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Prevalence of syphilis and sexual behavior and practices among adolescents MSM and TrTGW in a Brazilian multi-center cohort for daily use of PrEP
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Westin, Mateus Rodrigues

; Martinez, Yuppiel Franmil

; Silva, Ana Paula

; Greco, Marília

; Marques, Lucas Miranda

; Campos, Guilherme Barreto

; Alves, Matheus de Paula

; Mancuzzo, Alessandra

; Tupinambás, Unaí

; Greco, Dirceu Bartolomeu

Cadernos de Saúde Pública

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2023, Volume 39 Suppl. 1 elocation e00118721

Abstract: Pt Es En | Text: Pt Es En | PDF: Pt | PDF: Es | PDF: En

A sífilis reemergiu enquanto problema sério de saúde pública no Brasil e no mundo, afetando de maneira desproporcional homens que fazem sexo com homens (HSH), travestis e mulheres transgênero. São relativamente escassos os estudos sobre infecções sexualmente transmissíveis (IST) entre adolescentes pertencentes a essas populações-chave. O estudo transversal multicêntrico brasileiro analisou a prevalência de sífilis na linha de base da coorte PrEP15-19 de HSH, travestis e mulheres transgênero adolescentes, sexualmente ativos, recrutados entre abril de 2019 e dezembro de 2020. As análises foram realizadas com as dimensões de vulnerabilidade a ISTs/HIV, e modelos de regressão logística foram construídos para estimar as razões de chances da associação entre as variáveis preditoras e um teste treponêmico positivo para sífilis na entrada do estudo. Foram analisados 677 participantes. A idade mediana era 18,9 anos (IIQ: 18,1-19,5), 70,5% (477) se autoidentificaram como pretos, 70,5% (474) como homossexuais/gays e 48 (7,1%) como mulheres trans ou travestis. A prevalência de sífilis na linha de base era 21,3%. No modelo final de regressão logística, as seguintes variáveis estiveram associadas com chances maiores de sífilis: autorrelato de episódio de IST nos últimos 12 meses (OR = 5,92; IC95%: 3,74-9,37), profissional do sexo (OR = 3,39; IC95%: 1,32-8,78) e menos de 11 anos de escolaridade (OR = 1,76; IC95%: 1,13-2,74). A prevalência de sífilis entre HSH, travestis e mulheres transgênero adolescentes entre 15 e 19 anos de idade foi alarmante, muito acima da taxa relatada na população geral nesta faixa etária, e esteve associada a fatores de vulnerabilidade. Os achados reforçam a necessidade urgente de fortalecer os programas de saúde pública com a discussão sobre raça, gênero, sexualidade e prevenção.

La sífilis ha resurgido como un problema serio de salud pública en Brasil y en todo el mundo, afectando desproporcionadamente a hombres que tienen sexo con hombres (HSH), travestis y mujeres transgénero (TrTGW). Los estudios de las enfermedades trasmitidas sexualmente (ETS) en adolescentes de estas poblaciones clase son relativamente escasos. Se trata de un estudio transversal brasileño multicéntrico, con análisis de prevalencia de la base de referencia de la cohorte PrEP1519 de hombres activos sexualmente que mantienen sexo con hombres y adolescentes TrTGW, reclutados desde abril de 2019 a diciembre de 2020. Se hicieron análisis, usando las dimensiones de vulnerabilidad a las ETS/VIH, y se realizaron modelos de regresión logística para estimar las odds ratios de la asociación entre las variables predictoras y la prueba treponémica positiva para la sífilis al comenzar el estudio. Se analizaron a 677 participantes. La media de edad fue 18,9 años (IQR: 18,1-19,5), 70,5% (477) se autodeclararon como negros, 70,5% (474) como homosexuales/gays y 48 (7,1%) como mujeres trans o travestis. La base de referencia de prevalencia de sífilis fue 21,3%. En el modelo final de regresión logística, se asociaron con una posibilidad más alta de sífilis: un episodio autoinformado de ETS en los últimos 12 meses (OR = 5,92; IC95%: 3,74-9,37), trabajadores sexuales (OR = 3,39; IC95%: 1,32-8,78) y < 11 años de estudio (OR = 1,76; IC95%: 1,13-2,74). La prevalencia de sífilis entre adolescentes HSH/TrTGW con edades de 15 a 19 años fue alarmante, mucho más que lo descrito para la población general en este rango de edad y asociado con factores de vulnerabilidad. Esto refuerza la urgente necesidad de fortalecer los programas de salud pública, discutiendo cuestiones de raza, género, sexualidad y prevención.

