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au:CALVO, Adriana Maria

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Treatment of oral myiasis in a patient with implant-supported fixed prosthesis
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Sangalette, Beatriz Sobrinho

; Bortoloto, João Gabriel Perozo

; Pavoni, Rafaella Ferrari

; Cappellari, Valentina Interlichia

; Capelari, Marcos Mauricio

; Shinohara, André Luis

; Calvo, Adriana Maria

; Toledo, Gustavo Lopes

Revista do Instituto de Medicina Tropical de São Paulo

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2023, Volume 65 elocation e27

Abstract: En | Text: En | PDF: En

ABSTRACT Oral myiasis is a rare parasitic disease that requires immediate treatment once diagnosed. However, no standard treatment protocol can be found in the literature. Through a clinical-surgical report, we present the case of an 82-year-old man with lesions extending through the vestibule and alveolar ridge of the maxilla on both sides, in addition to occupying a large part of the palate, with a considerable number of larvae. The patient was initially treated with a single dose of systemic ivermectin (6 mg orally) and topical application of a tampon soaked in ether. The larvae were then surgically removed and debridement of the wound was performed. A crushed tablet of ivermectin 6 mg was applied topically for 2 days, the remaining larvae were again mechanically removed, and the patient received intravenous antimicrobial therapy. Treatment with systemic and topical ivermectin combined with antibiotic therapy and debridement proved to be effective in treating oral myiasis.

https://doi.org/10.1590/s1678-9946202365027

Pharmacogenetic and Pharmacokinetic Assays from Saliva Samples Can Guarantee Personalized Drug Prescription
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Bolani, Bruna

; Oliveira, Gabriela Moraes

; Dionísio, Thiago José

; Faria, Flavio Augusto Cardoso

; Fernandes, Maria Helena Raposo

; Santos, Carlos Ferreira

; Calvo, Adriana Maria

Brazilian Dental Journal

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feb 2021, Volume 32 N. 1 Pages 3 - 8

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Saliva é amplamente utilizada para análises clínicas e laboratoriais. Este estudo propôs o uso de DNA extraído da saliva para genotipagem e farmacocinética do piroxicam. Um método de genotipagem rápido e eficiente foi usado para determinar as variantes alélicas clinicamente relevantes de CYP2C9 (* 2 e * 3), uma vez que fatores genéticos podem influenciar nas respostas metabólicas individuais a medicamentos como anti-inflamatórios não esteroides (AINEs). DNA Extract All Reagents Kit® foi usado para extração de DNA e a genotipagem foi realizada usando TaqMan® GTXpress ™ Master Mix, ensaios de genotipagem SNP e um sistema Viia7 Real-Time PCR. Os voluntários realizaram coletas sequenciais de amostras de saliva antes e após a ingestão de uma única dose de piroxicam (0,25 a 72 h) que foram utilizadas para ensaios farmacocinéticos. As concentrações de piroxicam foram analisadas usando LC - MS / MS. Sessenta e seis por cento dos voluntários eram homozigotos ancestrais (CYP2C9 * 1 / * 1) e 34% apresentaram um ou ambos os polimorfismos. Destes 34%, 22 indivíduos apresentaram polimorfismo CYP2C9 * 2, 8 CYP2C9 * 3 e 4 CYP2C9 * 2 / * 3. A farmacocinética do piroxicam foi realizada em 5 indivíduos. As áreas sob a curva (AUC0-t (h * ng / mL)) para CYP2C9 * 1 / * 1, * 1 / * 2 e * 1 / * 3 foram, respectivamente, 194,33±70,93, 166 e 303. Concentrações máximas (Cmax (ng / mL)) para esses genótipos foram, respectivamente, 6,46±2,56, 4,3 e 10,2. A amostra de saliva foi uma matriz muito eficaz tanto para os testes farmacogenéticos quanto para os farmacocinéticos, garantindo a agilidade do procedimento e o bem-estar e concordância dos participantes. Com o conhecimento dos metabolizadores lentos e rápidos, é possível fazer uma prescrição adequada para evitar os efeitos adversos da medicação e garantir maior conforto analgésico aos pacientes respectivamente.

Abstract Saliva is widely used for clinical and laboratory analysis. This study proposed to use DNA extracted from saliva for genotyping and pharmacokinetics of piroxicam. A fast and efficient genotyping method was used to determine relevant allelic variants of CYP2C9 (*2 and *3), since genetic factors can influence in non-steroidal anti-inflammatory drugs (NSAIDs) metabolization. DNA Extract All Reagents Kit® was used for DNA extraction and genotyping was performed using TaqMan® GTXpress™ Master Mix, SNP genotyping assays and a Viia7 Real-Time PCR system. Volunteers performed sequential collections of saliva samples before and after taking a single dose of piroxicam (0.25 to 72 h) which were used for pharmacokinetics assays. Piroxicam concentrations were analyzed using LC-MS/MS. Sixty-six percent of volunteers were ancestral homozygous (CYP2C9*1/*1), and 34% showed one or both polymorphisms. Of these 34%, 22 individuals showed CYP2C9*2 polymorphism, 8 CYP2C9*3, and 4 CYP2C9*2/*3. Piroxicam pharmacokinetics were performed in 5 subjects. Areas under the curve (AUC0-t(h*ng/mL)) for CYP2C9*1/*1, *1/*2 and *1/*3 were, respectively, 194.33±70.93, 166 and 303. Maximum concentrations (Cmax(ng/mL)) for these genotypes were respectively 6.46±2.56, 4.3 and 10.2. Saliva sampling was a very effective matrix for both pharmacogenetic and pharmacokinetic tests, ensuring the speed of the procedure and the well-being and agreement of the participants. Once having the knowledge about the slow and fast metabolizers, it is possible to make an adequate prescription in order to avoid the adverse effects of the medication and to guarantee greater analgesic comfort to the patients respectively.

