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ABSTRACT Copper is an essential element for beet; however, it needs to be made available in adequate quantities since this nutrient, which is a heavy metal, can cause toxicity to plants and/or humans. This study aimed to evaluate the agronomic performance of beet fertigated with copper in a semi-arid region of Brazil. Two experiments were carried out in complete randomized blocks, with five treatments (0, 1.5, 3.0, 4.0, and 6.0 kg ha-1 of copper) and four replicates. In 2019, the copper content was within the adequate range for beet. In 2021, fertigation with copper above 0.8 kg ha-1 led to contents in the phytotoxicity range, which may explain the reduction in total and tuberous root dry mass accumulation. Fertilization with copper increased marketable yield in 2019 (17.32 t ha-1 at the dose of 3.9 kg ha-1 of copper), while, in 2021, there was no fit of the equations. Copper fertilization altered the tuberous root pH but did not influence the other tuberous root quality components. Plants fertilized with copper accumulated more copper in the leaves than in the tuberous root, with a maximum accumulation of 6.0 kg ha-1 of copper in 2019 and 2021. Fertilization with 3.9 kg ha-1 suits soils with low copper content.

RESUMO O cobre é um elemento essencial à beterraba, no entanto, é necessário disponibilizá-lo em quantidade adequada uma vez que esse nutriente, que é um metal pesado, pode causar toxicidade às plantas e, ou, ao ser humano. Esta pesquisa teve como objetivo avaliar o desempenho agronômico de beterraba fertirrigada com cobre em uma região semiárida do Brasil. Foram conduzidos dois experimentos em delineados em blocos casualizados ao acaso, com cinco tratamentos (0, 1.5, 3.0, 4.5 e 6.0 kg ha-1 de cobre) e quatro repetições. Em 2019, o teor de cobre estava dentro da faixa adequada para a beterraba. Em 2021, a fertirrigação com cobre acima de 0.8 kg ha-1 proporcionou teores na faixa de fitotoxicidade, o que pode explicar a redução no acúmulo de massa seca total e de raízes tuberosas. A adubação com cobre aumentou a produtividade comercial em 2019 (17.32 t ha-1 na dose de 3.9 kg ha-1 de cobre) enquanto que, em 2021, não houve modelo ajustado. A adubação alterou o pH da raiz tuberosa, mas não influenciou nos demais atributos de qualidade pós-colheita da raiz tuberosa. Plantas adubadas com cobre acumularam mais cobre nas folhas do que na raiz tuberosa, com máxima acumulada em 2019 e em 2021, na dose 6.0 kg ha-1 de cobre. A adubação com 3.9 kg ha-1 é adequada para solos com baixo teor de cobre.

https://doi.org/10.1590/1807-1929/agriambi.v28n3e276908

Physiological responses of tambaqui (Colossoma macropomum) fed diets supplemented with silage from fish and vegetables residues
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Costa, M. N. F.

; Furtado, Y. I. C.

; Monteiro, C. C.

; Brasiliense, A. R. P.

; Yoshioka, E. T. O

Brazilian Journal of Biology

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2024, Volume 84 elocation e255493

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A procura por insumos que substituam produtos de alto custo, como óleo e farinha de peixe, na fabricação de rações para uso na aquicultura é crescente, sendo necessário garantir a qualidade nutricional das dietas. A silagem produzida a partir de resíduos de pescado e de vegetais apresenta-se como uma alternativa de baixo custo e eficiente fonte proteica. O objetivo do presente estudo foi avaliar as respostas fisiológicas e bioquímicas de alevinos de tambaqui alimentados com quatro níveis de inclusão de silagem em ração comercial com 28% de proteína bruta, em dois períodos: 45 e 90 dias. Cada tratamento foi realizado em três repetições, com 10 tambaquis em cada caixa experimental de 100 L. Ao término de cada período estabelecido, amostras sanguíneas foram coletadas de cinco animais de cada repetição para determinação das variáveis hematológicas e bioquímicas. Peso, comprimento total e índices hepatossomático e lipossomático de espécimes alimentados com silagem não mostraram alterações significativas em ambos os períodos de avaliação, bem como os valores de hematócrito. A concentração de hemoglobina de tambaqui após 45 dias de alimentação aumentou quando foi fornecida dieta com inclusão de 20% de silagem. A contagem de eritrócitos, volume corpuscular médio e hemoglobina corpuscular média não apresentaram alterações entre os tratamentos, em ambos os períodos. As proteínas totais aumentaram significativamente no plasma de tambaquis que receberam dietas com inclusão de 5 e 10% de silagem, avaliados após 45 dias de alimentação. Evidenciou-se que os grupos com inclusão de silagem na dieta não apresentaram alterações significativas nos parâmetros avaliados, assim não sendo prejudiciais à higidez do tambaqui. Portanto, o uso da silagem como suplemento alimentar durante o cultivo do tambaqui é uma alternativa sustentável para produtores, por promover a redução do descarte de resíduos de pescado e da agricultura.