Syphilis has reemerged as a serious public health problem in Brazil and worldwide, disproportionately affecting men who have sex with men (MSM) and travestis and transgender women (TrTGW). Studies on sexually transmitted infections (STI) in adolescents from these key populations are relatively scarce. This is a Brazilian multi-center, cross study with prevalence analysis, using as baseline the PrEP1519 cohort of sexually active MSM and TrTGW adolescents, recruited from April 2019 to December 2020. Analyses were made using the dimensions of vulnerability to STI/HIV and logistic regression models were conducted to estimate the odds ratios of the association between the predictor variables and positive treponemal test for syphilis at the moment of entry in the study. In total, 677 participants were analyzed; participants’ median age was 18.9 years (IQR: 18.1-19.5); 70.5% (477) self-declared as black; 70.5% (474), as homosexuals/gays; and 48 (7.1%), as trans women or travestis. The baseline prevalence of syphilis was 21.3%. In the final logistic regression model, higher chance of syphilis was associated with: self-reported episode of STI in the last 12 months (OR = 5.92; 95%CI: 3.74-9.37), sex worker (OR = 3.39; 95%CI: 1.32-8.78), and < 11 years of schooling (OR = 1.76; 95%CI: 1.13-2.74). The prevalence of syphilis among MSM/TrTGW adolescents aged from 15 to 19 years was alarming, much higher than the described for the general population within this age range and associated with vulnerability factors. This reinforces the urgent need to strengthen public health programs to debate about race, gender, sexuality, and prevention.

https://doi.org/10.1590/0102-311xen118721

Prevalence of syphilis and sexual behavior and practices among adolescents MSM and TrTGW in a Brazilian multi-center cohort for daily use of PrEP
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Westin, Mateus Rodrigues

; Martinez, Yuppiel Franmil

; Silva, Ana Paula

; Greco, Marília

; Marques, Lucas Miranda

; Campos, Guilherme Barreto

; Alves, Matheus de Paula

; Mancuzzo, Alessandra

; Tupinambás, Unaí

; Greco, Dirceu Bartolomeu

Cadernos de Saúde Pública

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2023, Volume 39 Suppl. 1 elocation e00118721

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

https://doi.org/10.1590/0102-311xen118721

Estatística Cardiovascular – Brasil 2021
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Oliveira, Gláucia Maria Moraes de

; Brant, Luisa Campos Caldeira

; Polanczyk, Carisi Anne

; Malta, Deborah Carvalho

; Biolo, Andreia

; Nascimento, Bruno Ramos

; Souza, Maria de Fatima Marinho de

; Lorenzo, Andrea Rocha De

; Fagundes Júnior, Antonio Aurélio de Paiva

; Schaan, Beatriz D.

; Castilho, Fábio Morato de

; Cesena, Fernando Henpin Yue

; Soares, Gabriel Porto

; Xavier Junior, Gesner Francisco

; Barreto Filho, Jose Augusto Soares

; Passaglia, Luiz Guilherme

; Pinto Filho, Marcelo Martins

; Machline-Carrion, M. Julia

; Bittencourt, Marcio Sommer

; Pontes Neto, Octavio M.

; Villela, Paolo Blanco

; Teixeira, Renato Azeredo

; Sampaio, Roney Orismar

; Gaziano, Thomaz A.

; Perel, Pablo

; Roth, Gregory A.

; Ribeiro, Antonio Luiz Pinho

Arquivos Brasileiros de Cardiologia

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jan 2022, Volume 118 N. 1 Pages 115 - 373

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20211012

Ovarian hormones influence immune response to Staphylococcus aureus infection
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Souza, Clarissa Leal Silva e

; Oliveira, Hellen Braga Martins

; Santos Júnior, Manoel N.

