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ABSTRACT: In Brazil, urea is the most used nitrogen (N) fertilizer to improve forage production. However, their excessive use can cause environmental impacts through N losses, such as ammonia (NH3) volatilization. Therefore, the current study adjusted and estimated the NH3 volatilization from urea applied on tropical pastures in three rainfall conditions using mathematical models. Data were collected from Marandu grass (Brachiaria brizantha) fertilized with 50 kg N ha-1 during wet, intermediate, and dry conditions. Ammonia volatilization was measured in five semi-open chambers for 21 days. The linear, quadratic, exponential, Gompertz, Groot, and Richards models were tested for fitting and estimating the NH3 volatilization. The Gompertz, Groot, and Richards models generated predictions similar to the observed data, with a high determination coefficient, indicating a better fit of these equations to data, with precision and accuracy. However, the Groot model was selected due to the lowest root mean square error of prediction (0.29 % total N lost as NH3). The greatest N loss as NH3 volatilization occurred in the wet, followed by intermediate and dry conditions (20.2, 17.0, and 11.3 % total N lost as NH3, respectively). Therefore, nitrogen losses as NH3 volatilization after application of 50 kg N ha-1, as urea source, are altered according to the weather conditions, reaching 20% of N added in the wet rainfall period. The Groot model is recommended for fitting and estimating the NH3 volatilization from urea applied on Marandu grass pastures in the wet and dry rainfall conditions.

RESUMO: No Brasil, a ureia é o fertilizante nitrogenado mais utilizado para melhorar a produção de forragem. No entanto, seu uso excessivo pode causar impactos ambientais por meio de perdas de nitrogênio (N), como a volatilização da amônia (NH3). Portanto, o objetivo do presente estudo foi ajustar a volatilização de NH3 da ureia aplicada em pastos tropicais em três condições de chuva utilizando modelos matemáticos. Dados foram coletados de pastos de capim-marandu (Brachiaria brizantha) adubado com 50 kg N ha-1 em condições úmidas, intermediárias e secas. A volatilização da NH3 foi medida em cinco câmaras semiabertas durante 21 dias. Os modelos, linear, quadrático, exponencial, Gompertz, Groot e Richards foram testados para ajuste e estimativa da volatilização do NH3. Os modelos de Gompertz, Groot e Richards geraram predições semelhantes aos dados observados, com alto coeficiente de determinação, indicando um melhor ajuste dessas equações aos dados, com acurácia e precisão. No entanto, o modelo Groot foi selecionado devido ao menor erro quadrático médio das predições (0,29% de N total perdido como NH3). A maior volatilização de NH3 ocorreu em condições climáticas úmida, seguido por intermediária e seca (20,2; 17,0 e 11,3% de N total perdido como NH3, respectivamente). Portanto, as perdas de N como volatilização de NH3 após a aplicação de 50 kg N ha-1, como fonte de ureia, são alteradas de acordo com as condições climáticas, atingindo a 20% do N adicionado nas condições úmidas. O modelo Groot é recomendado para ajuste e estimativa da volatilização de NH3 da ureia aplicada em pastos de capim Marandu em condições úmidas e secas.

https://doi.org/10.1590/0103-8478cr20230230

Caracterização do ambiente alimentar de uma universidade pública do estado do Rio de Janeiro
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Batista, Caroline de Araújo

; Pereira, Alessandra da Silva

; Dias, Juliana Furtado

; Santos, Claúdia Roberta Bocca

; Aquino, Luana Azevedo de

; Leão, Leila Sicupira Carneiro de Souza

; Franco, Amanda da Silva

Cadernos Saúde Coletiva

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2023, Volume 31 N. 1 elocation e31010492

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Background The food environment can influence food and beverage choice by the population and their nutritional status. Objective To characterize the food environment of campuses at the Federal University of Rio de Janeiro. Method A cross-sectional study of food and beverage establishments within the university was carried out. The assessment of the food environment was performed with the application of an instrument to collect information: type, food or preparations, mode, characteristics, menu and nutritional information, promotions, advertisements, availability of food or drinks, variety, and price. The observed foods and preparations, the presence of an approach such as nutritional information, advertisements, and promotions were classified as facilitators/barriers for healthy eating, according to the Food Guide for the Brazilian Population. Results Of the 5 establishments that participated, 4 were commercial and 1 was a university restaurant (RU). All establishments accepted cash and cards, and most had a menu and price. None had nutritional information. The supply of fruits and vegetables was found in 4 establishments. The availability of artificial sweetener was found in all locations. A wide variety of candies (8 ± 7.1), soft drinks (9 ± 3.7), chocolate (8 ± 7.1), salted biscuits (8 ± 12.3), and sweets (7 ± 9.1) were observed. Fruits had a higher average price, R$3.83, when compared to savory foods, R$ 2.85, and biscuits, R$ 1.50. Conclusion The university food environment presented a greater offer of unhealthy foods, presenting barriers to the promotion of more appropriate eating practices.

Resumo Introdução O ambiente alimentar pode influenciar as escolhas alimentares e de bebidas pela população e seu estado nutricional. Objetivo Caracterizar o ambiente alimentar dos campi de universidade federal do Rio de Janeiro. Método Estudo transversal com estabelecimentos que comercializavam alimentos e bebidas dentro dos campi da universidade. A avaliação do ambiente alimentar foi realizada com a aplicação de um instrumento para coleta das informações: tipo de estabelecimento, alimentos/preparações, modalidade, características, cardápio e informações nutricionais, promoções, propagandas, disponibilidade de alimentos/bebidas, variedade e preço. Os alimentos e preparações observados, a presença de estratégias como informação nutricional, propagandas e promoções foram classificados em facilitadores/ barreiras para alimentação saudável, de acordo com o Guia Alimentar para a População Brasileira. Resultados Participaram do estudo cinco estabelecimentos, quatro eram comerciais e um o restaurante universitário (RU). Todos os estabelecimentos aceitavam dinheiro e cartão, havia cardápio e preço na maioria. Nenhum apresentava informação nutricional. A oferta de frutas/hortaliças foi encontrada em quatro estabelecimentos. A disponibilidade de edulcorante artificial foi encontrada em todos os locais. Observou-se grande variedade de balas (8 ± 7,1), refrigerantes (9 ± 3,7), chocolate (8 ± 7,1), biscoito salgado (8 ± 12,3) e doce (7 ± 9,1). Frutas apresentaram preço médio superior, R$ 3,83, quando comparadas aos salgados, R$ 2,85 e biscoitos, R$ 1,50. Conclusão O ambiente alimentar universitário apresentou maior oferta de alimentos não saudáveis, apresentando barreiras na promoção de práticas alimentares mais adequadas.