https://doi.org/10.1590/0103-6440202104059 804 downloads

Métodos e aspectos operacionais de um estudo epidemiológico e de avaliação da Rede Cegonha
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Boing, Antonio Fernando

; Lacerda, Josimari Telino de

; Boing, Alexandra Crispim

; Calvo, Maria Cristina Marino

; Saraiva, Suelen

; Tomasi, Yaná Tamara

; Natal, Sonia

; Pudla, Kátia Jakovljevic

; Danielewicz, Ana Lúcia

; Silva, Vilson Rodrigues da

; Nicolotti, Celia Adriana

; Machado, Patricia Maria de Oliveira

; Baratieri, Tatiane

Revista Brasileira de Epidemiologia

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2021, Volume 24 elocation e210010

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Objetivo: Descrever aspectos metodológicos e operacionais de um estudo epidemiológico e de avaliação da Rede Cegonha em Santa Catarina, Brasil. Métodos: A pesquisa realizada em 2019 foi composta de dois subestudos. No primeiro, cujo desenho foi epidemiológico e que analisou os cuidados recebidos no pré-natal, parto e puerpério imediato por gestantes, puérperas e crianças no Sistema Único de Saúde (SUS), são descritos os instrumentos de coleta dos dados e a organização da etapa de campo do estudo. O segundo foi uma avaliação normativa da gestão municipal na atenção ao pré-natal e puerpério no âmbito da Rede Cegonha. Iniciou-se com um estudo de avaliabilidade, seguido da avaliação propriamente dita. São descritas as diferentes estratégias metodológicas adotadas, com o envolvimento de stakeholders e especialistas. Resultados: A taxa de participação das mulheres entrevistadas no subestudo epidemiológico foi de 97,7%. Mulheres residentes em 82,7% dos municípios catarinenses foram entrevistadas. A amostra foi semelhante ao registrado no Sistema de Informações sobre Nascidos Vivos (SINASC) para o mesmo período, e o perfil da subamostra entrevistada após seis meses foi semelhante ao da amostra global. O estudo avaliativo desenvolveu e aplicou modelo com 32 indicadores, que permitiu analisar a gestão sob dois aspectos: político-organizacional e tático-operacional. Contou com a adesão de 204 municípios catarinenses (69,1%), avaliados segundo o porte populacional. Conclusão: A disponibilização de procedimentos metodológicos que possibilitem a articulação de estudos da epidemiologia e da avaliação em saúde permite gerar informações mais precisas e completas para contribuir para o delineamento e a avaliação de políticas, programas e ações de saúde do SUS.

ABSTRACT: Objective: To describe the methodological and operational aspects of an epidemiological and an evaluation of Rede Cegonha in Santa Catarina, Brazil. Methods: The research carried out in 2019 was composed of two sub-studies. Regarding the first, whose design was epidemiological and analyzed prenatal, delivery and immediate puerperium care addressed to pregnant women, puerperal women and children assisted at SUS, the instruments used for data collection and the organization of the field of the study are described. The other sub-study was a normative assessment of municipal management in prenatal and postpartum care within the scope of Rede Cegonha. It began with an evaluability assessment followed by the assessment itself. The different methodological strategies adopted are described, with the involvement of stakeholders and experts. Results: The response rate of the epidemiological sub-study was 97.7%. Women residing in 82.7% of Santa Catarina's municipalities were interviewed. The sample was similar to that registered in SINASC for the same period, and the characteristics of the sub-sample interviewed after six months was similar to the global sample. The evaluation study improved and applied a model with 32 indicators that allowed to analyze the municipalities considering the political-organizational and tactical-operational aspects. Two hundred and four municipalities answered the questionnaire (69.1%); they were evaluated according to their respective population size. Conclusion: The availability of methodological procedures of studies that articulate epidemiological and evaluation methods allows generating more accurate and complete information and contribute with the design and evaluation of health policies, programs and actions.

https://doi.org/10.1590/1980-549720210010

Métodos e aspectos operacionais de um estudo epidemiológico e de avaliação da Rede Cegonha
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Boing, Antonio Fernando

; Lacerda, Josimari Telino de

; Boing, Alexandra Crispim

; Calvo, Maria Cristina Marino

; Saraiva, Suelen

; Tomasi, Yaná Tamara

; Natal, Sonia

; Pudla, Kátia Jakovljevic

; Danielewicz, Ana Lúcia

; Silva, Vilson Rodrigues da

; Nicolotti, Celia Adriana

; Machado, Patricia Maria de Oliveira

; Baratieri, Tatiane

Revista Brasileira de Epidemiologia

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2021, Volume 24 elocation e210010

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/1980-549720210010 948 downloads

Molecular Response of Pulp Fibroblasts after Stimulation with Pulp Capping Materials
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Modena, Karin Cristina da Silva

