ABSTRACT Callosobruchus maculatus (Fab.) (Coleoptera: Chrysomelidae: Bruchinae) is the main pest of Vigna unguiculata (L.) Walp. during the grain storage period. This study aimed to report the occurrence of a natural enemy associated with C. maculatus in cowpea, in the semiarid region of the Rio Grande do Norte state, Brazil. For this, cowpea samples were collected and placed in closed plastic containers, where they remained until the emergence of adult insects. From the infested samples, adults of C. maculatus and of the Dinarmus basalis (Rondani) (Hymenoptera: Pteromalidae: Pteromalinae) parasitoid emerged. This is the first report of D. basalis parasitizing C. maculatus in the semiarid region of the Rio Grande do Norte state.

RESUMO Callosobruchus maculatus (Fab.) (Coleoptera: Chrysomelidae: Bruchinae) é a principal praga de Vigna unguiculata (L.) Walp. durante o período de armazenamento dos grãos. Objetivou-se relatar a ocorrência de um inimigo natural associado a C. maculatus em feijão-caupi, na região semiárida do Rio Grande do Norte, Brasil. Para isso, amostras de feijão-caupi foram coletadas e colocadas em recipientes plásticos fechados, onde permaneceram até a emergência dos insetos adultos. Das amostras infestadas, emergiram adultos de C. maculatus e do parasitoide Dinarmus basalis (Rondani) (Hymenoptera: Pteromalidae: Pteromalinae). Este é o primeiro relato de D. basalis parasitando C. maculatus na região semiárida do Rio Grande do Norte.

ABSTRACT: This work is the first record of Encarsia inaron (Walker, 1839) (Hymenoptera: Aphelinidae) parasitizing Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) on soybeans in Brazil. Morphological and molecular characterizations are provided, in addition to verification of the presence of E. inaron in areas infested by whiteflies, indicating that the wasp can potentially be used in integrated management programs. Additionally to morphological verification, the molecular analysis allowed the identification of some degree of genetic variability in the genome of the parasitoid.

RESUMEN: Este es el primer registro de Encarsia inaron (Walker, 1839) (Hymenoptera: Aphelinidae) parasitando Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) en soja en Brasil. Se proporcionan caracterizaciones morfológicas y moleculares, además de la verificación de la presencia de E. inaron en áreas infestadas por mosca blanca, lo que indica que la avispa puede potencialmente ser utilizada en programas de manejo integrado. Además de la verificación morfológica, el análisis molecular permitió identificar algún grado de variabilidad genética en el genoma del parasitoide.

ABSTRACT The parasitism of Acanthoscelides macrophthalmus (Coleoptera, Chrysomelidae, Bruchinae) by the parasitoid Horismenus abnormicaulis (Hymenoptera: Eulophidae) is described for the first time. We harvested 90 Leucaena leucocephala (Fabaceae, Mimosoideae) fruit pods, in June 2019 on the campus of the Federal University of Lavras in the municipality of Lavras, Minas Gerais, Brazil. We found 21 individuals of H. abnormicaulis and 334 of A. macrophthalmus. The presence of cephalic capsule of beetle's pupa and evidence of predation into seed pods from which the wasps emerged suggested H. abnormicaulis is a parasitoid of A. macrophthalmus. Horismenus abnormicaulis has been added to the list of A. macrophthalmus parasitoids, and its occurrence has been expanded to the state of Minas Gerais. This discovery expands our knowledge of H. abnormicaulis biology and distribution in Brazil, as well as the tri-trophic interaction, plant-herbivore-parasitoid in invasive plant systems.