https://doi.org/10.1590/1414-462x202331010492

Clinical characteristics and outcomes of patients with COVID-19 admitted to the intensive care unit during the first and second waves of the pandemic in Brazil: a single-center retrospective cohort study
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Corrêa, Thiago Domingos

; Midega, Thais Dias

; Cordioli, Ricardo Luiz

; Barbas, Carmen Silvia Valente

; Rabello Filho, Roberto

; Silva, Bruno Caldin da

; Silva Júnior, Moacyr

; Nawa, Ricardo Kenji

; Carvalho, Fabrício Rodrigues Torres de

; Matos, Gustavo Faissol Janot de

; Lucinio, Neide Marcela

; Rodrigues, Rodrigo Dias

; Eid, Raquel Afonso Caserta

; Bravim, Bruno de Arruda

; Pereira, Adriano José

; Santos, Bento Fortunato Cardoso dos

; Pinho, João Renato Rebello

; Pardini, Andreia

; Teich, Vanessa Damazio

; Laselva, Claudia Regina

; Cendoroglo Neto, Miguel

; Klajner, Sidney

; Ferraz, Leonardo José Rolim

einstein (São Paulo)

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2023, Volume 21 elocation eAO0233

Abstract: En | Text: En | PDF: En

ABSTRACT Objective To describe and compare the clinical characteristics and outcomes of patients admitted to intensive care units during the first and second waves of the COVID-19 pandemic. Methods In this retrospective single-center cohort study, data were retrieved from the Epimed Monitor System; all adult patients admitted to the intensive care unit between March 4, 2020, and October 1, 2021, were included in the study. We compared the clinical characteristics and outcomes of patients admitted to the intensive care unit of a quaternary private hospital in São Paulo, Brazil, during the first (May 1, 2020, to August 31, 2020) and second (March 1, 2021, to June 30, 2021) waves of the COVID-19 pandemic. Results In total, 1,427 patients with COVID-19 were admitted to the intensive care unit during the first (421 patients) and second (1,006 patients) waves. Compared with the first wave group [median (IQR)], the second wave group was younger [57 (46-70) versus 67 (52-80) years; p<0.001], had a lower SAPS 3 Score [45 (42-52) versus 49 (43-57); p<0.001], lower SOFA Score on intensive care unit admission [3 (1-6) versus 4 (2-6); p=0.018], lower Charlson Comorbidity Index [0 (0-1) versus 1 (0-2); p<0.001], and were less frequently frail (10.4% versus 18.1%; p<0.001). The second wave group used more noninvasive ventilation (81.3% versus 53.4%; p<0.001) and high-flow nasal cannula (63.2% versus 23.0%; p<0.001) during their intensive care unit stay. The intensive care unit (11.3% versus 10.5%; p=0.696) and in-hospital mortality (12.3% versus 12.1%; p=0.998) rates did not differ between both waves. Conclusion In the first and second waves, patients with severe COVID-19 exhibited similar mortality rates and need for invasive organ support, despite the second wave group being younger and less severely ill at the time of intensive care unit admission.

https://doi.org/10.31744/einstein_journal/2023ao0233

LED phototherapy in tissue repair of chronic wounds in people with diabetes: a systematic review
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Baracho, Valéria da Silva

; Silva, Natália Cristina da

; Peixoto, Marco Fabrício Dias

; Sampaio, Kinulpe Honorato

; Cordeiro, Cláudia Aparecida Fernandes

; Lucas, Thabata Coaglio

Revista Gaúcha de Enfermagem

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2023, Volume 44 elocation e20220274

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT Objective: To identify scientific evidence of LED photobiomodulation in the treatment and tissue repair of chronic wounds in people with Diabetes Mellitus, types I and II. Method: Systematic review conducted from September/2021 to April/2022 in PubMed, LILACS, SCIELO, COHRANE, EMBASE and Web of Science. Randomized and observational clinical trials using LED in wound healing in diabetics, published between 2015 and 2022 were included. Data were descriptively analyzed with title/abstract screening, full text articles reading and definitive selection after meeting the predefined inclusion and exclusion criteria. Results: Fromthe total of 840 references, eight articles were selected, that evaluated the effectiveness of LED phototherapy in wounds of diabetic patients. Conclusion: LED light proved to be beneficial in tissue repair, with increased production in collagen and fibroblasts, angiogenesis, reduction of inflammation and, consequently, a decrease in lesion size.

RESUMEN Objetivo: Identificar evidencias científicas de fotobiomodulación con LED en el tratamiento y reparación de tejidos de heridas crónicas en personas con Diabetes Mellitus, tipos I y II. Método: Revisión sistemática realizada de septiembre/2021 a abril/2022 en PubMed, LILACS, SCIELO, COHRANE, EMBASE y Web of Science. Se incluyeron ensayos clínicos aleatorizados y observacionales con uso de LED en la cicatrización de heridas en diabéticos, publicados entre 2015 y 2022. Los datos fueron analizados descriptivamente con selección de título/resumen, lectura de artículos a texto completo y selección definitiva después de cumplir con la inclusión e inclusiones ex - predefinidas. Resultados: Del total de 840 referencias encontradas, se seleccionaron ocho artículos que evaluaron la efectividad de la fototerapia LED en heridas de pacientes diabéticos. Conclusión: La luz LED demostró ser beneficiosa en la reparación de tejidos, con aumento de la producción de colágeno y fibroblastos, angiogénesis, reducción de la inflamación y, en consecuencia, disminución del tamaño de la lesión.

