Abstract Non-tuberculous mycobacteriosis, previously known as atypical, anonymous, opportunistic, or unclassified mycobacteriosis, refers to pathogenic mycobacterioses other than those caused by Mycobacterium tuberculosis and Mycobacterium leprae. These mycobacteria are known for their environmental distribution, mainly in water and soil. The incidence of non-tuberculous mycobacteriosis has been increasing in all countries and skin infections are being increasingly studied, mainly with the increase in immunosuppressive conditions and the development of new medications that affect immunological function. In the present article, a detailed narrative review of the literature is carried out to study the main non-tuberculous mycobacteriosis that cause diseases of the skin and appendages. The article also aims to present a historical context, followed by epidemiological, microbiological, and clinical characteristics of these diseases. Practical considerations about the diagnosis and treatment of non-tuberculous mycobacteriosis are detailed.

Abstract Microbiological safety of fish is a concern of consumers, industries and regulatory agencies worldwide. Among the pathogenic microorganisms, Salmonella spp. is one of the main agents of foodborne diseases and should be absent in animal products. Rapid and accurate identification of pathogens in the supply chain is important for both quality assurance and tracking infectious agents within the chain. In this context, this study aimed to evaluate the equivalence of two rapid detection tests, as alternative methods to the conventional Salmonella detection method, as well as to verify the microbiological quality parameters of two commercially important fish species in the Amazon biome. The plate count of aerobic bacteria ranged from 7.76 x 104 to 8.71 x 107 CFU.g-1 for mesophiles and 1.70 x 106 to 4.27 x 108 CFU.g-1 for psychrotrophic whereas the maximum for this group of microorganisms in fresh fish is 106 CFU.g-1. Regarding the Staphylococcus count, the two species presented variations between 1.35 x 104 to 1.51 x 105 CFU.g-1. This represents unsatisfactory conditions of handling, storage and conservation of fish species. The immunoenzymatic and molecular methods have been shown to be reliable, fast and effective in the detection of Salmonella and for its high index of agreement with the conventional detection method. We also emphasize the convenience of multiplex PCR application due to the high sensitivity, specificity, speed and accuracy of Salmonella detection.

RESUMO: O objetivo deste trabalho é descrever um caso de salmonelose em um peixe-boi-da-amazônia (Trichechus inunguis). O animal foi resgatado em um afluente do rio Capim, no município de Goianésia do Pará, Pará, Brasil, sendo mantido em uma piscina e morrendo aproximadamente cinco meses após o resgate. As principais alterações observadas na necropsia foram ceco e colón com hiperemia da serosa; edema da parede; mucosa espessada, com áreas ulceradas e revestida por uma fina camada de fibrina; e conteúdo líquido amarelado. Histologicamente havia tiflocolite fibrinonecrótica, multifocal, moderada, associada a intensa colonização bacteriana. Em fragmentos de ceco e cólon foi isolada Salmonella enterica subsp. enterica (rugosa). Este é o primeiro relato de salmonelose em peixe-boi-da-amazônia.

ABSTRACT: The objective of this study was to describe a case of salmonellosis in an Amazonian manatee (Trichechus inunguis). The animal was rescued from a tributary of the Capim River, in the municipality of Goianésia do Pará, Pará, Brazil, kept in a pool, and died approximately five months after being rescued. The main changes observed at necropsy were that the cecum and colon had serosal hyperemia, wall edema, thickened mucosa with ulcerated areas and covered by a thin layer of fibrin, and a yellowish liquid content. Histologically, there was moderate multifocal fibrinonecrotic typhlocolitis associated with intense bacterial colonization. Salmonella enterica subsp. enterica (rough) was isolated in cecum and colon fragments. This is the first report of salmonellosis in an Amazonian manatee.

Abstract The Equine Strangles, caused by Streptococcus equi subs. equi, is a contagious disease, causing high rates of morbidity been responsible for important economic losses. The M protein synthesized by S. equi plays an important role in the pathogenesis and is a promising candidate for a vaccine antigen. The innate immune system is responsible for the first immune response against microorganisms, this response is mediated by receptors that detect PAMPs and their activation trigger crucial modulation of the adaptative immune response. This work describes the immune response of S. equi subs. equi. recombinant SeM protein, using Escherichia coli BL21 (DE3) as an expression and delivery vaccine system. To characterize and to determine the vaccine efficacy, mice were vaccinated as followed: 1. Recombinant E. coli expressing rSeM protein; 2. The same recombinant E. coli, inactivated adsorbed in Alumen; 3. Purified rSeM protein adsorbed in Alumen; 4. Inactivated S. equi whole cells adsorbed in Alumen; 5. Control group. All vaccinated mice developed protective response against S. equi infection, however the groups that received the E. coli expressing rSeM presented significant higher IgG level than other vaccinated groups. The recombinant E. coli delivery vaccine system also induced a highest IgG response than inactivated S. equi or purified rSeM vaccines in horses. This study evidence that the recombinant E. coli, live or inactivated, enhanced the humoral response, reaching significant higher antibodies levels than those obtained in the vaccination with the bacterin or purified antigen, showing the feasibility of producing low-cost vaccines against strangles.

