Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with 60Co gamma rays, yielding toxoids with good immunogenicity. The achievement of modified antigens with lower toxicity and preserved or improved immunogenicity can be very useful. Ionizing radiation has already been proven to be a powerful tool to attenuate snake venom toxicity without affecting, and even increasing, their immunogenic properties. However, little is known about the modifications that irradiated molecules undergo and even less about the immunological response that such antigens elicit. In the present work, we investigated the immunological behavior of bothropstoxin-1, a K49 phospholipase, before and after irradiation. Structural modifications of the toxin were analyzed by SDS-PAGE. Isogenic mice were immunized with either the native or the irradiated toxin. The circulating antibodies were isotyped and titrated by ELISA. According to our data, irradiation promoted structural modifications in the toxin characterized by higher molecular weight forms of proteins (aggregates and oligomers). The results also indicated that irradiated toxins were immunogenic and antibodies elicited by them were able to recognize the native toxin in ELISA. These findings suggest that irradiation of toxic proteins can promote significant modifications in their structures; however they still retain many of the original antigenic and immunological properties of native proteins. Also, our data indicate that irradiated proteins induce higher titers of IgG2a and IgG2b, suggesting that Th1 cells are predominantly involved in the immune response.
A resposta de Acacia mangium Willd (mangium) e Sclerolobium paniculatum Vogel (tachi) à inoculação de fungos micorrízicos arbusculares (FMA), oriundos de áreas em recuperação após a extração de bauxita, foi avaliada em experimento com delineamento inteiramente casualizado, com 14 tratamentos (duas espécies leguminosas e sete tipos de solo) e três repetições. Avaliou-se o número de esporos no solo, a colonização micorrízica, a matéria seca total, o P acumulado, a dependência micorrízica das mudas, e a abundância e a freqüência de espécies. O número de propágulos infectivos (NPI) foi estudado em delineamento em blocos casualizados, com oito diluições de solo inóculo, cinco repetições e uma planta isca (Brachiaria decumbens Stapf). Utilizou-se substrato da mistura de um Planossolo mais areia lavada e fosfato de rocha araxá (0,60 g/kg). O número de esporos aumentou em função do tempo de cobertura das leguminosas. A colonização micorrízica foi mais intensa no tachi. Os valores de matéria seca dessa espécie foram inferiores aos de mangium, que por sua vez extraiu em torno de seis vezes mais P do substrato. Em geral, mangium, ao contrário do tachi, foi facultativa à presença dos FMA, sugerindo sua utilização na recuperação de áreas degradadas sem inoculação prévia. Dentre as 39 espécies de FMA identificadas, Glomus macrocarpum Tul. & Tul. apresentou maior índice de abundância e freqüência (IAF) e maior NPI, destacando-se entre as espécies pioneiras, ao passo que outras apareceram apenas em estádios sucessionais mais avançados das áreas em recuperação.
The responses of Acacia mangium Willd (mangium) and Sclerolobium paniculatum Vogel (tachi) to arbuscular mycorrhizal fungi (AMF) inoculation collected from areas under reclamation after bauxite mining, was evaluated in an completely randomized design distributed in 14 treatments (two legume, species and seven soil types), with three replicates. Evaluated were the number of spores in the soil, the mycorrhizal colonization, the total dry matter, accumulated P, the mycorrhizal dependence of the seedlings and the abundance and the frequency of species. The number of infective propagules (NIP) was studied in a randomized block design, with eight dilutions of soil inoculum, five replicates and a trap plant (Brachiaria decumbens Stapf). Substratum of a Planosol, mixed with washed sand (1:1 v:v), and phosphate of rock Araxá (0.60 g/kg) was used. The number of spores increased in function of time covering of legumes. The mycorrhizal colonization was more intense in tachi. The values of dry matter of that species were inferior to the mangium, that extracted six times larger P of substratum. In general, mangium, contrary to tachi, was facultative to presence of AMF, suggesting the use in degraded areas without previous inoculation. Among the 39 species of identified AMF, Glomus macrocarpum Tul. & Tul. showed higher index of abundance and frequency (IAF) and higher NIP, standing among pioneering species, while others appeared only under more developed forest of the areas in reclamation.