RESUMO Objetivo: Identificar evidências científicas da fotobiomodulação com LED no tratamento e reparo tecidual em feridas crônicas de pessoas com Diabetes Mellitus, tipo I e II. Método: Revisão sistemática realizada de setembro/2021 a abril/2022 na PubMed, LILACS, SCIELO, COHRANE, EMBASE e Web of Science. Incluídos ensaios clínicos randomizados e observacionais utilizando LED na cicatrização de feridas em diabéticos, publicados entre 2015 a 2022. Os dados foram analisados descritivamente com triagem de título/resumo, leitura dos artigos em texto completo e seleção definitiva após atender aos critérios de inclusão e exclusão pré-definidos. Resultados: Do total de 840 referências encontradas, foram selecionados oito artigos que avaliaram a eficácia da fototerapia LED em feridas de pacientes diabéticos. Conclusão: A luz LED mostrou-se benéfica no reparo tecidual, com aumento na produção de colágeno e fibroblastos, angiogênese, redução da inflamação e consequentemente, diminuição no tamanho da lesão.

https://doi.org/10.1590/1983-1447.2023.20220274.en

Physiological responses during walking in men and women with intermittent claudication
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Miyasato, Roberto Sanches

; Felix, Alex Jesus

; Andrade-Lima, Aluísio

; Silva Júnior, Natan Daniel da

; Ritti-Dias, Raphael Mendes

; Wolosker, Nelson

; Cornelissen, Véronique

; Goessler, Karla Fabiana

; Forjaz, Claúdia Lúcia de Moraes

einstein (São Paulo)

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2023, Volume 21 elocation eAO0120

Abstract: En | Text: En | PDF: En

ABSTRACT Objective Peak oxygen consumption (VO2peak), anaerobic threshold, walking economy, and cardiovascular responses during walking are used to guide and monitor walking training in patients with peripheral artery disease and intermittent claudication. Women with peripheral artery disease and intermittent claudication present greater impairments than men, and evaluating training markers according to sex for decisions regarding walking prescription in this population is important. This study aimed to compare VO2peak, walking economy, anaerobic threshold, and cardiovascular responses during walking in men and women with peripheral artery disease and intermittent claudication. Methods Forty patients (20 men and 20 women with similar baseline characteristics) underwent a cardiopulmonary treadmill test (3.2km/h and 2% increase in slope every 2 minutes until maximal leg pain). The VO2 and rate-pressure product were assessed. Data from men and women were compared using t-tests. Results There were no significant differences between men and women (VO2peak: 15.0±4.8 versus 13.9±2.9mL∙kg-1∙min-1, p=0.38; walking economy: 9.6±2.7 versus 8.4±1.6mL∙kg-1∙min-1, p=0.09; anaerobic threshold: 10.5±3.2 versus 10.5±2.2mL∙kg-1∙min-1, p=0.98; rate pressure product at 1st stage: 13,465± 2,910 versus 14,445±4,379bpm∙mmHg, p=0.41; and rate pressure product at anaerobic threshold:13,673±3,100 versus 16,390±5,870bpm∙mmHg, p=0.08 and rate pressure product at peak exercise: 21,253±6,141 versus 21,923±7,414bpm∙mmHg, p=0.76, respectively). Conclusion Men and women with peripheral artery disease and similar baseline characteristics presented similar responses to walking, suggesting that decisions regarding walking prescription and monitoring can be made regardless of sex in this specific population.

https://doi.org/10.31744/einstein_journal/2023ao0120

Capitalismo patriarcal em tempos de antropoceno: reflexões sobre a distopia feminista Deuses de Pedra
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Martins, Mariana Link

; Dias, Jenifer da Silva

; Fonseca, Cláudia Lorena Vouto da

Gragoatá

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2023, Volume 28 N. 61 elocation e56358

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT: This paper analyzes the novel The Stone Gods (2012) by the British author Jeanette Winterson as a feminist dystopia that presents the Anthropocene and its urgencies as a thread of tragic stories that intertwine in different space-times. Considered as sf, in the sense that it is equivalent to science fiction, speculative fabulation, and speculative feminism (HARAWAY, 2016), the apocalyptic plot of the narrative manifests clear criticism of capitalist and patriarchal relations of oppression, exposing them as responsible for the catastrophic course humanity has taken, as well as speculating on possibilities of resistance to destabilize such structures. Based on these assumptions, the analysis is founded on a feminist critique grounded on the reflections of Silvia Federici (2019), articulating the relations of race, gender, and sexuality in the constitution of the capitalist exploitation model, and to observe the effects that these structures cause in nature as a result of human intervention.

RESUMO: Com base em uma visão ecocrítica feminista, este artigo analisa o romance Deuses de Pedra (2012), da autora britânica Jeanette Winterson, como uma distopia feminista que retrata o Antropoceno e suas urgências como um fio condutor de histórias trágicas que se entrelaçam em diferentes espaços-tempos. Entendida como sf, no sentido de equivaler à ficção científica, fabulação especulativa e feminismo especulativo (HARAWAY, 2016), a narrativa de enredo apocalíptico manifesta claras críticas às relações de opressão capitalistas e patriarcais, expondo-as como responsáveis pelo rumo catastrófico que a humanidade tomou, bem como especula sobre as possibilidades de resistência para desestabilizar tais estruturas. Partindo destes pressupostos, o estudo tem por base uma crítica feminista fundamentada, sobretudo, nas reflexões de Silvia Federici (2019), articulando as relações de raça, gênero e sexualidade na constituição do modelo de exploração capitalista, além de observar os efeitos que essas estruturas causam na natureza, por meio da intervenção dos humanos.