; Silva, Mariângela de Oliveira

; Coqueiro, Igor Lopes

; Silva, Ícaro Bonyek Santos da

; Campos, Guilherme Barreto

; Silva, Robson Amaro Augusto da

; Soares, Telma de Jesus

; Oliveira, Márcio Vasconcelos de

; Timenetsky, Jorge

; Marques, Lucas Miranda

Brazilian Journal of Infectious Diseases

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dec 2020, Volume 24 N. 6 Pages 534 - 544

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: Staphylococcus aureus infections remain associated with considerable morbidity and mortality in both hospitals and the community. There is little information regarding the role of ovarian hormones in infections caused by S. aureus. The aim of this study was to evaluate the effects of ovariectomy in the immune response induced by S. aureus. Methods: Female mice BALB/c were ovariectomized (OVX) to significantly reduce the level of ovarian hormones. We also used sham-operated animals. The mice were inoculated intraperitoneally with S. aureus. Blood samples were collected for leukocyte count and bacterial quantification. The uterus and spleen were removed and weighed to calculate the uterine and splenic indexes. Lungs were removed and fractionated for immunohistochemical analysis for macrophage detection (anti-CD68) and relative gene expression of IL-6, IL-1β and TNF-α by RT-PCR. Results: Ovariectomy enlarged spleen size and generally increased circulating lymphocytes. OVX females experienced a continuation of the initial reduction of lymphocytes and a monocyte and neutrophil late response compared to shams (p ≥ 0.05). Moreover, OVX females showed neutropenia after 168 h of infection (p ≥ 0.05). Macrophage response in the lungs were less pronounced in OVX females in the initial hours of infection (p ≥ 0.01). OVX females showed a higher relative gene expression of IL-1β, IL-6 and TNF-α in the lung at the beginning of the infection compared to sham females (p ≥ 0.01). Among the uninfected females, the OVX control females showed a higher expression of IL-6 in the lung compared to the sham control females (p ≥ 0.05). In this model, the lack of ovarian hormones caused a minor increase in circulating leukocytes during the initial stage of infection by S. aureus and increased pulmonary gene expression of IL-1β, IL-6, and TNF-α. Ovariectomy alone enlarged the spleen and increased circulating lymphocytes. Ovarian hormones acted as immunoprotectors against S. aureus infection.

https://doi.org/10.1016/j.bjid.2020.10.004 338 downloads

RELATIONSHIP BETWEEN THE NATIONAL EVALUATION SYSTEM AND THE PERFORMANCE OF BRAZILIAN ROWERS
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Silva, Fábio Barreto Maia da

; Campos, Marcela Sacramento

; Soares, Guilherme Ribeiro

; Gomes, Antonio Carlos

; Torres, Fernando Carmelo

Revista Brasileira de Medicina do Esporte

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dec 2020, Volume 26 N. 6 Pages 547 - 550

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Introdução: O Sistema Nacional de Avaliação do Remador (SNAR), adotado pela Confederação Brasileira de Remo, tem como finalidade estabelecer um ranking nacional, sendo pré-requisito à participação em Campeonatos e Seletivas Nacionais, em suas diferentes categorias, bem como para ingresso na Seleção Brasileira de Remo. Objetivo: Este estudo visou analisar a relação e a predição dos resultados do SNAR com os tempos dos competidores num Campeonato Brasileiro de Remo (CBR). Métodos: A investigação envolveu 11 remadoras (18,00 ± 0,89 anos) e 16 remadores (18,18 ± 0,91 anos), participantes de um CBR, na categoria júnior. A pesquisa foi qualitativa, com análise de conteúdo dos dados disponíveis no site da Confederação. Resultados: Na categoria júnior feminino, das dez variáveis estudadas, três apresentaram correlação r ≥ 0,50; na categoria júnior masculino, nove apresentaram correlação r ≥ 0,50. A equação de regressão linear múltipla, com todas as variáveis estudadas, apresentou R2 = 0,86 e SEE = 5,30, na categoria feminino; R2 = 0,90 e SEE = 3,56, na categoria masculino. Conclusão: Os resultados obtidos sugerem que a análise dos testes indicados pelo SNAR pode ser uma fonte importante de informações, oferecendo subsídios relevantes a dirigentes, atletas e comissão técnica, tanto de diagnóstico de desempenho como, especialmente, de prognóstico em competição, além de permitir eventuais adaptações no planejamento de treino, quando necessárias e possíveis. Nível de vidência I; Estudos diagnósticos–Investigação de um exame para diagnóstico.