Abstract The demand for products to replace high-cost raw materials, such oil and fish meal, in the manufacture of feed for use in aquaculture, while also guaranteeing the nutritional quality of the diets, is increasing. Silage produced with fish and vegetables residues is a low-cost and efficient protein source. The objective of the present study was to evaluate the physiological and biochemical responses of tambaqui fingerlings fed four different levels of silage included in commercial feed with 28% crude protein, over two periods: 45 and 90 days. Each treatment was carried out over three replications, with 10 tambaqui in each 100 L experimental tank. At the end of each established period, blood samples were collected from five animals from each repetition to determine the hematological and biochemical variables. Body weight and total length, hepatosomatic and liposomal indices and hematocrit of specimens fed with diets supplemented with silage did not exhibit significant changes in both assessment period. After 45 days of feeding, the hemoglobin concentration increased when tambaqui were fed a diet including 20% silage. The red blood cell count, mean corpuscular volume and mean corpuscular hemoglobin did not change between treatments in either period. The total protein concentrations increased significantly in the plasma of tambaqui fed with diets with the inclusion of 5 and 10% of silage, evaluated after feeding for 45 days. It was found that the groups which had silage included in their diet did not exhibit significant alterations in the evaluated parameters, and the diet was therefore not consider harmful to the health of tambaqui. Therefore, the use of silage as a feed supplement during tambaqui farming is a sustainable alternative for producers, as it leads to a reduction of impacts of fish and vegetables waste disposal.

https://doi.org/10.1590/1519-6984.255493

Quality of life of women from a quilombola community in northeastern Brazil
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Santos, E. N. A.

; Magalhães, P. K. A.

; Santos, A. M.

; Correia, M. S.

; Santos, J. C. S.

; Carvalho Neto, A. P. M.

; Souza, M. A.

; Lima, R. F.

; Fonseca, S. A.

; Ferreira-Júnior, G. C.

; Cavalcanti, M. G. S.

; Costa, J. G.

; Matos-Rocha, T. J.

Brazilian Journal of Biology

Journal Metrics
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2024, Volume 84 elocation e246463

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo As comunidades quilombolas, estão presentes em diversos estados brasileiros, vivendo em condições de saúde mais precárias. Isto ocorre por conta do isolamento geográfico, das limitações de acesso e da falta de qualidade no serviço quando este é prestado. Nesse sentido, o objetivo do estudo foi analisar a qualidade de vida de mulheres de uma comunidade quilombola do nordeste brasileiro. Estudo observacional, transversal e descritivo. Foram entrevistadas 160 mulheres adultas, através de um formulário para a coleta de perfil e do questionário de Qualidade de Vida WHOQOL – bref. Foi observado que as mulheres tinham em média 40,7 anos (±17,25), casadas, autodeclaradas negras, com fundamental incompleto, do lar, sem renda, com moradia de alvenaria, própria, com até 6 cômodos, abastecidas por caixa de água comunitária, tratada. A Qualidade de Vida, apresentou escores medianos nos domínios: físico (3,18), psicológico (3,4), relações sociais (3,45) e meio ambiente (2,59). Com a realização desta pesquisa foi possível caracterizar a comunidade quilombola de Santa Luzia do Norte-AL quanto as dificuldades de acesso a saúde e geração de renda, fatos que repercutem na sua condição de saúde. Os problemas descritos neste estudo podem contribuir para que ações de saúde sejam planejadas e efetivadas com o intuito de melhorar as condições socioeconômicas e de saúde nessa comunidade, considerando-se o contexto social, político e ambiental, valorizando seus saberes e práticas tradicionais.

Abstract Quilombola communities are present in many Brazilian states living in precarious health conditions. This is due to geographic isolation, limitations to the access of the area in which they live in, and the lack of quality in the service when it is needed to be provided. Therefore, the aim of this study was to analyze the quality of life of women from a quilombola community in northeastern Brazil. It is an observational, cross-sectional and descriptive study. 160 adult women were first interviewed through a form to collect a profile and then it was applied the WHOQOL Quality of Life questionnaire – bref. It was observed that the women were on average 40.7 years old (±17.25), married, self-declared black, who did not finish elementary school, housewife, had no income, with their own masonry house, with up to 6 rooms, supplied by a box of community treated water. Quality of Life had median scores in the domains: physical (3.18), psychological (3.4), social relationships (3.45) and environment (2.59). With this research, it was possible to characterize the quilombola community of Santa Luzia do Norte-AL regarding the difficulties of access to health and income generation, issues that affect their health condition. The problems described in this study can contribute to health actions being planned and carried out in order to improve socioeconomic and health conditions in this community, considering the social, political and environmental context, valuing their traditional knowledge and practices.

https://doi.org/10.1590/1519-6984.246463

Quercetin inhibits neutrophil extracellular traps release and their cytotoxic effects on A549 cells, as well the release and enzymatic activity of elastase and myeloperoxidase
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Pereira, G. S.