; Calvo, Adriana Maria

; Sipert, Carla Renata

; Colombini-Ishikiriama, Bella Luna

; Dionísio, Thiago José

; Navarro, Maria Fidela de Lima

; Atta, Maria Teresa

; Santos, Carlos Ferreira

Brazilian Dental Journal

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jun 2020, Volume 31 N. 3 Pages 244 - 251

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Este estudo avalia in vitro a viabilidade e metabolismo celular, a liberação de óxido nítrico e a produção de duas quimiocinas e uma citocina por fibroblastos de polpa dentária humana em cultura (FPDH) em contato com dois cimentos de ionômero de vidro (Ketac Molar-KM e Vitrebond-VB), Single Bond (SB) e hidróxido de cálcio (Dycal-DY). As culturas de FPDH foram estabelecidas por meio de uma técnica de explante. As amostras foram preparadas em condições estéreis e em discos de 5 mm x 2 mm, obtidas de um molde pré-fabricado e colocadas em uma membrana permeável (Maxicell 24 W 0,4 µm) para evitar o contato direto com as células. A citotoxicidade foi avaliada pelo método de exclusão de Trypan Blue e pelo ensaio de MTT. A liberação de óxido nítrico no sobrenadante celular foi detectada pelo método Griess, enquanto fator 1 derivado do estroma (SDF-1α ou CXCL12), interleucina-8 (IL-8 ou CXCL8) and interleucina-6 (IL-6) foram detectados por ELISA. RT-qPCR foi empregada para análise de expressão gênica. As análises estatísticas foram realizadas por ANOVA a 1 critério, seguida pelo pós-teste de Tukey para os materiais independentes do tempo, e ANOVA a 2 critérios, seguida pelo teste de correção de Bonferroni para comparações entre materiais e tempo experimental (p<0,05). Os testes citotóxicos mostraram diferenças significativas apenas para DY. Os níveis da proteína e a expressão de RNAm para IL-8 aumentaram significativamente para ambos os tempos estudados. A produção de IL-6 aumentou quando os fibroblastos foram estimulados por KM. A produção da proteína e a expressão de RNAm para SDF-1α não foram afetadas por nenhum dos materiais. Houve uma diminuição nos níveis de nitrato/nitrito apenas para KM. Embora o DY tenha causado intensa morte celular e não tenha estimulado a produção dos mediadores inflamatórios avaliados neste trabalho, sabe-se que esse evento parece ser fundamental para o processo de reparo do tecido pulpar e formação de barreira mineralizada. Os cimentos de ionômero de vidro utilizados aumentaram a produção de proteínas relacionadas ao processo inflamatório, favorecendo a reparação tecidual e, portanto, esses materiais, embora não causem grande morte celular, devem ser utilizados com cautela.

Abstract This in vitro study evaluated cell viability and metabolism, nitric oxide release and production of two chemokines and one cytokine by cultured human dental pulp fibroblasts (HDPF) in contact with two glass ionomer cements (Ketac Molar-KM and Vitrebond-VB), Single Bond (SB) and calcium hydroxide (Dycal-DY). Cultures of HDPF were established by means of an explant technique. The specimens were prepared under sterile conditions and in disks measuring 5 mm x 2 mm obtained from a prefabricated mold and placed on a permeable membrane to avoid direct contact with the cells. Cytotoxicity was assessed by Trypan Blue exclusion method and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide release in cell supernatant was detected by the Griess Method whereas stromal derived factor-1 alpha (SDF-1α or CXCL12), chemokine (C-X-C motif) ligand 8 [Interleukin 8 (IL-8 or CXCL8)] and interleukin-6 (IL-6) were detected by ELISA. RT-qPCR was employed for gene expression analysis. Statistical analyses were performed by One-way ANOVA followed by Tukey’s post hoc test for materials independent of the time, and Two-way ANOVA followed by Bonferroni correction test for the comparisons between materials and experimental time (p<0.05). Cytotoxic tests showed significant differences only for DY. Protein levels and mRNA expression were significantly increased for IL-8 for both periods of time. IL-6 production increased when fibroblasts were stimulated by KM. SDF-1α protein production and mRNA expression were not affected by any of the materials. There was a decrease in nitrate/nitrite levels only for KM. Although DY caused intense cell death and did not stimulate the production of the inflammatory mediators evaluated in this work, it is known that this event seems to be fundamental for the process of repair of the pulp tissue and formation of mineralized barrier. KM and VB increased production of proteins related to the inflammatory process, thus favoring tissue repair. Therefore, although these glass ionomer cements did not lead to large cell death, they should be used with caution.

https://doi.org/10.1590/0103-6440202003523 1057 downloads

Randomized trial of physiotherapy and hypertonic saline techniques for sputum induction in asthmatic children and adolescents
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Felicio-Júnior, Egberto Luiz

; Barnabé, Viviani

; de Almeida, Francine Maria

; Avona, Monise Dematte

; de Genaro, Isabella Santos

; Kurdejak, Adriana

; Eller, Miriam Cardoso Neves

; Vergani, Karina Pierantozzi

; Rodrigues, Joaquim Carlos

; Tibério, Iolanda de Fátima Lopes Calvo

; Martins, Milton de Arruda

; Saraiva-Romanholo, Beatriz Mangueira

Clinics

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2020, Volume 75 elocation e1512