ABSTRACT Introduction: The National Rower Evaluation System [Sistema Nacional de Avaliação do Remador] (SNAR), adopted by the Brazilian Rowing Confederation, aims to establish a national ranking, and is a prerequisite to participation in National Championships and Selective Games, in its different categories, as well as for joining the Brazilian Rowing Team. Objective: This study aimed to analyze the relationship and the prediction of the SNAR results with competitors’ times in a Brazilian Rowing Championship (CBR). Methods: The investigation involved 11 female rowers (18.00 ± 0.89 years) and 16 male rowers (18.18 ± 0.91 years), participants in a CBR, in the junior category. The research was qualitative, with content analysis of the data available on the Confederation's website. Results: In the junior female category, of the ten variables studied, three showed a correlation r≥0.50; in the junior male category, nine showed a correlation r≥0.50. The multiple linear regression equation, with all the variables studied, showed R2 = 0.86 and SEE = 5.30, in the female category; and R2 = 0.90 and SEE = 3.56, in the male category. Conclusion: Based on our results, the tests indicated by SNAR can be an important source of information, offering significant support for managers, athletes and coaching staff, for use in performance diagnosis and in particular, competition prognosis. It can also be used to adapt training schedule where necessary. Level of evidence I; Diagnostic studies-Investigation of a diagnostic test.

RESUMEN Introducción: El Sistema Nacional de Evaluación del Remador (SNAR), adoptado por la Confederación Brasileña de Remo, tiene como finalidad establecer un ranking nacional, siendo un requisito previo para la participación en Campeonatos y Selectivas Nacionales, en sus diferentes categorías, bien como para ingreso en la Selección Brasileña de Remo. Objetivo: Este estudio tuvo como objetivo analizar la relación y la predicción de los resultados del SNAR con los tiempos de los competidores en un Campeonato Brasileño de Remo (CBR). Métodos: La investigación involucró a 11 remadoras (18,00 ± 0,89 años) y a 16 remadores (18,18 ± 0,91 años), participantes de un CBR, en la categoría junior. La investigación fue cualitativa, con análisis de contenido de los datos disponibles en el sitio web de la Confederación. Resultados: En la categoría junior femenina, de las diez variables estudiadas, tres presentaron correlación r ≥ 0,50; en la categoría junior masculina, nueve presentaron correlación r ≥ 0,50. La ecuación de regresión lineal múltiple, con todas las variables estudiadas, presentó R2 = 0,86 y SEE = 5,30, en la categoría femenina; R2 = 0,90 y SEE = 3,56, en la categoría masculina. Conclusión: Los resultados obtenidos sugieren que el análisis de los tests indicados por el SNAR puede ser una fuente importante de informaciones, ofreciendo subsidios relevantes a los dirigentes, atletas y a la comisión técnica, tanto de diagnóstico de desempeño como, especialmente, de pronóstico en competición, además de permitir eventuales adaptaciones en la planificación de entrenamiento, cuando sea necesario y posible. Nivel de evidencia I; Estudios diagnósticos-investigación de un examen para diagnóstico.

https://doi.org/10.1590/1517-869220202606214979 681 downloads

Desafios e propostas para ampliação da testagem e diagnóstico para COVID-19 no Brasil
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Magno, Laio

; Rossi, Thais Aranha

; Mendonça-Lima, Fernanda Washington de

; Santos, Carina Carvalho dos

; Campos, Guilherme Barreto

; Marques, Lucas Miranda

; Pereira, Marcos

; Prado, Nilia Maria de Brito Lima

; Dourado, Inês

Ciência & Saúde Coletiva

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sep 2020, Volume 25 N. 9 Pages 3355 - 3364

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O contexto brasileiro de desigualdades sociais e barreiras no acesso aos serviços de saúde pode agravar a situação da pandemia de COVID-19, que já afeta todos os estados da federação, com a curva crescente de aumento de casos confirmados e mortes. O governo dos países e os agentes do campo científico têm buscado evidências para as melhores práticas de prevenção e controle da transmissão, e cuidado da infecção e doença, incluindo medidas de diagnóstico, tratamento e de atenção à saúde. A estratégia de testagem em larga escala, visando o diagnóstico precoce, quarentena dos casos leves identificados, bem como dos contactantes, e cuidado adequado dos casos graves, tem sido revisada e indicada como uma das medidas eficientes para o controle da pandemia em vários países do mundo. O artigo tem como objetivo discutir os desafios da testagem e do diagnóstico de COVID-19 no Brasil.