Abstract The role of women in the fisheries sector is largely underestimated and underreported. Although women are a fundamental part of the seafood supply chain in Brazil, fisheries management is gender-biased; sectoral programs and policies fail to recognize, support, and guarantee fisherwomen legal and labor rights. Brazilian fisherwomen have been very active in claiming their rights and recognition in the fisheries sector; however, public policies are lagging, and so are the studies that subsidize them. Within the UN Decade of Ocean Science for Sustainable Development and 2030 Agenda, it is critical to analyze the interactions between SDG 14 (Life below water) and SDG 5 (Gender equality) to discuss the gender dimensions underpinning fisheries (un)sustainability. We performed a systematic literature review and bibliometric analysis of gender-oriented studies in marine fisheries in Brazil. We identified 19 studies, published up to December 2020, focused on artisanal fisheries. The publications show that women are present in fisheries and are fundamental subjects to maintaining the activity and continuity of this livelihood. However, fisherwomen remain invisible. Their work is underreported, underpaid, and undervalued, which jeopardizes the sustainability of artisanal marine fisheries. Considering the knowledge gaps to be addressed during the Ocean Decade, we recommend that researchers and politicians work to: make "hidden workforce" of women visible, embrace interdisciplinarity, set research priorities, fill the data gap, and subsidize public policies. During the next few years, it is critical to enable and settle monitoring and assessment programs that provide open access to data, information, and technologies for the predictability, the sustainable harvesting of the ocean, and the correct design of gender-sensitive fisheries and aquaculture policies.

The objective of the present case report was to describe the aesthetic and functional rehabilitation of the upper anterior incisors of a patient with bruxism. A 23-year-old male patient, with bruxism, sought treatment for his upper anterior teeth due to diastemas and reduced lengths of the upper central incisors. The gingivoplasty and osteotomy were performed in the region of the upper central incisors to improve the positions of the gingival contours and increase the length of these teeth. After 90 days, teeth whitening was performed. After 1 month the composite resin veneers were made in the upper central incisors. During treatment, the needs and perspectives of the patient were considered to achieve clinical success. The treatments were finalized, and the final result was satisfactory. Therefore, an integrated treatment plan proved to be extremely important, especially when the patient is adequately informed of the available treatment options and collaborates with the treatment.

O objetivo do presente relato de caso clínico foi descrever a reabilitação estética e funcional dos incisivos anteriores superiores de um paciente com bruxismo. Paciente do gênero masculino, 23 anos de idade, bruxoma, procurou tratamento para os dentes anteriores superiores devido aos diastemas dentários e também por causa dos comprimentos reduzidos dos incisivos centrais superiores. Para isso, foi realizada a gengivoplastia e osteotomia na região dos incisivos centrais superiores com o objetivo de melhorar as posições dos contornos gengivais e aumentar o comprimento desses dentes. Após 90 dias, o clareamento dentário foi realizado. Depois de 1 mês as facetas de resina composta foram confeccionadas nos incisivos centrais superiores. Durante o tratamento as necessidades e perspectivas do paciente foram levadas em consideração para alcançar o sucesso clínico. Após a conclusão dos procedimentos, o resultado final foi satisfatório. Portanto, um plano de tratamento integrado se mostrou de extrema valia, principalmente quando o paciente é devidamente esclarecido das opções disponíveis de tratamento e colabora com o mesmo.

This experiment was conducted to study the substitution of soybean meal with peanut cake in the supplement by assessing the feeding behavior and the interaction between the thermal environment and the physiological responses of eight crossbred cows with an average live weight of 500 kg. Cows were divided into two 4 × 4 Latin squares and managed on pasture. The peanut cake was included at the levels of 0, 330, 660, and 1,000 g kg-1 in the supplement as a replacement for soybean meal. The feeding behavior variables were not affected by the levels of peanut cake. No differences were found for the physiological parameters of the animals with the replacement of soybean meal for peanut cake in the supplements. Peanut cake can replace up to 100% soybean meal in the supplement of grazing lactating crossbred cows without altering the feeding behavior or physiological parameters of the animals.

O artigo apresenta experiências desenvolvidas por museus no cenário contemporâneo, com o intuito de analisar as transformações no campo. Assim, apresenta brevemente possibilidades de ruptura com o modelo predominante de expor o passado como narrativa linear, ou mesmo revivê-lo por meio de encenações.

This paper presents the experiences developed by museums in the contemporary setting to analyze the transformations in this field. It thus briefly brings forth possibilities to break away from the prevailing model of exposing the past as a linear narrative or even of reliving it by means of enactments.

O artigo pretende abordar os desafios e as potencialidades do ensino de História nos museus, em um cenário de predomínio da memória como referência para narrativas e lutas políticas. Os materiais pedagógicos produzidos pelos museus serão um ponto de partida para perceber concepções de história, memória e aprendizagem instrumentalizadas pelos museus históricos com o objetivo de educar. O museu, concebido como espaço de poder, é produtor de sentidos do passado e, portanto, suas múltiplas linguagens fomentam acessos para a compreensão de permanências e rupturas dos projetos de História nacional. Neste sentido, a importância da preservação das fontes pedagógicas é realçada, como proposta para a construção de interpretações sobre a educação museal no Brasil.

This article analyzes the challenges and the potential of teaching history in museums, a scenario in which memory predominates as a reference for narratives and political confrontation. In the face of the growing demand of memory, museums are meaningful spaces of production and diffusion of the discourse on the past. Historical museums, regarded as spaces of power, afford pedagogical materials as starting points for the perception of the concepts of history, memory, and learning and lend meaning to the past. Their multiple languages lead to the understanding of the continuity and the discontinuity of the national history projects. The importance of preserving pedagogical sources is highlighted within the framework of museum education in Brazil.