; Percebom, I.

; Mendes, S.

; Souza, P. S. S.

; Diniz, L. F. A.

; Costa, M. F.

; Lopes, B. R. P.

; Toledo, K. A.

Brazilian Journal of Biology

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2024, Volume 84 elocation e252936

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo As armadilhas extracelulares de neutrófilos (NETs) foram relatadas pela primeira vez como uma estratégia microbicida para neutrófilos ativados. Por meio de uma resposta imunológica contra vários estímulos, os neutrófilos liberam seu DNA ligado a proteínas de grânulos, núcleo e citoplasma (por exemplo, elastase e mieloperoxidase). Até o momento, os NETs têm sido implicadas em danos aos tecidos durante processos inflamatórios intensos, principalmente quando sua liberação depende da geração de radicais de oxigênio. Os flavonóides são agentes antioxidantes e anti-inflamatórios, e destes, a quercetina é comumente encontrada em nossa dieta diária. Portanto, a quercetina pode exercer alguma atividade protetora contra o dano tecidual induzido por NETs. Em nossos ensaios in vitro, a quercetina reduziu a liberação das NETs, mieloperoxidase (MPO) e elastase a partir de neutrófilos estimulados com forbol 12-miristato 13-acetato (PMA). A atividade dessas enzimas também foi diminuída na presença de quercetina. A quercetina também reduziu o efeito citotóxico dos NETs sobre células alveolares (linha celular A549). Além disso, os ensaios in silico indicaram interações favoráveis entre a quercetina e as proteínas da NET (MPO e elastase). No geral, nossos resultados demonstram que a quercetina diminui os efeitos deletérios das NETs, reduzindo sua liberação à partir de neutrófilos ativados e diminuindo a atividade enzimática de MPO e elastase, possivelmente por meio de interação direta.

Abstract Neutrophil extracellular traps (NETs) were first reported as a microbicidal strategy for activated neutrophils. Through an immunologic response against several stimuli, neutrophils release their DNA together with proteins from granules, nucleus, and cytoplasm (e.g., elastase and myeloperoxidase). To date, NETs have been implicated in tissue damage during intense inflammatory processes, mainly when their release is dependent on oxygen radical generation. Flavonoids are antioxidant and anti-inflammatory agents; of these, quercetin is commonly found in our daily diet. Therefore, quercetin could exert some protective activity against tissue damage induced by NETs. In our in vitro assays, quercetin reduced NETs, myeloperoxidase (MPO), and elastase release from neutrophils stimulated with phorbol 12-myristate 13-acetate (PMA). The activity of these enzymes also decreased in the presence of quercetin. Quercetin also reduced the cytotoxic effect of NETs on alveolar cells (A549 cell line). Further, in silico assays indicated favorable interactions between quercetin and NET proteins (MPO and elastase). Overall, our results demonstrate that quercetin decreases deleterious cellular effects of NETs by reducing their release from activated neutrophils, and diminishing the enzymatic activity of MPO and elastase, possibly through direct interaction.

https://doi.org/10.1590/1519-6984.252936

Dopamine depletion in wistar rats with epilepsy
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Trindade-Filho, E. M.

; Pai, J. Dal

; Castro, D. N. de

; Silva, A. T. M. da

; Costa, A. F.

; Vieira, J. S. S.

; Santos, S. D. B. dos

; Félix, V. B.

; Leão, S. A. B. F.

; Zambrano, L. I.

; Saldanha-Filho, A. J. M.

; Carvalho, E. G. A.

; Cavalcante, J. B. N.

; Quintella, G. B.

; Lino, A. T. S.

; Costa, M. V.

; Lima, J. A.

; Tavares, M. M. A.

; de Melo, M. R.

; Mousinho, K. C.

; Biase, C. L. C. L. De

; Leite, M. L.

; Costa, P. J. M. S.

; Becker, E. L.

; Moura, I. M. F. B.

