Abstract Herbicide mineralization in the soil is affected by several factors, including the herbicide application history of the soil, soil moisture, and whether the herbicide is co-applied with another herbicide. The objective of this work was to evaluate S-metolachlor mineralization in the soil as affected by soil moisture content, history of herbicide application, and association with glyphosate. 14C-S-metolachlor mineralization increased with increasing soil moisture content. The average cumulative mineralization of S-metolachlor at 63 days of incubation was 0.03, 0.80, and 1.80% in air-dried, field capacity, and saturated soil, respectively. The mineralization rate of S-metolachlor was greater under saturated conditions and affected by the association with glyphosate. Greater mineralization of S-metolachlor occurred in cornfield soil with a history of S-metolachlor application. The average cumulative mineralization was 0.24% in cornfield and 0.89% in non-cultivated area. We can conclude that the higher the soil moisture content the higher S-metolachlor mineralization and that the mixture of this herbicide with glyphosate increased its degradation compared to the application of the herbicide alone. While there is evidence suggesting enhanced degradation of S-metolachlor in soil previously subjected to corn cultivation and S-metolachlor application, drawing a definitive conclusion is challenging due to the influence of sorption observed in our dataset.

ABSTRACT In order to establish minimum criteria related to social innovation in agricultural community organizations, qualitative research tools were used, including specialized searches and the use of programs for the analysis of bibliographic data (for example VOSviewer™), with the purpose of defining the elements related to management, governance, capacities, business model and sustainability for eight (8) community organizations of agricultural producers in the province of Sugamuxi, in the department of Boyacá, Colombia. It was possible to establish that some organizations of agricultural producers that carry out activities in the department of Boyacá are not integrally appropriating the elements of social innovation (at operational and managerial levels) and, consequently, there is a whole route of improvement to be developed with them to optimize the organizational efficiency indexes; in addition, the complexity of the subject is evident, given that the sustainability of social innovations will depend largely on the management of the organizations, where shared institutional agendas contextualized and adjusted to the territories are established. It is necessary to optimize the channels of participation in a joint manner between the State, the organizations, and the producers themselves to be able to dynamize processes, goals and the real scope of social innovation in light of the accelerated changes faced by the agribusiness producer in Colombia, related to market dynamics, access to resources and others.

RESUMEN Con el objetivo de establecer criterios mínimos relativos a la innovación social en organizaciones comunitarias agropecuarias, se hizo uso de herramientas propias de la investigación cualitativa que incluyeron búsquedas especializadas y uso de programas para análisis de datos bibliográficos (por ejemplo VOSviewer™) para determinar los elementos relativos a la gestión, gobernanza, capacidades, modelo de negocio sostenibilidad para ocho (8) organizaciones comunitarias de productores agropecuarios de la provincia del Sugamuxi, en el departamento de Boyacá, Colombia. Se pudo establecer que algunas organizaciones de productores agropecuarios que ejercen actividades en el departamento de Boyacá no están apropiando de forma integral los elementos de innovación social (en niveles operativos y gerenciales) y, consecuentemente, existe toda una ruta de mejoramiento por desarrollar con estas para optimizar los índices de eficiencia organizacional; además, se evidencia la complejidad de la temática, dado que la sostenibilidad de las innovaciones sociales dependerá en gran medida de la gestión de las organizaciones, donde se establezcan agendas institucionales compartidas contextualizadas y ajustadas a los territorios. Es necesario optimizar los canales de participación de manera mancomunada entre el Estado, las organizaciones y los mismos productores para poder dinamizar procesos, metas y alcances reales de la innovación social a la luz de los acelerados cambios a los que se enfrenta el productor agropecuario agremiado en Colombia, relativos estos a la dinámica del mercado, el acceso a recursos y otros.

