Artificial lighting is one of the most powerful management tools available to commercial layer producers. Artificial light allows anticipating or delaying the beginning of lay, improving egg production, and optimizing feed efficiency. This study aimed at comparing the performance of commercial layers submitted to lighting using different LED colors or conventional incandescent lamps. The study was carried out in a layer house divided in isolated environments in order to prevent any influenced from the neighboring treatments. In total, 360 Isa Brown layers, with an initial age of 56 weeks, were used. The following light sources were used: blue LED, yellow LED, green LED, red LED, white LED, and 40W incandescent light. Birds in all treatment were submitted to a 17-h continuous lighting program, and were fed a corn and soybean meal-based diet. A completely randomized experimental design with subplots was applied, with 24 treatments (six light sources and four periods) of three replicates. Egg production (%) was significantly different (p<0.05) among treatments, with the best results obtained with red LED, white LED, and incandescent light sources. Egg weight, feed intake, and internal egg quality (albumen height, specific gravity, and Haugh units) were not influenced (p>0.05) by light source. It was concluded that the replacement of incandescent light bulbs by white and red LEDs does not cause any negative effect on the egg production of commercial layers.