ABSTRACT Hydrogen peroxide is a common reactive oxygen species involved in the catalytic mechanism though it is toxic to cells due to its oxidative nature. This work investigates the effects of hydrogen peroxide induced oxidative damage on bone mineral density and mechanical properties of bone which is primarily a composite material composed primarily of collagen fibers and biominerals. Sheep leg bones were exposed to hydrogen peroxide for a week. Bone mineral density was measured by using dual energy X-ray absorptiometry. Compressive modulus tests were applied to bone in order to determine mechanical properties. Our study shows that the hydrogen peroxide induced oxidative stress has negative effects on bone mineral density and stiffness. We observed higher control curve slopes than that of hydrogen peroxide curves which account for lesser stiffness values in the exposed tissue (p<0.05).