Abstract Aim: To evaluate the intensity of training and official badminton matches (international championship) in junior elite players. Methods: Twelve elite players from the Brazilian junior national team (6 male and 6 female) were monitored during 19 training sessions and 50 official men's and women's single and double matches in the XXVII Pan-American Junior Games. Subjects underwent an incremental running step test to determine maximal oxygen uptake (VO2max), and an individual relationship between heart rate (HR) and VO2max was established to estimate exercise intensity and oxygen uptake at official matches (OMs) and training sessions. HR was monitored during multi-shuttlecock (MS), technical-tactical (TT), and physical (PS) training methods, as well as during simulated matches (SMs) and OMs. Variables such as %HRmax, HR zones, %VO2max, and energy expenditure were also calculated. Results: OM was the most intense activity monitored for male and female athletes, followed by SM according to %HRmax (86.8 ± 4.1% and 84.4 ± 5.0% for female and male, respectively, in OM vs. 74.6 ± 2.3% and 75.0 ± 5.0% for female and male, respectively, in SM). OM mean energy expenditure was 10.7 ± 0.5 kcal.min-1 for females and 14.9 ± 4.6 kcal.min−1 for males. Conclusion: MS training has less physiological demand and OM presented higher intensity. SM, on the other hand, had the closest physiological demand to an OM.

RESUMO: Pachira aquatica (Malvaceae), conhecida como munguba, é cultivada para arborização de muitas cidades brasileiras. No campus de Seropédica da UFRRJ, observou-se o desfolhamento de um espécime de Pachira aquatica, com a presença de larvas, pré-pupas e pupas de besouro da subfamília Hyperinae, que foram criadas em laboratório até a emergência dos adultos; esses insetos foram identificados como pertencentes à espécie Phelypera griseofasciata Capiomont (Coleoptera: Curculionidae). Essas formas imaturas (n=86) foram novamente coletadas nesse mesmo espécime e criadas em laboratório para verificar a ocorrência de parasitismo. Foram obtidos 128 adultos de Jaliscoa nudipennis Bouček (Hymenoptera: Pteromalidae), responsáveis por 44,2% do parasitismo total. A razão sexual foi de 0,70, portanto, o maior número de descendentes foi de fêmeas. Observaram-se ainda os dimorfismos inter e intrasexuais em J. nudipennis quanto ao formato da cabeça e às características das asas. Este estudo trata-se do primeiro registro de ambas as espécies de insetos associadas a Pachira aquatica no estado do Rio de Janeiro, Brasil.

ABSTRACT: Pachira aquatica (Malvaceae), known as munguba, is cultivated for afforestation of many Brazilian cities. In Seropédica campus of UFRRJ, it was observed the defoliation of one specimen of Pachira aquatica with presence of larvae, pre-pupae, and pupae of beetle of the subfamily Hyperinae, which were reared in laboratory until the emergence of the adults; these insects were identified as belonging to the species Phelypera griseofasciata Capiomont (Coleoptera: Curculionidae). These immature forms (n=86) were again collected in this same specimen and reared in laboratory to verify the occurrence of parasitism. It was obtained 128 adults of Jaliscoa nudipennis Bouček (Hymenoptera: Pteromalidae), responsible for 44.2% of total parasitism. The sex ratio was 0.70, so the highest number of offspring was of females. Intersexual and intrasexual dimorphisms were also observed in J. nudipennis regarding the shape of the head and characteristics of the wings. This study is the first record of both insect species associated with Pachira aquatica in the state of Rio de Janeiro, Brazil.

Abstract Fruit flies (Diptera: Tephritidae) are important pests that affect the fruit-growing worldwide. In the northeastern Brazil, where a semiarid climate predominates and the production of tropical fruits for export is concentrated, some fruit flies, including Anastrepha spp. and Ceratitis capitata (Wiedemann), are considered pests due to economic damage and quarantine restrictions. In several parts of the world, fruit fly population regulation is carried out with the support of hymenopteran parasitoids. In Brazil, some information exists about larval parasitoids of fruit flies, but little is known about pupal parasitoids of these tephritids, especially in Brazilian semiarid environments. Therefore, the main objective of this study was to know the pupal parasitoids associated with C. capitata in a semiarid environment in Brazil. The parasitoid survey was carried out in a semiarid region in the states of Rio do Norte and Ceará. To obtain the parasitoids, pupae (sentinels) of C. capitata from the laboratory, Applied Entomology Laboratory of Universidade Federal Rural do Semi-Árido, were exposed to natural parasitism in the field. Six parasitoid (Hymenoptera) species were obtained: Pachycrepoideus vindemmiae (Rondani), Spalangia simplex Perkins, Spalangia gemina Boucek, Spalangia leiopleura Gibson, and Spalangia impunctata Howard (Pteromalidae); Trichopria anastrephae Lima (Diapriidae). These are the first records of pupal parasitoids associated with the fruit fly C. capitata in Brazil.