Abstract The Brazilian context of social inequalities and barriers in accessing health services may deteriorate the situation of the COVID-19 pandemic, which already affects all Brazilian federative states, with the growing curve of increasing confirmed cases and deaths. National governments and scientific field agents have been looking for evidence for the best practices of prevention and control of transmission, and care of infection and disease, including diagnosis, treatment, and health care measures. The large-scale testing strategy, aimed at early diagnosis, quarantine of the mild cases identified, as well as those of the contacts, and adequate care of severe cases, has been revised and indicated as one of the efficient pandemic control measures in several countries in the world. This paper aims to discuss the challenges of COVID-19 testing and diagnosis in Brazil.

https://doi.org/10.1590/1413-81232020259.17812020 7330 downloads

Effectiveness of the Aloe Vera extract in the treatment of fistula-in-ano
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Cavasana, Antonio Luis

; Santos, Carlos Henrique Marques dos

; Dourado, Doroty Mesquita

; Guimarães, Felipe dos Santos

; Barros, Fernanda Helene Reis

; de Campos, Gattass Carneiro Orro

; Leme, Guilherme Apolinário Laureano

; Silva, Lucas Dutra Madrid da

; Wahl, Lucas Marques

; Gutterres, Nathália Barreto de Almeida

; Matias, Rosemary

; Câmara, Sara Jéssica Falcão

; Santos, Sandro Endrick de Oliveira

Journal of Coloproctology (Rio de Janeiro)

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mar 2020, Volume 40 N. 1 Pages 67 - 72

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Racional Considerando que o tratamento das fístulas anais tem risco de incontinência fecal e o crescente uso do Aloe Vera na medicina, há interesse em se pesquisar este fármaco. Objetivo Avaliar a eficácia do extrato de Aloe vera no tratamento das fístulas anais em ratos. Método Utilizou-se 30 ratos Wistar, os quais foram submetidos à criação de fístula anal e após 30 dias distribuídos em três grupos: Controle (GCo), Carbopol (GCa) e Aloe Vera (GAV). No GCo nenhum tratamento foi realizado, enquanto nos outros realizou-se infusão diária de 0,3 mL de Carbopol GCa e Carbopol mais extrato de Aloe Vera no GAV por 30 dias. Foram retirados os espécimes para estudo histológico, avaliou-se o fechamento do trajeto fistuloso, a área do trajeto remanescente, o infiltrado inflamatório e o grau de congestão vascular. Resultados Não houve fechamento completo do trajeto fistuloso em nenhum dos animais. A média da área do trajeto remanescente foi 847,2 µm no GCo; 565,6 µm no GCa e 377,8 µm no GAV (p < 0,05). A média do escore de infiltrado inflamatório foi 2,4 no GCo; 2,4 no GCa e 2,3 no GAV (p > 0.05), enquanto na avaliação da congestão vascular observou-se média 1,6 no GCo; 1,4 no GCa e 1,1 no GAV (p < 0,05). Conclusão O extrato de Aloe Vera foi capaz de diminuir o lumen dos trajetos fistulosos e reduzir o grau de congestão vascular, porém, não permitiu o fechamento completo dos trajetos fistulosos nem diminuiu o processo inflamatório.

Abstract Rationale Considering that anal fistulae are still challenging regarding their treatment due to the risk of fecal incontinence in the most complex cases and the increasing use of phytotherapeutic drugs such as Aloe Vera in medicine, even with proven healing effectiveness, there is interest in researching this drug in the treatment of anal fistulae. Objective To evaluate the efficacy ofAloe Vera extract in the treatment of anal fistulae in rats. Method Thirty male Wistar rats weighing 250–300 g were submitted to anal fistula and after 30 days were divided into three groups: Control Group (GCo) (n = 5), Carbopol Group (GCa) (n = 5), and Aloe Vera Group (GAV) (n = 10). In the GCo no treatment was performed, while in the others a daily infusion of 0.3 mL of solution (Carbopol only in GCa and Carbopol plus Aloe Vera extract in the GAV) was performed through the external orifice of the fistula for 30 days. Afterwards, euthanasia was performed and specimens were removed for histological study. It was evaluated the closure of the fistulous tract, the area of the remaining tract, the inflammatory infiltrate and the degree of vascular congestion. The results were submitted to statistical treatment by Kruskall–Wallis test, considering p < 0.05. Results There was no complete closure of the fistulous tract in any of the animals. The mean area of the remaining tract was 847.2 µm in the GCo, 565.6 µm in the GCa and 377.8 µm in the GAV (p < 0.05). The mean of the inflammatory infiltrate score was 2.4 in the GCo, 2.4 in the GCa and 2.3 in the GAV (p > 0.05), while in the evaluation of vascular congestion, we observed a mean of 1.6 in the GCo, 1.4 in GCa and 1.1 in GAV (p < 0.05). Conclusion The extract of Aloe vera was able to reduce the lumen of the fistulous tract and reduce the degree of vascular congestion; however, it did not allow the complete closure of the fistulous tract nor diminished the inflammatory process.