Three new triterpenes, 3,4-seco-3,11β-epoxyfriedel-4(23)-en-3β-ol (1), friedelan-3α,11β diol (2), 7β,26-epoxyfriedelan-3a,7a-diol (3), a mixture of two new triterpenes 3α-hydroxyfriedelan-29-yl palmitate (4) and 3α-hydroxyfriedelan-29-yl stearate (5) and eleven known compounds were obtained from the hexane extract of Maytenus quadrangulata leaves. The structures and the relative stereochemistry of the new triterpenes were established through 1D/2D nuclear magnetic resonance (NMR), high resolution mass spectrometry (HRMS), and Fourier transform infrared (FTIR) spectral data. The hexane extract and isolated compounds were submitted to the cytotoxicity assays against leukemia (THP-1 and K562), ovarian (TOV-21G) and breast cancer (MDA-MB-231) cell lines. Compounds 1, 2 and 11β-hydroxyfriedelan-3-one (15) displayed high cytotoxicity and selectivity against leukemic cells when compared to positive control cytarabine (for THP-1) and imatinib (for K562). Furthermore, compound 2 showed similar cytotoxicity and an enhanced selectivity towards ovarian and breast cells in comparison to positive control etoposide.

RESUMO O Rio Grande do Sul apresenta baixa oferta de milho em relação à demanda, condição agravada com limitação hídrica. Assim, objetivou-se estimar o ganho de produtividade do milho utilizando irrigação. O modelo da zona agroecológica da FAO, calibrado e validado, foi utilizado para simulação, considerando um experimento de blocos casualizados, com 40 safras de repetições, e 20 locais, manejo irrigado e sequeiro, três datas de semeadura (15/Ago, 15/Set e 15/Out) para solos de textura arenosa, média e argilosa. Um híbrido genérico obtido na calibração com base em vários híbridos com ciclo médio de 150 dias foi considerado na simulação. Na validação, o modelo apresentou um erro absoluto relativo de 16%, superestimando a produtividade em apenas 2%. A produtividade média irrigada e de sequeiro foi, respectivamente, de 16.094 e 5.386 kg ha-1. As datas de semeadura de 15 Set e 15 Out apresentaram valores estatisticamente superior para a condição irrigada. O uso da irrigação resultou em maior ganho de produtividade para 15 Set e 15 Out, com maior demanda de irrigação. O maior ganho de produtividade com uso de irrigação foi de 56%, em São Gabriel, com aumento de 14% na demanda de irrigação quando comparado 15 Out e 15 Ago. Os tipos de solos obtiveram diferenças significativas para a condição de sequeiro, enquanto que a irrigação minimizou as diferenças de produtividade, não apresentando diferença estatística. A irrigação demonstrou potencial para aumentar a oferta de milho, em que manejos locais podem ser considerados no plano agrícola.

ABSTRACT The state of Rio Grande do Sul, Brazil, has a low maize production when compared to the total demand, particularly under water deficit conditions. This study aimed to estimate the yield gain of maize using irrigation. The FAO Agroecological zone model was used to simulate the yield after previous calibration and evaluation, following an experimental design of randomized blocks, with 40 growing seasons as replicates and 20 sites. Two water management (rainfall and irrigation), three sowing dates (Aug 15, Sept 15, and Oct 15), and three soil textures (sandy, sand-clayey, and clayey) were evaluated. The generic hybrid obtained from calibration based on multiple hybrids with a medium cycle of 150 d was utilized for the simulation. The model evaluation showed an absolute bias of 16% and an overestimated yield of 2%. The mean irrigated and rainfed yields were, respectively, 16,094 and 5,386 kg ha-1. The irrigated yield had statistically superior values for the sowing dates Sep 15 and Oct 15, although it required a greater amount of irrigation. The yield gain reached a maximum value of 56% in the site of São Gabriel, with irrigation amount increasing 14% on the sowing date Oct 15 compared to that of Aug 15. The soil types showed statistical differences for rainfed conditions, and irrigation minimized the differences, while no statistically significant differences were found for the yield. Irrigation showed potential to increase the maize supply, and the response across sites can be considered in the agricultural management plan.

Abstract Ceramic additive manufacturing allows the fabrication of small series of complex parts without the high costs of molds usually associated with traditional ceramic processing. Although research into ceramic 3D printing by all technologies started back in the 90s, its industrial application is still quite restricted when compared to polymers and metals, which is related to the limited availability and costs of equipment and materials for such applications. This review examined the advantages and limitations of each process (binder jetting, direct ink writing, directed energy deposition, fused deposition, material jetting, selective laser sintering, selective laser melting, and vat photopolymerization), discussing their particularities. It also summarized the commercially available 3D printers and raw materials for ceramic processing, pointing out to trends and challenges of each technology.