ABSTRACT Aedes aegypti is one of the most important culicidae species for public health, since it transmits the viruses for yellow fever, dengue fever, chikungunya and zika. With the purpose of analyzing the effect of plant extracts on the oviposition of this vector in ovitraps, 100 ovitraps were set containing an attractive plant substrate and a pressed wooden palette vertically installed on the pot for female oviposition. The traps were randomly distributed in 10 neighborhoods of Crato, Ceará, Brazil. To determine the best extract, water from the supply network, distilled water, sewage and aqueous extracts of plant leaves from guava (Psidium guajava L.), papaya (Carica papaya L), cassava (Manihot esculenta Grantz), cashew tree (Anacardium occidentale L.), sorghum (Sorghum bicolor L., Moench) and grass hay (Cynodon nlemfluensis Vanderyst) were used. The substrates were prepared at the Laboratory of Agricultural Entomology at the Center of Agricultural Sciences and Biodiversity of the Federal University of Cariri (UFCA). Leaves were collected from the plants and crushed using a blender and then mixed with water at a concentration of 50 g of each substrate per liter of water. The material was stored for seven days in a dark room to facilitate the reactions and avoid the photoinactivation of active compounds. To determinate the best concentration, guava leaves were used subject to the same treatment of the first experiment, at concentrations of 0.5, 1; 5, 10, 25, 50, and 75 g of substrate per liter of water. Traps with sewage water showed the highest attractiveness for the oviposition of A. aegypti and the extract of guava leaves showed the highest percentage of egg attractiveness for the vector mosquito. These results can be useful to guide the adoption of new entomological surveillance and control methods of A. aegypti.

RESUMEN Aedes aegypti es una de las especies de culícidos más importantes para la salud pública, pues es transmisor de los virus de la fiebre amarilla, dengue, chikungunya y zika. Intentando analizar el efecto de extractos vegetales sobre la ovoposición de ese vector en trampas de ovoposición, fueron instaladas 100 trampas conteniendo en su interior sustrato atrayente de un vegetal y una paleta de madera prensada insertada en la posición vertical de la pared del vaso para que la hembra realice la postura. Las trampas fueron distribuidas, al azar, en 10 barrios de Crato-CE. Para determinar el mejor extracto fueron utilizadas agua de la red de suministro, agua destilada, desagüe y extractos acuosos de las hojas del guayabo (Psidium guajava L.), papayo (Carica papaya L.), mandioca (Manihot esculenta Crantz), anacardo (Anacardium occidentale L.), sorgo (Sorghum bicolor L., Moench) y heno de la gramínea (Cynodon nlemfluemsis Vanderyst). Los sustratos fueron preparados en el Laboratorio de Entomología Agrícola, en el Centro de Ciencias Agrarias y de Biodiversidad (CCAB), de la Universidad Federal de Cariri (UFCA). Las hojas fueron extraídas de la planta y enseguida, trituradas en una licuadora y mezcladas con agua en concentración de 50 g de cada sustrato por litro de agua. El material pasó siete días en ambiente oscuro para facilitar las reacciones y evitar la foto inactivación de los ingredientes activos. Para determinar la mejor concentración fueron utilizadas hojas del guayabo sometidas a las mismas etapas del primer experimento, aplicadas en las concentraciones de 0,5; 1; 5; 10; 25; 50 y 75 g del sustrato por litro de agua. Trampas cebadas con agua de desagüe manifiestan mayor atractividad para la ovoposición del A. aegypti y el extracto de la hoja del guayabo presenta mayor porcentaje de atractividad de huevos del mosquito vector. Estos resultados pueden ser útiles para orientar la adopción de métodos de vigilancia entomológica con ovotrampas y control del A. aegypti.