https://doi.org/10.1016/j.jcol.2019.09.003 1169 downloads

Desafios e propostas para ampliação da testagem e diagnóstico para COVID-19 no Brasil
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Magno, Laio

; Rossi, Thais Aranha

; Mendonça-Lima, Fernanda Washington de

; Santos, Carina Carvalho dos

; Campos, Guilherme Barreto

; Marques, Lucas Miranda

; Pereira, Marcos

; Prado, Nilia Maria de Brito Lima

; Dourado, Inês

Ciência & Saúde Coletiva

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2020, Volume 25 N. 9 Pages 3355 - 3364

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1413-81232020259.17812020

Tratamento da fístula anal com extrato de Baccharis dracunculifolia. Estudo experimental em ratos.
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Santos, Carlos Henrique Marques dos

; Cavasana, Antonio Luís

; Moreira, Davyson de Lima

; Dourado, Doroty Mesquita

; Guimarães, Felipe dos Santos

; Barros, Fernanda Helene Reis

; Campos, Gattass Carneiro Orro de

; Leme, Guilherme Apolinário Laureano

; Silva, Lucas Dutra Madrid da

; Wahl, Lucas Marques

; Gutterres, Nathália Barreto de Almeida

; Matias, Rosemary

; Câmara, Sara Jéssica Falcão

; Santos, Sandro Endrick de Oliveira

Revista do Colégio Brasileiro de Cirurgiões

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2019, Volume 46 N. 5 elocation e20192297

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: avaliar a eficácia do extrato de Baccharis dracunculifolia no tratamento de fístulas anais em ratos. Métodos: vinte ratos Wistar machos foram submetidos à confecção de fístula anal e, após 30 dias, foram distribuídos em três grupos: Grupo Controle, com cinco animais; Grupo Carbopol, com cinco animais; e Grupo Baccharis dracunculifolia, com dez animais. No Grupo Controle, não se realizou nenhum tratamento. No Grupo Carbopol, realizou-se infusão diária de carbopol, e no Grupo Baccharis dracunculifolia, infusão de extrato de Baccharis dracunculifolia com carbopol, ambos por 30 dias. Foram retirados espécimes para análise histológica após a eutanásia. Resultados: não houve fechamento completo do trajeto fistuloso em nenhum dos animais. A média da área do trajeto resultante foi de 847,2µm2, 565,6µm2 e 372,7µm2, respectivamente, nos Grupos Controle, Carbopol e Baccharis dracunculifolia (p=0,001). A média do escore de processo inflamatório foi de 2,4, 2,4 e 2,1, respectivamente, nos Grupos Controle, Carbopol e Baccharis dracunculifolia (p=0,285), enquanto a média de congestão vascular foi de 1,6, 1,4 e 1,1, respectivamente, nos Grupos Controle, Carbopol e Baccharis dracunculifolia (p=0,031). Conclusão: o extrato de Baccharis dracunculifolia foi capaz de reduzir o lúmen dos trajetos fistulosos e a congestão vascular, sem reduzir, no entanto, o processo inflamatório local ou fechar totalmente os trajetos fistulosos.

ABSTRACT Objective: to evaluate the efficacy of Baccharis dracunculifolia extract in the treatment of anal fistulas in rats. Methods: twenty male Wistar rats were submitted to anal fistula and, after 30 days, were divided into three groups: Control Group, with five animals; Carbopol Group, with five animals; and Baccharis dracunculifolia Group, with ten animals. In the Control Group, no treatment was performed. In the Carbopol Group, a daily infusion of Carbopol was performed for 30 days. In the Baccharis dracunculifolia Group, a daily infusion of Carbopol plus Baccharis dracunculifolia extract was performed for 30 days. Specimens were taken for histological analysis after euthanasia. Results: there was no complete closure of the fistulous tract in any of the animals. The mean area of the remaining tract was of 847.2µm2, 565.6µm2 and 372.7µm2, in the Control Group, Carbopol Group, and Baccharis dracunculifolia Group, respectively, (p=0.001). The mean of the inflammatory process score was of 2.4, 2.4, and 2.1, in the Control Group, Carbopol Group, and Baccharis dracunculifolia Group, respectively, (p=0.285), while the mean values of vascular congestion were of 1.6, 1.4, and 1.1, in the Control Group, Carbopol Group, and Baccharis dracunculifolia Group, respectively, (p=0.031). Conclusion: Baccharis dracunculifolia extract was able to reduce the lumen of the fistulous tracts and the degree of vascular congestion, without, however, reducing the local inflammatory process or totally closing the fistulous tracts.