Abstract: En | Text: En | PDF: En

OBJECTIVES: This study aimed to analyze the efficiency of physiotherapy techniques in sputum induction and in the evaluation of pulmonary inflammation in asthmatic children and adolescents. Although hypertonic saline (HS) is widely used for sputum induction (SI), specific techniques and maneuvers of physiotherapy (P) may facilitate the collection of mucus in some asthmatic children and adolescents. METHODS: A randomized crossover study was performed in patients with well-controlled asthma, and 90 sputum samples were collected. Children and adolescents were assessed using spirometry and randomized at entry into one of three sputum induction techniques: (i) 3% hypertonic saline – HS technique; (ii) physiotherapy (oscillatory positive expiratory pressure, forced expiration, and acceleration of expiratory flow) – P technique; and (iii) hypertonic saline + physiotherapy – HSP technique. ClinicalTrials.gov: NCT03136042. RESULTS: The total cells (mL) and the percentage (%) of differential inflammatory cells were similar in all techniques. The sputum weight (g) in the HSP technique was significantly higher than that in the HS technique. In all techniques, the percentage of viable cells was >50%, and there was no difference between the HS and P techniques. Moreover, sputum induction did not cause any alterations in the pulmonary function of patients. CONCLUSION: The physiotherapy sputum collection technique was effective in obtaining viable cells from mucus samples and yielded the same amount of sputum as the gold standard technique (hypertonic saline). In addition, the physiotherapy maneuvers were both safe and useful for sputum induction in asthmatic children and adolescents with well-controlled asthma.

https://doi.org/10.6061/clinics/2020/e1512 1169 downloads

ACTIVIDAD ANTIMICROBIANA DE WIRA WIRA Y CERRAJA CONTRA ESTAFILOCOCO, ENTEROCOCO, PSEUDOMONAS Y ESCHERICHIA
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Anaya Muñoz, Santiago Franklin

; Calvo Orellana, Eldy Ericka

; Valdez Ramallo, María Alexandra

; Santa Cruz Rodriguez, Adriana C.

Revista Científica Ciencia Médica

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2020, Volume 23 N. 1 Pages 15 - 21

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Introducción: El uso de plantas con diversos fines etnomedicinales es una práctica ancestral y actualmente común en una vasta parte de la población de Bolivia. Objetivo general: Identificar si la wira wira y cerraja de nuestro medio poseen actividad antimicrobiana contra bacterias patógenas y determinar su toxicidad a través del bioensayo de pruebas biológicas. Materiales y Métodos: Estudio descriptivo; Universo, Wira Wira recolectada del Valle Alto de Cochabamba y Cerraja de Cercado; Muestra, se obtuvo de forma aleatoria un kilogramo de Wira Wira y Cerraja respectivamente; Métodos, se emplearon diferentes técnicas para la extracción de los principios activos, se realizaron pruebas biológicas para determinar la toxicidad de los mismos y se determinó la actividad antimicrobiana por el método de difusión en agar. Resultados: Wira wira demostró actividad antimicrobiana con 4 extractos; alcohólico de tallo, flor y alcohólico agotado de hojas frente a E. faecalis y S. aureus, y acuoso de hojas frente a P. aeruginosa y S. aureus. Por otro lado no se evidencio actividad antibacteriana de los extractos de cerraja. Conclusiones: Wira wira es candidata para estudios posteriores destinados a la identificación del o los compuestos activos puros con actividad antibacteriana, hecho respaldado por los resultados obtenidos en esta y otras investigaciones. Los extractos de cerraja, a diferencia de los resultados obtenidos de otros trabajos, no presentaron actividad antimicrobiana, demostrando la posibilidad y vinculación de la variación en los compuestos de la planta con el ecosistema en el que se desarrolla.

Introduction: The use of plants with various ethnomedicinal purposes is an ancestral practice and currently common in a vast part of the population of Bolivia. General objective: To identify if the extracts of both plants present in our environment have antimicrobial activity against pathogenic bacteria and to determine their toxicity through the bioassay of biological tests. Materials and Methods: Descriptive study; Universe, Wira Wira collected from Valle Alto in Cochabamba and Cerraja collected from Cercado; Sample, we obtained one kilogram of each, Wira Wira and Cerraja randomly; Methods, different techniques were used to extract the active ingredients, biological tests were performed to determine their toxicity and antimicrobial activity was determined by the agar diffusion method. Results: Wira Wira demonstrated antimicrobial activity with 4 extracts; stem alcoholic, flower and alcoholic depleted of leaves against E. faecalis and S. aureus, and aqueous of leaves against P. aeruginosa and S. aureus. On the other hand, there was no evidence of antibacterial activity with the cerraja extracts. Conclusions: Wira Wira is a candidate for further studies aimed at the identification of the pure active compound (s) with antibacterial activity, all this backed up by the positive results in the agar diffusion tests grown with pathogenic bacteria. Unlike the results that demonstrate the antibacterial activity of the Cerraja in research carried out in other parts of the world, it is not the case of the one in our territory, whose extracts did not present any antimicrobial activity, demonstrating the possibility and linking the variation in plant compounds with the ecosystems in which it develops.