RESUMO: Esta nota é o primeiro registro de Syntomopus parisii De Santis 1976 e Leptomeraporus sp. (Hymenoptera, Pteromalidae) parasitando simultaneamente Melanagromyza sojae Zehntner, 1900 (Diptera, Agromyzidae) no Brasil. Os parasitoides Pteromalidae são inimigos naturais de moscas minadoras do caule, abrindo perspectivas para o controle biológico da mosca minadora do caule da soja.

ABSTRACT: This note is the first report of Syntomopus parisii De Santis 1976 and Leptomeraporus sp. (Hymenoptera, Pteromalidae) simultaneously parasitizing Melanagromyza sojae Zehntner, 1900 (Diptera, Agromyzidae) in Brazil. The Pteromalidae parasitoids are natural enemies of stem miner flies, opening perspectives for biological control of soybean stem miner fly.

RESUMO: O uso do parasitoide Neodusmetia sangwani para controlar a cochonilha-das-pastagens Antonina graminis é um dos melhores exemplos de controle biológico clássico no mundo. A situação não poderia ser diferente no Brasil, onde a praga foi introduzida e mantida sob controle após a liberação de seu inimigo natural. Nessa revisão são reunidas informações sobre o bem-sucedido uso de um inimigo natural nesse país. Serão apresentadas também notas sobre a descrição e biologia dos insetos envolvidos e procedimentos de multiplicação e liberação do parasitoide para o controle dessa cochonilha no Brasil.

ABSTRACT: The use of the parasitoid Neodusmetia sangwani to control the Rhodes grass mealybug Antonina graminis is one of the best examples of classical biological control in the world. The situation could not be different in Brazil, where the pest has also been introduced, and kept under control after the release of its natural enemy. In this review, it is united information with respect to the successful use of a natural enemy in this country. Notes about the description and biology of the insects involved and rearing and release procedures of the parasitoid for the Rhodes grass mealybug control in Brazil will also be presented.

RESUMO: As cochonilhas-farinhentas, dentre elas Planococcus citriRisso, 1813 (Hemiptera: Pseudococcidae), são apontadas como responsáveis por ocasionar problemas fitossanitários na cultura da videira. Esta nota relata, pela primeira vez no Brasil, a ocorrência de um parasitoide no controle de P. citri. Para prospecção de inimigos naturais associados a P. citri, foram realizadas coletas de cachos de uvas infestados por essa cochonilha-farinhenta, semanalmente, durante o período de um mês, em áreas de produção de uvas finas de mesa, com infestação natural da praga, no município de Petrolina-PE. Coccidoxenoides perminutusGirault, 1915 (Hymenoptera: Encyrtidae), foi a única espécie de inimigo natural obtida. A partir desse registro, pretende-se estudar o seu potencial de parasitismo, bem como as estratégias de uso desse agente de controle natural.

ABSTRACT: From those of the mealybug family, the citrus mealybug, Planococcus citriRisso, 1813 (Hemiptera: Pseudococcidae) is frequently held responsible for various diseases occurring in vineyards. This is a first time report in Brazil, on the occurrence of a parasitoid controlling P. citri. In our search for the natural enemies of P. citri, bunches of grapes with the vine were collected every week for one month, in those regions where table grapes were produced, like Petrolina, Pernambuco, Brazil. In these samples, among the natural pest infestations Coccidoxenoides perminutusGirault, 1915 (Hymenoptera: Encyrtidae) was the only natural enemy species reported. In this research, we attempted to study the potential of parasitism as well as the strategies that this natural agent uses in pest control.