; Silva, J. C. da

Brazilian Journal of Biology

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2024, Volume 84 elocation e248411

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O conteúdo de dopamina nas estruturas cerebrais tem sido relacionado à excitabilidade neuronal e várias abordagens têm sido utilizadas para estudar este fenômeno durante o período de vulnerabilidade às crises. No presente trabalho, descrevemos os efeitos da depleção de dopamina após a administração de 6-hidroxidopamina (6-OHDA) na região pars compacta da substância negra de ratos submetidos ao modelo de epilepsia com pilocarpina. A susceptibilidade ao estado de mal epiléptico induzido pela pilocarpina, bem como a frequência de crises espontâneas e recorrentes durante o período crônico do modelo foi determinada. Sendo o hipocampo uma das principais estruturas afetadas no desenvolvimento desse modelo experimental de epilepsia, os níveis de dopamina nessa região foram determinados após a administração da droga. No primeiro experimento, 62% (15/24) dos ratos pré-tratados com 6-OHDA e 45% (11/24) daqueles que receberam ácido ascórbico como solução controle evoluíram para crises límbicas motoras e para o estado de mal epiléptico, após a administração de pilocarpina. A gravidade das crises durante o período agudo do modelo foi significativamente maior nos ratos epilépticos experimentais (56,52%) do que nos ratos controle (4,16%). No segundo experimento, não houve diferença significante entre os grupos quanto à frequência de crises na fase crônica do modelo. Nossos dados mostraram que a dopamina pode desempenhar um papel importante na gravidade das crises na fase aguda da pilo, o que parece ser exercido por sua ação inibitória da dopamina sobre a expressão motora das crises.

Abstract The dopamine content in cerebral structures has been related to neuronal excitability and several approaches have been used to study this phenomenon during seizure vulnerability period. In the present work, we describe the effects of dopamine depletion after the administration of 6-hidroxidopamine (6-OHDA) into the substantia nigra pars compacta of male rats submitted to the pilocarpine model of epilepsy. Susceptibility to pilocarpine-induced status epilepticus (SE), as well as spontaneous and recurrent seizures (SRSs) frequency during the chronic period of the model were determined. Since the hippocampus is one of main structures in the development of this experimental model of epilepsy, the dopamine levels in this region were also determined after drug administration. In the first experiment, 62% (15/24) of 6-OHDA pre-treated rats and 45% (11/24) of those receiving ascorbic acid as control solution progressed to motor limbic seizures evolving to SE, after the administration of pilocarpine. Severeness of seizures during the model´s the acute period, was significantly higher in epileptic experimental rats (56.52%), than in controls (4.16%). In the second experiment, the frequency of seizures in the model’s chronic phase did not significantly change between groups. Our data show that dopamine may play an important role on seizure severity in the pilo’s model acute period, which seems to be due to dopamine inhibitory action on motor expression of seizure.

https://doi.org/10.1590/1519-6984.248411

Evaluation of physicochemical properties of Nile tilapia skin collagen extracted in acid médium
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Costa, F. T.

; Oliveira, T. P.

; Droval, A. A.

; Marques, L. L. M.

; Fuchs, R. H. B.

; Cardoso, F. A. R.

Brazilian Journal of Biology

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2024, Volume 84 elocation e255440

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A tilápia tem tolerância a altas temperaturas, pode se reproduzir em cativeiro, crescer rápido e tem excelente custo-benefício. Por essas características, esta espécie é de grande interesse na aquicultura e, atualmente, o pescado mais produzido no Brasil. No entanto, com o aumento da produção de tilápias, houve também um aumento na quantidade de resíduos orgânicos, principalmente da filetagem, que descarta 70% dos resíduos. Existem muitos estudos sobre a extração de colágeno da pele de tilápia como alternativa para reduzir esses resíduos e agregar valor comercial. Neste trabalho, a extração do concentrado protéico foi testada utilizando um protocolo ácido, no qual as peles de tilápia foram submetidas a um pré-tratamento em meio ácido e precipitação salina, com variações de tempo e concentração. Após sua extração, a pele foi avaliada quanto a cinzas, umidade, proteína, solubilidade e pH. O concentrado protéico obtido apresentou baixos teores de cinzas, e a umidade está dentro dos apresentados pela literatura. O concentrado protéico apresentou teores de 68,73 a 80,58% de proteína e uma baixa solubilidade entre 4,03 a 6,93%. Em conclusão, a extração ácida é um possível meio de extração de colágeno, e a pele de tilápia é uma boa alternativa para reaproveitar os resíduos gerados na indústria de pescados.

Abstract Tilapia has high-temperature tolerance, can breed in captivity, grow fast, and have excellent cost-benefit. Because of these characteristics, this species is of great interest in aquaculture and, currently, the most produced fish in Brazil. However, by increasing tilapia production, there was also a rise in the amount of organic waste, mainly from filleting, which discards 70% of waste. There are many studies on collagen extraction from tilapia skin as an alternative to reduce these residues and add commercial value. In this work, the extraction of protein concentrate was tested using an acid protocol, in which the tilapia skins underwent a pre-treatment in an acid medium and saline precipitation, with variations in time and concentration. After its extraction, the skin was evaluated for ash, moisture, protein, solubility, and pH. The protein concentrate obtained showed low ash contents, and the humidity is within those presented by the literature. The protein concentrate showed levels from 68.73 to 80.58% of protein and a low solubility between 4.03 to 6.93%. In conclusion, acid extraction is a possible means of collagen extraction, and tilapia skin is a good alternative to reuse waste generated in the fish industry.

https://doi.org/10.1590/1519-6984.255440

Spatio-temporal analysis of the visceral leishmaniasis in the state of Alagoas, Brazil
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Ferreira, J. R. S.