There is a trend of development of analytical methodologies and technologies that allow in situ analysis, producing accurate information in real time, at low cost, using homemade experiments and devices to increase interest in scientific knowledge with a constructive approach in an interdisciplinary perspective in Chemistry Education. In this context, a photometric-chemometric method of analysis was developed to measure the pH of solutions using easily accessible and low-cost material based on the use of natural pigments found in red cabbage (Brassica oleracea L.). Calibrations and determinations were performed by RGB measurements of pigment coloration in solution at different pH values using the free app Photometrix, converted to HSV, YCbCr and YUV color spaces and processed by Partial Least Squares regression (PLS). The best PLS model found was HSV with mean central scale obtained pH measurements with RMSEP 0.98, R2 0.97 and bias close to zero. In addition, experimental data were statistically validated. Analyzes of predicted pH from three independents experiments revealed high recoveries (95-103%) and low relative standard deviations. Thus, the PhotoMetrix app was viable for colorimetric pH determinations using the low-cost photometric pH meter sensor and a smartphone, improving accessibility and applicability in Chemistry Education.

Abstract Acne is a chronic inflammatory disease of the pilosebaceous unit with multifactorial etiology. Abnormal proliferation of keratinocytes, altered sebum production, inflammation of the sebaceous follicle, and colonization by Cutibacterium acnes have been traditionally implicated. However, the diet has also been highlighted in the pathogenesis because of its direct relation with some biochemical markers and the transcription of specific genes associated with sebaceous gland activity, inflammation, and bacterial proliferation, which together promote the development of the disease, affect the severity of the condition, and modify its response to treatment.

Resumen El acné es una enfermedad inflamatoria crónica de la unidad pilosebácea de etiología multifactorial, en la que clásicamente se han implicado la proliferación anormal de queratinocitos, la producción alterada de sebo, la inflamación del folículo sebáceo y la colonización por Cutibacterium acnes. Sin embargo, también destaca la dieta en la patogenia al relacionarse directamente con la alteración de algunos marcadores bioquímicos y transcripción de ciertos genes que se asocian con la actividad de la glándula sebácea, la inflamación y la proliferación bacteriana, que en conjunto promueven el desarrollo de la enfermedad, afectan la gravedad del cuadro y modifican su respuesta al tratamiento.

Abstract: The coyote (Canis latrans) is a widespread predator with a high degree of adaptation to different ecosystems. The objective of this study was to estimate the relative abundance index, habitat selection, and diet of C. latrans through scent stations, in two types of vegetation located in northwestern Chihuahua. From April 2018 to March 2019, ten fixed scent stations (SS) were placed in pine-oak forest and other ten in semi-open grassland, with a linear separation of 500 meters between each station to achieve a five km transect in each vegetation during 12 samplings (two sampling night per month) for totaling 420 SS after discarding inactive SS. The relative abundance index of coyote showed that both types of vegetation, pine-oak forest (0.30) and semi-open grassland (0.23) were used in a similar way. The habitat selection test (Chi2) showed that coyote abundance and type of vegetation were independent (χ2 = 2.96, P > 0.05), not showing statistically significant differences in annual relative abundance index of coyotes between the two vegetation types. The food items detected in thirty-four scats collected belonged to mammals (55.9 %), fruits (35.3 %) and arthropods (8.8 %). Rodents and lagomorphs were the main source of food. Throughout the sampling period, the pine-oak forest showed the greatest relative abundance index of C. latrans. This may be because the forest provides them with shelter from climatic situations and a greater variety of seeds, fruits and insects. In this study the two seasons with the highest relative abundance were spring and autumn in both ecosystems, coinciding with an increase in rainfall, resulting in an abundance of potential prey (rodents and lagomorphs), and other food items like fruits. Regarding annual diet the most consumed prey were mammals. We found differences in diet between seasons, that can be explained by the variation in food availability among seasons.

