Aim To evaluate the influence of cobalt-chromium (Co-Cr) coping fabrication methods and ceramic application on the marginal and internal fit of metal-ceramic crowns. Methods Co-Cr copings for metal-ceramic crowns were prepared by lost wax casting or CAD-CAM machining of sintered blocks. The fit was analyzed using the silicone replica technique at four assessment points: marginal gap (MG), axial wall (AW), axio-occlusal (AO) angle, and central occlusal (CO) wall. After the initial analysis, the copings were ceramic-veneered with the layering technique, and the fit was again determined. Data were statistically analyzed by paired and unpaired Student’s-t test (α=0.05). Results Marginal and internal fit before ceramic application according to the coping manufacturing method showed significant differences only at CO (p < 0.001), with milled copings (137.98±16.71 μm) showing higher gap values than cast copings (112.86±8.57 μm). For cast copings, there were significant differences at MG (before 109.13±8.79 μm; after 102.78±7.18 μm) and CO (before 112.86±8.57 μm; after 104.07±10.63 μm) when comparing the fit before and after ceramic firing. For milled copings, there was significant difference only at AO (before 116.39±9.64 μm; after 108.54±9.26 μm). Conclusion This study demonstrated that the coping fabrication method influenced the internal fit. Ceramic firing maintained or improved the fit of the metal-ceramic crowns. The marginal discrepancy of all restorations, before and after ceramic firing, can be considered clinically acceptable.

Resumo O objetivo deste estudo foi avaliar o efeito da sepse aguda no ligamento periodontal, osso alveolar e osso da furca por meio de análise histológica e imunohistoquímica. O modelo de sepse em ratos foi estabelecido pelo procedimento de ligação e perfuração do ceco (CLP). Doze ratos foram divididos de forma randomizada em ratos sépticos (n=6) e controle - grupo Sham (n=6). Os animais foram eutanasiados após 24 horas e suas hemimandíbulas foram submetidas aos procedimentos histotécnicos para análise histomorfométricos (matriz óssea, fibras colágenas, fibroblastos, osteócitos, células inflamatórias e vasos sanguíneos) e imunohistoquímicos (BMP-2/4, RANKL e osteocalcina) no osso alveolar, osso de furca e ligamento periodontal. Nossos resultados demonstraram que os parâmetros histomorfométricos foram similares no osso alveolar, osso de furca e ligamento periodontal dos animais do grupo sepse e do grupo Sham. Em relação à análise por imunohistoquímica, o número de células imunomarcadas para BMP-2/4 e RANKL também foi similar em ambos os grupos. Entretanto, houve redução (p=0.0014) no número de células imunomarcadas para osteocalcina no ligamento periodontal de ratos sépticos em relação ao grupo Sham. Como conclusão, o estabelecimento de sepse aguda resultou em um número reduzido de células imunomarcadas para osteocalcina na região do ligamento periodontal (p=0,0014). Além disso, não foram observadas diferenças histológicas significativas no periodonto de ratos na presença de sepse aguda. Considerando o papel da osteocalcina na remodelação óssea, este estudo contribui para revelar a importância da avaliação periodontal cuidadosa na presença de sepse.

Abstract The aim of this study was to evaluate the effect of acute sepsis in the periodontal ligament, alveolar and furcation bone in absence of periodontitis induction through histological and immunohistochemical analyses. A septic rat model was established by cecal ligation and puncture (CLP). Twelve rats were randomly divided into CLP (n=6) and Sham (n=6) groups. The animals were euthanized at 24 h and hemimandibles were submitted to histomorfometric (bone matrix, collagenous fibers, fibroblasts, osteocytes, inflammatory cells, and blood vessels) and immunohistochemical (BMP-2/4, RANKL and osteocalcin) evaluation in alveolar bone, furcation bone and periodontal ligament. Our results demonstrated that histomorphometric parameters were similar in alveolar bone, furcation bone and periodontal ligament of Sham and CLP rats. Regarding to immunohistochemical analyses, the number of BMP-2/4 and RANKL immunolabeled cells was also similar in both groups. Furthermore, it was detected a reduction in the osteocalcin immunolabeled cells in periodontal ligaments of CLP compared to Sham rats (p=0.0014). In conclusion, the acute sepsis induction resulted in reduced number of osteocalcin labelled cells in periodontal ligament region. Moreover, no significant histological differences were observed in the periodontium of rats under acute sepsis. Considering the role of osteocalcin in bone remodeling, the study contributes to revealing the importance of careful periodontal evaluation in the presence of sepsis.