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Dental Pulp Fibroblasts Response after Stimulation with HEMA and Adhesive System
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Modena, Karin Cristina da Silva

; Calvo, Adriana Maria

; Sipert, Carla Renata

; Dionísio, Thiago José

; Navarro, Maria Fidela de Lima

; Atta, Maria Teresa

; Santos, Carlos Ferreira

Brazilian Dental Journal

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sep 2018, Volume 29 N. 5 Pages 419 - 426

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Este estudo avaliou in vitro a viabilidade e metabolismo celular, liberação de óxido nítrico e produção de quimiocinas em cultura de fibroblastos de polpa dental humana (DPF) em contato com HEMA e Single Bond. Culturas de DPF foram estabelecidas por meio de uma técnica de explante. Uma vez plaqueadas, as células foram mantidas em contato com concentrações crescentes de HEMA (10, 100 e 1000 nM) ou Single Bond (SB) [10 vezes diluídas em série em meio de cultura (10-4, 10-3 e 10-2 v/v)] e também com SB polimerizado. A citotoxicidade foi avaliada pelo método de exclusão de Trypan Blue e pelo ensaio de 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazólio brometo (MTT). A liberação de óxido nítrico no sobrenadante celular foi detectada pelo método de Griess, enquanto as quimiocinas (CXCL12 e CXCL8) foram detectadas por ELISA. RT-qPCR foi empregada para análise de expressão gênica de quimiocinas. Testes citotóxicos mostraram diferenças significativas para SB 10-2. Nenhum dos materiais testados alterou significativamente os níveis de NO. Os níveis de proteína de CXCL12 foram significativamente diminuídos apenas pelo HEMA. Por outro lado, enquanto o RNAm de CXCL12 permaneceu inalterado, a expressão gênica de CXCL8 teve redução significativa com todos os materiais, com exceção do SB polimerizado. Em conclusão, Single Bond e HEMA, em várias concentrações, diminuíram a expressão e produção de moléculas envolvidas em processos inflamatórios e, portanto, o uso de sistemas adesivos, como o material protetor da polpa, deve ser visto com cautela devido ao seu grande efeito citotóxico quando em contato com a polpa.

Abstract This study evaluated in vitro cell viability and metabolism, nitric oxide release and production of chemokines by cultured human dental pulp fibroblasts (DPF) under contact with HEMA and Single Bond. Cultures of DPF were established by means of an explant technique. Once plated, cells were kept under contact with increasing concentrations of HEMA (10, 100 and 1000 nM) or Single Bond (SB) [10-fold serially diluted in culture medium (10-4, 10-3 and 10-2 v/v)] and also with polymerized SB components. Cytotoxicity was assessed by Trypan Blue exclusion method and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Nitric oxide release on cell supernatant was detected by Griess Method whereas chemokines (CXCL12 and CXCL8) were detected by ELISA. RT-qPCR was employed for chemokines gene expression analysis. Cytotoxic tests showed significant differences for SB 10-2. None of the tested materials significantly altered NO levels. Protein levels of CXCL12 were significantly decreased only by HEMA. On the other hand, while CXCL12 mRNA remained unaltered, gene expression of CXCL8 had significant decrease with all materials, except for polymerized SB. In conclusion, Single Bond and HEMA at various concentrations, decreased expression and production of molecules involved in inflammatory processes and, therefore, the use of adhesive systems such as pulp capping materials must be viewed with caution due to its large cytotoxic effect when in close contact with the pulp.

https://doi.org/10.1590/0103-6440201802558 1308 downloads

Efficacy of Ketoprofen With or Without Omeprazole for Pain And Inflammation Control After Third Molar Removal
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Simoneti, Luis Fernando

; Weckwerth, Giovana Maria

; Dionísio, Thiago José

; Torres, Elza Araujo

; Zupelari-Gonçalves, Paulo

; Calvo, Adriana Maria

; Lauris, José Roberto

; Faria, Flávio Cardoso

; Santos, Carlos Ferreira

Brazilian Dental Journal

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mar 2018, Volume 29 N. 2 Pages 140 - 149

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Em vista dos problemas gastrointestinais gerados pelo uso do cetoprofeno, a associação do cetoprofeno com o omeprazol está disponível no mercado. No entanto, esta eficácia de associação no controle da dor aguda não foi estabelecida. A extração bilateral de terceiros molares inferiores em posições semelhantes é atualmente o modelo mais utilizado para a avaliação e investigação da eficácia e efeitos farmacológicos de novos compostos para o tratamento da dor aguda pós-operatória. O estudo randomizado e cruzado consistiu na avaliação da eficácia clínica da terapia com cetoprofeno 100 mg (duas vezes ao dia-b.i.d.) versus cetoprofeno 200 mg + omeprazol 20 mg (uma vez ao dia-q.d.) para o controle da dor, do edema e do trismo no modelo bilateral de terceiros molares inferiores em posições semelhantes em duas consultas diferentes, em 50 voluntários. Os voluntários relataram significativamente menos dor pós-operatória em vários períodos pós-operatórios e consumiram menos medicação analgésica de socorro (acetaminofeno 750 mg) durante todo o estudo quando tomaram a combinação de 200 mg de cetoprofeno + 20 mg de omeprazol (q.d.). Após a administração de ambas as drogas do estudo, nenhuma reação adversa gastrointestinal foi relatada pelos voluntários. Além disso, as avaliações das drogas no controle da dor pelos voluntários foram significativamente favoráveis ao cetoprofeno 200 mg + omeprazol 20 mg (q.d.). Para o controle do edema e do trismo, os tratamentos apresentaram resultados semelhantes. Em conclusão, quando os voluntários tomaram 200 mg de cetoprofeno + 20 mg de omeprazol (q.d.), eles relataram significativamente menos dor pós-operatória em vários períodos pós-cirúrgicos e consumiram menos medicação analgésica de socorro durante o estudo comparado com 100 mg de cetoprofeno (b.i.d).