Resumen: El coyote (Canis latrans) es un depredador ampliamente distribuido y con un alto grado de adaptación a diferentes ecosistemas. El objetivo de este estudio fue estimar el índice de abundancia relativa, selección de hábitat, y dieta de C. latrans a través de estaciones olfativas, en dos tipos de vegetación localizados en el noroeste de Chihuahua. A partir del mes de abril de 2018 hasta marzo de 2019, 10 estaciones olfativas (EO) fueron colocadas en bosque de pino-encino y otras 10 se colocaron en pastizal mediano abierto, con una separación lineal entre ellas de 500 metros entre cada estación para completar un transecto de cinco km en cada tipo de vegetación durante 12 muestreos (dos noches de muestreo por mes), totalizando 420 EO después de eliminar las EO inactivas. El índice de abundancia relativa para el coyote muestra que ambos tipos de vegetación, bosque de pino-encino (0.30) y el pastizal mediano abierto (0.23) fueron usados de forma similar. La selección de hábitat muestra (Chi2) un valor más bajo χ2 entre bosque y pastizal, sugiriendo que la abundancia y tipo de vegetación son independientes (2.96 P > 0.05), sin mostrar diferencias estadísticamente significativas en la abundancia relativa anual de coyotes entre los dos tipos de vegetación. Los alimentos detectados en 34 excretas recolectadas pertenecen a mamíferos (55.9 %), fruta (35.3 %) y artrópodos (8.8 %). Los roedores y lagomorfos fueron la principal fuente de alimento. A través del periodo de muestreo, el bosque de pino-encino mostro el índice de abundancia relativa más alto para C. latrans. Probablemente debido a que el bosque les provee de refugio ante situaciones climáticas y una gran diversidad de semillas, fruta e insectos. En este estudio las dos estaciones con la abundancia relativa más alta fueron primavera y otoño en ambos ecosistemas, coincidiendo con un incremento en la precipitación, resultando en una abundancia de presas potenciales (roedores y lagomorfos), y otros alimentos como fruta. Las presas más consumidas durante el año fueron mamíferos. Encontramos diferencias en dieta entre estaciones, que se puede explicar por la variación en disponibilidad de alimento entre estaciones.

Abstract Background: Osteopetrosis is a rare hereditary bone dysplasia characterized by insufficient osteoclast activity that results in increased bone mineral density. Hematopoietic stem cell transplantation (HSCT) can reverse skeletal abnormalities and restore hematopoiesis. Case report: We present the case of a 3-year and 2-month-old male patient with the diagnosis of osteopetrosis. The patient underwent allogeneic HSCT (Allo-HSCT) using 100% compatible bone marrow from a related donor and received a myeloablative conditioning regimen and a CD34 cell dose (4.7 × 107/kg). In the early post-transplant, frequent complications such as pneumonitis, hypercalcemia, and hyperphosphatemia ocurred. With a suitable granulocytic graft and chimerism of 100%, it was considered a successful transplant. However, the patient showed a delayed platelet graft treated with a platelet-stimulating factor for 6 months. The patient is currently disease-free, outpatient follow-up, with no data on graft-versus-host disease, and no progressive neurological damage. Conclusions: Osteopetrosis is a childhood disease that requires clinical suspicion and early diagnosis. HSCT is necessary at an early age to prevent disease progression and sensorineural, hematological, and endocrinological functions damage that can lead to death.

Resumen Introducción: La osteopetrosis es una displasia ósea hereditaria poco común, caracterizada por una actividad osteoclástica deficiente que aumenta la densidad mineral ósea. Se considera que el trasplante de células progenitoras hematopoyéticas (TCPH) puede revertir las anormalidades esqueléticas y restaurar la hematopoyesis. Caso clínico: Se presenta el caso de un paciente de sexo masculino, de 3 años y 2 meses de edad, con diagnóstico tardío de osteopetrosis. Se realizó un TCPH alogénico de donador relacionado 100% compatible con médula ósea. Se utilizaron un régimen de acondicionamiento mieloablativo y una dosis celular de CD34 de 4.7 × 107/kg de peso. En el postrasplante temprano, el paciente desarrolló complicaciones como neumonitis, hipercalcemia e hiperfosfatemia. Con un injerto granulocítico adecuado y quimerismo del 100% se consideró un trasplante exitoso. Sin embargo, el paciente presentó retraso en el injerto plaquetario, por lo que se administró factor estimulante de plaquetas por 6 meses. Actualmente el paciente se encuentra libre de enfermedad, en seguimiento ambulatorio, sin datos de enfermedad del injerto contra el hospedero y con pruebas de neurodesarrollo sin deterioro neurológico progresivo. Conclusiones: La osteopetrosis es una enfermedad infantil que requiere una sospecha clínica y un diagnóstico temprano, ya que es necesario un TCPH a corta edad como tratamiento para evitar la progresión de la enfermedad y el deterioro de las funciones neurosensoriales, hematológicas y endocrinológicas que puede derivar en la defunción del paciente.