RESUMO As moscas-das-frutas (Diptera: Tephritidae), Anastrepha spp. e Ceratitis capitata(Wiedemann), são importantes pragas da fruticultura no Brasil. Para desenvolver um sistema sustentável de manejo integrado para este grupo de pragas, é fundamental conhecer os parasitoides (Hymenoptera) que podem regular as populações destes tefritídeos. Portanto, o objetivo deste estudo foi relatar a diversidade, a distribuição geográfica e as relações tritróficas dos himenópteros parasitoides de moscas-das-frutas, na região do Baixo Jaguaribe, no semiárido do Estado do Ceará, Brasil. Foram realizadas coletas de frutos em sete municípios da região, no período de maio de 2010 amaio de 2013. Os frutos foram levados para o laboratório, onde foram contados, pesados, colocados em bandejas plásticas com vermiculita e fechadas com tecido voile. Após sete dias, a vermiculita foi peneirada para a obtenção dos pupários das moscas-das-frutas que, em seguida, foram contados e acondicionados em placas de Petri, onde permaneceram até a emergência dos adultos (moscas e/ou parasitoides). Quatro espécies de parasitoides foram encontradas: Doryctobracon areolatus(Szépligeti), Opius bellus Gahan, Utetes anastrephae(Viereck) (Braconidae) e Tetrastichus giffardianusSilvestri (Eulophidae),sendo o mais frequente e com maior distribuição geográfica na região, D. areolatus. Doryctobracon areolatusfoi mais comum em associação com espécies de Anastrepha - A. sororcula Zucchi, A. obliqua (Mcquart) e A. zenildae Zucchi, em frutos nativos e com C. capitata em frutos exóticos. Tetrastichus giffardianus foi obtido apenas em associação com C. capitata, em frutos nativos e exóticos. Estas informações podem servir de base para inserção de parasitoides em futuros programas de manejo integrado de moscas-das-frutas, nas condições do Semiárido brasileiro.

ABSTRACT Fruit flies (Diptera: Tephritidae), Anastrepha spp. and Ceratitis capitata (Wiedemann), are important pests of Brazilian fruit-growing. In order to develop a system of pests integrated management in any region, it is essential to know the parasitoids (hymenoptera) which can regulate the populations of these tefritids. Therefore the objectives of this study were to know the diversity, geographic distribution and tritrophic relations of the hymenoptera parasitoids of fruit flies, in Baixo Jaguaribe region, located in Ceará semiarid, Brazil. Thus, fruits were collected in seven counties of the region, from May 2010 to May 2013. The fruits were taken to the laboratory, where they were counted, weighted, put in plastic trays with vermiculite and closed with voile. After seven or ten days, the vermiculite was sieved to obtain the puparium of the fruit flies. The puparium were counted and put in Petri dish, where they stayed until the emergence of the adults (flies and/or parasitoids). Four species of parasitoids were obtained: Doryctobracon areolatus (Szépligeti), Opius bellus Gahan, Utetes anastrephae (Viereck) (Braconidae) and (Eulophidae), Tetrastichus giffardianusSilvestri and the most frequently and with better geographical distribution in the region was D. areolatus. was Doryctobracon areolatus more common in association with species of Anastrepha - A. sororcula Zucchi, A. obliqua (Mcquart) and A. zenildaeZucchi, in native fruits and with C. capitata in exotic fruits. Tetrastichus giffardianus was obtained only in association with C. capitata in native and exotic fruits. This information can be used for inclusion of parasitoids in future integrated management programs of the fruit flies under the conditions of the Brazilian semiarid region.

This study reports seven species of whiteflies in the genera Aleurothrixus, Dialeurodicus, and Trialeurodes, as well as parasitism of Aleurothrixus floccosus by Signiphora sp. and Cales noacki. Three new hosts of Aleurothrixus aepim are reported. Dialeurodicus cockerellii and Trialeurodes vaporariorum are reported for the first time in Rio Grande do Sul State, Brazil.

En este trabajo se registran siete especies de moscas blancas, incluyendo los géneros Aleurothrixus, Dialeurodicus y Trialeurodes, y el parasitismo de Aleurothrixus floccosus por Signiphora sp. y Cales noacki. Tres nuevos huéspedes son reportados a Aleurothrixus aepim. Dialeurodicus cockerellii y Trialeurodes vaporariorum se registran por primera vez en Rio Grande do Sul, Brasil.