Abstract In view of the gastrointestinal problems generated by the ketoprofen use, the ketoprofen association with omeprazole is available on the market. However, this association efficacy in acute pain control has not been established. Bilateral extraction of lower third molars in similar positions is currently the most used model for the evaluation and investigation of the efficacy and pharmacological effects of new compounds for the treatment of acute postoperative pain. The randomized and crossover study consisted in evaluating the clinical efficacy of therapy performed by ketoprofen 100 mg (twice daily-b.i.d.) versus ketoprofen 200 mg + omeprazole 20 mg (once daily-q.d.) to pain, swelling and trismus control in the bilateral extraction model of lower third molars in similar positions in two different appointments, in 50 volunteers. Volunteers reported significantly less postoperative pain at various post-operative periods and consumed less rescue analgesic medication (acetaminophen 750 mg) throughout the study when they took the combination of ketoprofen 200 mg + omeprazole 20 mg (q.d.). Following administration of both study drugs, no gastrointestinal adverse reactions were reported by volunteers. Furthermore, the evaluations of the drugs in pain control by the volunteers were significantly favorable to ketoprofen 200 mg + omeprazole 20 mg (q.d.). For swelling and trismus control, the treatments presented similar results. In conclusion, when volunteers took ketoprofen 200 mg + omeprazole 20 mg (q.d.), they reported significantly less postoperative pain at various post-surgical periods and consumed less rescue analgesic medication throughout the study compared with ketoprofen 100 mg (b.i.d).

https://doi.org/10.1590/0103-6440201802254 2627 downloads

10.

Effective method for the detection of piroxicam in human plasma using HPLC
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CALVO, Adriana Maria

; PRADO, Mariel Tavares de Oliveira

; DIONÍSIO, Thiago José

; MARQUES, Maria Paula

; BROZOSKI, Daniel Thomas

; LANCHOTE, Vera Lúcia

; FARIA, Flávio Augusto Cardoso

; SANTOS, Carlos Ferreira

Brazilian Oral Research

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2016, Volume 30 N. 1 elocation e58

Abstract: En | Text: En | PDF: En

Abstract Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used by the general population to alleviate inflammation and pain after oral surgeries. Piroxicam is among the most commonly used NSAIDs and excels in controlling pain, swelling, trismus and other common symptoms of inflammation. This study aimed to evaluate different concentrations of piroxicam and its major metabolite, 5’-hydroxypiroxicam, in human plasma samples over time using high performance liquid chromatography (HPLC) after liquid-liquid extraction. Briefly, 10 volunteers participated in this study after approval by the Ethics Committee of Bauru School of Dentistry, Universidade de São Paulo – USP, Brazil. Volunteers received a single dose oral of piroxicam (20 mg) and had blood collected at various times following an established protocol. The methodology of liquid-liquid extraction was effective for determining concentrations of piroxicam in plasma using HPLC in 10 out of 10 volunteers while 5’-hydroxypiroxicam was only detected in 2 out of 10 volunteers.

https://doi.org/10.1590/1807-3107BOR-2016.vol30.0058 2562 downloads

11.

Realidad virtual como buena práctica para trabajo en equipo con estudiantes de ingeniería
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Gasca-Hurtado, Gloria Piedad

; Peña, Adriana

; Gómez-Álvarez, María Clara

; Plascencia-Osuna, Óscar Armando

; Calvo-Manzano, Jose A.

RISTI - Revista Ibérica de Sistemas e Tecnologias de Informação

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dec 2015, N. 16 Pages 76 - 91

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

La realidad virtual es una alternativa de motivación de los estudiantes al darles la posibilidad de realizar acciones como volar u observar el entorno desde diferentes perspectivas de manera controlada. La interacción multiusuario en realidad virtual da lugar a los entornos virtuales colaborativos que favorecen el desarrollo de habilidades sociales como el trabajo en equipo, en particular en equipos con integrantes con ubicaciones geográficas diferentes. En estos contextos se encuentran diversas propuestas de aplicación de la realidad virtual que transportan a los estudiantes a entornos donde deben tomar decisiones conjuntas para lograr los objetivos propuestos. Sin embargo, estas propuestas no hacen énfasis en la importancia de reconocer las fortalezas o habilidades de cada miembro del equipo para mejorar su desempeño. El presente artículo propone un videojuego, basado en realidad virtual, que incorpora los principios de gamificación para promover y mejorar el trabajo en equipo en estudiantes de ingeniería.

Virtual reality is an alternative for student's motivation, allowing them actions like flying or observing the environment from different perspectives in a controlled way. Multiuser interaction in virtual reality leads to collaborative virtual environments that promote the development of social skills in teamwork, in particular with members in different geographic locations. In these contexts there are different virtual reality application proposals transporting students to environments where they make team decisions for achieving intended goals. However, such proposals do not emphasize the importance of identifying the strengths of each team member for the improvement in the team performance. This paper presents a virtual reality-based videogame incorporating gamification principles for promoting and improving teamwork in engineering students.

https://doi.org/10.17013/risti.16.76-91 4852 downloads

12.