RESUMO Objetivou-se neste estudo quantificar o efeito de variações da temperatura da água na vazão de fitas gotejadoras colapsáveis, de parede fina, com emissores integrados não regulados. Os ensaios foram conduzidos em laboratório utilizando estrutura de ensaios automatizada. Foram realizados ensaios para determinação das curvas vazão-pressão sob temperaturas de ensaio na faixa de 20 a 50 ºC, para nove modelos de emissores com três espessuras de parede (6, 8 e 9 MIL). Curvas vazão-pressão apresentaram distintos parâmetros K e x para cada temperatura. Para os emissores planos de fluxo turbulento (x < 0,5), a vazão diminuiu com o incremento da temperatura. Para os emissores contínuos de fluxo turbulento, respostas diferentes foram obtidas, sendo que no emissor D (x > 0,5) a vazão aumentou com o incremento da temperatura, enquanto no emissor C (x < 0,5), a vazão diminuiu e as maiores variações de vazão ocorreram a partir de 60 kPa. Para o emissor moldado, a vazão aumentou em função da temperatura, porém, a maior variação ocorreu nas menores pressões. Para nenhum dos emissores houve diferença significativa na variação de vazão entre as espessuras de parede.

ABSTRACT The objective of this study was to quantify the effect of water temperature variations on the discharge of collapsible thin-walled drip tapes with integrated non-pressure-compensating emitters. The tests were conducted in the laboratory using an automated test bench. Tests were performed to determine the discharge-pressure curves by varying the water temperature from 20 to 50 °C. Nine emitter models of three wall thicknesses (6, 8, and 9 MIL) were evaluated. The coefficients K and x of the discharge-pressure curves varied according to the water temperature. In flat emitters of turbulent flow (x < 0.5), the discharge decreased as the temperature increased. In the welded emitters of turbulent flow, several responses were observed. Regarding emitter D (x > 0.5), the discharge increased as the temperature was increased, while for emitter C (x < 0.5), the discharge decreased; the highest discharge variations occurred at pressures higher than 60 kPa. For embossed emitters, the discharge increased as a function of temperature, however, the greatest variation occurred at the lowest pressures. None of the emitters showed significant difference in the discharge variation due to wall thicknesses.

Abstract Candida spp. are naturally opportunistic and can promote infections. These yeasts can form biofilm, after penetration and adhesion to the biotic or abiotic surfaces. Preexisting diseases, treatments with drugs and radiation therapy, medical procedures, and parafunctional habits favor the installation of a fungal infection. Increased resistance to the available antifungals has become a concern. Therefore, alternative methods to control them have been evaluated, including the use of plant substances. In this study, the antibiofilm effect of R. officinalis L. extract was analyzed on C. albicans, C. dubliniensis, C. glabrata, C. krusei, and C. tropicalis. A phytochemical analysis of the extract was performed. Biofilms were formed for 48 h and exposed to the different concentrations of the extract (50, 100, and 200 mg/mL) for 5 min or 24 h. The effect of the plant extract was compared to the antifungal nystatin. Rosmarinus officinalis L. extract was constituted of phenols and flavonoids, highlighting the presence of chlorogenic acid derivatives in its composition. Biofilm reductions were observed after exposure to the plant extract for both periods. The plant extract provided a reduction similar to the antifungal. Thus, R. officinalis L. extract showed antibiofilm effect on Candida spp. comparable to the nystatin.

ABSTRACT The objective of this study was to evaluate the influence of minerals in the clay fraction on physical attributes of Yellow Ultisol cultivated with sugarcane and prepared with two different treatments: chiseling across the entire area and chiseling only in planting rows. The samples were collected from four layers of soil at eight months after planting. We assessed the texture of the soil, levels of iron extracted by dithionite-citrate-bicarbonate (DCB) and ammonium acid oxalate (AAO), ratio of kaolinite/(kaolinite + gibbsite), soil bulk density, and soil penetration resistance. The first area showed a higher ratio of kaolinite/(kaolinite + gibbsite) and the second area had greater levels of iron extracted by DCB. Levels of iron extracted by DCB were inversely correlated with soil bulk density and penetration resistance, while the kaolinite/(kaolinite + gibbsite) ratio was directly correlated with soil bulk density and penetration resistance. The area with a greater kaolinite/(kaolinite + gibbsite) ratio had higher values of soil bulk density and soil penetration resistance and the area with higher levels of iron showed lower values of soil bulk density and soil penetration resistance. The mineralogy influenced the soil's physical attributes, while soil preparation treatments did not.