https://doi.org/10.22409/gragoata.v28i61.56358.pt

Common mental disorders among medical students: systematic review and meta-analysis of Brazilian studies
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Soares, Silvio José Batista

; Fernandes, Cláudia Fernanda Garcez

; Tabalipa, Renata

; Kogima, Felipe

; Jubini, Marcelo Augusto Moreira

; Dias, Isabella Martins Vieira

; Soares, Victor Emanuel Miranda

; Amaral, Severina Silva

; Cruz, Michele Santos da

; Guerra, Paulo Henrique

Sao Paulo Medical Journal

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aug 2022, Volume 140 N. 4 Pages 615 - 622

Abstract: En | Text: En | PDF: En

ABSTRACT BACKGROUND: Common mental disorders (CMDs) have been correlated with consequences in different domains of life. OBJECTIVE: To summarize the prevalence rates of CMDs and factors associated with them among students at Brazilian medical schools. DESIGN AND SETTING: Systematic review and meta-analysis of studies developed in Brazilian medical schools. METHODS: In October 2021, searches were carried out in seven electronic databases, in Google Scholar and in reference lists. Observational studies reporting prevalence rates of CMDs among students at Brazilian medical schools were sought. Variables associated with CMDs arising from multivariate regression models were included in the synthesis. A meta-analysis was developed using a random-effects model and the risk of bias was assessed using an instrument developed from previous references. RESULTS: Fourteen original studies were included. The pooled prevalence rate of CMDs among undergraduate students at Brazilian medical schools was 43.3% (95% confidence interval = 38.9% to 47.6%; I2 = 87%; n = 3,927). Among the nine studies in which multivariate analyses were conducted, five showed risk associations between CMDs and medical school-related dissatisfactions, among which the desire to abandon the medical course can be highlighted (n = 3). In three studies, CMDs were associated with sleep indicators. CONCLUSION: Considering that the prevalence of CMDs among medical students is higher than in the general population, we recommend that Brazilian medical schools should give greater attention to this topic, and should enable expansion of care offerings relating to mental health. SYSTEMATIC REVIEW REGISTRATION: Prospective Register of Systematic Reviews (PROSPERO) database (CRD42020142184).

https://doi.org/10.1590/1516-3180.2021.0851.r1.27012022

Use of P-CPQ to measure the impact of oral health on the quality of life of children with special health care needs
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Dias-Caldeira, François Isnaldo

; Baeta-de-Oliveira, Luísa

; Bintencourt-Reis, Caio Luiz

; Pedreira-de-Almeida, Ana Cláudia

; Alves-Nogueira, Denismar

; Coelho-de-Lima, Daniela

; Barroso-de-Oliveira, Daniela Silva

CES Odontología

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jan 2022, Volume 35 N. 1 Pages 17 - 30

Abstract: En Pt Es | Text: En Pt Es | PDF: En | PDF: Pt | PDF: Es

Abstract Introduction and objective: the WHO defined the concept of quality of life as the self-perception that the individual has about his position in the social, cultural and ideological context, being these factors the main responsible for denoting the worst parameters regarding oral health care during the dental clinical examination performed by the dentist. To evaluate the impact of oral health on the quality of life of children with special health care needs aged 7 to 14 years old and the influence of different types of specialties on the quality of life, in the view of parents/caregivers in the Brazil southeast region. Materials and methods: the sample was composed of 62 children enrolled in municipal public schools and the Association of Parents and Friends of the Exceptional. The validated Parental- Caregivers Perception Questionnaire (P-CPQ) was used as an instrument for data collection, applied in the home environment and answered by the main responsible. The relation between demographic factors, type of special need and P-CPQ was evaluated with alpha of 0.05. Results: the worst parameters in Family Impact Scale were observed when the mother and father was responsible for data transmission (p= 0.004). Oral health was associated with the worst parameters of oral symptoms on quality of life in the view of parents (p = 0.012). There was no statistically significant difference regarding the impact of the child's type of specialty on quality of life. Conclusion: the variables caregiver and oral health status child are related to the worst indicators regarding the impact of oral health on quality of life.

Resumo Introdução e objetivo: a OMS definiu o conceito de qualidade de vida como a autopercepção que o indivíduo tem sobre sua posição no contexto social, cultural e ideológico, sendo esses fatores os principais responsáveis por denotar os piores parâmetros quanto aos cuidados com a saúde bucal durante o exame clínico odontológico realizada pelo dentista. Avaliar o impacto da saúde bucal na qualidade de vida de crianças com necessidades especiais de cuidados de saúde de 7 a 14 anos de idade e a influência de diferentes tipos de especialidades na qualidade de vida, na visão dos pais/cuidadores na região sudeste do Brasil. Materiais e métodos: a amostra foi composta por 62 crianças matriculadas nas escolas públicas municipais e na Associação de Pais e Amigos dos Excepcionais. Foi utilizado o Parental- Caregivers Perception Questionnaire (P-CPQ) como um instrumento de mensuração, aplicado no ambiente familiar e respondido pelo responsável principal. A relação entre fatores demográficos, tipo de necessidade especial e o P-CPQ foi avaliada com alfa de 0,05. Resultados: os piores parâmetros em relação à qualidade de vida foram observados quando a mãe foi a responsável pela transmissão dos dados (p= 0,004). A variável estado de saúde bucal (p= 0,012) apresentou resultados relevantes quando relacionadas com os piores parâmetros na percepção do impacto da saúde bucal na qualidade de vida na percepção dos cuidadores sobre suas crianças. Não houve diferença estatisticamente significante com relação ao impacto do tipo de especialidade da criança na qualidade de vida. Conclusão: as variáveis cuidador e estado de saúde oral da criança estão relacionadas com os piores indicadores relativos ao impacto da saúde oral sobre a qualidade de vida.

