ABSTRACT Retaining the mineral N in the form of NH4+ in the soil for a lengthy period is desirable for reducing losses. Furthermore, there is evidence that sugarcane prefers NH4+-N in place of NO3−-N. This study aimed firstly, to evaluate the potential of root extracts of Bracchiaria humidicola andSaccharum spontaneum, in contrast with the DCD (Dicyandiamide) inhibitor, to increase absorption of N by plants fertilized with ammonium sulfate, and secondly, to quantify the emission of N2O fluxes with the use of this inhibitor. The experiment was developed in a glasshouse in an entirely randomized design where four treatments were applied: AS) ammonium sulfate (control); AS+DCD) ammonium sulfate associated with dicyandiamide; AS+BCH) ammonium sulfate associated with root extracts ofBrachiaria humidicola; and AS+SCS) ammonium sulfate associated with root extracts of Saccharum spontaneum. Differences were observed in biomass production in plants 45 and 60 days after fertilization (DAF) and 15 and 60 days in biomass accumulation of roots. The application of AS associated with DCD synthetic inhibitor kept NO3−-N values low throughout the evaluation period, while in other treatments the concentration increased right up to the second evaluation 15 DAF. Sugarcane plants did not benefit from the increased presence of ammoniacal N promoted by DCD. The use of DCD reduced the average flux of N2O during the evaluation period compared to plants receiving AS treatments only, which was not observed when root extracts of B. humidicola and S. spontaneum were used.

O estresse salino afeta o crescimento e a produtividade das culturas. Neste ensaio determinou-se o crescimento, o rendimento do fotossistema II (PSII) e a concentração de K+ e Na+ nas raízes, caules, folhas velhas e jovens das variedades mexicanas de arroz: Tres Ríos e Cotaxtla. Ademais, estudou-se a relação K+/Na+ na parte aérea e raízes de ambas variedades. O experimento foi conduzido em uma câmara de crescimento, sob condições controladas, em delineamento inteiramente ao acaso, com arranjo fatorial 2 x 2 (Variedade x Salinidade) e 12 repetições. As plantas foram crescidas em solução hidropônica durante 15 dias e posteriormente, uma parte foi tratada com 100 mM de NaCl; e o restante como plantas controle (sem tratamento com NaCl), as quais foram crescidas paralelamente. O estresse salino provocou reduções de 20 e 15% na longitude dos brotos e raízes, respectivamente na variedade Tres Ríos; entretanto, na variedade Cotaxtla não apresentaram diferenças significativas nestas variaveis com relação ao tratamento controle. O peso da matéria seca reduziu-se 24% na variedade Tres Ríos. O rendimento quântico do PSII diminuiu 30% ao terceiro dia da aplicação dos tratamentos em ambas variedades. A concentração de Na+ foi significativamente maior (p ≤ 0,05) nas plantas tratadas com NaCl. No entanto, na variedade Tres Ríos, o rendimento do PSII diminuiu completamente depois de seis dias da aplicação dos tratamentos, a concentração de K+ nos talos e folhas velhas também foi diminuída e registrou-se a menor relação K+/Na+ nos brotos, o que poderia indicar que se trata de uma variedade mais suscetível à salinidade com relação a variedade Cotaxtla.

Salt stress affects crop growth and productivity. In this study, we determined the growth, yield of photosystem II (PSII), and K+ and Na+ concentration in root, stem, old leaves, and young leaves of two Mexican varieties of rice, Tres Ríos and Cotaxtla. In addition, the K+/Na+ ratio in stem and root of both varieties was determined. The experiment was conducted in a growth chamber under controlled conditions, under a completely randomized distribution, with a 2 x 2 (Variety x Salinity) factorial arrangement and 12 replications. Plants were grown in a hydroponic solution for 15 days and then some of them were treated with 100 mM NaCl; control plants (without NaCI treatment) were grown in parallel. Salt stress caused 20 and 15% reductions in stem and root length, respectively, in the variety Tres Ríos, while in the variety Cotaxtla no significant differences were observed in these variables compared to the control. Dry matter weight decreased by 24% in the variety Tres Ríos. The quantum yield of PSII decreased by 30% the third day of treatment application, in both varieties. Na+ concentration was significantly (p ≤ 0.05) higher in NaCI-treated plants. In the variety Tres Ríos, the yield of PSII was completely eradicated six days after treatment implementation, while the K+ concentration in stem and older leaves also decreased and the lowest K+/ Na+ ratio in stem was recorded, which could indicate that it is more susceptible to salinity than the variety Cotaxtla.