. equi coadministrada com um adjuvante molecular
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Maciel, L.F.

; Magalhães, C.G.

; Moraes, C.M.

; Rosa, M.C.

; Mendonça, M.

; Moreira, A.N.

; Dummer, L.A.

; Finger, P.F.

; Ferreira, M.R.A.

; Leite, F.P.L.

; Conceição, F.R.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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oct 2017, Volume 69 N. 5 Pages 1351 - 1356

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

ABSTRACT The strangles is an infectious disease that affects horses from all ages and causes important economic losses in the equine-related business. The aim of this work was to evaluate the immunogenicity of the recombinant M protein from Streptococcus equi (rSeM) co-administered with the recombinant heat-labile enterotoxin B subunit from Escherichia coli (rLTB) in mice and horses. A total of 72 female Balb-c mice were divided into eight groups and 18 horses were divided into six groups. The animals were inoculated by intramuscular (IM) or intranasal (IN) routes with different treatments of rSeM, rLTB and/or Al(OH)3. The results obtained in both species, independent of administration routes, demonstrated that rSeM + rLTB had higher levels of specific serum immunoglobulins, however, in mucosal immunity the increase was not identified. Thus, the use of rSeM as vaccine antigen and rLTB as adjuvant can be a potential tool in the control of equine strangles.

https://doi.org/10.1590/1678-4162-9288 1284 downloads

13.

Application Volumes and Sizes of Droplets for the Application of Diquat Herbicide in the Control of Eichhornia crassipes
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ALMEIDA, D.P.

; AGOSTINI, A.R.

; YAMAUCHI, A.K.

; DECARO JR., S.T.

; FERREIRA, M.C.

Planta Daninha

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mar 2016, Volume 34 N. 1 Pages 171 - 179

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivou-se neste estudo avaliar a eficiência da aplicação e eficácia de controle de aguapé (Eichhornia crassipes) com o uso do herbicida diquat veiculado com dois volumes de aplicação. associado a três classes de gotas. Foram conduzidos três experimentos: no primeiro avaliou-se a eficiência da aplicação; no segundo, a eficácia de controle; e, no terceiro, o espectro de gotas, conduzidos em delineamento inteiramente casualizado, com cinco, quatro e seis repetições, respectivamente. Os tratamentos foram dispostos em esquema fatorial 2 x 3, com dois volumes de aplicação (100 e 200 L ha-1) e três classes de gotas (fina, extremamente grossa e ultragrossa) para o primeiro e o segundo experimento, e o terceiro foi constituído por duas concentrações do herbicida diquat na calda (2 e 4 g de i.a. L-1) e por três classes de gotas (fina, extremamente grossa e ultragrossa). A eficiência da aplicação foi determinada pela cobertura por gotas, deposição de calda e ingrediente ativo do herbicida (diquat). Já a eficácia foi quantificada pelo controle e pela porcentagem de plantas com rebrote até 50 dias após a aplicação. Foi analisado o espectro das gotas produzidas por cada modelo de pontas utilizado para obter as classes de gotas. De acordo com os parâmetros analisados, o uso das classes de gotas extremamente grossa e ultragrossa pode proporcionar maior segurança na aplicação do herbicida diquat em ambiente aquático, associado à deposição do ativo e cobertura suficiente para controle de E. crassipes com ambos os volumes de aplicação.

The objective of this study was to evaluate the efficiency of application and the efficacy of control of water hyacinth (Eichhornia crassipes) with the use of the diquat herbicide done with two application volumes associated to three droplets classes. Three experiments were conducted; in the first, the application efficiency was evaluated; in the second, the control efficiency and; in the third, the droplet spectrum. They were conducted in a completely randomized design with five, four and six replicates, respectively. The treatments were arranged in a 2 x 3 factorial design, with two application volumes (100 and 200 L ha-1) and three droplets classes (fine, extremely coarse and ultra-coarse) to the first and second experiment and the third comprised two diquat concentrations in spray solution (2 and 4 g i.a. L-1) and three droplets classes (fine, extremely coarse and ultra-coarse). The application efficiency was determined by the coverage by droplets, spray deposition and active ingredient of the herbicide (diquat). The efficacy was measured by the control and the percentage of plants with regrowth at 50 days after application. The spectrum of droplets produced per each nozzle model used to obtain the droplets classes were analyzed. According to the parameters analyzed, using the droplets classes extremely thick and ultra thick can provide greater certainty in the application of diquat in the aquatic environment associated with the deposition of the active and sufficient coverage to control Eichhornia crassipes with both application volumes

https://doi.org/10.1590/S0100-83582016340100018 1542 downloads

14.

Expression of merlin, NDRG2, ERBB2, and c-MYC in meningiomas: relationship with tumor grade and recurrence
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Ongaratti, B.R.