https://doi.org/10.1590/0100-6991e-20192297 6432 downloads

10.

Apoptosis in HEp-2 cells infected with Ureaplasma diversum
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Amorim, Aline Teixeira

; Marques, Lucas Miranda

; Santos, Angelita Maria Oliveira Gusmão

; Martins, Hellen Braga

; Barbosa, Maysa Santos

; Rezende, Izadora Souza

; Andrade, Ewerton Ferraz

; Campos, Guilherme Barreto

; Lobão, Tássia Neves

; Cortez, Beatriz Araujo

; Monezi, Telma Alvez

; Machado-Santelli, Glaucia Maria

; Timenetsky, Jorge

Biological Research

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2014, Volume 47 Pages 1 - 9

Abstract: En | Text: En | PDF: En

BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. RESULTS: The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. CONCLUSIONS: The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS). Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells.

https://doi.org/10.1186/0717-6287-47-38 1090 downloads

11.

Classification of journals in the QUALIS System of CAPES URGENT need of changing the criteria!
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Andriolo, Adagmar

; Souza, Aécio Flávio Meireles

; Farias, Alberto Queiroz

; Barbosa, Alfredo José Afonso

; França Netto, Antonio Spina

; Hernandez, Arnaldo José

; Camargos, Aroldo F.

; Barraviera, Benedito

; Kadunc, Bogdana Victoria

; Caramelli, Bruno

; Campos, Carlos Eduardo Aguilera

; Brites, Carlos

; Nascimento, Dejair Caitano do

; Braile, Domingo M.

; Goldenberg, Dov Charles

; Baracat, Edmund Chada

; Kimura, Edna T.

; Marchiori, Edson

; Vieira, Eduardo de Paula

; Almeida, Eros Antônio de

; Jotz, Geraldo Pereira

; Camanho, Gilberto

; Friedman, Gilberto

; Cerri, Giovanni Guido

; Duarte, Ivomar Gomes

; Costa, Izelda Maria Carvalho

; Mello Júnior, João Ferreira de

; Faintuch, Joel

; Martinez, José Antônio Baddini

; Antonio Livramento, José

; Manso, José Eduardo Ferreira

; Amaral, José Luiz Gomes do

; Battistella, Linamara Rizzo

; Machado, Luís dos Ramos

; Moreira, Luiz Felipe P.

; Gebrim, Luiz Henrique

; Madeira, Marcelo

; Riberto, Marcelo

; Bastos, Marcus

; Falcão, Mário Cícero

; Conceição, Mario J. da

; Silva, Mauricio Rocha e

; Ruiz, Milton Artur

; Shibata, Milton K.

; Santiago, Mittermayer Barreto

; Andreollo, Nelson Adami

; Malafaia, Osvaldo

; Martins, Regina Helena Garcia

; Procianoy, Renato Soibelmann

; Baroudi, Ricardo

; Fuller, Ricardo

; Viebig, Ricardo Guilherme

; Nitrini, Ricardo

; Moura, Rita Cristina Mainieri R. de

; Dedivitis, Rogério

; Damião, Ronaldo

; Lianza, Sergio

; Rode, Sigmar de Mello

; Yoshida, Winston Bonetti

; Handar, Zuher

Arquivos de Neuro-Psiquiatria

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apr 2010, Volume 68 N. 2 Pages 327 - 329

Abstract: En | Text: En | PDF: En

3236 downloads Cited 9 times in SciELO

12.

Classificação dos Periódicos no Sistema QUALIS da CAPES - a Mudança dos Critérios é URGENTE!
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Andriolo, Adagmar

; Souza, Aécio Flávio Meireles

; Farias, Alberto Queiroz

; Barbosa, Alfredo José Afonso

; França Netto, Antonio Spina

; Hernandez, Arnaldo José

; Camargos, Aroldo F.