RESUMO O percevejo marrom, Euschistus heros (Hemiptera: Pentatomidae), é um inseto praga na cultura da soja no estado do Mato Grosso, Brasil. Nesta região, inseticidas sintéticos são frequentemente utilizados ao controle de insetos. Uma alternativa para o uso indiscriminado de inseticidas é o controle biológico com parasitoides. Assim, o objetivo deste estudo foi realizar o levantamento dos parasitoides que utilizam adultos de E. heros como hospedeiros. Para isso, amostras aleatórias foram conduzidas durante as safras de 2009/10 e 2010/11, em duas fazendas produtoras de soja (sistema convencional) em Tangará da Serra, Mato Grosso, Brasil. O número total de E. heros coletados foi: 297 (Área 1) e 293 (Área 2) em 2009/10 e 295 (Área 1) e 376 (Área 2) em 2010/11. Destes percevejos 1,50 (Área 1) e 13,99% (Área 2) encontravam-se parasitados em 2009/10 e 8,47 (Área 1) e 7,45% (Área 2) em 2010/11. Nas duas áreas, o parasitoide encontrado foi Hexacladia smithii Ashmead (Hymenoptera: Encyrtidae). Este é o primeiro registro de parasitismo em adultos de E. heros no estado do Mato Grosso, Brasil.

The neotropical stink brown bug, Euschistus heros (F.) (Hemiptera: Pentatomidae), is an insect pest to soybean crops in Mato Grosso State, Brazil. In this region, synthetic insecticides are frequently used for insect control. An alternative to the indiscriminate use of insecticides is the biological control with parasitoids. Thus, the objective of this study was to conduct the survey of parasitoids that use E. heros adults as hosts. Random samples were conducted during the harvests of 2009/10 and 2010/11 in two farms that produce soybean (conventional system) in Tangará da Serra, Mato Grosso State, Brazil. The total number of collected E. heros was: 297 (Field 1) and 293 (Field 2) in 2009/10 and 295 (Field 1) and 376 (Field 2) in 2010/11. Of these, 1.50 (Field 1) and 13.99% (Field 2) were parasitized in 2009/10 and 8.47 (Field 1) and 7.45% (Field 2) in 2010/11. The parasitoids found were Hexacladia smithii Ashmead (Hymenoptera: Encyrtidae) in both fields. This is the first record of parasitism in E. heros adults in the state of Mato Grosso, Brazil.

Parasitismo em ninfas de Bemisia tabaci (Genn.) biótipo B em folhas de algodão foi observado durante pesquisa sobre resistência de genótipos de algodoeiro a essa mosca-branca. O experimento foi conduzido em casa de vegetação no Centro Experimental do Instituto Agronômico de Campinas (IAC). Amostras das ninfas parasitadas foram mantidas em laboratório para acompanhamento do parasitismo e obtenção dos adultos dos parasitoides. Foram coletados 129 adultos, sendo um espécime de Encarsia inaron (Walker), 13 de En. lutea (Masi) e 115 de Eretmocerus mundus Mercet (Hymenoptera: Aphelinidae). Este é o primeiro registro de Er. mundus no Brasil.

The parasitism of Bemisia tabaci (Genn.) biotype B nymphs on cotton plants was observed during a research on resistance of cotton genotypes to this whitefly. The experiment was set in a greenhouse at the Experimental Station of the Instituto Agronômico (IAC), in Campinas, São Paulo State, Brazil. Samples of the parasitized nymphs were collected and maintained in laboratory to monitor the parasitism and obtain the adult parasitoids. A total of 129 adult parasitoids were obtained, including one Encarsia inaron (Walker), 13 En. lutea (Masi), and 115 Eretmocerus mundus Mercet (Hymenoptera: Aphelinidae). This is the first report of Er. mundus in Brazil.