; Silva, C.B.O.

; Trott, G.

; Haag, T.

; Leães, C.G.S.

; Ferreira, N.P.

; Oliveira, M.C.

; Pereira-Lima, J.F.S.

Brazilian Journal of Medical and Biological Research

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2016, Volume 49 N. 4 elocation e5125

Abstract: En | Text: En | PDF: En

Meningiomas are common, usually benign tumors of the central nervous system that have a high rate of post-surgical recurrence or regrowth. We determined expression of the proteins merlin, NDRG2, ERBB2, and c-MYC in meningiomas using immunohistochemistry and assessed relationships between protein expression and gender, age, tumor grade, and recurrence or regrowth. The study sample comprised 60 patients, (44 women and 16 men) with a mean age of 53.2±12.7 years. Tumors were classified as grade I (n=48) or grades II and III (n=12). Expression of merlin, NDRG2, ERBB2, and c-MYC was not significantly different statistically with relation to gender, age, or meningioma recurrence or regrowth. Merlin was expressed in 100% of the cases. No statistically significant difference between tumor grade and recurrence or regrowth was identified. Statistically significant differences were identified between the mean age of patients with grade I (54.83±11.60) and grades II and III (46.58±15.08) meningiomas (P=0.043), between strong c-MYC expression and grades II and III (P<0.001), and between partial surgical resection and tumor recurrence or regrowth (P<0.001). These findings reveal the lower mean age among grades II and III meningioma patients than grade I patients, the influence of the protein merlin on tumorigenesis, the association of c-MYC with aggressive meningiomas, and that partial surgical resection is associated with tumor recurrence or regrowth.

https://doi.org/10.1590/1414-431X20155125 1371 downloads

15.

Coral-based climate records from tropical South Atlantic: 2009/2010 ENSO event in C and O isotopes from Porites corals (Rocas Atoll, Brazil)
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Pereira, Natan S.

; Sial, Alcídes N.

; Kikuchi, Ruy K.P.

; Ferreira, Valderez P.

; Ullmann, Clemens V.

; Frei, Robert

; Cunha, Adriana M.C.

Anais da Academia Brasileira de Ciências

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dec 2015, Volume 87 N. 4 Pages 1939 - 1957

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Esqueletos de corais contêm excelentes registros de condições ambientais passada, devido à sua longevidade e assinaturas geoquímicas bem calibradas. Registros de isótopos estáveis de C e O de isótopos em corais são especialmente interessantes, porque eles podem destacar a variabilidade multidecadal das condições climáticas locais além do registro instrumental com alta fidelidade e resolução sub-anual. Aqui nós relatamos um dos primeiros registros climáticos com base em coral do Atlântico Sul Equatorial, através de perfis isotópicos de duas colônias de Porites sp. do Atol das Rocas, um localchave para reconstruções climáticas e anteriormente não estudada. Aqui são apresentados dados de perfis ao longo do vale entre coralitos de uma colónia e na parte central do leque de coralitos de outra colónia. Embora exista diferenças significativas nos valores isotópicos entre as duas colônias, ainda são observados em ambas o registro do evento do ENSO 2009/2010 - um episódio de branqueamento do coral mundial - através de valores anômalos de δ18O negativos (até 1 permil mais negativo do que os valores de base). Valores de isótopos estáveis de C também aparentam ser afetada pelo evento do ENSO. Nossos resultados indicam que os corais do Atol das Rocas são substratos ideais para monitorar grandes oscilações climáticas no Oceano Atlântico tropical Sul.

ABSTRACT Coral skeletons contain records of past environmental conditions due to their long life span and well calibrated geochemical signatures. C and O isotope records of corals are especially interesting, because they can highlight multidecadal variability of local climate conditions beyond the instrumental record, with high fidelity and sub-annual resolution. Although, in order to get an optimal geochemical signal in coral skeleton, sampling strategies must be followed. Here we report one of the first coral-based isotopic record from the Equatorial South Atlantic from two colonies of Porites astreoides from the Rocas Atoll (offshore Brazil), a new location for climate reconstruction. We present time series of isotopic variation from profiles along the corallite valley of one colony and the apex of the corallite fan of the other colony. Significant differences in the isotopic values between the two colonies are observed, yet both record the 2009/2010 El Niño event - a period of widespread coral bleaching - as anomalously negative δ18O values (up to −1 permil). δ13C is found to be measurably affected by the El Niño event in one colony, by more positive values (+0.39 ‰), and together with a bloom of endolithic algae, may indicate physiological alteration of this colony. Our findings indicate that corals from the Rocas Atoll can be used for monitoring climate oscillations in the tropical South Atlantic Ocean.

https://doi.org/10.1590/0001-3765201520150072 2688 downloads

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