Resumen Introducción y objetivo: la OMS definió el concepto de calidad de vida como la autopercepción que tiene el individuo sobre su posición en el contexto social, cultural e ideológico, siendo estos factores los principales responsables de denotar los peores parámetros en cuanto al cuidado de la salud bucal durante el examen clínico dental realizado por el dentista. Evaluar el impacto de la salud bucal en la calidad de vida de los niños con necesidades especiales de atención de la salud de 7 a 14 años de edad y la influencia de los diferentes tipos de especialidades en la calidad de vida, en opinión de los padres/cuidadores de la región sudoriental del Brasil. Materiales y métodos: la muestra se compuso de 62 niños matriculados en escuelas públicas municipales y en la Asociación de Padres y Amigos de los Excepcionales. Se utilizó el cuestionario validado Parental- Caregivers Perception Questionnaire (P-CPQ) como instrumento para la reunión de datos, aplicado en el entorno doméstico y contestado por el principal responsable. La relación entre los factores demográficos, el tipo de necesidad especial y el P-CPQ se evaluó con un alfa de 0,05. Resultados: los peores parámetros en la Escala de Impacto Familiar se observaron cuando la madre y el padre fueron los responsables de la transmisión de datos (p= 0,004). La salud oral se asoció con los peores parámetros de los síntomas orales sobre la calidad de vida a juicio de los padres (p = 0,012). No hubo diferencias estadísticamente significativas en cuanto al impacto del tipo de especialidad del niño en la calidad de vida. Conclusión: las variables "cuidador" y "estado de salud bucal del niño" se relacionan con los peores indicadores relativos al impacto de la salud bucal en la calidad de vida.

https://doi.org/10.21615/cesodon.6010

Scrotal hemangiosarcoma in a Large White boar
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Silveira, Renato Luiz

; Cruz, Ana Claudia de Menezes

; Doria, Phillipe Bauer de Araújo

; Silveira, Joao Marcelo Silva

; Vasconcelos, Carlos Otávio de Paula

; Rodrigues, André Luis Rios

; Arashiro, Eduardo Kenji Nunes

; Jerdy, Hassan

; Dias, Mariana Tavares

; Carvalho, Eulógio Carlos Queiroz de

; Costa, Carlos Henrique Campello

Ciência Rural

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2022, Volume 52 N. 4 elocation e20210157

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Na espécie suína os tumores são pouco diagnosticados devido a curta vida útil dos animais de produção. Normalmente não causam sinais clínicos, sendo, muitas vezes, encontrados no momento do abate. Um reprodutor com dois anos de idade, da raça Large White, utilizado no manejo reprodutivo de uma granja, sem histórico de problemas pré-existentes, foi examinado em função de lesões na pele da bolsa escrotal. Foram coletadas amostras de segmentos de pele contendo lesões para o diagnóstico histopatológico e imunohistoquímico. Microscopicamente os nodos em bolsa escrotal consistiam em neoplasias mal demarcadas, altamente celulares, expansivas e multifocalmente invasivas composta por endoteliócitos imaturos organizados em formações neovasculares. As células tumorais eram pleomórficas, levemente ovais a fusiformes com citoplasma eosinofílico e núcleos hipercromáticos e com um a três nucléolos. Todos os nódulos analisados foram compatíveis com hemangiossarcoma. Para a quantificação da angiogênese tecidual, após a avaliação imunohistoquímica, as células neoplásicas imunoexpressaram os anticorpos monoclonais CD31 e Fator VIII, através da identificação de proteínas expressas na superfície das células endoteliais. Observou-se o marcador de proliferação celular Ki67 positivo em aproximadamente 10% das células neoplásicas, demonstrando um alto grau de malignidade. O hemangiossarcoma na espécie suína já foi identificado em vários órgãos e tecidos, mas, até o momento, nenhum trabalho demonstrou o diagnóstico na pele da bolsa escrotal, como reportado, ineditamente, neste estudo. Face o exposto, espera-se que este relato contribua para a frequência de neoplasias encontradas na espécie suína.

ABSTRACT: Tumors are rarely diagnosed in swine specie because of the short lifespan of production animals. Normally, these tumors do not present any clinical signs and are often detected at the time of slaughter. A 2-year-old Large White boar, used in the reproductive management of a farm and without a history of pre-existing problems, was examined for skin lesions on the scrotum. Samples were collected from skin segments containing lesions for histopathological and immunohistochemical diagnosis. Microscopically, the nodes in the scrotum pouch consisted of poorly demarcated, highly cellular, expansile, and multifocally invasive neoplasms, composed of immature endotheliocytes organized into neovascular formations. The tumor cells were pleomorphic, slightly oval to spindle-shaped, with eosinophilic cytoplasm and hyperchromatic nuclei with one to three nucleoli. All the nodules analyzed were compatible with hemangiosarcoma. After immunohistochemical evaluation, for the quantification of tissue angiogenesis the neoplastic cells immunoexpressed the CD31 monoclonal antibodies and factor VIII, through the identification of proteins expressed on the surface of endothelial cells. The Ki67 cell proliferation marker was positive in approximately 10% of the neoplastic cells, demonstrating a high degree of malignancy. Hemangiosarcoma in swine species has already been identified in several organs and tissues; however, to date, no study has demonstrated the diagnosis of this condition on the skin of the scrotum, as reported in this study. Therefore, it is expected that this report will contribute to the knowledge of the frequency of neoplasms in swine species.

https://doi.org/10.1590/0103-8478cr2021015 69 downloads

10.