; Silva, K. M.

; Cavalcanti, M. G. S.

; Ferreira-Júnior, G. C.

; Souza, E. C.

; Magalhães, P. K. A.

; Gomes, D. C. S.

; Fonseca, S. A.

; Maranhão, T. L. G. Q.

; Rocha, M. A. N.

; Nascimento, C. M. A.

; Lima, R. F.

; Alves, L. C.

; Brayner, F. A.

; Costa, J. G. da

; Matos-Rocha, T. J.

Brazilian Journal of Biology

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2024, Volume 84 elocation e253098

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A leishmaniose visceral é uma doença tropical negligenciada. Foram avaliadas a distribuição espacial dos casos de leishmaniose visceral no estado de Alagoas. Todos os casos de LV, registrados pela secretaria de saúde, foram analisados e georreferenciados. Entre 2008 e 2017, 97,1% dos municípios apresentaram classificação esporádica de transmissão. Com a evolução temporal, a incidência de casos de leishmaniose visceral se concentrou na maioria dos municípios da microrregião de Santana do Ipanema-AL. A análise espaço-tempo, se considerada, pode promover o aprimoramento das ações de vigilância e controle da leishmaniose visceral.

Abstract Visceral leishmaniosis is a neglected tropical disease. We evaluated the spatial distribution of cases of visceral leishmaniosis in the state of Alagoas, Brazil. All cases of VL, registered by the health department, were analyzed and georeferenced. Results: Between 2008 and 2017, 97.1% of the municipalities presented sporadic classification of transmission. With temporal evolution, the incidence of cases of visceral leishmaniosis was concentrated in most municipalities in the microregion of Santana do Ipanema-AL. Space-time analysis, if considered, may promote the improvement of surveillance and control actions of visceral leishmaniosis.

https://doi.org/10.1590/1519-6984.253098

Non-destructive method for estimating chrysanthemum leaf area
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Silva, Toshik I. da

; Ribeiro, João E. da S.

; Dias, Marlon G.

; Cruz, Renata R. P.

; Macêdo, Larissa F.

; Nóbrega, Jackson S.

; Sales, Giuliana N. B.

; Santos, Erli P. dos

; Costa, Franciscleudo B. da

; Grossi, José A. S.

Revista Brasileira de Engenharia Agrícola e Ambiental

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dec 2023, Volume 27 N. 12 Pages 934 - 940

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Chrysanthemum (Dendranthema grandiflora) is the second most produced and commercialized ornamental plant in the world. Measuring leaf area through non-destructive methods is fundamental for studies on its growth and production. The estimation of leaf area by linear dimensions of the leaves can be a strategy for this purpose. The objective of this study was to find allometric equations to estimate the leaf area of chrysanthemum. The linear, linear without intercept, quadratic, cubic, power, and exponential regression models were used for the analysis. The choice of equations was based on the highest coefficients of determination. The non-destructive method using allometric models has accuracy for estimating the leaf area (LA) of chrysanthemum from the product between leaf length (L) and leaf width (W). The LA of chrysanthemum can be estimated using the equation ŷ = 0.6611*LW0.9490 (L - leaf length; W - leaf width). This equation will allow researchers and producers to determine leaf area non-destructively.

RESUMO O crisântemo (Dendranthema grandiflora) é a segunda planta ornamental mais produzida e comercializada no mundo. A medição da área foliar por métodos não destrutivos é fundamental para estudos sobre seu crescimento e produção. A estimativa da área foliar por dimensões lineares das folhas pode ser uma estratégia para este fim. O objetivo deste estudo foi encontrar equações alométricas para estimar a área foliar do crisântemo. Os modelos de regressão linear, linear sem intercepto, quadrático, cúbico, potência e exponencial foram utilizados para a análise. A escolha das equações foi baseada nos maiores coeficientes de determinação. O método não destrutivo por meio de modelos alométricos tem acurácia para estimar a área foliar (AF) do crisântemo a partir do produto entre o comprimento da folha (C) e a largura da folha (L). A AF do crisântemo pode ser estimada pela equação ŷ = 0,6611*CL0,9490 (C - comprimento da folha; L - largura da folha). Essa equação permitirá que pesquisadores e produtores determinem a área foliar de forma não destrutiva.

https://doi.org/10.1590/1807-1929/agriambi.v27n12p934-940

Long-term oncological and surgical outcomes after Video Endoscopic Inguinal Lymphadenectomy (VEIL) in patients with penile cancer
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Tobias-Machado, Marcos

; Ornellas, Antonio A.

; Hidaka, Alexandre K.

; Medina, Luis G.