Several studies have been carried out to develop new materials for biomedical applications. Material surfaces that present biomimetic morphology like nanotubes or nanofibers that provides nanoscale architectures have been shown to alter cell/biomaterial interactions. The coated surface biomaterial with biocompatible polymers and nanotubes of TiO2 is an alternative to improve osseointegration. The anodization process was performed to obtain nanotubes of TiO2 covering the Ti-30Ta alloy surface and the electrospinning process has been used for producing polymer fibers. Characterization techniques such as scanning electron microscopy (SEM - FEG), X-ray diffraction analysis (X-rays), thermogravimetric analysis (TGA), Differential Scanning Calorimetry (DSC) and contact angle were used for samples analyses. Adult human adipose-derived stem cells (ADSCs) were used to investigate the cellular response and S. aureus antimicrobial activity on these coated surfaces. The results indicated that both surface modification treatment showed a favorable micro-environment for cells growth and proliferation such as adhesion, viability and morphology which is a desire property for an implant. In addition, the antimicrobial activity study presented both materials with similar growth of S. aureus. So, it can conclude nanotubes and nanofibers can be used at biomedical field and both present similar cell evaluation and antimicrobial activity results.

Quando se estuda a qualidade física de meios porosos como o solo uma propriedade de bastante relevância é a sua densidade, ρs. As medidas de ρs são importantes devido à influência desta propriedade em outras deste sistema poroso (e.g., porosidade, umidade volumétrica, armazenamento de água, etc). Neste trabalho propõe-se uma metodologia alternativa para a medida de ρs, chamada de método da sela a vácuo. A impermeabilização da amostra, contrário aos métodos tradicionais como torrão parafinado, é feita utilizando embalagem plástica com posterior evacuação do ar interno usando bomba de vácuo. Medidas de ρs pelo método do torrão parafinado foram utilizadas como referência. Os resultados obtidos mostram que em relação ao método do torrão parafinado, ρs medido pelo método da sela a vácuo é, em média, subestimado para todos os diferentes tipos de solos analisados. O método proposto é mais rápido e apresenta maior confiabilidade na impermeabilização que o método tradicional, além disso, permite reutilizar a amostra para novas medidas. O novo método ainda pode ser facilmente implementado em laboratórios de ensino de física para o ensino médio e para o ensino superior nos cursos de bacharelado, engenharias e agronomia. Por se tratar de um método que envolve pouco manuseio, rápido e de fácil implementação, torna-se ideal para o estudo não somente de ρs, mas também de conceitos envolvidos em sua medida como massa, volume, massa específica, peso, peso aparente e empuxo.

When the physical quality of porous media such as soil is studied one of the properties of great importance is its bulk density, ρs. Measurements of ρs have great relevance due to its influence in other porous system properties (e.g. porosity, volumetric water content, water storage, etc.). In this paper is proposed an alternative methodology of ρs measurement named vacuum-sealed method. Samples coating, contrary to the traditional methods such as the paraffin-coated, was carried out using a plastic packaging followed by the sample internal air evacuation through a vacuum pump device. The determination of the clod samples ρs by the paraffin-coated method was utilized as a reference. The obtained results showed that compared to the paraffin-coated method, the average ρs measured by the vacuum-sealed method presented underestimated values considering all the soils analyzed. The proposed method is faster and has greater coating reliability than the traditional method with the additional advantage of sample reuse for new measurements. The new method can also be easily implemented in teaching physics laboratories in high school grade and bachelor’s, engineering, and environmental science courses. As a method that requires minimal sample preparation, easy to implement, and fast, it becomes ideal for the study not only of ρs but also to the discussion of concepts involved in its measurement such as mass, volume, weight, apparent weight and buoyancy force.