The moderation of institutional mimicry on eco-innovation performance: evidence from Brazil
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Silva, José Jaconias da

; Cirani, Claudia Brito Silva

; Dias, Helison Bertoli Alves

; Lima, Ana Maria de

Revista Brasileira de Inovação

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2022, Volume 21 elocation e022004

Abstract: En | Text: En | PDF: En

ABSTRACT The moderating force that institutional pressures can exert on the relationship between eco-innovation and performance has received little attention. In In this context, the present study aims to investigate the relationship between eco-innovation and environmental and financial performance, as well as the moderating role of institutional mimetic pressure in this relationship. We surveyed 175 brazilian companies from the organic production sector. For the analysis, we used Structural Equation Modeling to verify the relationship between the variables. The main results point to (i) a positive relationship between eco-innovation and environmental performance; (ii) the relationship between eco-innovation and financial performance does not occurs directly, but indirectly, due to environmental performance; and (iii) mimetic pressure attenuates the influence of eco-innovation on environmental and financial performance. This finding had not yet been verified in the literature on innovation, an unprecedented contribution of this work. Therefore, business managers can use the findings to implement innovation management systems under eco-innovative perspectives to produce goods with less environmental impact.

https://doi.org/10.20396/rbi.v21i00.8664065

11.

Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022
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Claro, Ingra Morales

; Romano, Camila Malta

; Candido, Darlan da Silva

; Lima, Evelyn Lepka de

; Lindoso, José Angelo Lauletta

; Ramundo, Mariana Severo

; Moreira, Filipe Romero Rebello

; Barra, Luiz Alberto Costa

; Borges, Luciana Marques Sansão

; Medeiros, Lucas Alberto

; Tomishige, Marcia Y. S.

; Moutinho, Tomas

; Silva, Anderson José Dias da

; Rodrigues, Camila Cristina Martini

; Azevedo, Luiz Cesar Fernandes de

; Villas-Boas, Lucy Santos

; Silva, Camila Alves Maia da

; Coletti, Thaís Moura

; Manuli, Erika R.

; O’Toole, Aine

; Quick, Joshua

; Loman, Nicholas

; Rambaut, Andrew

; Faria, Nuno R.

; Figueiredo-Mello, Claudia

; Sabino, Ester Cerdeira

Revista do Instituto de Medicina Tropical de São Paulo

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2022, Volume 64 elocation e48

Abstract: En | Text: En | PDF: En

ABSTRACT Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.

https://doi.org/10.1590/s1678-9946202264048

12.

Fast surveillance response reveals the introduction of a new yellow fever virus sub-lineage in 2021, in Minas Gerais, Brazil
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Andrade, Miguel Souza

; Campos, Fabrício Souza

; Oliveira, Cirilo Henrique de

; Oliveira, Ramon Silva

; Campos, Aline Alves Scarpellini

; Almeida, Marco Antônio Barreto de

; Fonseca, Vagner de Souza

; Simonini-Teixeira, Danilo

; Sevá, Anaiá da Paixão

; Temponi, Andrea Oliveira Dias

; Magalhães, Fernando Maria

; Chaves, Danielle Costa Capistrano

; Pereira, Maira Alves

; Lamounier, Ludmila Oliveira

; Menezes, Givaldo Gomes de

; Aquino-Teixeira, Sandy Micaele

; Gonçalves-dos-Santos, Maria Eduarda

; Bernal-Valle, Sofía

; Müller, Nicolas Felipe Drumm

; Cardoso, Jader da Cruz

; Santos, Edmilson dos

; Mares-Guia, Maria Angélica

; Albuquerque, George Rêgo

; Romano, Alessandro Pecego Martins

; Franco, Ana Cláudia

; Ribeiro, Bergmann Morais

; Roehe, Paulo Michel

; Abreu, Filipe Vieira Santos de

Memórias do Instituto Oswaldo Cruz

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2022, Volume 117 elocation e220127

Abstract: En | Text: En | PDF: En

BACKGROUND In Brazil, the yellow fever virus (YFV) is maintained in a sylvatic cycle involving wild mosquitoes and non-human primates (NHPs). The virus is endemic to the Amazon region; however, waves of epidemic expansion reaching other Brazilian states sporadically occur, eventually causing spillovers to humans. OBJECTIVES To report a surveillance effort that led to the first confirmation of YFV in NHPs in the state of Minas Gerais (MG), Southeast region, in 2021. METHODS A surveillance network was created, encompassing the technology of smartphone applications and coordinated actions of several research institutions and health services to monitor and investigate NHP epizootics. FINDINGS When alerts were spread through the network, samples from NHPs were collected and YFV infection confirmed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and genome sequencing at an interval of only 10 days. Near-complete genomes were generated using the Nanopore MinION sequencer. Phylogenetic analysis indicated that viral genomes were related to the South American genotype I, clustering with a genome detected in the Amazon region (state of Pará) in 2017, named YFVPA/MG sub-lineage. Fast YFV confirmation potentialised vaccination campaigns. MAIN CONCLUSIONS A new YFV introduction was detected in MG 6 years after the beginning of the major outbreak reported in the state (2015-2018). The YFV strain was not related to the sub-lineages previously reported in MG. No human cases have been reported, suggesting the importance of coordinated surveillance of NHPs using available technologies and supporting laboratories to ensure a quick response and implementation of contingency measures to avoid YFV spillover to humans.

https://doi.org/10.1590/0074-02760220127

13.

SEED TREATMENT WITH TRICHODERMA AND CHEMICALS TO IMPROVE PHYSIOLOGICAL AND SANITARY QUALITY OF WHEAT CULTIVARS1
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COUTO, ANA PAULA SILVA

; PEREIRA, ALANA EMANOELE

; ABATI, JULIA

; FONTANELA, MAIRA LAÍZA CAMARGO

; DIAS-ARIEIRA, CLAUDIA REGINA

; KROHN, NÁDIA GRACIELE

Revista Caatinga

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dec 2021, Volume 34 N. 4 Pages 813 - 823