With the objectives of dengue prevention, health promotion, and action-based research, two experiments were conducted in pilot areas of Buenos Aires and Vicente López, Argentina. In each area, community, government, and non-governmental references were identified. The study included an entomological survey (with ovitraps), environmental survey (with field observations, interviews, and workshops), and social survey (using questionnaires, interviews, and meetings). Spaces for dialogue and collaboration were developed with the community, thereby spawning participatory activities and empowerment. Environmental reordering and reduction of mosquito breeding sites were promoted by: incorporation of environmental issues and vector-borne disease prevention into the school curricula; neighborhood workshops; training of environmental agents to transmit information and train peers; and planning community environmental projects. The households were visited, seeking to integrate the residents into their own community environmental diagnosis. There were significant differences between the pilot areas in knowledge and social practices, but not according to socioeconomic status. The schools and health promotion settings proved to be the main community reference for promoting healthy environmental practices.

Con objetivos de prevención de dengue, promoción de la salud e investigación-acción se desarrollaron dos experiencias en zonas piloto de Buenos Aires y Vicente López. En cada zona se identificaron referentes comunitarios, gubernamentales y no gubernamentales. Se realizó un diagnóstico entomológico (mediante ovitrampas), ambiental (con observaciones en terreno, encuestas y talleres), y social (utilizando cuestionarios, entrevistas y reuniones). Se construyeron espacios de diálogo y trabajo conjunto con la comunidad, generando acciones participativas y empoderamiento. Se fomentó el ordenamiento ambiental y la reducción de criaderos de mosquitos a través de incorporación de temas ambientales y prevención de enfermedades vectoriales a los currícula escolares; talleres vecinales de difusión; capacitación de promotoras ambientales para transmitir información y para capacitar a pares; planificación de proyectos ambientales de la comunidad. Se entró en los domicilio, buscando integrar a sus habitantes en el diagnóstico ambiental propio y comunitario. Se registraron diferencias significativas entre conocimientos y prácticas sociales, para ambas zonas piloto, pero no por nivel socioeconómico. Las escuelas y los entornos de promoción de salud resultaron ser los principales referentes comunitarios para fomentar prácticas ambientales saludables.

With the objectives of dengue prevention, health promotion, and action-based research, two experiments were conducted in pilot areas of Buenos Aires and Vicente López, Argentina. In each area, community, government, and non-governmental references were identified. The study included an entomological survey (with ovitraps), environmental survey (with field observations, interviews, and workshops), and social survey (using questionnaires, interviews, and meetings). Spaces for dialogue and collaboration were developed with the community, thereby spawning participatory activities and empowerment. Environmental reordering and reduction of mosquito breeding sites were promoted by: incorporation of environmental issues and vector-borne disease prevention into the school curricula; neighborhood workshops; training of environmental agents to transmit information and train peers; and planning community environmental projects. The households were visited, seeking to integrate the residents into their own community environmental diagnosis. There were significant differences between the pilot areas in knowledge and social practices, but not according to socioeconomic status. The schools and health promotion settings proved to be the main community reference for promoting healthy environmental practices.

Con objetivos de prevención de dengue, promoción de la salud e investigación-acción se desarrollaron dos experiencias en zonas piloto de Buenos Aires y Vicente López. En cada zona se identificaron referentes comunitarios, gubernamentales y no gubernamentales. Se realizó un diagnóstico entomológico (mediante ovitrampas), ambiental (con observaciones en terreno, encuestas y talleres), y social (utilizando cuestionarios, entrevistas y reuniones). Se construyeron espacios de diálogo y trabajo conjunto con la comunidad, generando acciones participativas y empoderamiento. Se fomentó el ordenamiento ambiental y la reducción de criaderos de mosquitos a través de incorporación de temas ambientales y prevención de enfermedades vectoriales a los currícula escolares; talleres vecinales de difusión; capacitación de promotoras ambientales para transmitir información y para capacitar a pares; planificación de proyectos ambientales de la comunidad. Se entró en los domicilio, buscando integrar a sus habitantes en el diagnóstico ambiental propio y comunitario. Se registraron diferencias significativas entre conocimientos y prácticas sociales, para ambas zonas piloto, pero no por nivel socioeconómico. Las escuelas y los entornos de promoción de salud resultaron ser los principales referentes comunitarios para fomentar prácticas ambientales saludables.