; Barraviera, Benedito

; Kadunc, Bogdana Victoria

; Caramelli, Bruno

; Campos, Carlos Eduardo Aguilera

; Brites, Carlos

; Nascimento, Dejair Caitano do

; Braile, Domingo M.

; Goldenberg, Dov Charles

; Kimura, Edna T

; Marchiori, Edson

; Vieira, Eduardo de Paula

; Almeida, Eros Antônio de

; Jotz, Geraldo Pereira

; Camanho, Gilberto

; Friedman, Gilberto

; Cerri, Giovanni Guido

; Duarte, Ivomar Gomes

; Costa, Izelda Maria Carvalho

; Mello Júnior, João Ferreira de

; Faintuch, Joel

; Martinez, José Antônio Baddini

; Livramento, José Antonio

; Manso, José Eduardo Ferreira

; Battistella, Linamara Rizzo

; Machado, Luís dos Ramos

; Moreira, Luiz Felipe P.

; Gebrim, Luiz Henrique

; Madeira, Marcelo

; Riberto, Marcelo

; Bastos, Marcus

; Falcão, Mário Cícero

; Conceição, Mario J. da

; Rocha e Silva, Mauricio

; Ruiz, Milton Artur

; Shibata, Milton K.

; Santiago, Mittermayer Barreto

; Andreollo, Nelson Adami

; Malafaia, Osvaldo

; Martins, Regina Helena Garcia

; Procianoy, Renato Soibelmann

; Baroudi, Ricardo

; Fuller, Ricardo

; Viebig, Ricardo Guilherme

; Nitrini, Ricardo

; Moura, Rita Cristina Mainieri R. de

; Dedivitis, Rogério

; Damião, Ronaldo

; Lianza, Sergio

; Rode, Sigmar de Mello

; Yoshida, Winston Bonetti

; Handar, Zuher

Arquivos Brasileiros de Cardiologia

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mar 2010, Volume 94 N. 3 Pages 290 - 291

Abstract: Pt | Text: Pt | PDF: Pt

3995 downloads Cited 2 times in SciELO

13.

Classificação dos periódicos no Sistema Qualis da Capes: a mudança dos critérios é urgente!
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Andriolo, Adagmar

; Barbosa, Alfredo José Afonso

; França Netto, Antonio Spina

; Hernandez, Arnaldo José

; Camargos, Aroldo F.

; Barraviera, Benedito

; Kadunc, Bogdana Victoria

; Caramelli, Bruno

; Campos, Carlos Eduardo Aguilera

; Brites, Carlos Roberto

; Braile, Domingo M.

; Goldenberg, Dov Charles

; Kimura, Edna T

; Marchiori, Edson

; Vieira, Eduardo de Paula

; Sampaio, Francisco J. B.

; Jotz, Geraldo Pereira

; Camanho, Gilberto

; Friedman, Gilberto

; Cerri, Giovanni Guido

; Duarte, Ivomar Gomes

; Costa, Izelda Maria Carvalho

; Mello Júnior, João Ferreira de

; Faintuch, Joel

; Martinez, José Antônio Baddini

; Livramento, José Antonio

; Manso, José Eduardo Ferreira

; Battistella, Linamara Rizzo

; Machado, Luís dos Ramos

; Moreira, Luiz Felipe P.

; Gebrim, Luiz Henrique

; Madeira, Marcelo

; Riberto, Marcelo

; Bastos, Marcus

; Falcão, Mário Cícero

; Silva, Mauricio Rocha e

; Ruiz, Milton Artur

; Shibata, Milton K.

; Santiago, Mittermayer Barreto

; Martins, Regina Helena Garcia

; Procianoy, Renato Soibelmann

; Baroudi, Ricardo

; Fuller, Ricardo

; Viebig, Ricardo Guilherme

; Nitrini, Ricardo

; Dedivitis, Rogério

; Damião, Ronaldo

; Lianza, Sergio

; Rode, Sigmar de Mello

; Yoshida, Winston Bonetti

; Handar, Zuher

Revista Brasileira de Hematologia e Hemoterapia

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feb 2010, Volume 32 N. 1 Pages 1 - 3

Abstract: Pt | Text: Pt | PDF: Pt

3073 downloads Cited 2 times in SciELO

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