Avaliação do estado nutricional de idosos institucionalizados
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Rauen, Michelle Soares

; Moreira, Emília Addison Machado

; Calvo, Maria Cristina Marino

; Lobo, Adriana Soares

Revista de Nutrição

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jun 2008, Volume 21 N. 3 Pages 303 - 310

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Identificar o estado nutricional, segundo o índice de massa corporal, e fornecer informações sobre medidas antropométricas de idosos institucionalizados no município de Florianópolis (SC). MÉTODOS: Estudo transversal, no qual a população em estudo foi composta por 232 idosos, com idade a partir de 60 anos, residentes em instituições geriátricas do município de Florianópolis (SC). A avaliação antropométrica foi realizada obtendo-se o valor do índice de massa corporal, da prega cutânea triciptal e da circunferência da panturrilha. O diagnóstico do estado nutricional foi realizado utilizando-se os pontos de corte preconizados pela Organização Pan-Americana de Saúde para índice de massa corporal. Os dados são apresentados sob a forma de média e desvio-padrão. Foi realizado o teste t de Student para verificar a diferença entre os valores médios das variáveis entre os sexos. RESULTADOS: A amostra constituiu-se de 167 idosos de ambos os sexos. Encontrou-se uma prevalência de 45,5% de baixo peso, 33,5% de peso normal, 7,8% de pré-obesidade e 13,2% de obesidade. Para todas as variáveis antropométricas, o valor médio das mulheres foi superior ao dos homens. CONCLUSÃO: Os resultados encontrados indicaram a prevalência de 66,5% da amostra com estado nutricional inadequado, evidenciando a necessidade de medidas de promoção ou reabilitação da saúde dos idosos. Os dados mostram que há diferenças entre os sexos com relação ao estado nutricional e às variáveis índice de massa corporal e prega cutânea triciptal, além de contribuírem para a ampliação do referencial antropométrico de idosos institucionalizados.

OBJECTIVE: The objective of this study was to identify the nutritional status, according to body mass index, and provide information regarding anthropometric measurements of institutionalized seniors in the city of Florianópolis (SC), Brazil. METHODS: This is a cross-sectional study. The research population consisted of 232 senior citizens, aged 60 or more, living in geriatric institutions in Florianópolis (SC), Brazil. Anthropometric assessment included determining the body mass index, tricipital skinfold, and calf circumference. Diagnosis of the nutritional status was performed using the cut-off points determined by the Pan-American Health Organization for body mass index. The data are presented as means and standard deviation. The Student's " t" test was used to detect differences in mean values of the variables between both genders. RESULTS: The sample consisted of 167 senior citizens of both genders. A prevalence of 45.5% of low weight, 33.5% of normal weight, 7.8% of overweight and 13.2% of obesity was observed. The mean values for women were higher than those for men for all anthropometric variables. CONCLUSION: The data obtained showed that 66.5% of the sample presented inadequate nutritional status, clearly evidencing the need to take some measures that promote or recover the health of senior citizens. In addition, the data obtained show that there are differences between both genders regarding the nutritional status and the variables body mass index and tricipital skinfold and contribute to increase the anthropometric references of institutionalized senior citizens.

29625 downloads Cited 9 times in SciELO

13.

Reprodutibilidade de um questionário de consumo alimentar para crianças em idade escolar
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Lobo, Adriana Soares

; Assis, Maria Alice Altenburg de

; Barros, Mauro Virgílio Gomes de

; Calvo, Maria Cristina Marino

; Freitas, Sérgio Fernando Torres

Revista Brasileira de Saúde Materno Infantil

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mar 2008, Volume 8 N. 1 Pages 55 - 63

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVOS: avaliar a reprodutibilidade de um questionário para obter auto-relato de 16 alimentos consumidos em cinco refeições num dia típico da semana, em escolares do ensino fundamental. MÉTODOS: participaram do estudo 342 escolares das primeiras às quartas séries do ensino fundamental (duas escolas públicas e três privadas) de Florianópolis, Santa Catarina, Brasil. A reprodutibilidade foi determinada através da comparação das respostas obtidas em dois dias, após um intervalo de três dias. Análises foram estratificadas por série escolar (primeiras e segundas versus terceiras e quartas) e rede escolar (pública e privada), para determinar, o percentual de concordância (%CC) e estatística kappa ajustada. RESULTADOS: nas escolas públicas, os valores de %CC variaram entre 69% (pães) e 84,5% (peixes) e os valores de kappa entre 0,38 (pães) e 0,69 (peixes). Nas escolas privadas os %CC variaram entre 73,9% (pães) e 91,6% (feijão) e os valores de kappa entre 0,48 (pães) e 0,83 (feijão). Maiores valores de %CC e de kappa foram obtidos para as terceiras e quartas séries do que para as primeiras e segundas, nas escolas públicas e privadas. CONCLUSÕES: a reprodutibilidade do questionário foi demonstrada para os escolares de escolas privadas e públicas, embora melhores resultados foram obtidos nas escolas privadas.