; Mattos, Pablo A. L.

; Besio, Ruben S.

; Abreu, Diego

; Castro, Pedro R.

; Nishimoto, Ricardo H.

; Astigueta, Juan

; Dourado, Aurus

; Machado, Roberto D.

; Magnabosco, Wesley J.

; Corona-Montes, Victor

; Villoldo, Gustavo M.

; Zampolli, Hamilton C.

; Taha, Anis

; Auad, Pericles R.

; Faria, Eliney F.

; Arantes, Paulo B. O.

; Tavares, Alessandro

; Nascimento, Francisco S. M. S.

; Brazão Jr., Eder S.

; Rocha, Maurício M.

; Costa, Walter H.

; Panico, Vinicius

; Reis, Leonardo O.

; Almeida-Carrera, Roberto J.

; Silva, Rafael C.

; Zequi, Stênio C.

; Calixto, José R. R.

; Sotelo, Rene

International braz j urol

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oct 2023, Volume 49 N. 5 Pages 580 - 589

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer.

https://doi.org/10.1590/s1677-5538.ibju.2023.0065

10.

Growth and yield of cauliflower with brackish waters under hydroponic conditions
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Silva, Mairton G. da

; Costa, Leandro F. da

; Soares, Tales M.

; Gheyi, Hans R.

Revista Brasileira de Engenharia Agrícola e Ambiental

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sep 2023, Volume 27 N. 9 Pages 663 - 672

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Historically, and in the aggravating climate change scenario, droughts are increasingly severe in arid and semi-arid regions, limiting the use of irrigation. As an alternative for these regions, brackish waters have been used, despite the severe risks of soil salinization, as well as losses in crop production and quality. Thus, the adoption of adequate technologies should mitigate or control the impacts caused by salt stress. Therefore, in the present study two experiments were conducted to evaluate the cultivation of cauliflower with brackish waters using a nutrient film technique (NFT) hydroponic system from July to October 2019 (winter-spring) and from October 2019 to January 2020 (spring-summer). Cauliflower plants were subjected to six values of ECw: 0.3 - control (without NaCl), 1.5, 2.5, 3.5, 4.5, and 5.5 dS m-1 (with NaCl), in a randomized block design with six replicates. For the leaf blade area at inflorescence harvest, reductions per dS m-1 increment in ECw of 7.22 and 6.41% were found in the winter-spring and spring-summer experiments, respectively. The quality losses of cauliflower inflorescences were more pronounced in the spring-summer experiment, varying according to the ECw used. Therefore, it is possible to grow cauliflower hydroponically under ECw of up to 5.5 dS m-1; however, in the hottest seasons these waters should be reserved and used only for the preparation of nutrient solutions or replacement of water consumed by plants.

RESUMO Historicamente e com o agravamento das mudanças climáticas, nas regiões áridas e semiáridas as secas são cada vez mais severas, limitando-se o uso da irrigação nas culturas. Como solução para essas regiões, as águas salobras têm sido utilizadas, mesmo sabendo-se dos graves riscos de salinização dos solos, bem como das perdas de produção e qualidade dos produtos colhidos. Assim, a adoção de tecnologias adequadas deve mitigar ou controlar os impactos causados pelo estresse salino. Portanto, no presente estudo foram realizados dois experimentos visando avaliar o cultivo da couve-flor com águas salobras usando o sistema hidropônico NFT (técnica do fluxo laminar de nutrientes) entre julho e outubro de 2019 (inverno-primavera) e outubro de 2019 e janeiro de 2020 (primavera-verão). As plantas foram submetidas a seis valores de CEa: 0,3 - controle (sem NaCl); 1,5; 2,5; 3,5; 4,5 e 5,5 dS m-1 (preparadas com NaCl), no delineamento em blocos casualizados com seis repetições. Na colheita das inflorescências foram encontradas reduções por dS m-1 de incremento na CEa de 7,22 e 6,41% para a área do limbo foliar nos experimentos de inverno-primavera e primavera-verão, respectivamente. As perdas de qualidade das inflorescências da couve-flor foram mais acentuadas no experimento da primavera-verão, variando de acordo com a CEa usada. Portanto, é possível o cultivo da couve-flor sob CEa de até 5,5 dS m-1; porém, nas estações mais quentes essas águas devem ser reservadas e usadas apenas para o preparo das soluções nutritivas ou para reposição do consumo hídrico das plantas.

https://doi.org/10.1590/1807-1929/agriambi.v27n9p663-672

11.

Dereplication of Sclerotiorin-Like Azaphilones Produced by Penicillium meliponae Using LC-MS/MS Analysis and Molecular Networking
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Peres, Eldrinei G.

; Souza, Mayane P.

; Sousa, Thiago F.

; Silva, Carlos V. A. da

; Barros, André L.

; Silva, Felipe M. A. da

; Costa, Emmanoel V.