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O tratamento de sementes com fungos do gênero Trichoderma spp. proporciona diversos benefícios, incluindo promoção de crescimento das plantas, tolerância a estresses e controle de fungos patogênicos. Entretanto, para evitar o uso de doses inadequadas e custos desnecessários, é fundamental a utilização destes na quantidade correta, todavia não há estudos que avaliem a sua aplicabilidade em sementes de trigo. Objetivou-se encontrar a dose mais eficiente de produtos a base de Trichoderma no tratamento de sementes para melhorar a qualidade fisiológica e sanitária das cultivares de trigo TBIO 'Toruk' e TBIO 'Sossego', além de comparar o desempenho de agentes biológicos e químicos. Os tratamentos biológicos utilizados foram: Trichoderma asperellum SF 04 e Trichoderma harzianum IBLF006, aplicados nas doses de zero (testemunha); 5x1011; 1x1012; 1,5x1012 e 2x1012 UFC (unidades formadoras de colônias) 100 kg-1 de sementes, e os químicos foram: carboxina + tiram e piraclostrobina + tiofanato metílico + fipronil. Em laboratório, conduziu-se testes de germinação, comprimento e massa de matéria seca de plântulas, blotter test e, em casa de vegetação, emergência, comprimento e massa de matéria seca da parte aérea de plântulas. Os tratamentos biológicos T. asperellum SF 04 e T. harzianum IBLF006 apresentam melhor eficiência para aplicação no tratamento de sementes de trigo na dose 2x1012 UFC 100 kg-1 de sementes. Comparando os produtos biológicos com os químicos, os dados indicam que ambas as opções têm potencial para serem utilizadas no manejo de doenças do trigo, além de promoverem o crescimento de plântulas, por meio do tratamento de sementes.

ABSTRACT Seed treatment with fungi of the genus Trichoderma spp. provides several benefits, including plant growth promotion, stress tolerance, and pathogenic fungi control. Moreover, to avoid inadequate doses and unnecessary costs, these treatments must be applied in proper amounts. However, no study has evaluated their applicability in wheat seeds. This study aimed to determine the most efficient dose of Trichoderma-based products applied as a seed treatment for improving the physiological and sanitary quality of the wheat cultivars TBIO ‘Toruk’ and TBIO ‘Sossego’, besides comparing the performance of biological and chemical agents. Two biological treatments (Trichoderma asperellum SF 04 and Trichoderma harzianum IBLF006) were applied at 0 (control), 5 × 10 11 , 1 × 10 12 , 1.5 × 10 12 , and 2 × 10 12 colony-forming units (CFU) 100 kg–1 seed. Two chemical treatments (carboxin + thiram and pyraclostrobin + thiophanate-methyl + fipronil) were applied at the manufacturers’ recommended doses. Seed germination, shoot and root lengths, seedling dry matter, and sanitary quality were analyzed under laboratory conditions, while seedling emergence, shoot length, and shoot dry matter were analyzed under greenhouse conditions. The optimal dose for wheat seed treatment with T. asperellum SF 04 and T. harzianum IBLF006 was 2 × 10 12 CFU 100 kg–1 seed. When comparing biological and chemical products, our findings indicate that both options are adequate for managing wheat diseases and providing seedling growth via seed treatment.

https://doi.org/10.1590/1983-21252021v34n408rc 36 downloads

14.

Brachiaria grasses in vitro digestibility with bovine and ovine ruminal liquid as inoculum
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Itavo, Luis Carlos Vinhas

; Ítavo, Camila Celeste Brandão Ferreira

; Valle, Cacilda Borges do

; Dias, Alexandre Menezes

; Difante, Gelson dos Santos

; Morais, Maria da Graça

; Soares, Claudia Muniz

; Pereira, Camila da Silva

; Oliveira, Ronaldo Lopes

Revista mexicana de ciencias pecuarias

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dec 2021, Volume 12 N. 4 Pages 1045 - 1060

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Se planteó la hipótesis de que es posible que el inóculo de diferentes especies de rumiantes con capacidades digestivas diferentes que se alimentan de un determinado forraje puede mostrar diferentes utilizaciones de alimento en comparación con otras especies de rumiantes. Se evaluaron cinco gramíneas Brachiaria: B. decumbens cv. Basilisk, B. decumbens acceso D70, B. humidicola cv. Tupi, B. humidicola cv. Común, y B. ruziziensis acceso R124, en dos edades de rebrote (21 y 42 d). Se analizó la producción, el contenido bromatológico, la digestibilidad de materia seca in vitro (DMSiv) y la digestibilidad de la fibra detergente neutro in vitro (DFDNiv) utilizando inóculos bovinos u ovinos. El experimento utilizó un diseño factorial de 5 × 2 × 2 y encontró efectos significativos para la variedad de gramínea y la edad de rebrote. Además, se encontraron interacciones significativas de gramínea × edad en la materia seca, la proteína cruda, la fibra detergente neutro y la fibra detergente ácido de la muestra total y lamina de la hoja. Hubo un efecto significativo de la variedad de gramínea y la edad de la gramínea en la masa del forraje, la relación lámina de la hoja/tallo, lámina de la hoja, tallo, material senescente y crecimiento. En los ensayos de la digestibilidad in vitro, el origen del inóculo mostró un efecto significativo en algunas variedades. Debido a las diferencias en los ensayos in vitro, se recomendó el uso de inóculos específicos de las especies para las evaluaciones de alimentos según el animal al que se destina. Asimismo, B. decumbens cv. Basilisk presentó la mejor digestibilidad in vitro (DMSiv y DFDNiv) en inóculo bovino, mientras que B. humidicola cv. Tupi tuvo mejor digestibilidad in vitro (DMSiv y DFDNiv) en inóculo ovino.