OBJECTIVES: to assess the reproducibility of a questionnaire to obtain self-recall of 16 foods eaten in five meals on a typical day of the week in elementary-school children. METHODS: the study subjects were 342 schoolchildren from the first to fourth grade of elementary schools (two public and three private) in the city of Florianópolis, State of Santa Catarina, Brazil. Reproducibility was determined by comparing responses obtained on two separate days, within a 3-day interval. Analyses were stratified according to school grade (first and second versus third and fourth) and type of school (public and private) to assess: the percentage agreement (%CC) and the adjusted kappa statistic. RESULTS: in public schools, %CC values ranged from 69% (bread) to 84.5% (fish), and kappa values from 0.38 (bread) to 0.69 (fish). In private schools, %CC values ranged from 73.9% (bread) to 91.6% (beans) and kappa values from 0.48 (bread) to 0.83 (beans). In analyses stratified according to grade, %CC and kappa values were better for third and fourth grades compared to first and second grades in both public and private schools. CONCLUSIONS: the reproducibility of the questionnaire was demonstrated for elementary schoolchildren in private and public schools, but the former presented better results.

6525 downloads Cited 3 times in SciELO

14.

Modelo de Markov de tres estados: comparación de parametrizaciones de la tasa de intensidad de transición. Aplicación a datos de artritis reumatoidea
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SALAZAR, JUAN CARLOS

; PALOMINO, RENÉ IRAL

; CALVO, ENRIQUE

; ROJAS, ADRIANA

; HINCAPIÉ, MARÍA EUGENIA

; ANAYA, JUAN MANUEL

; DÍAZ, FRANCISCO JAVIER

Revista Colombiana de Estadística

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dec 2007, Volume 30 N. 2 Pages 213 - 229

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Se considera un modelo múltiple de tres estados donde uno de ellos es absorbente. Se asume que la dependencia entre las observaciones registradas para un mismo sujeto sigue un proceso de Markov. Se comparan, vía simulación, tres diferentes parametrizaciones de la tasa de intensidad de transición: la primera está basada en el modelo de hazard multiplicativo de Andersen-Gill (Andersen et al. 1993), la segunda, en el modelo logístico, y la tercera depende del modelo log-log complementario. El método de estimación de parámetros se basa en la función de verosimilitud la cual se optimiza usando las soluciones exactas de un sistema de ecuaciones de Kolmogorov hacia adelante junto con el algoritmo de Newton-Raphson (Abramowitz & Stegun 1972). Usando el sesgo relativo, se selecciona el mejor método de parametrización y se ilustra usando datos recopilados en la Corporación para Investigaciones Biológicas, CIB, acerca de pacientes con artritis reumatoidea.

We consider a three state model with an absorbing state assuming an underlying Markov process to explain the dependence among observations within subjects. We compare, using a simulation study, three different parameterizations of the transition intensity rate: the first one is based on the Andersen-Gills multiplicative hazard model (Andersen et al. 1993), the second one is based on the logistic model, and the third one depends on the complementary log-log model. The method to estimate the effect of the parameters is based on the likelihood function which can be optimized using the exact solutions of a Kolmogorov forward differential equations system in conjunction with the Newton-Raphson algorithm (Abramowitz & Stegun 1972). We use the relative bias to select the best estimation estrategy. The methodology is ilustrated using longitudinal data about rheumatoid arthritis (RA) from the Corporación para Investigaciones Biológicas, CIB.

1416 downloads

15.

PERSPECTIVES OF MARINE BIODIVERSITY STUDIES IN ARGENTINA
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Lutz, Vivian A

; Boschi, Enrique E

; Bremec, Claudia S.

; Cousseau, María Berta

; Figueroa, Daniel E.

; Rodríguez, Diego H.

; Scarlato, Norberto

; Viñas, María Delia

; Lewis, Mirtha N.

; Penchaszadeh, Pablo E.

; Acuña, Fabián H

; Aguirre, Marina L.

; Akselman, Rut

; Alder, Viviana A.

; Baldoni, Ana

; Barría, María S.

; Bastida, Ricardo O.

; Boraso, Alicia S.

; Calvo, Jorge

; Campagna, Claudio

; Cañete, Guillermo

; Cassia, María C.

; Cervellini, Patricia M.

; Chiaramonte, Gustavo E.

; Costagliola, Marcela

; Cosulich, Guillermina

; Daponte, María C.

; Díaz de Astarloa, Juan M.

; Elías, Rodolfo

; Esnal, Graciela B.

; Excoffon, Adriana C.

; Freije, Hugo

; García de la Rosa, Susana

; Genzano, Gabriel N.

; Giberto, Diego A.

; Guerrero, Raúl

; Helbling, Walter

; Hoffmeyer, Mónica S.

; Incorvaia, Inés S.

; Iribarne, Oscar

; Jaureguizar, Andrés J.

; Kogan, Mariela

; Lovrich, Gustavo A

; Madirolas, Adrián O.

; Martin, Juan P.

; Martos, Patricia

; Mendoza, María L.

; Marque, Silvina Menu

; Mianzán, Hermes W.

; Morriconi, Elba R.

; Negri, Rubén M.

; Obenat, Sandra

; Pastor de Ward, Catalina T.

; Pérez de Fankhauser, Laura B.

; Quintana, Flavio

; Reta, Raúl

; Rico, Rita

; Roux, Ana M.

; Sabatini, Marina E.

; Scelzo, Marcelo A.

; Schejter, Laura

; Schiavini, Adrián C.M.

; Silva, Ricardo I.

; Silvoni, María G.

; Spivak, Eduardo

; Trucco, María I.

; Vallarino, Eduardo A.

; Zelaya, Diego G.

Gayana (Concepción)

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2003, Volume 67 N. 2 Pages 371 - 382

Abstract: En | Text: En | PDF: En

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