; Medeiros, Lívia S. de

; Forim, Moacir R.

; Souza, Afonso D. L. de

; Paz, Weider H. P.

; Silva, Gilvan F. da

; Souza, Antonia Q. L. de

; Koolen, Hector H. F.

Journal of the Brazilian Chemical Society

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aug 2023, Volume 34 N. 8 Pages 1139 - 1153

Abstract: En | Text: En | PDF: En

Penicillium meliponae, a recently described and rare species, was isolated as an endophytic fungus from the Amazonian plant Duguetia sthelechantha, and has been proven to be a pigment producer. Considering the high productivity of this species and the lack of data on its chemical composition, the present study aimed to characterize the chemical profile of P. meliponae and evaluate the influence of agitation and the use of different culture media. For this purpose, liquid chromatography coupled with mass spectrometry (LC-MS/MS) and molecular networking were used, allowing the identification of 17 azaphilone molecules with sclerotiorin-like skeletons, becoming the first chemical report of this species. In addition, the different production patterns in the tested culture media were indicative that this species is sensitive to changes in the composition of the carbon source and to the presence of agitation. Furthermore, this work contributes to the fragmentation mechanisms of the different possible structural arrangements for azaphilones of the sclerotiorin type and serves as a repository of information on the gas-phase behavior of this type of metabolite in mass spectrometry experiments and will assist future studies aimed at the discovery of azaphilones.

https://doi.org/10.21577/0103-5053.20230027

12.

Hardened state and binder efficiency of concrete produced with wastes of porcelain polishing and scheelite
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Costa, C. Y. M. da

; Medeiros, A. G. de

; Codes, R. N. de

; Gurgel, M. T.

; Lima, F. J. N. de

Cerâmica

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jul 2023, Volume 69 N. 391 Pages 201 - 211

Abstract: En | Text: En | PDF: En

Abstract Reducing Portland cement consumption in concrete compositions, managing industrial waste, and conserving natural resources are contemporary challenges to achieving sustainable development. One solution has been the utilization of these industrial wastes as partial replacements for Portland cement or aggregates in concrete production. Due to the significant variability of these wastes, further research on this topic is necessary. Therefore, this study aimed to evaluate the properties of concrete mixtures containing porcelain polishing residue (PPR), scheelite residue (SR), and different types of Portland cement. The physical, chemical, and mineralogical characteristics of the wastes were determined. The fresh and hardened properties of the concretes, as well as the analysis of environmental impact, in terms of carbon dioxide emissions related to binder consumption, were investigated. The results showed that the utilization of these wastes resulted in reductions in consistency, water absorption, porosity, and compressive strength at 28 days by up to 33%, 16%, 13%, and 7%, respectively. Furthermore, improvements in microstructure, binder index, and CO2 index were observed. The wastes reduced the cement content and CO2 emissions per cubic meter of concrete to achieve 1 MPa by up to 12%. These findings suggested that the utilization of these wastes may be a viable and sustainable alternative to reduce Portland cement consumption and mitigate the environmental impact of the concrete industry. However, further research and studies are needed to assess other aspects, such as long-term durability and economic feasibility before their widespread adoption in the construction industry.

https://doi.org/10.1590/0366-69132023693913453

13.

Chemical attributes of an ultisol cultivated with sugarcane after application of high doses of vinasse
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Costa, Monalisa S.

; Rolim, Mário M.

; da Silva, Gerônimo F.

; Santos Júnior, José A.

; Pedrosa, Elvira M. R.

Revista Caatinga

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jun 2023, Volume 36 N. 2 Pages 402 - 413

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT The use of high doses of vinasse in sugarcane crop can cause modifications in the chemical characteristics of the soil. The aim of this study was to evaluate the effect of the application of high doses of vinasse on the chemical characteristics of an Ultisol cultivated with sugarcane for 210 days. The treatments used were doses equivalent to 0 (D0), 150 (D150), 300 (D300), 600 (D600) and 1200 (D1200) m3 ha-1 of vinasse plus a control treatment with mineral fertilization. The experimental design was completely randomized with six treatments and six repetitions. Exchangeable potassium (K), calcium (Ca), magnesium (Mg) and sodium (Na) contents, organic matter, cation exchange capacity, sodium adsorption ratio, exchangeable sodium percentage, electrical conductivity and pH were analyzed at 90, 120, 150 and 210 days after planting (DAP), in addition to microbial basal respiration at 210 days at depths of 0-0.20 and 0.20-0.40 m. Soil pH increased with the use of vinasse within the proper range for sugarcane, varying from 6.2 to 6.4. The highest K and Na contents were found at D600 and D1200; the highest K and Na availability occurred at 120 DAP and 150 DAP, respectively, indicating competition between these nutrients for exchange sites. Organic matter content was proportionally high according to the vinasse dose applied. D600 and D1200 doses increased Na content in the soil, with greater magnitude at the 0-0.20 m depth.