Abstract It was hypothesized that it is possible that inoculum from different ruminant species with different digestive abilities feeding from a certain forage may show different feed utilizations comparing to other ruminant species. Five Brachiaria grasses were evaluated: B. decumbens cv. Basilisk, B. decumbens access D70, B. humidicola cv. Tupi, B. humidicola cv. Common, and B. ruziziensis access R124, at two regrowth ages (21 and 42 d). Production, bromatological content, in vitro dry matter digestibility (ivDMD) and in vitro neutral detergent fiber digestibility (ivNDFD) were analyzed using bovine or ovine inoculums. The experiment used a 5 × 2 × 2 factorial design and found significant effects for grass variety and regrowth age. In addition, significant interactions from grass × age on dry matter, crude protein, neutral detergent fiber and acid detergent fiber of total sample and leaf blade were found. There were significant effect of grass variety and grass age on forage mass, leaf blade/stem ratio, leaf blade, stem, senescent material and growth. In vitro digestibility assays of inoculum source showed significant effect in some varieties. Due to differences in in vitro assays, it was recommended the use of species-specific inoculums for feed evaluations according to the animal it is intended for. Also, B. decumbens cv. Basilisk presented the best in vitro digestibility (ivDMD and ivNDFD) in bovine inoculum, whereas B. humidicola cv. Tupi had better in vitro digestibility (ivDMD and ivNDFD) in ovine inoculum.

https://doi.org/10.22319/rmcp.v12i4.5294 35 downloads

15.

Clinical characteristics and outcomes of COVID-19 patients admitted to the intensive care unit during the first year of the pandemic in Brazil: a single center retrospective cohort study
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Corrêa, Thiago Domingos

; Midega, Thais Dias

; Timenetsky, Karina Tavares

; Cordioli, Ricardo Luiz

; Barbas, Carmen Silvia Valente

; Silva Júnior, Moacyr

; Bravim, Bruno de Arruda

; Silva, Bruno Caldin

; Matos, Gustavo Faissol Janot de

; Nawa, Ricardo Kenji

; Carvalho, Fabrício Rodrigues Torres de

; Queiroz, Verônica Neves Fialho

; Rabello Filho, Roberto

; Piza, Felipe Maia de Toledo

; Pereira, Adriano José

; Pesavento, Marcele Liliane

; Eid, Raquel Afonso Caserta

; Santos, Bento Fortunato Cardoso dos

; Pardini, Andreia

; Teich, Vanessa Damázio

; Laselva, Claudia Regina

; Cendoroglo Neto, Miguel

; Klajner, Sidney

; Ferraz, Leonardo José Rolim

einstein (São Paulo)

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2021, Volume 19 elocation eAO6739

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Descrever características clínicas, uso de recursos e desfechos e identificar preditores de mortalidade intra-hospitalar de pacientes com COVID-19 admitidos na unidade de terapia intensiva. Métodos: Estudo de coorte retrospectivo, em centro único, realizado em um hospital privado localizado em São Paulo (SP). Pacientes adultos (≥18 anos) admitidos consecutivamente na unidade de terapia intensiva, entre 4 de março de 2020 a 28 de fevereiro de 2021, foram incluídos neste estudo. Os pacientes foram classificados como sobreviventes e não sobreviventes, de acordo com a alta hospitalar. Resultados: Durante o período do estudo, 1.296 pacientes [mediana (intervalo interquartil) de idade: 66 (53-77) anos] com COVID-19 foram admitidos na unidade de terapia intensiva. Destes, 170 (13,6%) pacientes morreram no hospital (não sobreviventes), e 1.078 (86,4%) receberam alta hospitalar (sobreviventes). Comparados aos sobreviventes, os não sobreviventes eram mais idosos [80 (70-88) versus 63 (50-74) anos; p<0,001], apresentavam pontuação mais alta no sistema prognóstico Simpliﬁed Acute Physiology Score 3 [59 (54-66) versus 47 (42-53); pontos p<0,001] e tinham mais comorbidades. Durante a internação na unidade de terapia intensiva, 56,6% dos pacientes usaram ventilação não invasiva, 32,9% usaram ventilação mecânica invasiva, 31,3% usaram cateter nasal de alto fluxo, 11,7% foram submetidos à terapia renal substitutiva, e 1,5% usou oxigenação por membrana extracorpórea. Os preditores independentes de mortalidade intra-hospitalar foram idade, Sequential Organ Failure Assessment, Índice de Comorbidade de Charlson, necessidade de ventilação mecânica, uso de cateter nasal de alto fluxo, uso de terapia renal substitutiva e suporte por oxigenação por membrana extracorpórea. Conclusão: Pacientes com quadros graves da COVID-19 admitidos na unidade de terapia intensiva apresentaram considerável mortalidade e morbidade, com alta demanda de terapia de suporte e internação prolongada em unidade de terapia intensiva e hospitalar.

ABSTRACT Objective: To describe clinical characteristics, resource use, outcomes, and to identify predictors of in-hospital mortality of patients with COVID-19 admitted to the intensive care unit. Methods: Retrospective single-center cohort study conducted at a private hospital in São Paulo (SP), Brazil. All consecutive adult (≥18 years) patients admitted to the intensive care unit, between March 4, 2020 and February 28, 2021 were included in this study. Patients were categorized between survivors and non-survivors according to hospital discharge. Results: During the study period, 1,296 patients [median (interquartile range) age: 66 (53-77) years] with COVID-19 were admitted to the intensive care unit. Out of those, 170 (13.6%) died at hospital (non-survivors) and 1,078 (86.4%) were discharged (survivors). Compared to survivors, non-survivors were older [80 (70-88) versus 63 (50-74) years; p<0.001], had a higher Simpliﬁed Acute Physiology Score 3 [59 (54-66) versus 47 (42-53) points; p<0.001], and presented comorbidities more frequently. During the intensive care unit stay, 56.6% of patients received noninvasive ventilation, 32.9% received mechanical ventilation, 31.3% used high flow nasal cannula, 11.7% received renal replacement therapy, and 1.5% used extracorporeal membrane oxygenation. Independent predictors of in-hospital mortality included age, Sequential Organ Failure Assessment score, Charlson Comorbidity Index, need for mechanical ventilation, high flow nasal cannula, renal replacement therapy, and extracorporeal membrane oxygenation support. Conclusion: Patients with severe COVID-19 admitted to the intensive care unit exhibited a considerable morbidity and mortality, demanding substantial organ support, and prolonged intensive care unit and hospital stay.

https://doi.org/10.31744/einstein_journal/2021ao6739

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