RESUMO O uso de doses elevadas de vinhaça no cultivo da cana-de-açúcar modifica os atributos químicos do solo. Objetivou-se com o presente trabalho avaliar o efeito da aplicação de doses elevadas de vinhaça sobre características químicas de um Argissolo Amarelo cultivado com cana-de-açúcar. Os tratamentos consistiram na aplicação de doses equivalentes a 0 (D0), 150 (D150), 300 (D300), 600 (D600) e 1200 (D1200) m3 ha-1 de vinhaça e mais uma testemunha. O delineamento experimental adotado foi inteiramente casualizado, com seis tratamentos e seis repetições. Avaliou-se a concentração de potássio (K), cálcio (Ca), magnésio (Mg) e sódio (Na) trocável, matéria orgânica, Capacidade de Troca Catiônica, Razão de Adsorção de Sódio, Porcentagem de Sódio Trocável, Condutividade Elétrica e pH aos 90, 120, 150 e 210 dias após o plantio (DAP), além da respiração basal microbiana aos 210 dias. O experimento foi conduzido em vasos com capacidade de 100 L. O pH do solo aumentou com a aplicação da vinhaça dentro da faixa considerada como adequada para a cana-de-açúcar, variando de 6,2 a 6,4. O K e Na apresentaram maiores concentrações em D600 e D1200; as maiores disponibilidades de K e Na no solo ocorreram, respectivamente, aos 120 e 150 DAP, indicando competição entre os elementos pelos sítios de troca. O teor de matéria orgânica foi proporcionalmente elevado conforme a dose de vinhaça aplicada. As doses D600 e D1200 aumentam a concentração de Na no solo, com maior magnitude na profundidade 0 – 0,20 m.

https://doi.org/10.1590/1983-21252023v36n217rc

14.

Production of Silver Nanoparticles Mediated by Aqueous Extracts of Tucumã (Astrocaryum aculeatum) Pulp
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Santos, Sidney S. dos

; Couto, Ricardo A. A. de

; Silva, Ingrid R. da

; Aouada, Marcia Regina M.

; Costantino, Vera R. L.

; Costa, Luiz P. da

; Perotti, Gustavo F.

Journal of the Brazilian Chemical Society

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may 2023, Volume 34 N. 5 Pages 705 - 712

Abstract: En | Text: En | PDF: En

Tucumã (Astrocaryum aculeatum) pulp presents a large number of secondary metabolites that can mediate the synthesis of metallic nanoparticles through a greener and more sustainable synthetic route than traditional ones. This study investigated the production of silver nanoparticles (AgNPs) mediated by aqueous extracts of tucumã pulp assessing different parameters, such as temperature, concentration of extract, and pH values of the reaction media. The alkaline reaction media produced the most intense and well-defined bands around 400 nm in the electronic spectra, indicating the formation of AgNPs in the three concentrations of tucumã pulp evaluated. The increase in the temperature of the reaction media also enhanced the surface plasmon resonance band of AgNPs but it increased the polydispersivity of produced nanoparticles according to the dynamic light scattering (DLS) and transmission electron microscopy (TEM) data. Zeta potential results indicated moderate stability of the AgNPs (close to -30 mV). Antimicrobial assessment of the colloidal metal particles indicated microbicide activity against the Gram-negative Escherichia coli bacterium.

https://doi.org/10.21577/0103-5053.20220141

15.

Thermal synthesis of NiMn2O4 spinel employing Mn oxide ores as starting material
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Nascimento, R. S.

; Figueira, B. A. M.

; F. Neto, E.

; Rabelo, A. A.

; Fernandez, O. J. C.

; Luz, P. T. S. da

; Costa, M. L. da

Cerâmica

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apr 2023, Volume 69 N. 390 Pages 154 - 160

Abstract: En | Text: En | PDF: En

Abstract It is described for the first time the synthesis and characterization of NiMn2O4 employing the Mn ores from the Amazon Region as starting material, instead of using commercial reagents as normally occurs in its synthesis process. Initially, an Mn ore was transformed into a single product, the octahedral layer (OL) manganese oxide with Ni2+ ions interlayer (Ni-OL-2), which was used as a matrix to obtain NiMn2O4 by thermal decomposition. The following techniques were used for characterization: X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), infrared spectroscopy (FTIR), thermal analysis (TG-DTA), scanning (SEM) and transmission (TEM) electron microscopies. The results revealed that Mn oxide ore composed of nsutite mineral transformed into a well-ordered crystalline Ni-OL-2 phase, after the hydrothermal and cation exchange process. The thermal decomposition of Ni-OL-2 produced the nickel manganite phase at 850 °C with spherical ball morphology and crystal sizes in the range of 200-300 nm.

https://doi.org/10.1590/0366-69132023693903436

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